Pub Date : 2025-01-08DOI: 10.1016/j.chom.2024.12.007
Chi Chun Wong, Jun Yu
Gut bacteria could promote colorectal cancer by generating genotoxins. In a recent issue of Nature, Jans et al. identified bacterial adhesion as an additional determinant for the genotoxic activity of colibactin-producing E. coli on the colon epithelium, which could be targeted to mitigate bacteria-associated DNA damage.
{"title":"pks+ E. coli adhesins—The fine line between good and evil","authors":"Chi Chun Wong, Jun Yu","doi":"10.1016/j.chom.2024.12.007","DOIUrl":"https://doi.org/10.1016/j.chom.2024.12.007","url":null,"abstract":"Gut bacteria could promote colorectal cancer by generating genotoxins. In a recent issue of <em>Nature</em>, Jans et al. identified bacterial adhesion as an additional determinant for the genotoxic activity of colibactin-producing <em>E. coli</em> on the colon epithelium, which could be targeted to mitigate bacteria-associated DNA damage.","PeriodicalId":9693,"journal":{"name":"Cell host & microbe","volume":"53 1","pages":""},"PeriodicalIF":30.3,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142936040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-08DOI: 10.1016/j.chom.2024.12.011
Ethan T. Dehantschutter, Cormac T. Taylor
Intestinal fibrosis associated with Crohn’s disease is a serious yet poorly understood clinical complication. In this issue of Cell Host & Microbe, Ahn and colleagues provide evidence that the adherent intestinal E. coli produced the metallophore yersiniabactin, which sequesters zinc to drive intestinal fibrosis in a HIF-1α-dependent manner.
{"title":"Linking E. coli to fibrosis in Crohn’s disease","authors":"Ethan T. Dehantschutter, Cormac T. Taylor","doi":"10.1016/j.chom.2024.12.011","DOIUrl":"https://doi.org/10.1016/j.chom.2024.12.011","url":null,"abstract":"Intestinal fibrosis associated with Crohn’s disease is a serious yet poorly understood clinical complication. In this issue of <em>Cell Host & Microbe</em>, Ahn and colleagues provide evidence that the adherent intestinal <em>E. coli</em> produced the metallophore yersiniabactin, which sequesters zinc to drive intestinal fibrosis in a HIF-1α-dependent manner.","PeriodicalId":9693,"journal":{"name":"Cell host & microbe","volume":"79 1","pages":""},"PeriodicalIF":30.3,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142936187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-08DOI: 10.1016/j.chom.2024.12.008
Arren J. Liu, Jonathan B. Lynch
Jiang et al. investigate the role of the microbiota in postherpetic neuralgia (PHN), a chronic pain condition resulting from varicella-zoster virus reactivation. They identify microbiome alterations in PHN patients, linking microbes and pain sensitivity. The researchers identify butyrate-producing Roseburia intestinalis as a mediator of pain sensitivity along the “gut-brain axis.”
{"title":"Bugs take the sting out","authors":"Arren J. Liu, Jonathan B. Lynch","doi":"10.1016/j.chom.2024.12.008","DOIUrl":"https://doi.org/10.1016/j.chom.2024.12.008","url":null,"abstract":"Jiang et al. investigate the role of the microbiota in postherpetic neuralgia (PHN), a chronic pain condition resulting from varicella-zoster virus reactivation. They identify microbiome alterations in PHN patients, linking microbes and pain sensitivity. The researchers identify butyrate-producing <em>Roseburia intestinalis</em> as a mediator of pain sensitivity along the “gut-brain axis.”","PeriodicalId":9693,"journal":{"name":"Cell host & microbe","volume":"14 1","pages":""},"PeriodicalIF":30.3,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142936080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Identifying broadly reactive B precursor cells and conserved epitopes is crucial for developing a universal flu vaccine. In this study, using influenza neuraminidase (NA) mutant probes, we find that human pre-existing NA-specific memory B cells (MBCs) account for ∼0.25% of total MBCs, which are heterogeneous and dominated by class-unswitched MBCs. In addition, we identify three NA broad-inhibition monoclonal antibodies (mAbs) (BImAbs) that block the activity of NA derived from different influenza strains, including the recent cow H5N1. The cryoelectron microscopy (cryo-EM) structure shows that the BImAb targets the conserved NA enzymatic pocket and a separate epitope in the neighboring NA monomer. Furthermore, the NA BImAbs protect mice from the lethal challenge of the human pandemic H1N1 and H5N1. Our work demonstrates that the NA broad-inhibition precursor MBCs exist in healthy adults and could be targeted by the NA-based universal flu vaccine.
{"title":"Identification of a broad-inhibition influenza neuraminidase antibody from pre-existing memory B cells","authors":"Xin Wang, Huihui Kong, Bingxin Chu, Qian Yang, Chaohui Lin, Rui Liu, Changxu Chen, Yang Gao, Guojun Wang, Dayan Wang, Chen Qin, Xiaohua Ye, Lifei Yu, Xiangfei Xu, Jie Jin, Ren Sun, Hualan Chen, Xudong Wu, Zeli Zhang","doi":"10.1016/j.chom.2024.12.004","DOIUrl":"https://doi.org/10.1016/j.chom.2024.12.004","url":null,"abstract":"Identifying broadly reactive B precursor cells and conserved epitopes is crucial for developing a universal flu vaccine. In this study, using influenza neuraminidase (NA) mutant probes, we find that human pre-existing NA-specific memory B cells (MBCs) account for ∼0.25% of total MBCs, which are heterogeneous and dominated by class-unswitched MBCs. In addition, we identify three NA broad-inhibition monoclonal antibodies (mAbs) (BImAbs) that block the activity of NA derived from different influenza strains, including the recent cow H5N1. The cryoelectron microscopy (cryo-EM) structure shows that the BImAb targets the conserved NA enzymatic pocket and a separate epitope in the neighboring NA monomer. Furthermore, the NA BImAbs protect mice from the lethal challenge of the human pandemic H1N1 and H5N1. Our work demonstrates that the NA broad-inhibition precursor MBCs exist in healthy adults and could be targeted by the NA-based universal flu vaccine.","PeriodicalId":9693,"journal":{"name":"Cell host & microbe","volume":"26 1","pages":""},"PeriodicalIF":30.3,"publicationDate":"2024-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142901669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-27DOI: 10.1016/j.chom.2024.12.002
Skye R.S. Fishbein, Anna L. DeVeaux, Sakshi Khanna, Aura L. Ferreiro, James Liao, Wesley Agee, Jie Ning, Bejan Mahmud, Miranda J. Wallace, Tiffany Hink, Kimberly A. Reske, Candice Cass, Janaki Guruge, Sidh Leekha, Sunaina Rengarajan, Erik R. Dubberke, Gautam Dantas
Gastrointestinal colonization by Clostridioides difficile is common in healthcare settings and ranges in presentation from asymptomatic carriage to lethal C. difficile infection (CDI). We used a systems biology approach to investigate why patients colonized with C. difficile have a range of clinical outcomes. Microbiota humanization of germ-free mice with fecal samples from toxigenic C. difficile carriers revealed a spectrum of virulence among clinically prevalent clade 1 lineages and identified candidate taxa, including Blautia, as markers of stable colonization. Using gnotobiotic mice engrafted with defined human microbiota, we validated strain-specific CDI severity across clade 1 strains isolated from patients. Mice engrafted with a community broadly representative of colonized patients were protected from severe disease across all strains without suppression of C. difficile colonization. These results underline the capacity of gut community structure to attenuate a diversity of pathogenic strains without inhibiting colonization, providing insight into determinants of stable C. difficile carriage.
{"title":"Commensal-pathogen dynamics structure disease outcomes during Clostridioides difficile colonization","authors":"Skye R.S. Fishbein, Anna L. DeVeaux, Sakshi Khanna, Aura L. Ferreiro, James Liao, Wesley Agee, Jie Ning, Bejan Mahmud, Miranda J. Wallace, Tiffany Hink, Kimberly A. Reske, Candice Cass, Janaki Guruge, Sidh Leekha, Sunaina Rengarajan, Erik R. Dubberke, Gautam Dantas","doi":"10.1016/j.chom.2024.12.002","DOIUrl":"https://doi.org/10.1016/j.chom.2024.12.002","url":null,"abstract":"Gastrointestinal colonization by <em>Clostridioides difficile</em> is common in healthcare settings and ranges in presentation from asymptomatic carriage to lethal <em>C. difficile</em> infection (CDI). We used a systems biology approach to investigate why patients colonized with <em>C. difficile</em> have a range of clinical outcomes. Microbiota humanization of germ-free mice with fecal samples from toxigenic <em>C. difficile</em> carriers revealed a spectrum of virulence among clinically prevalent clade 1 lineages and identified candidate taxa, including <em>Blautia</em>, as markers of stable colonization. Using gnotobiotic mice engrafted with defined human microbiota, we validated strain-specific CDI severity across clade 1 strains isolated from patients. Mice engrafted with a community broadly representative of colonized patients were protected from severe disease across all strains without suppression of <em>C. difficile</em> colonization. These results underline the capacity of gut community structure to attenuate a diversity of pathogenic strains without inhibiting colonization, providing insight into determinants of stable <em>C. difficile</em> carriage.","PeriodicalId":9693,"journal":{"name":"Cell host & microbe","volume":"31 1","pages":""},"PeriodicalIF":30.3,"publicationDate":"2024-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142887313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Plant stomata open in response to blue light, allowing gas exchange and water transpiration. However, open stomata are potential entry points for pathogens. Whether plants can sense pathogens and mount defense responses upon stomatal opening and how blue-light cues are integrated to balance growth-defense trade-offs are poorly characterized. We show that the Arabidopsis blue-light photoreceptor CRYPTOCHROME 1 (CRY1) mediates various aspects of immunity, including pathogen-triggered stomatal closure as well as activation of plant immunity through a typical light-responsive protein LATE UPREGULATED IN RESPONSE TO HYALOPERONOSPORA PARASITICA (LURP1). LURP1 undergoes N-terminal palmitoylation in the presence of bacterial flagellin, prompting a change in subcellular localization from the cytoplasm to plasma membrane, where it enhances the activity of the receptor FLAGELLIN SENSING 2 (FLS2) to mediate plant defense. Collectively, these findings reveal that blue light regulates stomatal defense and highlight the dual functions of CRY1 in photosynthesis and immunity.
{"title":"The blue-light receptor CRY1 serves as a switch to balance photosynthesis and plant defense","authors":"Yuhan Hao, Zexian Zeng, Minhang Yuan, Hui Li, Shisong Guo, Yu Yang, Shushu Jiang, Eva Hawara, Jianxu Li, Peng Zhang, Jiawei Wang, Xiufang Xin, Wenbo Ma, Hongtao Liu","doi":"10.1016/j.chom.2024.12.003","DOIUrl":"https://doi.org/10.1016/j.chom.2024.12.003","url":null,"abstract":"Plant stomata open in response to blue light, allowing gas exchange and water transpiration. However, open stomata are potential entry points for pathogens. Whether plants can sense pathogens and mount defense responses upon stomatal opening and how blue-light cues are integrated to balance growth-defense trade-offs are poorly characterized. We show that the <em>Arabidopsis</em> blue-light photoreceptor CRYPTOCHROME 1 (CRY1) mediates various aspects of immunity, including pathogen-triggered stomatal closure as well as activation of plant immunity through a typical light-responsive protein LATE UPREGULATED IN RESPONSE TO <em>HYALOPERONOSPORA PARASITICA</em> (LURP1). LURP1 undergoes N-terminal palmitoylation in the presence of bacterial flagellin, prompting a change in subcellular localization from the cytoplasm to plasma membrane, where it enhances the activity of the receptor FLAGELLIN SENSING 2 (FLS2) to mediate plant defense. Collectively, these findings reveal that blue light regulates stomatal defense and highlight the dual functions of CRY1 in photosynthesis and immunity.","PeriodicalId":9693,"journal":{"name":"Cell host & microbe","volume":"305 1","pages":""},"PeriodicalIF":30.3,"publicationDate":"2024-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142887314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-26DOI: 10.1016/j.chom.2024.12.001
Jordy Evan Sulaiman, Jaron Thompson, Pak Lun Kevin Cheung, Yili Qian, Jericha Mill, Isabella James, Hanhyeok Im, Eugenio I. Vivas, Judith Simcox, Ophelia S. Venturelli
Clostridioides difficile can transiently or persistently colonize the human gut, posing a risk for infections. This colonization is influenced by complex molecular and ecological interactions with the human gut microbiota. By investigating C. difficile dynamics in human gut communities over hundreds of generations, we show patterns of stable coexistence, instability, or competitive exclusion. Lowering carbohydrate concentrations shifted a community containing C. difficile and the prevalent human gut symbiont Phocaeicola vulgatus from competitive exclusion to coexistence, facilitated by increased cross-feeding. In this environment, two key mutations in C. difficile altered its metabolic niche from proline to glucose utilization. These metabolic changes in C. difficile substantially impacted gut microbiota inter-species interactions and reduced disease severity in mice. In sum, interactions with P. vulgatus are crucial in shaping the long-term growth dynamics and evolutionary adaptations of C. difficile, offering key insights for developing anti-C. difficile strategies.
{"title":"Phocaeicola vulgatus shapes the long-term growth dynamics and evolutionary adaptations of Clostridioides difficile","authors":"Jordy Evan Sulaiman, Jaron Thompson, Pak Lun Kevin Cheung, Yili Qian, Jericha Mill, Isabella James, Hanhyeok Im, Eugenio I. Vivas, Judith Simcox, Ophelia S. Venturelli","doi":"10.1016/j.chom.2024.12.001","DOIUrl":"https://doi.org/10.1016/j.chom.2024.12.001","url":null,"abstract":"<em>Clostridioides difficile</em> can transiently or persistently colonize the human gut, posing a risk for infections. This colonization is influenced by complex molecular and ecological interactions with the human gut microbiota. By investigating <em>C. difficile</em> dynamics in human gut communities over hundreds of generations, we show patterns of stable coexistence, instability, or competitive exclusion. Lowering carbohydrate concentrations shifted a community containing <em>C. difficile</em> and the prevalent human gut symbiont <em>Phocaeicola vulgatus</em> from competitive exclusion to coexistence, facilitated by increased cross-feeding. In this environment, two key mutations in <em>C. difficile</em> altered its metabolic niche from proline to glucose utilization. These metabolic changes in <em>C. difficile</em> substantially impacted gut microbiota inter-species interactions and reduced disease severity in mice. In sum, interactions with <em>P. vulgatus</em> are crucial in shaping the long-term growth dynamics and evolutionary adaptations of <em>C. difficile</em>, offering key insights for developing anti-<em>C. difficile</em> strategies.","PeriodicalId":9693,"journal":{"name":"Cell host & microbe","volume":"23 1","pages":""},"PeriodicalIF":30.3,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142886880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-19DOI: 10.1016/j.chom.2024.11.014
Kristina Grenz, Khong-Sam Chia, Emma K. Turley, Alexa S. Tyszka, Rebecca E. Atkinson, Jacob Reeves, Martin Vickers, Martin Rejzek, Joseph F. Walker, Philip Carella
The Pseudomonas syringae species complex harbors a diverse range of pathogenic bacteria that can infect hosts across the plant kingdom. However, much of our current understanding of P. syringae is centered on its infection of flowering plants. We took a comparative approach to understand how P. syringae infects evolutionarily divergent plants. We identified P. syringae isolates causing disease in the liverwort Marchantia polymorpha, the fern Ceratopteris richardii, and the flowering plant Nicotiana benthamiana, which last shared a common ancestor >500 million years ago. Phytotoxin-enriched phylogroup (PG) 2 isolates of P. syringae are virulent in non-flowering plants, relying on type-3 effectors and the lipopeptide phytotoxin syringomycin. Ectopic syringomycin promotes tissue necrosis, activates conserved stress-related genes, and enhances in planta bacterial growth of toxin-deficient PGs in Marchantia. Collectively, our research reveals a key role for syringomycin in promoting Pseudomonas colonization, which works alongside effectors to antagonize an exceptionally wide spectrum of land plants.
{"title":"A necrotizing toxin enables Pseudomonas syringae infection across evolutionarily divergent plants","authors":"Kristina Grenz, Khong-Sam Chia, Emma K. Turley, Alexa S. Tyszka, Rebecca E. Atkinson, Jacob Reeves, Martin Vickers, Martin Rejzek, Joseph F. Walker, Philip Carella","doi":"10.1016/j.chom.2024.11.014","DOIUrl":"https://doi.org/10.1016/j.chom.2024.11.014","url":null,"abstract":"The <em>Pseudomonas syringae</em> species complex harbors a diverse range of pathogenic bacteria that can infect hosts across the plant kingdom. However, much of our current understanding of <em>P. syringae</em> is centered on its infection of flowering plants. We took a comparative approach to understand how <em>P. syringae</em> infects evolutionarily divergent plants. We identified <em>P. syringae</em> isolates causing disease in the liverwort <em>Marchantia polymorpha</em>, the fern <em>Ceratopteris richardii</em>, and the flowering plant <em>Nicotiana benthamiana</em>, which last shared a common ancestor >500 million years ago. Phytotoxin-enriched phylogroup (PG) 2 isolates of <em>P. syringae</em> are virulent in non-flowering plants, relying on type-3 effectors and the lipopeptide phytotoxin syringomycin. Ectopic syringomycin promotes tissue necrosis, activates conserved stress-related genes, and enhances <em>in planta</em> bacterial growth of toxin-deficient PGs in <em>Marchantia</em>. Collectively, our research reveals a key role for syringomycin in promoting <em>Pseudomonas</em> colonization, which works alongside effectors to antagonize an exceptionally wide spectrum of land plants.","PeriodicalId":9693,"journal":{"name":"Cell host & microbe","volume":"262 1","pages":""},"PeriodicalIF":30.3,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142849504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Approximately 20% of patients with shingles develop postherpetic neuralgia (PHN). We investigated the role of gut microbiota in shingle- and PHN-related pain. Patients with shingles or PHN exhibited significant alterations in their gut microbiota with microbial markers predicting PHN development among patients with shingles. Functionally, fecal microbiota transplantation from patients with PHN to mice heightened pain sensitivity. Administration of Roseburia intestinalis, a bacterium both depleted in patients with shingles and PHN, alleviated peripheral nerve injury-induced pain in mice. R. intestinalis enhanced vagal neurotransmission to the nucleus tractus solitarius (NTS) to suppress the central amygdala (CeA), a brain region involved in pain perception. R. intestinalis-generated butyrate activated vagal neurons through the receptor, G protein-coupled receptor 41 (GPR41). Vagal knockout of Gpr41 abolished the effects of R. intestinalis on the NTS-CeA circuit and reduced pain behaviors. Overall, we established a microbiota-based model for PHN risk assessment and identified R. intestinalis as a potential pain-alleviating probiotic.
{"title":"Roseburia intestinalis-derived butyrate alleviates neuropathic pain","authors":"Yanjun Jiang, Ziheng Huang, Wuping Sun, Jiabin Huang, Yunlong Xu, Yuliang Liao, Tingting Jin, Qing Li, Idy Hiu Ting Ho, Yidan Zou, Wenyi Zhu, Qian Li, Fenfen Qin, Xinyi Zhang, Shuqi Shi, Na Zhang, Shaomin Yang, Wenhui Xie, Songbin Wu, Likai Tan, Xiaodong Liu","doi":"10.1016/j.chom.2024.11.013","DOIUrl":"https://doi.org/10.1016/j.chom.2024.11.013","url":null,"abstract":"Approximately 20% of patients with shingles develop postherpetic neuralgia (PHN). We investigated the role of gut microbiota in shingle- and PHN-related pain. Patients with shingles or PHN exhibited significant alterations in their gut microbiota with microbial markers predicting PHN development among patients with shingles. Functionally, fecal microbiota transplantation from patients with PHN to mice heightened pain sensitivity. Administration of <em>Roseburia intestinalis</em>, a bacterium both depleted in patients with shingles and PHN, alleviated peripheral nerve injury-induced pain in mice. <em>R. intestinalis</em> enhanced vagal neurotransmission to the nucleus tractus solitarius (NTS) to suppress the central amygdala (CeA), a brain region involved in pain perception. <em>R. intestinalis-</em>generated butyrate activated vagal neurons through the receptor, G protein-coupled receptor 41 (GPR41). Vagal knockout of <em>Gpr41</em> abolished the effects of <em>R. intestinalis</em> on the NTS-CeA circuit and reduced pain behaviors. Overall, we established a microbiota-based model for PHN risk assessment and identified <em>R. intestinalis</em> as a potential pain-alleviating probiotic.","PeriodicalId":9693,"journal":{"name":"Cell host & microbe","volume":"40 1","pages":""},"PeriodicalIF":30.3,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142849559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-18DOI: 10.1016/j.chom.2024.11.012
Ju-Hyun Ahn, Marlus da Silva Pedrosa, Lacey R. Lopez, Taylor N. Tibbs, Joanna N. Jeyachandran, Emily E. Vignieri, Aaron Rothemich, Ian Cumming, Alexander D. Irmscher, Corey J. Haswell, William C. Zamboni, Yen-Rei A. Yu, Melissa Ellermann, Lee A. Denson, Janelle C. Arthur
Inflammatory bowel disease (IBD)-associated fibrosis causes significant morbidity. Mechanisms are poorly understood but implicate the microbiota, especially adherent-invasive Escherichia coli (AIEC). We previously demonstrated that AIEC producing the metallophore yersiniabactin (Ybt) promotes intestinal fibrosis in an IBD mouse model. Since macrophages interpret microbial signals and influence inflammation/tissue remodeling, we hypothesized that Ybt metal sequestration disrupts this process. Here, we show that macrophages are abundant in human IBD-fibrosis tissue and mouse fibrotic lesions, where they co-localize with AIEC. Ybt induces profibrotic gene expression in macrophages via stabilization and nuclear translocation of hypoxia-inducible factor 1-alpha (HIF-1α), a metal-dependent immune regulator. Importantly, Ybt-producing AIEC deplete macrophage intracellular zinc and stabilize HIF-1α through inhibition of zinc-dependent HIF-1α hydroxylation. HIF-1α+ macrophages localize to sites of disease activity in human IBD-fibrosis strictures and mouse fibrotic lesions, highlighting their physiological relevance. Our findings reveal microbiota-mediated metal sequestration as a profibrotic trigger targeting macrophages in the inflamed intestine.
{"title":"Intestinal E. coli-produced yersiniabactin promotes profibrotic macrophages in Crohn’s disease","authors":"Ju-Hyun Ahn, Marlus da Silva Pedrosa, Lacey R. Lopez, Taylor N. Tibbs, Joanna N. Jeyachandran, Emily E. Vignieri, Aaron Rothemich, Ian Cumming, Alexander D. Irmscher, Corey J. Haswell, William C. Zamboni, Yen-Rei A. Yu, Melissa Ellermann, Lee A. Denson, Janelle C. Arthur","doi":"10.1016/j.chom.2024.11.012","DOIUrl":"https://doi.org/10.1016/j.chom.2024.11.012","url":null,"abstract":"Inflammatory bowel disease (IBD)-associated fibrosis causes significant morbidity. Mechanisms are poorly understood but implicate the microbiota, especially adherent-invasive <em>Escherichia coli</em> (AIEC). We previously demonstrated that AIEC producing the metallophore yersiniabactin (Ybt) promotes intestinal fibrosis in an IBD mouse model. Since macrophages interpret microbial signals and influence inflammation/tissue remodeling, we hypothesized that Ybt metal sequestration disrupts this process. Here, we show that macrophages are abundant in human IBD-fibrosis tissue and mouse fibrotic lesions, where they co-localize with AIEC. Ybt induces profibrotic gene expression in macrophages via stabilization and nuclear translocation of hypoxia-inducible factor 1-alpha (HIF-1α), a metal-dependent immune regulator. Importantly, Ybt-producing AIEC deplete macrophage intracellular zinc and stabilize HIF-1α through inhibition of zinc-dependent HIF-1α hydroxylation. HIF-1α+ macrophages localize to sites of disease activity in human IBD-fibrosis strictures and mouse fibrotic lesions, highlighting their physiological relevance. Our findings reveal microbiota-mediated metal sequestration as a profibrotic trigger targeting macrophages in the inflamed intestine.","PeriodicalId":9693,"journal":{"name":"Cell host & microbe","volume":"256 1","pages":""},"PeriodicalIF":30.3,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142841504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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