Pub Date : 2024-09-19DOI: 10.1080/17425247.2024.2405207
Joana Cunha, Fátima V Ventura, Christine Charrueau, António José Ribeiro
Nucleic acid-based therapies are promising advancements in medicine. They offer unparalleled efficacy in treating previously untreatable diseases through precise gene manipulation techniques. Howev...
{"title":"Alternative routes for parenteral nucleic acid delivery and related hurdles: highlights in RNA delivery","authors":"Joana Cunha, Fátima V Ventura, Christine Charrueau, António José Ribeiro","doi":"10.1080/17425247.2024.2405207","DOIUrl":"https://doi.org/10.1080/17425247.2024.2405207","url":null,"abstract":"Nucleic acid-based therapies are promising advancements in medicine. They offer unparalleled efficacy in treating previously untreatable diseases through precise gene manipulation techniques. Howev...","PeriodicalId":12229,"journal":{"name":"Expert Opinion on Drug Delivery","volume":"25 1","pages":""},"PeriodicalIF":6.6,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142259010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-18DOI: 10.1080/17425247.2024.2405206
Alice Ng Jie Ying,Yang Fei Tan,Yee Shan Wong,Subbu Venkatraman
BACKGROUNDSustained siRNA release from nanocarriers is difficult to achieve inside the cell after entry: typically, all nanocarriers exhibit burst release of the cargo into the cytoplasm.RESEARCH DESIGN AND METHODSLayer by layer (LbL) nanoparticles (NPs) can be constructed so that they escape endosomes intact, and subsequently exhibit sustained release of the cargo. Our work quantifies intra-cellular siRNA release from multilayered NPs, evaluates mechanism behind the sustained release, and optimizes the duration of release.RESULTSIntra-cellular studies showed that nanoparticles developed with 4 layers of polyL-arginine, alternated with 3 layers of siRNA layers was able to elicit effective and prolonged SPARC knockdown activity over 21 days with a single dose treatment. For the first time, we have quantified the amounts of released siRNA in the cytoplasm and the amount of siRNA remaining inside the nanoparticles at each timepoint. Furthermore, we have correlated the amount of released siRNA within cells by LbL NPs to the cellular knockdown efficiency of multilayered delivery system.CONCLUSIONSThis methodology may provide an excellent screening tool for assessing the duration of gene silencing by various nanocarrier formulations.
{"title":"Sustained intra-cellular siRNA release from Poly(hypenCapswithspaceRetainColl1rginine) multilayered nanoparticles for prolonged gene silencing.","authors":"Alice Ng Jie Ying,Yang Fei Tan,Yee Shan Wong,Subbu Venkatraman","doi":"10.1080/17425247.2024.2405206","DOIUrl":"https://doi.org/10.1080/17425247.2024.2405206","url":null,"abstract":"BACKGROUNDSustained siRNA release from nanocarriers is difficult to achieve inside the cell after entry: typically, all nanocarriers exhibit burst release of the cargo into the cytoplasm.RESEARCH DESIGN AND METHODSLayer by layer (LbL) nanoparticles (NPs) can be constructed so that they escape endosomes intact, and subsequently exhibit sustained release of the cargo. Our work quantifies intra-cellular siRNA release from multilayered NPs, evaluates mechanism behind the sustained release, and optimizes the duration of release.RESULTSIntra-cellular studies showed that nanoparticles developed with 4 layers of polyL-arginine, alternated with 3 layers of siRNA layers was able to elicit effective and prolonged SPARC knockdown activity over 21 days with a single dose treatment. For the first time, we have quantified the amounts of released siRNA in the cytoplasm and the amount of siRNA remaining inside the nanoparticles at each timepoint. Furthermore, we have correlated the amount of released siRNA within cells by LbL NPs to the cellular knockdown efficiency of multilayered delivery system.CONCLUSIONSThis methodology may provide an excellent screening tool for assessing the duration of gene silencing by various nanocarrier formulations.","PeriodicalId":12229,"journal":{"name":"Expert Opinion on Drug Delivery","volume":"77 1","pages":""},"PeriodicalIF":6.6,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142259009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-16DOI: 10.1080/17425247.2024.2403469
Karin Somby, Valerio Campagna, Manuel V. Sanchez-Felix, Ben Forbes, Driton Vllasaliu
Published in Expert Opinion on Drug Delivery (Just accepted, 2024)
发表于《给药专家意见》（刚刚接受，2024 年）
{"title":"Development of in vitro biopharmaceutics tools for predicting the bioavailability of subcutaneously injected monoclonal antibodies and oligonucleotides","authors":"Karin Somby, Valerio Campagna, Manuel V. Sanchez-Felix, Ben Forbes, Driton Vllasaliu","doi":"10.1080/17425247.2024.2403469","DOIUrl":"https://doi.org/10.1080/17425247.2024.2403469","url":null,"abstract":"Published in Expert Opinion on Drug Delivery (Just accepted, 2024)","PeriodicalId":12229,"journal":{"name":"Expert Opinion on Drug Delivery","volume":"1 1","pages":""},"PeriodicalIF":6.6,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142259008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-13DOI: 10.1080/17425247.2024.2399706
Lucía Rodríguez-Pombo, Concepción Gallego-Fernández, Anna Kirstine Jørgensen, Carlos Javier Parramon-Teixidó, Carme Cañete-Ramirez, Maria Josep Cabañas-Poy, Abdul W. Basit, Carmen Alvarez-Lorenzo, Alvaro Goyanes
Adrenal insufficiency is usually diagnosed in children who will need lifelong hydrocortisone therapy. However, medicines for pediatrics, in terms of dosage and acceptability, are currently unavaila...
{"title":"3D printed personalized therapies for pediatric patients affected by adrenal insufficiency","authors":"Lucía Rodríguez-Pombo, Concepción Gallego-Fernández, Anna Kirstine Jørgensen, Carlos Javier Parramon-Teixidó, Carme Cañete-Ramirez, Maria Josep Cabañas-Poy, Abdul W. Basit, Carmen Alvarez-Lorenzo, Alvaro Goyanes","doi":"10.1080/17425247.2024.2399706","DOIUrl":"https://doi.org/10.1080/17425247.2024.2399706","url":null,"abstract":"Adrenal insufficiency is usually diagnosed in children who will need lifelong hydrocortisone therapy. However, medicines for pediatrics, in terms of dosage and acceptability, are currently unavaila...","PeriodicalId":12229,"journal":{"name":"Expert Opinion on Drug Delivery","volume":"16 1","pages":""},"PeriodicalIF":6.6,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142259011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
INTRODUCTIONRapid excretion of drug derivatives often results in short drug half-lives, necessitating frequent administrations. Catalytic compartments, also known as nano- and micro-reactors, offer a solution by providing confined environments for in situ production of therapeutic agents. Inspired by natural compartments, polymer-based catalytic compartments have been developed to improve reaction efficiency and enable site-specific therapeutic applications.AREAS COVEREDPolymer-based compartments provide stability, permeability control, and responsiveness to stimuli, making them ideal for generating localized compounds/signals. These sophisticated systems, engineered to carry active compounds and enable selective molecular release, represent a significant advancement in pharmaceutical research. They mimic cellular functions, creating controlled catalytic environments for bio-relevant processes. This review explores the latest advancements in synthetic catalytic compartments, focusing on design approaches, building blocks, active molecules, and key bio-applications.EXPERT OPINIONCatalytic compartments hold transformative potential in precision medicine by improving therapeutic outcomes through precise, on-site production of therapeutic agents. While promising, challenges like scalable manufacturing, biodegradability, and regulatory hurdles must be addressed to realize their full potential. Addressing these will be crucial for their successful application in healthcare.
{"title":"Redefining drug therapy: innovative approaches using catalytic compartments.","authors":"Yasemin Leyla Mustafa,Arianna Balestri,Xinan Huang,Cornelia Palivan","doi":"10.1080/17425247.2024.2403476","DOIUrl":"https://doi.org/10.1080/17425247.2024.2403476","url":null,"abstract":"INTRODUCTIONRapid excretion of drug derivatives often results in short drug half-lives, necessitating frequent administrations. Catalytic compartments, also known as nano- and micro-reactors, offer a solution by providing confined environments for in situ production of therapeutic agents. Inspired by natural compartments, polymer-based catalytic compartments have been developed to improve reaction efficiency and enable site-specific therapeutic applications.AREAS COVEREDPolymer-based compartments provide stability, permeability control, and responsiveness to stimuli, making them ideal for generating localized compounds/signals. These sophisticated systems, engineered to carry active compounds and enable selective molecular release, represent a significant advancement in pharmaceutical research. They mimic cellular functions, creating controlled catalytic environments for bio-relevant processes. This review explores the latest advancements in synthetic catalytic compartments, focusing on design approaches, building blocks, active molecules, and key bio-applications.EXPERT OPINIONCatalytic compartments hold transformative potential in precision medicine by improving therapeutic outcomes through precise, on-site production of therapeutic agents. While promising, challenges like scalable manufacturing, biodegradability, and regulatory hurdles must be addressed to realize their full potential. Addressing these will be crucial for their successful application in healthcare.","PeriodicalId":12229,"journal":{"name":"Expert Opinion on Drug Delivery","volume":"1 1","pages":""},"PeriodicalIF":6.6,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142178600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-29DOI: 10.1080/17425247.2024.2348659
Carsten Ehrhardt
Published in Expert Opinion on Drug Delivery (Ahead of Print, 2024)
发表于《给药专家意见》（2024 年，印刷版前）
{"title":"Aerosol delivery in interstitial lung diseases - breakthrough or lost cause?","authors":"Carsten Ehrhardt","doi":"10.1080/17425247.2024.2348659","DOIUrl":"https://doi.org/10.1080/17425247.2024.2348659","url":null,"abstract":"Published in Expert Opinion on Drug Delivery (Ahead of Print, 2024)","PeriodicalId":12229,"journal":{"name":"Expert Opinion on Drug Delivery","volume":"132 1","pages":""},"PeriodicalIF":6.6,"publicationDate":"2024-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140810369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-29DOI: 10.1080/17425247.2024.2348656
Hanhee Cho, Kwangmeyung Kim
Currently, cancer immunotherapy is widely used as a groundbreaking method that can completely cure advanced cancers. However, this new immunotherapy has the challenge of low patient response, which...
{"title":"Multi-functional nanomedicines for combinational cancer immunotherapy that transform cold tumors to hot tumors","authors":"Hanhee Cho, Kwangmeyung Kim","doi":"10.1080/17425247.2024.2348656","DOIUrl":"https://doi.org/10.1080/17425247.2024.2348656","url":null,"abstract":"Currently, cancer immunotherapy is widely used as a groundbreaking method that can completely cure advanced cancers. However, this new immunotherapy has the challenge of low patient response, which...","PeriodicalId":12229,"journal":{"name":"Expert Opinion on Drug Delivery","volume":"33 1","pages":""},"PeriodicalIF":6.6,"publicationDate":"2024-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140842547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-17DOI: 10.1080/17425247.2024.2343894
Jeffry G. Weers
Up to 50% of asthma/COPD patients make critical errors in dose preparation and dose inhalation with current marketed DPIs which negatively impact clinical outcomes. Others fail to adhere to their c...
{"title":"Design of dry powder inhalers to improve patient outcomes: it’s not just about the device","authors":"Jeffry G. Weers","doi":"10.1080/17425247.2024.2343894","DOIUrl":"https://doi.org/10.1080/17425247.2024.2343894","url":null,"abstract":"Up to 50% of asthma/COPD patients make critical errors in dose preparation and dose inhalation with current marketed DPIs which negatively impact clinical outcomes. Others fail to adhere to their c...","PeriodicalId":12229,"journal":{"name":"Expert Opinion on Drug Delivery","volume":"12 1","pages":""},"PeriodicalIF":6.6,"publicationDate":"2024-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140617362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-16DOI: 10.1080/17425247.2024.2343882
Abed Alqader Ibrahim, Hamdi Nsairat, Mazen Al-Sulaibi, Mohamed El-Tanani, Areej M. Jaber, Zainab Lafi, Rahmeh Barakat, Duaa Azmi Abuarqoub, Ismail Sami Mahmoud, Sherine O Obare, Alaa A. A. Aljabali, Alaaldin M. Alkilany, Walhan Alshaer
Doxorubicin (DOX) emerges as a cornerstone in the arsenal of potent chemotherapeutic agents. Yet, the clinical deployment of DOX is tarnished by its proclivity to induce severe cardiotoxic effects,...
{"title":"Doxorubicin conjugates: a practical approach for its cardiotoxicity alleviation","authors":"Abed Alqader Ibrahim, Hamdi Nsairat, Mazen Al-Sulaibi, Mohamed El-Tanani, Areej M. Jaber, Zainab Lafi, Rahmeh Barakat, Duaa Azmi Abuarqoub, Ismail Sami Mahmoud, Sherine O Obare, Alaa A. A. Aljabali, Alaaldin M. Alkilany, Walhan Alshaer","doi":"10.1080/17425247.2024.2343882","DOIUrl":"https://doi.org/10.1080/17425247.2024.2343882","url":null,"abstract":"Doxorubicin (DOX) emerges as a cornerstone in the arsenal of potent chemotherapeutic agents. Yet, the clinical deployment of DOX is tarnished by its proclivity to induce severe cardiotoxic effects,...","PeriodicalId":12229,"journal":{"name":"Expert Opinion on Drug Delivery","volume":"53 1","pages":""},"PeriodicalIF":6.6,"publicationDate":"2024-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140598706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-15DOI: 10.1080/17425247.2024.2340661
Cristina Yus, Enrique Gámez, Manuel Arruebo
Different active and passive strategies have been developed to fight against pathogenic bacteria. Those actions are undertaken to reduce the bacterial burden while minimizing the possibilities to d...
{"title":"Expert opinion on antimicrobial therapies: is there enough scientific evidence to state that targeted therapies outperform non-targeted ones?","authors":"Cristina Yus, Enrique Gámez, Manuel Arruebo","doi":"10.1080/17425247.2024.2340661","DOIUrl":"https://doi.org/10.1080/17425247.2024.2340661","url":null,"abstract":"Different active and passive strategies have been developed to fight against pathogenic bacteria. Those actions are undertaken to reduce the bacterial burden while minimizing the possibilities to d...","PeriodicalId":12229,"journal":{"name":"Expert Opinion on Drug Delivery","volume":"12 1","pages":""},"PeriodicalIF":6.6,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140598573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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