Pub Date : 2022-03-08DOI: 10.36253/caryologia-1380
X. Yao, Haodong Liu, M. S. Tabarestani
The genus Rindera comprises about 20–25 species distributed in central eastern Europe to central Asia. Ninety-five individuals related to six Rindera were collected in 9 provinces. A total of 147 (Number of total loci) (NTL) DNA bands were produced through polymerase chain reaction amplifications (PCR) amplification of six Rindera species. These bands were produced with the combinations of 10 selective primers. The total number of amplified fragments ranged from 8 to 22. ). The predicted unbiased heterozygosity (H) varied between 0.15 (Rindera media) and 0.30 (Rindera regia). High Shannon’s information index was detected in Rindera regia. The genetic similarities between six species are estimated from 0.73 to 0.95. Clustering results showed two major clusters. According to the SRAP (Sequence-related amplified polymorphism) markers analysis, Rindera regia and Rindera media had the lowest similarity. This study also detected a significant signature of isolation by distance (Mantel test results). Present results showed that sequence-related amplified polymorphism have the potential to identify and decipher genetic affinity in Rindera species. Current results have implications in biodiversity and conservation programs.
{"title":"Morphometric analysis and genetic diversity in Rindera (Boraginaceae-Cynoglosseae) using sequence related amplified polymorphism","authors":"X. Yao, Haodong Liu, M. S. Tabarestani","doi":"10.36253/caryologia-1380","DOIUrl":"https://doi.org/10.36253/caryologia-1380","url":null,"abstract":"The genus Rindera comprises about 20–25 species distributed in central eastern Europe to central Asia. Ninety-five individuals related to six Rindera were collected in 9 provinces. A total of 147 (Number of total loci) (NTL) DNA bands were produced through polymerase chain reaction amplifications (PCR) amplification of six Rindera species. These bands were produced with the combinations of 10 selective primers. The total number of amplified fragments ranged from 8 to 22. ). The predicted unbiased heterozygosity (H) varied between 0.15 (Rindera media) and 0.30 (Rindera regia). High Shannon’s information index was detected in Rindera regia. The genetic similarities between six species are estimated from 0.73 to 0.95. Clustering results showed two major clusters. According to the SRAP (Sequence-related amplified polymorphism) markers analysis, Rindera regia and Rindera media had the lowest similarity. This study also detected a significant signature of isolation by distance (Mantel test results). Present results showed that sequence-related amplified polymorphism have the potential to identify and decipher genetic affinity in Rindera species. Current results have implications in biodiversity and conservation programs.","PeriodicalId":9634,"journal":{"name":"Caryologia","volume":"22 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2022-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83460194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
I. Patawang, Sarawut Kaewsri, S. Jantarat, P. Supanuam, Sarun Jumrusthanasan, A. Tanomtong
This study analyzed the karyological features of two bird species – Spilopelia chinensis and Tachybaptus ruficollis – from Northeastern Thailand. Mitotic chromosomes were indirectly prepared by fibroblast cell culture. The chromosomes were stained by conventional Giemsa staining and microsatellite repeat of fluorescence in situ hybridization techniques. Giemsa staining showed that the diploid chromosome number of S. chinensis was 2n=70 and T. ruficollis was 60. The types of chromosomes observed in S. chinensis were 4 large metacentric, 2 medium acrocentric, 2 small metacentric, 2 small submetacentric, 2 sex chromosomes and 58 microchromosomes; the karyotype of T. ruficollis comprised 2 large metacentric, 2 large submetacentric, 2 large acrocentric, 8 small metacentric, 4 small submetacentric, ZW sex chromosomes and 40 microchromosomes. The molecular cytogenetical features that were exhibited only on the male T. ruficollis chromosome included two microsatellites and telomeric sequences: two signals of d(CA)15 on two microchromosomes, one signal of d(GC)15 on one of the first pair, and signals of AGGGTTn sequences on each telomeric region of all macro- and microchromosomes. The karyotype formula was deduced as: 2n (70) = Lm4 + Ma2 + Sm2 + Ssm2 + 2 sex chromosomes (Sm1/Ssm1) + 58 microchromosomes for S. chinensis and 2n (60) = Lm2 +Lsm2 + La2 + Sm8 + Ssm4 + Z (Msm1) W (Ssm1) + 40 microchromosomes for T. ruficollis.
{"title":"Some molecular cytogenetic markers and classical chromosomal features of Spilopelia chinensis (Scopoli, 1786) and Tachybaptus ruficollis (Pallas, 1764) in Thailand","authors":"I. Patawang, Sarawut Kaewsri, S. Jantarat, P. Supanuam, Sarun Jumrusthanasan, A. Tanomtong","doi":"10.36253/caryologia-952","DOIUrl":"https://doi.org/10.36253/caryologia-952","url":null,"abstract":"This study analyzed the karyological features of two bird species – Spilopelia chinensis and Tachybaptus ruficollis – from Northeastern Thailand. Mitotic chromosomes were indirectly prepared by fibroblast cell culture. The chromosomes were stained by conventional Giemsa staining and microsatellite repeat of fluorescence in situ hybridization techniques. Giemsa staining showed that the diploid chromosome number of S. chinensis was 2n=70 and T. ruficollis was 60. The types of chromosomes observed in S. chinensis were 4 large metacentric, 2 medium acrocentric, 2 small metacentric, 2 small submetacentric, 2 sex chromosomes and 58 microchromosomes; the karyotype of T. ruficollis comprised 2 large metacentric, 2 large submetacentric, 2 large acrocentric, 8 small metacentric, 4 small submetacentric, ZW sex chromosomes and 40 microchromosomes. The molecular cytogenetical features that were exhibited only on the male T. ruficollis chromosome included two microsatellites and telomeric sequences: two signals of d(CA)15 on two microchromosomes, one signal of d(GC)15 on one of the first pair, and signals of AGGGTTn sequences on each telomeric region of all macro- and microchromosomes. The karyotype formula was deduced as: 2n (70) = Lm4 + Ma2 + Sm2 + Ssm2 + 2 sex chromosomes (Sm1/Ssm1) + 58 microchromosomes for S. chinensis and 2n (60) = Lm2 +Lsm2 + La2 + Sm8 + Ssm4 + Z (Msm1) W (Ssm1) + 40 microchromosomes for T. ruficollis.","PeriodicalId":9634,"journal":{"name":"Caryologia","volume":"74 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2022-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87269487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-08DOI: 10.36253/caryologia-1296
Simona Ceraulo, Milioto Vanessa, F. Dumas
LINE-1 sequences have been linked to genome evolution, plasticity and speciation; however, despite their importance, their chromosomal distribution is poorly known in primates. In this perspective, we used fluorescence in situ hybridization (FISH) to map LINE-1 probes onto two representative platyrrhine species, Aotus nancymaae (Cebidae) and Alouatta belzebul (Atelidae), both characterized with highly rearranged karyotypes, in order to investigate their chromosomal distribution and role and to better characterize the two genomes. We found centromeric enrichment of LINE-1 sequences on all biarmed and acrocentric chromosomes co-localized with heterochromatin C-positive bands. This distribution led us to hypothesize that LINE 1 sequences may have a role in the centromere architecture and karyotype organization of platyrrhine genomes.
{"title":"Centromeric enrichment of LINE-1 retrotransposon in two species of South American monkeys Alouatta belzebul and Ateles nancymaae (Platyrrhini, Primates)","authors":"Simona Ceraulo, Milioto Vanessa, F. Dumas","doi":"10.36253/caryologia-1296","DOIUrl":"https://doi.org/10.36253/caryologia-1296","url":null,"abstract":"LINE-1 sequences have been linked to genome evolution, plasticity and speciation; however, despite their importance, their chromosomal distribution is poorly known in primates. In this perspective, we used fluorescence in situ hybridization (FISH) to map LINE-1 probes onto two representative platyrrhine species, Aotus nancymaae (Cebidae) and Alouatta belzebul (Atelidae), both characterized with highly rearranged karyotypes, in order to investigate their chromosomal distribution and role and to better characterize the two genomes. We found centromeric enrichment of LINE-1 sequences on all biarmed and acrocentric chromosomes co-localized with heterochromatin C-positive bands. This distribution led us to hypothesize that LINE 1 sequences may have a role in the centromere architecture and karyotype organization of platyrrhine genomes.","PeriodicalId":9634,"journal":{"name":"Caryologia","volume":"41 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2022-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81617236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-08DOI: 10.36253/caryologia-1375
E. Falistocco, G. Marconi, L. Raggi, D. Rosellini, M. Ceccarelli, E. Albertini
Apomixis is a rather widespread phenomenon in plants. It is defined as the asexual formation of a seed from the maternal tissues of the ovule, avoiding the processes of meiosis and fertilization. Some species are facultative apomicts and form seeds by means of sexual and apomictic pathways to different extents. This is the case of Poa pratensis, the Kentucky bluegrass, which reproduces by aposporous pseudogamous facultative apomixis. This grass is one of the most studied apomictic systems, however some aspects, such as the male meiotic behavior, have not been so far investigated. In this study the process of microsporogenesis in genotypes of P. pratensis with a different mode of reproduction was investigated. The analysis revealed an almost regular meiosis in the sexual plants whereas apomictic genotypes exhibited different levels of meiotic irregularities, mainly due to cell fusion and irregular segregation in I and II division. Our data did not reveal evident connections between the extent and types of abnormalities and the components of apomixis, apomeiosis and parthenogenesis. The meiotic behavior of the examined plants was discussed in the light of their origin.
{"title":"Variation of microsporogenesis in sexual, apomictic and recombinant plants of Poa pratensis L.","authors":"E. Falistocco, G. Marconi, L. Raggi, D. Rosellini, M. Ceccarelli, E. Albertini","doi":"10.36253/caryologia-1375","DOIUrl":"https://doi.org/10.36253/caryologia-1375","url":null,"abstract":"Apomixis is a rather widespread phenomenon in plants. It is defined as the asexual formation of a seed from the maternal tissues of the ovule, avoiding the processes of meiosis and fertilization. Some species are facultative apomicts and form seeds by means of sexual and apomictic pathways to different extents. This is the case of Poa pratensis, the Kentucky bluegrass, which reproduces by aposporous pseudogamous facultative apomixis. This grass is one of the most studied apomictic systems, however some aspects, such as the male meiotic behavior, have not been so far investigated. In this study the process of microsporogenesis in genotypes of P. pratensis with a different mode of reproduction was investigated. The analysis revealed an almost regular meiosis in the sexual plants whereas apomictic genotypes exhibited different levels of meiotic irregularities, mainly due to cell fusion and irregular segregation in I and II division. Our data did not reveal evident connections between the extent and types of abnormalities and the components of apomixis, apomeiosis and parthenogenesis. The meiotic behavior of the examined plants was discussed in the light of their origin.","PeriodicalId":9634,"journal":{"name":"Caryologia","volume":"10 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2022-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90200342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-08DOI: 10.36253/caryologia-1129
Melika Tabasi, M. Sheidai, F. Koohdar, Darab Hassani
Persian walnut (Juglans regia L.), is very auspicious plant species of Iran from both economical and food points of views. Both wildly grown as well as cultivated forms of this plant species are scattered in different geographical regions of the country and are a valuable source for edible nut as well as job employment. Scattered published data on genetic diversity of this important plant species are mainly based on different molecular data analyses; therefore a meta-analysis of the same cultivars based on several different molecular markers including DNA-sequences and multi-locus markers was conducted to provide a detailed insight on genetic structure of walnuts. The results indicated a moderated genetic variability of about 40 percent in the studied cultivars; however these cultivars are genetically differentiated as revealed by Fst and AMOVA. HGT analyses revealed that the cultivars phylogeny differs to some degree by different markers and therefore a heat map was constructed to reveal the cultivars relationships based on combined molecular data. A higher Pst value was obtained compared to that of Fst genetic differentiation, therefore, it seems that local adaptation and selection have played role in the walnut cultivars’ morphological divergence. LFMM analysis identified some adaptive multi-locus alleles in the studied walnut cultivars.
{"title":"A meta-analysis of genetic divergence versus phenotypic plasticity in walnut cultivars (Juglans regia L.)","authors":"Melika Tabasi, M. Sheidai, F. Koohdar, Darab Hassani","doi":"10.36253/caryologia-1129","DOIUrl":"https://doi.org/10.36253/caryologia-1129","url":null,"abstract":"Persian walnut (Juglans regia L.), is very auspicious plant species of Iran from both economical and food points of views. Both wildly grown as well as cultivated forms of this plant species are scattered in different geographical regions of the country and are a valuable source for edible nut as well as job employment. Scattered published data on genetic diversity of this important plant species are mainly based on different molecular data analyses; therefore a meta-analysis of the same cultivars based on several different molecular markers including DNA-sequences and multi-locus markers was conducted to provide a detailed insight on genetic structure of walnuts. The results indicated a moderated genetic variability of about 40 percent in the studied cultivars; however these cultivars are genetically differentiated as revealed by Fst and AMOVA. HGT analyses revealed that the cultivars phylogeny differs to some degree by different markers and therefore a heat map was constructed to reveal the cultivars relationships based on combined molecular data. A higher Pst value was obtained compared to that of Fst genetic differentiation, therefore, it seems that local adaptation and selection have played role in the walnut cultivars’ morphological divergence. LFMM analysis identified some adaptive multi-locus alleles in the studied walnut cultivars.","PeriodicalId":9634,"journal":{"name":"Caryologia","volume":"57 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2022-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89594605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Eroğlu, E. Martin, A. Kahraman, Elif Gezer Aslan
In this study, it was aimed to determine the chromosome number of 21 Salvia L. species, to determine chromosome morphology, to reveal karyotype analysis in detail and to contribute to the cytotaxonomy of Salvia. In this context, the results are as follows: (i) the first report for the number of chromosomes of ten species, namely S. corrugata Vahl. (2n = 16), S. curviflora Benth. (2n = 16), S. darcyi J.Compton, S. greggii A.Gray, S. longifolia Nutt., S. vitifolia Benth. (2n = 22), S. subrotunda A.St.-Hil. ex Benth. (2n = 44), S. oppositiflora Ruiz & Pav. (2n = 56), S. stolonifera Benth. and S. atrocyanea Epling (2n = 60); (ii) the karyotypic variations and new chromosome numbers different from previous reports for three species, namely S. cardiophylla Benth. (2n = 36), S. cuspidata Ruiz & Pav. (2n = 44) and S. microphylla Sessé & Moc. (2n = 46); (iii) the same chromosome numbers from previous reports for eight species, namely S. campanulata Wall. ex Benth. (2n = 16), S. elegans Vahl. (2n = 20), S. involucrata Cav., S. mexicana Sessé & Moc. (2n = 22), S. apiana Jeps., S. leucophylla Greene, S. mellifera Greene (2n = 30), and S. splendens Ker Gawl. (2n = 44); (iv) the detailed chromosome measurements and karyotype analyses for all species studied for the first time; (v) the symmetrical karyotypes for all studied species; (vi) the variations resulting from dysploidy or polyploidy and discussing their reasons.
本研究旨在测定21种鼠尾草(Salvia L.)的染色体数目，确定染色体形态，进行详细的核型分析，为鼠尾草的细胞分类提供依据。在此背景下，结果如下:(1)首次报道了10个种(S.瓦楞)的染色体数目。(2n = 16);(2n = 16)，李建军，李建军，李建军。;;;;(2n = 22)， S. subrotunda a . st . hill;Benth交货。(2n = 44)， S. opposiflora Ruiz & Pav。(2n = 56)，匍匐茎;S. atrocyanea Epling (2n = 60);(ii)三种植物的核型变异和新染色体数目与文献报道不同。(2n = 36)， S. cuspidata Ruiz & Pav。(2n = 44);(2n = 46);(3) 8个物种的染色体数与前人报道的相同，即钟翅草。Benth交货。(2n = 16)，秀丽隐杆线虫;(2n = 20);墨西哥，s.s essessore & Moc。(2n = 22)， S. apiana Jeps。S. leucophylla Greene、S. mellifera Greene (2n = 30)和S. splendens Ker Gawl。(2n = 44);(iv)首次研究的所有物种的详细染色体测量和核型分析;(v)所有研究物种的对称核型;(vi)由异倍体或多倍体引起的变异及其原因的讨论。
{"title":"The new chromosomal data and karyotypic variations in genus Salvia L. (Lamiaceae): dysploidy, polyploidy and symmetrical karyotypes","authors":"H. Eroğlu, E. Martin, A. Kahraman, Elif Gezer Aslan","doi":"10.36253/caryologia-641","DOIUrl":"https://doi.org/10.36253/caryologia-641","url":null,"abstract":"In this study, it was aimed to determine the chromosome number of 21 Salvia L. species, to determine chromosome morphology, to reveal karyotype analysis in detail and to contribute to the cytotaxonomy of Salvia. In this context, the results are as follows: (i) the first report for the number of chromosomes of ten species, namely S. corrugata Vahl. (2n = 16), S. curviflora Benth. (2n = 16), S. darcyi J.Compton, S. greggii A.Gray, S. longifolia Nutt., S. vitifolia Benth. (2n = 22), S. subrotunda A.St.-Hil. ex Benth. (2n = 44), S. oppositiflora Ruiz & Pav. (2n = 56), S. stolonifera Benth. and S. atrocyanea Epling (2n = 60); (ii) the karyotypic variations and new chromosome numbers different from previous reports for three species, namely S. cardiophylla Benth. (2n = 36), S. cuspidata Ruiz & Pav. (2n = 44) and S. microphylla Sessé & Moc. (2n = 46); (iii) the same chromosome numbers from previous reports for eight species, namely S. campanulata Wall. ex Benth. (2n = 16), S. elegans Vahl. (2n = 20), S. involucrata Cav., S. mexicana Sessé & Moc. (2n = 22), S. apiana Jeps., S. leucophylla Greene, S. mellifera Greene (2n = 30), and S. splendens Ker Gawl. (2n = 44); (iv) the detailed chromosome measurements and karyotype analyses for all species studied for the first time; (v) the symmetrical karyotypes for all studied species; (vi) the variations resulting from dysploidy or polyploidy and discussing their reasons.","PeriodicalId":9634,"journal":{"name":"Caryologia","volume":"41 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2022-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76320871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-08DOI: 10.36253/caryologia-1320
Fengzhen Chen, Dongmei Li, M. Farshadfar
Lonicera L. (Caprifoliaceae) includes more than 200 species worldwide. The genus is mainly distributed in temperate to subtropical regions of the northern hemisphere: Europe, Russia, East Asia and North America. Some species are medicinal plants. Dried Lonicera flowers and buds are known as Flos Lonicera and have been a recognized herb in the traditional Chinese medicine for more than 1500 years. It has been applied for treatment of arthritis, diabetes mellitus, fever, and viral infections. Due to the importance of these plant species, we performed a combination of morphological and molecular data for this species. For this study, we used 85 randomly collected plants from six species in 6 provinces. Amplification of genomic DNA using 10 primers produced 103 bands, of which 95 were polymorphic (90.98%). The obtained high average PIC and MI values revealed high capacity of SCoT primers to detect polymorphic loci among Lonicera species. The genetic similarities of 6 collections were estimated from 0.67 to 0.90. According to the SCoT markers analysis, L. hypoleuca and L. iberica had the lowest similarity and the species of L. korolkowii and L. nummulariifolia had the highest similarity. The aims of present study are: 1) can SCoT markers identify Lonicera species, 2) what is the genetic structure of these taxa in Iran, and 3) to investigate the species inter-relationship? The present study revealed that SCoT markers can identify the species.
{"title":"Genetic variations and interspesific relationships in Lonicera L. (Caprifoliaceae), using SCoT molecular markers","authors":"Fengzhen Chen, Dongmei Li, M. Farshadfar","doi":"10.36253/caryologia-1320","DOIUrl":"https://doi.org/10.36253/caryologia-1320","url":null,"abstract":"Lonicera L. (Caprifoliaceae) includes more than 200 species worldwide. The genus is mainly distributed in temperate to subtropical regions of the northern hemisphere: Europe, Russia, East Asia and North America. Some species are medicinal plants. Dried Lonicera flowers and buds are known as Flos Lonicera and have been a recognized herb in the traditional Chinese medicine for more than 1500 years. It has been applied for treatment of arthritis, diabetes mellitus, fever, and viral infections. Due to the importance of these plant species, we performed a combination of morphological and molecular data for this species. For this study, we used 85 randomly collected plants from six species in 6 provinces. Amplification of genomic DNA using 10 primers produced 103 bands, of which 95 were polymorphic (90.98%). The obtained high average PIC and MI values revealed high capacity of SCoT primers to detect polymorphic loci among Lonicera species. The genetic similarities of 6 collections were estimated from 0.67 to 0.90. According to the SCoT markers analysis, L. hypoleuca and L. iberica had the lowest similarity and the species of L. korolkowii and L. nummulariifolia had the highest similarity. The aims of present study are: 1) can SCoT markers identify Lonicera species, 2) what is the genetic structure of these taxa in Iran, and 3) to investigate the species inter-relationship? The present study revealed that SCoT markers can identify the species. ","PeriodicalId":9634,"journal":{"name":"Caryologia","volume":"5 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2022-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87307633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-21DOI: 10.36253/caryologia-1109
Jun Wang, C. Ye, Tong Zhang, Xusheng Shi, M. Khayatnezhad, A. Shakoor
Pollen morphology of 23 species belonging to Geranium have been studied in details, which represent eight sections of two subgenera i.e., G. sect. Dissecta, Geranium, and Tuberosa of subgen. Geranium, Divaricata, Lucida, Ruberta and Trilopha of subgen. Robertium. These plant species were collected from different phytogeographical regions of Iran. The palynological investigation was done using scanning electron microscopy (SEM) techniques. Different palyno-morphological features have been observed, and the closely related species were distinguished. We used different multivariate statistical methods to reveal the species relationships. Ward clustering analyses have been done to check out the relationship among the species. The shapes of pollen grains were monad, radially symmetric, isopolar, apertures were tricolporate, and of spheroid, prolate-spheroid or sub-prolate classes. Three pollen types were recognized on the basis of differences in exine sculpturing pattern: reticulate-clavate, striate-rugulate, reticulum cristatum with clavae. Observed differences were not of diagnostic importance in subgenera and sections level. The main objective of this study is to find distinguish pollen characters in the species of the genus Geranium and to elucidate their systematics importance.
{"title":"Palynological analysis of genus Geranium (Geraniaceae) and its systematic implications using scanning electron microscopy","authors":"Jun Wang, C. Ye, Tong Zhang, Xusheng Shi, M. Khayatnezhad, A. Shakoor","doi":"10.36253/caryologia-1109","DOIUrl":"https://doi.org/10.36253/caryologia-1109","url":null,"abstract":"Pollen morphology of 23 species belonging to Geranium have been studied in details, which represent eight sections of two subgenera i.e., G. sect. Dissecta, Geranium, and Tuberosa of subgen. Geranium, Divaricata, Lucida, Ruberta and Trilopha of subgen. Robertium. These plant species were collected from different phytogeographical regions of Iran. The palynological investigation was done using scanning electron microscopy (SEM) techniques. Different palyno-morphological features have been observed, and the closely related species were distinguished. We used different multivariate statistical methods to reveal the species relationships. Ward clustering analyses have been done to check out the relationship among the species. The shapes of pollen grains were monad, radially symmetric, isopolar, apertures were tricolporate, and of spheroid, prolate-spheroid or sub-prolate classes. Three pollen types were recognized on the basis of differences in exine sculpturing pattern: reticulate-clavate, striate-rugulate, reticulum cristatum with clavae. Observed differences were not of diagnostic importance in subgenera and sections level. The main objective of this study is to find distinguish pollen characters in the species of the genus Geranium and to elucidate their systematics importance.","PeriodicalId":9634,"journal":{"name":"Caryologia","volume":"65 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2021-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77014166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Songpo Liu, Yuxuan Wang, Yuwei Song, M. Khayatnezhad, A. Minaeifar
The genus Salvia includes an enormous assemblage of nearly 1000 species dispersed around the World. Iran having 19 endemic species out of 61 is regarded as one of the important regions for Salvia diversity in Southwest Asia. Salvia species are herbaceous, rarely biennial or annual, often strongly aromatic. These species are of medicinal, commercial and horticultural value. Due to the importance of these plant species, we performed a combination of morphological and molecular data for this species. For this study, we used 145 randomly collected plants from 30 species in 18 provinces. Amplification of genomic DNA using 10 primers produced 134 bands, of which 129 were polymorphic (97.78%). The obtained high average PIC and MI values revealed high capacity of SCoT primers to detect polymorphic loci among Salvia species. The genetic similarities of 30 collections were estimated from 0.61 to 0.93. According to the SCoT markers analysis, S. tebesana and S. verticillata had the lowest similarity and the species of S. eremophila and S. santolinifolia had the highest similarity. The aims of present study are: 1) can SCoT markers identify Salvia species, 2) what is the genetic structure of these taxa in Iran, and 3) to investigate the species inter-relationship? The present study revealed that SCoT markers can identify the species.
{"title":"Genetic variations and interspesific relationships in Salvia (Lamiaceae) using SCoT molecular markers","authors":"Songpo Liu, Yuxuan Wang, Yuwei Song, M. Khayatnezhad, A. Minaeifar","doi":"10.36253/caryologia-886","DOIUrl":"https://doi.org/10.36253/caryologia-886","url":null,"abstract":"The genus Salvia includes an enormous assemblage of nearly 1000 species dispersed around the World. Iran having 19 endemic species out of 61 is regarded as one of the important regions for Salvia diversity in Southwest Asia. Salvia species are herbaceous, rarely biennial or annual, often strongly aromatic. These species are of medicinal, commercial and horticultural value. Due to the importance of these plant species, we performed a combination of morphological and molecular data for this species. For this study, we used 145 randomly collected plants from 30 species in 18 provinces. Amplification of genomic DNA using 10 primers produced 134 bands, of which 129 were polymorphic (97.78%). The obtained high average PIC and MI values revealed high capacity of SCoT primers to detect polymorphic loci among Salvia species. The genetic similarities of 30 collections were estimated from 0.61 to 0.93. According to the SCoT markers analysis, S. tebesana and S. verticillata had the lowest similarity and the species of S. eremophila and S. santolinifolia had the highest similarity. The aims of present study are: 1) can SCoT markers identify Salvia species, 2) what is the genetic structure of these taxa in Iran, and 3) to investigate the species inter-relationship? The present study revealed that SCoT markers can identify the species.","PeriodicalId":9634,"journal":{"name":"Caryologia","volume":"4 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2021-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88841301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-21DOI: 10.36253/caryologia-1248
F. Martinelli, A. Perrone, A. Dandekar
A protocol to produce transgenic shoots of Malus X domestica cv Greensleaves was optimized using two gene constructs previously used to create parthenocarpic tomato, Ino-IaaM and DefH9-IaaM. The aim was to obtain sufficient nº of transgenic shoots for in vitro multiplication, transfer to soil, grafting and testing for parthenocarpy in the next years. We investigated the effects of two modifications of a previous published protocol: 1) co-transformation with an Agrobacterium containing “VIP” genes in the gene construct and 2) two different hormones or hormone combinations. More shoot regeneration was obtained with a combination of three hormones (BA:NAA:TDZ) during co-cultivation instead of IBA and no co-transformation was performed using the VIP gene. For the DefH9-IaaM transgene, 21.04% regeneration was achieved for this treatment instead of 8.95% achieved with “IBA treatment” and 4.42% with the Agrobacterium co-transformation treatment. More shoot regeneration occurred with the combination of three hormones (BA:NAA:TDZ) instead of with only IBA and no co-transformation was performed using VIP gene. Experiments using Ino-IaaM confirmed the results shown for the DefH9-IaaM transgene. The regenerated shoots were multiplied in selective media containing kanamycin and roots were obtained.
利用先前用于单性繁殖番茄的两个基因构建体Ino-IaaM和DefH9-IaaM，优化了国产苹果(Malus X domestica cv Greensleaves)转基因苗的培育方案。目的是获得足够的转基因芽的nº，用于体外繁殖，转移到土壤中，嫁接和未来几年的孤雌性试验。我们研究了对先前发表的方案进行两项修改的效果:1)在基因构建中与含有“VIP”基因的农杆菌共转化;2)两种不同的激素或激素组合。在共培养过程中，3种激素(BA:NAA:TDZ)的组合比IBA的组合能获得更多的芽再生，而VIP基因未进行共转化。对于DefH9-IaaM转基因，该处理的再生率为21.04%，而“IBA处理”的再生率为8.95%，农杆菌共转化处理的再生率为4.42%。3种激素(BA:NAA:TDZ)联合使用比单独使用IBA的再生效果更好，VIP基因未发生共转化。使用Ino-IaaM进行的实验证实了DefH9-IaaM转基因的结果。再生芽在含卡那霉素的选择性培养基中繁殖，获得根。
{"title":"Development of a protocol for genetic transformation of Malus spp","authors":"F. Martinelli, A. Perrone, A. Dandekar","doi":"10.36253/caryologia-1248","DOIUrl":"https://doi.org/10.36253/caryologia-1248","url":null,"abstract":"A protocol to produce transgenic shoots of Malus X domestica cv Greensleaves was optimized using two gene constructs previously used to create parthenocarpic tomato, Ino-IaaM and DefH9-IaaM. The aim was to obtain sufficient nº of transgenic shoots for in vitro multiplication, transfer to soil, grafting and testing for parthenocarpy in the next years. We investigated the effects of two modifications of a previous published protocol: 1) co-transformation with an Agrobacterium containing “VIP” genes in the gene construct and 2) two different hormones or hormone combinations. More shoot regeneration was obtained with a combination of three hormones (BA:NAA:TDZ) during co-cultivation instead of IBA and no co-transformation was performed using the VIP gene. For the DefH9-IaaM transgene, 21.04% regeneration was achieved for this treatment instead of 8.95% achieved with “IBA treatment” and 4.42% with the Agrobacterium co-transformation treatment. More shoot regeneration occurred with the combination of three hormones (BA:NAA:TDZ) instead of with only IBA and no co-transformation was performed using VIP gene. Experiments using Ino-IaaM confirmed the results shown for the DefH9-IaaM transgene. The regenerated shoots were multiplied in selective media containing kanamycin and roots were obtained. ","PeriodicalId":9634,"journal":{"name":"Caryologia","volume":"220 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2021-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75731710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：