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Animal Species Classification from Vocalizations Using Cochlear-Inspired Audio Features and Machine Learning. 利用耳蜗启发的音频特征和机器学习从发声中分类动物物种。
IF 3.9 3区 医学 Q1 ENGINEERING, MULTIDISCIPLINARY Pub Date : 2025-12-11 DOI: 10.3390/biomimetics10120830
Karim Youssef, Julien Moussa H Barakat, Ghina El Mir, Sherif Said, Samer Al Kork, Alaa Eleyan

Biomimetic approaches have gained increasing attention in the development of efficient computational models for sound scene analysis. In this paper, we present a sound-based animal species classification method inspired by the auditory processing mechanisms of the human cochlea. The approach employs gammatone filtering to extract features that capture the distinctive characteristics of animal vocalizations. While gammatone filterbanks themselves are well established in auditory signal processing, their systematic application and evaluation for animal vocalization classification represent the main contribution of this work. Four gammatone-based feature representations are explored and used to train and test an artificial neural network for species classification. The method is evaluated on a dataset comprising vocalizations from 13 animal species with 50 vocalizations per specie and 2.76 seconds per vocalization in average. The evaluations are conducted to study the system parameters in different conditions and system architectures. Although the dataset is limited in scale compared to larger public databases, the results highlight the potential of combining biomimetic cochlear filtering with machine learning to perform reliable and robust species classification through sound.

仿生方法在声音场景分析的高效计算模型的发展中得到越来越多的关注。在本文中,我们提出了一种基于声音的动物物种分类方法,该方法受到了人类耳蜗听觉加工机制的启发。该方法采用伽玛酮过滤来提取捕捉动物发声特征的特征。虽然伽玛酮滤波器组本身在听觉信号处理中已经很好地建立起来,但它们在动物发声分类中的系统应用和评估是这项工作的主要贡献。探索了四种基于伽玛素的特征表示,并用于训练和测试用于物种分类的人工神经网络。该方法在包含13种动物发声的数据集上进行了评估,每种动物发声50次,平均每次发声2.76秒。对不同条件和系统架构下的系统参数进行了评估。尽管与大型公共数据库相比,该数据集的规模有限,但结果强调了将仿生耳蜗过滤与机器学习相结合,通过声音进行可靠和稳健的物种分类的潜力。
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引用次数: 0
Nanomechanical and Optical Properties of Anti-Counterfeiting Nanostructures Obtained by Hydrogel Photoresist in Laser Processing. 水凝胶光刻胶激光防伪纳米结构的纳米力学和光学性能。
IF 3.9 3区 医学 Q1 ENGINEERING, MULTIDISCIPLINARY Pub Date : 2025-12-11 DOI: 10.3390/biomimetics10120829
Wei Wu, Qingxue Deng, Yuhang Shi, Jiyu Sun

The microstructures of living creatures are widely used in bionics, and some can generate structural colors on biological surfaces and enable the process of dynamic camouflage. This study presents the hydrogel photoresist synthesized by polymerizing HEMA and MMA in THF solvent with initiator AIBN. Then, nanostructured gratings were fabricated on the hydrogel photoresists via double-beam interference lithography, and were characterized by scanning electron microscopy, angle-resolved spectroscopy system, and nanoindentation for pattern characterization, and nanomechanical and optical performance, respectively. Under multi-angle incident light, the optical computation of gratings with different depths indicates that a shallow implicit grating does not affect its dynamic color-changing performance. It is established that the laser power of 500 mW, a first exposure time of 5 s, and a second exposure time of 3 s are feasible for achieving efficient anti-counterfeiting nanostructures. The L500-5-3 has greater Er and H than that of L500-5 with the second processing, but smaller than ineffective patterns. And the depth of anti-counterfeiting gratings that is less than 0.8 μm is conducive to obtaining anti-counterfeiting gratings with different size parameters. The acquired anti-counterfeiting nanostructures exhibit excellent stability, reliability, and angle-dependent color changes under room light, which provides promising applications for security materials in daily life, sensors, optics, and electronics.

生物的微结构在仿生学中有着广泛的应用,一些微结构可以在生物表面产生结构色彩,实现动态伪装的过程。以四氢呋喃为溶剂,以AIBN为引发剂,将HEMA和MMA聚合合成水凝胶光胶。然后,利用双光束干涉光刻技术在水凝胶光刻胶上制备纳米结构光栅,并利用扫描电子显微镜、角度分辨光谱系统、纳米压痕模式表征、纳米力学性能和光学性能对其进行表征。在多角度入射光下,对不同深度光栅的光学计算表明,浅隐式光栅不影响其动态变色性能。研究结果表明,激光功率为500 mW,第一次曝光时间为5 s,第二次曝光时间为3 s,可以实现高效防伪纳米结构。二次加工后的L500-5-3的Er和H值大于L500-5,但小于无效模式。防伪光栅深度小于0.8 μm有利于获得不同尺寸参数的防伪光栅。所获得的防伪纳米结构在室内光线下表现出优异的稳定性、可靠性和角度依赖的颜色变化,为日常生活、传感器、光学和电子领域的防伪材料提供了有前途的应用。
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引用次数: 0
Performance of Hammerstein Spline Adaptive Filtering Based on Fair Cost Function for Denoising Electrocardiogram Signals. 基于公平代价函数的Hammerstein样条自适应滤波对心电图信号去噪的性能。
IF 3.9 3区 医学 Q1 ENGINEERING, MULTIDISCIPLINARY Pub Date : 2025-12-10 DOI: 10.3390/biomimetics10120828
Suchada Sitjongsataporn, Theerayod Wiangtong

This paper proposes a simplified adaptive filtering approach using a Hammerstein function and the spline interpolation based on a Fair cost function for denoising electrocardiogram (ECG) signals. The use of linear filters in real-world applications has many limitations. Adaptive nonlinear filtering is a key development in tackling the challenge of discovering the specific characteristics of biomimetic systems for each person in order to eliminate unwanted signals. A biomimetic system refers to a system that mimics certain biological processes or characteristics of the human body, in this case, the individual features of a person's cardiac signals (ECG). Here, the adaptive nonlinear filter is designed to cope with ECG variations and remove unwanted noise more effectively. The objective of this paper is to explore an individual biomedical filter based on adaptive nonlinear filtering for denoising the corrupted ECG signal. The Hammerstein spline adaptive filter (HSAF) architecture consists of two structural blocks: a nonlinear block connected to a linear one. In order to make a smooth convergence, the Fair cost function is introduced for convergence enhancement. The affine projection algorithm (APA) based on the Fair cost function is used to denoise the contaminated ECG signals, and also provides fast convergence. The MIT-BIH 12-lead database is used as the source of ECG biomedical signals contaminated by random noises modelled by Cauchy distribution. Experimental results show that the estimation error of the proposed HSAF-APA-Fair algorithm, based on the Fair cost function, can be reduced when compared with the conventional least mean square-based algorithm for denoising ECG signals.

本文提出了一种简化的自适应滤波方法,利用Hammerstein函数和基于公平代价函数的样条插值对心电图信号进行去噪。在实际应用中使用线性滤波器有许多限制。自适应非线性滤波是解决发现每个人的仿生系统的具体特征以消除不需要的信号的挑战的关键发展。仿生系统是指模仿人体某些生物过程或特征的系统,在这种情况下,模仿人的心脏信号(ECG)的个体特征。本文设计了自适应非线性滤波器来应对心电信号的变化,并更有效地去除不必要的噪声。本文的目的是探索一种基于自适应非线性滤波的个体生物医学滤波器,用于去噪干扰的心电信号。Hammerstein样条自适应滤波器(HSAF)结构由两个结构块组成:一个非线性块与一个线性块相连。为了使算法平滑收敛,引入了Fair代价函数增强收敛性。采用基于Fair代价函数的仿射投影算法(APA)对污染心电信号进行去噪,具有较快的收敛性。利用MIT-BIH 12导联数据库作为受柯西分布随机噪声污染的心电生物医学信号源。实验结果表明,与传统的基于最小均方的心电信号去噪算法相比,基于Fair代价函数的HSAF-APA-Fair算法的估计误差可以降低。
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引用次数: 0
Energy-Efficient Path Planning for Snake Robots Using a Deep Reinforcement Learning-Enhanced A* Algorithm. 基于深度强化学习增强a *算法的蛇形机器人节能路径规划。
IF 3.9 3区 医学 Q1 ENGINEERING, MULTIDISCIPLINARY Pub Date : 2025-12-10 DOI: 10.3390/biomimetics10120826
Yang Gu, Zelin Wang, Zhong Huang

Snake-like robots, characterized by their high flexibility and multi-joint structure, exhibit exceptional adaptability to complex terrains such as snowfields, jungles, deserts, and underwater environments. Their ability to navigate narrow spaces and circumvent obstacles makes them ideal for operations in confined or rugged environments. However, efficient motion in such conditions requires not only mechanical flexibility but also effective path planning to ensure safety, energy efficiency, and overall task performance. Most existing path planning algorithms for snake-like robots focus primarily on finding the shortest path between the start and target positions while neglecting the optimization of energy consumption during real operations. To address this limitation, this study proposes an energy-efficient path planning method based on an improved A* algorithm enhanced with deep reinforcement learning: Dueling Double-Deep Q-Network (D3QN). An Energy Consumption Estimation Model (ECEM) is first developed to evaluate the energetic cost of snake robot motion in three-dimensional space. This model is then integrated into a new heuristic function to guide the A* search toward energy-optimal trajectories. Simulation experiments were conducted in a 3D environment to assess the performance of the proposed approach. The results demonstrate that the improved A* algorithm effectively reduces the energy consumption of the snake robot compared with conventional algorithms. Specifically, the proposed method achieves an energy consumption of 68.79 J, which is 3.39%, 27.26%, and 5.91% lower than that of the traditional A* algorithm (71.20 J), the bidirectional A* algorithm (94.61 J), and the weighted improved A* algorithm (73.11 J), respectively. These findings confirm that integrating deep reinforcement learning with an adaptive heuristic function significantly enhances both the energy efficiency and practical applicability of snake robot path planning in complex 3D environments.

蛇形机器人具有高灵活性和多关节结构的特点,对雪地、丛林、沙漠和水下环境等复杂地形表现出卓越的适应性。他们的能力导航狭窄的空间和规避障碍,使他们在狭窄或崎岖的环境中操作的理想选择。然而,在这种情况下,高效的运动不仅需要机械灵活性,还需要有效的路径规划,以确保安全、能源效率和整体任务性能。现有的蛇形机器人路径规划算法大多侧重于寻找起始位置和目标位置之间的最短路径,而忽略了实际操作过程中能量消耗的优化。为了解决这一限制,本研究提出了一种基于深度强化学习增强的改进A*算法的节能路径规划方法:Dueling Double-Deep Q-Network (D3QN)。首先建立了蛇形机器人在三维空间中运动的能量消耗估算模型(ECEM)。然后将该模型集成到一个新的启发式函数中,以指导a *搜索朝向能量最优轨迹。在三维环境下进行了仿真实验,以评估所提出方法的性能。结果表明,与传统算法相比,改进的A*算法有效地降低了蛇形机器人的能量消耗。具体而言,该方法的能量消耗为68.79 J,分别比传统A*算法(71.20 J)、双向A*算法(94.61 J)和加权改进A*算法(73.11 J)低3.39%、27.26%和5.91%。这些研究结果证实,将深度强化学习与自适应启发式函数相结合,可以显著提高复杂3D环境下蛇形机器人路径规划的能效和实用性。
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引用次数: 0
Hybrid ANFIS-MPA and FFNN-MPA Models for Bitcoin Price Forecasting. 比特币价格预测的混合anfiss - mpa和FFNN-MPA模型。
IF 3.9 3区 医学 Q1 ENGINEERING, MULTIDISCIPLINARY Pub Date : 2025-12-10 DOI: 10.3390/biomimetics10120827
Ceren Baştemur Kaya, Ebubekir Kaya, Eyüp Sıramkaya

This study introduces two hybrid forecasting models that integrate the Marine Predators Algorithm (MPA) with Adaptive Neuro-Fuzzy Inference Systems (ANFIS) and Feed-Forward Neural Networks (FFNN) for short-term Bitcoin price prediction. Daily Bitcoin data from 2022 were converted into supervised time-series structures with multiple input configurations. The proposed hybrid models were evaluated against six well-known metaheuristic algorithms commonly used for training intelligent forecasting systems. The results show that MPA consistently yields lower prediction errors, faster convergence, and more stable optimization behavior compared with alternative algorithms. Both ANFIS-MPA and FFNN-MPA maintained their advantage across all tested structures, demonstrating reliable performance under varying model complexities. All experiments were repeated multiple times, and the hybrid approaches exhibited low variance, indicating robust and reproducible behavior. Overall, the findings highlight the effectiveness of MPA as an optimizer for improving the predictive performance of neuro-fuzzy and neural network models in financial time-series forecasting.

本研究引入了两种混合预测模型,将海洋捕食者算法(MPA)与自适应神经模糊推理系统(ANFIS)和前馈神经网络(FFNN)相结合,用于短期比特币价格预测。将2022年以来的每日比特币数据转换为具有多个输入配置的监督时间序列结构。根据六种常用的用于训练智能预测系统的元启发式算法对所提出的混合模型进行了评估。结果表明,与其他算法相比,MPA的预测误差更小,收敛速度更快,优化行为更稳定。anfiss - mpa和FFNN-MPA在所有测试结构中都保持了优势,在不同的模型复杂性下表现出可靠的性能。所有实验重复多次,混合方法表现出低方差,表明鲁棒性和可重复性。总体而言,研究结果突出了MPA作为优化器的有效性,可以提高神经模糊和神经网络模型在金融时间序列预测中的预测性能。
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引用次数: 0
Human-Inspired Force-Motion Imitation Learning with Dynamic Response for Adaptive Robotic Manipulation. 基于动态响应的自适应机器人仿生力运动模仿学习。
IF 3.9 3区 医学 Q1 ENGINEERING, MULTIDISCIPLINARY Pub Date : 2025-12-09 DOI: 10.3390/biomimetics10120825
Yuchuang Tong, Haotian Liu, Tianbo Yang, Zhengtao Zhang

Recent advances in bioinspired robotics highlight the growing demand for dexterous, adaptive control strategies that allow robots to interact naturally, safely, and efficiently with dynamic, contact-rich environments. Yet, achieving robust adaptability and reflex-like responsiveness to unpredictable disturbances remains a fundamental challenge. This paper presents a bioinspired imitation learning framework that models human adaptive dynamics to jointly acquire and generalize motion and force skills, enabling compliant and resilient robot behavior. The proposed framework integrates hybrid force-motion learning with dynamic response mechanisms, achieving broad skill generalization without reliance on external sensing modalities. A momentum-based force observer is combined with dynamic movement primitives (DMPs) to enable accurate force estimation and smooth motion coordination, while a broad learning system (BLS) refines the DMP forcing function through style modulation, feature augmentation, and adaptive weight tuning. In addition, an adaptive radial basis function neural network (RBFNN) controller dynamically adjusts control parameters to ensure precise, low-latency skill reproduction, and safe physical interaction. Simulations and real-world experiments confirm that the proposed framework achieves human-like adaptability, robustness, and scalability, attaining a competitive learning time of 5.56 s and a rapid generation time of 0.036 s, thereby demonstrating its efficiency and practicality for real-time applications and offering a lightweight yet powerful solution for bioinspired intelligent control in complex and unstructured environments.

生物机器人技术的最新进展突出了对灵巧、自适应控制策略的日益增长的需求,这些策略使机器人能够自然、安全、高效地与动态、接触丰富的环境进行交互。然而,实现强大的适应性和对不可预测的干扰的反射式响应仍然是一个根本性的挑战。本文提出了一个仿生模仿学习框架,该框架模拟了人类自适应动力学,以共同获取和推广运动和力量技能,从而实现了机器人的柔顺和弹性行为。提出的框架将混合力-运动学习与动态响应机制相结合,在不依赖外部传感模式的情况下实现广泛的技能泛化。基于动量的力观测器与动态运动原语(DMP)相结合,实现准确的力估计和平滑的运动协调,而广义学习系统(BLS)通过风格调制、特征增强和自适应权值调整来改进DMP强迫函数。此外,自适应径向基函数神经网络(RBFNN)控制器动态调整控制参数,以确保精确、低延迟的技能再现和安全的物理交互。仿真和实际实验验证了所提出的框架具有类似人类的适应性、鲁棒性和可扩展性,具有5.56 s的竞争性学习时间和0.036 s的快速生成时间,从而证明了其在实时应用中的效率和实用性,为复杂和非结构化环境中的生物智能控制提供了轻量级而强大的解决方案。
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引用次数: 0
Composites Derived from Aluminium-Modified Biphasic Calcium-Phosphate for Bone Regeneration. 用于骨再生的铝改性双相磷酸钙复合材料。
IF 3.9 3区 医学 Q1 ENGINEERING, MULTIDISCIPLINARY Pub Date : 2025-12-09 DOI: 10.3390/biomimetics10120824
Raluca Lucacel-Ciceo, Roxana Dudric, Razvan Hirian, Iulia Lupan, Oana Koblicska, Roxana Strimbu, Radu George Hategan, Dorina Simedru, Zorita Diaconeasa

In this research, aluminium-doped biphasic calcium phosphate (Al-BCP) was synthesized by co-precipitation and formulated with hydrolyzed collagen and acetylsalicylic acid (ASA) to yield composites designed as a new class of bone-regenerative biomaterials with enhanced biological performance. Undoped and Al-modified powders (5/10 wt% Al precursor) were prepared at 40 °C (pH ~ 11) and calcined at 700 °C, and composites were produced at a 1:1:0.1 mass ratio (ceramic-collagen-ASA). Structure and chemistry were assessed by X-ray diffraction (XRD), Fourier-transform infrared (FTIR) and Raman spectroscopies, and X-ray photoelectron spectroscopy (XPS). Morphology and elemental distribution were examined by scanning electron microscopy/energy-dispersive X-ray spectroscopy (SEM/EDX). Biological performance was preliminarily evaluated using HaCaT (immortalized human keratinocytes) viability and antibacterial assays against Staphylococcus aureus and Escherichia coli. XRD confirmed a biphasic hydroxyapatite/β-tricalcium phosphate system and showed that Al incorporation shifted the phase balance toward hydroxyapatite (HAp fraction 54.8% in BCP vs. ~68.6-68.7% in Al-doped samples). FTIR/Raman preserved BCP vibrational signatures and revealed collagen/ASA bands in the composites. XPS/EDX verified the expected composition, including surface N 1s from organics and Al at ~2-5 at% for doped samples, with surface Ca/P ≈ 1.15-1.16. SEM revealed multigranular microstructures with homogeneous Al distribution. All composites were non-cytotoxic (≥70% viability); M_Al10_Col_ASA exceeded 90% viability at 12.5% dilution. Preliminary antibacterial assays against Gram-positive and Gram-negative strains showed modest, time-dependent reductions in CFU relative to controls. These results corroborate the compositional/structural profile and preliminary biological performance of Al-BCP-collagen-ASA composites as multifunctional bone tissue engineering materials that foster a bone-friendly microenvironment, warranting further evaluation for bone regeneration.

本研究采用共沉淀法合成了掺铝双相磷酸钙(Al-BCP),并与水解胶原蛋白和乙酰水杨酸(ASA)配制成复合材料,设计了一种具有增强生物性能的新型骨再生生物材料。在40℃(pH ~ 11)下制备未掺杂和Al修饰的粉末(5/10 wt% Al前驱体),在700℃下煅烧,以1:1:0.1的质量比制备复合材料(陶瓷-胶原蛋白- asa)。通过x射线衍射(XRD)、傅里叶变换红外(FTIR)和拉曼光谱(Raman)以及x射线光电子能谱(XPS)对其结构和化学性质进行了评价。利用扫描电镜/能量色散x射线能谱(SEM/EDX)对其形貌和元素分布进行了分析。采用永生化人角质形成细胞(HaCaT)活性及对金黄色葡萄球菌和大肠杆菌的抑菌试验对其生物学性能进行初步评价。XRD证实了一个双相羟基磷灰石/β-磷酸三钙体系,并表明Al的掺入使相平衡向羟基磷灰石倾斜(BCP中HAp分数为54.8%,而Al掺杂样品中HAp分数为68.6-68.7%)。FTIR/Raman保留了BCP的振动特征,并在复合材料中发现了胶原/ASA条带。XPS/EDX验证了预期的组成,包括来自有机物的表面N - 1s和掺杂样品中~2-5 at%的Al,表面Ca/P≈1.15-1.16。SEM显示多颗粒组织,Al分布均匀。所有复合材料均无细胞毒性(活性≥70%);在12.5%的稀释度下,M_Al10_Col_ASA存活率超过90%。对革兰氏阳性和革兰氏阴性菌株的初步抗菌试验显示,相对于对照组,CFU有适度的、时间依赖性的降低。这些结果证实了al - bcp -胶原- asa复合材料作为多功能骨组织工程材料的组成/结构特征和初步生物学性能,可培养骨友好微环境,值得进一步评估骨再生。
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引用次数: 0
Development of a Bayesian Network and Information Gain-Based Axis Dynamic Mechanism for Ankle Joint Rehabilitation. 基于贝叶斯网络和信息增益的踝关节康复轴动力机制研究。
IF 3.9 3区 医学 Q1 ENGINEERING, MULTIDISCIPLINARY Pub Date : 2025-12-09 DOI: 10.3390/biomimetics10120823
Huiguo Ma, Yuqi Bao, Jingfu Lan, Xuewen Zhu, Pinwei Wan, Raquel Cedazo León, Shuo Jiang, Fangfang Chen, Jun Kang, Qihan Guo, Peng Zhang, He Li

In response to the personalized and precise rehabilitation needs for motor injuries and stroke associated with population aging, this study proposes a design method for an intelligent rehabilitation trainer that integrates Bayesian information gain (BIG) and axis matching techniques. Grounded in the biomechanical characteristics of the human ankle joint, the design fully draws upon biomimetic principles, constructing a 3-PUU-R hybrid serial-parallel bionic mechanism. By mimicking the dynamic variation of the ankle's instantaneous motion axis and its balance between stiffness and compliance, a three-dimensional digital model was developed, and multi-posture human factor simulations were conducted, thereby achieving a rehabilitation process more consistent with natural human movement patterns. Natural randomized disability grade experimental data were collected for 100 people to verify the validity of the design results. On this basis, a Bayesian information gain framework was established by quantifying the reduction of uncertainty in rehabilitation outcomes through characteristic parameters, enabling the dynamic optimization of training strategies for personalized and precise ankle rehabilitation. The rehabilitation process was modeled as a problem of uncertainty quantification and information gain optimization. Prior distributions were constructed using surface EMG (electromyography) signals and motion trajectory errors, and mutual information was used to drive the dynamic adjustment of training strategies, ultimately forming a closed-loop control architecture of "demand perception-strategy optimization-execution adaptation." This innovative integration of probabilistic modeling and cross-joint bionic design overcomes the limitations of single-joint rehabilitation and provides a new paradigm for the development of intelligent rehabilitation devices. The deep integration mechanism-based dynamic axis matching and Bayesian information gain holds significant theoretical value and engineering application prospects for enhancing the effectiveness of neural plasticity training.

针对人口老龄化相关运动损伤和脑卒中的个性化精准康复需求,本研究提出了一种融合贝叶斯信息增益(BIG)和轴匹配技术的智能康复训练器设计方法。本设计立足于人体踝关节的生物力学特点,充分借鉴仿生原理,构建了3-PUU-R混合型串并联仿生机构。通过模拟踝关节瞬时运动轴的动态变化及其在刚度和柔顺性之间的平衡,建立三维数字模型,进行多姿态人为因素仿真,从而实现更符合人体自然运动模式的康复过程。收集100人的自然随机残疾等级实验数据,验证设计结果的有效性。在此基础上,通过特征参数量化减少康复结果的不确定性,建立贝叶斯信息增益框架,实现个性化精准踝关节康复训练策略的动态优化。将康复过程建模为不确定性量化和信息增益优化问题。利用表面肌电信号和运动轨迹误差构建先验分布,利用互信息驱动训练策略动态调整,最终形成“需求感知-策略优化-执行适应”的闭环控制体系。这种创新性地将概率建模与交叉关节仿生设计相结合,克服了单关节康复的局限性,为智能康复装置的发展提供了新的范式。基于动态轴匹配和贝叶斯信息增益的深度融合机制对于提高神经可塑性训练的有效性具有重要的理论价值和工程应用前景。
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引用次数: 0
Chitosan-Based Nanoparticles and Biomaterials for Pulp Capping and Regeneration: A Systematic Review with Quantitative and Evidence-Mapping Synthesis. 壳聚糖纳米颗粒和生物材料用于牙髓覆盖和再生:定量和证据图谱合成的系统综述。
IF 3.9 3区 医学 Q1 ENGINEERING, MULTIDISCIPLINARY Pub Date : 2025-12-09 DOI: 10.3390/biomimetics10120822
Saleh Ali Alqahtani, Mohammad Alamri, Ghadeer Alwadai, Naif N Abogazalah, Vinod Babu Mathew, Betsy Joseph

Preserving dental pulp vitality is a key goal in minimally invasive dentistry. Conventional materials such as calcium hydroxide and mineral trioxide aggregate (MTA) are effective but limited in bioactivity and mechanical strength. This systematic review evaluated the biological efficacy of chitosan-based nanoparticles and biomaterials for pulp capping and regeneration. Following PRISMA 2020 guidelines, electronic searches were conducted across five databases up to April 2025. Controlled in vitro and animal studies using chitosan-based nanoparticles, hydrogels, or composite scaffolds were included. Risk of bias was assessed using SYRCLE (animal) and ToxRTool (in vitro), and certainty of evidence was rated via the GRADE-Preclinical framework. Due to methodological heterogeneity, data were synthesized using direction-of-effect coding and visualized through Albatross and heatmap plots. Sixteen studies met the criteria, consistently demonstrating enhanced cell viability, mineralization, and upregulation of odontogenic and angiogenic markers (BMP-2, TGF-β1, VEGF, DSPP) compared with MTA or calcium hydroxide. Animal models confirmed improved angiogenesis, reparative dentin formation, and pulp vitality preservation. Despite uniformly positive biological outcomes, the overall certainty was rated Low to Very Low owing to small samples and unclear randomization. Chitosan-based biomaterials show promising regenerative potential, warranting well-designed preclinical and clinical studies for translational validation.

保持牙髓活力是微创牙科的一个关键目标。传统的材料,如氢氧化钙和矿物三氧化物骨料(MTA)是有效的,但在生物活性和机械强度方面有限。本系统综述评价了壳聚糖纳米颗粒和生物材料在牙髓覆盖和再生中的生物学功效。根据PRISMA 2020指南,到2025年4月,在五个数据库中进行了电子搜索。使用壳聚糖基纳米颗粒、水凝胶或复合支架的体外和动物对照研究包括在内。使用sycle(动物)和ToxRTool(体外)评估偏倚风险,并通过grade -临床前框架评估证据的确定性。由于方法的异质性,数据采用效果方向编码合成,并通过信天翁图和热图进行可视化。16项研究符合标准,与MTA或氢氧化钙相比,一致显示细胞活力增强,矿化,牙源性和血管生成标志物(BMP-2, TGF-β1, VEGF, DSPP)上调。动物模型证实了血管生成、修复性牙本质形成和牙髓活力保存的改善。尽管生物学结果一致为阳性,但由于样本小和随机化不明确,总体确定性被评为低至极低。壳聚糖基生物材料显示出良好的再生潜力,需要精心设计的临床前和临床研究来进行转化验证。
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引用次数: 0
Bioactive Glass Modified by Sonochemistry Improves Peri-Implant Bone Repair in Ovariectomized Rats. 超声化学修饰生物活性玻璃促进去卵巢大鼠种植体周围骨修复。
IF 3.9 3区 医学 Q1 ENGINEERING, MULTIDISCIPLINARY Pub Date : 2025-12-08 DOI: 10.3390/biomimetics10120821
Marcelly Braga Gomes, Nathália Dantas Duarte, Gabriel Mulinari-Santos, Fábio Roberto de Souza Batista, Luy de Abreu Costa, Paulo Roberto Botacin, Paulo Noronha Lisboa-Filho, Roberta Okamoto

Estrogen deficiency is a primary cause of osteoporosis, compromising bone mineral density that may impair peri-implant healing. Given the compromised bone environment associated with estrogen deficiency, strategies such as particle reduction via sonochemistry are promising approaches to enhance regenerative outcomes. However, its effects in promoting bone formation remain insufficiently explored. Therefore, this study evaluated the potential of two sonicated biomaterials to improve peri-implant repair in ovariectomized rats. Fifty female rats were allocated into five groups: blood clot (CLOT), Biogran® (BGN), sonicated Biogran® (BGS), Bio-Oss® (BON), and sonicated Bio-Oss® (BOS). Tibial peri-implant defects were created 30 days after ovariectomy and analyzed 28 days later by removal torque, microcomputed tomography, and confocal microscopy. BGS exhibited the highest removal torque (6.28 Ncm), followed by BON (5.37 Ncm), BOS (3.92 Ncm), BGN (3.15 Ncm), and CLOT (2.58 Ncm). Micro-CT revealed bone volume fraction (BV/TV) values of 8.07% (CLOT), 6.47% (BOS), 6.02% (BGS), 5.55% (BGN), and 2.84% (BON). For the trabecular number (Tb.N), BGS (1.11 mm-1) showed a significant increase compared with BGN (0.69 mm-1), p < 0.05. These findings show that sonochemically modified bioactive glass improves mechanical stability and trabecular microarchitecture under estrogen-deficient conditions. However, further studies are needed to standardize sonication parameters for different biomaterials and expand their translational applicability.

雌激素缺乏是骨质疏松症的主要原因,影响骨矿物质密度,可能损害种植体周围愈合。考虑到与雌激素缺乏相关的骨骼环境受损,通过超声化学减少颗粒等策略是提高再生结果的有希望的方法。然而,其促进骨形成的作用仍未得到充分探讨。因此,本研究评估了两种超声生物材料改善卵巢切除大鼠种植体周围修复的潜力。将50只雌性大鼠分为血凝块(clot)组、Biogran®(BGN)组、超声Biogran®(BGS)组、Bio-Oss®(BON)组和超声Bio-Oss®(BOS)组。胫骨植入体周围缺损在卵巢切除术后30天产生,并在28天后通过移除扭矩、显微计算机断层扫描和共聚焦显微镜进行分析。BGS的去除扭矩最大(6.28 Ncm),其次是BON (5.37 Ncm)、BOS (3.92 Ncm)、BGN (3.15 Ncm)和CLOT (2.58 Ncm)。Micro-CT显示骨体积分数(BV/TV)值为:CLOT为8.07%,BOS为6.47%,BGS为6.02%,BGN为5.55%,BON为2.84%。对于小梁数(Tb)。N), BGS (1.11 mm-1)较BGN (0.69 mm-1)显著升高,p < 0.05。这些发现表明,声化学修饰的生物活性玻璃改善了雌激素缺乏条件下的机械稳定性和小梁微结构。然而,标准化不同生物材料的超声参数并扩大其转化适用性还需要进一步的研究。
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