Climate change, coupled with various abiotic and biotic stresses, continues to cause substantial global losses in crop yields, threatening food security. Innovative technologies, such as nanotechnology, have shown promising potential to address these challenges by improving agricultural productivity and sustainability. Manganese (Mn), an essential micronutrient, plays a crucial role in photosynthesis, nitrogen assimilation, reactive oxygen species (ROS) scavenging, hormone signaling, pathogen defense, structural polymer synthesis, and interactions with plant-associated microbes. As a vital cofactor in the oxygen-evolving complex (OEC) of photosystem II (PSII), Mn catalyzes the water-splitting reaction essential for photosynthesis. Nanoscale Mn based nanoparticles (NPs), including Mn, MnO, Mn₂O₃, MnO₂, Mn3O4, MnFe₂O₄, Mn₀.₅Zn₀.₅Fe₂O₄, biochar-modified MnO₂ (BC@MnO₂), and composite nanomaterials like chitosan/silver/Mn₀.₅Zn₀.₅Fe₂O₄ (Cs/Ag/MnMgFe₂O₄), offer superior bioavailability, reactivity, and stress mitigation compared to bulk Mn sources or untreated controls. Studies report up to a 45% increase in growth parameters and a 49% increase in yield with Mn NP application compared to untreated plants under field conditions. Additionally, these NPs modulate signaling, regulate stress-related gene expression, and activate defense mechanisms, thereby supporting overall plant health and productivity. Optimizing Mn based NPs synthesis, functionalization, and application strategies will be crucial for ensuring safety and maximizing efficacy. Although Mn based NPs hold great potential for sustainable agriculture, their widespread adoption demands thorough research and validation to ensure agricultural benefits while maintaining ecological responsibility.
{"title":"Utilizing manganese-based nanoparticles for enhancing environmental stress resilience and productivity of plants","authors":"Pallavi Sharma, Ambuj Bhushan Jha, Rama Shanker Dubey","doi":"10.1039/d5en00292c","DOIUrl":"https://doi.org/10.1039/d5en00292c","url":null,"abstract":"Climate change, coupled with various abiotic and biotic stresses, continues to cause substantial global losses in crop yields, threatening food security. Innovative technologies, such as nanotechnology, have shown promising potential to address these challenges by improving agricultural productivity and sustainability. Manganese (Mn), an essential micronutrient, plays a crucial role in photosynthesis, nitrogen assimilation, reactive oxygen species (ROS) scavenging, hormone signaling, pathogen defense, structural polymer synthesis, and interactions with plant-associated microbes. As a vital cofactor in the oxygen-evolving complex (OEC) of photosystem II (PSII), Mn catalyzes the water-splitting reaction essential for photosynthesis. Nanoscale Mn based nanoparticles (NPs), including Mn, MnO, Mn₂O₃, MnO₂, Mn3O4, MnFe₂O₄, Mn₀.₅Zn₀.₅Fe₂O₄, biochar-modified MnO₂ (BC@MnO₂), and composite nanomaterials like chitosan/silver/Mn₀.₅Zn₀.₅Fe₂O₄ (Cs/Ag/MnMgFe₂O₄), offer superior bioavailability, reactivity, and stress mitigation compared to bulk Mn sources or untreated controls. Studies report up to a 45% increase in growth parameters and a 49% increase in yield with Mn NP application compared to untreated plants under field conditions. Additionally, these NPs modulate signaling, regulate stress-related gene expression, and activate defense mechanisms, thereby supporting overall plant health and productivity. Optimizing Mn based NPs synthesis, functionalization, and application strategies will be crucial for ensuring safety and maximizing efficacy. Although Mn based NPs hold great potential for sustainable agriculture, their widespread adoption demands thorough research and validation to ensure agricultural benefits while maintaining ecological responsibility.","PeriodicalId":73,"journal":{"name":"Environmental Science: Nano","volume":"34 1","pages":""},"PeriodicalIF":8.131,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143805967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yidi Sun, Tao Zong, Qi Wu, Xuetao Wang, Huijing Hou, Xiaoping Xin, Jigan Xie, Yuhao Zhou, Jianchang Yang
The use of nitrogen fertilizers leads to substantial nitrogen losses and subsequent environmental pollution. Biochar (BC) demonstrates considerable potential for enhancing N fixation and reducing emissions, but it frequently induces a liming effect that increases ammonia (NH3) volatilization. Nano-biochar (NBC) is attracting considerable attention due to its higher surface energy. However, little information is available whether it could enhance nitrogen adsorption and reduce NH3 volatilization. Therefore, this study utilized one-step ball milling method to produce NBC, characterized its physicochemical properties, investigated its effects and mechanisms on NH4+-N adsorption and NH3 volatilization. Our results showed that, specific surface area, pore volume and acidic functional groups of NBC were higher than those of bulk BC, while pore diameter, zeta potential and pH were the opposite. Which was more conducive to promoting adsorption, the maximum adsorption amount of NBC for NH4+-N was 6.880 mg/g, approximately 1.9 times that of bulk BC. The adsorption process conformed to the Langmuir adsorption isotherm model and the pseudo-second-order kinetic equation, indicating that the adsorption was monolayer and chemical. The primary adsorption mechanisms included physical adsorption, ion exchange, electrostatic and π-π interactions. The addition of 0.30%-30% of bulk BC and NBC reduced NH3 volatilization by 6.40%-31.50% and 5.00%-42.20%, relative to no BC addition, respectively. The main reason for lower NH3 volatilization observed with NBC was its ability to improve soil mineral nitrogen content, mineralization and nitrification rates, and decrease urease activity and pH. Therefore, NBC is a green and efficient adsorbent for reducing nitrogen emissions.
{"title":"Fabrication of rice straw nano-biochar by ball milling for efficient adsorption of ammonium nitrogen and reduction of ammonia volatilization: Effects and Mechanisms","authors":"Yidi Sun, Tao Zong, Qi Wu, Xuetao Wang, Huijing Hou, Xiaoping Xin, Jigan Xie, Yuhao Zhou, Jianchang Yang","doi":"10.1039/d5en00103j","DOIUrl":"https://doi.org/10.1039/d5en00103j","url":null,"abstract":"The use of nitrogen fertilizers leads to substantial nitrogen losses and subsequent environmental pollution. Biochar (BC) demonstrates considerable potential for enhancing N fixation and reducing emissions, but it frequently induces a liming effect that increases ammonia (NH3) volatilization. Nano-biochar (NBC) is attracting considerable attention due to its higher surface energy. However, little information is available whether it could enhance nitrogen adsorption and reduce NH3 volatilization. Therefore, this study utilized one-step ball milling method to produce NBC, characterized its physicochemical properties, investigated its effects and mechanisms on NH4+-N adsorption and NH3 volatilization. Our results showed that, specific surface area, pore volume and acidic functional groups of NBC were higher than those of bulk BC, while pore diameter, zeta potential and pH were the opposite. Which was more conducive to promoting adsorption, the maximum adsorption amount of NBC for NH4+-N was 6.880 mg/g, approximately 1.9 times that of bulk BC. The adsorption process conformed to the Langmuir adsorption isotherm model and the pseudo-second-order kinetic equation, indicating that the adsorption was monolayer and chemical. The primary adsorption mechanisms included physical adsorption, ion exchange, electrostatic and π-π interactions. The addition of 0.30%-30% of bulk BC and NBC reduced NH3 volatilization by 6.40%-31.50% and 5.00%-42.20%, relative to no BC addition, respectively. The main reason for lower NH3 volatilization observed with NBC was its ability to improve soil mineral nitrogen content, mineralization and nitrification rates, and decrease urease activity and pH. Therefore, NBC is a green and efficient adsorbent for reducing nitrogen emissions.","PeriodicalId":73,"journal":{"name":"Environmental Science: Nano","volume":"37 1","pages":""},"PeriodicalIF":8.131,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143797807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nickel is generally found in trace amounts in the environment and can be beneficial to living organisms, but it is also an environmental contaminant of high concern, primarily due to anthropogenic releases. Fe oxides play an significant role in the behavior and fate of Ni in the environment, as they can interact with metal cations. However, the interactions between magnetite (Fe3O4) and Ni are not well described, and in particular the effect of magnetite stoichiometry (Fe(II)/Fe(III) = R) is not well considered. Ni adsorption experiments were performed on stoichiometric (R0.5) and oxidized (R0.1) magnetite as a function of Ni concentration and pH under anaerobic conditions. Samples were analyzed by transmission electron microscopy, X-ray absorption spectroscopy (XAS) and magnetic circular dichroism at the Ni L2,3-edges and XAS at the Ni K-edge. At high Ni concentrations, Ni precipitates as Ni(OH)2 on the magnetite surface, but also as distinct sheet-like particles. At low Ni concentrations, high energy resolution fluorescence detection (HERFD) XAS analyses at the Ni K-edge revealed Ni incorporation into R0.5 magnetite and surface adsorption of Ni onto R0.1 magnetite. The present results were compared with those previously published for Co, which revealed unexpected distinct behavior of Ni and Co. This element-specific binding mechanisms highlights the unique properties of magnetite compared to other naturally occurring iron oxides (e.g. goethite, hematite), for which Ni and Co binding mechanisms are similar. Taken together, these results will help predicting the behavior and fate of Ni under environmental conditions in the presence of magnetite, but also to synthesize magnetite nanoparticles doped by the addition of Ni with interesting magnetic properties.
{"title":"Nickel binding with magnetite nanoparticles","authors":"Laura Fablet, Mathieu Pédrot, Fadi Choueikani, Isabelle Kieffer, Olivier Proux, anne-catherine pierson-wickmann, Vyria Cagnart, Takumi Yomogida, Rémi Marsac","doi":"10.1039/d4en01114g","DOIUrl":"https://doi.org/10.1039/d4en01114g","url":null,"abstract":"Nickel is generally found in trace amounts in the environment and can be beneficial to living organisms, but it is also an environmental contaminant of high concern, primarily due to anthropogenic releases. Fe oxides play an significant role in the behavior and fate of Ni in the environment, as they can interact with metal cations. However, the interactions between magnetite (Fe3O4) and Ni are not well described, and in particular the effect of magnetite stoichiometry (Fe(II)/Fe(III) = R) is not well considered. Ni adsorption experiments were performed on stoichiometric (R0.5) and oxidized (R0.1) magnetite as a function of Ni concentration and pH under anaerobic conditions. Samples were analyzed by transmission electron microscopy, X-ray absorption spectroscopy (XAS) and magnetic circular dichroism at the Ni L2,3-edges and XAS at the Ni K-edge. At high Ni concentrations, Ni precipitates as Ni(OH)2 on the magnetite surface, but also as distinct sheet-like particles. At low Ni concentrations, high energy resolution fluorescence detection (HERFD) XAS analyses at the Ni K-edge revealed Ni incorporation into R0.5 magnetite and surface adsorption of Ni onto R0.1 magnetite. The present results were compared with those previously published for Co, which revealed unexpected distinct behavior of Ni and Co. This element-specific binding mechanisms highlights the unique properties of magnetite compared to other naturally occurring iron oxides (e.g. goethite, hematite), for which Ni and Co binding mechanisms are similar. Taken together, these results will help predicting the behavior and fate of Ni under environmental conditions in the presence of magnetite, but also to synthesize magnetite nanoparticles doped by the addition of Ni with interesting magnetic properties.","PeriodicalId":73,"journal":{"name":"Environmental Science: Nano","volume":"6 1","pages":""},"PeriodicalIF":8.131,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143789994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In recent years, microbial pollution has become a serious environmental problem, and the release of microorganisms into the water environment seriously threatens human health. As environment-friendly and low-cost antibacterial agents, Mg(OH)2 nanoparticles (M-NPs) have garnered considerable attention for their small size, innocuity, no drug resistance, chemical stability and thermal stability. However, little is known about the physiological changes that bacteria undergo in the presence of M-NPs. In this work, the antibacterial mechanism of M-NPs synthesized by applying the coprecipitation method was investigated using Escherichia coli (E. coli) as a model system. The oxygen vacancies on the M-NP surface, which can produce reactive oxygen species (ROS, ·O2−, H2O2, and ·OH), were examined via O2-temperature programmed desorption (O2-TPD). Abnormality in three central metabolic pathways (energy, glucose and tricarboxylic acid cycle) induced by M-NPs was detected by analyzing the activity of respiratory chain dehydrogenase, gluconokinase (GK) and succinate dehydrogenase (SDH). The downregulated activity and gene expression levels of GK confirmed that M-NPs play an inhibitory role, and these physiological changes result in cell death. Thus, M-NPs have great potential in the field of preventing and controlling microbial pollution.
{"title":"Construction and characterization of environment-friendly antibacterial Mg(OH)2 nanoparticles and their induced metabolic changes in Escherichia coli","authors":"Ying Wang, Fuming Wang, Xuyang Feng, Haoyou Jiang, Hualin Zhang, Yongfang Qian, Botian Zhu, Yaping Huang, Yimin Zhu","doi":"10.1039/d4en01023j","DOIUrl":"https://doi.org/10.1039/d4en01023j","url":null,"abstract":"In recent years, microbial pollution has become a serious environmental problem, and the release of microorganisms into the water environment seriously threatens human health. As environment-friendly and low-cost antibacterial agents, Mg(OH)<small><sub>2</sub></small> nanoparticles (M-NPs) have garnered considerable attention for their small size, innocuity, no drug resistance, chemical stability and thermal stability. However, little is known about the physiological changes that bacteria undergo in the presence of M-NPs. In this work, the antibacterial mechanism of M-NPs synthesized by applying the coprecipitation method was investigated using <em>Escherichia coli</em> (<em>E. coli</em>) as a model system. The oxygen vacancies on the M-NP surface, which can produce reactive oxygen species (ROS, ·O<small><sub>2</sub></small><small><sup>−</sup></small>, H<small><sub>2</sub></small>O<small><sub>2</sub></small>, and ·OH), were examined <em>via</em> O<small><sub>2</sub></small>-temperature programmed desorption (O<small><sub>2</sub></small>-TPD). Abnormality in three central metabolic pathways (energy, glucose and tricarboxylic acid cycle) induced by M-NPs was detected by analyzing the activity of respiratory chain dehydrogenase, gluconokinase (GK) and succinate dehydrogenase (SDH). The downregulated activity and gene expression levels of GK confirmed that M-NPs play an inhibitory role, and these physiological changes result in cell death. Thus, M-NPs have great potential in the field of preventing and controlling microbial pollution.","PeriodicalId":73,"journal":{"name":"Environmental Science: Nano","volume":"183 1","pages":""},"PeriodicalIF":8.131,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143789819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Multi-walled carbon nanotubes (MW) and tetracycline (TC) usually co-occur in the environment and can threaten plant growth. However, little is known about their combined effect on plant growth. We conducted hydroponic experiments to investigate the uptake, transport, and compartmentalization of TC in Chrysanthemum coronarium L. in the presence of MW, and the combined effects of TC and MW on the root environment were also studied. The results revealed that the presence of MW reduced the concentration of TC in the four subcellular fractions, both in the leaves and roots, compared with TC alone. Co-pollution with TC and MW stimulated the production and accumulation of superoxide anions (O2.-), and hydrogen peroxide (H2O2) in the leaves and roots compared to TC alone, leading to an increase in the malondialdehyde content; this inhibited photosynthesis by reducing the activity of ribulose-1,5-bisphosphate carboxylase and dehydrogenase activity, resulting in a decrease in the dry weight of leaves and roots. The increased O2.- and H2O2 contents induced superoxide dismutase and catalase activities to alleviate oxidative damage. In addition, compared with single TC contamination, the co-application of TC and MW significantly increased the concentrations of oxalic acid and formic acid in root secretions, stimulated the activity of microorganisms, and improved autochthonous input and humification of dissolved organic matter in the growth medium. High-throughput sequencing revealed that Proteobacteria were the dominant bacteria in the medium solution across all groups, followed by Bacteroidetes and Firmicutes. Spearman and redundancy analyses demonstrated that an increase in the relative abundance of beneficial bacteria may stimulate the antioxidant system to defend against exogenous pollution. Our study provides valuable information about the combined toxicological effects of TC and MW on the growth of medicinal plants.
{"title":"Combined impacts of tetracycline and multi-walled carbon nanotubes on the growth of Chrysanthemum coronarium L. and its root environment","authors":"Minling Gao, Hongchang Peng, Zhengzhen Xiao, Ling Xiao, Youming Dong, Iranzi Emile RUSHIMISHA Emile RUSHIMISHA, Wenhao Yu, Zhengguo Song","doi":"10.1039/d4en00790e","DOIUrl":"https://doi.org/10.1039/d4en00790e","url":null,"abstract":"Multi-walled carbon nanotubes (MW) and tetracycline (TC) usually co-occur in the environment and can threaten plant growth. However, little is known about their combined effect on plant growth. We conducted hydroponic experiments to investigate the uptake, transport, and compartmentalization of TC in Chrysanthemum coronarium L. in the presence of MW, and the combined effects of TC and MW on the root environment were also studied. The results revealed that the presence of MW reduced the concentration of TC in the four subcellular fractions, both in the leaves and roots, compared with TC alone. Co-pollution with TC and MW stimulated the production and accumulation of superoxide anions (O2.-), and hydrogen peroxide (H2O2) in the leaves and roots compared to TC alone, leading to an increase in the malondialdehyde content; this inhibited photosynthesis by reducing the activity of ribulose-1,5-bisphosphate carboxylase and dehydrogenase activity, resulting in a decrease in the dry weight of leaves and roots. The increased O2.- and H2O2 contents induced superoxide dismutase and catalase activities to alleviate oxidative damage. In addition, compared with single TC contamination, the co-application of TC and MW significantly increased the concentrations of oxalic acid and formic acid in root secretions, stimulated the activity of microorganisms, and improved autochthonous input and humification of dissolved organic matter in the growth medium. High-throughput sequencing revealed that Proteobacteria were the dominant bacteria in the medium solution across all groups, followed by Bacteroidetes and Firmicutes. Spearman and redundancy analyses demonstrated that an increase in the relative abundance of beneficial bacteria may stimulate the antioxidant system to defend against exogenous pollution. Our study provides valuable information about the combined toxicological effects of TC and MW on the growth of medicinal plants.","PeriodicalId":73,"journal":{"name":"Environmental Science: Nano","volume":"3 1","pages":""},"PeriodicalIF":8.131,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143776111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Qianqian Yang, Xiangyi Hou, Feng Lu, Dahai Zhang, Wentao Lin, Nick Schlensky, Zhixiang Chen, Yan Zhang, Xuzhi Zhang
The potential threat that silver nanoparticles (Ag NPs) pose to bacterial communities in estuarine environments has become a subject of intensifying global interest. Herein, eight water samples were collected from various estuarine sites. They were characterized by a wide array of distinct physicochemical properties, including pH, salinity, conductivity, turbidity, chemical oxygen demand (COD) and total suspended solids (TSS). Vibrio parahaemolyticus (V. parahaemolyticus) were exposed to Ag NPs at a series of concentrations in these water samples. Subsequently, the growth curves of the surviving bacterial cells were measured using an electronic microbial growth analyzer to determine the minimum inhibitory concentrations (MICs) of Ag NPs against V. parahaemolyticus. The results revealed a remarkable variation in the MICs, with values ranging from 12.0 mg/L to > 48.0 mg/L. A comprehensive analysis indicated that there were no clear and definitive relationships between the MIC and individual physicochemical parameters such as pH, salinity, conductivity, turbidity, COD and TSS. Instead, the adverse effect of Ag NPs on V. parahaemolyticus depended on the combination of these factors. In contrast, the MIC Ag NPs against V. parahaemolyticus in physiological saline, a commonly used simple laboratory medium, was determined to be 6.0 mg/L, which was significantly lower compared to those observed in the estuarine water samples. Therefore, when assessing the ecotoxicity of Ag NPs in actual estuarine scenarios, it is essential to ground on the antimicrobial data collected directly from realistic environmental matrices, rather than relying on data obtained from simple laboratory media or so-called representative water samples.
{"title":"Evidently diverse effects of silver nanoparticles on Vibrio parahaemolyticus across different estuarine water samples","authors":"Qianqian Yang, Xiangyi Hou, Feng Lu, Dahai Zhang, Wentao Lin, Nick Schlensky, Zhixiang Chen, Yan Zhang, Xuzhi Zhang","doi":"10.1039/d5en00018a","DOIUrl":"https://doi.org/10.1039/d5en00018a","url":null,"abstract":"The potential threat that silver nanoparticles (Ag NPs) pose to bacterial communities in estuarine environments has become a subject of intensifying global interest. Herein, eight water samples were collected from various estuarine sites. They were characterized by a wide array of distinct physicochemical properties, including pH, salinity, conductivity, turbidity, chemical oxygen demand (COD) and total suspended solids (TSS). Vibrio parahaemolyticus (V. parahaemolyticus) were exposed to Ag NPs at a series of concentrations in these water samples. Subsequently, the growth curves of the surviving bacterial cells were measured using an electronic microbial growth analyzer to determine the minimum inhibitory concentrations (MICs) of Ag NPs against V. parahaemolyticus. The results revealed a remarkable variation in the MICs, with values ranging from 12.0 mg/L to > 48.0 mg/L. A comprehensive analysis indicated that there were no clear and definitive relationships between the MIC and individual physicochemical parameters such as pH, salinity, conductivity, turbidity, COD and TSS. Instead, the adverse effect of Ag NPs on V. parahaemolyticus depended on the combination of these factors. In contrast, the MIC Ag NPs against V. parahaemolyticus in physiological saline, a commonly used simple laboratory medium, was determined to be 6.0 mg/L, which was significantly lower compared to those observed in the estuarine water samples. Therefore, when assessing the ecotoxicity of Ag NPs in actual estuarine scenarios, it is essential to ground on the antimicrobial data collected directly from realistic environmental matrices, rather than relying on data obtained from simple laboratory media or so-called representative water samples.","PeriodicalId":73,"journal":{"name":"Environmental Science: Nano","volume":"58 1","pages":""},"PeriodicalIF":8.131,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143758363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Veronique Collin-Faure, Aliro Villacorta, Marianne Vitipon, Helene Diemer, Sarah Cianferani, Ricardo Marcos, Elisabeth Darrouzet, Alba Hernández Bonilla, Thierry Rabilloud
Plastics are emerging pollutants of great concern. Macroplastics degrade into microplastics and nanoplastics, which can accumulate in living organisms with still poorly known consequences. Nanoplastics being particulate pollutants, they are handled in animal organisms by scavenger cells such as macrophages, which are important players in the immune system. Polyethylene terephthalate is one of these plastics of concern, as it is widely used in food packaging where it releases nanoparticles. We have thus undertaken a study on the effects of true-to-life polyethylene terephthalate nanoparticles prepared from water bottles on macrophages. To this purpose, we used a combination of proteomics and targeted validation experiments. Proteomics showed important adaptive changes in the proteome in response to exposure to polyethylene terephthalate nanoparticles. These changes affected for example mitochondrial, cytoskeletal and lysosomal proteins, but also proteins implicated in immune functions. Validation experiments showed that many of these changes were homeostatic, with no induced oxidative stress and no gross perturbation of the mitochondrial function. However, polyethylene terephthalate nanoparticles induced endoplasmic reticulum stress and disturbed the immune functions of macrophages. We indeed observed a slight pro-inflammatory response (1.5-fold increase in TNF secretion). We also observed a decrease in the response to bacterial stimulation (1.6 decrease in IL-6 secretion). We also observed a 20 percent decrease in the expression of important proteins involved in immune responses such as TLR2, TLR7 or collectin 12, and a two-fold decrease in the production of lysozyme. This suggests that macrophages having ingested polyethylene terephthalate nanoparticles are less efficient in their immune functions.
{"title":"About the effects of true to life polyethylene terephthalate nanoparticles on macrophages.","authors":"Veronique Collin-Faure, Aliro Villacorta, Marianne Vitipon, Helene Diemer, Sarah Cianferani, Ricardo Marcos, Elisabeth Darrouzet, Alba Hernández Bonilla, Thierry Rabilloud","doi":"10.1039/d4en01063a","DOIUrl":"https://doi.org/10.1039/d4en01063a","url":null,"abstract":"Plastics are emerging pollutants of great concern. Macroplastics degrade into microplastics and nanoplastics, which can accumulate in living organisms with still poorly known consequences. Nanoplastics being particulate pollutants, they are handled in animal organisms by scavenger cells such as macrophages, which are important players in the immune system. Polyethylene terephthalate is one of these plastics of concern, as it is widely used in food packaging where it releases nanoparticles. We have thus undertaken a study on the effects of true-to-life polyethylene terephthalate nanoparticles prepared from water bottles on macrophages. To this purpose, we used a combination of proteomics and targeted validation experiments. Proteomics showed important adaptive changes in the proteome in response to exposure to polyethylene terephthalate nanoparticles. These changes affected for example mitochondrial, cytoskeletal and lysosomal proteins, but also proteins implicated in immune functions. Validation experiments showed that many of these changes were homeostatic, with no induced oxidative stress and no gross perturbation of the mitochondrial function. However, polyethylene terephthalate nanoparticles induced endoplasmic reticulum stress and disturbed the immune functions of macrophages. We indeed observed a slight pro-inflammatory response (1.5-fold increase in TNF secretion). We also observed a decrease in the response to bacterial stimulation (1.6 decrease in IL-6 secretion). We also observed a 20 percent decrease in the expression of important proteins involved in immune responses such as TLR2, TLR7 or collectin 12, and a two-fold decrease in the production of lysozyme. This suggests that macrophages having ingested polyethylene terephthalate nanoparticles are less efficient in their immune functions.","PeriodicalId":73,"journal":{"name":"Environmental Science: Nano","volume":"131 1","pages":""},"PeriodicalIF":8.131,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143744726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lukas Wimmer, My Vanessa Nguyen Hoang, Jacqueline Schwarzinger, Vesna B Jovanović, Boban Anđelković, Tanja Cirkovic Velickovic, Thomas Meisel, Tassilo Waniek, Christiane Weimann, Korinna Altmann, Lea Ann Dailey
Micro- and nanoplastics have become environmental pollutants of concern, receiving increased attention from consumers, scientists, and policymakers. The lack of knowledge about possible impacts on wildlife and human health requires further research, for which well-characterized test materials are needed. A quality-by-design (QbD) driven approach was used to produce sterile, endotoxin monitored nanoplastics of polyethylene terephthalate (PET) and polypropylene (PP) with a size fraction of > 90% below 1 µm and high yield of > 90%. Glycerol was used as a versatile and biocompatible liquid storage medium which requires no further exogenous dispersing agent and maintained colloidal stability, sterility (0 CFU/mL), and low endotoxin levels (< 0.1 EU/mL) for more than one year of storage at room temperature. Further, the glycerol vehicle showed no biological effect on the tested human bronchial cell line Calu-3 up to 0.8% (w/v). Given the concentration of 40 mg/g nanoplastics in the glycerol stock, this corresponds to a nanoplastic concentration of 320 µg/mL. The surfactant-free nanoplastics are dispersible in bio-relevant media from the glycerol stock without changing size characteristics and are suitable for in vitro and in vivo research.
{"title":"A quality-by-design inspired approach to develop PET and PP nanoplastic test materials for use in in vitro and in vivo biological assays","authors":"Lukas Wimmer, My Vanessa Nguyen Hoang, Jacqueline Schwarzinger, Vesna B Jovanović, Boban Anđelković, Tanja Cirkovic Velickovic, Thomas Meisel, Tassilo Waniek, Christiane Weimann, Korinna Altmann, Lea Ann Dailey","doi":"10.1039/d4en01186d","DOIUrl":"https://doi.org/10.1039/d4en01186d","url":null,"abstract":"Micro- and nanoplastics have become environmental pollutants of concern, receiving increased attention from consumers, scientists, and policymakers. The lack of knowledge about possible impacts on wildlife and human health requires further research, for which well-characterized test materials are needed. A quality-by-design (QbD) driven approach was used to produce sterile, endotoxin monitored nanoplastics of polyethylene terephthalate (PET) and polypropylene (PP) with a size fraction of > 90% below 1 µm and high yield of > 90%. Glycerol was used as a versatile and biocompatible liquid storage medium which requires no further exogenous dispersing agent and maintained colloidal stability, sterility (0 CFU/mL), and low endotoxin levels (< 0.1 EU/mL) for more than one year of storage at room temperature. Further, the glycerol vehicle showed no biological effect on the tested human bronchial cell line Calu-3 up to 0.8% (w/v). Given the concentration of 40 mg/g nanoplastics in the glycerol stock, this corresponds to a nanoplastic concentration of 320 µg/mL. The surfactant-free nanoplastics are dispersible in bio-relevant media from the glycerol stock without changing size characteristics and are suitable for in vitro and in vivo research.","PeriodicalId":73,"journal":{"name":"Environmental Science: Nano","volume":"36 1","pages":""},"PeriodicalIF":8.131,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143744727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Peng Lin, Jinglin Yuan, Mingyu Lei, Yi Jin, Sikai Chen, Han Zhang
In response to the growing focus on bio-waste valorization, this study extracted cellulose fiber from rice straw, coffee grounds, corncob, and rape straw. The extracted fiber was subsequently cationically modified into cellulose nanofibers (QCNF) for effective flocculation in high-turbidity wastewater treatment. Scanning electron microscopy (SEM) revealed that the QCNF derived from the four biomass sources displayed a three-dimensional network structure, with each displaying distinct fiber morphologies. The QCNF derived from coffee grounds exhibited a flaky structure, the QCNF derived from corncob displayed short, rod-like fibers, and the QCNF derived from rice straw and rape straw showed chain-like structures, with rice straw fibers appearing particularly attenuated. The results indicate significant variation in the flocculation efficiency of QCNF derived from different bio-sources. QCNF prepared from rice straw exhibited the highest flocculation efficiency, achieving a removal rate of 90.6%. SEM analysis of the physical structure and morphology of the four types of QCNF revealed that nanofibers with slender chain-like structures are particularly well-suited for developing biomass-based flocculants.
{"title":"Removal of suspended solids from water by waste biomass-based cationized cellulose nanofibers: a comparative analysis of flocculation performance from different biomass sources","authors":"Peng Lin, Jinglin Yuan, Mingyu Lei, Yi Jin, Sikai Chen, Han Zhang","doi":"10.1039/d4en01120a","DOIUrl":"https://doi.org/10.1039/d4en01120a","url":null,"abstract":"In response to the growing focus on bio-waste valorization, this study extracted cellulose fiber from rice straw, coffee grounds, corncob, and rape straw. The extracted fiber was subsequently cationically modified into cellulose nanofibers (QCNF) for effective flocculation in high-turbidity wastewater treatment. Scanning electron microscopy (SEM) revealed that the QCNF derived from the four biomass sources displayed a three-dimensional network structure, with each displaying distinct fiber morphologies. The QCNF derived from coffee grounds exhibited a flaky structure, the QCNF derived from corncob displayed short, rod-like fibers, and the QCNF derived from rice straw and rape straw showed chain-like structures, with rice straw fibers appearing particularly attenuated. The results indicate significant variation in the flocculation efficiency of QCNF derived from different bio-sources. QCNF prepared from rice straw exhibited the highest flocculation efficiency, achieving a removal rate of 90.6%. SEM analysis of the physical structure and morphology of the four types of QCNF revealed that nanofibers with slender chain-like structures are particularly well-suited for developing biomass-based flocculants.","PeriodicalId":73,"journal":{"name":"Environmental Science: Nano","volume":"30 1","pages":""},"PeriodicalIF":8.131,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143744728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Natalia Juica, Gonzalo Bustos, Sindy Devis, Carolina Klagges, Nicolas Oneto, Jeffri S. Retamal, Luis Constandil
The environmental pollution and health risks associated with synthetic pesticides have driven increasing interest in plant-derived biopesticides like geraniol. However, their practical application is limited by high volatility and low solubility. In this study, lignin nanoparticles were used as a carrier system to enhance the stability and acaricidal efficacy of geraniol against Brevipalpus chilensis. The nanoprecipitation process enabled the synthesis of spherical geraniol-loaded lignin nanoparticles with an average size of 200 ± 27.2 nm, a surface charge of −29± 3.9 mV and an encapsulation efficiency of 46.5%. The release profile of encapsulated geraniol was assessed, and UV exposure assays demonstrated significantly improved stability compared to free geraniol. Bioassays revealed significantly higher mortality rates of Brevipalpus chilensis when treated with geraniol-loaded nanoparticles compared to free geraniol, highlighting the enhanced efficacy of the encapsulated compound. Additionally, nanoparticle formulations exhibited low cytotoxicity in HeLa cells. Overall, this study underscores the potential of lignin nanoparticles as a promising delivery system for optimizing biopesticide formulations in sustainable agriculture
{"title":"Acaricidal activity of geraniol-loaded lignin nanoparticles for the control of Brevipalpus chilensis: an eco-friendly approach to crop protection","authors":"Natalia Juica, Gonzalo Bustos, Sindy Devis, Carolina Klagges, Nicolas Oneto, Jeffri S. Retamal, Luis Constandil","doi":"10.1039/d5en00155b","DOIUrl":"https://doi.org/10.1039/d5en00155b","url":null,"abstract":"The environmental pollution and health risks associated with synthetic pesticides have driven increasing interest in plant-derived biopesticides like geraniol. However, their practical application is limited by high volatility and low solubility. In this study, lignin nanoparticles were used as a carrier system to enhance the stability and acaricidal efficacy of geraniol against Brevipalpus chilensis. The nanoprecipitation process enabled the synthesis of spherical geraniol-loaded lignin nanoparticles with an average size of 200 ± 27.2 nm, a surface charge of −29± 3.9 mV and an encapsulation efficiency of 46.5%. The release profile of encapsulated geraniol was assessed, and UV exposure assays demonstrated significantly improved stability compared to free geraniol. Bioassays revealed significantly higher mortality rates of Brevipalpus chilensis when treated with geraniol-loaded nanoparticles compared to free geraniol, highlighting the enhanced efficacy of the encapsulated compound. Additionally, nanoparticle formulations exhibited low cytotoxicity in HeLa cells. Overall, this study underscores the potential of lignin nanoparticles as a promising delivery system for optimizing biopesticide formulations in sustainable agriculture","PeriodicalId":73,"journal":{"name":"Environmental Science: Nano","volume":"31 1","pages":""},"PeriodicalIF":8.131,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143723241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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