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Reversible, stable and uniform SERS in a Y-shaped microfluidic chip: chemical imaging of concentration gradients Y 形微流体芯片中可逆、稳定和均匀的 SERS:浓度梯度的化学成像
IF 2.3 4区 工程技术 Q2 INSTRUMENTS & INSTRUMENTATION Pub Date : 2024-07-03 DOI: 10.1007/s10404-024-02740-0
Fabien Chauvet

Imaging of chemical composition in microfluidic chips is addressed using Surface Enhanced Raman Spectroscopy (SERS). The Y-shaped SERS microfluidic chip used is fabricated by xurography and an electrodeposition method is employed to form a thin nanostructured silver layer over the bottom glass wall of the main microchannel. Used as an immobilized SERS substrate, this layer of silver nanocrystals exhibits an analytical enhancement factor of 5.10(^4) uniformly distributed over its surface (RSD < 7%). These good performances allow the quantitative imaging of transverse diffusion profiles of Crystal Violet (CV) at low concentrations ((10^{-8}-10^{-6}) mol/L). The SERS measurement turns out to be reversible at high laser power and this is explained by the thermal desorption of adsorbed CV (photothermal effect). However, too high heating leads to a low amount of adsorbed species and a low SERS signal. This effect is limited by using a fast enough flow inducing a cooling effect. A compromise must be found between laser power and liquid flow rate to enable a reversible and sensitive SERS measurement in the chip. These findings should contribute to the development of imaging, in microfluidic conditions, of the spatiotemporal dynamics of weakly concentrated key molecules involved in chemical, biochemical or biological processes.

利用表面增强拉曼光谱(SERS)技术对微流控芯片中的化学成分进行成像。所使用的 Y 型 SERS 微流控芯片是通过 Xurography 技术制造的,并采用电沉积方法在主微通道的底部玻璃壁上形成了一层薄薄的纳米结构银层。作为固定的 SERS 基底,这层纳米银晶体在其表面均匀分布,显示出 5.10(^4) 的分析增强因子(RSD <7%)。这些良好的性能允许在低浓度((10^{-8}-10^{-6}) mol/L)下对水晶紫(CV)的横向扩散曲线进行定量成像。在高激光功率下,SERS 测量结果是可逆的,这可以用吸附的 CV 的热解吸(光热效应)来解释。然而,过高的加热会导致吸附物种的数量减少和 SERS 信号降低。通过使用足够快的流速来产生冷却效应,可以限制这种效应。必须在激光功率和液体流速之间找到一个折衷方案,才能在芯片中进行可逆和灵敏的 SERS 测量。这些发现将有助于在微流控条件下对化学、生化或生物过程中涉及的弱浓缩关键分子的时空动态进行成像。
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引用次数: 0
A low-cost method of fabricating Parylene-SU-8 micro-nanofluidic chip by thermal nanoimprint and multilayer compositing 利用热纳米压印和多层复合技术制造聚对二甲苯-SU-8 微纳流体芯片的低成本方法
IF 2.3 4区 工程技术 Q2 INSTRUMENTS & INSTRUMENTATION Pub Date : 2024-06-28 DOI: 10.1007/s10404-024-02743-x
Lei Sun, Zhifu Yin, Ran Guo

A new fabrication of micro-nano fluidic chips by thermal nanoimprint and multilayer compositing is proposed. The nano-channels with different aspect ratios were first manufactured by thermal nanoimprinting and Parylene layer coating, and the effect of parylene layer thickness on aspect ratio is studied. Then SU-8 layers with the micro channels and liquid reservoirs were superposed by ultraviolet exposure and secondary bonding to form a multilayer micro-nano fluidic chip. By optimizing the bonding parameters, a Parylene-SU-8 micro-nano fluidic chip with 200 μm wide and 8 μm deep micro-channels, cross-linked with 78 nm wide and 288 nm deep nano-channels was constructed, of which the maximum bonding strength is 0.92 MPa. This method has the advantages of simple process and low cost, which is suitable for mass manufacturing of micro-nano fluidic chips.

本文提出了一种利用热纳米压印和多层复合技术制造微纳流体芯片的新方法。首先通过热纳米压印和聚对二甲苯层涂层制造出不同长径比的纳米通道,并研究了聚对二甲苯层厚度对长径比的影响。然后,通过紫外曝光和二次键合将带有微通道和储液器的 SU-8 层叠加在一起,形成多层微纳流体芯片。通过优化键合参数,构建了宽 200 μm、深 8 μm、交联有 78 nm 宽、288 nm 深纳米通道的对二甲苯-SU-8 微纳流体芯片,其最大键合强度为 0.92 MPa。该方法具有工艺简单、成本低廉等优点,适用于微纳流体芯片的大规模制造。
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引用次数: 0
Lung-on-a-chip composed of styrene-butadiene-styrene nano-fiber/porous PDMS composite membranes with cyclic triaxial stimulation 由苯乙烯-丁二烯-苯乙烯纳米纤维/多孔 PDMS 复合膜组成的肺芯片受到循环三轴刺激
IF 2.3 4区 工程技术 Q2 INSTRUMENTS & INSTRUMENTATION Pub Date : 2024-06-24 DOI: 10.1007/s10404-024-02739-7
Yuru You, Changling Zhang, Zhixiang Guo, Feng Xu, Daoheng Sun, Junjie Xia, Songyue Chen

The physiological function of lung is strongly correlated with its unique structural microenvironment and mechanical stimulation. Most existing lung-on-a-chips (LOCs) do not replicate the key physiological structure and stimulation of human lung, reducing their reliability in application. In this study, a scaffold structure of a styrene-butadiene-styrene (SBS) nanofiber and porous honeycomb polydime-thylsiloxane (PDMS) composite membrane was developed to construct an alveolar air-blood barrier that mimics the alveolar characteristics of flexibility, cross-scale structure, and triaxial mechanical stimulation. By combining micro-fluidic and electrospinning technology, a biomimetic LOC with dynamic triaxial cyclic strain was realized. The composite membrane had a Young’s modulus of 0.54 ± 0.05 MPa and was capable of 8–12% strain at 1 kPa air pressure. We monocultured and co-cultured human non-small cell lung cancer cells stably expressing red fluorescent protein (A549-RFP) with human umbilical vein endothelial cell stably expressing green fluorescent protein (HUVECs-GFP) within the chip. A multi-layered structure of epithelial cell layer-basal layer-endothelial cell layer, similar to the air-blood barrier in vivo, was constructed. The LOC was proved to be an initial foundation for creating in vitro alveolar physiological models, and could be a potential platform for application in physiology, pathology, toxicology, drug screening, and customized medicine.

肺的生理功能与其独特的微环境结构和机械刺激密切相关。现有的肺芯片(LOCs)大多无法复制人体肺部的关键生理结构和刺激,降低了其应用的可靠性。本研究开发了一种由苯乙烯-丁二烯-苯乙烯(SBS)纳米纤维和多孔蜂窝状聚二甲基硅氧烷(PDMS)复合膜组成的支架结构,用于构建肺泡气血屏障,以模拟肺泡的柔韧性、跨尺度结构和三轴机械刺激特性。通过结合微流体和电纺丝技术,实现了具有动态三轴循环应变的仿生物 LOC。复合膜的杨氏模量为 0.54 ± 0.05 兆帕,在 1 千帕气压下可承受 8-12% 的应变。我们将稳定表达红色荧光蛋白(A549-RFP)的人非小细胞肺癌细胞与稳定表达绿色荧光蛋白(HUVECs-GFP)的人脐静脉内皮细胞分别单培养和共培养在芯片中。这样就构建了一个类似于体内气血屏障的上皮细胞层-基底层-内皮细胞层的多层结构。事实证明,LOC 是创建体外肺泡生理模型的初步基础,可作为生理学、病理学、毒理学、药物筛选和定制医学的潜在应用平台。
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引用次数: 0
Advances in modeling permeability and selectivity of the blood-brain barrier using microfluidics 利用微流体技术建立血脑屏障渗透性和选择性模型的进展
IF 2.3 4区 工程技术 Q2 INSTRUMENTS & INSTRUMENTATION Pub Date : 2024-06-23 DOI: 10.1007/s10404-024-02741-z
Jindi Sun, Shang Song

The blood-brain barrier (BBB) protects the brain by actively allowing the entry of ions and nutrients while limiting the passage of from toxins and pathogens. A healthy BBB has low permeability and high selectivity to maintain normal brain functions. Increased BBB permeability can result from neurological diseases and traumatic injuries. Modern engineering technologies such as microfluidics and fabrication techniques have advanced the development of BBB models to simulate the basic functions of BBB. However, the intrinsic BBB properties are difficult to replicate. Existing in vitro BBB models demonstrate inconsistent BBB permeability and selectivity due to variations in microfluidic design, cell types and arrangement, expression of tight junction (TJ) proteins, and use of shear stress. Specifically, microfluidic designs have flow channels of different sizes, complexity, topology, and modular structure. Different cell types are selected to mimic various physiological conditions. These factors make it challenging to compare results obtained using different experimental setups. This paper highlights key factors that play important roles in influencing microfluidic models and discusses how these factors contribute to permeability and selectivity of the BBB models.

血脑屏障(BBB)通过积极允许离子和营养物质的进入,同时限制毒素和病原体的通过来保护大脑。健康的血脑屏障具有低渗透性和高选择性,以维持正常的大脑功能。神经系统疾病和外伤会导致 BBB 渗透性增加。微流控和制造技术等现代工程技术推动了 BBB 模型的发展,以模拟 BBB 的基本功能。然而,BBB 的内在特性很难复制。由于微流控设计、细胞类型和排列、紧密连接(TJ)蛋白的表达以及剪切应力的使用存在差异,现有的体外 BBB 模型显示出不一致的 BBB 通透性和选择性。具体来说,微流体设计有不同大小、复杂程度、拓扑结构和模块结构的流道。选择不同的细胞类型是为了模拟各种生理条件。这些因素使得比较使用不同实验装置获得的结果具有挑战性。本文强调了影响微流控模型的关键因素,并讨论了这些因素如何影响 BBB 模型的渗透性和选择性。
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引用次数: 0
Finger-operated pumping platform for microfluidic preparation of nanoparticles 用于微流控制备纳米粒子的指控泵平台
IF 2.3 4区 工程技术 Q2 INSTRUMENTS & INSTRUMENTATION Pub Date : 2024-06-14 DOI: 10.1007/s10404-024-02738-8
Ahmed Azmeer, Ibraheem Kanan, Ghaleb A. Husseini, Mohamed Abdelgawad

Microfluidic preparation of nanoparticles (NPs) offers many advantages over traditional bench-top preparation techniques, including better control over particle size and higher uniformity. Although many studies have reported the use of low-cost microfluidic chips for nanoparticle synthesis, the technology is still expensive due to the high cost of the pumps needed to generate the required flows inside microchannels. Here, we present a low-cost finger-operated constant-pressure pumping platform capable of generating pressures as high as 120 kPa using finger-operated pumping caps that can be attached to any pop bottle. The platform costs around $208 and enables the generation of flow rate ratios (FRR) of up to 47:1 for the continuous flow synthesis of NPs. The pump has a resolution of 500 Pa per stroke and exhibits stable pressures for up to a few hours. To show the functionality of the proposed pump, we used it to prepare pegylated liposomes and poly lactic-co-glycolic acid (PLGA) nanoparticles with sizes ranging from 47 nm to 250 nm with an average polydispersity of 20% using commercially available micromixer chips and in-house made hydrodynamic flow focusing devices. We believe this platform will render microfluidic preparation of NPs accessible to any laboratory with minimal capabilities.

与传统的台式制备技术相比,微流体制备纳米粒子(NPs)具有许多优势,包括更好地控制粒度和更高的均匀性。尽管许多研究都报道了使用低成本微流控芯片合成纳米粒子的方法,但由于在微通道内产生所需流量所需的泵成本高昂,因此该技术的成本仍然很高。在这里,我们展示了一种低成本的手指操作恒压泵平台,利用可连接到任何汽水瓶上的手指操作泵盖,能够产生高达 120 kPa 的压力。该平台的成本约为 208 美元,能够产生高达 47:1 的流速比 (FRR),用于 NPs 的连续流合成。该泵每个冲程的分辨率为 500 Pa,压力稳定时间长达数小时。为了展示所建议的泵的功能,我们使用它制备了聚乙二醇化脂质体和聚乳酸-聚乙二醇酸(PLGA)纳米粒子,这些粒子的尺寸从 47 纳米到 250 纳米不等,平均多分散性为 20%,使用的是市售的微混合器芯片和内部制造的流体动力流聚焦装置。我们相信,这一平台将使任何实验室都能以最低限度的能力进行 NPs 的微流控制备。
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引用次数: 0
Engineering free-standing electrospun PLLCL fibers on microfluidic platform for cell alignment 在微流体平台上设计用于细胞排列的独立电纺 PLLCL 纤维
IF 2.3 4区 工程技术 Q2 INSTRUMENTS & INSTRUMENTATION Pub Date : 2024-06-07 DOI: 10.1007/s10404-024-02736-w
Özüm Yildirim-Semerci, Ahu Arslan-Yildiz

Here, a PLLCL-on-chip platform was developed by direct electrospinning of poly (L-lactide-co-ε-caprolactone) (PLLCL) on polymethyl methacrylate (PMMA) microfluidic chips. Designed microchip provides the electrospinning of free-standing aligned PLLCL fibers which eliminates limitations of conventional electrospinning. Besides, aligned fiber structure favors cell alignment through contactless manipulation. Average fiber diameter, and fiber alignment was evaluated by SEM analyses, then, leakage profile of microchip was investigated. 3D cell culture studies were conducted using HeLa and NIH-3T3 cells, and nearly 85% cell viability was observed in PLLCL-on-chip for 15 days, while cell viability of 2D control started to decrease after 7 days based on Live dead and Alamar Blue analyses. These findings emphasize biocompatibility of PLLCL-on-chip platform for 3D cell culture and its ability to mimic extracellular matrix (ECM). Immunostaining results prove that PLLCL-on-chip platform favors the secretion of ECM proteins compared to control groups, and cytoskeletons of cells were in aligned orientation in PLLCL-on-chip, while they were in random orientation in control groups. Overall, these results demonstrate that the developed platform is suitable for the formation of various 3D cell culture models and a potential candidate for cell alignment studies.

Graphical Abstract

本文通过在聚甲基丙烯酸甲酯(PMMA)微流体芯片上直接电纺聚(L-内酯-共ε-己内酯)(PLLCL),开发了一种 PLLCL 芯片平台。设计的微芯片可以电纺独立排列的 PLLCL 纤维,从而消除了传统电纺的局限性。此外,排列整齐的纤维结构有利于通过非接触式操作使细胞排列整齐。通过扫描电子显微镜分析评估了纤维的平均直径和纤维排列,然后研究了微芯片的泄漏曲线。使用 HeLa 和 NIH-3T3 细胞进行了三维细胞培养研究,结果表明,在 PLLCL 芯片上培养 15 天后,细胞存活率接近 85%,而根据活死细胞和阿拉玛蓝分析,二维对照组的细胞存活率在 7 天后开始下降。这些发现强调了用于三维细胞培养的片上 PLLCL 平台的生物相容性及其模拟细胞外基质(ECM)的能力。免疫染色结果证明,与对照组相比,芯片上 PLLCL 平台有利于 ECM 蛋白的分泌,而且芯片上 PLLCL 平台的细胞骨架排列整齐,而对照组的细胞骨架排列无序。总之,这些结果表明所开发的平台适用于形成各种三维细胞培养模型,是细胞排列研究的潜在候选者。
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引用次数: 0
Simulation analysis and experimental verification of thermodynamic characteristics of integrated droplet digital PCR chip 集成液滴数字 PCR 芯片热力学特性的仿真分析与实验验证
IF 2.3 4区 工程技术 Q2 INSTRUMENTS & INSTRUMENTATION Pub Date : 2024-06-04 DOI: 10.1007/s10404-024-02737-9
Xiangkai Meng, Luyang Duanmu, Ping Gong

In order to reduce the influence of the thermal conductivity of the digital polymerase chain reaction (dPCR) chip material and the temperature distribution of the droplet collection chamber on the amplification effect, an optimized integrated dPCR chip was designed. The heat conduction of the designed dPCR gene chip was simulated by COMSOL finite element model, which provided theoretical basis for the design and fabrication of the chip. Three-dimensional ht models of dPCR microarray under steady state and transient conditions were established. The thermodynamic simulation of dPCR gene chip was carried out by changing the material, thickness, structure and width of droplet collection chamber. During the high temperature denaturation stage of amplification, the temperature characteristics were analyzed, and the surface temperature, heating curve, isotherm, thermal expansion and other results of the dPCR gene chip were obtained, and the structural parameters of the chip design were optimized to provide guidance for the subsequent chip design. The results showed that the internal temperature uniformity of the COC sample was higher than other materials. The chip has a thickness of 2 mm and the collection chamber has a width of 4 mm, which was better suited to meet the requirements of PCR reaction. The PCR amplification device was established, and the uniformity of temperature distribution of the fabricatedchip was verified by thermal imager. The results showed that the heat conduction speed was fast, the heat conduction was uniform, and the uniformity was less than ± 0.5 °C. Therefore, under the premise of meeting the quantity of microdroplet generation, the chip designed in this paper has excellent heat conduction performance.

为了减少数字聚合酶链式反应(dPCR)芯片材料的导热性和液滴收集腔的温度分布对扩增效果的影响,设计了一种优化的集成 dPCR 芯片。利用 COMSOL 有限元模型模拟了所设计的 dPCR 基因芯片的热传导过程,为芯片的设计和制造提供了理论依据。建立了稳态和瞬态条件下 dPCR 芯片的三维 ht 模型。通过改变液滴收集腔的材料、厚度、结构和宽度,对 dPCR 基因芯片进行了热力学模拟。分析了扩增高温变性阶段的温度特性,得到了 dPCR 基因芯片的表面温度、加热曲线、等温线、热膨胀等结果,优化了芯片设计的结构参数,为后续芯片设计提供了指导。结果表明,COC 样品的内部温度均匀性高于其他材料。芯片厚度为 2 毫米,收集腔宽度为 4 毫米,能较好地满足 PCR 反应的要求。建立了 PCR 扩增装置,并用热成像仪验证了所制芯片温度分布的均匀性。结果表明,热传导速度快,热传导均匀,均匀度小于±0.5 °C。因此,在满足微滴产生量的前提下,本文设计的芯片具有优异的热传导性能。
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引用次数: 0
Biological structural study for the blood casson fluid flow in catheterized diverging tapered stenosed arteries with emerging shaped nanoparticles: application in drug delivery 导管分流锥形狭窄动脉中血液卡松流体流动的生物结构研究:在药物输送中的应用
IF 2.3 4区 工程技术 Q2 INSTRUMENTS & INSTRUMENTATION Pub Date : 2024-05-21 DOI: 10.1007/s10404-024-02735-x
Noreen Sher Akbar, Maimona Rafiq, Taseer Muhammad, Metib Alghamdi

The current research focuses on investigating the influence of magnetic forces and differently shaped nanoparticles within diverging tapering arteries afflicted with stenoses, utilizing a blood flow model. A notable aspect of this study is the exploration of metallic nanoparticles of various shapes within a water-based fluid medium, a research area that remains largely unexplored. To simulate blood flow dynamics, a radially symmetric yet axially non-symmetric stenosis configuration is employed, providing insights into the complex flow patterns within diseased arteries. A significant contribution of our research lies in the analysis of the symmetrical distribution of wall shearing stresses and their correlation with resistive impedance. Moreover, we investigate the progressive rise of these quantities in tandem with stenosis severity. Through numerical simulations, we evaluate several flow parameters, including velocity, temperature, resistance impedance, boundary shear stress, and shearing stress at the stenosis throat. These assessments provide a comprehensive understanding of the multifaceted effects of nanoparticle shape and magnetic forces on blood flow characteristics within tapered arteries. Furthermore, our study explores the graphical representation of various flow quantities across a spectrum of relevant parameters for Cu-blood systems. By examining different types of tapered arteries, particularly diverging tapering configurations, we gain insights into the intricate interplay between arterial geometry, fluid rheology, and nanoparticle behavior.

目前的研究重点是利用一个血流模型,研究磁力和不同形状的纳米粒子对患有狭窄的分叉锥形动脉的影响。本研究的一个显著特点是在水基流体介质中探索各种形状的金属纳米粒子,而这一研究领域在很大程度上仍未被开发。为了模拟血流动力学,我们采用了径向对称但轴向非对称的狭窄配置,从而深入了解了病变动脉内复杂的流动模式。我们研究的一个重要贡献在于分析了管壁剪应力的对称分布及其与阻抗的相关性。此外,我们还研究了这些量随狭窄严重程度而逐渐增加的情况。通过数值模拟,我们评估了多个流动参数,包括速度、温度、阻抗、边界剪应力和狭窄喉部的剪应力。通过这些评估,我们可以全面了解纳米粒子形状和磁力对锥形动脉内血流特征的多方面影响。此外,我们的研究还探索了铜血液系统相关参数范围内各种流动量的图形表示方法。通过研究不同类型的锥形动脉,特别是发散锥形配置,我们深入了解了动脉几何形状、流体流变学和纳米粒子行为之间错综复杂的相互作用。
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引用次数: 0
Effect of finite spatial and temporal resolutions on super-resolution particle tracking velocimetry for pressure-driven flow in a nanochannel 有限空间分辨率和时间分辨率对纳米通道中压力驱动流动的超分辨率粒子跟踪测速仪的影响
IF 2.3 4区 工程技术 Q2 INSTRUMENTS & INSTRUMENTATION Pub Date : 2024-05-14 DOI: 10.1007/s10404-024-02733-z
Minori Tanaka, Yo Saeki, Itsuo Hanasaki, Yutaka Kazoe

With developments of nanofluidics, understanding the behavior of fluids confined in nanospaces becomes important. Particle tracking is an efficient approach, but in nanospaces, it often suffers from the finite temporal resolution, which causes the Brownian displacement of nanoparticles, and the finite spatial resolution due to the decreased signal-to-noise ratio of nanoparticle images, both of which are factors that can cause artifacts. Therefore, in the present study, we simulated nanoparticle tracking velocimetry based on the particle dynamics given by the Langevin equation to evaluate the artifacts. The results revealed that for measurement of the velocity distribution of pressure-driven flow in a 400 nm nanochannel utilizing 60 nm tracer nanoparticles, high-speed (temporal resolution: Δt ≤ 360 µs) and super-resolution (spatial resolution: Δz ≤ 25 nm) measurement is required for errors less than 10%, while insufficient resolution causes an artifact that results in a flattened velocity distribution compared with the original flow profile. The proposed resolutions were experimentally verified by defocusing nanoparticle tracking velocimetry developed by our group. As the simulation predicted, at longer temporal resolution and larger spatial resolution, the measured nanoparticle velocity distribution in the nanochannel indicated a parabolic flow profile but became flattened because of the artifacts. In contrast, at measurement resolutions within the proposed range, the velocity distribution close to the profile given by the Hagen-Poiseuille equation, which was considered to be the actual flow profile, was successfully obtained. This work provides a guideline for nanoscale flow measurements and will accelerate the understanding of specific transport phenomena in nanospaces.

随着纳米流体技术的发展,了解纳米空间中的流体行为变得非常重要。粒子跟踪是一种高效的方法,但在纳米空间中,它往往受到有限时间分辨率和有限空间分辨率的影响,前者会导致纳米粒子的布朗位移,后者则会降低纳米粒子图像的信噪比,这两个因素都会造成伪影。因此,在本研究中,我们基于朗格文方程给出的粒子动力学模拟了纳米粒子跟踪测速,以评估伪影。结果表明,在利用 60 纳米示踪纳米粒子的 400 纳米通道中测量压力驱动流动的速度分布时,需要高速(时间分辨率:Δt ≤ 360 µs)和超分辨率(空间分辨率:Δz ≤ 25 纳米)测量才能使误差小于 10%,而分辨率不足则会造成伪影,使速度分布与原始流动曲线相比变得扁平。我们小组开发的散焦纳米粒子跟踪测速仪在实验中验证了所提出的分辨率。正如模拟预测的那样,在较长的时间分辨率和较大的空间分辨率下,纳米通道中测得的纳米粒子速度分布显示出抛物线流动曲线,但由于伪影而变得扁平。与此相反,在建议范围内的测量分辨率下,成功获得了接近哈根-普瓦耶方程给出的速度分布曲线,这被认为是实际的流动曲线。这项工作为纳米级流动测量提供了指导,并将加速对纳米空间中特定传输现象的理解。
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引用次数: 0
Design and proof-of-concept of a micropillar-based microfluidic chip for trapping and culture of single cells 设计并验证基于微柱的微流体芯片,用于捕获和培养单细胞
IF 2.3 4区 工程技术 Q2 INSTRUMENTS & INSTRUMENTATION Pub Date : 2024-05-09 DOI: 10.1007/s10404-024-02734-y
Thu Hang Nguyen, Ngoc Anh Nguyen Thi, Hang Bui Thu, Tung Thanh Bui, Trinh Chu Duc, Loc Do Quang

Single-cell analysis provides a groundbreaking avenue for exploring cell-to-cell variation, the heterogeneity of cell responses to stimuli, and the impact of DNA sequence variations on cell phenotypes. A crucial facet of this analytical approach involves the refinement of techniques for effective single-cell trapping and sustained culture. This study introduces a microfluidic platform based on micropillars for hydrodynamic trapping and prolonged cultivation of individual cells. The proposed biochip design, termed three-micropillars based microfluidic (3µPF) structure, incorporates interleaved trap units, each featuring three-micropillars based microfluidic structure strategically designated to trap single cells, enhance the surface area of cells exposed to the culture medium, and enable dynamic culture, continuous waste removal. This configuration aims to mitigate adverse effects associated with bioparticle collisions compared to conventional trap units. The study employs finite element method to conduct a comprehensive numerical investigation into the operational mechanism of the microfluidic device. The simulation results show that the filled trap unit demonstrates a low-velocity magnitude, reducing shear stress on cells and facilitating extended culture. The hydrodynamic single-cell trap mechanism of the proposed device was also verified. The insights derived from this work are pivotal for optimizing the device and guiding future experimental examinations, thus contributing significantly to the progression of single-cell analysis techniques.

单细胞分析为探索细胞间变异、细胞对刺激反应的异质性以及 DNA 序列变异对细胞表型的影响提供了一条突破性途径。这种分析方法的一个重要方面是完善有效的单细胞捕获和持续培养技术。本研究介绍了一种基于微柱的微流体平台,用于单个细胞的流体动力捕获和长时间培养。所提出的生物芯片设计被称为基于三微柱的微流体(3µPF)结构,它包含交错的捕获单元,每个单元都有三个基于微柱的微流体结构,这些微流体结构被战略性地指定用于捕获单个细胞,增加细胞暴露于培养基的表面积,并实现动态培养和持续清除废物。与传统的捕集装置相比,这种配置旨在减轻生物颗粒碰撞带来的不利影响。研究采用有限元法对微流体装置的运行机制进行了全面的数值研究。模拟结果表明,填充式捕集装置具有低速幅度,可减少细胞受到的剪切应力,有利于延长培养时间。此外,还验证了拟议装置的流体力学单细胞捕获机制。这项工作得出的见解对于优化该装置和指导未来的实验研究至关重要,从而为单细胞分析技术的发展做出了重大贡献。
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引用次数: 0
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Microfluidics and Nanofluidics
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