High-fidelity simulation of incompressible turbulent channel flows at high Reynolds numbers remains computationally intensive due to their multi-scale nature, necessitating efficient strategies to reconstruct high-resolution (HR) fields from low-resolution (LR) data. This study introduces a physics-guided neural network (PGNN) framework that leverages the elliptic nature of the pressure field in incompressible flows to enhance super-resolution (SR) reconstruction of turbulent velocity fields. Inspired by the global correlation-capturing capabilities of attention mechanisms, we incorporate LR pressure data as auxiliary input features to guide neural networks in inferring local velocity components, thereby explicitly encoding physical constraints into the learning process. High-fidelity training datasets are generated via large-eddy simulations, with LR fields derived through spatial filtering using down-sampling ratios (DSR) of 4, 8. A physics-guided U-Net (pgU-Net) is developed, contrasting with a pure data-driven U-Net baseline. Results demonstrate that integrating pressure fields significantly improves reconstruction accuracy, particularly under challenging DSR of 8, the pgU-Net achieves an R2 score of 0.8048, outperforming the data-driven model (0.6792) and bi-cubic interpolation (−0.1801). By leveraging pressure fields as physical knowledge encoding global flow correlations, our framework effectively addresses the multi-scale reconstruction challenges in turbulent flows while maintaining computational efficiency. By embedding elliptic pressure correlations as physical priors, this framework pioneers a hybrid approach that bridges data-driven learning and fluid physics, offering a robust approach to reduce computational costs while enhancing the fidelity of turbulent flow predictions.