Pub Date : 2022-06-30DOI: 10.47866/2615-9252/vjfc.3913
Ngan Le Viet, Huyen Trang Luu Thi, Xuan Nguyen Thi, Trang Vu Thi
The study uses rapid aging and real-condition methods to assess the stability of frozen meat, yogurt and functional foods. The method of real conditions is applied to frozen meat and yogurt with the following factors being: temperature: -20°C ± 2°C; humidity: 100% for frozen meat and temperature: 4°C ± 2°C; humidity: > 80% for yogurt. The accelerated aging method is applied to functional foods in conditions: 35 ± 2°C, 45 ± 2°C, 55 ± 2°C, and humidity: 70 ± 5% in parallel assessment combination with real-time monitoring at 25 ± 2°C, and humidity 70 ± 5%. The results showed that the yogurt sample reached stability until the 45th day and did not reach from the 47th day due to layering and deposits at the bottom of the box. The frozen meat sample remained stable when monitored until the 12.6 months with all indicators. Functional food samples were estimated to reach stability of up to 40.3 months when using the Van't Hoff formula, 32.0 months when using the Q10 coefficient, and 36.8 months when monitored in real conditions. The results of the study indicate that the less stable criteria on each sample background are the basis for selecting evaluation criteria for subsequent studies that contribute to reducing the cost of monitoring. The difference in the time it takes to estimate the stability of the product when using different methods also helps manufacturers consider the appropriate evaluation method for their products.
{"title":"Research for stability of food products and functional foods","authors":"Ngan Le Viet, Huyen Trang Luu Thi, Xuan Nguyen Thi, Trang Vu Thi","doi":"10.47866/2615-9252/vjfc.3913","DOIUrl":"https://doi.org/10.47866/2615-9252/vjfc.3913","url":null,"abstract":"The study uses rapid aging and real-condition methods to assess the stability of frozen meat, yogurt and functional foods. The method of real conditions is applied to frozen meat and yogurt with the following factors being: temperature: -20°C ± 2°C; humidity: 100% for frozen meat and temperature: 4°C ± 2°C; humidity: > 80% for yogurt. The accelerated aging method is applied to functional foods in conditions: 35 ± 2°C, 45 ± 2°C, 55 ± 2°C, and humidity: 70 ± 5% in parallel assessment combination with real-time monitoring at 25 ± 2°C, and humidity 70 ± 5%. The results showed that the yogurt sample reached stability until the 45th day and did not reach from the 47th day due to layering and deposits at the bottom of the box. The frozen meat sample remained stable when monitored until the 12.6 months with all indicators. Functional food samples were estimated to reach stability of up to 40.3 months when using the Van't Hoff formula, 32.0 months when using the Q10 coefficient, and 36.8 months when monitored in real conditions. The results of the study indicate that the less stable criteria on each sample background are the basis for selecting evaluation criteria for subsequent studies that contribute to reducing the cost of monitoring. The difference in the time it takes to estimate the stability of the product when using different methods also helps manufacturers consider the appropriate evaluation method for their products.","PeriodicalId":12896,"journal":{"name":"Heavy metals and arsenic concentrations in water, agricultural soil, and rice in Ngan Son district, Bac Kan province, Vietnam","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75109854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-28DOI: 10.47866/2615-9252/vjfc.3910
Như Quỳnh Bach Thị, Tuyet Lan Ninh Thị, Minh Huyen Nguyen Thi, Men Do Thi, Le Pham Thi, Dam Linh Mai Thi
Bifidobacterium is anerobic bacterium which is popular in human and animal intestine. B. infantis was successfully isolated from a new-born child’s fecal sample previously. In this study, the probiotic characteristics of this strain such as: extracellular enzyme production ability; antibiotic activity against food-born pathogen like E. coli, S. aureus; organic acid production and bile-salt sufferance were evaluated. The results showed that the isolated can produce amylase and protease enzyme, do not produce cellulase enzyme; the organic acids also were produced during development of bacterium. The bacterium can against E. coli and S. aureus and can grow in the media containing up to 1% of bile salt. Results of this study are the basis for further studies to apply these bacteria as a source of probiotic beneficial to human health in Vietnam.
{"title":"Probiotic characteristics of Bifidobacterium infantis isolated in Vietnam","authors":"Như Quỳnh Bach Thị, Tuyet Lan Ninh Thị, Minh Huyen Nguyen Thi, Men Do Thi, Le Pham Thi, Dam Linh Mai Thi","doi":"10.47866/2615-9252/vjfc.3910","DOIUrl":"https://doi.org/10.47866/2615-9252/vjfc.3910","url":null,"abstract":"Bifidobacterium is anerobic bacterium which is popular in human and animal intestine. B. infantis was successfully isolated from a new-born child’s fecal sample previously. In this study, the probiotic characteristics of this strain such as: extracellular enzyme production ability; antibiotic activity against food-born pathogen like E. coli, S. aureus; organic acid production and bile-salt sufferance were evaluated. The results showed that the isolated can produce amylase and protease enzyme, do not produce cellulase enzyme; the organic acids also were produced during development of bacterium. The bacterium can against E. coli and S. aureus and can grow in the media containing up to 1% of bile salt. Results of this study are the basis for further studies to apply these bacteria as a source of probiotic beneficial to human health in Vietnam.","PeriodicalId":12896,"journal":{"name":"Heavy metals and arsenic concentrations in water, agricultural soil, and rice in Ngan Son district, Bac Kan province, Vietnam","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84776404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Chemicals that are not subject to hygienic standards and yet are found in rice can be classified as unintentionally present chemicals in food products. However, these chemicals can pose a potential health hazard, which requires an assessment of the risk they represent. For this purpose, it is necessary to select priority potentially dangerous unintentionally present chemicals in rice. The study included five samples of rice from Cambodia, India, and Russia. Chemical element content studies were conducted on Agilent 7900 mass spectrometer (Agilent Technologies, Japan/Singapore) with an octopole collision/reaction cell (ORS) using the semi-quantitative analysis program for the mass spectrometer. The selection of priority unintentionally present chemical contaminants in rice was conducted in three stages. The researchers applied specific criteria to include chemicals in the further assessment at each stage. As a result of the evaluation of the selection of priority unintentionally present chemicals contained in rice sold through retail chains, a potential hazard category (PHC) was established, i.e., an integral characteristic of the potential hazard of an unintentionally present chemical contained in a food product, covering the possibility of ingestion of a specific chemical and its toxicity. Thus, in accordance with the PHC, aluminum was identified as priority potentially hazardous chemical contained in rice for further risk assessment since it corresponded to category I of the potential hazard.
{"title":"Selection of priority unintentionally present chemical contaminants based on the example of rice retailed in the Russian Federation","authors":"Vladimirovna Zaitseva Nina, Zalmanovich Shur Pavel, Vladimirovich Suvorov Dmitry, Evgenjevich Zelenkin Sergey, Vladimirovna Nedoshitova Anna, Vjacheslavovna Stenno Elena","doi":"10.47866/2615-9252/vjfc.3907","DOIUrl":"https://doi.org/10.47866/2615-9252/vjfc.3907","url":null,"abstract":"Chemicals that are not subject to hygienic standards and yet are found in rice can be classified as unintentionally present chemicals in food products. However, these chemicals can pose a potential health hazard, which requires an assessment of the risk they represent. For this purpose, it is necessary to select priority potentially dangerous unintentionally present chemicals in rice. The study included five samples of rice from Cambodia, India, and Russia. Chemical element content studies were conducted on Agilent 7900 mass spectrometer (Agilent Technologies, Japan/Singapore) with an octopole collision/reaction cell (ORS) using the semi-quantitative analysis program for the mass spectrometer. The selection of priority unintentionally present chemical contaminants in rice was conducted in three stages. The researchers applied specific criteria to include chemicals in the further assessment at each stage. As a result of the evaluation of the selection of priority unintentionally present chemicals contained in rice sold through retail chains, a potential hazard category (PHC) was established, i.e., an integral characteristic of the potential hazard of an unintentionally present chemical contained in a food product, covering the possibility of ingestion of a specific chemical and its toxicity. Thus, in accordance with the PHC, aluminum was identified as priority potentially hazardous chemical contained in rice for further risk assessment since it corresponded to category I of the potential hazard.","PeriodicalId":12896,"journal":{"name":"Heavy metals and arsenic concentrations in water, agricultural soil, and rice in Ngan Son district, Bac Kan province, Vietnam","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75382658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-27DOI: 10.47866/2615-9252/vjfc.3911
T. Nguyen Duc, Trang Nguyen Hoang Thu, Van Anh Nguyen Thi
Mango leaves were extracted by Soxhlet with 60% ethanol, the obtained solution was distilled to remove the solvent under reduced pressure. The results showed that when using the maximum allowable dose, there were no dead mice. Experimenting on blood glucose-lowering effects on diabetic white mice obtained the results: with a dose of 500 mg/kg giving the best therapeutic effect, even slightly better than the positive control drug gliclazide, the blood glucose level decreased immediately after three days. After 21 days reduced to 52.5% and brought the blood glucose index to normal. This is a new direction in the use of drugs of plant origin in general and Mango leaves in particular.
{"title":"Study on the hypoglycemic effect of mango leaf extract grown in Son La","authors":"T. Nguyen Duc, Trang Nguyen Hoang Thu, Van Anh Nguyen Thi","doi":"10.47866/2615-9252/vjfc.3911","DOIUrl":"https://doi.org/10.47866/2615-9252/vjfc.3911","url":null,"abstract":"Mango leaves were extracted by Soxhlet with 60% ethanol, the obtained solution was distilled to remove the solvent under reduced pressure. The results showed that when using the maximum allowable dose, there were no dead mice. Experimenting on blood glucose-lowering effects on diabetic white mice obtained the results: with a dose of 500 mg/kg giving the best therapeutic effect, even slightly better than the positive control drug gliclazide, the blood glucose level decreased immediately after three days. After 21 days reduced to 52.5% and brought the blood glucose index to normal. This is a new direction in the use of drugs of plant origin in general and Mango leaves in particular.","PeriodicalId":12896,"journal":{"name":"Heavy metals and arsenic concentrations in water, agricultural soil, and rice in Ngan Son district, Bac Kan province, Vietnam","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74885166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-06DOI: 10.47866/2615-9252/vjfc.3909
Anh Vu Truong Huynh, Khue Tu Nguyen Hoang, Ha Huynh Yen, Hai Chu Van
After Salmonella strains were isolated from food samples collected randomly at conventional markets in several districts in Ho Chi Minh city, we evaluated antibiotic resistance by Kirby-Bauer methods, and the serotype name was assigned according to ISO/TR 6579-3:2014 and class 1 integron was investigated by PCR technique. As a result, there were seven distinguished serovars, including S. Kentucky (8 strains); S. Infantis (4 strains); S. Agona and S. Potsdam (2 strains); S. Saintpaul, S. Braenderup, S. Indiana (01 strain); OMF:1,z6:UT and 7:1,z6:UT (01 strain). The rate of multidrug-resistance Salmonella serovars carrying class 1 integron was 100% (21/21). The gene cassette region of class 1 integron accounted for 85.71% (18/21). The presence of mobile genetic factors in Salmonella in the study suggests that the bacteria can transmit or receive antibiotic resistance genes from other bacterial species in the natural environment. In addition, the research results provide scientific evidence for management decisions and raise awareness of effective antibiotic use in Ho Chi Minh City, Vietnam.
从胡志明市多个地区的传统市场随机采集的食品中分离沙门氏菌，采用Kirby-Bauer法进行耐药性鉴定，并根据ISO/TR 6579-3:2014进行血清型命名，采用PCR技术检测1类整合子。结果，共有7种不同的血清型，包括肯塔基沙门氏菌(8株);婴儿链球菌(4株);S. Agona和S. Potsdam(2株);S. saint - paul, S. Braenderup, S. Indiana(01菌株);OMF:1,z6:UT和7:1,z6:UT(01菌株)。携带1类整合子的多药耐药血清型沙门氏菌检出率为100%(21/21)。1类整合子基因盒区占85.71%(18/21)。研究中沙门氏菌中移动遗传因子的存在表明，该细菌可以在自然环境中传播或接受来自其他细菌物种的抗生素抗性基因。此外，研究结果为管理决策提供了科学依据，并提高了越南胡志明市有效使用抗生素的认识。
{"title":"Detection of class 1 integron-associated gene cassettes of multi-drug resistant Salmonella strains isolated from food at conventional markets in Ho Chi Minh city","authors":"Anh Vu Truong Huynh, Khue Tu Nguyen Hoang, Ha Huynh Yen, Hai Chu Van","doi":"10.47866/2615-9252/vjfc.3909","DOIUrl":"https://doi.org/10.47866/2615-9252/vjfc.3909","url":null,"abstract":"After Salmonella strains were isolated from food samples collected randomly at conventional markets in several districts in Ho Chi Minh city, we evaluated antibiotic resistance by Kirby-Bauer methods, and the serotype name was assigned according to ISO/TR 6579-3:2014 and class 1 integron was investigated by PCR technique. As a result, there were seven distinguished serovars, including S. Kentucky (8 strains); S. Infantis (4 strains); S. Agona and S. Potsdam (2 strains); S. Saintpaul, S. Braenderup, S. Indiana (01 strain); OMF:1,z6:UT and 7:1,z6:UT (01 strain). The rate of multidrug-resistance Salmonella serovars carrying class 1 integron was 100% (21/21). The gene cassette region of class 1 integron accounted for 85.71% (18/21). The presence of mobile genetic factors in Salmonella in the study suggests that the bacteria can transmit or receive antibiotic resistance genes from other bacterial species in the natural environment. In addition, the research results provide scientific evidence for management decisions and raise awareness of effective antibiotic use in Ho Chi Minh City, Vietnam.","PeriodicalId":12896,"journal":{"name":"Heavy metals and arsenic concentrations in water, agricultural soil, and rice in Ngan Son district, Bac Kan province, Vietnam","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72854130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-06DOI: 10.47866/2615-9252/vjfc.3912
Thanh Hoa Mac Thi, Thanh Nguyen Ha, Ngoc Lan Dang Thi, Thanh Ha Pham Thi, Thanh An Vu Thi, Khanh Cao Cong
Glutaraldehyde (GA) is commonly used to disinfect surfaces and equipment in industries, agriculture, healthcare, laboratories, etc. To evaluate the quality of products containing GA, a HPLC method using 2,4-diphenylhydrazine derivative was developed. The analyte was separated by CN column with isocratic of acetonitrile and phosphoric acid 0,1% (ratio 50 : 50) and detected by PDA detector using a wavelength of 360 nm. The method was validated for specificity, linear curves, precision and accuracy which meet the requirements of AOAC.
{"title":"High performance liquid chromatography analytical method for glutaraldehyde determination in disinfectants","authors":"Thanh Hoa Mac Thi, Thanh Nguyen Ha, Ngoc Lan Dang Thi, Thanh Ha Pham Thi, Thanh An Vu Thi, Khanh Cao Cong","doi":"10.47866/2615-9252/vjfc.3912","DOIUrl":"https://doi.org/10.47866/2615-9252/vjfc.3912","url":null,"abstract":"Glutaraldehyde (GA) is commonly used to disinfect surfaces and equipment in industries, agriculture, healthcare, laboratories, etc. To evaluate the quality of products containing GA, a HPLC method using 2,4-diphenylhydrazine derivative was developed. The analyte was separated by CN column with isocratic of acetonitrile and phosphoric acid 0,1% (ratio 50 : 50) and detected by PDA detector using a wavelength of 360 nm. The method was validated for specificity, linear curves, precision and accuracy which meet the requirements of AOAC.","PeriodicalId":12896,"journal":{"name":"Heavy metals and arsenic concentrations in water, agricultural soil, and rice in Ngan Son district, Bac Kan province, Vietnam","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84403151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-04DOI: 10.47866/2615-9252/vjfc.3935
Huyen My Dang Thi, Ngoc Ly Nguyen Thi, Nhi Do Yen, Diu Dinh Thi, Lan Phuong Kieu Thi, Anh Hoang Quoc, Nguyen Pham Hoang, Quynh Anh Pham Thi, Son Tran Cao, Minh Thu Nguyen Thi, Huy Nguyen Quang, Anh Huong Nguyen Thi
The quantification of glucosamine in dietary supplements was investigated by using capillary with capacitively coupled contactless conductivity detection (CE-C4D). Optimal conditions included electrophoresis buffer solution 10 mM Tris/Ace; pH = 5,0; sample injection height 25 cm; sample injection time 30 s; separation voltage 20 kV. The limit of detection (LOD) and the limit of quantification (LOQ) of glucosamine were 8.00 ppm and 26.67 ppm, respectively. Calibration curve was constructed over the range from 20 to 1000 ppm giving a good linear correlation coefficient (R2 = 0.9993). The relative standard deviation (RSD) ranged from 1.28% to 2.33% (n = 3) and the recovery efficiency ranged from 95.2 to 102.3% which indicated good precision and trueness of our method. The method was applied to determine glucosamine in 4 dietary supplement samples randomly collected at the local markets. The CE-C4D results were compared with those obtained by reference HPLC-FLD method, showing good agreement between the two methods (relative error < 15%).
{"title":"Determination of glucosamine in dietary supplement samples by capillary electrophoresis using capacitively coupled contactless conductivity detector (CE-C4D)","authors":"Huyen My Dang Thi, Ngoc Ly Nguyen Thi, Nhi Do Yen, Diu Dinh Thi, Lan Phuong Kieu Thi, Anh Hoang Quoc, Nguyen Pham Hoang, Quynh Anh Pham Thi, Son Tran Cao, Minh Thu Nguyen Thi, Huy Nguyen Quang, Anh Huong Nguyen Thi","doi":"10.47866/2615-9252/vjfc.3935","DOIUrl":"https://doi.org/10.47866/2615-9252/vjfc.3935","url":null,"abstract":"The quantification of glucosamine in dietary supplements was investigated by using capillary with capacitively coupled contactless conductivity detection (CE-C4D). Optimal conditions included electrophoresis buffer solution 10 mM Tris/Ace; pH = 5,0; sample injection height 25 cm; sample injection time 30 s; separation voltage 20 kV. The limit of detection (LOD) and the limit of quantification (LOQ) of glucosamine were 8.00 ppm and 26.67 ppm, respectively. Calibration curve was constructed over the range from 20 to 1000 ppm giving a good linear correlation coefficient (R2 = 0.9993). The relative standard deviation (RSD) ranged from 1.28% to 2.33% (n = 3) and the recovery efficiency ranged from 95.2 to 102.3% which indicated good precision and trueness of our method. The method was applied to determine glucosamine in 4 dietary supplement samples randomly collected at the local markets. The CE-C4D results were compared with those obtained by reference HPLC-FLD method, showing good agreement between the two methods (relative error < 15%).","PeriodicalId":12896,"journal":{"name":"Heavy metals and arsenic concentrations in water, agricultural soil, and rice in Ngan Son district, Bac Kan province, Vietnam","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89675667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-03DOI: 10.47866/2615-9252/vjfc.3942
Lan Anh Hoang Thi, Oanh Nguyen Thi, Diu Dinh Thi, Nhi Do Yen, Bach Pham Gia, Trang Vu Thi, Anh Huong Nguyen Thi, Hong Hao Le Thi
Dietary supplements are currently popular because of their natural origin, especially male enhancement products. However, some of these products have been found to contain erectile dysfunction drugs that inhibit the enzyme phosphodiesterase-5 (PDE-5), leading to adverse effects on human health. In this study, capillary electrophoresis with capacitively coupled contactless conductivity detection (CE-C4D) was used to determine four PDE-5 inhibitors (including acetyl vardenafil, homosildenafil, hydroxy acetildenafil and mirodenafil) in dietary supplement samples. The optimum analytical conditions included electrolyte solution of 20 mM arginine (Arg)/acetic acid (Ace)/acetonitrile (ACN) 20% pH 6.5, silica capillary 50 μm inner diameter with effective length of 50 cm, separation voltage of 20kV, siphoning hydrodynamic injection at a height of 25 cm for 30 s. At the optimum analytical conditions, the limits of detection of acetyl vardenafil, homosildenafil, hydroxy acetildenafil, and mirodenafil were 2.1, 1.6, 2.3, and 1.3 mg/L, respectively. The analysis of four PDE-5 inhibitors by CE-C4D also showed good repeatability (< 5 %) and recovery (> 97 %). This method was further applied to simultaneously determine the amount of acetyl vardenafil, homosildenafil, hydroxy acetildenafil, and mirodenafil in three functional products. All four PDE-5 inhibitors were not detected in these samples. To demonstrate the utility of this method, the mixture of four PDE-5 inhibitors standard solutions was added to three samples. The recovery was between 98.8 % and 100.6%, illustrating the potential applicability of the method of determining PDE-5 inhibitors in dietary supplement samples.
{"title":"Simultaneous determination of acetyl vardenafil, homosildenafil, hydroxy acetildenafil and mirodenafil by CE-C4D","authors":"Lan Anh Hoang Thi, Oanh Nguyen Thi, Diu Dinh Thi, Nhi Do Yen, Bach Pham Gia, Trang Vu Thi, Anh Huong Nguyen Thi, Hong Hao Le Thi","doi":"10.47866/2615-9252/vjfc.3942","DOIUrl":"https://doi.org/10.47866/2615-9252/vjfc.3942","url":null,"abstract":"Dietary supplements are currently popular because of their natural origin, especially male enhancement products. However, some of these products have been found to contain erectile dysfunction drugs that inhibit the enzyme phosphodiesterase-5 (PDE-5), leading to adverse effects on human health. In this study, capillary electrophoresis with capacitively coupled contactless conductivity detection (CE-C4D) was used to determine four PDE-5 inhibitors (including acetyl vardenafil, homosildenafil, hydroxy acetildenafil and mirodenafil) in dietary supplement samples. The optimum analytical conditions included electrolyte solution of 20 mM arginine (Arg)/acetic acid (Ace)/acetonitrile (ACN) 20% pH 6.5, silica capillary 50 μm inner diameter with effective length of 50 cm, separation voltage of 20kV, siphoning hydrodynamic injection at a height of 25 cm for 30 s. At the optimum analytical conditions, the limits of detection of acetyl vardenafil, homosildenafil, hydroxy acetildenafil, and mirodenafil were 2.1, 1.6, 2.3, and 1.3 mg/L, respectively. The analysis of four PDE-5 inhibitors by CE-C4D also showed good repeatability (< 5 %) and recovery (> 97 %). This method was further applied to simultaneously determine the amount of acetyl vardenafil, homosildenafil, hydroxy acetildenafil, and mirodenafil in three functional products. All four PDE-5 inhibitors were not detected in these samples. To demonstrate the utility of this method, the mixture of four PDE-5 inhibitors standard solutions was added to three samples. The recovery was between 98.8 % and 100.6%, illustrating the potential applicability of the method of determining PDE-5 inhibitors in dietary supplement samples.","PeriodicalId":12896,"journal":{"name":"Heavy metals and arsenic concentrations in water, agricultural soil, and rice in Ngan Son district, Bac Kan province, Vietnam","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82628125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-30DOI: 10.47866/2615-9252/vjfc.3885
Thanh An Vu Thi, Khanh Cao Cong, Thanh Hoa Mac Thi, Son Tran Hung, Thanh Thuy Do Thi, Huyen Nguyen Thi
Hesperidin is the main active ingredient in the flavonoid group, accounting for about 3 % of Chenbi. According to studies, hesperidin has anti-oxidant, analgesic, and anti-inflammatory effects on the nervous system as well as enhancing the body's resistance. Chenbi is a simple medicine, easy to obtain, and uses medicinal material for the potential extraction and isolation of Hesperin with high yield. The study developed and validated the process of determining hesperidin on medicinal samples and raw materials by HPLC. The study was successful in making the active ingredient hesperidin with a purity > 95 % (HPLC) sufficient as a condition to be used as a referenced material. Extraction and isolation process using 70 % ethanol extraction solvent with extraction method is reflux heating then cleaning with activated carbon and purified on chromatographic equipment to obtain high recovery efficiency, simple and economical process.
{"title":"Development of the process for extracting and isolating hesperidin from Chenbi to establish as a reference standard","authors":"Thanh An Vu Thi, Khanh Cao Cong, Thanh Hoa Mac Thi, Son Tran Hung, Thanh Thuy Do Thi, Huyen Nguyen Thi","doi":"10.47866/2615-9252/vjfc.3885","DOIUrl":"https://doi.org/10.47866/2615-9252/vjfc.3885","url":null,"abstract":"Hesperidin is the main active ingredient in the flavonoid group, accounting for about 3 % of Chenbi. According to studies, hesperidin has anti-oxidant, analgesic, and anti-inflammatory effects on the nervous system as well as enhancing the body's resistance. Chenbi is a simple medicine, easy to obtain, and uses medicinal material for the potential extraction and isolation of Hesperin with high yield. The study developed and validated the process of determining hesperidin on medicinal samples and raw materials by HPLC. The study was successful in making the active ingredient hesperidin with a purity > 95 % (HPLC) sufficient as a condition to be used as a referenced material. Extraction and isolation process using 70 % ethanol extraction solvent with extraction method is reflux heating then cleaning with activated carbon and purified on chromatographic equipment to obtain high recovery efficiency, simple and economical process.","PeriodicalId":12896,"journal":{"name":"Heavy metals and arsenic concentrations in water, agricultural soil, and rice in Ngan Son district, Bac Kan province, Vietnam","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72651104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-29DOI: 10.47866/2615-9252/vjfc.3886
N. Phạm, Hoang Luu, Trang Vu Thu, To Quynh Cung Thi
Honey is a natural product with a variety of uses. The antibacterial activity of honey is one of the key factors that make honey beneficial. The study aims to compare some quality index and antibacterial capacity of some main kinds of honey in Vietnam (determined by the diameters of the inhibition zones, the minimum inhibitory concentration - MIC, and the bactericidal concentrations - MBC). The results showed that the honey samples met the quality requirements of TCVN 12605:2019 for honey, including free reducing sugar content (ranging 67 - 72 g/100 g), diastase activity (ranging from 5.58 ± 0.46 to 25.27 ± 0.05 Schad), HMF (5-Hydroxymethylfurfural) content (ranging from 18.06 ± 0.09 to 23.80 ± 0.25 mg/kg). All honey samples exhibited antibacterial activity against all eight strains of bacteria studied as follows: the diameters of the inhibition zones ranged from 8.50 ± 0.29 to 22.33 ± 1.53 (mm), MIC ranged from 0.15 ± 0.00 to 0.90 ± 0.14 (g/mL). Honey from coffee and rubber flowers showed strong bactericidal ability at MBC concentration from 0.25 g/mL.
{"title":"Comparison of antibacterial activities of some kinds of honey in Vietnam","authors":"N. Phạm, Hoang Luu, Trang Vu Thu, To Quynh Cung Thi","doi":"10.47866/2615-9252/vjfc.3886","DOIUrl":"https://doi.org/10.47866/2615-9252/vjfc.3886","url":null,"abstract":"Honey is a natural product with a variety of uses. The antibacterial activity of honey is one of the key factors that make honey beneficial. The study aims to compare some quality index and antibacterial capacity of some main kinds of honey in Vietnam (determined by the diameters of the inhibition zones, the minimum inhibitory concentration - MIC, and the bactericidal concentrations - MBC). The results showed that the honey samples met the quality requirements of TCVN 12605:2019 for honey, including free reducing sugar content (ranging 67 - 72 g/100 g), diastase activity (ranging from 5.58 ± 0.46 to 25.27 ± 0.05 Schad), HMF (5-Hydroxymethylfurfural) content (ranging from 18.06 ± 0.09 to 23.80 ± 0.25 mg/kg). All honey samples exhibited antibacterial activity against all eight strains of bacteria studied as follows: the diameters of the inhibition zones ranged from 8.50 ± 0.29 to 22.33 ± 1.53 (mm), MIC ranged from 0.15 ± 0.00 to 0.90 ± 0.14 (g/mL). Honey from coffee and rubber flowers showed strong bactericidal ability at MBC concentration from 0.25 g/mL.","PeriodicalId":12896,"journal":{"name":"Heavy metals and arsenic concentrations in water, agricultural soil, and rice in Ngan Son district, Bac Kan province, Vietnam","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79151388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：