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Enhanced spin-polarization and detection limit in a spin-based optoelectrochemical DNA hybridization sensor induced by circularly polarized light† 圆偏振光诱导自旋光电化学 DNA 杂交传感器增强自旋偏振和探测极限
IF 3.5 Q2 CHEMISTRY, ANALYTICAL Pub Date : 2024-05-28 DOI: 10.1039/D4SD00109E
Mayank Tiwari and Debabrata Mishra

We investigate the influence of the correlation between different types of polarized light (linear and circular) and spin-polarization |Ps| (in %) on the effectiveness of a spin-based quantum dot-modified DNA device for a DNA hybridization sensor. The device exhibits a significant two-fold increase in |Ps| (in %) when exposed to circularly polarized (C.P.) light, in comparison to the state of no illumination. This improvement in |Ps| results in a significant ten-fold enhancement in the limit of detection (L.O.D.) of the electrode under C.P. light illumination, surpassing the conditions without illumination and even achieving a two-fold increase compared to linearly polarized (L.P.) light illumination. These results emphasize the crucial significance of polarized light in maximizing the efficiency of spin-based DNA hybridization sensors. The significant enhancements in the performance observed under C.P. light illumination demonstrate the potential use of our device to function as a highly sensitive and efficient tool in spin-based bio-sensing applications.

我们研究了不同类型的偏振光(线性偏振光和圆偏振光)与自旋偏振 |Ps|(%)之间的相关性对基于自旋量子点修饰的 DNA 器件在 DNA 杂交传感器中的有效性的影响。与无光照状态相比,该装置在圆偏振光(C.P.)照射下的|Ps|(单位:%)显著增加了两倍。在 C.P. 光照射下,|Ps|的提高使电极的检测限(L.O.D.)显著提高了十倍,超过了无照射条件,甚至比线性偏振光(L.P.)照射下提高了两倍。这些结果表明,偏振光对于最大限度地提高自旋 DNA 杂交传感器的效率至关重要。在 C.P.光照射下观察到的性能大幅提高表明,我们的设备有可能在基于自旋的生物传感应用中发挥高灵敏度和高效工具的作用。
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引用次数: 0
A triphenylamine scaffold for fluorogenic sensing of noxious cyanide via the ICT mechanism and its bioimaging application† 通过 ICT 机制实现有毒氰化物荧光感应的三苯胺支架及其生物成像应用
IF 3.5 Q2 CHEMISTRY, ANALYTICAL Pub Date : 2024-05-23 DOI: 10.1039/D4SD00018H
Amitav Biswas, Rimi Mukherjee, Atanu Maji, Rahul Naskar, Krishnendu Aich, Nabendu Murmu and Tapan K. Mondal

A novel triphenylamine benzimidazole based fluorogenic chemosensor named (2E,2′E)-3,3′-((phenylazanediyl)bis(4,1-phenylene))bis(2-(1H-benzo[d]imidazol-2-yl)acrylonitrile) (PBIA) has been successfully generated and characterized by varoius spectroscopic techniques. Among various screened anions, only cyanide (CN) showed a distinct fluorogenic property towards PBIA. Hence, the optical properties of PBIA were investigated in the presence of cyanide (CN) by means of UV-vis spectrophotometry and fluorescence spectroscopy in DMSO, where we observed that, upon treatment with CN to the probe solution, the orange fluorescence of the ligand showed a blue shift and the orange fluorescence changed to greenish-yellow under an UV lamp. The hypsochromic shift in fluorescence maxima upon the addition of cyanide was attributed to nucleophilic addition of cyanide to PBIA inhibiting the electron flow within the molecule and disrupting the ICT process. The interaction behind the sensing of cyanide was investigated by 1H-NMR titration, a mass spectroscopic study and DFT calculations, which supported the mechanism. The limit of detection (LOD) was calculated and found to be in the order of 10−8 (M). PBIA showed an immediate response in the spectral pattern (<20 s) towards its target cyanide ion, and the effectiveness of the chemosensor was also examined in the presence of competing anions. Furthermore, the practical efficacy of the PBIA was established by a dipstick experiment along with cyanide detection in various natural water resources. Human breast cancer cells MDA-MB 231 were made susceptible to CN sensing in a biological system.

一种基于三苯胺苯并咪唑的新型荧光化学传感器--(2E,2'E)-3,3'-((苯基氮二基)双(4,1-亚苯基))双(2-(1H-苯并[d]咪唑-2-基)丙烯腈)(PBIA)已被成功制备出来,并通过各种光谱技术对其进行了表征。在筛选出的各种阴离子中,只有氰化物(CN-)对 PBIA 具有明显的致氟特性。因此,我们通过紫外-可见分光光度法和二甲基亚砜中的荧光光谱法研究了 PBIA 在氰化物(CN-)存在下的光学特性,观察到在探针溶液中加入 CN- 后,配体的橙色荧光发生蓝移,在紫外灯下橙色荧光变为灰黄色。在加入氰化物后,荧光最大值发生了同色转移,这是因为氰化物与 PBIA 的亲核加成抑制了分子内的电子流,破坏了 ICT 过程。通过 1H-NMR 滴定、质谱研究和 DFT 计算研究了氰化物传感背后的相互作用,结果支持了这一机制。经计算发现,其检测限(LOD)约为 10-8(M)。PBIA 在光谱模式中对目标氰离子显示出即时响应(20 秒),并在存在竞争阴离子的情况下检验了化学传感器的有效性。此外,探针 PBIA 的实际功效还通过浸渍棒实验和各种天然水资源中的氰化物检测得到了证实。人类乳腺癌细胞 MDA-MB 231 使其在生物系统中易受氯化萘感应。
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引用次数: 0
Antibody fluorescein-doped silica nanobioconjugates for the ultrasensitive detection of prostate-specific antigen† 用于超灵敏检测前列腺特异性抗原的抗体荧光素掺杂二氧化硅纳米生物共轭物
IF 3.5 Q2 CHEMISTRY, ANALYTICAL Pub Date : 2024-05-17 DOI: 10.1039/D4SD00119B
Tumelo Msutu, Omotayo Adeniyi and Philani Mashazi

Herein, we report the bioconjugation of anti-prostate-specific antigen polyclonal antibodies (pAb) onto the fluorescein-doped silica nanoparticles to detect prostate-specific antigen (PSA). Fluorescein-isothiocyanate (FITC), a fluorescent dye, was reacted with (3-aminopropyl)triethoxysilane (APTES) to form the FITC-APTES organosilane precursor. FITC-APTES was mixed with tetraethoxysilane (TEOS) to form fluorescent silica nanoparticles (FITC@SiO2NPs) containing 3% and 6% of dye loading. The silica nanoparticles prevented the dye from leaching and promoted the fluorescent signal amplification for the detection of PSA. Phenylboronic acid (PBA) was coated onto the fluorescent silica nanoparticles for the oriented antibody immobilization via the boronate ester to form FITC@SiO2-PBA-pAb. The fluorescent silica nanobioconjugates exhibited an emission peak at 518 nm, which was stable over time. A fluorescence sandwich-type immunoassay was used for the detection of PSA using FITC@SiO2-PBA-pAb. Alkaline hydrolysis of the sensing nanobioconjugates afforded enhanced sensitivity by releasing FITC molecules. In buffer samples, the fluorescent immunosensor exhibited a linear correlation range from 2.0 pg mL−1 to 50 ng mL−1. The linear range was from 2.0 pg mL−1 to 100 ng mL−1 in newborn calf serum (representing real samples). The limit of detection (LOD) was 8.25 fg mL−1 with a limit of quantification (LOQ) of 27.2 fg mL−1 in PBS (pH 7.4) after NaOH dissolution. A fluorescence immunosensor was used to detect PSA in spiked newborn calf serum with NaOH dissolution. It exhibited an LOD value of 33.0 fg mL−1 and LOQ value of 0.109 pg mL−1. The developed fluorescence immunosensor showed high selectivity and specificity for PSA. The detection of prostate-specific antigen in newborn calf serum samples exhibited no matrix interferences.

在此,我们报告了将抗前列腺特异性抗原多克隆抗体(pAb)生物共轭到掺杂荧光素的二氧化硅纳米颗粒上以检测前列腺特异性抗原(PSA)的方法。使用的荧光染料是荧光素-异硫氰酸酯(FITC),它与(3-氨基丙基)三乙氧基硅烷(APTES)反应生成 FITC-APTES 有机硅烷前体。将 FITC-APTES 与四乙氧基硅烷(TEOS)混合,形成含有 3% 和 6% 染料的荧光纳米二氧化硅颗粒(FITC@SiO2NPs)。二氧化硅纳米粒子可防止染料浸出,并促进荧光信号放大,用于检测 PSA。将苯硼酸(PBA)涂布在荧光纳米二氧化硅颗粒上,通过硼酸酯将抗体定向固定,形成 FITC@SiO2-PBA-pAb。这种荧光纳米二氧化硅生物共轭物在 518 纳米波长处显示出强烈的激发依赖性发射,并且长期稳定。利用 FITC@SiO2-PBA-pAb 的荧光夹心型免疫测定法检测 PSA。传感纳米生物共轭物经碱性水解后释放出 FITC 分子,从而提高了灵敏度。在缓冲液样品中,荧光免疫传感器的线性相关范围为 2.0 pg.mL-1 至 50 ng.mL-1。在新生小牛血清(代表真实样本)中,线性范围为 2.0 pg.mL-1 至 100 ng.mL-1。在 NaOH 溶解后的 PBS 缓冲液(pH 7.4)中,检测限(LOD)为 8.25 fg.mL-1,定量限(LOQ)为 27.2 fg.mL-1。研究了一种荧光免疫传感器,用于检测添加 NaOH 溶解物的新生小牛血清中的 PSA。LOD为33.0 fg.mL-1,LOQ为0.109 pg.mL-1。所开发的荧光免疫传感器对前列腺特异性抗原具有很高的选择性和特异性。在检测新生小牛血清样品中的前列腺特异性抗原时,未发现基质干扰。
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引用次数: 0
Red and NIR active dipod–SDS self-assemblies for “turn on” quantification of spermine in serum, urine and food: smart-phone assisted on-site determination of spermine in amine-rich foods† 用于 "开启 "血清、尿液和食品中精胺定量的红色和近红外活性二极体-SDS 自组装:智能手机辅助现场测定富含胺的食品中的精胺
Pub Date : 2024-05-15 DOI: 10.1039/D3SD00300K
Nancy Singla, Sukhvinder Dhiman, Manzoor Ahmad, Satwinderjeet Kaur, Prabhpreet Singh and Subodh Kumar

Spermine is a vital biomarker for clinical diagnosis of cancer and estimating food spoilage. Here, supramolecular assemblies of two donor–π–acceptor dipods R-SPM (λem 640 nm) and NIR-SPM (λem 720 nm) with SDS have been discovered for the detection of spermine and spermidine under physiological conditions at nanomolar levels. The addition of SDS to R-SPM and NIR-SPM results in the formation of self-assemblies (DLS, zeta-potential and UV-vis studies) with no significant change in their fluorescence but further addition of spermine/spermidine to the R-SPMSDS and NIR-SPMSDS assemblies results in a 30–80 fold increase in fluorescence intensity, respectively at 640 nm and 720 nm. The LOD for spermine and spermidine detection is 22 nM (4.4 ppb) and 67 nM (9.7 ppb). The ensembles show nominal interference from other biogenic amines, amino acids, metal ions, and anions. Both R-SPMSDS and NIR-SPMSDS ensembles can be stored in the dark for >3 months without affecting their performance. The potential of these ensembles for real world applications like analysis of spermine in urine, human serum and food spoilage in the case of cheese, mushrooms, chicken and mutton has been demonstrated. The smartphone relied RGB analysis facilitates the on-site determination of spermine in food samples.

精胺是一种重要的生物标志物,可用于癌症的临床诊断和食品腐败的评估。在这里,我们发现了两种供体-π-受体二极体 R-SPM(λem 640 nm)和 NIR-SPM(λem 720 nm)与 SDS 的超分子组装体,可在生理条件下检测纳摩尔水平的精胺和亚精胺。在 R-SPM 和 NIR-SPM 中加入 SDS 会形成自组装(DLS、zeta 电位和 UV-vis 研究),其荧光没有显著变化,但在 R-SPM∩SDS 和 NIR-SPM∩SDS 组装中进一步加入精胺/亚精胺会使 640 纳米和 720 纳米波长处的荧光强度分别增加 30-80 倍。精胺和亚精胺的检测限分别为 22 nM(4.4 ppb)和 67 nM(9.7 ppb)。组合物显示,其他生物胺、氨基酸、金属离子和阴离子的干扰很小。R-SPM∩SDS 和 NIR-SPM∩SDS 组合可在黑暗中保存 3 个月而不影响其性能。这些组件在实际应用中的潜力已得到证实,如分析尿液中的精胺、人体血清以及奶酪、蘑菇、鸡肉和羊肉中的食品腐败。依靠智能手机进行的 RGB 分析有助于现场测定食品样本中的精胺。
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引用次数: 0
Effects of storage conditions on the performance of an electrochemical aptamer-based sensor† 储存条件对基于电化学贴体的传感器性能的影响
Pub Date : 2024-05-14 DOI: 10.1039/D4SD00066H
Julia Chung, Adriana Billante, Charlotte Flatebo, Kaylyn K. Leung, Julian Gerson, Nicole Emmons, Tod E. Kippin, Lior Sepunaru and Kevin W. Plaxco

The electrochemical aptamer-based (EAB) sensor platform is the only molecular monitoring approach yet reported that is (1) real time and effectively continuous, (2) selective enough to deploy in situ in the living body, and (3) independent of the chemical or enzymatic reactivity of its target, rendering it adaptable to a wide range of analytes. These attributes suggest the EAB platform will prove to be an important tool in both biomedical research and clinical practice. To advance this possibility, here we have explored the stability of EAB sensors upon storage, using retention of the target recognizing aptamer, the sensor's signal gain, and the affinity of the aptamer as our performance metrics. Doing so we find that low-temperature (−20 °C) storage is sufficient to preserve sensor functionality for at least six months without the need for exogenous preservatives.

基于电化学适配体(EAB)的传感器平台是目前报道的唯一一种分子监测方法,它具有以下特点:(1)实时性和有效的连续性;(2)选择性强,可在活体内原位部署;(3)不受其目标的化学或酶反应性影响,因此可适用于多种分析物。这些特性表明,EAB 平台将成为生物医学研究和临床实践的重要工具。为了推进这种可能性,我们在这里探索了 EAB 传感器在存储过程中的稳定性,并将目标识别适配体的保留、传感器的信号增益和适配体的亲和力作为我们的性能指标。我们发现,低温(-20 °C)储存足以在不使用外源防腐剂的情况下保持传感器功能至少六个月。
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引用次数: 0
Correction: Highly sensitive solid-state nanopore aptasensor based on target-induced strand displacement for okadaic acid detection from shellfish samples 更正:基于目标诱导链位移的高灵敏度固态纳米孔准传感器用于检测贝类样品中的冈田酸
Pub Date : 2024-05-14 DOI: 10.1039/D4SD90015D
Mohamed Amin Elaguech, Yajie Yin, Yunjiao Wang, Bing Shao, Chaker Tlili and Deqiang Wang

Correction for ‘Highly sensitive solid-state nanopore aptasensor based on target-induced strand displacement for okadaic acid detection from shellfish samples’ by Mohamed Amin Elaguech et al., Sens. Diagn., 2023, 2, 1612–1622, https://doi.org/10.1039/D3SD00199G.

对 Mohamed Amin Elaguech 等人撰写的 "基于目标诱导链位移的高灵敏度固态纳米孔准传感器用于检测贝类样品中的冈田酸 "的更正,Sens.Diagn., 2023, 2, 1612-1622, https://doi.org/10.1039/D3SD00199G.
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引用次数: 0
Aptamer-functionalized nanopipettes: a promising approach for viral fragment detection via ion current rectification† 色素功能化纳米移液管:通过离子电流整流检测病毒片段的有效方法
Pub Date : 2024-05-10 DOI: 10.1039/D4SD00097H
Shekemi Denuga, Dominik Duleba, Pallavi Dutta, Guerrino Macori, Damion K. Corrigan, Séamus Fanning and Robert P. Johnson

In this report, ion current rectification, an electrochemical phenomenon observed in asymmetric nanopipettes, is used for the label-free detection of SARS-CoV-2 viral fragments in nasopharyngeal samples. Quartz nanopipettes are functionalized with aptamers targeting the spike protein S1 domain, wherein changes to the surface charge magnitude, distribution, and ion transport behavior modulate the current–voltage response upon binding. The aptamer-modified nanopipette provides a selective and sensitive method for detecting SARS-CoV-2, with a limit of detection in the laboratory of 0.05 pg mL−1. The effectiveness of this low-cost platform was demonstrated by sensing SARS-CoV-2 in nasopharyngeal samples, successfully discriminating between positive and negative cases with minimal template preparation, highlighting the platform's potential as a versatile sensing strategy for infectious disease detection in clinical diagnosis.

本报告利用在不对称纳米吸头中观察到的电化学现象--离子电流整流,对鼻咽样本中的 SARS-CoV-2 病毒片段进行了无标记检测。石英纳米吸管用针对尖峰蛋白 S1 结构域的适配体进行了功能化处理,表面电荷量、分布和离子传输行为的变化调节了结合后的电流-电压响应。经aptamer修饰的纳米吸管为检测SARS-CoV-2提供了一种选择性强、灵敏度高的方法,在实验室中的检测限为0.05 pg/mL。通过检测鼻咽样本中的 SARS-CoV-2 证明了这一低成本平台的有效性,只需制备极少的模板就能成功区分阳性和阴性病例,凸显了该平台作为临床诊断中传染病检测的多功能传感策略的潜力。
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引用次数: 0
Application of breathomics in pediatric asthma: a review 呼吸组学在小儿哮喘中的应用:综述
Pub Date : 2024-05-09 DOI: 10.1039/D3SD00286A
Lei Chi, Xiaoli Wang, Yuxia Shan, Chonghui Zhu, Ling Leng, Rong Chen, Qing Xie, Zhenze Cui and Minghui Yang

Breathomics involves the use of non-invasive methods for diagnosing asthma by analyzing exhaled breath. While significant progress has been made in applying this approach to adult asthma, extending its application to pediatric asthma is crucial due to the increasing concern in this population. This review delineates five potential clinical applications: asthma diagnosis, differential diagnosis of asthma, assessment of asthma control levels, prediction of asthma exacerbation, and asthma phenotyping. Additionally, it highlights the moderate to reasonable predictive accuracy of exhaled breath volatile organic compounds (VOCs) breathomics in childhood asthma. However, it acknowledges that this field is still in its nascent stage of development, with particularly limited data available for Asian populations. Moreover, the identification of VOC biomarkers in pediatric asthma patients remains inconclusive, with varying reports. Therefore, large-scale data collection and standardization are imperative. Refinement and methodological improvements are necessary before integrating breathomics into clinical practice. This article provides clear directions for future research to optimize the clinical applicability of breathomics in evaluating asthma in children.

呼吸组学是一种通过分析呼出的气体来诊断哮喘的非侵入性方法。虽然在将这种方法应用于成人哮喘方面已经取得了重大进展,但将其应用扩展到小儿哮喘至关重要,因为小儿哮喘日益受到关注。本综述阐述了五种潜在的临床应用:哮喘诊断、哮喘鉴别诊断、哮喘控制水平评估、哮喘恶化预测和哮喘表型。此外,该研究还强调了呼出气体挥发性有机化合物(VOCs)呼吸组学对儿童哮喘的预测准确性为中等至合理水平。不过,该研究承认,这一领域仍处于发展的初级阶段,尤其是针对亚洲人群的数据非常有限。此外,儿科哮喘患者体内挥发性有机化合物生物标志物的鉴定仍无定论,报告也不尽相同。因此,大规模的数据收集和标准化工作势在必行。在将呼吸组学应用于临床实践之前,有必要对方法进行完善和改进。本文为未来的研究提供了明确的方向,以优化呼吸组学在儿童哮喘评估中的临床应用。
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引用次数: 0
Advances in electrochemical sensors for real-time glucose monitoring 用于实时葡萄糖监测的电化学传感器的进展
Pub Date : 2024-05-08 DOI: 10.1039/D4SD00086B
Md. Harun-Or-Rashid, Most. Nazmin Aktar, Veronica Preda and Noushin Nasiri

Technological advancements are revolutionizing diabetic care worldwide, particularly in the realm of glucose monitoring. Traditionally invasive and cumbersome, glucose monitoring is shifting towards less invasive methods, enhancing patient quality of life and reducing risks associated with hypo- and hyperglycemia. Wearable biosensors, focusing on sweat and interstitial fluid, offer novel avenues for early disease detection and personalized point-of-care testing. This review paper provides a comprehensive overview of recent strides in wearable sweat sensors, including historical perspectives, electrochemical sensing mechanisms, material advancements, and the role of nanomaterials in enhancing sensor performance. By examining the evolution of glucose monitoring devices and highlighting commercially available devices, the review underscores the wide-ranging utility of electrochemical sensors in glucose monitoring. Enzymatic and non-enzymatic sensing mechanisms, potentiometric, amperometric/voltammetric sensors, ion-selective electrodes, and biosensors are discussed in detail, alongside various materials employed to optimize sensor performance. The burgeoning interest in nanomaterial-enabled sensor platforms signifies a promising future for sweat-based glucose monitoring, with potential implications for personalized healthcare and disease management.

技术进步正在彻底改变全世界的糖尿病护理,尤其是在血糖监测领域。传统的血糖监测既有创又繁琐,现在正向微创方法转变,从而提高了患者的生活质量,降低了与低血糖和高血糖相关的风险。以汗液和组织间液为重点的可穿戴生物传感器为早期疾病检测和个性化护理点检测提供了新的途径。本综述全面概述了可穿戴汗液传感器的最新进展,包括历史观点、电化学传感机制、材料进步以及纳米材料在提高传感器性能方面的作用。通过研究葡萄糖监测设备的演变过程和重点介绍市售设备,该综述强调了电化学传感器在葡萄糖监测中的广泛用途。文章详细讨论了酶和非酶传感机制、电位传感器、安培/伏安传感器、离子选择性电极和生物传感器,以及用于优化传感器性能的各种材料。人们对纳米材料传感器平台的兴趣日益浓厚,这标志着基于汗液的葡萄糖监测前景广阔,对个性化医疗保健和疾病管理具有潜在影响。
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引用次数: 0
Contactless vital sign monitoring systems: a comprehensive survey of remote health sensing for heart rate and respiration in internet of things and sleep applications 非接触式生命体征监测系统:全面调查
IF 3.5 Q2 CHEMISTRY, ANALYTICAL Pub Date : 2024-05-08 DOI: 10.1039/D4SD00073K
Muhammad Salman Raheel, Faisel Tubbal, Raad Raad, Philip Ogunbona, James Coyte, Christopher Patterson, Dana Perlman, Saeid Iranmanesh, Nidhal Odeh and Javad Foroughi

With the coronavirus pandemic, companies and governments around the world have been investing millions of dollars in the development of contactless sensor technologies that minimize the need for physical interactions between patients and healthcare providers. This has led to rapid progress in healthcare research on innovative contactless technologies, particularly for infants and elderly individuals with chronic diseases that require continuous, real-time monitoring and control. The combination of sensing technology and wireless communication has emerged as a promising research area, as patients often find it unpleasant or anxiety-provoking to wear sensor devices, and physical contact can exacerbate the spread of contagious diseases. To address these issues, research has focused on sensor-less or contactless technology to send and analyse wireless signals to remotely monitor and measure vital signs without requiring physical contact or sensor devices. Herein, we have provided a comprehensive survey and study of non-invasive/contactless vital sign monitoring systems, particularly the heart rate and the respiration rate monitoring systems to achieve accurate and reliable measurements. We have found that there is a lack of a comprehensive comparison and analysis over existing contactless vital sign monitoring systems. Therefore, we first present and classify the existing non-invasive monitoring designs based on their approaches and techniques, and then compare them based on the performance and accuracy.

随着冠状病毒的大流行,世界各地的公司和政府纷纷投资数百万美元开发非接触式传感器技术,以最大限度地减少病人和医疗服务提供者之间的物理交互。这促使创新型非接触式技术的医疗保健研究取得了快速进展,尤其是针对需要持续、实时监测和控制的婴儿和患有慢性疾病的老年人。传感技术与无线通信的结合已成为一个前景广阔的研究领域,因为患者通常会觉得佩戴传感设备令人不快或焦虑,而且身体接触会加剧传染病的传播。为了解决这些问题,研究重点放在了无传感器或非接触式技术上,以发送和分析无线信号,从而在不需要身体接触或传感器设备的情况下远程监控和测量生命体征。在此,我们对无创/非接触式生命体征监测系统进行了全面调查和研究,特别是心率和呼吸率监测系统,以实现准确可靠的测量。我们发现,对现有的非接触式生命体征监测系统缺乏全面的比较和分析。因此,我们首先根据方法和技术对现有的无创监测设计进行了介绍和分类,然后根据性能和准确性对它们的性能进行了比较。
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引用次数: 0
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