Zebo Li, Qihua Shan, Jian-feng Zhang, Guangcai Chen, Y. Wang, Xianhu Liu
The copper mine tailing dam in Tongling city has highly potential environment risk. To recognize the underground distribution of Cu in the mine tailing dam, nine profiles were excavated and surveyed in the central area of the deserted copper tailing dam. Soil samples from different layers of each profile were collected separately. The copper content in the tailing dam exceeded the toxic threshold of plants much, indicating high potential risk to local environment. The Copper of total and available contents both had the high heterogeneity in the horizontal direction. In the vertical direction, the total and available form of Cu displayed an opposite trend. The copper in the surface soil was easier to resolve, transport and transform than that in the subsoil.
{"title":"Soil Copper Distribution in Tongling Mine Tailing Dam, China","authors":"Zebo Li, Qihua Shan, Jian-feng Zhang, Guangcai Chen, Y. Wang, Xianhu Liu","doi":"10.1109/ICBEB.2012.357","DOIUrl":"https://doi.org/10.1109/ICBEB.2012.357","url":null,"abstract":"The copper mine tailing dam in Tongling city has highly potential environment risk. To recognize the underground distribution of Cu in the mine tailing dam, nine profiles were excavated and surveyed in the central area of the deserted copper tailing dam. Soil samples from different layers of each profile were collected separately. The copper content in the tailing dam exceeded the toxic threshold of plants much, indicating high potential risk to local environment. The Copper of total and available contents both had the high heterogeneity in the horizontal direction. In the vertical direction, the total and available form of Cu displayed an opposite trend. The copper in the surface soil was easier to resolve, transport and transform than that in the subsoil.","PeriodicalId":6374,"journal":{"name":"2012 International Conference on Biomedical Engineering and Biotechnology","volume":"29 1","pages":"1672-1675"},"PeriodicalIF":0.0,"publicationDate":"2012-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85098671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Zhong-qiu Li, Jian-zhang Ma, Chunlong Liu, Di Liu, Fugang Peng, Hong Ma, B. Fu, Liang Wang, Jinyan Sun, Wen-tao Wang
The northeastern wild boar ear skin fibroblasts line was successfully established using the primary explant technique. Study on the ear skin fibroblasts of the northeastern wild boar isolation, Subculture, cryopreservation and growth characteristics. Observations on morphology and dynamic growth.
{"title":"Isolation and Culture of Northeastern Wild Boar Ear Skin Fibroblasts","authors":"Zhong-qiu Li, Jian-zhang Ma, Chunlong Liu, Di Liu, Fugang Peng, Hong Ma, B. Fu, Liang Wang, Jinyan Sun, Wen-tao Wang","doi":"10.1109/ICBEB.2012.258","DOIUrl":"https://doi.org/10.1109/ICBEB.2012.258","url":null,"abstract":"The northeastern wild boar ear skin fibroblasts line was successfully established using the primary explant technique. Study on the ear skin fibroblasts of the northeastern wild boar isolation, Subculture, cryopreservation and growth characteristics. Observations on morphology and dynamic growth.","PeriodicalId":6374,"journal":{"name":"2012 International Conference on Biomedical Engineering and Biotechnology","volume":"20 1","pages":"1045-1047"},"PeriodicalIF":0.0,"publicationDate":"2012-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81604058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Every liver mitochondria protein sample from the normal rats and rats infected with hydrocortisone was labeled with Cy3 or Cy5 randomly. Each Cy3-labeled sample and Cy5-labeled sample were mixed on the same 2-D gel along with a Cy2-labeled mixture of all samples as an internal standard. The gels were respectively imaged by Typhoon 9400 using different emission filter and spot-features were analyzed by DeCyder5.0 software in each paired normal/disease comparison. 11 different expression proteins were selected, in which 7 proteins were successfully identified. In their proteins, the expression of Glucose regulated protein (grp75), heat shock protein 60, carbamoyl-phosphate synthetase 1, ATP synthetase, sarcosine dehydrogenase, chaperone protein, dimethylglycine dehydrogenase increased while the expression of adenylate kinas4 decreased. These result showed that the metabolism of substance was affected by hydrocortisone. The expression of heat shock protein and Glucose regulated protein increased, which contributed to protect the cell from damage. The metabolism of protein increased and the energy metabolism was alleviated to increase the output of ATP. However, the stability of energy metabolism and the ability of self-regulation were disturbed when the rats were injected with hydrocortisone.
{"title":"Effect of Hydrocortisone on Liver Mitochondria Proteome of Rats","authors":"D. Lu, Ping-Er Wang, Ji Zhu, Tao-Qi Lin, X. Wo","doi":"10.1109/ICBEB.2012.168","DOIUrl":"https://doi.org/10.1109/ICBEB.2012.168","url":null,"abstract":"Every liver mitochondria protein sample from the normal rats and rats infected with hydrocortisone was labeled with Cy3 or Cy5 randomly. Each Cy3-labeled sample and Cy5-labeled sample were mixed on the same 2-D gel along with a Cy2-labeled mixture of all samples as an internal standard. The gels were respectively imaged by Typhoon 9400 using different emission filter and spot-features were analyzed by DeCyder5.0 software in each paired normal/disease comparison. 11 different expression proteins were selected, in which 7 proteins were successfully identified. In their proteins, the expression of Glucose regulated protein (grp75), heat shock protein 60, carbamoyl-phosphate synthetase 1, ATP synthetase, sarcosine dehydrogenase, chaperone protein, dimethylglycine dehydrogenase increased while the expression of adenylate kinas4 decreased. These result showed that the metabolism of substance was affected by hydrocortisone. The expression of heat shock protein and Glucose regulated protein increased, which contributed to protect the cell from damage. The metabolism of protein increased and the energy metabolism was alleviated to increase the output of ATP. However, the stability of energy metabolism and the ability of self-regulation were disturbed when the rats were injected with hydrocortisone.","PeriodicalId":6374,"journal":{"name":"2012 International Conference on Biomedical Engineering and Biotechnology","volume":"8 1","pages":"202-205"},"PeriodicalIF":0.0,"publicationDate":"2012-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81612061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The effect of fibrillation earthworm feeding on degradation of dissolved substances has been studied by the intermittent test. As the earthworm density changes, the conversion of certain substances in the activated sludge system, including the sludge activity, ammonia nitrogen and COD treatment effect has been investigated. From the perspective of predation, it may reduce the removal efficiency of system for NH4+-N when insect fibrillation earthworm body present in the reactor because of the feeding effect on nitrifying bacteria. The results show that there is some impact on the degradation rate of ammonia degradation for the presence of fibrillation earthworm, but little for the removal effect of final ammonia and COD, especially the final ammonia. And by measuring the value of OUR, the right flutter earthworm density will play an active role in bacteria in the activated sludge system.
{"title":"The Effect of Fibrillation Earthworm on Sludge Treatment in the Activated Sludge System","authors":"Chun-di Gao, Li Wang, Tianqi Yang","doi":"10.1109/ICBEB.2012.416","DOIUrl":"https://doi.org/10.1109/ICBEB.2012.416","url":null,"abstract":"The effect of fibrillation earthworm feeding on degradation of dissolved substances has been studied by the intermittent test. As the earthworm density changes, the conversion of certain substances in the activated sludge system, including the sludge activity, ammonia nitrogen and COD treatment effect has been investigated. From the perspective of predation, it may reduce the removal efficiency of system for NH4+-N when insect fibrillation earthworm body present in the reactor because of the feeding effect on nitrifying bacteria. The results show that there is some impact on the degradation rate of ammonia degradation for the presence of fibrillation earthworm, but little for the removal effect of final ammonia and COD, especially the final ammonia. And by measuring the value of OUR, the right flutter earthworm density will play an active role in bacteria in the activated sludge system.","PeriodicalId":6374,"journal":{"name":"2012 International Conference on Biomedical Engineering and Biotechnology","volume":"06 1","pages":"1803-1806"},"PeriodicalIF":0.0,"publicationDate":"2012-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81903789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
An analysis method for the determination of platinum group elements (PGEs) in human hair and urine samples by microwave digestion and inductively coupled plasma mass spectrometry (ICP-MS) was developed. The microwave degestion conditions for different samples were studied in detail. Under the optimal conditions, the detection limits of Pt, Pd and Rh in hair samples were 0.02 ng/g, 0.05 ng/g and 0.002 ng/g, respectively. The recoveries of Pt, Pd and Rh for human hair and urine samples were above 81.7%. The method was applied for determination of PGEs in unrine and hair samples of people with and without car driving experince. The results showed that the concentration of PGEs in human hair was Pd>; Pt>; Rh and increased as the car driving time. However, the concentrations of PGEs in the urine samples were in the extreme low levels and under the detection limits except one sample of a bus driver with 11 years driving time, 0.72ng.g-1 of Pd was found in this case.
{"title":"Determination of Platinum Group Elements in Human Urine and Hair Samples by Inductively Coupled Plasma Mass Spectrometry","authors":"Hong Qiang, Ran Li, Xiaoxia Ju, R. Zhu","doi":"10.1109/ICBEB.2012.134","DOIUrl":"https://doi.org/10.1109/ICBEB.2012.134","url":null,"abstract":"An analysis method for the determination of platinum group elements (PGEs) in human hair and urine samples by microwave digestion and inductively coupled plasma mass spectrometry (ICP-MS) was developed. The microwave degestion conditions for different samples were studied in detail. Under the optimal conditions, the detection limits of Pt, Pd and Rh in hair samples were 0.02 ng/g, 0.05 ng/g and 0.002 ng/g, respectively. The recoveries of Pt, Pd and Rh for human hair and urine samples were above 81.7%. The method was applied for determination of PGEs in unrine and hair samples of people with and without car driving experince. The results showed that the concentration of PGEs in human hair was Pd>; Pt>; Rh and increased as the car driving time. However, the concentrations of PGEs in the urine samples were in the extreme low levels and under the detection limits except one sample of a bus driver with 11 years driving time, 0.72ng.g-1 of Pd was found in this case.","PeriodicalId":6374,"journal":{"name":"2012 International Conference on Biomedical Engineering and Biotechnology","volume":"13 1","pages":"1318-1321"},"PeriodicalIF":0.0,"publicationDate":"2012-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82131558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sonoporation is an important drug/gene delivery technology in drug/gene targeting therapy. In this article, the fibroblast cell and red blood cell suspensions were added contrast agent micro bubbles and sonicated by continues low-intensity ultrasound, and then the pores on cell membrane were detected by scanning electron microscopy. It was found that the sonoporation on cell membrane was positive correlation with the ultrasound exposure power and ultrasound exposure time. And it was found that the sonoporation was related with cell membrane elastic characteristics. It provided experiment basement for the qualitative and quantitative research on sonoporation.
{"title":"Analysis of Sonoporation on Cell Membrane by Scanning Electron Microscopy","authors":"Haojie Yu, Siping Chen","doi":"10.1109/ICBEB.2012.70","DOIUrl":"https://doi.org/10.1109/ICBEB.2012.70","url":null,"abstract":"Sonoporation is an important drug/gene delivery technology in drug/gene targeting therapy. In this article, the fibroblast cell and red blood cell suspensions were added contrast agent micro bubbles and sonicated by continues low-intensity ultrasound, and then the pores on cell membrane were detected by scanning electron microscopy. It was found that the sonoporation on cell membrane was positive correlation with the ultrasound exposure power and ultrasound exposure time. And it was found that the sonoporation was related with cell membrane elastic characteristics. It provided experiment basement for the qualitative and quantitative research on sonoporation.","PeriodicalId":6374,"journal":{"name":"2012 International Conference on Biomedical Engineering and Biotechnology","volume":"117 1","pages":"968-971"},"PeriodicalIF":0.0,"publicationDate":"2012-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79515716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Coherent Anti-Stokes Raman Scattering (CARS) has been an useful method for microscopic analysis of biological molecule, due to its good directionality, the high scattering intensity and the low background noise. In this paper, we review the development of coherent anti-Stokes Raman scattering (CARS) microscopic imaging in the recent years, introduce the application of CARS technology in microscopic imaging theory as well as the suppression of non-resonant background noise in CARS microscopic imaging technology, and show some typical CARS microscopic imaging systems, advantages and fields of CARS microscopic imaging technique are also conclude at the same time.
{"title":"Introduction on Microscopic Imaging Technique Based on CARS","authors":"W. Xiong, Wang Peng","doi":"10.1109/ICBEB.2012.254","DOIUrl":"https://doi.org/10.1109/ICBEB.2012.254","url":null,"abstract":"Coherent Anti-Stokes Raman Scattering (CARS) has been an useful method for microscopic analysis of biological molecule, due to its good directionality, the high scattering intensity and the low background noise. In this paper, we review the development of coherent anti-Stokes Raman scattering (CARS) microscopic imaging in the recent years, introduce the application of CARS technology in microscopic imaging theory as well as the suppression of non-resonant background noise in CARS microscopic imaging technology, and show some typical CARS microscopic imaging systems, advantages and fields of CARS microscopic imaging technique are also conclude at the same time.","PeriodicalId":6374,"journal":{"name":"2012 International Conference on Biomedical Engineering and Biotechnology","volume":"1 1","pages":"829-833"},"PeriodicalIF":0.0,"publicationDate":"2012-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79826348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
GacS/GacA global two-component regulatory systems are comprised of a membrane-bound environmental sensor, GacS, and a transcriptional response regulator, GacA. GacS/ GacA homologs have since been identified in some plant pathogenic bacterial. A current model proposes that GacS senses a still-unknown signal and activates, via a phosphorelay mechanism, the GacA transcription regulator, which in turn triggers the expression of target genes. Certain genes whose expression is regulated by the GacS/GacA system are regulated in parallel by the small RNA binding protein RsmA (CsrA) at a posttranscriptional level. It is suggested that the GacS/GacA system operates a switch between primary and secondary metabolism, with a major involvement of posttranscriptional control mechanisms.
{"title":"Molecular Mechanisms of Two-Component System GacS/GacA in Plant-Pathogenic Bacteria","authors":"Liwei Zhou, Jinzhi Song, B. Wang","doi":"10.1109/ICBEB.2012.477","DOIUrl":"https://doi.org/10.1109/ICBEB.2012.477","url":null,"abstract":"GacS/GacA global two-component regulatory systems are comprised of a membrane-bound environmental sensor, GacS, and a transcriptional response regulator, GacA. GacS/ GacA homologs have since been identified in some plant pathogenic bacterial. A current model proposes that GacS senses a still-unknown signal and activates, via a phosphorelay mechanism, the GacA transcription regulator, which in turn triggers the expression of target genes. Certain genes whose expression is regulated by the GacS/GacA system are regulated in parallel by the small RNA binding protein RsmA (CsrA) at a posttranscriptional level. It is suggested that the GacS/GacA system operates a switch between primary and secondary metabolism, with a major involvement of posttranscriptional control mechanisms.","PeriodicalId":6374,"journal":{"name":"2012 International Conference on Biomedical Engineering and Biotechnology","volume":"2 1","pages":"1066-1069"},"PeriodicalIF":0.0,"publicationDate":"2012-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84881171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
W. Chuang, K. Young, P. Chao, S. Tsai, Shin-Yuan Chen
Deep brain stimulation (DBS) is one of promising therapy to treat advanced Parkinson's disease. During DBS operation, general anesthetic induction has been shown an alternative procedure to avoid patient's discomfort under local anesthesia. Meanwhile, microelectrode recording (MER) is a guide of optimal placement in the brain. However, the inhibition of power of neural spiking is assumed by the general anesthesia during the process. Therefore, we use median nerve stimulation (MNS) to enhance them. At the same time, the power of background noise is also enhanced. Then, it makes MER be poor to determine the optimal electrode placement. In this study, we present a methodology including spike detection, fast Fourier transformation and power spectrum analysis for analyzing MER. Through these results, MER data can be successfully utilized for tracking dorsolateral sub thalamic nucleus (STN) to ensure optimal electrode location.
{"title":"Locating Optimal Electrodes Placement via Microelectrode Recording in General Anesthetic Patients During Deep Brain Stimulation","authors":"W. Chuang, K. Young, P. Chao, S. Tsai, Shin-Yuan Chen","doi":"10.1109/ICBEB.2012.266","DOIUrl":"https://doi.org/10.1109/ICBEB.2012.266","url":null,"abstract":"Deep brain stimulation (DBS) is one of promising therapy to treat advanced Parkinson's disease. During DBS operation, general anesthetic induction has been shown an alternative procedure to avoid patient's discomfort under local anesthesia. Meanwhile, microelectrode recording (MER) is a guide of optimal placement in the brain. However, the inhibition of power of neural spiking is assumed by the general anesthesia during the process. Therefore, we use median nerve stimulation (MNS) to enhance them. At the same time, the power of background noise is also enhanced. Then, it makes MER be poor to determine the optimal electrode placement. In this study, we present a methodology including spike detection, fast Fourier transformation and power spectrum analysis for analyzing MER. Through these results, MER data can be successfully utilized for tracking dorsolateral sub thalamic nucleus (STN) to ensure optimal electrode location.","PeriodicalId":6374,"journal":{"name":"2012 International Conference on Biomedical Engineering and Biotechnology","volume":"92 1","pages":"1048-1051"},"PeriodicalIF":0.0,"publicationDate":"2012-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83795490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The main psychoactive constituent of marijuana, delta-9-trans-tetrahydrocannabinol (Δ9-trans-THC), first elucidated about 40 years ago by Gaoni and Mechoulam, has been shown to possess versatility physiological functions including nervous system bioactivities, immune modulation bioactivities, anticancer bioactivities and so on. This review summarized the total synthesis and biological activities of Δ9-trans-THC.
{"title":"Advances in Studies on Synthesis and Biological Activity of Delta-9-trans-Tetrahydrocannabinol","authors":"Yan-ling Song, Baina Li, Yan-qiu Meng","doi":"10.1109/ICBEB.2012.51","DOIUrl":"https://doi.org/10.1109/ICBEB.2012.51","url":null,"abstract":"The main psychoactive constituent of marijuana, delta-9-trans-tetrahydrocannabinol (Δ9-trans-THC), first elucidated about 40 years ago by Gaoni and Mechoulam, has been shown to possess versatility physiological functions including nervous system bioactivities, immune modulation bioactivities, anticancer bioactivities and so on. This review summarized the total synthesis and biological activities of Δ9-trans-THC.","PeriodicalId":6374,"journal":{"name":"2012 International Conference on Biomedical Engineering and Biotechnology","volume":"99 1","pages":"34-36"},"PeriodicalIF":0.0,"publicationDate":"2012-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82393910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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