In this work, the combined impacts of electroosmosis and peristaltic processes are investigated to better understand the behavior of fluid flow in a symmetric channel. The Poisson–Boltzmann equation is included into the Navier–Stokes equations to account for the electrokinetic effects in micropolar fluid model. The fluid motion caused by electric fields is effectively described by incorporating electrokinetic variables in these equations. Under the premise of a low Reynolds number and small amplitude, the linearized equations are resolved. Partial differential equations are solved to yield analytical formulations for the velocity and pressure fields. As opposed to earlier research, our analysis explores the combined impacts of electroosmosis and peristaltic motion in symmetric channels. By considering these mechanisms together, we gain a comprehensive understanding of fluid movement and manipulation in microchannels. According to research on modifying the properties of fluid flow, zeta potential, applied voltage, and channel shape all affect the velocity of electroosmotic flow. In addition, the flow rate is impacted by the peristaltic motion-induced periodic pressure changes. In addition, the combined effects of peristalsis and electroosmosis show promise for accurate and efficient regulation of fluid flow in microchannels. The study reveals that the micropolar parameter modifications (0–100) have little effect whereas adjusting the coupling parameter (0–1) modifies electroosmotic peristaltic flow. Center streamlines are trapped and then aligned in a length-dependent way by the interaction of electric fields. Several microfluidic applications, including mixing, pumping, and particle manipulation, are affected by the findings of this research. The electroosmosis and peristaltic processes may be understood and used to create sophisticated microfluidic devices and lab-on-a-chip systems. This development has the potential to greatly improve performance and functionality in industries like chemical analysis, biomedical engineering, and other areas needing precise fluid control at the microscale.