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2012 International Conference on Biomedical Engineering and Biotechnology最新文献

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An Unbiased Tissue Attenuation Coefficient Estimator Using Microbubble Echoes with Harmonic Ratio Compensation 带谐波比补偿的微泡回波无偏组织衰减系数估计
Pub Date : 2012-05-28 DOI: 10.1109/ICBEB.2012.64
Sheng-Kai Tsao, J. Tsao
For the tissue attenuation coefficient (TAC) estimator proposed in our previous paper [5], to have unbiased estimate of the TAC of separation tissue between two vessels, it needs bubbles in these two vessels have identical statistical properties, which can be achieved only when these two vessels have same diameter and blood pressure. To release this limitation, a compensation technique is proposed to suppress the bias. In this paper, follow Greenleaf's work, we introduce a parameter named harmonic ratio (HR), which is the ratio of two second-harmonic signals, for bias suppression. This method needs to use dual-frequency excitation to produce two harmonics. After applying HR to estimate TAC, it is shown that the effect of bubble number can be compensated. In the experimental work, we make two vessels with different bubble concentrations such that the statistical properties of bubble echoes are different to cause severe bias on TAC estimates, then it is shown that the estimates using HR can be kept unbiased.
对于我们在之前的论文[5]中提出的组织衰减系数(tissue attenuation coefficient, TAC)估计器,要对两根血管间分离组织的TAC进行无偏估计,需要两根血管中的气泡具有相同的统计性质,而这只有在两根血管的直径和血压相同的情况下才能实现。为了消除这一限制,提出了一种补偿技术来抑制偏置。本文在Greenleaf的基础上,引入谐波比(HR)参数,即两个二次谐波信号的比值,用于抑制偏置。这种方法需要使用双频激励产生两个谐波。应用HR估计TAC后,表明气泡数的影响是可以补偿的。在实验工作中,我们制作了两个气泡浓度不同的容器,使得气泡回波的统计特性不同,对TAC估计造成了严重的偏差,然后证明了使用HR估计可以保持无偏。
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引用次数: 1
Assessing Cancer Risk of Coal-Fired Power Plant Workers Exposed to PAHs 评估燃煤电厂工人接触多环芳烃的癌症风险
Pub Date : 2012-05-28 DOI: 10.1109/ICBEB.2012.82
Bin Li, Zhaolong Zhang, H. Fan, C. Zeng
To study the relationship between the concentration of urinary 1-OH-Py, 3-OH-BaP and the degree as well as the pathways of human exposure to PAHs, we collected 24-hour air, dietary and urine samples of 60 oven workers in a coal-fired power plant of Central China and 60 people in a general group not exposed to such PAHs in 2011. The concentrations of eight strong carcinogenic PAHs in air and dietary samples, and the concentrations of the urinary 1-OH-Py and 3-OH-BaP were determined. By studying the pathways of human exposure to PAHs, it is found that the main reason for coal-fired power plant workers' high cancer risk is the exposure to PAHs by breathing contaminated air, while the degree of exposure to PAHs through diet is similar to that of the general group. In order to reduce coal-fired power plant workers' cancer risk, occupational protection should be strengthened, and the working environment should be improved.
为了研究尿中1-OH-Py、3-OH-BaP浓度与人体多环芳烃暴露程度及途径的关系,我们于2011年收集了华中某燃煤电厂60名烤炉工人和60名未接触多环芳烃的普通人群24小时的空气、饮食和尿液样本。测定了空气和饮食样品中8种强致癌性多环芳烃的浓度,以及尿液中1-OH-Py和3-OH-BaP的浓度。通过对人体接触多环芳烃途径的研究,发现燃煤电厂工人的高致癌风险主要是通过呼吸污染的空气接触到多环芳烃,而通过饮食接触多环芳烃的程度与一般人群相似。为降低燃煤电厂工人患癌风险,应加强职业防护,改善工作环境。
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引用次数: 0
Study of Simulation on Attenuation of Diesel Oil during Remediation of Contaminated Soil by Enhancing Bioventing 加强生物通风修复污染土壤过程中柴油衰减模拟研究
Pub Date : 2012-05-28 DOI: 10.1109/ICBEB.2012.373
Jin-Feng Yang, Zhao Tong-ke, Zhang Cheng-jun, Cheng Hong-han
Bioventing is an in situ forced oxidative soil remediation technology which combined soil vapor extraction with biodegradation. It has broad application prospects of soil contamination caused by underground storage tank leakage. In this paper, soil column experiment which was used to simulate diesel contamination was conducted. The law of balance distribution of residual TPH (Total Petroleum Hydrocarbon) concentration at different time and the changes of diesel amount in soil at different depths, together with total diesel amount of the entire column were analyzed. The results showed that: (1) The initial soil moisture content affects the distribution and changes of TPH content during the natural attenuation and enhancing bioventing. (2) Gravity diffusion and migration was the main causes for the changes of TPH content of entire column in the early days of natural attenuation, within one month. Approximately 2 months' venting later, extraction was prominent to maintain stable changes of the diesel concentration in the upper of soil column.
生物通风是一种将土壤蒸汽提取与生物降解相结合的原位强制氧化土壤修复技术。地下储罐泄漏引起的土壤污染具有广阔的应用前景。本文进行了模拟柴油机污染的土柱试验。分析了不同时间土壤中残余石油烃(TPH)浓度的平衡分布规律,以及不同深度土壤中柴油含量的变化,以及整个塔的总柴油含量。结果表明:(1)土壤初始含水量影响自然衰减和生物通气增强过程中TPH含量的分布和变化。(2)在自然衰减初期(1个月内),重力扩散和运移是导致全柱TPH含量变化的主要原因。排气约2个月后,提取作用突出,保持了土壤柱上部柴油浓度的稳定变化。
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引用次数: 0
Experimental Study on the Steady Flow in the Carotid Siphon: The Geometric Effect on the Hemodynamics 颈动脉虹吸稳定血流的实验研究:几何效应对血流动力学的影响
Pub Date : 2012-05-28 DOI: 10.1109/ICBEB.2012.204
Chi Zhang, Shuyu Li, F. Pu, Xiaoyan Deng, Yubo Fan, Deyu Li, S. Xie
The siphon bend of internal carotid artery (ICA) has been reported as the most preferred location for stenoses. The geometry of the siphon is considered to be associated with the atherosclerotic stenos is. In the present study, the shape of ICA was classified into 3 types with different curvature and planarity. Accordingly, 3 siphon models were experimentally studied with particle image velocimetry technology. The velocity field of the experimental fluid in the models was measured to simulate the steady blood flow in the ICA siphon. The planarity was considered as an important geometric factor for stenoses, because the helical flow was found when the model has large non-planarity. The experimental measurement was validated with the computationally simulation. More geometric studies are necessary to elucidate the geometric effect on the hemodynamics and stenoses in the ICA siphon in the future.
虹吸弯的颈内动脉(ICA)已被报道为最首选的位置狭窄。虹吸管的几何形状被认为与动脉粥样硬化性狭窄有关。本研究将ICA的形状分为3种不同曲率和平面度的形状。据此,采用粒子图像测速技术对3种虹吸模型进行了实验研究。测量了模型中实验流体的速度场,模拟了ICA虹吸管内的稳定血流。当模型具有较大的非平面度时,会产生螺旋流,因此平面度被认为是影响狭窄的重要几何因素。通过计算仿真验证了实验测量结果。未来需要更多的几何研究来阐明几何对ICA虹吸管血流动力学和狭窄的影响。
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引用次数: 0
Pharmacophore Model Generation of P2Y12 Inhibitor P2Y12抑制剂药效团模型的生成
Pub Date : 2012-05-28 DOI: 10.1109/ICBEB.2012.293
Zhengqiang Yang, Yan-ling Zhang, Xing Wang, Yanjiang Qiao
A three dimensional pharmacophore model was generated for the molecules which are responsible for anti-platelet aggregation activities targeting platelet adp receptor (P2Y12). 24 structrurally diverse molecules were selected as training set to generate the hypothesis using Catalyst software 4.11. The best hypothesis comprises one hydrogen-bond acceptor, one aromatic ring, three hydrophobic points and one excluded volume and shows high correlation coefficient (0.999) as well as low RMS deviation (1.24). It has been further validated towards a test set and shows high correlation coefficient of test set (0.978). The values of effectively active hit A% and comprehensive evaluation index CAI are respectively 40% and 2.795. The results show that the pharmacophore we built is reliable and can be used to screen database. Furthermore, the best hypothesis was used to screen TCMD (Version 2005) database and the four hit compounds of higher predicted activity were the reported anti-platelet aggregation inhibitions, which may be useful for further study.
建立了针对血小板adp受体(P2Y12)的抗血小板聚集活性分子的三维药效团模型。选取24个结构多样的分子作为训练集,使用Catalyst软件4.11生成假设。最佳假设包含1个氢键受体、1个芳环、3个疏水点和1个排除体积,相关系数高(0.999),均方根偏差低(1.24)。对测试集进行了进一步验证,测试集的相关系数较高(0.978)。有效主动命中A%为40%,综合评价指标CAI为2.795。结果表明所建立的药效团是可靠的,可用于筛选数据库。此外,利用最佳假设筛选TCMD (Version 2005)数据库,发现预测活性较高的4种hit化合物均为已报道的抗血小板聚集抑制剂,为进一步研究提供参考。
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引用次数: 5
Heavy Metals in Water, Soil and Aquatic Plants from a Rice-Crab Production Base of the Liao River Delta 辽河三角洲稻蟹生产基地水体、土壤及水生植物重金属含量分析
Pub Date : 2012-05-28 DOI: 10.1109/ICBEB.2012.237
Xiaoyun Chen, Fuyu Sun, Wentao Sun, F. Yu, Zhiqiang Li
In order to reveal the transformation of heavy metals in rice-crab ecosystems and to maintain the sustainable development of crab industry, it is important for evaluating the environmental quality of rice-crab production base of the Liao River Delta. Therefore, the contents of heavy metals in water, soil and aquatic plants of the rice-crab production base in the Liao River Delta were monitored from 2008 to 2010. The results showed that the contents of total Pb, Cd, Hg and As in the water and soil of the rice-crab production base were very low and qualified to the requirements for the green food crab. However, the contents of total Pb in some aquatic plants, such as Monochoria trifolia, Ceratophyllum demersum, Elodea Canadensis and Potamogeton distinct us, exceeded the limits of Pollution-free Food Standard for Freshwater Crab (NY 5072-2002). In conclusion, as the feed of crabs, these aquatic plants could affect the quality safety for raising rice-crab in the Liao River Delta, Northeast China.
为了揭示稻蟹生态系统中重金属的转化,维护蟹业的可持续发展,对辽河三角洲稻蟹生产基地进行环境质量评价具有重要意义。为此,2008 - 2010年对辽河三角洲稻蟹生产基地水体、土壤和水生植物重金属含量进行了监测。结果表明,稻蟹生产基地的水体和土壤中总Pb、Cd、Hg和As含量很低,符合绿色食品蟹的要求。然而,在一些水生植物中,如三叶单毛蟹(Monochoria trifolia)、长尾角藻(Ceratophyllum demersum)、加拿大野蟹(Elodea canada)和大闸蟹(Potamogeton distinct us)的总铅含量超过了《淡水蟹无公害食品标准》(NY 5072-2002)的限制。综上所述,这些水生植物作为蟹类的饲料,对辽河三角洲地区稻蟹养殖的质量安全有一定影响。
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引用次数: 1
Impact of Regular Physical Activity on Patterns of Age-Related Changes in Photoplethysmogram Appearance Obtained at Fingertip 有规律的体育活动对指尖光体积描记图外观年龄相关变化模式的影响
Pub Date : 2012-05-28 DOI: 10.1109/ICBEB.2012.245
Lei Wang, Jingjing Zou, Xiaomin Luo
Objective To validate the association between age and photoplethysmogram appearance, explore the impact of regular physical activity to pulse shape pattern and quantify the effect on trend of relative changes with advancing age. Methods This experiment involved 231 Chinese participants (aged 21-60 years, mean 40.10 ± 11.61 years), and their photoplethysmography signals were recorded from left index finger in rest state. The categorical analysis of IPAQ was performed to classify the subjects into levels of regular physical activity. Results Significant correlation coefficients were observed positively for age and Aging Character Index, which supported the previously reported view of association between age and changes in pulse shape characteristics. Significant differences of Aging Character Index between levels of regular physical activity were observed and quantitatively analyzed in all age groups. Conclusion The impact of regular physical activity to aging effect can be quantitatively measured through biological marker derived from photoplethysmogram appearance for dose-response analysis.
目的验证年龄与光容积脉搏图表现的相关性,探讨规律运动对脉搏形态的影响,量化脉搏形态随年龄增长的相对变化趋势。方法231名年龄21 ~ 60岁(平均40.10±11.61岁)的中国受试者在静息状态下用左手食指记录其光体积脉搏波信号。对IPAQ进行分类分析,将受试者按规律体力活动的水平进行分类。结果年龄与衰老特征指数呈显著正相关,支持了先前报道的年龄与脉搏形状特征变化相关的观点。观察并定量分析各年龄组正常体力活动水平之间的衰老特征指数的显著差异。结论定期运动对衰老效应的影响可通过光体积描记图上的生物标记物进行定量测量,并进行剂量效应分析。
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引用次数: 1
Expression of Alpha-smooth Muscle Action in Goat Temporomandibular Joint Disc and Its Effect on Self-Assembled Engineered Constructs 山羊颞下颌关节盘α -平滑肌活动的表达及其对自组装工程结构的影响
Pub Date : 2012-05-28 DOI: 10.1109/ICBEB.2012.211
Hong Kang, Mengyi Qi, G. Bao
Purpose of this study was to investigate the expression of smooth muscle action-alpha (α-SMA) in native goat temporomandibular joint (TMJ) disc and in self-assembled construct of TMJ disc. Expression of a-SMA in native one-month-aged goat TMJ disc was examined by immunohistochemistry (IHC) method. TMJ disc cells were isolated from one-month-aged goat TMJ discs with 0.1% type I collagenase and passaged to P3 for tissue engineering. 5.5×10(6) cells were seeded into 5 mm diameter wells filled with 2% agarose gels in which 10μg/L IGF-I growth factor was added to stimulate mature process of self-assembled constructs of TMJ disc. Dimensions, forms and weights of constructs were measured respectively at the second, fourth, sixth and eighth week. IHC staining was used to examine the distribution of α-SMA in self-assembled constructs. The IHC staining for α-SMA was globally uniformly distributed in all four groups of self-assembled constructs and expressed the strongest in the second group. This experiment also confirmed that much more significant expression of α-SMA in self-assembled constructs than in the native TMJ disc tissue. Conclusively, α-SMA could take part in the regulating developmental process of engineered construct and may be used partially to explain the reason why TMJ disc self-assembled constructs become much smaller and denser during its growth and development due to its volume shrinkage.
本研究旨在探讨平滑肌动作α (α-SMA)在山羊颞下颌关节(TMJ)椎间盘及自组装结构中的表达。采用免疫组化(IHC)方法检测1月龄山羊TMJ椎间盘中a-SMA的表达。用0.1% I型胶原酶从1月龄山羊TMJ椎间盘中分离TMJ椎间盘细胞,传代至P3进行组织工程。5.5×10(6)将细胞接种于直径为5 mm的孔中,孔中填充2%琼脂糖凝胶,加入10μg/L的IGF-I生长因子,刺激TMJ椎间盘自组装结构的成熟过程。分别于第2、4、6、8周测量构念的尺寸、形态和重量。采用免疫组化染色检测α-SMA在自组装构建体中的分布。α-SMA的免疫组化染色在四组自组装构建体中呈全局均匀分布,其中第二组表达最强。本实验还证实,α-SMA在自组装构建体中的表达远高于在天然TMJ椎间盘组织中的表达。综上所述,α-SMA可能参与了工程构建体的发育调控过程,并可能部分解释了TMJ椎间盘自组装构建体在生长发育过程中由于体积收缩而变得更小、密度更大的原因。
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引用次数: 1
Function and Localization Analysis of Zebrafish Rspo1 斑马鱼Rspo1基因的功能及定位分析
Pub Date : 2012-05-28 DOI: 10.1109/ICBEB.2012.231
Dongchang Sun, Yanmei Zhang
R-spondin family factors, known as regulators of β-catenin signaling pathway, are conserved in vertebrate. They play roles in multiple processes during vertebrate development. Rspo1, a member of R-spondin family, regulates sex differentiation in both mammals (e.g. mouse) and reptiles (e.g. turtle). However, the role of Rspo1 in fish remains elusive. Our previous work showed the expression of Rspo1 in zebrafish (zRspo1). In this study, we further investigated potential function of zRspo1. We compared Rspo1 homologs in zebrafish and in human (hRspo1-4). By bioinformatics analysis, we predicted that zRspo1 functions on activating β-catenin signaling through a manner similar to hRSpo1. By histag indication, we detected zRspo1 on cell membrane and Golgi apparatus in zebrafish liver cells. This result suggests that zRspo1 may first secrete to the milieu and then extracellular zRspo1 activates β-catenin.
R-spondin家族因子作为β-catenin信号通路的调节因子,在脊椎动物中是保守的。它们在脊椎动物发育的多个过程中发挥作用。Rspo1是R-spondin家族的一员,在哺乳动物(如小鼠)和爬行动物(如乌龟)中都能调节性别分化。然而,Rspo1在鱼类中的作用仍然难以捉摸。我们之前的工作显示了Rspo1在斑马鱼中的表达(zRspo1)。在本研究中,我们进一步研究了zRspo1的电位功能。我们比较了斑马鱼和人类的Rspo1同源物(hRspo1-4)。通过生物信息学分析,我们预测zRspo1通过类似于hRSpo1的方式激活β-catenin信号。通过组织学指示,我们在斑马鱼肝细胞的细胞膜和高尔基体上检测到zRspo1。这表明zRspo1可能首先分泌到环境中,然后胞外zRspo1激活β-catenin。
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引用次数: 0
Preparation of a P-rich Soil Amendment and Its Effect on the Pot Growth of White Mustard 富磷土壤改良剂的制备及其对白芥菜盆栽生长的影响
Pub Date : 2012-05-28 DOI: 10.1109/ICBEB.2012.305
Yongli Li, Lin Shi
A pot growing test was conducted to evaluate the amelioration effect of the P-rich soil amendment on acid soil and pot growth effect of the white mustard. The P-rich soil amendment was prepared by calcinating a mixture of low-grade rock phosphate and dolomite with a fusing agent of CaSO4. The amendment was rich in P, Si, Ca, Mg and S nutrient elements and displayed an alkalescent. The contents of nutrient elements in the soil amendment dissolved in 0.1 mol·L-1 citric acid solution were 8.94% P2O5, 19.82% CaO, 3.97% MgO, 13.37% SiO2 and 0.86% SO42-. Its pH value was 9.16. In the pot growing, the soil amendment was applied to the pot at four application rates, 1 g/Kg(L1), 2 g/Kg(L2), 3 g/Kg(L3), 4 g/Kg(L4) with 7 treatments and control (CK). The results of white mustard growth for 2 months indicated that applying the P-rich soil amendment could effectively increase the seeding emergence, the whole plant height, the stem thickness, the root length, the dry and wet biomass. The optimized application rate of the P-rich soil amendment was 3g·kg-1. The growth rate of biomass (wet weight) reached 13.52%~115.80%. The experimental results also suggested that the P-rich soil amendment could increase the biomass of white mustard and ameliorate the acid soil, and improve the content of the available phosphorus in the soil.
通过盆栽试验,评价了富磷土壤改良剂对酸性土壤的改良效果和白芥菜的盆栽效果。用CaSO4熔剂煅烧低品位磷矿与白云石的混合物制备富磷土壤改良剂。该改进剂富含P、Si、Ca、Mg、S等营养元素,呈碱性。土壤改良剂在0.1 mol·L-1柠檬酸溶液中溶解的营养元素含量分别为:P2O5 8.94%、CaO 19.82%、MgO 3.97%、SiO2 13.37%、SO42- 0.86%。pH值为9.16。在盆栽条件下,土壤改良剂按1 g/Kg(L1)、2 g/Kg(L2)、3 g/Kg(L3)、4 g/Kg(L4) 4种施用量在盆内施用,7个处理和对照(CK)。2个月的白芥菜生长结果表明,施用富磷土壤改良剂能有效提高白芥菜的出苗率、株高、茎粗、根长以及干、湿生物量。富磷土壤改良剂的最佳施用量为3g·kg-1。生物量(湿重)增长率达到13.52%~115.80%。富磷土壤改良剂可以增加白芥菜生物量,改善酸性土壤,提高土壤速效磷含量。
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引用次数: 0
期刊
2012 International Conference on Biomedical Engineering and Biotechnology
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