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Influence of citrate buffer and flash heating in enhancing the sensitivity of ratiometric genosensing of Hepatitis C virus using plasmonic gold nanoparticles 柠檬酸缓冲液和闪蒸加热对提高等离子体金纳米颗粒比例基因感测丙型肝炎病毒敏感性的影响
IF 4.7 Q2 NANOSCIENCE & NANOTECHNOLOGY Pub Date : 2021-10-26 DOI: 10.1186/s40486-021-00134-3
Hrishikesh Shashi Prakash, Pranay Amruth Maroju, Naga Sai Sriteja Boppudi, Aniket Balapure, Ramakrishnan Ganesan, Jayati Ray Dutta

Gold nanoparticles (Au NPs) based technology has been shown to possess enormous potential in the viral nucleic acid diagnosis. Despite significant advancement in this domain, the existing literature reveals the diversity in the conditions employed for hybridization and tagging of thiolated nucleic acid probes over the Au NPs. Here we employ the probe sequence derived from the Hepatitis C virus to identify the optimal hybridization and thiol-Au NP tagging conditions. In a typical polymerase chain reaction, the probes are initially subjected to flash heating at elevated temperatures to obtain efficient annealing. Motivated by this, in the current study, the hybridization between the target and the antisense oligonucleotide (ASO) has been studied at 65 °C with and without employing flash heating at temperatures from 75 to 95 °C. Besides, the efficiency of the thiolated ASO’s tagging over the Au NPs with and without citrate buffer has been explored. The study has revealed the beneficial role of flash heating at 95 °C for efficient hybridization and the presence of citrate buffer for rapid and effective thiol tagging over the Au NPs. The combinatorial effect of these conditions has been found to be advantageous in enhancing the sensitivity of ratiometric genosensing using Au NPs.

基于金纳米颗粒(Au NPs)的技术在病毒核酸诊断中具有巨大的潜力。尽管在这一领域取得了重大进展,但现有文献揭示了硫代核酸探针在Au NPs上杂交和标记的条件的多样性。在这里,我们利用来自丙型肝炎病毒的探针序列来确定最佳的杂交和硫醇-金NP标记条件。在典型的聚合酶链反应中,探针首先在高温下进行闪速加热以获得有效的退火。基于此,在本研究中,我们在65°C条件下研究了靶标与反义寡核苷酸(ASO)的杂交,并在75 ~ 95°C的温度下进行了闪速加热和不闪速加热。此外,还探讨了巯基化ASO在有和没有柠檬酸盐缓冲液的Au NPs上的标记效率。该研究揭示了95°C闪速加热对高效杂交的有益作用,以及柠檬酸缓冲液的存在对Au NPs上快速有效的硫醇标记的有益作用。这些条件的组合效应已被发现有利于提高使用Au NPs的比率基因传感的灵敏度。
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引用次数: 0
Continuous preparation of bicelles using hydrodynamic focusing method for bicelle to vesicle transition 用水动力聚焦法连续制备小囊体,使小囊体向囊泡过渡
IF 4.7 Q2 NANOSCIENCE & NANOTECHNOLOGY Pub Date : 2021-10-25 DOI: 10.1186/s40486-021-00133-4
SungHak Choi, BongSu Kang, Toshinori Shimanouchi, Keesung Kim, HoSup Jung

Bicelle is one of the most stable phospholipid assemblies, which has tremendous applications in the research areas for drug delivery or structural studies of membrane proteins owing to its bio-membrane mimicking characteristics and high thermal stability. However, the conventional preparation method for bicelle demands complicated manufacturing processes and a long time so that the continuous synthesis method of bicelle using microfluidic chip has been playing an important role to expand its feasibility. We verified the general availability of hydrodynamic focusing method with microfluidic chip for bicelle synthesis using various kinds of lipids which have a phase transition temperature ranged from − 2 to 41 °C. Bicelle can be formed only when the inside temperature of microfluidic chip was over the phase transition temperature. Moreover, the concentration condition for bicelle formation varied depending on the lipids. Furthermore, the transition process characteristics from bicelle to vesicle were analyzed by effective q-value, mixing time and dilution condition. We verified that the size of transition vesicles was controlled according to the effective q-value, mixing time, and temperature.

Bicelle是最稳定的磷脂组合之一,由于其具有生物膜模拟特性和较高的热稳定性,在药物传递和膜蛋白结构研究领域有着巨大的应用。然而,传统的单晶硅制备方法制造工艺复杂,耗时长,因此利用微流控芯片连续合成单晶硅的方法在扩大其可行性方面发挥着重要作用。我们用微流控芯片验证了流体动力聚焦法合成双胞体的普遍有效性,该方法使用的各种脂质的相变温度范围为- 2至41°C。只有当微流控芯片内部温度超过相变温度时,才能形成双胞体。此外,脂质不同,单细胞形成的浓度条件也不同。并从有效q值、混合时间、稀释条件等方面分析了从单胞到囊泡的转化过程特征。我们验证了根据有效q值、混合时间和温度来控制过渡囊泡的大小。
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引用次数: 0
Copper micromesh-based lightweight transparent conductor with short response time for wearable heaters 用于可穿戴式加热器的基于铜微网的轻质透明导体,响应时间短
IF 4.7 Q2 NANOSCIENCE & NANOTECHNOLOGY Pub Date : 2021-10-25 DOI: 10.1186/s40486-021-00132-5
Han-Jung Kim, Yoonkap Kim

Thickness-controlled transparent conducting films (TCFs) were fabricated by transfer printing a 100 nm thick Cu micromesh structure onto poly(vinyl alcohol) (PVA) substrates of different thicknesses (~ 50, ~ 80, and ~ 120 μm) to develop a lightweight transparent wearable heater with short response time. The Cu mesh-based TCF fabricated on a ~ 50 µm thick PVA substrate exhibited excellent optical and electrical properties with a light transmittance of 86.7% at 550 nm, sheet resistance of ~ 10.8 Ω/sq, and figure-of-merit of approximately 236, which are comparable to commercial indium tin oxide film-based transparent conductors. The remarkable flexibility of the Cu mesh-based TCF was demonstrated through cyclic mechanical bending tests. In addition, the Cu mesh-based TCF with ~ 50 μm thick PVA substrate demonstrated a fast Joule heating performance with a thermal response time of ~ 18.0 s and a ramping rate of ~ 3.0 ℃/s under a driving voltage of 2.5 V. Lastly, the reliable response and recovery characteristics of the Cu mesh/PVA film-based transparent heater were confirmed through the cyclic power test. We believe that the results of this study is useful in the development of flexible transparent heaters, including lightweight deicing/defogging films, wearable sensors/actuators, and medical thermotherapy pads.

通过在不同厚度(~ 50、~ 80和~ 120 μm)的聚乙烯醇(PVA)衬底上转移打印厚度为100 nm的Cu微网结构,制备了厚度可控的透明导电薄膜(tcf),开发了响应时间短的轻质透明可穿戴加热器。在~ 50µm厚的PVA衬底上制备的Cu网基TCF具有优异的光学和电学性能,在550 nm处透光率为86.7%,片电阻为~ 10.8 Ω/sq,品质因数约为236,与商业氧化铟锡薄膜透明导体相当。通过循环力学弯曲试验证明了铜网格基TCF具有显著的柔韧性。此外,在2.5 V驱动电压下,采用~ 50 μm厚PVA衬底的Cu网格TCF具有快速焦耳加热性能,热响应时间为~ 18.0 s,升温速率为~ 3.0℃/s。最后,通过循环功率试验,验证了Cu网/PVA薄膜透明加热器的可靠响应和恢复特性。我们相信这项研究的结果对柔性透明加热器的开发是有用的,包括轻质除冰/除雾薄膜、可穿戴传感器/致动器和医用热疗垫。
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引用次数: 0
Multiple ratiometric nanothermometry using semiconductor BiFeO3 nanowires and quantitative validation of thermal sensitivity 使用半导体BiFeO3纳米线的多比率纳米测温法和热敏性的定量验证
IF 3.6 Q2 NANOSCIENCE & NANOTECHNOLOGY Pub Date : 2021-10-14 DOI: 10.36227/techrxiv.16780459
K. Prashanthi, K. K. Krishna Mohan, Ž. Antić, K. Ahadi, M. Dramićanin
Here, we report a very sensitive, non-contact, ratio-metric, and robust luminescence-based temperature sensing using a combination of conventional photoluminescence (PL) and negative thermal quenching (NTQ) mechanisms of semiconductor BiFeO 3 (BFO) nanowires. Using this approach, we have demonstrated the absolute thermal sensitivity of ~ 10 mK −1 over the 300–438 K temperature range and the relative sensitivity of 0.75% K −1 at 300 K. Further, we have validated thermal sensitivity of BFO nanowires quantitatively using linear regression and analytical hierarchy process (AHP) and found close match with the experimental results. These results indicated that BFO nanowires are excellent candidates for developing high‐performance luminescence-based temperature sensors. Graphical abstract
在这里,我们报道了一种非常灵敏、非接触、比率度量和稳健的基于发光的温度传感,该温度传感使用半导体BiFeO3(BFO)纳米线的传统光致发光(PL)和负热猝灭(NTQ)机制的组合。使用这种方法,我们已经证明了 ~ 在300–438 K温度范围内为10 mK−1,在300 K下的相对灵敏度为0.75%K−1。此外,我们还使用线性回归和层次分析法(AHP)定量验证了BFO纳米线的热敏性,并发现与实验结果非常吻合。这些结果表明,BFO纳米线是开发基于高性能发光的温度传感器的优秀候选者。图形摘要
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引用次数: 2
Green synthesized plant-based silver nanoparticles: therapeutic prospective for anticancer and antiviral activity 绿色合成植物基纳米银:抗癌和抗病毒活性的治疗前景
IF 3.6 Q2 NANOSCIENCE & NANOTECHNOLOGY Pub Date : 2021-05-03 DOI: 10.1186/s40486-021-00131-6
Nancy Jain, Priyanshu Jain, Devyani Rajput, Umesh Kumar Patil

Nanotechnology holds an emerging domain of medical science as it can be utilized virtually in all areas. Phyto-constituents are valuable and encouraging candidates for synthesizing green silver nanoparticles (AgNPs) which possess great potentials toward chronic diseases. This review gives an overview of the Green approach of AgNPs synthesis and its characterization. The present review further explores the potentials of Phyto-based AgNPs toward anticancer and antiviral activity including its probable mechanism of action. Green synthesized AgNPs prepared by numerous medicinal plants extract are critically reviewed for cancer and viral infection. Thus, this article mainly highlights green synthesized Phyto-based AgNPs with their potential applications for cancer and viral infection including mechanism of action and therapeutic future prospective in a single window.

纳米技术是医学科学的一个新兴领域,因为它几乎可以应用于所有领域。植物成分是合成绿色银纳米粒子(AgNPs)的有价值和令人鼓舞的候选物质,对慢性疾病具有巨大的潜力。本文综述了AgNPs的绿色合成方法及其表征。本文将进一步探讨植物基AgNPs在抗癌和抗病毒方面的潜力及其可能的作用机制。多种药用植物提取物制备的绿色合成AgNPs在癌症和病毒感染方面受到了广泛的关注。因此,本文主要从单窗口的角度介绍绿色合成植物AgNPs及其在癌症和病毒感染中的潜在应用,包括作用机制和治疗前景。
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引用次数: 62
High speed silicon wet anisotropic etching for applications in bulk micromachining: a review 高速硅湿各向异性刻蚀在体微加工中的应用综述
IF 3.6 Q2 NANOSCIENCE & NANOTECHNOLOGY Pub Date : 2021-02-22 DOI: 10.1186/s40486-021-00129-0
Prem Pal, Veerla Swarnalatha, Avvaru Venkata Narasimha Rao, Ashok Kumar Pandey, Hiroshi Tanaka, Kazuo Sato

Wet anisotropic etching is extensively employed in silicon bulk micromachining to fabricate microstructures for various applications in the field of microelectromechanical systems (MEMS). In addition, it is most widely used for surface texturing to minimize the reflectance of light to improve the efficiency of crystalline silicon solar cells. In wet bulk micromachining, the etch rate is a major factor that affects the throughput. Slower etch rate increases the fabrication time and therefore is of great concern in MEMS industry where wet anisotropic etching is employed to perform the silicon bulk micromachining, especially to fabricate deep cavities and freestanding microstructures by removal of underneath material through undercutting process. Several methods have been proposed to increase the etch rate of silicon in wet anisotropic etchants either by physical means (e.g. agitation, microwave irradiation) or chemically by incorporation of additives. The ultrasonic agitation during etching and microwave irradiation on the etchants increase the etch rate. However, ultrasonic method may rupture the fragile structures and microwave irradiation causes irradiation damage to the structures. Another method is to increase the etching temperature towards the boiling point of the etchant. The etching characteristics of pure potassium hydroxide solution (KOH) is studied near the boiling point of KOH, while surfactant added tetramethylammonium hydroxide (TMAH) is investigated at higher temperature to increase the etch rate. Both these studies have shown a potential way of increasing the etch rate by elevating the temperature of the etchants to its boiling point, which is a function of concentration of etch solution. The effect of various kinds of additives on the etch rate of silicon is investigated in TMAH and KOH. In this paper, the additives which improve the etch rate have been discussed. Recently the effect of hydroxylamine (NH2OH) on the etching characteristics of TMAH and KOH is investigated in detail. The concentration of NH2OH in TMAH/KOH is varied to optimize the etchant composition to obtain improved etching characteristics especially the etch rate and undercutting which are important parameters for increasing throughput. In this article, different methods explored to improve the etch rate of silicon have been discussed so that the researchers/scientists/engineers can get the details of these methods in a single reference.

湿法各向异性蚀刻技术广泛应用于硅体微加工中,用于制造微机电系统(MEMS)领域的各种微结构。此外,它最广泛地用于表面纹理,以尽量减少光的反射率,以提高晶体硅太阳能电池的效率。在湿体微加工中,蚀刻速率是影响吞吐量的主要因素。较慢的蚀刻速率增加了制造时间,因此在MEMS行业中是一个非常值得关注的问题,在MEMS行业中,湿各向异性蚀刻被用来执行硅体微加工,特别是通过下切工艺去除底部材料来制造深腔和独立的微结构。提出了几种提高硅在湿各向异性蚀刻剂中蚀刻速率的方法,有的是物理方法(如搅拌、微波辐照),有的是化学方法(加入添加剂)。在蚀刻过程中采用超声波搅拌和微波辐照可以提高蚀刻速率。然而,超声方法可能使脆弱的结构破裂,微波辐射对结构造成辐射损伤。另一种方法是将蚀刻温度提高到蚀刻剂的沸点。研究了纯氢氧化钾溶液(KOH)在沸点附近的蚀刻特性,并在较高温度下研究了表面活性剂四甲基氢氧化铵(TMAH)的加入,以提高蚀刻速率。这两项研究都表明,通过提高蚀刻剂的温度到沸点来提高蚀刻速率是一种潜在的方法,沸点是蚀刻溶液浓度的函数。研究了不同添加剂对硅在TMAH和KOH中蚀刻速率的影响。本文讨论了提高腐蚀速率的添加剂。近年来研究了羟胺(NH2OH)对TMAH和KOH刻蚀特性的影响。通过改变TMAH/KOH中NH2OH的浓度来优化蚀刻剂的组成,从而提高蚀刻性能,特别是蚀刻速率和下切量,这是提高产量的重要参数。在这篇文章中,探讨了不同的方法来提高硅的蚀刻率,以便研究人员/科学家/工程师可以在一个单一的参考文献中得到这些方法的细节。
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引用次数: 25
One-step electropolymerization of methylene blue films on highly flexible carbon fiber electrode as supercapacitors 在高柔性碳纤维电极上一步电聚合亚甲基蓝薄膜作为超级电容器
IF 3.6 Q2 NANOSCIENCE & NANOTECHNOLOGY Pub Date : 2021-02-22 DOI: 10.1186/s40486-021-00130-7
Leandro Marques Samyn, Rajendran Suresh Babu, Mani Devendiran, Ana Lucia Ferreira de Barros

Energy crisis and environmental pollution have been one of the major global issues. In this regard, the search for new energy storage materials is cheap, flexible and high-performance supercapacitors electrode which has become intensive. Also, reducing the amount of organic dyes polluting in water is a great significance. Herein, one-step electropolymerization of methylene blue on carbon fiber and the resulting films were applied to the supercapacitor. The high performance is associated to the outstanding conductivity, electrochemical stability and superior mechanical flexibility of carbon fiber. A new flexible electrode for supercapacitors was successfully fabricated by demonstrating with a good electrochemical performance and a promising alternative to reduce the water pollution.

能源危机和环境污染已成为全球性的重大问题之一。在这方面,寻找廉价、柔性和高性能的超级电容器电极的新型储能材料已变得密集。同时,减少水中的有机染料污染也具有重要意义。本文将亚甲基蓝在碳纤维上一步电聚合,并将聚合膜应用于超级电容器。碳纤维具有优异的导电性、电化学稳定性和优异的机械柔韧性。成功制备了一种新型超级电容器柔性电极,具有良好的电化学性能,是减少水污染的理想选择。
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引用次数: 14
Study on thickness-dependence characteristics of bismuth ferrite (BFO) for ultraviolet (UV) photodetector application 用于紫外(UV)光电探测器的铋铁氧体(BFO)厚度依赖特性的研究
IF 3.6 Q2 NANOSCIENCE & NANOTECHNOLOGY Pub Date : 2021-01-04 DOI: 10.1186/s40486-020-00128-7
Shahnaz Kossar, R. Amiruddin, Asif Rasool

The present research work reports on the fabrication of ultraviolet (UV) photodetectors using bismuth ferrite (BiFeO3, BFO) thin films with varying thickness. Using the spray pyrolysis technique, BFO thin films were deposited on the glass substrate at 673?K. The deposited BFO thin films were characterized by Raman and FTIR spectroscopic analysis. The morphological analysis reveals uniform grain distribution for the prepared BFO samples. The optical analysis reveals that transmittance value decreases upon an increase in the thickness of BFO thin films and the calculated optical band gap value lies between 2.0 to 2.3?eV. The varying thickness of the BFO active layer was stacked between ITO and Al electrodes and the current–voltage (I–V) characteristics of the fabricated ITO/BFO/Al devices were studied under dark and UV illumination (λ?=?365?nm). It was observed that BFO with an optimum thickness (365?nm) exhibits higher photoresponsivity of 110?mA/W with an external quantum efficiency (EQE) of 37.30%. The impact of different thickness of the BFO active layer, the role of adsorption and desorption of oxygen (O2) molecules upon the surface of BFO layers towards UV photoresponse characteristics were investigated.

本文报道了利用不同厚度的铋铁氧体(BiFeO3, BFO)薄膜制备紫外光电探测器的研究工作。采用喷雾热解技术,在673 K温度下在玻璃基板上沉积BFO薄膜。用拉曼光谱和傅里叶红外光谱对制备的BFO薄膜进行了表征。形态学分析表明制备的BFO样品晶粒分布均匀。光学分析表明,透射率随BFO薄膜厚度的增加而减小,计算得到的光学带隙值在2.0 ~ 2.3 eV之间。在ITO和Al电极之间堆叠不同厚度的BFO有源层,并在暗光源和紫外光源(λ = 365nm)下研究了制备的ITO/BFO/Al器件的电流-电压(I-V)特性。结果表明,最佳厚度为365nm的BFO具有较高的光响应率,达到110?mA/W,外量子效率(EQE)为37.30%。研究了BFO活性层厚度、氧分子在BFO活性层表面的吸附和解吸对紫外光响应特性的影响。
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引用次数: 27
All-soft multiaxial force sensor based on liquid metal for electronic skin 电子皮肤用液态金属全软多轴力传感器
IF 3.6 Q2 NANOSCIENCE & NANOTECHNOLOGY Pub Date : 2021-01-04 DOI: 10.1186/s40486-020-00126-9
Kyuyoung Kim, Junseong Ahn, Yongrok Jeong, Jungrak Choi, Osman Gul, Inkyu Park

Electronic skin (E-skin) capable of detecting various physical stimuli is required for monitoring external environments accurately. Here, we report an all-soft multiaxial force sensor based on liquid metal microchannel array for electronic skin applications. The proposed sensor is composed of stretchable elastomer and Galinstan, a eutectic gallium-indium alloy, providing a high mechanical flexibility and electro-mechanical durability. Liquid metal microchannel arrays are fabricated in multilayer and positioned along a dome structure to detect multi-directional forces, supported by numerical simulation results. By adjusting the height of the dome, we could control the response of the multiaxial sensor with respect to the deflection. As a demonstration of multiaxial force sensing, we were able to monitor the direction of multidirectional forces using a finger by the response of liquid metal microchannel arrays. This research could be applied to various fields including soft robotics, wearable devices, and smart prosthetics for artificial intelligent skin applications.

电子皮肤(E-skin)需要能够检测各种物理刺激,以准确监测外部环境。在这里,我们报告了一种基于液态金属微通道阵列的全软多轴力传感器,用于电子皮肤。该传感器由可拉伸弹性体和Galinstan(一种共晶镓铟合金)组成,具有很高的机械灵活性和机电耐用性。采用多层结构制备液态金属微通道阵列,沿圆顶结构定位,用于检测多向力,并得到数值模拟结果的支持。通过调整穹顶的高度,我们可以控制多轴传感器对挠度的响应。作为多轴力传感的演示,我们能够通过液态金属微通道阵列的响应,用手指监测多向力的方向。这项研究可以应用于软机器人、可穿戴设备、人工智能皮肤应用的智能假肢等多个领域。
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引用次数: 14
Hollow polydimethylsiloxane (PDMS) foam with a 3D interconnected network for highly sensitive capacitive pressure sensors 中空聚二甲基硅氧烷(PDMS)泡沫,具有3D互连网络,用于高灵敏度电容压力传感器
IF 3.6 Q2 NANOSCIENCE & NANOTECHNOLOGY Pub Date : 2020-12-14 DOI: 10.1186/s40486-020-00127-8
Dong Hwan Kim, Young Jung, Kyungkuk Jung, Dong Hwa Kwak, Dong Min Park, Myung Gyu Shin, Hyeong Jun Tak, Jong Soo Ko

We propose a highly sensitive capacitive pressure sensor made of hollow polydimethylsiloxane (PDMS) foam with a three-dimensional network structure. The stiffness of the foam is adjusted by the viscosity of the PDMS solution. The fabricated PDMS-30 (PDMS 30 wt%) foam shows extremely high porosity (>?86%) approximately 19 times that of bare PDMS (PDMS 100 wt%) foam. Capacitive pressure sensors fabricated using the foam possess high sensitivity, good compressibility (up to 80% strain), and consistent output characteristics in a 2000-cycle test.

我们提出了一种具有三维网络结构的中空聚二甲基硅氧烷(PDMS)泡沫材料制成的高灵敏度电容式压力传感器。泡沫的刚度由PDMS溶液的粘度来调节。制备的PDMS-30 (PDMS 30 wt%)泡沫具有极高的孔隙率(>?86%),约为裸PDMS (PDMS 100 wt%)泡沫的19倍。使用泡沫制成的电容式压力传感器具有高灵敏度,良好的压缩性(高达80%的应变),并且在2000次循环测试中具有一致的输出特性。
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引用次数: 13
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Micro and Nano Systems Letters
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