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Short communication: Performance and gene expression in broilers from breeders of different ages, supplemented or not with 1,25(OH)2D3-glycoside 短沟通:不同日龄种鸡在添加或不添加1,25(OH) 2d3 -糖苷后的生产性能和基因表达
IF 2.7 2区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2025-11-15 DOI: 10.1016/j.anifeedsci.2025.116571
E.A. Iachinski , T.S. Andrade , N. Rohloff Junior , B.A. Bebber , R.A. Bombardelli , J.G. Vargas Junior , A.A. Calderano , C. Eyng , R.V. Nunes
This study aimed to investigate the performance and gene expression of calbindin-D28K (CALB), interleukin-10 (IL-10), and interleukin-1β (IL-1β) in 21-day-old broilers from breeders of different ages, supplemented or not with 1,25-dihydroxycholecalciferol glycoside (1,25(OH)2D3-glycoside). A total of 8000 AP95 (Aviagen) breeders, at 21 weeks of age, were evenly distributed between two houses. In one house, the birds received a corn and soybean meal-based diet supplemented with 100 mg/kg of 1,25(OH)2D3-glycoside (providing 1 µg of active 1,25(OH)2D3 per kg of feed), while in the other, the same diet was provided without the supplementation. When the breeders reached 30, 46, and 62 weeks of age, their eggs were incubated. After hatching, three trials were conducted, each with 384 one-day-old male broilers originating from breeders aged 30 (Trial I) 46 (Trial II), and 62 weeks (Trial III), respectively. All trials consisted of two treatments, based on whether the breeders’ diets were supplemented with 1,25(OH)2D3-glycoside or not. In every trial, broilers were fed a diet free of 1,25(OH)2D3-glycoside until 21 days of age, meeting the nutritional requirements for this phase. Breeders aged 46 and 62 weeks, supplemented with 1,25(OH)2D3-glycoside, produced broilers with an initial average weight superior to that of broilers from breeders without supplementation. Comparing the trials, broilers from breeders aged 46 and 62 weeks showed higher feed intake, greater weight gain, and better feed conversion at 21 days of age. Broilers from breeders aged 62 weeks, supplemented with 1,25(OH)2D3-glycoside, showed higher gene expression of CALB, IL-10, and IL-1β at 21 days of age compared to broilers from breeders that did not receive 1,25(OH)2D3-glycoside. Comparing the trials, broilers from breeders aged 46 and 62 weeks supplemented with 1,25(OH)₂D₃-glycoside had the highest gene expression of IL-10 and IL-1β. It is concluded that breeders aged 46 and 62 weeks, when supplemented with 1,25(OH)2D3-glycoside, produce broilers with higher weight at hatch, as well as enhanced performance and increased gene expression of CALB, IL-10, and IL-1β at 21 days of age.
本试验旨在研究21日龄不同日龄种鸡在饲粮中添加或不添加1,25-二羟基胆钙化醇糖苷(1,25(OH) 2d3 -糖苷)时,钙结合素- d28k (CALB)、白细胞介素-10 (IL-10)和白细胞介素-1β (IL-1β)的生产性能和基因表达。选取21周龄的AP95种鸡8000只,均匀分布在2个鸡舍中。在一组试验中,饲粮中添加100 mg/kg的1,25(OH)2D3-糖苷(每千克饲料中含有1 µg的活性1,25(OH)2D3),而在另一组试验中,饲粮中不添加该物质。当繁殖者达到30、46和62周龄时,它们的蛋被孵化。孵化后进行3次试验,每次试验384只1日龄雄性肉鸡,分别来自30岁(试验1)、46岁(试验2)和62周龄(试验3)的种鸡。根据饲粮中是否添加125 (OH) 2d3 -糖苷,所有试验均分为两种处理。在每个试验中,肉鸡在21日龄前均饲喂不含125 (OH) 2d3 -糖苷的饲粮,以满足该阶段的营养需求。46周龄和62周龄的种鸡,在饲粮中添加125 (OH) 2d3 -糖苷,所产肉鸡的初始平均体重优于未添加的种鸡。比较试验结果,46周龄和62周龄种鸡在21日龄时的采食量、增重和饲料转化率均较高。62周龄饲粮中添加1.25 (OH) 2d3 -糖苷的肉仔鸡在21日龄时CALB、IL-10和IL-1β的基因表达高于未添加1.25 (OH) 2d3 -糖苷的肉仔鸡。比较试验结果,饲粮中添加125 (OH)₂D₃-糖苷的46周龄和62周龄肉鸡IL-10和IL-1β基因表达量最高。综上所述,46周龄和62周龄的种鸡在饲粮中添加1,25(OH) 2d3 -糖苷,可提高肉鸡的孵化体重,提高21日龄时肉鸡的生产性能,提高CALB、IL-10和IL-1β的基因表达。
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引用次数: 0
Corrigendum to “Improving lipid utilization and growth through lecithin inclusion in diets for giant grouper (Epinephelus lanceolatus)” [Anim. Feed Sci. Technol. 326 (2025) 116393] “通过在石斑鱼(Epinephelus lanceolatus)的饲料中添加卵磷脂来提高脂质利用和生长”的勘误表[动物]。饲料科学。科技. 326 (2025)116393]
IF 2.7 2区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2025-11-15 DOI: 10.1016/j.anifeedsci.2025.116569
Grace Butler , Caroline Lourdes Candebat , Simon Kumar Das , Leo Nankervis
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引用次数: 0
Mixture extracts of Psidium guajava L. and Azadirachta indica A. Juss - based diets synergistically improve health status through red tilapia (Oreochromis sp.) immune responses, antioxidant activities, and resistance to bacterial pathogens 番石榴石蕊和印楝混合提取物通过红罗非鱼(Oreochromis sp.)的免疫应答、抗氧化活性和对细菌病原体的抗性,协同改善红罗非鱼的健康状况
IF 2.7 2区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2025-11-11 DOI: 10.1016/j.anifeedsci.2025.116570
Truong Quynh Nhu , Bui Thi Bich Hang , Nguyen Thi Mai , Nguyen Do Quynh , Tran Thi Tuyet Hoa , Nguyen Thi Thu Hang , Nguyen Thi Kim Ha , Tran Ngoc Bich , Vu Ngoc Minh Thu , Nguyen Thanh Phuong , Patrick Kestemont
The study investigated the effects of guava and neem extract-based diets, alone or in combination, on the immune responses, antioxidative activities and disease resistance in red tilapia (Oreochromis sp.). Fish were randomly assigned to seven treatments: a control diet (without extract), single guava extract (0.3 %), single neem extract (0.3 %), and guava-neem mixture at 0.1 %:0.05 %, 0.2 %:0.1 %, 0.4 %:0.2 %, and 0.8 %:0.4 % for 6 weeks, followed by being challenged with Streptococcus agalactiae. The results showed that single extract diets did not affect total red blood cells, hematocrit, or white blood cells. In contrast, mixture diets induced strong synergistic effects, significantly enhancing total white blood cells at weeks 3 and 6 compared to the control (p < 0.05). At equivalent total extract concentration (0.3 %), mixture diets synergistically enhanced respiratory burst activity in blood cells in W6 and 3 days post-challenge compared to single and control groups (p < 0.05). Similarly, mixture diets significantly enhanced the humoral immune responses (lysozyme, complement, alkaline phosphatase and peroxidase) in time and dose-dependent manners, while the single extract produced a weaker response compared to control. Antioxidant capacity was markedly improved by the mixture diets (except 0.8 %:0.4 %), with synergistic increases in superoxide dismutase and catalase, accompanied by reduced malondialdehyde levels compared to single and control groups (p < 0.05). Importantly, only the mixture diets significantly reduced cumulative mortality after bacterial infection (p < 0.05), whereas single extracts offered no protection compared to control (p > 0.05). Histological assessment confirmed that the highest mixture (0.8 %:0.4 %) caused no damage to the liver or gut. Dietary supplementation with guava-neem mixtures, particularly at 0.1 %:0.05 %, 0.2 %:0.1 %, and 0.4 %:0.2 %, elicited synergistic enhancements in immune and antioxidant status, providing better protection for red tilapia against S. agalactiae infection.
研究了番石榴和楝树提取物对红罗非鱼免疫反应、抗氧化活性和抗病性的影响。鱼被随机分配到7个治疗:控制饮食(没有提取),单一的番石榴提取(0.3 %),单印楝提取物(0.3 %),和guava-neem混合物0.1 %:0.05 % 0.2 %:0.1 % 0.4 %:0.2 %,和0.8 %:0.4 % 6周,随后与链球菌agalactiae受到挑战。结果表明,单一提取物饮食对总红细胞、红细胞压积或白细胞没有影响。相反,混合饲粮诱导了较强的协同效应,在第3周和第6周时,与对照组相比,总白细胞显著增加(p <; 0.05)。在相同的总提取物浓度(0.3 %)下,与单一组和对照组相比,混合饲粮在攻毒后第6天和第3天协同提高了血细胞的呼吸爆发活性(p <; 0.05)。同样,混合饲料也显著增强了溶菌酶、补体、碱性磷酸酶和过氧化物酶的体液免疫反应,且呈时间和剂量依赖性,而单一提取物的反应较对照组弱。混合饲粮显著提高了抗氧化能力(除0.8 %和0.4 %外),与单一组和对照组相比,超氧化物歧化酶和过氧化氢酶协同增加,丙二醛水平降低(p <; 0.05)。重要的是,只有混合饲料显著降低了细菌感染后的累积死亡率(p <; 0.05),而单一提取物与对照组相比没有保护作用(p <; 0.05)。组织学评估证实,最高混合物(0.8 %:0.4 %)未对肝脏或肠道造成损害。在饲料中添加番石榴-印度树混合物,特别是0.1 %:0.05 %、0.2 %:0.1 %和0.4 %:0.2 %,可以增强红罗非鱼的免疫和抗氧化能力,更好地保护红罗非鱼免受无乳链球菌感染。
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引用次数: 0
Performance and nutrient digestibility of broilers fed barley-based diets supplemented with amylase 添加淀粉酶的大麦基饲粮对肉鸡生产性能和营养物质消化率的影响
IF 2.7 2区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2025-11-11 DOI: 10.1016/j.anifeedsci.2025.116568
Lucas S. Bassi , Khaled Itani , Muhammad Ahmad , Adam Smith , Aaron Cowieson , Sebastian Kaczmarek , Birger Svihus
This study evaluated the effects of exogenous amylase supplementation in barley-based diets for broiler chickens. Two experiments (Exp. 1 and 2) were conducted, in which 11-to-33-d-old broilers were assigned to two dietary treatments (with or without amylase at 81 kg-novo units/kg diet) in a randomized design. Exp. 1 used 352 male broilers (8 replicates of 22 birds) and a low-viscosity normal hulled barley, while Exp. 2 utilized 484 female broilers (11 replicates of 22 birds) and a normal hulled cultivar of high-viscosity. All diets in both trials were supplemented with phytase and a xylanase and β-glucanase complex. In Exp. 1, amylase tended to improve feed conversion ratio (P < 0.1) and increased AID of crude protein (CP) (P < 0.05) at 33 d of age but had no effect on AID of starch. In Exp. 2, no effects of amylase were observed on growth performance, AID of starch and CP, or relative organ weights. Overall findings from both Exp indicate that amylase supplementation can increase CP digestibility of barley-based diets depending on intrinsic viscosity, which may then improve performance. High starch digestibility observed in diets without amylase explains the lack of enzyme effect and suggests the investigated barleys caused no major detrimental effects to nutrient utilization, although the use of high-viscosity barley in Exp. 2 led to lower AID of starch and CP than Exp. 1. The use of a fiber-degrading enzyme likely enhanced starch utilization, leaving limited room for improvements with amylase for broilers at this age.
本研究评价了在大麦基饲粮中添加外源淀粉酶对肉鸡的影响。试验1和试验2采用随机设计,将11 ~ 33日龄肉仔鸡分为添加淀粉酶和不添加淀粉酶两种饲粮处理(添加淀粉酶的水平为81 kg-novo单位/kg)。试验1选用352只雄性肉鸡(8个重复,22只鸡)和一种低黏度普通带壳大麦;试验2选用484只雌性肉鸡(11个重复,22只鸡)和一种高黏度普通带壳大麦品种。两个试验的饲粮均添加植酸酶和木聚糖酶- β-葡聚糖酶复合物。在试验1中,淀粉酶有提高33日龄饲料系数(P <; 0.1)和提高粗蛋白质(CP) AID (P <; 0.05)的趋势,但对淀粉AID没有影响。在试验2中,未观察到淀粉酶对生长性能、淀粉和CP的AID以及相对器官重量的影响。两项试验的总体结果表明,添加淀粉酶可以根据特性粘度提高大麦基饲粮的粗蛋白质消化率,从而提高生产性能。在不添加淀粉酶的饲粮中观察到较高的淀粉消化率,这解释了酶效应的缺乏,表明试验2中使用高粘度大麦导致淀粉和CP的AID低于试验1,但所研究的大麦对营养物质的利用没有造成重大不利影响。纤维降解酶的使用可能会提高淀粉的利用率,在这个年龄的肉仔鸡中使用淀粉酶的改进空间有限。
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引用次数: 0
Effects of heat-killed Lactobacillus plantarum (HK L-137) and propionic acid on growth and health of striped catfish (Pangasianodon hypophthalmus) 热杀植物乳杆菌(hkl -137)和丙酸对斑纹鲶鱼生长和健康的影响
IF 2.7 2区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2025-11-09 DOI: 10.1016/j.anifeedsci.2025.116567
Pham Minh Duc , Tran Thi Thanh Hien , Tran Thi Tuyet Hoa , Tran Ngoc Tuan
Heat-killed Lactobacillus plantarum (HK L-137) is known to benefit striped catfish (Pangasianodon hypophthalmus) health, but its synergistic potential with short-chain fatty acids remains unexplored. This study investigated the individual and combined effects of HK L-137 and propionic acid (PA) on growth performance, feed utilization, immune responses, and stress response in striped catfish. Striped catfish (n = 8400, mean body mass 4.09 ± 0.08 g) were randomly assigned to six feeding treatments with different levels of HK L-137 and PA (0 [control], 1000 mg PA/kg feed [PA.1000], 2 mg HK L-137/kg feed [HK.2], 4 mg HK L-137/kg feed [HK.4], 2 mg HK L-137/kg feed + 100 mg PA/kg feed [HK.2 +PA.100], and 2 mg HK L-137/kg feed + 1000 mg PA/kg feed [HK.2 +PA.1000], for eight weeks (with four replicates for each treatment). Results showed that, compared to the control, HK.2 significantly enhanced growth, improved digestive enzyme activity (amylase, trypsin, and chymotrypsin), and reduced the feed conversion ratio (P < 0.05). PA.1000 increased lymphocyte counts, while both HK.2 and HK.4 elevated cortisol and lipid peroxidase levels (P < 0.05). Furthermore, catalase activity was significantly increased in HK.4 and HK.2 +PA.1000 groups (P < 0.05). Notably, both HK.2 and HK.4 groups exhibited a reduced cortisol response following high-density crowding stress (3000 fish/m3). In conclusion, dietary HK L-137 effectively improved growth, feed efficiency, and digestive function, while also enhancing stress tolerance in striped catfish; however, the synergistic effects with PA were limited. These findings support the application of HK L-137 as a promising functional feed additive for striped catfish aquaculture.
已知热杀灭植物乳杆菌(HK L-137)有益于条纹鲶鱼(Pangasianodon hypophthalmus)的健康,但其与短链脂肪酸的协同作用潜力尚未被探索。本试验研究了HK -137和丙酸(PA)对条纹鲶鱼生长性能、饲料利用、免疫反应和应激反应的单独和联合影响。将n只 = 8400尾,平均体重4.09 ± 0.08 g的条纹鲶鱼随机分为6组,分别添加不同水平的HK -137和PA(0[对照],1000 mg PA/kg饲料[PA])。2 mg HK L-137/kg饲料[HK。[2], 4 mg HK L-137/kg饲料[HK。[4], 2 mg HK L-137/kg饲料+ 100 mg PA/kg饲料[HK.2 +PA]。2 mg HK L-137/kg饲料+ 1000 mg PA/kg饲料[HK.2 +PA]。1000],持续8周(每个处理4个重复)。结果表明,与对照组相比,hk - 2显著促进了生长,提高了消化酶(淀粉酶、胰蛋白酶和凝乳胰蛋白酶)活性,降低了饲料系数(P <; 0.05)。PA.1000增加淋巴细胞计数,而HK.2和HK.4升高皮质醇和脂质过氧化物酶水平(P <; 0.05)。HK.4和HK.2 +PA过氧化氢酶活性显著升高。1000组(P <; 0.05)。值得注意的是,在高密度拥挤胁迫(3000鱼/m3)下,HK.2和HK.4组的皮质醇反应均有所降低。综上所述,饲粮中添加HK L-137可有效提高条纹鲶鱼的生长、饲料效率和消化功能,同时提高应激耐受性;然而,与PA的协同作用有限。这些结果支持HK L-137作为一种有前景的功能性饲料添加剂在条纹鲶鱼养殖中的应用。
{"title":"Effects of heat-killed Lactobacillus plantarum (HK L-137) and propionic acid on growth and health of striped catfish (Pangasianodon hypophthalmus)","authors":"Pham Minh Duc ,&nbsp;Tran Thi Thanh Hien ,&nbsp;Tran Thi Tuyet Hoa ,&nbsp;Tran Ngoc Tuan","doi":"10.1016/j.anifeedsci.2025.116567","DOIUrl":"10.1016/j.anifeedsci.2025.116567","url":null,"abstract":"<div><div>Heat-killed <em>Lactobacillus plantarum</em> (HK L-137) is known to benefit striped catfish (<em>Pangasianodon hypophthalmus</em>) health, but its synergistic potential with short-chain fatty acids remains unexplored. This study investigated the individual and combined effects of HK L-137 and propionic acid (PA) on growth performance, feed utilization, immune responses, and stress response in striped catfish. Striped catfish (n = 8400, mean body mass 4.09 ± 0.08 g) were randomly assigned to six feeding treatments with different levels of HK L-137 and PA (0 [control], 1000 mg PA/kg feed [PA.1000], 2 mg HK L-137/kg feed [HK.2], 4 mg HK L-137/kg feed [HK.4], 2 mg HK L-137/kg feed + 100 mg PA/kg feed [HK.2 +PA.100], and 2 mg HK L-137/kg feed + 1000 mg PA/kg feed [HK.2 +PA.1000], for eight weeks (with four replicates for each treatment). Results showed that, compared to the control, HK.2 significantly enhanced growth, improved digestive enzyme activity (amylase, trypsin, and chymotrypsin), and reduced the feed conversion ratio (<em>P &lt; 0.05</em>). PA.1000 increased lymphocyte counts, while both HK.2 and HK.4 elevated cortisol and lipid peroxidase levels (<em>P &lt; 0.05</em>). Furthermore, catalase activity was significantly increased in HK.4 and HK.2 +PA.1000 groups (<em>P &lt; 0.05</em>). Notably, both HK.2 and HK.4 groups exhibited a reduced cortisol response following high-density crowding stress (3000 fish/m<sup>3</sup>). In conclusion, dietary HK L-137 effectively improved growth, feed efficiency, and digestive function, while also enhancing stress tolerance in striped catfish; however, the synergistic effects with PA were limited. These findings support the application of HK L-137 as a promising functional feed additive for striped catfish aquaculture.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"330 ","pages":"Article 116567"},"PeriodicalIF":2.7,"publicationDate":"2025-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145517356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluating variables affecting Pellet Durability Index (PDI) in pelleted corn-soy-based feeds for swine and poultry: A meta-analysis 猪和家禽用玉米-大豆颗粒饲料中影响颗粒耐久性指数(PDI)的变量评估:一项荟萃分析
IF 2.7 2区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2025-11-08 DOI: 10.1016/j.anifeedsci.2025.116566
Jihao You , Kendra Hall , Jessica Civiero , Mark C. Malpass , Dan Tulpan , Jennifer L. Ellis
Pelleting is one of the most widely used feed processing methods in poultry and swine production systems, as it enhances feed handling, improves animal production efficiency, and reduces feed waste. However, poor pellet quality remains a significant challenge for swine and poultry feed manufacturing, limiting economic returns at both the mill and farm levels. Predicting and optimizing pellet quality can be challenging due to the numerous factors in the feed manufacturing process, including diet formulation, production parameters and environmental conditions. A meta-analysis of published studies was conducted to investigate and quantify the impact of formulation and manufacturing factors on pellet quality and develop predictive equations for Pellet Durability Index (PDI), which represents an in-mill metric for pellet quality. Following a systematic review of the literature, a dataset comprising 16 variables and 280 treatment mean observations was extracted from 29 published studies. Models were developed while treating the study as a random effect. Eight factors were selected for model development, including feed composition factors comprising ingredient inclusions and nutrient content as well as key manufacturing parameters. All models were assessed using a 3-fold cross-validation approach, by placing entire studies into folds. The results showed that the bivariable model using Lipid (g/kg) and Protein (g/kg), resulted in the best PDI prediction performance, with a Concordance Correlation Coefficient of 0.57 ± 0.076, a bias correction factor of 0.91 ± 0.092, and a Pearson Correlation Coefficient of 0.63 ± 0.137. This meta-analysis provides not only a pipeline for selecting models based on a set of statistical criteria, but also produced simple models that can be utilized in feed manufacturing to estimate PDI when data availability is limited.
颗粒化是家禽和猪生产系统中最广泛使用的饲料加工方法之一,因为它提高了饲料处理,提高了动物生产效率,减少了饲料浪费。然而,颗粒质量差仍然是猪和家禽饲料制造业面临的一个重大挑战,限制了工厂和农场层面的经济回报。由于饲料生产过程中的许多因素,包括饲料配方、生产参数和环境条件,预测和优化颗粒质量可能具有挑战性。对已发表的研究进行了荟萃分析,以调查和量化配方和制造因素对颗粒质量的影响,并开发颗粒耐久性指数(PDI)的预测方程,PDI代表颗粒质量的磨内度量。在对文献进行系统回顾后,从29项已发表的研究中提取了包含16个变量和280个治疗平均观察值的数据集。在将研究视为随机效应的同时建立了模型。选择8个因素进行模型开发,包括饲料组成因素,包括成分夹杂物和营养成分含量,以及关键的制造参数。所有模型均采用3次交叉验证方法进行评估,将整个研究分成几次。结果表明,采用脂质(g/kg)和蛋白质(g/kg)的双变量模型对PDI的预测效果最好,其一致性相关系数为0.57 ± 0.076,偏倚校正系数为0.91 ± 0.092,Pearson相关系数为0.63 ± 0.137。这项荟萃分析不仅提供了一个基于一组统计标准选择模型的管道,而且还产生了简单的模型,可以在饲料生产中用于在数据可用性有限的情况下估计PDI。
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引用次数: 0
Use of microencapsulated pepper with different levels of energy supplementation in grazing beef cattle 微胶囊辣椒在放牧肉牛体内不同能量补充水平的应用
IF 2.7 2区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2025-11-04 DOI: 10.1016/j.anifeedsci.2025.116564
Ana Paula Gonçalves Ferreira Miranda , Gleidson Giordano Pinto de Carvalho , Douglas dos Santos Pina , Leandro Soares Santos , Lara Maria Santos Brant , Paula Velozo Leal , Aureliano José Vieira Pires , Raiane Barbosa Mendes , Evandro Pereira Neto , Mirella Matos de Oliveira Xavier , Caio Seiti Takiya , José Esler de Freitas Júnior
The objective of this study was to evaluate the effects of microencapsulated hot chili pepper (MCP) and different energy supplementation levels on nutrient intake and digestibility, ingestive behavior, ruminal fermentation, nitrogen and energy balance, and physiological parameters of grazing Nellore bulls. Six rumen-cannulated Nellore bull (313 ± 31 kg body weight (BW)) were distributed in a double 3 × 3 Latin square design with a 3 × 2 factorial treatment arrangement and 21-day experimental periods (14 days of adaptation and 7 days of data collection). The animals were housed in a pen on Pangola grass pasture (Digitaria decumbens). Treatments consisted of three energy supplementation levels (SUP) (0.15, 0.30, and 0.45 % of BW) with or without MCP inclusion (450 mg/animal/day of Capsicum sp). MCP inclusion did not affect nutrient intake or digestibility (P > 0.05). Increasing energy supplementation levels led to greater intake and digestibility of non-fiber carbohydrates (P < 0.01) and reduced ruminal ammonia nitrogen concentrations (P < 0.01). The inclusion of MCP increased rumen propionate concentration (P < 0.01) and increased heart rate (P = 0.01). An interaction effect between SUP and MCP was observed for the C2:C3 ratio (P = 0.04). Animals fed 0.15 % SUP with MCP and 0.30 % SUP without MCP presented a higher ratio than those supplemented with 0.30 % MCP and 0.45 % with or without MCP. The inclusion of microencapsulated chili pepper in the diet of grazing beef cattle, combined with intermediate levels of energy supplementation, is a strategy to promote adjustments in ruminal fermentation and improve energy use efficiency.
本试验旨在研究微囊化辣椒(MCP)和不同能量添加水平对放牧Nellore公牛营养物质摄入和消化率、摄食行为、瘤胃发酵、氮和能量平衡以及生理参数的影响。选取体重313 ± 31 kg的瘤胃空心公牛6头,采用双3 × 3拉丁方试验设计,采用3 × 2因子处理,试验期21 d(预试期14 d,数据收集期7 d)。这些动物被安置在Pangola草地(Digitaria decumbens)的围栏内。各组分别添加或不添加MCP(450 mg/只/天辣椒sp)的三种能量补充水平(0.15、0.30和0.45 %体重)。MCP不影响营养摄入和消化率(P > 0.05)。增加能量补充水平提高了非纤维碳水化合物的摄入量和消化率(P <; 0.01),降低了瘤胃氨氮浓度(P <; 0.01)。MCP使瘤胃丙酸浓度升高(P <; 0.01),心率升高(P = 0.01)。SUP和MCP对C2:C3比值有交互作用(P = 0.04)。饲粮中添加MCP的0.15 % SUP和不添加MCP的0.30 % SUP高于饲粮中添加或不添加MCP的0.30 % SUP和0.45 % SUP的动物。在放牧肉牛饲粮中添加微囊化辣椒,并在饲粮中添加中等水平的能量补充,可促进瘤胃发酵调节,提高能量利用效率。
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引用次数: 0
Effect of drying method for dry matter determination on losses of fermentation compounds in mixed beet silages 干物质测定干燥法对混合甜菜青贮发酵化合物损失的影响
IF 2.7 2区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2025-11-04 DOI: 10.1016/j.anifeedsci.2025.116565
Trinidad de Evan , Emma Marie Vallentin Hvas , Mogens Larsen , Martin R. Weisbjerg
The dry matter (DM) content of silage that encompasses all organic matter content is underestimated when determined by conventional methods due to the evaporation of volatile organic compounds (VOC). In the case of beet silage, the high sugar content in beets can accentuate VOC evaporation; therefore, the objective of this experiment was to study the losses of acetic acid, DL-lactate acid, and ethanol from mixed beet silage samples during freeze-drying or oven-drying to assess their volatilization and the appropriateness of using a correction equation for beet silages. Seven different mixed beet silages were freeze-dried or oven-dried at 50 and 60 ºC (24 h), or at 70, 80, and 100 ºC (6 h). The DM was measured, and the VOC were determined in the wet and dried samples to calculate corrected DM in two different ways: using an equation based on silage concentration of VOC or by adding the VOC losses directly to the DM obtained. Interactions between the drying method and the type of silage were found (P < 0.01) for the pH, acetic acid, DL-lactic acid, and ethanol content, as well as for their losses during drying. Significant differences (P < 0.05) were found for all the silages for all the drying methods between the DM determined and both corrected DM. Thus, it is necessary to consider the drying methodology and the chemical composition of silages to evaluate their true DM and organic matter content. Despite these differences, the evaluated equation for correcting DM in beet silages was acceptable and showed reasonable robustness across various oven-drying temperatures.
由于挥发性有机化合物(VOC)的蒸发,用传统方法测定青贮的干物质(DM)含量(包括所有有机物含量)被低估了。在甜菜青贮的情况下,甜菜含糖量高会加重VOC的蒸发;因此,本实验的目的是研究混合甜菜青贮样品在冷冻干燥或烘箱干燥过程中醋酸、dl -乳酸和乙醇的损失,以评估其挥发性以及使用甜菜青贮修正方程的适用性。7种不同的混合甜菜青贮料在50和60℃(24 h)或70、80和100℃(6 h)下冷冻干燥或烘箱干燥。测量DM,并测定湿样和干样中的VOC,以两种不同的方法计算修正DM:使用基于青贮VOC浓度的方程或将VOC损失直接添加到得到的DM中。青贮饲料的pH、乙酸、dl -乳酸和乙醇含量及其在干燥过程中的损失与青贮饲料类型之间存在交互作用(P <; 0.01)。所有干燥方法下的青贮在测定的干物质与两种修正的干物质之间存在显著差异(P <; 0.05)。因此,有必要考虑干燥方法和青贮的化学成分来评估其真实干物质和有机质含量。尽管存在这些差异,但校正甜菜青贮DM的评估方程是可以接受的,并且在不同的烘箱干燥温度下表现出合理的稳健性。
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引用次数: 0
Twice daily feeding of two phytogenic dietary additives; their individual and combined effects on methane production, dry matter intake, milk production and composition in dairy cows 两种植物性饲料添加剂每日2次饲喂;它们对奶牛甲烷产量、干物质采食量、产奶量和成分的单独和联合影响
IF 2.7 2区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2025-11-04 DOI: 10.1016/j.anifeedsci.2025.116559
C. Adam , I. Dando , M.J. Berkhout , A.S. ó Neachtain , C.K.M. Ho , J.L. Jacobs , K. Giri , S.R.O. Williams , L.C. Marett
Plant secondary compounds are sometimes reported to reduce methane (CH4) emissions in ruminants. We assessed the impacts of feeding essential oil-rich Agolin and polyphenol-rich Polygain to dairy cows. Sixty multiparous Holstein-Friesian cows in late-lactation were randomly assigned to one of four treatments: CON, basal diet alone; AGN, basal diet plus 1 g/cow per day of Agolin; PGN, basal diet plus 0.25 % DMI of Polygain; or AGN+PGN, basal diet plus 1 g/cow per day of Agolin and 0.25 % DMI of Polygain. Treatments were offered twice daily during milking. The basal diet was ad libitum vetch hay (Vicia sativa L.) and 7 kg DM of a grain mix. Cows were adapted to the treatments for 28 days, then CH4 emissions were measured using the modified SF6 tracer technique for five days. Compared to CON, there was no difference in total DMI (kg DM/d), CH4 yield (g/kg DMI) or milk yield (kg/d) for any treatment. Cows offered the AGN+PGN diet had a numerically greater CH4 production than the CON cows. Economic analysis considered the benefit from milk production and the value of reduced CH4 production based on numerical differences between treatments. From this, the AGN diet resulted in the greatest total net benefit of A$0.91/cow per day above the CON diet. Our results are contrary to some previous research, but our feeding frequency and method of assessing CH4 emissions were also different. For comparison with previous work, future research should consider the feeding frequency, duration of feeding, and method of CH4 measurement.
植物次生化合物有时被报道可以减少反刍动物的甲烷(CH4)排放。本研究评估了饲喂富含精油的Agolin和富含多酚的Polygain对奶牛的影响。60头哺乳期晚期的多产荷斯坦-弗里西亚奶牛随机分为4组:CON,单纯基础饲粮;AGN,基础日粮加1 g/奶牛/天的Agolin;PGN,基础日粮加0.25 % DMI Polygain;或AGN+PGN,基础饲粮中添加1 g/奶牛/天的Agolin和0.25 % DMI的Polygain。在挤奶期间每天进行两次治疗。基础饲粮为自由采食的紫薇干草和7 kg DM的杂粮混合。奶牛适应处理28 d后,采用改良的SF6示踪技术测量5 d的CH4排放量。与对照组相比,各处理对总DMI (kg DM/d)、CH4产量(g/kg DMI)和产奶量(kg/d)均无显著影响。提供AGN+PGN饲粮的奶牛的CH4产量在数值上高于CON奶牛。经济分析考虑了产奶的效益和基于处理之间数值差异的减少CH4产量的价值。由此可见,AGN日粮比CON日粮的总净效益最高,为每头奶牛每天0.91澳元。我们的研究结果与以往的一些研究结果相反,但我们的饲养频率和评估CH4排放的方法也有所不同。为了与以往的工作进行比较,未来的研究应考虑饲养频率、饲养持续时间和CH4测量方法。
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引用次数: 0
Foxtail millet bran as a functional feed ingredient: Effects on growth, feed efficiency, and nutrient digestibility in grass carp (Ctenopharyngodon idella) 谷糠作为功能性饲料原料对草鱼生长、饲料效率和营养物质消化率的影响
IF 2.7 2区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2025-11-04 DOI: 10.1016/j.anifeedsci.2025.116563
Aditi Banik , Shivendra Kumar , Maneesh Kumar Dubey , R.K. Brahmchari , Sujit Kumar Nayak , Prem Prakash Srivastava
A 60-day feeding trial was conducted to assess the effects of dietary foxtail millet (Setaria italica) bran (FMB) on growth performance, feed utilization, and nutrient digestibility in grass carp (Ctenopharyngodon idella) fingerlings. Five experimental diets were formulated to be isonitrogenous (30 % crude protein), isolipidic (6 % crude lipid), and isoenergetic (380 Kcal/100 g), incorporating graded levels of FMB: 0 % (control, C), 15 % (T15), 30 % (T30), 45 % (T45), and 60 % (T60). A total of 200 fingerlings (average weight 3.18 ± 0.03 g) were randomly distributed into 20 tanks (10 fish per tank) in quadruplicate groups under a completely randomized design. At the conclusion of the trial, growth performance increased significantly (P < 0.05) with dietary FMB inclusion up to 30 %, with the T30 group showing the highest WG (%) and SGR, both significantly greater than those of all other treatments. Polynomial regression analysis (y = -687.32x² + 401.57x + 80.532, R² = 0.845) indicated an optimal WG% at 30 % inclusion. Similarly, RNA/DNA ratios and apparent digestibility of dry matter, protein, lipid, and carbohydrate were highest in T30 but declined markedly in T45 and T60 groups. Digestive enzyme activity also followed this pattern, peaking at 30 % inclusion and decreasing progressively at higher levels, with the lowest activities observed in T60. While serum glucose concentrations remained unaffected up to 30 % inclusion, elevated levels were recorded at 45 % and 60 %. Overall, the findings demonstrate that FMB can be effectively incorporated into the diet of grass carp fingerlings at up to 30 % without adverse effects on growth, nutrient utilization, digestive physiology, or serum biochemistry.
本试验旨在研究饲粮中添加谷子糠(FMB)对草鱼鱼苗生长性能、饲料利用率和营养物质消化率的影响。5种试验饲粮分别为等氮(30 %粗蛋白质)、等脂(6 %粗脂肪)和等能(380 Kcal/100 g), FMB水平分别为0 %(对照,C)、15 % (T15)、30 % (T30)、45 % (T45)和60 % (T60)。采用完全随机设计,将200尾平均体重为3.18 ± 0.03 g的鱼种随机分为4个重复组,每箱10尾。试验结束时,当饲料中FMB添加量达到30% %时,生长性能显著提高(P <; 0.05),其中T30组的增重(%)和SGR最高,均显著高于其他处理。多项式回归分析(y = -687.32x²+ 401.57x + 80.532, R²= 0.845)表明,最佳的WG%为30 %。同样,干物质、蛋白质、脂肪和碳水化合物的RNA/DNA比率和表观消化率在T30组最高,但在T45和T60组显著下降。消化酶活性也遵循这一模式,在30% %时达到峰值,随着添加量的增加逐渐下降,在T60时活性最低。虽然血清葡萄糖浓度在30% %前保持不受影响,但在45% %和60% %时记录到升高的水平。综上所述,本试验结果表明,在草鱼鱼种饲料中添加高达30% %的FMB对其生长、养分利用、消化生理和血清生化均无不良影响。
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Animal Feed Science and Technology
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