Geologic environment quality (GEQ) have a serious influence on engineering construction. So classifying GEQ becomes especially important. In this paper, fuzzy clustering model is used to classify GEQ and classification number affects the results has been analyzed. Combining with an engineering case, the rationality of classification results has been analyzed and satisfactory result has been obtained. At the same time, this paper analyzes application of binary comparison method on clustering classification.
{"title":"Application of Fuzzy Clustering Iterative Model in Classification of Geologic Environment Quality","authors":"X. Yu, Zhongyuan Zhang","doi":"10.1109/ICBEB.2012.80","DOIUrl":"https://doi.org/10.1109/ICBEB.2012.80","url":null,"abstract":"Geologic environment quality (GEQ) have a serious influence on engineering construction. So classifying GEQ becomes especially important. In this paper, fuzzy clustering model is used to classify GEQ and classification number affects the results has been analyzed. Combining with an engineering case, the rationality of classification results has been analyzed and satisfactory result has been obtained. At the same time, this paper analyzes application of binary comparison method on clustering classification.","PeriodicalId":6374,"journal":{"name":"2012 International Conference on Biomedical Engineering and Biotechnology","volume":"31 1","pages":"1224-1227"},"PeriodicalIF":0.0,"publicationDate":"2012-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85124585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Five kinds of TiO2 photo catalysts doped with Fe3+, Ag+, Eu3+, Ni2+, respectively, by Sol-Gel method from Ti(OC4H9)4, were prepared to investigate different UV photo catalytic activity in the Ciba-Red aqueous solution. The effects of pH and the size of titania particles on the photo catalytic degradation rate of Ciba-Red aqueous solution were studied. TiO2 doped with the bimetal ions, Fe3+ and Eu3+, showed best photo catalytic activity than other catalysts. The photocalytic degradation showed a quasi-first-order reaction kinetics. The mechanism of the photo catalytic degradation was also discussed.
{"title":"TiO2 Doped with Metal Ions as the UV Photocatalyts for Ciba-Red Wastewater Treatment","authors":"C. Sun, Xianxiang Sun","doi":"10.1109/ICBEB.2012.450","DOIUrl":"https://doi.org/10.1109/ICBEB.2012.450","url":null,"abstract":"Five kinds of TiO<sub>2</sub> photo catalysts doped with Fe<sup>3+</sup>, Ag<sup>+</sup>, Eu<sup>3+</sup>, Ni<sup>2+</sup>, respectively, by Sol-Gel method from Ti(OC<sub>4</sub>H<sub>9</sub>)<sub>4</sub>, were prepared to investigate different UV photo catalytic activity in the Ciba-Red aqueous solution. The effects of pH and the size of titania particles on the photo catalytic degradation rate of Ciba-Red aqueous solution were studied. TiO<sub>2</sub> doped with the bimetal ions, Fe<sup>3+</sup> and Eu<sup>3+</sup>, showed best photo catalytic activity than other catalysts. The photocalytic degradation showed a quasi-first-order reaction kinetics. The mechanism of the photo catalytic degradation was also discussed.","PeriodicalId":6374,"journal":{"name":"2012 International Conference on Biomedical Engineering and Biotechnology","volume":"6 1","pages":"1858-1861"},"PeriodicalIF":0.0,"publicationDate":"2012-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84281428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Zhisheng Wu, Min Du, Chenglin Sui, Bing Xu, Yanfang Peng, Xinyuan Shi, Yanjiang Qiao
The present study was to develop and validate a hand-held AOTF-NIR measurement method enable to accurately determine low dose of Chinese Herbal Medicines (CHM). Yinhuang oral solutions were taken as an example, and an experimental protocol was then followed, involving two operators and independent production lots for data acquisition. On the basis of this protocol, partial least squares regression (PLS) was then carried out. This result in a calibration with standard errors of calibration (SEC) and determination coefficient (R2) was equal to 146.6 μg/mL and 0.9945. In prediction step, prediction set gave standard errors of prediction (SEP) and R2 of 87.7 μg/mL and 0.9923, respectively. Furthermore, a novel approach based on accuracy profile was used to validate the PLS model. The resulting accuracy profile clearly showed that PLS model was able to determine baicalin content, which the LLOQ was 2020 μg/mL. It was concluded that the hand-held AOTF-NIR measurement method could be used for complicated low content CHM, paving the way for portable environment.
{"title":"Development and Validation of a Portable AOTF-NIR Measurement Method for the Determination of Baicalin in Yinhuang Oral Solution","authors":"Zhisheng Wu, Min Du, Chenglin Sui, Bing Xu, Yanfang Peng, Xinyuan Shi, Yanjiang Qiao","doi":"10.1109/ICBEB.2012.137","DOIUrl":"https://doi.org/10.1109/ICBEB.2012.137","url":null,"abstract":"The present study was to develop and validate a hand-held AOTF-NIR measurement method enable to accurately determine low dose of Chinese Herbal Medicines (CHM). Yinhuang oral solutions were taken as an example, and an experimental protocol was then followed, involving two operators and independent production lots for data acquisition. On the basis of this protocol, partial least squares regression (PLS) was then carried out. This result in a calibration with standard errors of calibration (SEC) and determination coefficient (R2) was equal to 146.6 μg/mL and 0.9945. In prediction step, prediction set gave standard errors of prediction (SEP) and R2 of 87.7 μg/mL and 0.9923, respectively. Furthermore, a novel approach based on accuracy profile was used to validate the PLS model. The resulting accuracy profile clearly showed that PLS model was able to determine baicalin content, which the LLOQ was 2020 μg/mL. It was concluded that the hand-held AOTF-NIR measurement method could be used for complicated low content CHM, paving the way for portable environment.","PeriodicalId":6374,"journal":{"name":"2012 International Conference on Biomedical Engineering and Biotechnology","volume":"121 1","pages":"1322-1326"},"PeriodicalIF":0.0,"publicationDate":"2012-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87985877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Zhou Xiao-zhi, Sang Shuxun, Cao Liwen, Cheng Yunhuan
Surface waste dehydration and settlement process has a direct influence on leachate production and subsequent landfill system stability assessment. In this paper, the author carried out a simulation experiment and studied the effect of initial moisture content, reaction temperature, compacted density and waste granularity on surface waste dehydration and settlement, which provided a theoretical basis for the optimization of traditional landfill structure and operations. The results show that daily evaporation decreases gradually following the piecewise exponential function curve, and reaction temperature has a significant effect on surface waste evaporation. When reaction temperature increased from 25°C to 45°C, cumulative evaporation increased from 162.7g/kg MSW to 220.6g/kg MSW in the simulation experiment. Initial moisture content significantly affects leachate production of surface waste, and leachate output increased with moisture content increasing Reaction temperature had a significant influence on moisture loss, and it increases with reaction temperature. Compacted density significantly effects the settlement of landfill body. With the increase of compacted density, accumulative settlement decreases rapidly.
{"title":"Simulation Study on the Dehydration and Settlement Characteristics of Surface Waste in Landfill","authors":"Zhou Xiao-zhi, Sang Shuxun, Cao Liwen, Cheng Yunhuan","doi":"10.1109/ICBEB.2012.355","DOIUrl":"https://doi.org/10.1109/ICBEB.2012.355","url":null,"abstract":"Surface waste dehydration and settlement process has a direct influence on leachate production and subsequent landfill system stability assessment. In this paper, the author carried out a simulation experiment and studied the effect of initial moisture content, reaction temperature, compacted density and waste granularity on surface waste dehydration and settlement, which provided a theoretical basis for the optimization of traditional landfill structure and operations. The results show that daily evaporation decreases gradually following the piecewise exponential function curve, and reaction temperature has a significant effect on surface waste evaporation. When reaction temperature increased from 25°C to 45°C, cumulative evaporation increased from 162.7g/kg MSW to 220.6g/kg MSW in the simulation experiment. Initial moisture content significantly affects leachate production of surface waste, and leachate output increased with moisture content increasing Reaction temperature had a significant influence on moisture loss, and it increases with reaction temperature. Compacted density significantly effects the settlement of landfill body. With the increase of compacted density, accumulative settlement decreases rapidly.","PeriodicalId":6374,"journal":{"name":"2012 International Conference on Biomedical Engineering and Biotechnology","volume":"198 1","pages":"515-518"},"PeriodicalIF":0.0,"publicationDate":"2012-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88305132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fouling exists in almost every circulating cooling water system. It greatly harms to the operation of the system. According to their formation, fouling can be classified into crystallization fouling, particulate fouling, chemical reaction fouling, corrosion fouling, biological fouling, condensation fouling and mixed fouling. There are many factors influence fouling, and the main factors include the fluid temperature, flow rate, heat-transfer surface temperature and so on. The formation of fouling involves origin, transport, adhesion, exfoliation and aging stage. Due to difference of the formation mechanism and applicable conditions, fouling models are also different. Two typical models were introduced in the paper.
{"title":"Fouling in Circulating Cooling Water System","authors":"Ping Xu, Yajun Zhang, Xiao-dong Liu, Ting Liu","doi":"10.1109/ICBEB.2012.228","DOIUrl":"https://doi.org/10.1109/ICBEB.2012.228","url":null,"abstract":"Fouling exists in almost every circulating cooling water system. It greatly harms to the operation of the system. According to their formation, fouling can be classified into crystallization fouling, particulate fouling, chemical reaction fouling, corrosion fouling, biological fouling, condensation fouling and mixed fouling. There are many factors influence fouling, and the main factors include the fluid temperature, flow rate, heat-transfer surface temperature and so on. The formation of fouling involves origin, transport, adhesion, exfoliation and aging stage. Due to difference of the formation mechanism and applicable conditions, fouling models are also different. Two typical models were introduced in the paper.","PeriodicalId":6374,"journal":{"name":"2012 International Conference on Biomedical Engineering and Biotechnology","volume":"8 1","pages":"1486-1489"},"PeriodicalIF":0.0,"publicationDate":"2012-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86876384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The paper proposes a compressive sensing method adapted to ultrasound imaging, following the recently developed Analog to Information (AIC) framework. First, basis functions in time domain based on waveform were constructed to achieve sparse signal representation. Second, utilizing the basis functions and using AIC framework, a system is designed with lowered the sampling rates and reduced the total data size of ultrasound imaging. The results show that in the experiment the sampling rate below Nyquist frequency and only 30% amount of data are used to implement ultrasound imaging without reducing the quality of image. The sampling rate is lowered and the amount of data is reduced greatly by the proposed method based on compressive sensing.
{"title":"Compressive Ultrasound Imaging Based on Analog to Information Conversion","authors":"Yi Lv, Wen-tao Wu","doi":"10.1109/ICBEB.2012.110","DOIUrl":"https://doi.org/10.1109/ICBEB.2012.110","url":null,"abstract":"The paper proposes a compressive sensing method adapted to ultrasound imaging, following the recently developed Analog to Information (AIC) framework. First, basis functions in time domain based on waveform were constructed to achieve sparse signal representation. Second, utilizing the basis functions and using AIC framework, a system is designed with lowered the sampling rates and reduced the total data size of ultrasound imaging. The results show that in the experiment the sampling rate below Nyquist frequency and only 30% amount of data are used to implement ultrasound imaging without reducing the quality of image. The sampling rate is lowered and the amount of data is reduced greatly by the proposed method based on compressive sensing.","PeriodicalId":6374,"journal":{"name":"2012 International Conference on Biomedical Engineering and Biotechnology","volume":"18 1","pages":"758-761"},"PeriodicalIF":0.0,"publicationDate":"2012-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85388006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yan Bai, Xing Wang, J. Lei, C. Xie, Qiujuan Ma, Juan Zhang, Yongsheng Ji
In this paper, near-infrared spectroscopy (NIRS) combined with discriminant analysis was used as a qualitative tool to distinguish Fructus forsythiae from different geographical origins by collecting and analysing the NIR spectra in the range of 12000-4000cm-1. NIR spectra were analyzed by two methods: clustering analysis and discriminant analysis. In the former, the second derivative and Norris smoothing were utilized together to correct the scattering effect and eliminate the baseline shift in the range 4092--8008 cm-1 in order to achieve a reliable classification on Fructus forsythiae from different geographical origins. The latter method was used to establish a discriminant analysis model which can be used to identify the geographical origin of Fructus forsythiae. The result shows that the correct rate of the internal cross-validation identification is 96.99%. By the model, 12 samples were analyzed, and the prognostications of their geographical origins are exact on the whole. It's indicated that NIR along with the chemo metrics methods provides a simple and accurate way in rapid dertimination of herbal medicines from different geographical origins.
{"title":"Discrimination of Fructus forsythiae According to Geographical Origin with Near-infared Spectroscopy","authors":"Yan Bai, Xing Wang, J. Lei, C. Xie, Qiujuan Ma, Juan Zhang, Yongsheng Ji","doi":"10.1109/ICBEB.2012.150","DOIUrl":"https://doi.org/10.1109/ICBEB.2012.150","url":null,"abstract":"In this paper, near-infrared spectroscopy (NIRS) combined with discriminant analysis was used as a qualitative tool to distinguish Fructus forsythiae from different geographical origins by collecting and analysing the NIR spectra in the range of 12000-4000cm-1. NIR spectra were analyzed by two methods: clustering analysis and discriminant analysis. In the former, the second derivative and Norris smoothing were utilized together to correct the scattering effect and eliminate the baseline shift in the range 4092--8008 cm-1 in order to achieve a reliable classification on Fructus forsythiae from different geographical origins. The latter method was used to establish a discriminant analysis model which can be used to identify the geographical origin of Fructus forsythiae. The result shows that the correct rate of the internal cross-validation identification is 96.99%. By the model, 12 samples were analyzed, and the prognostications of their geographical origins are exact on the whole. It's indicated that NIR along with the chemo metrics methods provides a simple and accurate way in rapid dertimination of herbal medicines from different geographical origins.","PeriodicalId":6374,"journal":{"name":"2012 International Conference on Biomedical Engineering and Biotechnology","volume":"9 1","pages":"175-178"},"PeriodicalIF":0.0,"publicationDate":"2012-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82248109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
As the member of receptor tyrosine kinase family, epidermal growth factor receptor (EGFR) is closely related to the growth of tumor. Thus, it has become a significant and potential target molecule for the screening of anti-tumor drug. In this study, a new immobilized EGFR micro-reactor was established for fast screening EGFR inhibitors. Enzyme was immobilized into the inner wall of capillary with three chemical modification methods. Combined with capillary electrophoresis technique, the addressable immobilized EGFR micro-reactor could easily separate and capture EGFR inhibitors from the complex mixtures with high selectivity. The performance of the micro-reactor was studied with Gefitinib, which showed good recognization ability. The influence on stability and activity of the immobilized enzyme prepared by three methods was further investigated. It was demonstrated that the layer-by-layer assembling method was the optimized one, which showed both good stability and activity. It is expected that this screening platform would be widely used in the discovery of the new protein inhibitors as a convenient and universal method.
{"title":"Preparation of Immobilized EGFR Micro-reactor Based on CE","authors":"Gang-yi Shen, Wanting Yu, Lingpeng Pei, Xun Cui","doi":"10.1109/ICBEB.2012.309","DOIUrl":"https://doi.org/10.1109/ICBEB.2012.309","url":null,"abstract":"As the member of receptor tyrosine kinase family, epidermal growth factor receptor (EGFR) is closely related to the growth of tumor. Thus, it has become a significant and potential target molecule for the screening of anti-tumor drug. In this study, a new immobilized EGFR micro-reactor was established for fast screening EGFR inhibitors. Enzyme was immobilized into the inner wall of capillary with three chemical modification methods. Combined with capillary electrophoresis technique, the addressable immobilized EGFR micro-reactor could easily separate and capture EGFR inhibitors from the complex mixtures with high selectivity. The performance of the micro-reactor was studied with Gefitinib, which showed good recognization ability. The influence on stability and activity of the immobilized enzyme prepared by three methods was further investigated. It was demonstrated that the layer-by-layer assembling method was the optimized one, which showed both good stability and activity. It is expected that this screening platform would be widely used in the discovery of the new protein inhibitors as a convenient and universal method.","PeriodicalId":6374,"journal":{"name":"2012 International Conference on Biomedical Engineering and Biotechnology","volume":"42 4 1","pages":"1606-1609"},"PeriodicalIF":0.0,"publicationDate":"2012-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81165798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Zi Yang, Xiaohui Zhou, Jianjun Song, Xuexing Fang, X. Xiong
The CpxA/R signal transduction system is one prevalent two-component systems (TCSs) in gram-negative bacteria involved in the process of bacterial adherence, invasion, motility and cell division. CpxP is the auxiliary inhibitor of Cpx-two-component system in Escherichia Coli. However, the functions of Cpx pathway in gram-negative plant pathogenic bacteria are still unknown. Previous results showed that cpxP deleted S. typhimurium is more invasive, therefore CpxP was spectulated as an inhibitor of Cpx-signalling-cascade in plant pathogenic bacteria and inhibiting its invasion to the host. To obtain the structural information of CpxP in plant pathogenic bacteria for its pathogenic research, here, for the first time, the cpxP gene from Pectobacterium carotovorum subsp. carotovorum (PC1), a plant pathogenic bacterium, was cloned into pET15b plasmid for further expression in BL21 (DE3) strain. In this study, the vector with cpxP gene was constructed which provides a way to express and purify the CpxP protein for further structural and functional studies from plant pathogenic bacteria.
{"title":"Construction and Expression of CpxP Protein from Plant Pathogenic Bacteria","authors":"Zi Yang, Xiaohui Zhou, Jianjun Song, Xuexing Fang, X. Xiong","doi":"10.1109/ICBEB.2012.112","DOIUrl":"https://doi.org/10.1109/ICBEB.2012.112","url":null,"abstract":"The CpxA/R signal transduction system is one prevalent two-component systems (TCSs) in gram-negative bacteria involved in the process of bacterial adherence, invasion, motility and cell division. CpxP is the auxiliary inhibitor of Cpx-two-component system in Escherichia Coli. However, the functions of Cpx pathway in gram-negative plant pathogenic bacteria are still unknown. Previous results showed that cpxP deleted S. typhimurium is more invasive, therefore CpxP was spectulated as an inhibitor of Cpx-signalling-cascade in plant pathogenic bacteria and inhibiting its invasion to the host. To obtain the structural information of CpxP in plant pathogenic bacteria for its pathogenic research, here, for the first time, the cpxP gene from Pectobacterium carotovorum subsp. carotovorum (PC1), a plant pathogenic bacterium, was cloned into pET15b plasmid for further expression in BL21 (DE3) strain. In this study, the vector with cpxP gene was constructed which provides a way to express and purify the CpxP protein for further structural and functional studies from plant pathogenic bacteria.","PeriodicalId":6374,"journal":{"name":"2012 International Conference on Biomedical Engineering and Biotechnology","volume":"4 6 1","pages":"119-122"},"PeriodicalIF":0.0,"publicationDate":"2012-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82840210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
To develop the reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) method for the detection of European brown hare syndrome virus (EBHSV), a set of 6 primers were designed according to the conserved sequence of the capsid protein(VP60)gene of EBHSV published in GenBank, and the RT-LAMP assay was described through synthesis of target DNA fragments, optimization of reaction conditions, sensitivity and specificity tests. Results showed the RT-LAMP method for the EBHSV detection had a ladder-like pattern of amplication bands from about 233 bp incubation at 64°C for 1h by using agarose gel electrophoresis, and with good specificity and sensitivity. The sensitivity of the RT-LAMP could reach about 5 copies of cloned viral genomic fragments (pGM-T-EBHSV), and no amplifications for RHDV, Pasteur Ella multocida, E.coli and Salmonella from rabbits detection by this approach.
{"title":"Primary Study on Rapid Detection of European Brown Hare Syndrome Virus by RT-LAMP","authors":"Zexiao Yang, Xianchao Wei, Yin Wang, X. Yao, Kaiyu Wang, Ling Zhu, Xue-qing Han, Ze-Xiao Yang, Huaiping Shi, Yanming Zhang","doi":"10.1109/ICBEB.2012.313","DOIUrl":"https://doi.org/10.1109/ICBEB.2012.313","url":null,"abstract":"To develop the reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) method for the detection of European brown hare syndrome virus (EBHSV), a set of 6 primers were designed according to the conserved sequence of the capsid protein(VP60)gene of EBHSV published in GenBank, and the RT-LAMP assay was described through synthesis of target DNA fragments, optimization of reaction conditions, sensitivity and specificity tests. Results showed the RT-LAMP method for the EBHSV detection had a ladder-like pattern of amplication bands from about 233 bp incubation at 64°C for 1h by using agarose gel electrophoresis, and with good specificity and sensitivity. The sensitivity of the RT-LAMP could reach about 5 copies of cloned viral genomic fragments (pGM-T-EBHSV), and no amplifications for RHDV, Pasteur Ella multocida, E.coli and Salmonella from rabbits detection by this approach.","PeriodicalId":6374,"journal":{"name":"2012 International Conference on Biomedical Engineering and Biotechnology","volume":"97 2 1","pages":"441-445"},"PeriodicalIF":0.0,"publicationDate":"2012-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82574780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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