Ren Jian-min, Ran Xin-Min, Yang Yang, Yu Zhao-Quan
Transformation between Surface water and Ground water affords possibility for Utilization Ratio of water resources in the Shiyang River Region. Upstream the blind expansion irrigate area, causes the water resources over development utilization. The study indicated that, the only irrigated area amounted to 2.1 hectares to 1989, caused to enter the downriver the river runoff failure even to block the flow, Middle and lower reaches Shiyang River the year ultra picks the quantity to above reach 5×108 m3, only the Minqin Basin year ultra picks the quantity to above reach 4×108 m3, the downriver ground water hardness index by every year 0.1g/L speed growth, the downriver had the serious ecology problem.
{"title":"Transformation between Surface Water and Groundwater under the Influence Development of Agriculture in Arid Region of Northwest China","authors":"Ren Jian-min, Ran Xin-Min, Yang Yang, Yu Zhao-Quan","doi":"10.1109/ICBEB.2012.453","DOIUrl":"https://doi.org/10.1109/ICBEB.2012.453","url":null,"abstract":"Transformation between Surface water and Ground water affords possibility for Utilization Ratio of water resources in the Shiyang River Region. Upstream the blind expansion irrigate area, causes the water resources over development utilization. The study indicated that, the only irrigated area amounted to 2.1 hectares to 1989, caused to enter the downriver the river runoff failure even to block the flow, Middle and lower reaches Shiyang River the year ultra picks the quantity to above reach 5×108 m3, only the Minqin Basin year ultra picks the quantity to above reach 4×108 m3, the downriver ground water hardness index by every year 0.1g/L speed growth, the downriver had the serious ecology problem.","PeriodicalId":6374,"journal":{"name":"2012 International Conference on Biomedical Engineering and Biotechnology","volume":"1 1","pages":"1862-1865"},"PeriodicalIF":0.0,"publicationDate":"2012-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89343464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Plateau pica (Ochotona curzoniae) is the keystone species on Qinghai-Tibet Plateau. We studied relative densities of the pica using pica holes in roadside habitats in 15 locations along the parallel Qinghai-Tibet Railway and Highway by strip transect method in summer 2009. Five transects (3m*150 m) were censused according to the distance levels from the two roads (D=0 m, 300 m, 600 m, 900 m, 1200 m) in each location. Snow finches which were previously considered as neighbors of the pica living in the same hole were also counted in each transect. Our results showed that no differences in density of pica holes were found among the five distances, but there was a positive relationship between pica holes and snow finches. Our results suggested the Qinghai-Tibet Railway and Highway have no effects on the plateau picas.
高原异食兽(Ochotona curzoniae)是青藏高原的重要物种。2009年夏季，采用条带样带法在青藏铁路和公路沿线15个地点的路边生境中设置异食虫孔，研究了异食虫的相对密度。根据每个地点与两条道路的距离水平(D=0 m, 300 m, 600 m, 900 m, 1200 m)，调查了5个3m*150 m的样条。雪雀以前被认为是异食鸟的邻居，生活在同一个洞里，在每个样带中也被计算在内。结果表明，不同距离间异食虫孔密度无显著差异，但异食虫孔与雪雀密度呈正相关。结果表明，青藏铁路和公路对高原空气污染没有影响。
{"title":"Effects of Qinghai-Tibet Railway and Highway on Plateau Picas","authors":"Zhongqiu Li","doi":"10.1109/ICBEB.2012.189","DOIUrl":"https://doi.org/10.1109/ICBEB.2012.189","url":null,"abstract":"Plateau pica (Ochotona curzoniae) is the keystone species on Qinghai-Tibet Plateau. We studied relative densities of the pica using pica holes in roadside habitats in 15 locations along the parallel Qinghai-Tibet Railway and Highway by strip transect method in summer 2009. Five transects (3m*150 m) were censused according to the distance levels from the two roads (D=0 m, 300 m, 600 m, 900 m, 1200 m) in each location. Snow finches which were previously considered as neighbors of the pica living in the same hole were also counted in each transect. Our results showed that no differences in density of pica holes were found among the five distances, but there was a positive relationship between pica holes and snow finches. Our results suggested the Qinghai-Tibet Railway and Highway have no effects on the plateau picas.","PeriodicalId":6374,"journal":{"name":"2012 International Conference on Biomedical Engineering and Biotechnology","volume":"58 1","pages":"224-227"},"PeriodicalIF":0.0,"publicationDate":"2012-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89872881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Qinhong Cai, T. Ng, K. Wong, P. Wong, Y. Xiong, Yonghua Wang, Ying Zhang
A novel method, which determines the concentrations of speciated Cr(III) and Cr(VI) with the presence of Cr(III)-organic complexes (particularly chromium dyes), was developed based on a column solid phase extraction using Amberlite XAD-4 resin (XAD-4). Acid Yellow 99 (AY99), a chromium monoazo dye, used as a model organic compound, could be quantitatively recovered at pH 5 with low recoveries of Cr(III) and Cr(VI) (below 5%). After Cr(VI) being converted to Cr(III)-diphenylcarbazone (Cr-DPC) by 1, 5-diphenylcarbazide, Cr(VI) was quantitatively recovered at pH 1 with the recovery of Cr(III) below 5%. The recoveries of AY99, Cr(III) and Cr(VI) by the newly developed method were 94.7±1.7, 103.0±2.5 and 96.7±3.6%, respectively. In addition, the recoveries of organics (i.e. proteins, amino acids, and organic acids) complexing with chromium ion were all below 5% at pH 1 and had little interference to the selective determination of Cr(VI) concentration. The adsorption capacity and detection limit of XAD-4 for Cr(VI) was 281.6±2.8 μg g-1 and 1.37 μg L-1, respectively.
{"title":"Speciation of Cr(III) and Cr(VI) in the Presence of Chromium Azo Dye Acid Yellow 99 by Column Solid Phase Extraction","authors":"Qinhong Cai, T. Ng, K. Wong, P. Wong, Y. Xiong, Yonghua Wang, Ying Zhang","doi":"10.1109/ICBEB.2012.360","DOIUrl":"https://doi.org/10.1109/ICBEB.2012.360","url":null,"abstract":"A novel method, which determines the concentrations of speciated Cr(III) and Cr(VI) with the presence of Cr(III)-organic complexes (particularly chromium dyes), was developed based on a column solid phase extraction using Amberlite XAD-4 resin (XAD-4). Acid Yellow 99 (AY99), a chromium monoazo dye, used as a model organic compound, could be quantitatively recovered at pH 5 with low recoveries of Cr(III) and Cr(VI) (below 5%). After Cr(VI) being converted to Cr(III)-diphenylcarbazone (Cr-DPC) by 1, 5-diphenylcarbazide, Cr(VI) was quantitatively recovered at pH 1 with the recovery of Cr(III) below 5%. The recoveries of AY99, Cr(III) and Cr(VI) by the newly developed method were 94.7±1.7, 103.0±2.5 and 96.7±3.6%, respectively. In addition, the recoveries of organics (i.e. proteins, amino acids, and organic acids) complexing with chromium ion were all below 5% at pH 1 and had little interference to the selective determination of Cr(VI) concentration. The adsorption capacity and detection limit of XAD-4 for Cr(VI) was 281.6±2.8 μg g-1 and 1.37 μg L-1, respectively.","PeriodicalId":6374,"journal":{"name":"2012 International Conference on Biomedical Engineering and Biotechnology","volume":"19 1","pages":"1684-1687"},"PeriodicalIF":0.0,"publicationDate":"2012-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89967633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Utilizing dental CAD/CAM system to restore occlusal teeth surface provides us with the possibility of greatly reducing the time to serve patients. Surfaces designed should match the existing jaw articulation and preserve the anatomical characteristics of the generic teeth. In addition, to be suitable for clinical applications, the design process must be highly efficient. In this paper, we describe an interactive framework based on Laplacian coordinate translation-invariant to edit the dental surface freely in a user-specific region. The deformation operation can be accomplished with a little user interaction. First, a region of interest(ROI) is fixed, and a handle (composed by some points in ROI) is chosen, then drag the handle freely, the whole ROI is deformed simultaneously. The error engendered by operating the handle is gradually diffused to the whole dental surface, which enables dental surface to be naturally smooth and continuous. The main computation in this framework is to solve a sparse linear system. By employing the most advanced solvers, our framework performs efficiently to guarantee interactive response time. Experimental results show that the presented algorithm is effective. Several examples are given to demonstrate that the deformation operation changes the shape of the models but preserving the geometrically fine details well.
{"title":"An Interactive Deforming Framework for Dental Surface Restoration","authors":"Xin Ma, Mingqiang Wei, Sen Wang, Jianhuang Wu","doi":"10.1109/ICBEB.2012.61","DOIUrl":"https://doi.org/10.1109/ICBEB.2012.61","url":null,"abstract":"Utilizing dental CAD/CAM system to restore occlusal teeth surface provides us with the possibility of greatly reducing the time to serve patients. Surfaces designed should match the existing jaw articulation and preserve the anatomical characteristics of the generic teeth. In addition, to be suitable for clinical applications, the design process must be highly efficient. In this paper, we describe an interactive framework based on Laplacian coordinate translation-invariant to edit the dental surface freely in a user-specific region. The deformation operation can be accomplished with a little user interaction. First, a region of interest(ROI) is fixed, and a handle (composed by some points in ROI) is chosen, then drag the handle freely, the whole ROI is deformed simultaneously. The error engendered by operating the handle is gradually diffused to the whole dental surface, which enables dental surface to be naturally smooth and continuous. The main computation in this framework is to solve a sparse linear system. By employing the most advanced solvers, our framework performs efficiently to guarantee interactive response time. Experimental results show that the presented algorithm is effective. Several examples are given to demonstrate that the deformation operation changes the shape of the models but preserving the geometrically fine details well.","PeriodicalId":6374,"journal":{"name":"2012 International Conference on Biomedical Engineering and Biotechnology","volume":"1 1","pages":"41-44"},"PeriodicalIF":0.0,"publicationDate":"2012-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87892610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Five lactobacillus strains which were obtained from traditional fermented foods in western China, were examined for use as probiotics and for anticancer biological products. The cell walls and cytoplasm extracts from lactobacillus strains were tested for anti-proliferative activity and induction of apoptosis. The results showed the cell walls showed significant direct anticancer activity. 16S rRNA gene sequences of probiotic strains indicated that M5 and X12 were L. paracasei subp. Paracasei, K11 and K14 were Lactobacillus fermentum, X11 was Lactobacillus casei strain respectively. After exposure to the colon cancer cells for 72 h, strong anti-proliferative activity of cell walls from M5, K11 and K14 was efficiently induced through S-phase accumulation in HT-29 cells. Together, these results suggested the cell walls from M5, K11 and K14 to be good probiotic candidates and had potential application in functional products.
{"title":"Effects of Lactobacillus Strains on Colon Cancer Cell Proliferation and Cell Cycle Blockage","authors":"Shumei Wang, Lanwei Zhang, W. Gu","doi":"10.1109/ICBEB.2012.183","DOIUrl":"https://doi.org/10.1109/ICBEB.2012.183","url":null,"abstract":"Five lactobacillus strains which were obtained from traditional fermented foods in western China, were examined for use as probiotics and for anticancer biological products. The cell walls and cytoplasm extracts from lactobacillus strains were tested for anti-proliferative activity and induction of apoptosis. The results showed the cell walls showed significant direct anticancer activity. 16S rRNA gene sequences of probiotic strains indicated that M5 and X12 were L. paracasei subp. Paracasei, K11 and K14 were Lactobacillus fermentum, X11 was Lactobacillus casei strain respectively. After exposure to the colon cancer cells for 72 h, strong anti-proliferative activity of cell walls from M5, K11 and K14 was efficiently induced through S-phase accumulation in HT-29 cells. Together, these results suggested the cell walls from M5, K11 and K14 to be good probiotic candidates and had potential application in functional products.","PeriodicalId":6374,"journal":{"name":"2012 International Conference on Biomedical Engineering and Biotechnology","volume":"95 1","pages":"1015-1018"},"PeriodicalIF":0.0,"publicationDate":"2012-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86599878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
An efficient yeast cell biotransformation process was set up for asymmetric synthesis of (R)-(-)-mandelic acid methyl ester, a key drug intermediate. Saccharomyces cerevisiae 21 was selected as optimum strain for biotransformation. The optimum reduction conditions are as follows: substrate concentration 22 g/L, cell concentration 150 g/L, reaction time 36 h, 30°C, pH5.0. Conversion and enantiometric excess of (R)-(-)-mandelic acid methyl ester reached 99.4% and 99.9%.
{"title":"Synthesis of (R)-(-)-Mandelic Acid Methyl Ester by Asymmetric Reduction of Methyl Benzoylformate with Yeast Cells","authors":"Ou Zhimin, Liu Yong, Nan Yinkang","doi":"10.1109/ICBEB.2012.390","DOIUrl":"https://doi.org/10.1109/ICBEB.2012.390","url":null,"abstract":"An efficient yeast cell biotransformation process was set up for asymmetric synthesis of (R)-(-)-mandelic acid methyl ester, a key drug intermediate. Saccharomyces cerevisiae 21 was selected as optimum strain for biotransformation. The optimum reduction conditions are as follows: substrate concentration 22 g/L, cell concentration 150 g/L, reaction time 36 h, 30°C, pH5.0. Conversion and enantiometric excess of (R)-(-)-mandelic acid methyl ester reached 99.4% and 99.9%.","PeriodicalId":6374,"journal":{"name":"2012 International Conference on Biomedical Engineering and Biotechnology","volume":"45 1","pages":"562-564"},"PeriodicalIF":0.0,"publicationDate":"2012-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86887329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Eimeria ten Ella is a major cause of coccidiosis in chickens, which can lead to serious economic impact on poultry production worldwide. Developing a cost effective sub-unit vaccine may be helpful to decrease loss in the poultry enterprise. Here, we describe expressing etMIC4, which is one of the microneme proteins. It is a 218 kDa microneme protein from Eimeria ten Ella. In this study, RNA was extracted from E. ten Ella sporulated oocysts and N-(etMIC4-N) and C terminal (etMIC4-C) genes were cloned by PCR, according to published etMIC4 cDNA sequence. Blast analysis showed that 773bp of 5 'end and 883bp of 3' end respectively shared more than 98% identy with etMIC4. At last, etMIC4-N was successfully expressed in pichia pastor is and identified by Western Blot, which set up a platform for further research.
艾美耳球虫是引起鸡球虫病的主要原因，可对世界范围内的家禽生产造成严重的经济影响。开发具有成本效益的亚单位疫苗可能有助于减少家禽企业的损失。在这里，我们描述了表达etMIC4，这是微素蛋白之一。它是一个218 kDa的微素蛋白，来自艾美耳球虫。本研究从E. ten Ella孢子卵囊中提取RNA，根据已发表的etMIC4 cDNA序列，采用PCR方法克隆N-(etMIC4-N)和C端(etMIC4-C)基因。Blast分析显示，5′端773bp和3′端883bp分别与etMIC4具有98%以上的同源性。最后，etMIC4-N在毕赤酵母中成功表达，并经Western Blot鉴定，为进一步研究奠定了基础。
{"title":"Expession of Microneme Protein 4 from Eimeria tenella in the Methylotrophic Yeast Pichia pastoris","authors":"Zhou Sai, An Jian, Zhang Peng, W. Lixia","doi":"10.1109/ICBEB.2012.205","DOIUrl":"https://doi.org/10.1109/ICBEB.2012.205","url":null,"abstract":"Eimeria ten Ella is a major cause of coccidiosis in chickens, which can lead to serious economic impact on poultry production worldwide. Developing a cost effective sub-unit vaccine may be helpful to decrease loss in the poultry enterprise. Here, we describe expressing etMIC4, which is one of the microneme proteins. It is a 218 kDa microneme protein from Eimeria ten Ella. In this study, RNA was extracted from E. ten Ella sporulated oocysts and N-(etMIC4-N) and C terminal (etMIC4-C) genes were cloned by PCR, according to published etMIC4 cDNA sequence. Blast analysis showed that 773bp of 5 'end and 883bp of 3' end respectively shared more than 98% identy with etMIC4. At last, etMIC4-N was successfully expressed in pichia pastor is and identified by Western Blot, which set up a platform for further research.","PeriodicalId":6374,"journal":{"name":"2012 International Conference on Biomedical Engineering and Biotechnology","volume":"47 1","pages":"1022-1025"},"PeriodicalIF":0.0,"publicationDate":"2012-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86379759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Meihua Fan, Z. Liao, Li Chen, Ge Shi, Jian Xin Wang
The effects of rare earth elements lanthanum on growth and antioxidation system of the greengrocery under salt stress were investigated. The seed of greengrocery were cultureed in Hoagland nutrient solution with 50mg·L-1 NaCl and 20, 40, 60, 80, 100, 150mg·L-1 Lanthanum. Then the treatments cultured in Hoagland nutrient solution with 50mg·L-1 NaCl, 20mg·L-1 lanthanum as control. After continued for two weeks, seed germination rate and super oxide dismutase(SOD) and peroxidase ( POD) activities, malondialdehyde (MDA) were measured. The results showed that salt stress could inhibite seed germination and seedling growth of greengrocery, fresh weight marked reduced, dwarf seedlings. Low concentration LaCl3(20~80mg·L-1)could promote seed germination and seedling growth of greengrocery, fresh weight marked increased, high seedlings. Salt stress significantly reduced the chlorophyll content of and adding suitable concentration LaCl3 (20~100mg·L-1 )could improve of chlorophyll content of the greengrocery seedlings under salt stress and enhance its light transformation ability. But under hoagland nutrient solution with 20mg·L-1 LaCl3 processing, chlorophyll content of the greengrocery seedlings had marked reduced(P<;0.05 ). Salt stress can induce the generation of protein. The treaments with proper density LaCl3 (40~80mg·L-1) were able to alleviate the pressure of salt stress, and improve the soluble protein content of greengrocery seedling. High concentration LaCl3(100~150mg·L-1) under salt stress marked inhibited the the soluble. protein produce of greengrocery seedling. Salt stress could stimulate greengrocery seedlings induction their antioxidant enzymes protection system, make antioxidant enzymes of greengrocery seedling increase, MDA content reduced, Suitable concentration LaCl3(40-80mg·L-1) under salt stress can promote antioxidant enzymes relieve the damage of salt stress on seedling, then MDA content reduced. but high levels of LaCl3 (100~150mg·L-1)is likely to produce poisonous effect on cells leaded to antioxidant enzymes activity abate, MDA content increased. In brief, salt stress(50mg·L-1) could inhibited seed germination and seedling growth of greengrocery, and induction antioxidant enzymes emerge, MDA content increased. The treatment with optimum concentration of LaCl3 could significantly increase antioxidant enzyme activities of greengrocery seedlings, enhance its scavenging reactive oxygen species, reduce lipid per oxidation under salt stress, in order to effectively alleviate the salt stress injury and increase their salt tolerance, and promote greengrocery seedling growth, The optium concentration was 40~80mg·L-1.
{"title":"Effects of Rare Earth Elements Lanthanum on Growth and Antioxidation System of the Greengrocery under Salt Stress","authors":"Meihua Fan, Z. Liao, Li Chen, Ge Shi, Jian Xin Wang","doi":"10.1109/ICBEB.2012.190","DOIUrl":"https://doi.org/10.1109/ICBEB.2012.190","url":null,"abstract":"The effects of rare earth elements lanthanum on growth and antioxidation system of the greengrocery under salt stress were investigated. The seed of greengrocery were cultureed in Hoagland nutrient solution with 50mg·L<sup>-1</sup> NaCl and 20, 40, 60, 80, 100, 150mg·L<sup>-1</sup> Lanthanum. Then the treatments cultured in Hoagland nutrient solution with 50mg·L<sup>-1</sup> NaCl, 20mg·L<sup>-1</sup> lanthanum as control. After continued for two weeks, seed germination rate and super oxide dismutase(SOD) and peroxidase ( POD) activities, malondialdehyde (MDA) were measured. The results showed that salt stress could inhibite seed germination and seedling growth of greengrocery, fresh weight marked reduced, dwarf seedlings. Low concentration LaCl<sub>3</sub>(20~80mg·L<sup>-1</sup>)could promote seed germination and seedling growth of greengrocery, fresh weight marked increased, high seedlings. Salt stress significantly reduced the chlorophyll content of and adding suitable concentration LaCl<sub>3</sub> (20~100mg·L<sup>-1</sup> )could improve of chlorophyll content of the greengrocery seedlings under salt stress and enhance its light transformation ability. But under hoagland nutrient solution with 20mg·L<sup>-1</sup> LaCl<sub>3</sub> processing, chlorophyll content of the greengrocery seedlings had marked reduced(P<;0.05 ). Salt stress can induce the generation of protein. The treaments with proper density LaCl<sub>3</sub> (40~80mg·L<sup>-1</sup>) were able to alleviate the pressure of salt stress, and improve the soluble protein content of greengrocery seedling. High concentration LaCl<sub>3</sub>(100~150mg·L<sup>-1</sup>) under salt stress marked inhibited the the soluble. protein produce of greengrocery seedling. Salt stress could stimulate greengrocery seedlings induction their antioxidant enzymes protection system, make antioxidant enzymes of greengrocery seedling increase, MDA content reduced, Suitable concentration LaCl<sub>3</sub>(40-80mg·L<sup>-1</sup>) under salt stress can promote antioxidant enzymes relieve the damage of salt stress on seedling, then MDA content reduced. but high levels of LaCl<sub>3</sub> (100~150mg·L<sup>-1</sup>)is likely to produce poisonous effect on cells leaded to antioxidant enzymes activity abate, MDA content increased. In brief, salt stress(50mg·L<sup>-1</sup>) could inhibited seed germination and seedling growth of greengrocery, and induction antioxidant enzymes emerge, MDA content increased. The treatment with optimum concentration of LaCl<sub>3</sub> could significantly increase antioxidant enzyme activities of greengrocery seedlings, enhance its scavenging reactive oxygen species, reduce lipid per oxidation under salt stress, in order to effectively alleviate the salt stress injury and increase their salt tolerance, and promote greengrocery seedling growth, The optium concentration was 40~80mg·L<sup>-1</sup>.","PeriodicalId":6374,"journal":{"name":"2012 International Conference on Biomedical Engineering and Biotechnology","volume":"2016 1","pages":"228-235"},"PeriodicalIF":0.0,"publicationDate":"2012-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86390840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A simple, rapid and reproducible HPLC method was used for the simultaneous determination of chlorogenic acid, baicalin and forsythin in Xiao'erjiebiao Granules, at the same time, response surface methodology (RSM) was devoted to forecast the best extraction technology. The HPLC System included an Agilent ZORBAX SB-C18 column ( 250 mm × 4.6 mm, 5μm ), acetonitrile (A) - 0.4% ethylic acid (B) as mobile phase with gradient elution at a flow rate of 1.0 mL.min-1 and detection wavelength of 327 nm for chlorogenic acid, 280 nm for baicalin and forsythin, respectively. The good linearity was achieved successfully with R2 <; 0.9995 in its standard curve aspects. Recoveries measured at the same concentrations (n = 6) were in the range of 96.4-97.6%. The established assay is accurate and practical, which could be utilized as a quality evaluation and control for Xiao'erjie biao Granules.
采用简便、快速、重现性好的高效液相色谱法同时测定消二解表颗粒中绿原酸、黄芩苷和连翘苷的含量，并采用响应面法预测最佳提取工艺。高效液相色谱系统采用Agilent ZORBAX SB-C18色谱柱(250 mm × 4.6 mm, 5μm)，乙腈(A) - 0.4%乙酸(B)为流动相，梯度洗脱，流速1.0 mL.min-1，绿原酸检测波长327 nm，黄芩苷检测波长280 nm，连素检测波长280 nm。在R2 <;在标准曲线方面为0.9995。在相同浓度(n = 6)下，加样回收率为96.4 ~ 97.6%。所建立的方法准确、实用，可作为消儿解标颗粒的质量评价和控制方法。
{"title":"A Novel HPLC Method for Determining Three Effective Components in Chinese Medicine 'Xiao' Erjiebiao Granules","authors":"Guangcai Qi, Ajing Liu, Z. Liu, Zijin Bai","doi":"10.1109/ICBEB.2012.34","DOIUrl":"https://doi.org/10.1109/ICBEB.2012.34","url":null,"abstract":"A simple, rapid and reproducible HPLC method was used for the simultaneous determination of chlorogenic acid, baicalin and forsythin in Xiao'erjiebiao Granules, at the same time, response surface methodology (RSM) was devoted to forecast the best extraction technology. The HPLC System included an Agilent ZORBAX SB-C18 column ( 250 mm × 4.6 mm, 5μm ), acetonitrile (A) - 0.4% ethylic acid (B) as mobile phase with gradient elution at a flow rate of 1.0 mL.min-1 and detection wavelength of 327 nm for chlorogenic acid, 280 nm for baicalin and forsythin, respectively. The good linearity was achieved successfully with R2 <; 0.9995 in its standard curve aspects. Recoveries measured at the same concentrations (n = 6) were in the range of 96.4-97.6%. The established assay is accurate and practical, which could be utilized as a quality evaluation and control for Xiao'erjie biao Granules.","PeriodicalId":6374,"journal":{"name":"2012 International Conference on Biomedical Engineering and Biotechnology","volume":"26 1","pages":"16-20"},"PeriodicalIF":0.0,"publicationDate":"2012-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88550450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Located in the inland arid regions of northwest China, Malian River Basin suffers an intensive conflict of supply and demand of water resources. And it encounters an even worse situation because of its brackish water which affects the utilization of the water. The feasibility of desalination of the brackish water is analyzed and then a new harnessing strategy is proposed, based on a longtime monitoring of the water quality of the river as well as a reference to previous treatment schemes. Firstly, the brackish water in non-flood period is supposed to be intercepted by the regulation and storage engineering, second, the brackish water will be desalted, and last, the concentrated water will be disposed in an effective, environment-friendly method. Factors of resources, technology, economy and safety have been taken into account in the new strategy. In this way, the brackish water of upstream could be treated, and the middle and lower reach could be used more efficiently. Analysis of the potential of the new harnessing strategy promises an ultimate solution to the brackish water problem of Malian river, and consequently a good utilization rate of the river basin.
{"title":"Study on Harnessing Strategy of Brackish Water in the Malian River","authors":"Lijun Yu, Hao Wang, D. Qin, Yong Zhao, Xuefeng Sang, Hengzheng Xu","doi":"10.1109/ICBEB.2012.376","DOIUrl":"https://doi.org/10.1109/ICBEB.2012.376","url":null,"abstract":"Located in the inland arid regions of northwest China, Malian River Basin suffers an intensive conflict of supply and demand of water resources. And it encounters an even worse situation because of its brackish water which affects the utilization of the water. The feasibility of desalination of the brackish water is analyzed and then a new harnessing strategy is proposed, based on a longtime monitoring of the water quality of the river as well as a reference to previous treatment schemes. Firstly, the brackish water in non-flood period is supposed to be intercepted by the regulation and storage engineering, second, the brackish water will be desalted, and last, the concentrated water will be disposed in an effective, environment-friendly method. Factors of resources, technology, economy and safety have been taken into account in the new strategy. In this way, the brackish water of upstream could be treated, and the middle and lower reach could be used more efficiently. Analysis of the potential of the new harnessing strategy promises an ultimate solution to the brackish water problem of Malian river, and consequently a good utilization rate of the river basin.","PeriodicalId":6374,"journal":{"name":"2012 International Conference on Biomedical Engineering and Biotechnology","volume":"92 1","pages":"1726-1729"},"PeriodicalIF":0.0,"publicationDate":"2012-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79984529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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