Duan Changli, Tian Jun-mei, Wang Shunan, Cao Jie, Chi Xuemei, Guan Yueqing
Doxorubicin (DOX) is a highly active anti-cancer drug for the clinical use, but its therapeutic effectiveness is limited by its severe cardio toxic side effects and dose limiting. In order to over come these limitations, we produced a folate-bovine serum albumin(BSA)-cis-aconitic anhydride-doxorubicin (FA-BSA-CAD) conjugates. Folic acid (FA) is an essential vitamin and has high affinity to folate receptor (FR). FA was bound to BSA for increasing the active targeting of the conjugates. BSA was used as a carrier to provide passive tumor targeting by the enhanced permeability and retention (EPR) effect. DOX is conjugated with BSA with a pH sensitive linker cis-aconitic anhydride (CAA), which can be hydrolyzed in acidic lyzosomal environment. The conjugate was purified and separated by a Sephadex G-25 column. The successful synthesized of FA-BSA-CAD conjugates was further confirmed by mass spectrometry, UV-visible spectroscopy and FTIR. The therapeutical efficacy of the product was evaluated on three different tumor lines (BEL-7402, MDA-MB-231, MCF-7), with normal cell line as control (HELF). Results indicated that FA-BSA-CAD has selectively targeting ability to tumor cell lines, with less toxicity to the normal cells. In addition, the therapeutical efficacy is obvious increased compared to the pure DOX.
{"title":"Synthesis and Evaluation of a Folate-linked Anti-cancer Prodrug","authors":"Duan Changli, Tian Jun-mei, Wang Shunan, Cao Jie, Chi Xuemei, Guan Yueqing","doi":"10.1109/ICBEB.2012.388","DOIUrl":"https://doi.org/10.1109/ICBEB.2012.388","url":null,"abstract":"Doxorubicin (DOX) is a highly active anti-cancer drug for the clinical use, but its therapeutic effectiveness is limited by its severe cardio toxic side effects and dose limiting. In order to over come these limitations, we produced a folate-bovine serum albumin(BSA)-cis-aconitic anhydride-doxorubicin (FA-BSA-CAD) conjugates. Folic acid (FA) is an essential vitamin and has high affinity to folate receptor (FR). FA was bound to BSA for increasing the active targeting of the conjugates. BSA was used as a carrier to provide passive tumor targeting by the enhanced permeability and retention (EPR) effect. DOX is conjugated with BSA with a pH sensitive linker cis-aconitic anhydride (CAA), which can be hydrolyzed in acidic lyzosomal environment. The conjugate was purified and separated by a Sephadex G-25 column. The successful synthesized of FA-BSA-CAD conjugates was further confirmed by mass spectrometry, UV-visible spectroscopy and FTIR. The therapeutical efficacy of the product was evaluated on three different tumor lines (BEL-7402, MDA-MB-231, MCF-7), with normal cell line as control (HELF). Results indicated that FA-BSA-CAD has selectively targeting ability to tumor cell lines, with less toxicity to the normal cells. In addition, the therapeutical efficacy is obvious increased compared to the pure DOX.","PeriodicalId":6374,"journal":{"name":"2012 International Conference on Biomedical Engineering and Biotechnology","volume":"62 1","pages":"1089-1092"},"PeriodicalIF":0.0,"publicationDate":"2012-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89098057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Regular check of blood prostate specific antigen (PSA) level is very important as it is related to prostate cancer. Hence, this study aims to build up a novel impedimetric PSA immumosensor (PSA-IMS) for accurate measurement of PSA. The impedimetric PSA-IMS was fabricated with PSA antibody immobilized on screen-printed carbon electrodes. Impedance measurement at a specific frequency ranges (22.80 Hz - 2.57 kHz) showed that the PSA-IMS has an excellent response range (6.25 - 400 ng/mL), with r2>;0.9, which covers the normal physiological and pathological ranges of blood PSA levels. Intraclass correlation coefficient (ICC) showed that the PSA-IMS has excellent reliability and validit (ICC>;0.95). In conclusion, a simple and reliable PSA-IMS was developed and it is capable of precisely determining blood PSA levels in the range of pathological and normal physiological regions. in both the normal physiological and pathological ranges.
{"title":"Impedimetric PSA Immunosensor: Prostate Specific Antigen Biosensor","authors":"C. Ching, Tzong-Ru Chou, T. Sun, H. Shieh","doi":"10.1109/ICBEB.2012.247","DOIUrl":"https://doi.org/10.1109/ICBEB.2012.247","url":null,"abstract":"Regular check of blood prostate specific antigen (PSA) level is very important as it is related to prostate cancer. Hence, this study aims to build up a novel impedimetric PSA immumosensor (PSA-IMS) for accurate measurement of PSA. The impedimetric PSA-IMS was fabricated with PSA antibody immobilized on screen-printed carbon electrodes. Impedance measurement at a specific frequency ranges (22.80 Hz - 2.57 kHz) showed that the PSA-IMS has an excellent response range (6.25 - 400 ng/mL), with r2>;0.9, which covers the normal physiological and pathological ranges of blood PSA levels. Intraclass correlation coefficient (ICC) showed that the PSA-IMS has excellent reliability and validit (ICC>;0.95). In conclusion, a simple and reliable PSA-IMS was developed and it is capable of precisely determining blood PSA levels in the range of pathological and normal physiological regions. in both the normal physiological and pathological ranges.","PeriodicalId":6374,"journal":{"name":"2012 International Conference on Biomedical Engineering and Biotechnology","volume":"38 1","pages":"329-331"},"PeriodicalIF":0.0,"publicationDate":"2012-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80685435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D. Huang, Wei Liu, Zhaolong Huang, Yashun Chen, Jing Wang, Na Wu
Mathematical mass transfer model of racemic resolution for chiral compounds using HFSLM was presented in this study. Factors such as transportation resistance of boundary layers in strip phase inside the hollow fiber and in feed phase on the shell side of the hollow fiber module, diffusion resistance in the membrane solution and equilibrium constant of interfacial chemical reactions are considered in this mathematical model. The validity of the model is tested by resolution of D, L-phenylalanine with HFSLM using CuN2 as chiral carrier dissolved in a organic solvent (hexanol :decane, 1:1). Using experimental results, several parameters of the proposed model have been achieved by nonlinear fitting method.
本文建立了手性化合物外消旋体分离的数学传质模型。该数学模型考虑了中空纤维内部条相和中空纤维模块壳侧进料相边界层的输运阻力、膜溶液中的扩散阻力和界面化学反应的平衡常数等因素。在有机溶剂(己醇:癸烷，1:1)中，以CuN2为手性载体，用HFSLM分离D, l -苯丙氨酸，验证了模型的有效性。根据实验结果，采用非线性拟合方法得到了该模型的若干参数。
{"title":"Enantioseparation of Enantiomers across Hollow Fiber Supported Liquid Membranes","authors":"D. Huang, Wei Liu, Zhaolong Huang, Yashun Chen, Jing Wang, Na Wu","doi":"10.1109/ICBEB.2012.195","DOIUrl":"https://doi.org/10.1109/ICBEB.2012.195","url":null,"abstract":"Mathematical mass transfer model of racemic resolution for chiral compounds using HFSLM was presented in this study. Factors such as transportation resistance of boundary layers in strip phase inside the hollow fiber and in feed phase on the shell side of the hollow fiber module, diffusion resistance in the membrane solution and equilibrium constant of interfacial chemical reactions are considered in this mathematical model. The validity of the model is tested by resolution of D, L-phenylalanine with HFSLM using CuN2 as chiral carrier dissolved in a organic solvent (hexanol :decane, 1:1). Using experimental results, several parameters of the proposed model have been achieved by nonlinear fitting method.","PeriodicalId":6374,"journal":{"name":"2012 International Conference on Biomedical Engineering and Biotechnology","volume":"18 1","pages":"242-245"},"PeriodicalIF":0.0,"publicationDate":"2012-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83446785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Due to abrupt increase in interacting strength of probed molecules and the AFM tip, the dramatic change in tip vibrations leads to a loss or inadequate acquisition of height information during the scanning across the sample surfaces. Consequently, stripe noises occur and immediately become the first encountered obstacle for characterizing objects in AFM images. The un-supervised DeStripe has been developed for removing stripes based on the Fourier spectrum of image. Since identification of noisy pixels is a critical step in the denoising procedure, the pixels selected by un-supervised DeStripe as potentially noisy may not be satisfactory for the user. The present work presents an alternative that the potentially noisy pixels are decided by input parameters. The computational procedure mainly follows the design of un-supervised DeStripe as described previously [1]. We found that the molecular feature of the cleaned image by the supervised protocol is more prominent than that by the un-supervised DeStripe.
{"title":"Removal of Non-uniform Stripe Noises from AFM Images","authors":"J. Pellequer, Y. Chen, S. W. Chen","doi":"10.1109/ICBEB.2012.335","DOIUrl":"https://doi.org/10.1109/ICBEB.2012.335","url":null,"abstract":"Due to abrupt increase in interacting strength of probed molecules and the AFM tip, the dramatic change in tip vibrations leads to a loss or inadequate acquisition of height information during the scanning across the sample surfaces. Consequently, stripe noises occur and immediately become the first encountered obstacle for characterizing objects in AFM images. The un-supervised DeStripe has been developed for removing stripes based on the Fourier spectrum of image. Since identification of noisy pixels is a critical step in the denoising procedure, the pixels selected by un-supervised DeStripe as potentially noisy may not be satisfactory for the user. The present work presents an alternative that the potentially noisy pixels are decided by input parameters. The computational procedure mainly follows the design of un-supervised DeStripe as described previously [1]. We found that the molecular feature of the cleaned image by the supervised protocol is more prominent than that by the un-supervised DeStripe.","PeriodicalId":6374,"journal":{"name":"2012 International Conference on Biomedical Engineering and Biotechnology","volume":"21 1","pages":"876-879"},"PeriodicalIF":0.0,"publicationDate":"2012-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87298696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Baculovirus has emerged to be a promising gene delivery vector and adipose-derived stem cells have captured interests for bone regeneration. Massive segmental defects arising from trauma or tumor resection remain a challenging clinical problem. To heal massive, segmental bone defects using adipose derived stem cells (ASCs), which alone cannot heal large defects, we hypothesized that sustained expression of factors promoting bone regeneration (BMP2) and angiogenesis (VEGF) provides continuous stimuli to augment the healing. Insect baculovirus (BV) holds promise for gene therapy and efficiently transduces stem cells, but it only mediates transient transgene expression. Therefore we developed a dual BV system whereby one BV expressed FLP recombinase (BacFLP) while the other hybrid BV harbored an Frt-flanking transgene cassette. After sequential transduction with BacFLP and the hybrid BV, FLP/Frt-mediated recombination occurred in up to 46% of ASCs, leading to cassette excision off the BV genome, formation and persistence of episomal transgene and prolongation of expression to >;28 days. Transduction of ASCs with the BMP2-encoding hybrid BV led to prolonged BMP2 expression and augmented ASCs osteogenesis even without other osteogenic supplements. ASCs engineered by the hybrid vectors mediating sustained BMP2/VEGF expression healed the critical-size (10 mm) segmental bone defects in 12 out of 12 New Zealand White rabbits in 8 weeks, which significantly outperformed ASCs engineered with BV transiently expressing BMP2/VEGF with respect to healing rate, bone metabolism, bone volume, bone density, angiogenesis and mechanical properties. These data confirmed our hypothesis that persistent BMP2/VEGF expression is essential. The use of the hybrid BV vector represents a novel therapy to treat massive segmental defects necessitating concerted ossification and vascularization.
{"title":"Baculovirus-Mediated Gene Delivery for Bone Engineering","authors":"Yu-Chen Hu, Chin-Yu Lin","doi":"10.1109/ICBEB.2012.91","DOIUrl":"https://doi.org/10.1109/ICBEB.2012.91","url":null,"abstract":"Baculovirus has emerged to be a promising gene delivery vector and adipose-derived stem cells have captured interests for bone regeneration. Massive segmental defects arising from trauma or tumor resection remain a challenging clinical problem. To heal massive, segmental bone defects using adipose derived stem cells (ASCs), which alone cannot heal large defects, we hypothesized that sustained expression of factors promoting bone regeneration (BMP2) and angiogenesis (VEGF) provides continuous stimuli to augment the healing. Insect baculovirus (BV) holds promise for gene therapy and efficiently transduces stem cells, but it only mediates transient transgene expression. Therefore we developed a dual BV system whereby one BV expressed FLP recombinase (BacFLP) while the other hybrid BV harbored an Frt-flanking transgene cassette. After sequential transduction with BacFLP and the hybrid BV, FLP/Frt-mediated recombination occurred in up to 46% of ASCs, leading to cassette excision off the BV genome, formation and persistence of episomal transgene and prolongation of expression to >;28 days. Transduction of ASCs with the BMP2-encoding hybrid BV led to prolonged BMP2 expression and augmented ASCs osteogenesis even without other osteogenic supplements. ASCs engineered by the hybrid vectors mediating sustained BMP2/VEGF expression healed the critical-size (10 mm) segmental bone defects in 12 out of 12 New Zealand White rabbits in 8 weeks, which significantly outperformed ASCs engineered with BV transiently expressing BMP2/VEGF with respect to healing rate, bone metabolism, bone volume, bone density, angiogenesis and mechanical properties. These data confirmed our hypothesis that persistent BMP2/VEGF expression is essential. The use of the hybrid BV vector represents a novel therapy to treat massive segmental defects necessitating concerted ossification and vascularization.","PeriodicalId":6374,"journal":{"name":"2012 International Conference on Biomedical Engineering and Biotechnology","volume":"24 1","pages":"976-977"},"PeriodicalIF":0.0,"publicationDate":"2012-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87350232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The effect of different mixed fertilizers on the growth and development in Stevia Rebaudiana Bertoni had been studied in the open field. The results showed that the growth rate, plant height, stem thickness, leaf areas and dry matter weight using organic cultivation were less than that of using the chemical fertilizers in the early stage after transplanting. But 60 days after transplanting, there were no significant differences among the treatments in agronomic characters besides the no applying fertilizer treatment. However, there were significant differences in growth and development between organic cultivation and using the chemical fertilizers in 80 days after transplanting.
{"title":"Effect of Different Mixed Fertilizers on the Growth and Development in Stevia Rebaudiana Bertoni","authors":"Liyuan Yan, X. Liu, Yan Shi","doi":"10.1109/ICBEB.2012.478","DOIUrl":"https://doi.org/10.1109/ICBEB.2012.478","url":null,"abstract":"The effect of different mixed fertilizers on the growth and development in Stevia Rebaudiana Bertoni had been studied in the open field. The results showed that the growth rate, plant height, stem thickness, leaf areas and dry matter weight using organic cultivation were less than that of using the chemical fertilizers in the early stage after transplanting. But 60 days after transplanting, there were no significant differences among the treatments in agronomic characters besides the no applying fertilizer treatment. However, there were significant differences in growth and development between organic cultivation and using the chemical fertilizers in 80 days after transplanting.","PeriodicalId":6374,"journal":{"name":"2012 International Conference on Biomedical Engineering and Biotechnology","volume":"1 1","pages":"198-201"},"PeriodicalIF":0.0,"publicationDate":"2012-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84780345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Using tender stems of the current-year in 5-year-old P. elliottii × P. caribaea as explants and exploiting orthogonal experiments, we optimized the growth conditions of Pinus elliottii × Pinus caribaea in vitro and successfully obtained the plantlets. We found that: (1) as explants, fragments at 1.5~3.0 cm from top to bottom of stems that cultured in DCR media supplemented with 2.0 mg/L N6-benzyladenine (6-BA), 0.10 mg/L alpha-naphthyl acetic acid (NAA) and 30 g/L sucrose have the best early differentiation rate of 43.7%; (2) among the tested conditions, DCR media supplemented with 2.5 mg/L 6-BA and 0.2 mg/L NAA is most suitable for propagation of adventitious buds giving a propagation rate and fold of 96.7% and 6.2, respectively, (3) DCR media supplemented with 0.3 mg/L NAA, 1.5 g/L activated carbon and 20 g/L sucrose is best for elongation of adventitious buds giving propagation rate of 444.6%; (4) transplantation of seedlings to 1/2 DCR media supplemented with 2.0 mg/L NAA and cultured in dark for 10 days followed by illumination 12 h/d with 40 μmol·m-2·s-1 intensity resulted as high as 55.3% rooting rate.
{"title":"Stem Tissue Culture of Pinus elliottii × Pinus caribaea","authors":"C. Lv, Baoling Huang","doi":"10.1109/ICBEB.2012.366","DOIUrl":"https://doi.org/10.1109/ICBEB.2012.366","url":null,"abstract":"Using tender stems of the current-year in 5-year-old P. elliottii × P. caribaea as explants and exploiting orthogonal experiments, we optimized the growth conditions of Pinus elliottii × Pinus caribaea in vitro and successfully obtained the plantlets. We found that: (1) as explants, fragments at 1.5~3.0 cm from top to bottom of stems that cultured in DCR media supplemented with 2.0 mg/L N6-benzyladenine (6-BA), 0.10 mg/L alpha-naphthyl acetic acid (NAA) and 30 g/L sucrose have the best early differentiation rate of 43.7%; (2) among the tested conditions, DCR media supplemented with 2.5 mg/L 6-BA and 0.2 mg/L NAA is most suitable for propagation of adventitious buds giving a propagation rate and fold of 96.7% and 6.2, respectively, (3) DCR media supplemented with 0.3 mg/L NAA, 1.5 g/L activated carbon and 20 g/L sucrose is best for elongation of adventitious buds giving propagation rate of 444.6%; (4) transplantation of seedlings to 1/2 DCR media supplemented with 2.0 mg/L NAA and cultured in dark for 10 days followed by illumination 12 h/d with 40 μmol·m-2·s-1 intensity resulted as high as 55.3% rooting rate.","PeriodicalId":6374,"journal":{"name":"2012 International Conference on Biomedical Engineering and Biotechnology","volume":"18 1","pages":"1700-1703"},"PeriodicalIF":0.0,"publicationDate":"2012-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83696520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
We developed a new drug field effect transistor (drug-FET) sensor to detect the quinine concentration in medicinal solution. A drug sensitive membrane, prepared by adding electric active matter into a poly vinyl choride solution, and an ion sensitive field effect transistor were used to compose this sensor. The pH influence, selectivity and response curve of this sensor were investigated. The experimental results indicated that the sensor showed a Nernst response to quinine with a slope of 58.2 mV/decade over the concentration range of 1.0×10-2-3.2×10-5 mol/L and the pH range of 3.0-6.0. Within the detection limit about 1.0×10-6 mol/L, the performance of this sensor was very stable. The obtained results agreed well with the pharmacopoeia method. A preliminary application of the sensor was discussed as well.
{"title":"Determination of Quinine with Phosphotungstic Acid Modified Ion Sensitive Field Effect Transistor Sensor","authors":"Xianwen Li, Hefeng Xu","doi":"10.1109/ICBEB.2012.135","DOIUrl":"https://doi.org/10.1109/ICBEB.2012.135","url":null,"abstract":"We developed a new drug field effect transistor (drug-FET) sensor to detect the quinine concentration in medicinal solution. A drug sensitive membrane, prepared by adding electric active matter into a poly vinyl choride solution, and an ion sensitive field effect transistor were used to compose this sensor. The pH influence, selectivity and response curve of this sensor were investigated. The experimental results indicated that the sensor showed a Nernst response to quinine with a slope of 58.2 mV/decade over the concentration range of 1.0×10-2-3.2×10-5 mol/L and the pH range of 3.0-6.0. Within the detection limit about 1.0×10-6 mol/L, the performance of this sensor was very stable. The obtained results agreed well with the pharmacopoeia method. A preliminary application of the sensor was discussed as well.","PeriodicalId":6374,"journal":{"name":"2012 International Conference on Biomedical Engineering and Biotechnology","volume":"2 1","pages":"772-774"},"PeriodicalIF":0.0,"publicationDate":"2012-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89661555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ya-Yun Cheng, Hsiao-Mei Chang, Hong-Ren Su, S. Lai, Kai-Che Liu, Chien Lin
In this paper, we present a system for segmenting the 3D liver region from CT images and reconstructing its 3D model. The segmentation is accomplished in 3-D space which is extended from the user controlled 2-D random walker technique and implemented by a slice-section method. After obtaining the 3D liver segmentation result, we apply the surface reconstruction algorithm based on bipartite polar classification to build the 3-D liver surface model. In the experimental results, we apply the proposed algorithm to ten test CT datasets to evaluate its accuracy and we demonstrate the good accuracy compared with the human labeled results.
{"title":"3D Liver Segmentation and Model Reconstruction from CT Images","authors":"Ya-Yun Cheng, Hsiao-Mei Chang, Hong-Ren Su, S. Lai, Kai-Che Liu, Chien Lin","doi":"10.1109/ICBEB.2012.18","DOIUrl":"https://doi.org/10.1109/ICBEB.2012.18","url":null,"abstract":"In this paper, we present a system for segmenting the 3D liver region from CT images and reconstructing its 3D model. The segmentation is accomplished in 3-D space which is extended from the user controlled 2-D random walker technique and implemented by a slice-section method. After obtaining the 3D liver segmentation result, we apply the surface reconstruction algorithm based on bipartite polar classification to build the 3-D liver surface model. In the experimental results, we apply the proposed algorithm to ten test CT datasets to evaluate its accuracy and we demonstrate the good accuracy compared with the human labeled results.","PeriodicalId":6374,"journal":{"name":"2012 International Conference on Biomedical Engineering and Biotechnology","volume":"23 1","pages":"654-657"},"PeriodicalIF":0.0,"publicationDate":"2012-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89749115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
To explore the expression changes of trefoil factor 2 (TFF2) and trefoil factor3 (TFF3) gene in submandibular glands during the self healing of experimental gastric ulcer in rats for revealing the relationship between them. The expression of TFF2, TFF3 peptide and genes were detected by immunohistochemistry and RT-PCR methods respectively in gastric ulcer group ,saline control group and normal group. The results were as follows: (1) TFF2 peptide showed strong expression in dark cells with few granules or in those cells without granules, but weakly positive or negative in cells with many granules. The expression was stronger in male rats than that in female rats. The optical density values (OD) values obviously increased on the day 1 after gastric ulcer (P<;0.01), and the lowest on the day 2, then gradually increased again on the day 4, 6 (P<;0.01) , and kept on a high level till 10d to 23d (P<;0.05). Compared with normal group, the OD of TFF2/GAPDH in 1,4,6,10,14 and 23d in gastric ulcer group were obviously higher than that in normal group except the 2d (P<;0.01 or P<;0.05). (2) TFF3 expression was higher in gastric ulcer than that in normal groups on the first day after surgery (P<;0.01), the optical density values (OD) were lower on the 2nd day than that on the 1st day (P<;0.05), then increased gradually on the 4th day and 6th day after gastric ulcer, and reached the highest on the 10th day (P<;0.01), It was still higher on the 23rd than that in normal group (P<;0.05). The OD values of TFF3/GAPDH in gastric ulcer group on the day of 1, 2, 4, 6, 10, 14, 23 are obviously higher than that in normal group (P<;0.01). Rat submaxillary glands TFF2 and TFF3 gene is chiefly in submaxillary ducts of normal and gastric ulcer rat.They may participate in the regulation of self-healing in gastric ulcer with the maner of exocrine.
{"title":"The Changes of Submaxilary Glands Trefoil Factor2 Gene and Trefoil Factor3 Gene during Experimental Gastric Ulcer in Rat","authors":"Jing-Fang Wu, Jing Zhang","doi":"10.1109/ICBEB.2012.401","DOIUrl":"https://doi.org/10.1109/ICBEB.2012.401","url":null,"abstract":"To explore the expression changes of trefoil factor 2 (TFF2) and trefoil factor3 (TFF3) gene in submandibular glands during the self healing of experimental gastric ulcer in rats for revealing the relationship between them. The expression of TFF2, TFF3 peptide and genes were detected by immunohistochemistry and RT-PCR methods respectively in gastric ulcer group ,saline control group and normal group. The results were as follows: (1) TFF2 peptide showed strong expression in dark cells with few granules or in those cells without granules, but weakly positive or negative in cells with many granules. The expression was stronger in male rats than that in female rats. The optical density values (OD) values obviously increased on the day 1 after gastric ulcer (P<;0.01), and the lowest on the day 2, then gradually increased again on the day 4, 6 (P<;0.01) , and kept on a high level till 10d to 23d (P<;0.05). Compared with normal group, the OD of TFF2/GAPDH in 1,4,6,10,14 and 23d in gastric ulcer group were obviously higher than that in normal group except the 2d (P<;0.01 or P<;0.05). (2) TFF3 expression was higher in gastric ulcer than that in normal groups on the first day after surgery (P<;0.01), the optical density values (OD) were lower on the 2nd day than that on the 1st day (P<;0.05), then increased gradually on the 4th day and 6th day after gastric ulcer, and reached the highest on the 10th day (P<;0.01), It was still higher on the 23rd than that in normal group (P<;0.05). The OD values of TFF3/GAPDH in gastric ulcer group on the day of 1, 2, 4, 6, 10, 14, 23 are obviously higher than that in normal group (P<;0.01). Rat submaxillary glands TFF2 and TFF3 gene is chiefly in submaxillary ducts of normal and gastric ulcer rat.They may participate in the regulation of self-healing in gastric ulcer with the maner of exocrine.","PeriodicalId":6374,"journal":{"name":"2012 International Conference on Biomedical Engineering and Biotechnology","volume":"111 1","pages":"1778-1782"},"PeriodicalIF":0.0,"publicationDate":"2012-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84853600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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