This pre-experimental study was done on forty nursing personnel posted in the radiology section of a selected hospital of Gurugram National Capital Region using one group pre-test post-test design. The study sample was selected using convenience sampling technique. A structured knowledge questionnaire and observational checklist were used to assess the effectiveness of the educational package regarding occupational health safety. The mean pre-test knowledge score was 16.65 and mean post-test knowledge score was 22.13. Similarly, the mean pre-test practice score was 12.42 and mean post-test practice score came to be 14.70. The calculated ‘t’ value for knowledge score was 37.099 and the practice score was 11.801 at a 0.05 level of significance. The result reported that nurses were aware of radiation safety and protection, even though it would be helpful if their knowledge and practice are apprised regularly.
{"title":"Enhancing the Occupational Health Safety among Radiology Nurses Working in the Hospital of Gurugram, Haryana, India","authors":"R. Deep","doi":"10.21786/bbrc/15.1.27","DOIUrl":"https://doi.org/10.21786/bbrc/15.1.27","url":null,"abstract":"This pre-experimental study was done on forty nursing personnel posted in the radiology section of a selected hospital of Gurugram National Capital Region using one group pre-test post-test design. The study sample was selected using convenience sampling technique. A structured knowledge questionnaire and observational checklist were used to assess the effectiveness of the educational package regarding occupational health safety. The mean pre-test knowledge score was 16.65 and mean post-test knowledge score was 22.13. Similarly, the mean pre-test practice score was 12.42 and mean post-test practice score came to be 14.70. The calculated ‘t’ value for knowledge score was 37.099 and the practice score was 11.801 at a 0.05 level of significance. The result reported that nurses were aware of radiation safety and protection, even though it would be helpful if their knowledge and practice are apprised regularly.","PeriodicalId":9156,"journal":{"name":"Bioscience Biotechnology Research Communications","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44659942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In this present study, a simple, rapid, cheap, sensitive and reproducible HPTLC-MS Method has been developed for the identification of two important bioactive compounds, Sanguinarine and Dihydrosanguinarine in Argemone mexicana Linn seeds. The work further discussed and developed a sensitive HPTLC – MS method to analyse the adulteration and/or contamination of argemone oil in the edible mustard oil by spiking Sanguinarine and Dihydrosanguinarine as biomarkers. The n-hexane: diethyl ether (1:1 v/v) solvent system has been used as an extraction medium to extract the Sanguinarine and Dihydrosanguinarine from the seeds followed by HPLC-MS detection.The CAMAG HPTLC system modules consisted with ATS-4, ADC-2, visualizer-2, TLC scanner-4; Derivatizer and TLC-MS interface-2 have been used for sample application, HPTLC plate development, plate photo documentation, scanning of plate, spraying of derivatization reagent and elution of biomarkers directly from HPTLC plate respectively. The n-hexane: acetone (23:7, v/v) and dragendorff’s reagent has been used as mobile phase and derivatization reagent respectively. The UV- spectra and MS data confirmed the detection of selected biomarkers in the samples/spiked samples. Thus, the work highlights the use of HPTLC-MS to develop simple and routine method for the sensitive detection of Sanguinarine and Dihydrosanguinarine in Argemone mexicana seeds and able to become bases to these biomarkers’ evaluations in other samples such as various edible oils, agro-products, drugs and biomedical products etc.
{"title":"High Performance Thin-Layer Chromatography-Mass Spectrometry Evaluation of Sanguinarine and Dihydrosanguinarine from Argemone mexicana Seeds in Edible Mustard Oil","authors":"Ramakant Yadav","doi":"10.21786/bbrc/15.1.24","DOIUrl":"https://doi.org/10.21786/bbrc/15.1.24","url":null,"abstract":"In this present study, a simple, rapid, cheap, sensitive and reproducible HPTLC-MS Method has been developed for the identification of two important bioactive compounds, Sanguinarine and Dihydrosanguinarine in Argemone mexicana Linn seeds. The work further discussed and developed a sensitive HPTLC – MS method to analyse the adulteration and/or contamination of argemone oil in the edible mustard oil by spiking Sanguinarine and Dihydrosanguinarine as biomarkers. The n-hexane: diethyl ether (1:1 v/v) solvent system has been used as an extraction medium to extract the Sanguinarine and Dihydrosanguinarine from the seeds followed by HPLC-MS detection.The CAMAG HPTLC system modules consisted with ATS-4, ADC-2, visualizer-2, TLC scanner-4; Derivatizer and TLC-MS interface-2 have been used for sample application, HPTLC plate development, plate photo documentation, scanning of plate, spraying of derivatization reagent and elution of biomarkers directly from HPTLC plate respectively. The n-hexane: acetone (23:7, v/v) and dragendorff’s reagent has been used as mobile phase and derivatization reagent respectively. The UV- spectra and MS data confirmed the detection of selected biomarkers in the samples/spiked samples. Thus, the work highlights the use of HPTLC-MS to develop simple and routine method for the sensitive detection of Sanguinarine and Dihydrosanguinarine in Argemone mexicana seeds and able to become bases to these biomarkers’ evaluations in other samples such as various edible oils, agro-products, drugs and biomedical products etc.","PeriodicalId":9156,"journal":{"name":"Bioscience Biotechnology Research Communications","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45128044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Studies report inconclusive results regarding the efficacy of oral corticosteroids to reduce the risk of re-visiting the Emergency Department (ED). The aim of this study was to compare COPD patients who received oral corticosteroids and got re -admitted to the ED earlier than 60 days. An observational study was conducted at the ED from 2016 to 2018. A cohort of adult COPD patients, who received oral corticosteroids was assessed for any ED re-visit due to a COPD exacerbation. A total of 325 COPD patients included the study, 71% had no subsequent ED visit, and 94 (28%) patients had a repeat ED visit due to a COPD exacerbation. Of this ED re-visit group, 61% was within 60 days. The ED re-visit within 60 days group was more likely to have cardiovascular disease than the group with an ED re-visit after 60 days (51% vs. 45%, p-value 0.64).The use of oral corticosteroids could potentially reduce the severity of COPD exacerbation and ED re-visits.
{"title":"Emergency Department Revisit Rate of Chronic Obstructive Pulmonary Disease Patients On Oral Corticosteroids: An Observational Study","authors":"T. Ismaeil","doi":"10.21786/bbrc/15.1.6","DOIUrl":"https://doi.org/10.21786/bbrc/15.1.6","url":null,"abstract":"Studies report inconclusive results regarding the efficacy of oral corticosteroids to reduce the risk of re-visiting the Emergency Department (ED). The aim of this study was to compare COPD patients who received oral corticosteroids and got re -admitted to the ED earlier than 60 days. An observational study was conducted at the ED from 2016 to 2018. A cohort of adult COPD patients, who received oral corticosteroids was assessed for any ED re-visit due to a COPD exacerbation. A total of 325 COPD patients included the study, 71% had no subsequent ED visit, and 94 (28%) patients had a repeat ED visit due to a COPD exacerbation. Of this ED re-visit group, 61% was within 60 days. The ED re-visit within 60 days group was more likely to have cardiovascular disease than the group with an ED re-visit after 60 days (51% vs. 45%, p-value 0.64).The use of oral corticosteroids could potentially reduce the severity of COPD exacerbation and ED re-visits.","PeriodicalId":9156,"journal":{"name":"Bioscience Biotechnology Research Communications","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42077522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
According to the report of World Health Organization, it is expected that depression will be one of the major causes for disability and disease in the world, by 2030. Currently used drugs will not be effective to the depressive patients, due to their lower efficiency with side effects. Hence, there is a need to concentrate on natural products to overcome therapeutic hassles. Morin, a bioflavonoid found in fruits, vegetables, some herbs and wine is reported to have an antidepressant-like effect against acute stress conditions. It is reported to possess various pharmacological properties such as antioxidant, anti-diabetic, hepatoprotective, anti-cancer and anti-inflammatory activities. To understand the antidepressant effect of morin against unpredicted chronic mild stress (UCMS), rats were divided into normal, UCMS alone, UCMS and morin (30 & 60 mg/kg) and morin alone (60 mg/kg) groups. Serum corticosterone levels, expression of brain derived neurotrophic factor (BDNF), apoptotic and endoplasmic reticulum (ER) stress related indices were compared among the groups. UCMS alone exposed rats showed less crossings with diminished activities in open field test (OFT), increased serum corticosterone levels and enhanced expression of BDNF signaling and ER stress related markers associated with apoptosis as compared to control. In contrast, morin (60 mg/kg b.w.) cotreatment attenuated UCMS induced abnormalities as compared to UCMS alone exposed animals. The present results indicated the antidepressant-like actions of morin against UCMS in rats were partially due to its anti-apoptotic effects by regulating BDNF/Trk-B and ER stress related markers. Moreover the results of the present study indicated that the morin may act as therapeutic agent for the management of depression alone or with other currently used antidepressants.
{"title":"Morin mitigates Chronic and Unpredictable Mild Stress-Induced Depression by Regulation of Endoplasmic Reticulum Stress and Brain-Derived Neurotrophic Factor-Mediated Apoptosis","authors":"A. Thenmozhi","doi":"10.21786/bbrc/15.1.29","DOIUrl":"https://doi.org/10.21786/bbrc/15.1.29","url":null,"abstract":"According to the report of World Health Organization, it is expected that depression will be one of the major causes for disability and disease in the world, by 2030. Currently used drugs will not be effective to the depressive patients, due to their lower efficiency with side effects. Hence, there is a need to concentrate on natural products to overcome therapeutic hassles. Morin, a bioflavonoid found in fruits, vegetables, some herbs and wine is reported to have an antidepressant-like effect against acute stress conditions. It is reported to possess various pharmacological properties such as antioxidant, anti-diabetic, hepatoprotective, anti-cancer and anti-inflammatory activities. To understand the antidepressant effect of morin against unpredicted chronic mild stress (UCMS), rats were divided into normal, UCMS alone, UCMS and morin (30 & 60 mg/kg) and morin alone (60 mg/kg) groups. Serum corticosterone levels, expression of brain derived neurotrophic factor (BDNF), apoptotic and endoplasmic reticulum (ER) stress related indices were compared among the groups. UCMS alone exposed rats showed less crossings with diminished activities in open field test (OFT), increased serum corticosterone levels and enhanced expression of BDNF signaling and ER stress related markers associated with apoptosis as compared to control. In contrast, morin (60 mg/kg b.w.) cotreatment attenuated UCMS induced abnormalities as compared to UCMS alone exposed animals. The present results indicated the antidepressant-like actions of morin against UCMS in rats were partially due to its anti-apoptotic effects by regulating BDNF/Trk-B and ER stress related markers. Moreover the results of the present study indicated that the morin may act as therapeutic agent for the management of depression alone or with other currently used antidepressants.","PeriodicalId":9156,"journal":{"name":"Bioscience Biotechnology Research Communications","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44409999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Coleoptera fauna in Baghdad Campus was investigated from July 2020 to April 2021. Three habitats included inter dunal sandy area, core desert area, and area in and around the hostel which have been named as H-1, H-2 and H-3 respectively. Techniques like hand picking, plant jerking and pitfall trapping were adopted for sampling in order to explore distribution and diversity of Coleoptera fauna. 1037 samples were collected and preserved in 70% alcohol. Samples were identified on the basis of morphological characters by using bug guide. 7 families and 16 genera were sorted out. The family Tenebrionidae include the genera Blaps, Eleodes, Pimelia, Gonocephallu, and Eusattus was present. The family Coccinellidae includes genera Coccinella was also found. The family Carabidae includes Anthia, Scarites and Calosoma. The family Elateridae, Carculionidae, and Chrysomelidae contains genera Agriotes, Rhynchophorus, and Altica respectively. The Family Scarabaeidae contains the Thyce, Heteronychus, Tomarus, Phyllophagaa genera. In total count, maximum diversity and abundance of Altica was reported. Genus Anthia, Scarites, Agriotes, Rhynnchophorus, Heteronychus, and Tomarus were found in minimum but equal number. In habitat-3, maximum diversity was observed as compared to other habitats. It is concluded that these findings seemed to be helpful in ecological management of the beetles
{"title":"On the Diversity of Beetles in the Baghdad Campus, Islamia University of Bahawalpur, Pakistan","authors":"K. Nadeem","doi":"10.21786/bbrc/15.1.21","DOIUrl":"https://doi.org/10.21786/bbrc/15.1.21","url":null,"abstract":"Coleoptera fauna in Baghdad Campus was investigated from July 2020 to April 2021. Three habitats included inter dunal sandy area, core desert area, and area in and around the hostel which have been named as H-1, H-2 and H-3 respectively. Techniques like hand picking, plant jerking and pitfall trapping were adopted for sampling in order to explore distribution and diversity of Coleoptera fauna. 1037 samples were collected and preserved in 70% alcohol. Samples were identified on the basis of morphological characters by using bug guide. 7 families and 16 genera were sorted out. The family Tenebrionidae include the genera Blaps, Eleodes, Pimelia, Gonocephallu, and Eusattus was present. The family Coccinellidae includes genera Coccinella was also found. The family Carabidae includes Anthia, Scarites and Calosoma. The family Elateridae, Carculionidae, and Chrysomelidae contains genera Agriotes, Rhynchophorus, and Altica respectively. The Family Scarabaeidae contains the Thyce, Heteronychus, Tomarus, Phyllophagaa genera. In total count, maximum diversity and abundance of Altica was reported. Genus Anthia, Scarites, Agriotes, Rhynnchophorus, Heteronychus, and Tomarus were found in minimum but equal number. In habitat-3, maximum diversity was observed as compared to other habitats. It is concluded that these findings seemed to be helpful in ecological management of the beetles","PeriodicalId":9156,"journal":{"name":"Bioscience Biotechnology Research Communications","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46324399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The study was conducted in selected schools of Gurugram, Haryana. Sixty (60) primary school teachers were selected from three schools of Gurugram by using total enumeration sampling technique. Self-structured knowledge questionnaire and attitude rating scale was used to assess the knowledge and attitude of primary school teachers regarding ADHD. The findings revealed that in the pre-test, majority 41(68.3%) of teachers had inadequate knowledge and 19(31.7%) had moderate knowledge and 28(46.7%) had unfavourable attitude and 32(53.3%) had Neutral attitude whereas in the post-test, 15(25%) had moderate knowledge and majority 45(75%) had adequate knowledge and 13(21.7%) had neutral attitude and majority 47(78.3%) had favourable attitude regarding ADHD. Paired “t” test was used to observe differences between pre and post-test mean scores and were found statistically significant at 0.05 level. The study concluded that educational material like video assisted teaching had shown significant improvement in knowledge and attitude of primary school teachers.
{"title":"Effectiveness of Video-Assisted Teaching on Knowledge and Attitude Regarding Attention Deficit Hyperactivity Disorder among Primary School Teachers in Gurugram, Haryana, India: A Pre-Experimental Study","authors":"R. Deep","doi":"10.21786/bbrc/15.1.23","DOIUrl":"https://doi.org/10.21786/bbrc/15.1.23","url":null,"abstract":"The study was conducted in selected schools of Gurugram, Haryana. Sixty (60) primary school teachers were selected from three schools of Gurugram by using total enumeration sampling technique. Self-structured knowledge questionnaire and attitude rating scale was used to assess the knowledge and attitude of primary school teachers regarding ADHD. The findings revealed that in the pre-test, majority 41(68.3%) of teachers had inadequate knowledge and 19(31.7%) had moderate knowledge and 28(46.7%) had unfavourable attitude and 32(53.3%) had Neutral attitude whereas in the post-test, 15(25%) had moderate knowledge and majority 45(75%) had adequate knowledge and 13(21.7%) had neutral attitude and majority 47(78.3%) had favourable attitude regarding ADHD. Paired “t” test was used to observe differences between pre and post-test mean scores and were found statistically significant at 0.05 level. The study concluded that educational material like video assisted teaching had shown significant improvement in knowledge and attitude of primary school teachers.","PeriodicalId":9156,"journal":{"name":"Bioscience Biotechnology Research Communications","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47123237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Blackbuck, Antilope cervicapra, is a diurnal ungulate species with distinct sexual dimorphism and spellbinding beauty. Male blackbuck has mesmeric beauty with its unique darker coat, showing the increased intensity of color with age. Blackbuck is endemic to Pakistan, Nepal, India and Bangladesh but now its population is reduced to a few areas. Threats such as hunting, stress, habitat loss, diseases, poaching, road accidents, habitat fragmentation, interspecific competition, predation pressure etc., have reduced the population size of blackbuck to a threatened level. Therefore, different conservation strategies are underway to increment its count for improvement of faunal diversity, tourism development and dispersal of the local culture in South Asia. Captive breeding of species is the most efficacious conservation strategy in South Asia so far. Furthermore, various rules and regulations along with strategies like hormone-mediated conservation by injections of prostaglandin and artificial insemination are assisting the species by increasing its birth rate. Genetic studies, introduction to non-endemic but suitable habitat and religious affiliation of communities also contributed to blackbuck conservation. Current conservation practices are helping to conserve the blackbuck but are associated with a few concerns also, thereby proper management, planning, monitoring of conservation practices is required. Population size, distribution range, threats confronted by species, conservation practi
{"title":"Population Status, Distribution, Threats and Conservation of the Blackbuck, Antilope cervicapra in South Asia: An Updated Review","authors":"Rabia Tahir","doi":"10.21786/bbrc/15.1.4","DOIUrl":"https://doi.org/10.21786/bbrc/15.1.4","url":null,"abstract":"Blackbuck, Antilope cervicapra, is a diurnal ungulate species with distinct sexual dimorphism and spellbinding beauty. Male blackbuck has mesmeric beauty with its unique darker coat, showing the increased intensity of color with age. Blackbuck is endemic to Pakistan, Nepal, India and Bangladesh but now its population is reduced to a few areas. Threats such as hunting, stress, habitat loss, diseases, poaching, road accidents, habitat fragmentation, interspecific competition, predation pressure etc., have reduced the population size of blackbuck to a threatened level. Therefore, different conservation strategies are underway to increment its count for improvement of faunal diversity, tourism development and dispersal of the local culture in South Asia. Captive breeding of species is the most efficacious conservation strategy in South Asia so far. Furthermore, various rules and regulations along with strategies like hormone-mediated conservation by injections of prostaglandin and artificial insemination are assisting the species by increasing its birth rate. Genetic studies, introduction to non-endemic but suitable habitat and religious affiliation of communities also contributed to blackbuck conservation. Current conservation practices are helping to conserve the blackbuck but are associated with a few concerns also, thereby proper management, planning, monitoring of conservation practices is required. Population size, distribution range, threats confronted by species, conservation practi","PeriodicalId":9156,"journal":{"name":"Bioscience Biotechnology Research Communications","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44102134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Text neck syndrome is a stress/overuse injury in the neck caused by leaning forward for extended periods of time, such as when reading and texting on a cellphone, and has been linked to stress injuries. The primary cause of the disorder is the weight of the head which while bending it forward or looking down at a 45 to 60 degree angle during texting produces around 50 to 60 pounds of stress on the neck, increasing pressure over time and causing damage. While the majority of treatment is mostly based on. The aim of the present study was to evaluate the effectiveness of treatment between osteopathic intervention and modified cervical exercise therapy program among text neck syndrome patients. In total, seventy two patients were selected based on inclusion/exclusion criteria out of which 6 dropped out. In the end, 66 patients were randomly were allocated into two groups namely, the osteopathic treatment group(n=33) and modified cervical exercise group(n=33). The treatment program was followed for 3 weeks and the patients were evaluated for Numeric Pain Rating Scale(NPRS), Neck disability index(NDI) , Cervical range of motion as well as cranio-vertebral angle to measure the treatment effectiveness. Paired t-test showed significant differences in both groups between variables mean scores from the baseline readings after three weeks of intervention. Statistical significant differences were observed in the mean scores for all the outcome measures including NPRS, Cervical ROM, Craniovertebral angle and NDI. The result of the study suggests that Osteopathic treatment technique along with modified cervical exercise therapy is more effective than modified cervical exercise alone in treating text neck syndrome patients(p<0.05).
{"title":"Effectiveness of Osteopathic Treatment Versus Modified Cervical Exercise Therapy in Patients with Text Neck Syndrome","authors":"M. Miraj","doi":"10.21786/bbrc/15.1.41","DOIUrl":"https://doi.org/10.21786/bbrc/15.1.41","url":null,"abstract":"Text neck syndrome is a stress/overuse injury in the neck caused by leaning forward for extended periods of time, such as when reading and texting on a cellphone, and has been linked to stress injuries. The primary cause of the disorder is the weight of the head which while bending it forward or looking down at a 45 to 60 degree angle during texting produces around 50 to 60 pounds of stress on the neck, increasing pressure over time and causing damage. While the majority of treatment is mostly based on. The aim of the present study was to evaluate the effectiveness of treatment between osteopathic intervention and modified cervical exercise therapy program among text neck syndrome patients. In total, seventy two patients were selected based on inclusion/exclusion criteria out of which 6 dropped out. In the end, 66 patients were randomly were allocated into two groups namely, the osteopathic treatment group(n=33) and modified cervical exercise group(n=33). The treatment program was followed for 3 weeks and the patients were evaluated for Numeric Pain Rating Scale(NPRS), Neck disability index(NDI) , Cervical range of motion as well as cranio-vertebral angle to measure the treatment effectiveness. Paired t-test showed significant differences in both groups between variables mean scores from the baseline readings after three weeks of intervention. Statistical significant differences were observed in the mean scores for all the outcome measures including NPRS, Cervical ROM, Craniovertebral angle and NDI. The result of the study suggests that Osteopathic treatment technique along with modified cervical exercise therapy is more effective than modified cervical exercise alone in treating text neck syndrome patients(p<0.05).","PeriodicalId":9156,"journal":{"name":"Bioscience Biotechnology Research Communications","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43487455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cancer has become one of the most common clinical reasons of death all over the world, with more than nine million deaths each year. Throughout the history, many traditional plants have been used to treat various types of cancers. The scientifically researched anticancer plants, with over 3000 species are novel candidates for anticancer drugs, because more than 80% of society believes in traditional medicine as its cure. The aim of this study was to determine the effects of Costus speciosus root extract on human lung cancer (LC) cells (A549). Treatment of (A549) cells with ethyl acetate Costus root extract significantly lowered the cell viability. IC50 was calculated, after 24, 48 and72 h, with concentration of 25, 40, 70 and 100 μg/100μl. The MTT assay was used to test the viability of the cells. The effect of (EACR) on cell adhesion and migration was demonstrated in a wound healing assay, mitochondrial membrane potential was detected in a JC-1 assay, and DNA damage and repair were demonstrated in a comet assay. There was a noticeable reduction in (LC) development. Experiments underlying the anti-cancer activity of (EACR) on (A549) cells may be regarded as one of the most promising targets for novel cancer treatment strategies (LC).
{"title":"The Effects of Costus speciosus Root Extract on Cultured Human Lung Cancer Cells, A549","authors":"A. Omar","doi":"10.21786/bbrc/15.1.25","DOIUrl":"https://doi.org/10.21786/bbrc/15.1.25","url":null,"abstract":"Cancer has become one of the most common clinical reasons of death all over the world, with more than nine million deaths each year. Throughout the history, many traditional plants have been used to treat various types of cancers. The scientifically researched anticancer plants, with over 3000 species are novel candidates for anticancer drugs, because more than 80% of society believes in traditional medicine as its cure. The aim of this study was to determine the effects of Costus speciosus root extract on human lung cancer (LC) cells (A549). Treatment of (A549) cells with ethyl acetate Costus root extract significantly lowered the cell viability. IC50 was calculated, after 24, 48 and72 h, with concentration of 25, 40, 70 and 100 μg/100μl. The MTT assay was used to test the viability of the cells. The effect of (EACR) on cell adhesion and migration was demonstrated in a wound healing assay, mitochondrial membrane potential was detected in a JC-1 assay, and DNA damage and repair were demonstrated in a comet assay. There was a noticeable reduction in (LC) development. Experiments underlying the anti-cancer activity of (EACR) on (A549) cells may be regarded as one of the most promising targets for novel cancer treatment strategies (LC).","PeriodicalId":9156,"journal":{"name":"Bioscience Biotechnology Research Communications","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43558898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Amongst the cytoskeletal proteins of bacteria, MreB is known to have very crucial role in modulating shape of the bacteria. Present study involves the use of biocide (A-22) which minimizes the bacterial size augmenting with minimal antibiotic consumption. Intended experiment is designed to be carried out on selected pure strains of gram-positive and gram-negative bacteria namely Lactobacillus rhamnosous ATCC 7469 and Pseudomonas aeruginosa ATCC 27853 respectively. The pure strains are exposed to biocide and changes in the shape is recorded by means of Foldscope (Origami based paper microscope, Prakash Labs) and in-vivo assessment done using antibiotic sensitivity assays with different antibiotics.The novel biocide specifically targeting bacterial cytoskeletal protein, that determines rod shape among bacterial population. The said compound is also experimented as combinational drug along with conventional antibiotics to reduce antibiotic dose needed to kill and to overcome antibiotic resistance. The A-22has reduced nearly 60-70% antibiotic usage. In Pseudomonas aeruginosa ATCC 27853 when tested for MIC using A-22 and different antibiotics, it was found that 0.5 µg/ml of ampicillin, 1 µg/ml of streptomycin and 5 µg/ml erythromycin were effective in curtailing bacteria against conventional antibiotic concentrations ampicillin 128 µg/ml streptomycin 32 µg/ml, erythromycin 64 µg/ml. Compared to doses of antibiotics required to kill bacteria, the combinational drug of biocide and antibiotic have shown promising effects in killing bacteria at very less concentration, this can useful for treating most diseases caused by antimicrobial resistance bacterial populations.
{"title":"Biological and Computational Approach to Modify Bacterial Size and Reduce its Antibiotic Consumption Targeting Mreb Bacterial Cytoskeletal Protein","authors":"P. J.","doi":"10.21786/bbrc/15.1.11","DOIUrl":"https://doi.org/10.21786/bbrc/15.1.11","url":null,"abstract":"Amongst the cytoskeletal proteins of bacteria, MreB is known to have very crucial role in modulating shape of the bacteria. Present study involves the use of biocide (A-22) which minimizes the bacterial size augmenting with minimal antibiotic consumption. Intended experiment is designed to be carried out on selected pure strains of gram-positive and gram-negative bacteria namely Lactobacillus rhamnosous ATCC 7469 and Pseudomonas aeruginosa ATCC 27853 respectively. The pure strains are exposed to biocide and changes in the shape is recorded by means of Foldscope (Origami based paper microscope, Prakash Labs) and in-vivo assessment done using antibiotic sensitivity assays with different antibiotics.The novel biocide specifically targeting bacterial cytoskeletal protein, that determines rod shape among bacterial population. The said compound is also experimented as combinational drug along with conventional antibiotics to reduce antibiotic dose needed to kill and to overcome antibiotic resistance. The A-22has reduced nearly 60-70% antibiotic usage. In Pseudomonas aeruginosa ATCC 27853 when tested for MIC using A-22 and different antibiotics, it was found that 0.5 µg/ml of ampicillin, 1 µg/ml of streptomycin and 5 µg/ml erythromycin were effective in curtailing bacteria against conventional antibiotic concentrations ampicillin 128 µg/ml streptomycin 32 µg/ml, erythromycin 64 µg/ml. Compared to doses of antibiotics required to kill bacteria, the combinational drug of biocide and antibiotic have shown promising effects in killing bacteria at very less concentration, this can useful for treating most diseases caused by antimicrobial resistance bacterial populations.","PeriodicalId":9156,"journal":{"name":"Bioscience Biotechnology Research Communications","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44681779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：