Pub Date : 2022-10-11DOI: 10.1088/2516-1091/ac993d
F. Iacopi, Chin-Teng Lin
This Perspective offers a concise overview of the current, state-of-the-art, neural sensors for brain-machine interfaces, with particular attention towards brain-controlled robotics. We first describe current approaches, decoding models and associated choice of common paradigms, and their relation to the position and requirements of the neural sensors. While implanted intracortical sensors offer unparalleled spatial, temporal and frequency resolution, the risks related to surgery and post-surgery complications pose a significant barrier to deployment beyond severely disabled individuals. For less critical and larger scale applications, we emphasize the need to further develop dry scalp electroencephalography (EEG) sensors as non-invasive probes with high sensitivity, accuracy, comfort and robustness for prolonged and repeated use. In particular, as many of the employed paradigms require placing EEG sensors in hairy areas of the scalp, ensuring the aforementioned requirements becomes particularly challenging. Nevertheless, neural sensing technologies in this area are accelerating thanks to the advancement of miniaturised technologies and the engineering of novel biocompatible nanomaterials. The development of novel multifunctional nanomaterials is also expected to enable the integration of redundancy by probing the same type of information through different mechanisms for increased accuracy, as well as the integration of complementary and synergetic functions that could range from the monitoring of physiological states to incorporating optical imaging.
{"title":"A perspective on electroencephalography sensors for brain-computer interfaces","authors":"F. Iacopi, Chin-Teng Lin","doi":"10.1088/2516-1091/ac993d","DOIUrl":"https://doi.org/10.1088/2516-1091/ac993d","url":null,"abstract":"This Perspective offers a concise overview of the current, state-of-the-art, neural sensors for brain-machine interfaces, with particular attention towards brain-controlled robotics. We first describe current approaches, decoding models and associated choice of common paradigms, and their relation to the position and requirements of the neural sensors. While implanted intracortical sensors offer unparalleled spatial, temporal and frequency resolution, the risks related to surgery and post-surgery complications pose a significant barrier to deployment beyond severely disabled individuals. For less critical and larger scale applications, we emphasize the need to further develop dry scalp electroencephalography (EEG) sensors as non-invasive probes with high sensitivity, accuracy, comfort and robustness for prolonged and repeated use. In particular, as many of the employed paradigms require placing EEG sensors in hairy areas of the scalp, ensuring the aforementioned requirements becomes particularly challenging. Nevertheless, neural sensing technologies in this area are accelerating thanks to the advancement of miniaturised technologies and the engineering of novel biocompatible nanomaterials. The development of novel multifunctional nanomaterials is also expected to enable the integration of redundancy by probing the same type of information through different mechanisms for increased accuracy, as well as the integration of complementary and synergetic functions that could range from the monitoring of physiological states to incorporating optical imaging.","PeriodicalId":74582,"journal":{"name":"Progress in biomedical engineering (Bristol, England)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44540323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-21DOI: 10.1088/2516-1091/ac93d3
R. Rai, J. Roether, A. Boccaccini
A number of electrically conducting polymers, such as polyaniline (PANi), as well as functionalized aniline copolymers and composites, which are simultaneously biodegradable and conductive, have been applied for developing electrically conductive scaffolds for tissue engineering (TE) in recent years. The rationale behind these scaffolds is to induce ‘electroactivity’ in scaffolds, as many research works have shown that an intrinsic electrical activity leads to both increased regeneration rates and improved healing of damaged tissues. PANi is the conductive polymer of choice because it is economical and easy to process with a variety of methods. The resultant PANi based biomaterials have shown biocompatibility, conductivity, suitable processability, positive cellular response, as well as an intrinsic antibacterial effect in numerous research studies. The analysis of the literature has revealed that PANi based scaffolds have been investigated for TE applications including skin/wound healing, bone, cartilage, nerve/spinal cord, vascular, skeletal muscle repair and for the treatment of infertility. Although PANi based materials find widespread applications in other sectors, they are still far away from being commercially exploited as scaffolds for TE despite positive research results. This review aims to discuss and critically assess the current state of PANi based TE scaffolds for different applications. A future perspective for utilizing PANi based biomaterials for applications in TE is discussed, including recent considerations about potential cytotoxic effects.
{"title":"Polyaniline based polymers in tissue engineering applications: a review","authors":"R. Rai, J. Roether, A. Boccaccini","doi":"10.1088/2516-1091/ac93d3","DOIUrl":"https://doi.org/10.1088/2516-1091/ac93d3","url":null,"abstract":"A number of electrically conducting polymers, such as polyaniline (PANi), as well as functionalized aniline copolymers and composites, which are simultaneously biodegradable and conductive, have been applied for developing electrically conductive scaffolds for tissue engineering (TE) in recent years. The rationale behind these scaffolds is to induce ‘electroactivity’ in scaffolds, as many research works have shown that an intrinsic electrical activity leads to both increased regeneration rates and improved healing of damaged tissues. PANi is the conductive polymer of choice because it is economical and easy to process with a variety of methods. The resultant PANi based biomaterials have shown biocompatibility, conductivity, suitable processability, positive cellular response, as well as an intrinsic antibacterial effect in numerous research studies. The analysis of the literature has revealed that PANi based scaffolds have been investigated for TE applications including skin/wound healing, bone, cartilage, nerve/spinal cord, vascular, skeletal muscle repair and for the treatment of infertility. Although PANi based materials find widespread applications in other sectors, they are still far away from being commercially exploited as scaffolds for TE despite positive research results. This review aims to discuss and critically assess the current state of PANi based TE scaffolds for different applications. A future perspective for utilizing PANi based biomaterials for applications in TE is discussed, including recent considerations about potential cytotoxic effects.","PeriodicalId":74582,"journal":{"name":"Progress in biomedical engineering (Bristol, England)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48599945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-13DOI: 10.1088/2516-1091/ac91b6
G. Severini, M. Zych
The term ‘locomotor adaptations’ (LMA) indicates the alteration in motor commands that is automatically or volitionally generated in response to a perturbation continuously altering the task demands of locomotion. LMAs have been widely studied, using a variety of experimental paradigms and analysis techniques. The perturbation can be expected or unexpected and constituted by a change in the movement environment, by forces actively pushing the person’s body segments, by a modification in the sensory feedback associated with the task or by explicit task instructions. The study of LMAs has been key in widening our understanding of the principles regulating bipedal locomotion, from the overall strategies driving the short-term adjustments of motor commands, down to the different neural circuits involved in the different aspects of locomotion. In this paper we will provide an in-depth review of the research field of LMAs. We will start with an analysis of the principles driving the evolution of bipedal locomotion in humans. Then we will review the different experimental paradigms that have been used to trigger LMAs. We will analyze the evidence on the neurophysiological correlates of adaptation and the behavioral reasons behind it. We will then discuss the characteristics of LMA such as transfer, generalization, and savings. This will be followed by a critical analysis of how different studies point to different task-goal related drivers of adaptation. Finally, we will conclude with a perspective on the research field of LMAs and on its ramifications in neuroscience and rehabilitation.
{"title":"Locomotor adaptations: paradigms, principles and perspectives","authors":"G. Severini, M. Zych","doi":"10.1088/2516-1091/ac91b6","DOIUrl":"https://doi.org/10.1088/2516-1091/ac91b6","url":null,"abstract":"The term ‘locomotor adaptations’ (LMA) indicates the alteration in motor commands that is automatically or volitionally generated in response to a perturbation continuously altering the task demands of locomotion. LMAs have been widely studied, using a variety of experimental paradigms and analysis techniques. The perturbation can be expected or unexpected and constituted by a change in the movement environment, by forces actively pushing the person’s body segments, by a modification in the sensory feedback associated with the task or by explicit task instructions. The study of LMAs has been key in widening our understanding of the principles regulating bipedal locomotion, from the overall strategies driving the short-term adjustments of motor commands, down to the different neural circuits involved in the different aspects of locomotion. In this paper we will provide an in-depth review of the research field of LMAs. We will start with an analysis of the principles driving the evolution of bipedal locomotion in humans. Then we will review the different experimental paradigms that have been used to trigger LMAs. We will analyze the evidence on the neurophysiological correlates of adaptation and the behavioral reasons behind it. We will then discuss the characteristics of LMA such as transfer, generalization, and savings. This will be followed by a critical analysis of how different studies point to different task-goal related drivers of adaptation. Finally, we will conclude with a perspective on the research field of LMAs and on its ramifications in neuroscience and rehabilitation.","PeriodicalId":74582,"journal":{"name":"Progress in biomedical engineering (Bristol, England)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46783509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-30DOI: 10.1088/2516-1091/ac8dcf
Sònia Sabaté-Soler, M. Bernini, J. Schwamborn
Microglia, the immune cells of the brain, are a focus of studies in neurodegenerative diseases. Similarly, research about induced pluripotent stem cell (iPSC)-derived whole brain and region-specific organoids is increasing. In organoids, the complexity of the culture systems increases, mimicking better the actual scenario in the human brain. Furthermore, animal models do not always recapitulate human neurodegeneration, and they imply more ethical concerns compared to organoid systems. Recently the integration of iPSC-derived microglia into brain organoids has been achieved, and on-chip technologies have been focusing on microglia interaction with neural cells. In this review, we discuss the achievements on integrating microglia into brain organoids. We study the cell organization, ultrastructure and cell signalling of microglia with respect to other cell types in organoids as well as their functionality in the system. A particular focus here is on the interaction with the midbrain and dopaminergic systems. Finally, we discuss the achievements until now concerning neuroinflammation and disease modelling, and the possible therapeutic approaches targeting microglia and neuroinflammation in 3D systems.
{"title":"Immunocompetent brain organoids—microglia enter the stage","authors":"Sònia Sabaté-Soler, M. Bernini, J. Schwamborn","doi":"10.1088/2516-1091/ac8dcf","DOIUrl":"https://doi.org/10.1088/2516-1091/ac8dcf","url":null,"abstract":"Microglia, the immune cells of the brain, are a focus of studies in neurodegenerative diseases. Similarly, research about induced pluripotent stem cell (iPSC)-derived whole brain and region-specific organoids is increasing. In organoids, the complexity of the culture systems increases, mimicking better the actual scenario in the human brain. Furthermore, animal models do not always recapitulate human neurodegeneration, and they imply more ethical concerns compared to organoid systems. Recently the integration of iPSC-derived microglia into brain organoids has been achieved, and on-chip technologies have been focusing on microglia interaction with neural cells. In this review, we discuss the achievements on integrating microglia into brain organoids. We study the cell organization, ultrastructure and cell signalling of microglia with respect to other cell types in organoids as well as their functionality in the system. A particular focus here is on the interaction with the midbrain and dopaminergic systems. Finally, we discuss the achievements until now concerning neuroinflammation and disease modelling, and the possible therapeutic approaches targeting microglia and neuroinflammation in 3D systems.","PeriodicalId":74582,"journal":{"name":"Progress in biomedical engineering (Bristol, England)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41609811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-16DOI: 10.1088/2516-1091/ac8a2f
S. Maharjan, Diana Priscilla Bonilla-Ruelas, G. Orive, Y. S. Zhang
As the field of tissue engineering and regenerative medicine progresses, the possibility for artificial organs to restore normal tissue functions seems to become more feasible. However, a major challenge in the long-term culture of the engineered tissues is the lack of adequate oxygenation. The photosynthetic supply of oxygen (O2) for tissues and organs using photoautotrophic microorganisms has been explored recently in both in vitro and in vivo studies. The biofabrication of photosymbiotic scaffolds using biomaterials, photosynthetic microorganisms, and human cells has shown constant generation of O2 in response to light illumination while avoiding hypoxic conditions. This emerging strategy of photosymbiotic oxygenation is potentially an attractive approach to overcome the need of adequate oxygenation in tissue engineering and regenerative medicine. This Perspective aims to present an overview on the applications of photoautotrophic microorganism-enabled oxygenation strategies for overcoming hypoxia-related challenges in tissue engineering and regenerative medicine.
{"title":"Photosymbiotic tissue engineering and regeneration","authors":"S. Maharjan, Diana Priscilla Bonilla-Ruelas, G. Orive, Y. S. Zhang","doi":"10.1088/2516-1091/ac8a2f","DOIUrl":"https://doi.org/10.1088/2516-1091/ac8a2f","url":null,"abstract":"As the field of tissue engineering and regenerative medicine progresses, the possibility for artificial organs to restore normal tissue functions seems to become more feasible. However, a major challenge in the long-term culture of the engineered tissues is the lack of adequate oxygenation. The photosynthetic supply of oxygen (O2) for tissues and organs using photoautotrophic microorganisms has been explored recently in both in vitro and in vivo studies. The biofabrication of photosymbiotic scaffolds using biomaterials, photosynthetic microorganisms, and human cells has shown constant generation of O2 in response to light illumination while avoiding hypoxic conditions. This emerging strategy of photosymbiotic oxygenation is potentially an attractive approach to overcome the need of adequate oxygenation in tissue engineering and regenerative medicine. This Perspective aims to present an overview on the applications of photoautotrophic microorganism-enabled oxygenation strategies for overcoming hypoxia-related challenges in tissue engineering and regenerative medicine.","PeriodicalId":74582,"journal":{"name":"Progress in biomedical engineering (Bristol, England)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42055266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-07-19DOI: 10.1088/2516-1091/ac8259
Z. Baka, Marie Stiefel, Agathe Figarol, Claire Godier, Abhik Mallick, O. Joubert, N. Ashammakhi, E. Gaffet, H. Alem
Conventional 2D cell cultures are widely used for the development of new anticancer drugs. However, their relevance as in vitro models is increasingly questioned as they are considered too simplistic compared to complex, three-dimensional in vivo tumors. Moreover, animal experiments are not only costly and time-consuming, but also raise ethical issues and their use for some applications has been restricted. Therefore, it becomes crucial to develop new experimental models that better capture the complexity and dynamic aspects of in vivo tumors. New approaches based on microfluidic technology are promising. This technology has indeed been used to create microphysiological systems called ‘organ-on-chip’ which simulate key structural and functional features of human tissues and organs. These devices have further been adapted to create cancer models giving rise to the ‘cancer-on-chip’ (COC) concept. In this review, we will discuss the main COC models described so far for major cancer types including lung, prostate, breast, colorectal, pancreatic, and ovarian cancers. Then, we will highlight the challenges that this technology is facing and the possible research perspectives that can arise from them.
{"title":"Cancer-on-chip technology: current applications in major cancer types, challenges and future prospects","authors":"Z. Baka, Marie Stiefel, Agathe Figarol, Claire Godier, Abhik Mallick, O. Joubert, N. Ashammakhi, E. Gaffet, H. Alem","doi":"10.1088/2516-1091/ac8259","DOIUrl":"https://doi.org/10.1088/2516-1091/ac8259","url":null,"abstract":"Conventional 2D cell cultures are widely used for the development of new anticancer drugs. However, their relevance as in vitro models is increasingly questioned as they are considered too simplistic compared to complex, three-dimensional in vivo tumors. Moreover, animal experiments are not only costly and time-consuming, but also raise ethical issues and their use for some applications has been restricted. Therefore, it becomes crucial to develop new experimental models that better capture the complexity and dynamic aspects of in vivo tumors. New approaches based on microfluidic technology are promising. This technology has indeed been used to create microphysiological systems called ‘organ-on-chip’ which simulate key structural and functional features of human tissues and organs. These devices have further been adapted to create cancer models giving rise to the ‘cancer-on-chip’ (COC) concept. In this review, we will discuss the main COC models described so far for major cancer types including lung, prostate, breast, colorectal, pancreatic, and ovarian cancers. Then, we will highlight the challenges that this technology is facing and the possible research perspectives that can arise from them.","PeriodicalId":74582,"journal":{"name":"Progress in biomedical engineering (Bristol, England)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44745800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-07-15DOI: 10.1088/2516-1091/ac8193
Yanan Li, Aran Sena, Ziwei Wang, Xueyan Xing, J. Babič, E. V. van Asseldonk, E. Burdet
Interaction control presents opportunities for contact robots physically interacting with their human user, such as assistance targeted to each human user, communication of goals to enable effective teamwork, and task-directed motion resistance in physical training and rehabilitation contexts. Here we review the burgeoning field of interaction control in the control theory and machine learning communities, by analysing the exchange of haptic information between the robot and its human user, and how they share the task effort. We first review the estimation and learning methods to predict the human user intent with the large uncertainty, variability and noise and limited observation of human motion. Based on this motion intent core, typical interaction control strategies are described using a homotopy of shared control parameters. Recent methods of haptic communication and game theory are then presented to consider the co-adaptation of human and robot control and yield versatile interactive control as observed between humans. Finally, the limitations of the presented state of the art are discussed and directions for future research are outlined.
{"title":"A review on interaction control for contact robots through intent detection","authors":"Yanan Li, Aran Sena, Ziwei Wang, Xueyan Xing, J. Babič, E. V. van Asseldonk, E. Burdet","doi":"10.1088/2516-1091/ac8193","DOIUrl":"https://doi.org/10.1088/2516-1091/ac8193","url":null,"abstract":"Interaction control presents opportunities for contact robots physically interacting with their human user, such as assistance targeted to each human user, communication of goals to enable effective teamwork, and task-directed motion resistance in physical training and rehabilitation contexts. Here we review the burgeoning field of interaction control in the control theory and machine learning communities, by analysing the exchange of haptic information between the robot and its human user, and how they share the task effort. We first review the estimation and learning methods to predict the human user intent with the large uncertainty, variability and noise and limited observation of human motion. Based on this motion intent core, typical interaction control strategies are described using a homotopy of shared control parameters. Recent methods of haptic communication and game theory are then presented to consider the co-adaptation of human and robot control and yield versatile interactive control as observed between humans. Finally, the limitations of the presented state of the art are discussed and directions for future research are outlined.","PeriodicalId":74582,"journal":{"name":"Progress in biomedical engineering (Bristol, England)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48572779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-07-14DOI: 10.1088/2516-1091/ac812c
Eleonora Borda, D. Ghezzi
Vision is an extraordinary sense through which we can appreciate the beauty of the world we live in, gain invaluable knowledge and communicate with others using visual expression and arts. On the contrary, blindness is a threatening medical condition disrupting the life of affected people and their families. Therefore, restoring sight is one of the open challenges of our society. Today, the synergistic convergence of science and technology holds the potential to provide blind patients with artificial vision using visual prostheses: a type of implantable medical device able to reactivate visual neurons using electrical stimulation. Although clinical trials showed that vision restoration is still far away, significant technological advances make visual prostheses a valuable solution for blind patients. This review is not only a description of the state-of-the-art. Instead, it provides the reader with an update on recent developments, a critical discussion of the open challenges, and an overview of promising future directions.
{"title":"Advances in visual prostheses: engineering and biological challenges","authors":"Eleonora Borda, D. Ghezzi","doi":"10.1088/2516-1091/ac812c","DOIUrl":"https://doi.org/10.1088/2516-1091/ac812c","url":null,"abstract":"Vision is an extraordinary sense through which we can appreciate the beauty of the world we live in, gain invaluable knowledge and communicate with others using visual expression and arts. On the contrary, blindness is a threatening medical condition disrupting the life of affected people and their families. Therefore, restoring sight is one of the open challenges of our society. Today, the synergistic convergence of science and technology holds the potential to provide blind patients with artificial vision using visual prostheses: a type of implantable medical device able to reactivate visual neurons using electrical stimulation. Although clinical trials showed that vision restoration is still far away, significant technological advances make visual prostheses a valuable solution for blind patients. This review is not only a description of the state-of-the-art. Instead, it provides the reader with an update on recent developments, a critical discussion of the open challenges, and an overview of promising future directions.","PeriodicalId":74582,"journal":{"name":"Progress in biomedical engineering (Bristol, England)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47052480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-13DOI: 10.1088/2516-1091/ac7833
Defne Yigci, M. R. Sarabi, M. Ustun, Nazente Atçeken, Emel Sokullu, T. Bagci-Onder, S. Tasoglu
Glioma is one of the most malignant types of cancer and most gliomas remain incurable. One of the hallmarks of glioma is its invasiveness. Furthermore, glioma cells tend to readily detach from the primary tumor and travel through the brain tissue, making complete tumor resection impossible in many cases. To expand the knowledge regarding the invasive behavior of glioma, evaluate drug resistance, and recapitulate the tumor microenvironment, various modeling strategies were proposed in the last decade, including three-dimensional (3D) biomimetic scaffold-free cultures, organ-on-chip microfluidics chips, and 3D bioprinting platforms, which allow for the investigation on patient-specific treatments. The emerging method of 3D bioprinting technology has introduced a time- and cost-efficient approach to create in vitro models that possess the structural and functional characteristics of human organs and tissues by spatially positioning cells and bioink. Here, we review emerging 3D bioprinted models developed for recapitulating the brain environment and glioma tumors, with the purpose of probing glioma cell invasion and gliomagenesis and discuss the potential use of 4D printing and machine learning applications in glioma modelling.
{"title":"3D bioprinted glioma models","authors":"Defne Yigci, M. R. Sarabi, M. Ustun, Nazente Atçeken, Emel Sokullu, T. Bagci-Onder, S. Tasoglu","doi":"10.1088/2516-1091/ac7833","DOIUrl":"https://doi.org/10.1088/2516-1091/ac7833","url":null,"abstract":"Glioma is one of the most malignant types of cancer and most gliomas remain incurable. One of the hallmarks of glioma is its invasiveness. Furthermore, glioma cells tend to readily detach from the primary tumor and travel through the brain tissue, making complete tumor resection impossible in many cases. To expand the knowledge regarding the invasive behavior of glioma, evaluate drug resistance, and recapitulate the tumor microenvironment, various modeling strategies were proposed in the last decade, including three-dimensional (3D) biomimetic scaffold-free cultures, organ-on-chip microfluidics chips, and 3D bioprinting platforms, which allow for the investigation on patient-specific treatments. The emerging method of 3D bioprinting technology has introduced a time- and cost-efficient approach to create in vitro models that possess the structural and functional characteristics of human organs and tissues by spatially positioning cells and bioink. Here, we review emerging 3D bioprinted models developed for recapitulating the brain environment and glioma tumors, with the purpose of probing glioma cell invasion and gliomagenesis and discuss the potential use of 4D printing and machine learning applications in glioma modelling.","PeriodicalId":74582,"journal":{"name":"Progress in biomedical engineering (Bristol, England)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44184333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-26DOI: 10.1088/2516-1091/ac73c9
Tanziela Tanziela, Xiawei Dong, Jing Ye, Zengchao Guo, Hui Jiang, Zuhong Lu, Xiaohui Liu, Xuemei Wang
Exosomes have emerged as natural nanocarriers and are advantageous in the field of nanomedicine due to their lipid bilayer membrane comprising many proteins, nucleic acids and cell debris. Exosomes are secreted from all types of living cells and play a role in cancer diagnosis and therapy because of their biological properties, such as intercellular communication, modulation of immune responses, biocompatibility and target specificity. Many studies have shown that exosomes can be engineered or modified with different therapeutic substances, including nucleic acids, proteins, drugs and other nanomaterials, to improve their specificity, efficiency and safety in nanomedicine. In this review, we summarize the methodologies of exosome biogenesis, purification, the possible mechanisms of cellular uptake and the important role of exosomes in cancer diagnosis, followed by the role of engineered exosomes in cancer therapy. Also, future trends and challenges are discussed. We strongly suggest that a clear articulation of the fundamental principles for the creation of exosome-based theranostic platforms will help reveal the unique powers of exosomes in early cancer diagnosis and therapeutics, including chemotherapy, gene therapy, immunotherapy and phototherapy.
{"title":"Advances in engineered exosomes towards cancer diagnosis and therapeutics","authors":"Tanziela Tanziela, Xiawei Dong, Jing Ye, Zengchao Guo, Hui Jiang, Zuhong Lu, Xiaohui Liu, Xuemei Wang","doi":"10.1088/2516-1091/ac73c9","DOIUrl":"https://doi.org/10.1088/2516-1091/ac73c9","url":null,"abstract":"Exosomes have emerged as natural nanocarriers and are advantageous in the field of nanomedicine due to their lipid bilayer membrane comprising many proteins, nucleic acids and cell debris. Exosomes are secreted from all types of living cells and play a role in cancer diagnosis and therapy because of their biological properties, such as intercellular communication, modulation of immune responses, biocompatibility and target specificity. Many studies have shown that exosomes can be engineered or modified with different therapeutic substances, including nucleic acids, proteins, drugs and other nanomaterials, to improve their specificity, efficiency and safety in nanomedicine. In this review, we summarize the methodologies of exosome biogenesis, purification, the possible mechanisms of cellular uptake and the important role of exosomes in cancer diagnosis, followed by the role of engineered exosomes in cancer therapy. Also, future trends and challenges are discussed. We strongly suggest that a clear articulation of the fundamental principles for the creation of exosome-based theranostic platforms will help reveal the unique powers of exosomes in early cancer diagnosis and therapeutics, including chemotherapy, gene therapy, immunotherapy and phototherapy.","PeriodicalId":74582,"journal":{"name":"Progress in biomedical engineering (Bristol, England)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43818913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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