Pub Date : 2009-10-30DOI: 10.1109/BMEI.2009.5305790
Liguo Deng, Zong Min Ma, Gang Zhang
This paper present a fuzzy temporal data model for uncertainty/implicit events more precisely, and introduce fuzzy temporal complex event value relations, which combine the concept of fuzzy temporal relations with the idea of fuzzy temporal values in a uniform framework. We elaborate a model theoretic definition of fuzzy temporal information combination strategies, which has a rigorous foundation on fuzzy temporal theory. And then define an algebra for querying database instances. We prove that our data model and algebra for fuzzy temporal values generalizes the classical relational data model and algebra. Moreover, we show that under certain assumptions, all our algebraic operations are tractable. We finally introduce the syntax/semantic of fuzzy temporal relations and describe the calculus of the fuzzy temporal algebra. Keywords—fuzzy temporal database;algebra; fuzzy temporal data model; fuzzy value; fuzzy degree
{"title":"Semantics of Fuzzy Temporal Databases Calculus","authors":"Liguo Deng, Zong Min Ma, Gang Zhang","doi":"10.1109/BMEI.2009.5305790","DOIUrl":"https://doi.org/10.1109/BMEI.2009.5305790","url":null,"abstract":"This paper present a fuzzy temporal data model for uncertainty/implicit events more precisely, and introduce fuzzy temporal complex event value relations, which combine the concept of fuzzy temporal relations with the idea of fuzzy temporal values in a uniform framework. We elaborate a model theoretic definition of fuzzy temporal information combination strategies, which has a rigorous foundation on fuzzy temporal theory. And then define an algebra for querying database instances. We prove that our data model and algebra for fuzzy temporal values generalizes the classical relational data model and algebra. Moreover, we show that under certain assumptions, all our algebraic operations are tractable. We finally introduce the syntax/semantic of fuzzy temporal relations and describe the calculus of the fuzzy temporal algebra. Keywords—fuzzy temporal database;algebra; fuzzy temporal data model; fuzzy value; fuzzy degree","PeriodicalId":6389,"journal":{"name":"2009 2nd International Conference on Biomedical Engineering and Informatics","volume":"27 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2009-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80214515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-10-30DOI: 10.1109/BMEI.2009.5305223
W. Yoo, J. Seo, D. Cho, K. Jang, J. Heo, J. Jun, Bongsoo Lee
We have measured temperature distribution using the infrared optical fibers during radiofrequency ablation (RFA). Infrared radiations generated from the water around inserted single-shaft plain electrode are transferred by the three silver halide optical fibers and are measured by a thermopile sensor array. Also, the output voltages of the thermopile sensor array are compared with those of the thermocouple recorder. It is expected that a non-contact temperature sensor using the infrared optical fibers can be developed for the real time temperature monitoring during RFA treatments based on the results of this study.
{"title":"Infrared Fiber-Optic Temperature Array Sensor for Radiofrequency Ablation","authors":"W. Yoo, J. Seo, D. Cho, K. Jang, J. Heo, J. Jun, Bongsoo Lee","doi":"10.1109/BMEI.2009.5305223","DOIUrl":"https://doi.org/10.1109/BMEI.2009.5305223","url":null,"abstract":"We have measured temperature distribution using the infrared optical fibers during radiofrequency ablation (RFA). Infrared radiations generated from the water around inserted single-shaft plain electrode are transferred by the three silver halide optical fibers and are measured by a thermopile sensor array. Also, the output voltages of the thermopile sensor array are compared with those of the thermocouple recorder. It is expected that a non-contact temperature sensor using the infrared optical fibers can be developed for the real time temperature monitoring during RFA treatments based on the results of this study.","PeriodicalId":6389,"journal":{"name":"2009 2nd International Conference on Biomedical Engineering and Informatics","volume":"40 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2009-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80700122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-10-30DOI: 10.1109/BMEI.2009.5305591
Liya Liang, Zesheng Zhang, Wei Sun, Yu-bin Wang
Inonotus Obliquus is a medicinal mushroom that is widely used in folk medicine in Russia, North Europe and China. The objective of this study was to evaluate the antioxidant and hypolipidemic capacities of Inonotus Obliquus polysaccharides(IOP). In vivo, the hyperlipidemic rats, induced by high-fat diet, were provided 100, 200, 500 mg/kg day of IOP for 8 weeks to evaluate their expected hypolipidemic activity. The results showed that Compared with MG, The administration of IOP did not show any effect on the body weight of the experimental rats, but significantly decreased the levels of LDL-C, TC, TG in serum, increased the activities of SOD and GSH-Px(P<0.05, P<0.01) and decreased the contents of MDA in serum, livers and hearts. These findings were further supported that IOP showed a noticeable inhibition against lipid oxidation induced by free radicals caused by high-fat diet intake on the basis of their antioxidant activities.
{"title":"Effect of the Inonotus Obliquus Polysaccharides on Blood Lipid Metabolism and Oxidative Stress of Rats Fed High-Fat Diet In Vivo","authors":"Liya Liang, Zesheng Zhang, Wei Sun, Yu-bin Wang","doi":"10.1109/BMEI.2009.5305591","DOIUrl":"https://doi.org/10.1109/BMEI.2009.5305591","url":null,"abstract":"Inonotus Obliquus is a medicinal mushroom that is widely used in folk medicine in Russia, North Europe and China. The objective of this study was to evaluate the antioxidant and hypolipidemic capacities of Inonotus Obliquus polysaccharides(IOP). In vivo, the hyperlipidemic rats, induced by high-fat diet, were provided 100, 200, 500 mg/kg day of IOP for 8 weeks to evaluate their expected hypolipidemic activity. The results showed that Compared with MG, The administration of IOP did not show any effect on the body weight of the experimental rats, but significantly decreased the levels of LDL-C, TC, TG in serum, increased the activities of SOD and GSH-Px(P<0.05, P<0.01) and decreased the contents of MDA in serum, livers and hearts. These findings were further supported that IOP showed a noticeable inhibition against lipid oxidation induced by free radicals caused by high-fat diet intake on the basis of their antioxidant activities.","PeriodicalId":6389,"journal":{"name":"2009 2nd International Conference on Biomedical Engineering and Informatics","volume":"29 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2009-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83080955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-10-30DOI: 10.1109/BMEI.2009.5305627
Qiuxiang Yang, Lihong Zhang
The algorithm and realization technique of network topology discovery is a very important technical indicator for a network management system’s quality. Based on the introduction of the network topology discovery theory and the research on MIB and the SNMP programming for Windows, this paper proposes the ideas and the methods of developing the common network topology discovery software. This technology has brought a good effect in the real applications.
{"title":"A Research on the Automatic Discovery Technology of Network Topology","authors":"Qiuxiang Yang, Lihong Zhang","doi":"10.1109/BMEI.2009.5305627","DOIUrl":"https://doi.org/10.1109/BMEI.2009.5305627","url":null,"abstract":"The algorithm and realization technique of network topology discovery is a very important technical indicator for a network management system’s quality. Based on the introduction of the network topology discovery theory and the research on MIB and the SNMP programming for Windows, this paper proposes the ideas and the methods of developing the common network topology discovery software. This technology has brought a good effect in the real applications.","PeriodicalId":6389,"journal":{"name":"2009 2nd International Conference on Biomedical Engineering and Informatics","volume":"192 1","pages":"1-3"},"PeriodicalIF":0.0,"publicationDate":"2009-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77760401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-10-30DOI: 10.1109/BMEI.2009.5305761
Ji-Hwan Kim, N. Thang, H. Suh, T. Rasheed, Tae-Seong Kim
In this paper, we have attempted forearm movement tracking in 3-D by estimating joint angles of a forearm with an inertial measurement unit (IMU) consisting of a triaxis accelerometer and gyroscope sensors. As the feedback of the forearm motion tracking, we have implemented a 3-D digital forearm model based on the kinematic chain theory utilizing super-quadric surfaces and joints, and attempted to control the digital arm with the estimated joint angles via Kalman filtering. Some preliminary experimental results are presented.
{"title":"Forearm Motion Tracking with Estimating Joint Angles from Inertial Sensor Signals","authors":"Ji-Hwan Kim, N. Thang, H. Suh, T. Rasheed, Tae-Seong Kim","doi":"10.1109/BMEI.2009.5305761","DOIUrl":"https://doi.org/10.1109/BMEI.2009.5305761","url":null,"abstract":"In this paper, we have attempted forearm movement tracking in 3-D by estimating joint angles of a forearm with an inertial measurement unit (IMU) consisting of a triaxis accelerometer and gyroscope sensors. As the feedback of the forearm motion tracking, we have implemented a 3-D digital forearm model based on the kinematic chain theory utilizing super-quadric surfaces and joints, and attempted to control the digital arm with the estimated joint angles via Kalman filtering. Some preliminary experimental results are presented.","PeriodicalId":6389,"journal":{"name":"2009 2nd International Conference on Biomedical Engineering and Informatics","volume":"36 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2009-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81321924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-10-30DOI: 10.1109/BMEI.2009.5305157
Biyan Wu
A mass sensor based on thin film bulk acoustic resonator (FBAR) is submitted in this paper. This sensor is fabricated by standard MEMS process as back-etch structure and its resonator curve is measured by Agilent Vector network analyer. The results show that this sensor's Q factor is above 610 and resonance frequency is about 1608MHz. This FBAR sensor is applied as humidity sensor. The reproducibility of this sensor’ S11and Q factor are evaluated.
{"title":"Mass Sensor Based on Thin Film Bulk Acoustic Resonator","authors":"Biyan Wu","doi":"10.1109/BMEI.2009.5305157","DOIUrl":"https://doi.org/10.1109/BMEI.2009.5305157","url":null,"abstract":"A mass sensor based on thin film bulk acoustic resonator (FBAR) is submitted in this paper. This sensor is fabricated by standard MEMS process as back-etch structure and its resonator curve is measured by Agilent Vector network analyer. The results show that this sensor's Q factor is above 610 and resonance frequency is about 1608MHz. This FBAR sensor is applied as humidity sensor. The reproducibility of this sensor’ S11and Q factor are evaluated.","PeriodicalId":6389,"journal":{"name":"2009 2nd International Conference on Biomedical Engineering and Informatics","volume":"18 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2009-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81386503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-10-30DOI: 10.1109/BMEI.2009.5302924
Xuezhong Zhou, Baoyan Liu
Traditional Chinese medicine (TCM) is a clinical medical discipline with clinical data as one of the main knowledge sources. The clinical information (e.g. symptoms, diagnoses and herb prescriptions) that is captured, generated and used by TCM physicians has complicated inter or intra relationships between the different elements. Due to the co- occurrence and combinational properties, TCM clinical data could naturally be represented by networks. This paper introduces a complex network analysis system to model and analyze the TCM clinical data. The system could automatically generate the networks from the clinical database, query the generated network data from the database and has the social network analysis abilities (e.g. measurements and community identification). It integrated the TCM knowledge (e.g. herb properties) to visualize the clinical data (e.g. herb prescriptions, symptoms and diagnoses) by networks, and can help acquire the core medical structures or relationships from the large-scale clinical data. It shows that the system provides a helpful platform for TCM clinical data analysis and the network analyses could generate clinically meaningful knowledge.
{"title":"Network Analysis System for Traditional Chinese Medicine Clinical Data","authors":"Xuezhong Zhou, Baoyan Liu","doi":"10.1109/BMEI.2009.5302924","DOIUrl":"https://doi.org/10.1109/BMEI.2009.5302924","url":null,"abstract":"Traditional Chinese medicine (TCM) is a clinical medical discipline with clinical data as one of the main knowledge sources. The clinical information (e.g. symptoms, diagnoses and herb prescriptions) that is captured, generated and used by TCM physicians has complicated inter or intra relationships between the different elements. Due to the co- occurrence and combinational properties, TCM clinical data could naturally be represented by networks. This paper introduces a complex network analysis system to model and analyze the TCM clinical data. The system could automatically generate the networks from the clinical database, query the generated network data from the database and has the social network analysis abilities (e.g. measurements and community identification). It integrated the TCM knowledge (e.g. herb properties) to visualize the clinical data (e.g. herb prescriptions, symptoms and diagnoses) by networks, and can help acquire the core medical structures or relationships from the large-scale clinical data. It shows that the system provides a helpful platform for TCM clinical data analysis and the network analyses could generate clinically meaningful knowledge.","PeriodicalId":6389,"journal":{"name":"2009 2nd International Conference on Biomedical Engineering and Informatics","volume":"27 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2009-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81594682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-10-30DOI: 10.1109/BMEI.2009.5302720
Lan Tao, Geng Liu, Xiaoli Wang, Lei Zhang
At present available computational methods can not predict selenoproteins correctly because of the special features of selenoproteins. It is known that there are some conservative sections around U in selenoproteins from previous research. So we bring forward a new method to predict selenoproteins based on motif recognition, we use Multiple Em for Motif Elicitation (MEME) to discover motif around U in selenoproteins and then predict selenoproteins based on the motif. The new method found all the selenoproteins in 9 seleno families expect one false positive in family of GPX1 and one in SelS. From the experiment, it is showed that this method can effectively predict almost all the selenoproteins in the known seleno families, and better than the methods of locating the position of U and blasting the Sec/Cys pairing based on handcraft.
由于硒蛋白的特殊性质，现有的计算方法不能正确预测硒蛋白。根据以往的研究，硒蛋白中U附近存在一些保守区。为此，我们提出了一种基于基序识别的预测硒蛋白的新方法，即利用多模态模序激发(Multiple Em for motif Elicitation, MEME)技术发现硒蛋白中U周围的基序，并基于该基序预测硒蛋白。新方法在9个硒蛋白家族中发现了除GPX1家族和SelS家族中的一个假阳性外的所有硒蛋白。实验结果表明，该方法能有效预测已知硒蛋白家族中几乎所有的硒蛋白，且优于手工定位U和爆破Sec/Cys配对的方法。
{"title":"Prediction of Selenoproteins Based on Motif Recognition","authors":"Lan Tao, Geng Liu, Xiaoli Wang, Lei Zhang","doi":"10.1109/BMEI.2009.5302720","DOIUrl":"https://doi.org/10.1109/BMEI.2009.5302720","url":null,"abstract":"At present available computational methods can not predict selenoproteins correctly because of the special features of selenoproteins. It is known that there are some conservative sections around U in selenoproteins from previous research. So we bring forward a new method to predict selenoproteins based on motif recognition, we use Multiple Em for Motif Elicitation (MEME) to discover motif around U in selenoproteins and then predict selenoproteins based on the motif. The new method found all the selenoproteins in 9 seleno families expect one false positive in family of GPX1 and one in SelS. From the experiment, it is showed that this method can effectively predict almost all the selenoproteins in the known seleno families, and better than the methods of locating the position of U and blasting the Sec/Cys pairing based on handcraft.","PeriodicalId":6389,"journal":{"name":"2009 2nd International Conference on Biomedical Engineering and Informatics","volume":"12 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2009-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82562905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-10-30DOI: 10.1109/BMEI.2009.5305205
Yue Feng, Yue Feng, J. Bai, X. Xia, Wenhua Zhao, Jiejie Dai, Xiaomei Sun, Qihan Li
—A real-time PCR assay based on the TaqMan chemistry was developed for reliable and quantitative detection of CD81 in Tupaia belangeri. The assay is performed with the ABI 7300 system using TaqMan probe and primers amplifying 118bp CD81 fragment of Tupaia belangeri. The standard curve for quantitation of target gene showed linearity over an at least 5-log DNA concentration range, represents10 3 to10 7 copies per reaction, with a correlation coefficient of 0.9996 (the slopes value-3.39 VS-3.32). Moreover, this protocol enabled detection of as little as 10 copies of CD81 cDNA in Tupaia belangeri liver tissues. The overall % coefficient of variation (%CV) for this assay was lower 5% with statistical significance due to in intra-assay 1.08% and inter-assay 0.16%, which indicated its high reproducibility. The new assay greatly improves current detection methods for CD81 evaluation, and this is the first report on the standardization and evaluation of a Tupaia belangeri CD81 RNA quantitation assay from China. As TaqMan real-time PCR enable the rapid and easy processing of a large number of samples, and can be used as a tool for monitoring progression of CD81 expression.
-建立了基于TaqMan化学的实时荧光定量PCR方法，可靠、定量地检测了图帕亚belangeri中CD81的含量。采用ABI 7300系统，使用TaqMan探针和引物扩增图皮亚belangeri的118bp CD81片段。目的基因定量标准曲线在至少5对数DNA浓度范围内呈线性关系，即每次反应为103 ~ 10.7个拷贝，相关系数为0.9996(斜率为3.39 vs . 3.32)。此外，该方案能够在图帕亚贝兰格里肝组织中检测到10个CD81 cDNA拷贝。结果表明，该方法的总变异系数(%CV)比原方法低5%，组内变异系数为1.08%，组间变异系数为0.16%，具有统计学意义，重复性高。新方法大大改进了现有的CD81评价检测方法，这是中国首个关于图帕亚贝兰杰CD81 RNA定量分析的标准化和评价报告。由于TaqMan实时PCR能够快速简便地处理大量样品，可以作为监测CD81表达进展的工具。
{"title":"Development of Real-Time PCR Assays for the Quantitative Detection of CD81 Receptor Gene of Hepatitis C Virus in Tupaia Belangeri","authors":"Yue Feng, Yue Feng, J. Bai, X. Xia, Wenhua Zhao, Jiejie Dai, Xiaomei Sun, Qihan Li","doi":"10.1109/BMEI.2009.5305205","DOIUrl":"https://doi.org/10.1109/BMEI.2009.5305205","url":null,"abstract":"—A real-time PCR assay based on the TaqMan chemistry was developed for reliable and quantitative detection of CD81 in Tupaia belangeri. The assay is performed with the ABI 7300 system using TaqMan probe and primers amplifying 118bp CD81 fragment of Tupaia belangeri. The standard curve for quantitation of target gene showed linearity over an at least 5-log DNA concentration range, represents10 3 to10 7 copies per reaction, with a correlation coefficient of 0.9996 (the slopes value-3.39 VS-3.32). Moreover, this protocol enabled detection of as little as 10 copies of CD81 cDNA in Tupaia belangeri liver tissues. The overall % coefficient of variation (%CV) for this assay was lower 5% with statistical significance due to in intra-assay 1.08% and inter-assay 0.16%, which indicated its high reproducibility. The new assay greatly improves current detection methods for CD81 evaluation, and this is the first report on the standardization and evaluation of a Tupaia belangeri CD81 RNA quantitation assay from China. As TaqMan real-time PCR enable the rapid and easy processing of a large number of samples, and can be used as a tool for monitoring progression of CD81 expression.","PeriodicalId":6389,"journal":{"name":"2009 2nd International Conference on Biomedical Engineering and Informatics","volume":"37 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2009-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82739281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
SHANLIN YU b, , MINGNA CHEN , QINGLI YANG, HONGSHENG ZHANG a Shandong Peanut Research Institute, Qingdao 266100, China b State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China Abstract Stearoyl-ACP desaturase (SAD), which catalyzes the first desaturation reaction of the most common pathway of unsaturated fatty acid synthesis in plants, plays a key role in determining the ratio of saturated to unsaturated fatty acids. Using Rapid Amplification of cDNA Ends (RACE) and a peanut cDNA library we had previously constructed, we isolated a 1499 bp cDNA of the SAD gene containing a 1218 bp complete open reading fragment (ORF). Sequence analysis revealed that peanut SAD had a high level of nucleotide and amino acid sequence homology to SAD from other plants. Characteristics of the deduced protein were predicted and analyzed using bioinformatic methods. Phylogenetic analysis showed that three different types of delta-9 fatty acid desaturases form separate clades, indicating that the three isozymes may have evolved independently. Expression analysis via real-time PCR indicated that expression levels of the SAD gene were markedly distinct in different peanut tissues and varieties. The results of the expression analysis in this study, combined with existing research, suggest that SAD may be involved in the regulation of plant seed growth and development.
b南京农业大学作物遗传与种质增效国家重点实验室，南京210095摘要硬脂酰acp去饱和酶(SAD)是催化植物中最常见的不饱和脂肪酸合成途径的第一次去饱和反应，在决定饱和脂肪酸与不饱和脂肪酸的比例中起着关键作用。利用fast Amplification of cDNA Ends (RACE)和我们之前构建的花生cDNA文库，我们分离出了一个1499 bp的SAD基因cDNA，其中包含一个1218 bp的完整开放阅读片段(ORF)。序列分析表明，花生SAD与其他植物的SAD具有较高的核苷酸和氨基酸同源性。利用生物信息学方法预测并分析了所得蛋白的特征。系统发育分析表明，三种不同类型的δ -9脂肪酸去饱和酶形成了不同的分支，表明这三种同工酶可能是独立进化的。实时荧光定量PCR分析表明，SAD基因在不同花生组织和品种中的表达水平存在显著差异。本研究的表达分析结果，结合已有研究，提示SAD可能参与植物种子生长发育的调控。
{"title":"Cloning, Characterization and Expression Analysis of a Stearoyl-ACP Desaturase Gene from Arachis Hypogaea","authors":"Shanlin Yu, Mingna Chen, Qingli Yang, Hongsheng Zhang","doi":"10.1109/BMEI.2009.5305511","DOIUrl":"https://doi.org/10.1109/BMEI.2009.5305511","url":null,"abstract":"SHANLIN YU b, , MINGNA CHEN , QINGLI YANG, HONGSHENG ZHANG a Shandong Peanut Research Institute, Qingdao 266100, China b State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China Abstract Stearoyl-ACP desaturase (SAD), which catalyzes the first desaturation reaction of the most common pathway of unsaturated fatty acid synthesis in plants, plays a key role in determining the ratio of saturated to unsaturated fatty acids. Using Rapid Amplification of cDNA Ends (RACE) and a peanut cDNA library we had previously constructed, we isolated a 1499 bp cDNA of the SAD gene containing a 1218 bp complete open reading fragment (ORF). Sequence analysis revealed that peanut SAD had a high level of nucleotide and amino acid sequence homology to SAD from other plants. Characteristics of the deduced protein were predicted and analyzed using bioinformatic methods. Phylogenetic analysis showed that three different types of delta-9 fatty acid desaturases form separate clades, indicating that the three isozymes may have evolved independently. Expression analysis via real-time PCR indicated that expression levels of the SAD gene were markedly distinct in different peanut tissues and varieties. The results of the expression analysis in this study, combined with existing research, suggest that SAD may be involved in the regulation of plant seed growth and development.","PeriodicalId":6389,"journal":{"name":"2009 2nd International Conference on Biomedical Engineering and Informatics","volume":"9 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2009-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89620114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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