This study aimed to investigate the performance and gene expression of calbindin-D28K (CALB), interleukin-10 (IL-10), and interleukin-1β (IL-1β) in 21-day-old broilers from breeders of different ages, supplemented or not with 1,25-dihydroxycholecalciferol glycoside (1,25(OH)2D3-glycoside). A total of 8000 AP95 (Aviagen) breeders, at 21 weeks of age, were evenly distributed between two houses. In one house, the birds received a corn and soybean meal-based diet supplemented with 100 mg/kg of 1,25(OH)2D3-glycoside (providing 1 µg of active 1,25(OH)2D3 per kg of feed), while in the other, the same diet was provided without the supplementation. When the breeders reached 30, 46, and 62 weeks of age, their eggs were incubated. After hatching, three trials were conducted, each with 384 one-day-old male broilers originating from breeders aged 30 (Trial I) 46 (Trial II), and 62 weeks (Trial III), respectively. All trials consisted of two treatments, based on whether the breeders’ diets were supplemented with 1,25(OH)2D3-glycoside or not. In every trial, broilers were fed a diet free of 1,25(OH)2D3-glycoside until 21 days of age, meeting the nutritional requirements for this phase. Breeders aged 46 and 62 weeks, supplemented with 1,25(OH)2D3-glycoside, produced broilers with an initial average weight superior to that of broilers from breeders without supplementation. Comparing the trials, broilers from breeders aged 46 and 62 weeks showed higher feed intake, greater weight gain, and better feed conversion at 21 days of age. Broilers from breeders aged 62 weeks, supplemented with 1,25(OH)2D3-glycoside, showed higher gene expression of CALB, IL-10, and IL-1β at 21 days of age compared to broilers from breeders that did not receive 1,25(OH)2D3-glycoside. Comparing the trials, broilers from breeders aged 46 and 62 weeks supplemented with 1,25(OH)₂D₃-glycoside had the highest gene expression of IL-10 and IL-1β. It is concluded that breeders aged 46 and 62 weeks, when supplemented with 1,25(OH)2D3-glycoside, produce broilers with higher weight at hatch, as well as enhanced performance and increased gene expression of CALB, IL-10, and IL-1β at 21 days of age.
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