The aim of the present study was to establish age-dependent histological changes occurring in the bursa of Fabricius of bronze turkeys. To this end, histological preparations from 60 clinically healthy birds (30 males and 30 females) were examined by light microscopy. The turkeys were divided into ten age groups – at 1, 7, 14, 28, 35, 49, 56, 90, 120 and 240 days of age. Specimens were processed by routine histological techniques. At one day of age, the wall of the bursa of Fab-ricius comprised 4 tissue layers: tunica mucosa, tela submucosa, tunica muscularis and tunica serosa, and the mucosa formed high fold directed to the lumen. These folds were filled with lym-phoid follicles, whose cortex and medulla were indistinguishable. From the 7th to the 120th days if age, the size of follicles increased and the peripherally located dark-staining cortex was clearly distinct from the centrally located lighter staining medulla. On the 240th day, signs of bursa of Fabricius’ involution were visible – destruction of follicles, cystic formations, thickening of inter-follicular connective tissue septa and fat accumulation within the wall. The obtained results al-lowed concluding that the physiological involution of the bursa of Fabricius started at the onset of sexual maturity.
{"title":"Age-related histology of the bursa of Fabricius in bronze turkeys (Meleagris meleagris gallopavo)","authors":"G. Penchev","doi":"10.15547/bjvm.2387","DOIUrl":"https://doi.org/10.15547/bjvm.2387","url":null,"abstract":"The aim of the present study was to establish age-dependent histological changes occurring in the bursa of Fabricius of bronze turkeys. To this end, histological preparations from 60 clinically healthy birds (30 males and 30 females) were examined by light microscopy. The turkeys were divided into ten age groups – at 1, 7, 14, 28, 35, 49, 56, 90, 120 and 240 days of age. Specimens were processed by routine histological techniques. At one day of age, the wall of the bursa of Fab-ricius comprised 4 tissue layers: tunica mucosa, tela submucosa, tunica muscularis and tunica serosa, and the mucosa formed high fold directed to the lumen. These folds were filled with lym-phoid follicles, whose cortex and medulla were indistinguishable. From the 7th to the 120th days if age, the size of follicles increased and the peripherally located dark-staining cortex was clearly distinct from the centrally located lighter staining medulla. On the 240th day, signs of bursa of Fabricius’ involution were visible – destruction of follicles, cystic formations, thickening of inter-follicular connective tissue septa and fat accumulation within the wall. The obtained results al-lowed concluding that the physiological involution of the bursa of Fabricius started at the onset of sexual maturity.","PeriodicalId":9279,"journal":{"name":"BULGARIAN JOURNAL OF VETERINARY MEDICINE","volume":"126 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79182201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ahmed Majeed Al-Shammari, M. A. Al-Mudhafr, E. D. Chalap Al- Grawi, Z. Al-Hili, N. Yaseen
Cancer cells heavily utilise angiogenesis process to increase vascularisation for tumour mass growth and spread, so targeting this process is important to create an effective therapy. The AMHA1 strain of Newcastle disease virus (NDV) is an RNA virus with natural oncotropism. NDV induces direct tumour cytolysis, apoptosis, and immune stimulation. This work aimed to test NDV anti-angiogenic activity in a breast cancer model. To evaluate NDV’s antitumour effect in vivo, NDV was tested against mammary adenocarcinoma AN3 transplanted in syngeneic immunocompetent mice. In vivo antiangiogenic activity was evaluated by quantifying the blood vessels in treated and control tumour sections. In vitro experiments that exposed AMN3 mammary adenocarcinoma cells and Hep-2 laryngeal carcinoma cells to NDV at different time intervals were performed to identify the exact mechanism of anti-angiogenesis by using angiogenesis microarray slides. In vivo results showed significant tumour regression and significant decrease in blood vessel formation in treated tumour sections. The in vitro microarray analysis of 14 different angiogenesis factors revealed that NDV downregulated angiopoietin-1, angiopoietin-2, and epidermal growth factor in mammary adenocarcinoma cells. However, NDV elicited a different effect on Hep-2 as represented by the downregulation of inducible protein 10, intracellular adhesion molecule-1, and basic fibroblast growth factor beta in NDV-infected tumour cells. It was found out that microarray analysis results helped interpret the in vivo data. The results suggested that the NDV oncolytic strain reduced angiogenesis by interfering with angiogenesis factors that might reduce tumour cell proliferation, infiltration, and invasion.
{"title":"Newcastle disease virus suppresses angiogenesis in mammary adenocarcinoma models","authors":"Ahmed Majeed Al-Shammari, M. A. Al-Mudhafr, E. D. Chalap Al- Grawi, Z. Al-Hili, N. Yaseen","doi":"10.15547/bjvm.2020-0019","DOIUrl":"https://doi.org/10.15547/bjvm.2020-0019","url":null,"abstract":"Cancer cells heavily utilise angiogenesis process to increase vascularisation for tumour mass growth and spread, so targeting this process is important to create an effective therapy. The AMHA1 strain of Newcastle disease virus (NDV) is an RNA virus with natural oncotropism. NDV induces direct tumour cytolysis, apoptosis, and immune stimulation. This work aimed to test NDV anti-angiogenic activity in a breast cancer model. To evaluate NDV’s antitumour effect in vivo, NDV was tested against mammary adenocarcinoma AN3 transplanted in syngeneic immunocompetent mice. In vivo antiangiogenic activity was evaluated by quantifying the blood vessels in treated and control tumour sections. In vitro experiments that exposed AMN3 mammary adenocarcinoma cells and Hep-2 laryngeal carcinoma cells to NDV at different time intervals were performed to identify the exact mechanism of anti-angiogenesis by using angiogenesis microarray slides. In vivo results showed significant tumour regression and significant decrease in blood vessel formation in treated tumour sections. The in vitro microarray analysis of 14 different angiogenesis factors revealed that NDV downregulated angiopoietin-1, angiopoietin-2, and epidermal growth factor in mammary adenocarcinoma cells. However, NDV elicited a different effect on Hep-2 as represented by the downregulation of inducible protein 10, intracellular adhesion molecule-1, and basic fibroblast growth factor beta in NDV-infected tumour cells. It was found out that microarray analysis results helped interpret the in vivo data. The results suggested that the NDV oncolytic strain reduced angiogenesis by interfering with angiogenesis factors that might reduce tumour cell proliferation, infiltration, and invasion.","PeriodicalId":9279,"journal":{"name":"BULGARIAN JOURNAL OF VETERINARY MEDICINE","volume":"41 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75657618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Saranjam, M. Farhoodi Moghaddam, G. Akbari, M. Mohammadsadegh, N. Farzaneh
The aims of the present study were to evaluate the effects of different dry period (DP) lengths on milk fat to protein ratio (FPR) and metabolic status – blood leptin, adiponectin and non-esterified fatty acids (NEFA) concentrations in dairy cows, and their associations with the result of the first timed artificial insemination (TAI). Cows were blocked either to short DP (SDP; 30±2 days; n=72) or conventional DP (CDP; 60±2 days; n=76). Milk FPR was calculated at 30 and 60 days in milk (DIM). Body condition score (BCS) was recorded at –60, –30, calving, and 60 DIM. Blood samples were obtained at –60, –30, –7, calving, +7, +30, and +60 DIM for serum metabolites measurement. TAI was implemented between 65–75 DIM for all cows. Milk FPR and its changes were statistically analysed using an independent sample t test. To assess the impact of time, the pattern of BCS, and serum metabolites on the result of the first AI, repeated measure ANOVA was used. Only FPR-30 DIM revealed significant difference between pregnant and non-pregnant cows in SDP group (P<0.01). Reduced BCS loss was observed in the SDP group and followed by slightly higher probability of pregnancy at first AI (P=0.19). Leptin was not altered by shortening the DP (P≥0.1). Significant differences were observed in blood adiponectin prepartum (P<0.001) and at +7 DIM (P<0.01), as well as in NEFA at +7 and +30 DIM between the two groups (P<0.05). Pregnant cows following the first AI had significantly high postpartum leptin concentrations (P<0.05), high prepartum adiponectin (P≤0.001), and lower NEFA at +7 DIM (P<0.01) in the SDP group. In conclusion, shortening the dry period caused reduced BCS loss postpartum and variations in serum metabolits that favoured the possibility of pregnancy at first AI.
{"title":"Effect of different dry period duration on milk components and serum metabolites, and their associations with the first conception rate in multiparous Holstein dairy cows","authors":"N. Saranjam, M. Farhoodi Moghaddam, G. Akbari, M. Mohammadsadegh, N. Farzaneh","doi":"10.15547/bjvm.2020-0123","DOIUrl":"https://doi.org/10.15547/bjvm.2020-0123","url":null,"abstract":"The aims of the present study were to evaluate the effects of different dry period (DP) lengths on milk fat to protein ratio (FPR) and metabolic status – blood leptin, adiponectin and non-esterified fatty acids (NEFA) concentrations in dairy cows, and their associations with the result of the first timed artificial insemination (TAI). Cows were blocked either to short DP (SDP; 30±2 days; n=72) or conventional DP (CDP; 60±2 days; n=76). Milk FPR was calculated at 30 and 60 days in milk (DIM). Body condition score (BCS) was recorded at –60, –30, calving, and 60 DIM. Blood samples were obtained at –60, –30, –7, calving, +7, +30, and +60 DIM for serum metabolites measurement. TAI was implemented between 65–75 DIM for all cows. Milk FPR and its changes were statistically analysed using an independent sample t test. To assess the impact of time, the pattern of BCS, and serum metabolites on the result of the first AI, repeated measure ANOVA was used. Only FPR-30 DIM revealed significant difference between pregnant and non-pregnant cows in SDP group (P<0.01). Reduced BCS loss was observed in the SDP group and followed by slightly higher probability of pregnancy at first AI (P=0.19). Leptin was not altered by shortening the DP (P≥0.1). Significant differences were observed in blood adiponectin prepartum (P<0.001) and at +7 DIM (P<0.01), as well as in NEFA at +7 and +30 DIM between the two groups (P<0.05). Pregnant cows following the first AI had significantly high postpartum leptin concentrations (P<0.05), high prepartum adiponectin (P≤0.001), and lower NEFA at +7 DIM (P<0.01) in the SDP group. In conclusion, shortening the dry period caused reduced BCS loss postpartum and variations in serum metabolits that favoured the possibility of pregnancy at first AI.","PeriodicalId":9279,"journal":{"name":"BULGARIAN JOURNAL OF VETERINARY MEDICINE","volume":"129 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83024971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Milcheva, P. Janega, S. Petkova, K. S. Todorova, D. Ivanov, P. Babál
This study was aimed to describe some glycosylation changes in the Nurse cell of Trichinella spiralis in mouse skeletal muscle. Tissue specimens were subjected to lectin histochemistry with Maackia amurensis lectin (MAL), Peanut agglutinin (PNA) and neuraminidase desialylation in order to verify and analyse the structure of α-2,3-sialylated glycoproteins, discovered within the affected sarcoplasm. The expressions of two sialyltransferases were examined by immunohistochemistry. It was found out that the occupied portion of skeletal muscle cell responded with synthesis of presumable sialyl-T-antigen and α-2,3-sialyllactosamine structure, that remained accumulated during the time course of Nurse cell development. The enzymes β-galactoside-α-2,3-sialyltransferases 2 and 4, which could be responsible for the sialylation of each of these structures, were however not present in the invaded muscle portions, although their expressions in the healthy surrounding tissue remained persistent. Our results contribute to the progressive understanding about the amazing abilities of Trichinella spiralis to manipulate the genetic programme of its host.
{"title":"Absence of ST3Gal2 and ST3Gal4 sialyltransferase expressions in the nurse cell of Trichinella spiralis","authors":"R. Milcheva, P. Janega, S. Petkova, K. S. Todorova, D. Ivanov, P. Babál","doi":"10.15547/bjvm.2020-0006","DOIUrl":"https://doi.org/10.15547/bjvm.2020-0006","url":null,"abstract":"This study was aimed to describe some glycosylation changes in the Nurse cell of Trichinella spiralis in mouse skeletal muscle. Tissue specimens were subjected to lectin histochemistry with Maackia amurensis lectin (MAL), Peanut agglutinin (PNA) and neuraminidase desialylation in order to verify and analyse the structure of α-2,3-sialylated glycoproteins, discovered within the affected sarcoplasm. The expressions of two sialyltransferases were examined by immunohistochemistry. It was found out that the occupied portion of skeletal muscle cell responded with synthesis of presumable sialyl-T-antigen and α-2,3-sialyllactosamine structure, that remained accumulated during the time course of Nurse cell development. The enzymes β-galactoside-α-2,3-sialyltransferases 2 and 4, which could be responsible for the sialylation of each of these structures, were however not present in the invaded muscle portions, although their expressions in the healthy surrounding tissue remained persistent. Our results contribute to the progressive understanding about the amazing abilities of Trichinella spiralis to manipulate the genetic programme of its host.","PeriodicalId":9279,"journal":{"name":"BULGARIAN JOURNAL OF VETERINARY MEDICINE","volume":"47 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91218303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Karamian, A. Soleimanzadeh, A. Tukmechi, R. Batavani
Bovine tuberculosis is an important global zoonosis. The causative agent of the disease is Mycobacterium bovis, belonging to the Mycobacterium tuberculosis complex. This study aimed to identify vertical transmission of Mycobacterium bovis in tuberculin positive pregnant dairy cows referred to the slaughterhouse and commercial sperm used to inseminate them in Gonbad, Gorgan province, Iran, by PCR assay. From March to September 2016, one thousand and seven hundred artificially inseminated cows were subjected to the tuberculin test; then, tuberculin positive cows were referred to the Gonbad slaughterhouse. Samples containing milk, lymph node, placenta, foetal abomasal fluid, and amniotic fluid were prepared from all affected cows and stored at -80 °C until analysis. Bacterial DNA was extracted from all specimens by a commercial kit. Detection was performed by amplifying pncATB gene using special primers via the polymerase chain reaction method. Results of tuberculin test revealed 144 positive cases (8.47%). PCR results for 48 milk samples, 120 biopsy samples, 63 samples of foetuses showed 9, 93 and 43 positive cases, respectively which all belonged to tuberculin positive samples. No positive sperm samples were identified. It was concluded that the infection rate with M. bovis in Montbéliarde cattle was high and also, that vertical transmission was not seen.
{"title":"PCR investigation of the vertical transmission of Mycobacterium bovis in Montbéliarde cattle in Gonbad, northeast of Iran","authors":"M. Karamian, A. Soleimanzadeh, A. Tukmechi, R. Batavani","doi":"10.15547/bjvm.2020-0135","DOIUrl":"https://doi.org/10.15547/bjvm.2020-0135","url":null,"abstract":"Bovine tuberculosis is an important global zoonosis. The causative agent of the disease is Mycobacterium bovis, belonging to the Mycobacterium tuberculosis complex. This study aimed to identify vertical transmission of Mycobacterium bovis in tuberculin positive pregnant dairy cows referred to the slaughterhouse and commercial sperm used to inseminate them in Gonbad, Gorgan province, Iran, by PCR assay. From March to September 2016, one thousand and seven hundred artificially inseminated cows were subjected to the tuberculin test; then, tuberculin positive cows were referred to the Gonbad slaughterhouse. Samples containing milk, lymph node, placenta, foetal abomasal fluid, and amniotic fluid were prepared from all affected cows and stored at -80 °C until analysis. Bacterial DNA was extracted from all specimens by a commercial kit. Detection was performed by amplifying pncATB gene using special primers via the polymerase chain reaction method. Results of tuberculin test revealed 144 positive cases (8.47%). PCR results for 48 milk samples, 120 biopsy samples, 63 samples of foetuses showed 9, 93 and 43 positive cases, respectively which all belonged to tuberculin positive samples. No positive sperm samples were identified. It was concluded that the infection rate with M. bovis in Montbéliarde cattle was high and also, that vertical transmission was not seen.","PeriodicalId":9279,"journal":{"name":"BULGARIAN JOURNAL OF VETERINARY MEDICINE","volume":"101 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74553243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
I. Fasulkov, M. Karadaev, D. Zapryanova, T. Mircheva, N. Vasilev, F. Ceciliani
The goal of this study was to determine the plasma concentrations of the acute phase proteins fibrinogen and ceruloplasmin during the pregnancy and postpartum period in goats. The experiment was carried out with six clinically healthy Bulgarian native goats, aged 4-6 years, weighing 49-56 kg, reared at the Biobase of the Faculty of Veterinary Medicine – Stara Zagora. Blood samples for assay of plasma concentrations of the studied acute-phase proteins during pregnancy were collected over 7-day intervals, from the 14th to the 147th day of pregnancy. During the postpartum period, samples were obtained at 3-day intervals between the 1st and 15th postpartum day, as well as once on the 20th and 30th day after the parturition. The results indicated that throughout the first trimester of the pregnancy, the highest average plasma fibrinogen values were established on the 14th day, while significantly lower values (P<0.05) were measured on the 21st day. At this period, the values of plasma ceruloplasmin were the lowest on the 28th day and significantly higher (P<0.01) at the end of the first pregnancy trimester. During the second trimester of the pregnancy there was a clear tendency towards a decrease in the levels of the examined acute phase proteins. At the last pregnancy trimester, an increase in the levels of both acute phase proteins was observed. During the postpartum period there was a significant decrease (P<0.05) in the levels of ceruloplasmin and fibrinogen on the 20th day, compared to the first postpartum day. The established average values of the examined acute phase proteins could be used for comparison purposes in cases of pathological conditions during the pregnancy and post partum period of goats.
{"title":"Comparative study on plasma fibrinogen and ceruloplasmin concentrations during pregnancy and postpartum period in Bulgarian native goats","authors":"I. Fasulkov, M. Karadaev, D. Zapryanova, T. Mircheva, N. Vasilev, F. Ceciliani","doi":"10.15547/bjvm.2372","DOIUrl":"https://doi.org/10.15547/bjvm.2372","url":null,"abstract":"The goal of this study was to determine the plasma concentrations of the acute phase proteins fibrinogen and ceruloplasmin during the pregnancy and postpartum period in goats. The experiment was carried out with six clinically healthy Bulgarian native goats, aged 4-6 years, weighing 49-56 kg, reared at the Biobase of the Faculty of Veterinary Medicine – Stara Zagora. Blood samples for assay of plasma concentrations of the studied acute-phase proteins during pregnancy were collected over 7-day intervals, from the 14th to the 147th day of pregnancy. During the postpartum period, samples were obtained at 3-day intervals between the 1st and 15th postpartum day, as well as once on the 20th and 30th day after the parturition. The results indicated that throughout the first trimester of the pregnancy, the highest average plasma fibrinogen values were established on the 14th day, while significantly lower values (P<0.05) were measured on the 21st day. At this period, the values of plasma ceruloplasmin were the lowest on the 28th day and significantly higher (P<0.01) at the end of the first pregnancy trimester. During the second trimester of the pregnancy there was a clear tendency towards a decrease in the levels of the examined acute phase proteins. At the last pregnancy trimester, an increase in the levels of both acute phase proteins was observed. During the postpartum period there was a significant decrease (P<0.05) in the levels of ceruloplasmin and fibrinogen on the 20th day, compared to the first postpartum day. The established average values of the examined acute phase proteins could be used for comparison purposes in cases of pathological conditions during the pregnancy and post partum period of goats.","PeriodicalId":9279,"journal":{"name":"BULGARIAN JOURNAL OF VETERINARY MEDICINE","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83721216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The aim of the study was to evaluate the potential of survival and growth dynamics of Listeria monocytogenes strains and Salmonella enterica subsp. enterica serovar Enteritidis and Typhimurium, inoculated artificially (individually and in mixture) on two ready-to-eat (RTE) cooked smoked meat products. For the purpose of the study 120 slices of cooked smoked sausage and 40 slices of cooked smoked loin were purchased and inoculated with the strains. Two storage temperatures were selected: 6 oC and 10 oC for 8 days. The study was performed as challenge tests in a secondary contamination scenario to investigate the presence and/or absence of pathogenic bacteria during the shelf life of the products. The inoculum levels at the start of the experiment were 4.46 log10 CFU/g and 2.88 log10 CFU/g for the L. monocytogenes strains at the surface of the cooked smoked loin and cooked smoked sausage respectively. Using the same inoculation method, but adding Salmonella enterica serovars to the mixture, the inoculum levels were 4.15 log10 CFU/g at the surface of the cooked smoked loin and 2.94 log10 CFU/g at the surface of the cooked smoked sausage. L. monocytogenes was detected at all sampling days on both storage temperatures. It showed an average increase by 0.5–1.0 log10 CFU/g on the cooked smoked sausage and by 2.0–3.3 log10 CFU/g for the cooked smoked pork loin for the duration of the study. Salmonella enterica serovars were also present at each sampling day on both storage temperatures. Typical colonies were isolated and serotyped, confirming the survival of these pathogenic bacteria. Salmonella enterica subsp. enterica serovar Typhimurium was the predominant serovar at almost every sampling day. The results from our study showed no competitive relationship in the presence of Salmonella and L. monocytogenes in contaminated meat products. The two types of microorganisms were successfully adapted and developed independently under appropriate conditions, including temperature, humidity, water activity and pH.
{"title":"Survival and growth dynamics of Listeria monocytogenes and Salmonella spp. on artificially contaminated cooked ready-to-eat meat products","authors":"M. Milanov, G. Mateva, T. Stoyanchev","doi":"10.15547/bjvm.2430","DOIUrl":"https://doi.org/10.15547/bjvm.2430","url":null,"abstract":"The aim of the study was to evaluate the potential of survival and growth dynamics of Listeria monocytogenes strains and Salmonella enterica subsp. enterica serovar Enteritidis and Typhimurium, inoculated artificially (individually and in mixture) on two ready-to-eat (RTE) cooked smoked meat products. For the purpose of the study 120 slices of cooked smoked sausage and 40 slices of cooked smoked loin were purchased and inoculated with the strains. Two storage temperatures were selected: 6 oC and 10 oC for 8 days. The study was performed as challenge tests in a secondary contamination scenario to investigate the presence and/or absence of pathogenic bacteria during the shelf life of the products. The inoculum levels at the start of the experiment were 4.46 log10 CFU/g and 2.88 log10 CFU/g for the L. monocytogenes strains at the surface of the cooked smoked loin and cooked smoked sausage respectively. Using the same inoculation method, but adding Salmonella enterica serovars to the mixture, the inoculum levels were 4.15 log10 CFU/g at the surface of the cooked smoked loin and 2.94 log10 CFU/g at the surface of the cooked smoked sausage. L. monocytogenes was detected at all sampling days on both storage temperatures. It showed an average increase by 0.5–1.0 log10 CFU/g on the cooked smoked sausage and by 2.0–3.3 log10 CFU/g for the cooked smoked pork loin for the duration of the study. Salmonella enterica serovars were also present at each sampling day on both storage temperatures. Typical colonies were isolated and serotyped, confirming the survival of these pathogenic bacteria. Salmonella enterica subsp. enterica serovar Typhimurium was the predominant serovar at almost every sampling day. The results from our study showed no competitive relationship in the presence of Salmonella and L. monocytogenes in contaminated meat products. The two types of microorganisms were successfully adapted and developed independently under appropriate conditions, including temperature, humidity, water activity and pH.","PeriodicalId":9279,"journal":{"name":"BULGARIAN JOURNAL OF VETERINARY MEDICINE","volume":"69 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80929401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Khovand, S. R. Nourollahi Fard, M. Khalili, M. Jajarmi, H. S. Hormozzaie
Ehrlichia is an etiologic agent of ehrlichiosis in humans and some animals. Rhipicephalus sanguineus is the main vector of the Ehrlichia canis and dogs, red foxes and yellow jackals are reservoirs of the bacterium. This tick has a worldwide distribution and is regarded as one of the commonest species of ticks in Iran. This research aimed to detect Ehrlichia spp. in R. sanguineus isolated from stray dogs in Central and Southeast Iran (Isfahan and Zabol), by using polymerase chain reaction (PCR) and to evaluate the prevalence of the microorganism in these two areas. Tick samples were collected from stray dogs in Isfahan and Zabol between April and June of 2018. The DNA extraction was performed with commercial kits. PCR was done to determine the 336 bp fragment related to Ehrlichia spp. Overall, 15.21% of pools in both areas were positive for Ehrlichia, 21.42% and 10% of pools were from Isfahan and Zabol respectively. The results confirmed the presence of Ehrlichia spp. in R. sanguineus in stray dogs revealing that dogs and their ticks may have a significant role in the epidemiology of the disease.
{"title":"Detection of Ehrlichia spp. in ticks collected from stray dogs in Central and Southeastern Iran","authors":"H. Khovand, S. R. Nourollahi Fard, M. Khalili, M. Jajarmi, H. S. Hormozzaie","doi":"10.15547/bjvm.2020-0128","DOIUrl":"https://doi.org/10.15547/bjvm.2020-0128","url":null,"abstract":"Ehrlichia is an etiologic agent of ehrlichiosis in humans and some animals. Rhipicephalus sanguineus is the main vector of the Ehrlichia canis and dogs, red foxes and yellow jackals are reservoirs of the bacterium. This tick has a worldwide distribution and is regarded as one of the commonest species of ticks in Iran. This research aimed to detect Ehrlichia spp. in R. sanguineus isolated from stray dogs in Central and Southeast Iran (Isfahan and Zabol), by using polymerase chain reaction (PCR) and to evaluate the prevalence of the microorganism in these two areas. Tick samples were collected from stray dogs in Isfahan and Zabol between April and June of 2018. The DNA extraction was performed with commercial kits. PCR was done to determine the 336 bp fragment related to Ehrlichia spp. Overall, 15.21% of pools in both areas were positive for Ehrlichia, 21.42% and 10% of pools were from Isfahan and Zabol respectively. The results confirmed the presence of Ehrlichia spp. in R. sanguineus in stray dogs revealing that dogs and their ticks may have a significant role in the epidemiology of the disease.","PeriodicalId":9279,"journal":{"name":"BULGARIAN JOURNAL OF VETERINARY MEDICINE","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87061318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K. Todorova, A. Georgieva, R. Milcheva, D. Ivanov, I. Kalkanov
Trichothecenes are mycotoxins that occur in grains and can lead to acute and chronic poisoning in animals and humans. Deoxynivalenol (DON) is a type B trichothecene affecting protein synthesis, immune system, leading to brain, blood and kidney disorders. The aim of this work was to evaluate in vitro the cytotoxicity and the pathological effects of DON in short-term experiments on cells from non-tumour and tumour permanent cell lines and to compare their sensitivity. Cell cultivation of BALB/c 3T3, DEC 99, MDA-MB-231, MCF-7 and Hela cells was performed. Quantitative and qualitative methods evaluating cytotoxicity on the base of statistical and morphological analyses for determining the impact on the viability and proliferative activity were used: Neutral Red Uptake (NRU) cytotoxicity test, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test and fluorescence microscopy. The cytotoxic effect of DON was assessed after an exposure period of 24 h. DON treatment induced significant alterations in the growth and morphology of the cells, involving early and late apoptosis and necrosis signs. Statistically significant decrease of the viability of all cell lines was established at concentrations of DON starting from 1.9 µg/mL to 3.7 µg/mL, the mean IC50 concentrations were calculated. According to the IC50 values the hierarchical order of cell lines’ sensitivity was determined.
{"title":"Cytotoxicity of the Fusarium mycotoxin deoxynivalenol on mammalian and avian cell lines","authors":"K. Todorova, A. Georgieva, R. Milcheva, D. Ivanov, I. Kalkanov","doi":"10.15547/bjvm.2331","DOIUrl":"https://doi.org/10.15547/bjvm.2331","url":null,"abstract":"Trichothecenes are mycotoxins that occur in grains and can lead to acute and chronic poisoning in animals and humans. Deoxynivalenol (DON) is a type B trichothecene affecting protein synthesis, immune system, leading to brain, blood and kidney disorders. The aim of this work was to evaluate in vitro the cytotoxicity and the pathological effects of DON in short-term experiments on cells from non-tumour and tumour permanent cell lines and to compare their sensitivity. Cell cultivation of BALB/c 3T3, DEC 99, MDA-MB-231, MCF-7 and Hela cells was performed. Quantitative and qualitative methods evaluating cytotoxicity on the base of statistical and morphological analyses for determining the impact on the viability and proliferative activity were used: Neutral Red Uptake (NRU) cytotoxicity test, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test and fluorescence microscopy. The cytotoxic effect of DON was assessed after an exposure period of 24 h. DON treatment induced significant alterations in the growth and morphology of the cells, involving early and late apoptosis and necrosis signs. Statistically significant decrease of the viability of all cell lines was established at concentrations of DON starting from 1.9 µg/mL to 3.7 µg/mL, the mean IC50 concentrations were calculated. According to the IC50 values the hierarchical order of cell lines’ sensitivity was determined.","PeriodicalId":9279,"journal":{"name":"BULGARIAN JOURNAL OF VETERINARY MEDICINE","volume":"33 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89139962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T. Barakat, G. Shawky, G. Absy, M. Abd El-Rahman Ragab
This study aimed to evaluate the effect of intrauterine infusion of ceftazidime and cephapirin on uterine bacterial load and uterine horn diameter in bovine subclinical endometritis. At 7-8 weeks postpartum, a total of 122 cows suffering from subclinical endometritis were divided into three groups. Group I cows were intrauterinely (IU) infused with 2 g ceftazidime diluted with 50 mL saline; group II cows were IU infused with 2 g cephapirin diluted with 50 mL saline; and group III cows were kept as untreated control. Vaginal examination, ultrasonography and bacterial examination were done before treatment programme and later repeated twice at 10-day intervals. Staphylococcus spp., Klebsiella spp., Streptococcus spp., Escherichia coli and Proteus spp. were isolated. After the end of the treatment programme, proportions of cows infected with Staphylococcus spp. and Streptococcus spp. were significantly (P<0.05) decreased in ceftazidime and cephapirin groups. However, proportions of cows infected with Escherichia coli were significantly (P<0.05) decreased in the ceftazidime group only. Uterine bacterial loads in ceftazidime and cephapirin groups were significantly decreased (P<0.05). Mean uterine horn diameters in ceftazidime group (2.44±0.03 cm) became significantly lower (P<0.05) than those in cephapirin (2.70±0.04 cm) and control (3.06±0.06 cm) groups. Conception rate in ceftazidime group (80.95%) was significantly (P<0.05) higher than rates recorded in cephapirin (64.00%) and control (26.67%) groups. In conclusion, ceftazidime and cephapirin decreased uterine bacterial load. Moreover, ceftazidime significantly reduced uterine horn diameter compared to the other groups and was associated with significantly higher conception rate. Thus, ceftazidime is recommended for treatment of subclinical endometritis in dairy cows.
{"title":"Effect of intrauterine infusion of two cephalosporins, ceftazidime and cephapirin, on uterine bacterial load and uterine horn diameter in bovine subclinical endometritis","authors":"T. Barakat, G. Shawky, G. Absy, M. Abd El-Rahman Ragab","doi":"10.15547/bjvm.2020-0054","DOIUrl":"https://doi.org/10.15547/bjvm.2020-0054","url":null,"abstract":"This study aimed to evaluate the effect of intrauterine infusion of ceftazidime and cephapirin on uterine bacterial load and uterine horn diameter in bovine subclinical endometritis. At 7-8 weeks postpartum, a total of 122 cows suffering from subclinical endometritis were divided into three groups. Group I cows were intrauterinely (IU) infused with 2 g ceftazidime diluted with 50 mL saline; group II cows were IU infused with 2 g cephapirin diluted with 50 mL saline; and group III cows were kept as untreated control. Vaginal examination, ultrasonography and bacterial examination were done before treatment programme and later repeated twice at 10-day intervals. Staphylococcus spp., Klebsiella spp., Streptococcus spp., Escherichia coli and Proteus spp. were isolated. After the end of the treatment programme, proportions of cows infected with Staphylococcus spp. and Streptococcus spp. were significantly (P<0.05) decreased in ceftazidime and cephapirin groups. However, proportions of cows infected with Escherichia coli were significantly (P<0.05) decreased in the ceftazidime group only. Uterine bacterial loads in ceftazidime and cephapirin groups were significantly decreased (P<0.05). Mean uterine horn diameters in ceftazidime group (2.44±0.03 cm) became significantly lower (P<0.05) than those in cephapirin (2.70±0.04 cm) and control (3.06±0.06 cm) groups. Conception rate in ceftazidime group (80.95%) was significantly (P<0.05) higher than rates recorded in cephapirin (64.00%) and control (26.67%) groups. In conclusion, ceftazidime and cephapirin decreased uterine bacterial load. Moreover, ceftazidime significantly reduced uterine horn diameter compared to the other groups and was associated with significantly higher conception rate. Thus, ceftazidime is recommended for treatment of subclinical endometritis in dairy cows.","PeriodicalId":9279,"journal":{"name":"BULGARIAN JOURNAL OF VETERINARY MEDICINE","volume":"126 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88654733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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