Industrial Crops and Products最新文献_第9页

Functional reshaping of rhizosphere microorganisms drives the degradation of soil multifunctionality in continuously cultivated Rhodiola crenulata 根际微生物功能重塑驱动连作红景天土壤多功能性退化

IF 6.2 1区农林科学 Q1 AGRICULTURAL ENGINEERING

Industrial Crops and Products

Pub Date : 2026-02-05 DOI: 10.1016/j.indcrop.2026.122837

Hong-Mei Jia , Xue-Mei Wu , Hui-Yang Deng , Guo-Peng Chen , Rong Ding , Rui Gu

Continuous cultivation is a common mode in the production of medicinal plants. Rhodiola crenulata, as a rare and endangered medicinal plant, like other medicinal plants, suffers from a decline in soil functionality due to long-term monoculture, which may constrain its growth and yield. However, the microbial community succession, soil functional changes, and their relationship during the artificial cultivation of R. crenulata remain poorly understood. In this study, rhizosphere soils from R. crenulata cultivated for 1-4 years were analyzed. By combining physical and chemical properties, extracellular enzyme activity analysis, high-throughput sequencing, and functional prediction, the relationship between microorganisms and soil under continuous cultivation was explored. The results showed that continuous cultivation was associated with a marked decline in soil multifunctionality, which decreased by approximately 46.19 % from the first to the fourth year, accompanied by significant reductions in available nutrients and microbial biomass carbon (77.81 %) and nitrogen (41.91 %). In contrast, activities of peroxidase (POD) and polyphenol oxidase (PPO) increased significantly with cultivation duration. The fungal community shifted from saprophytic to a coexistence of pathogenic and symbiotic types, with an enrichment of potential pathogenic fungi in the Hypocreales and Eurotiales. The carbon and nitrogen cycling functions of the bacterial community declined, whereas sulfur cycling and stress response functions increased. Concurrently, the co-occurrence network revealed intensified microbial competition and decreased functional redundancy. These findings suggest that the reshaping of microbial functions under continuous cultivation is a key driver of the decline in soil multifunctionality.

连作栽培是药用植物生产中常用的一种方式。红景天（Rhodiola crenulata）作为一种珍稀濒危药用植物，与其他药用植物一样，由于长期单一栽培，土壤功能下降，可能制约其生长和产量。然而，人工栽培过程中微生物群落演替、土壤功能变化及其相互关系尚不清楚。本研究对栽培1 ~ 4年的木犀草根际土壤进行了分析。通过结合理化性质、胞外酶活性分析、高通量测序和功能预测等方法，探讨了连作条件下微生物与土壤的关系。结果表明，连作土壤多功能性显著下降，第1 ~第4年土壤多功能性下降约46.19 %，速效养分和微生物生物量碳（77.81 %）、氮（41.91 %）显著减少。随着培养时间的延长，过氧化物酶（POD）和多酚氧化酶（PPO）活性显著升高。真菌群落由腐生型向致病型和共生型共存型转变，潜在致病真菌在下creale和eurotiale中富集。细菌群落的碳、氮循环功能下降，而硫循环和胁迫响应功能增加。同时，共生网络表明微生物竞争加剧，功能冗余减少。这些发现表明，在连续栽培下，微生物功能的重塑是土壤多功能性下降的关键驱动因素。

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引用次数: 0

A single-base deletion in ZjSGR confers the STAY-GREEN phenotype and the enhanced stress tolerance in Zoysia japonica under dark and low temperature stresses ZjSGR基因的单碱基缺失使结缕草的STAY-GREEN表型和在黑暗和低温胁迫下的抗逆性增强

IF 5.9 1区农林科学 Q1 AGRICULTURAL ENGINEERING

Industrial Crops and Products

Pub Date : 2026-02-05 DOI: 10.1016/j.indcrop.2026.122836

Jiahang Zhang, Lijing Li, Zhiwei Zhang, Yueyue Liu, Jiajing Mao, Jinchao Li, Na Zhang, Liebao Han, Lixin Xu

Zoysia japonica (Zoysia japonica Steud.) is a perennial warm-season grass widely used for soil and water conservation due to its extensive root system and resilience in lands, which has a huge potential in enhancing carbon sinks, restoring degraded ecosystems, and improving the living environment. However, Z. japonica will lose color and go into withering prematurely in late fall and winter compared with cool-season grass in the transition zone and the northern regions, which limits the application of Z. japonica. In this study, we successfully cultivated a “G” single-base deletion mutant of ZjSGR (STAY-GREEN) in Z. japonica (sgr-mutant) by CRISPR/Cas9 transient expression without exogenous genes. Based on the experiments of the sgr-mutant and the wild-type plant (WT-plant) under dark and low temperature stresses, the study demonstrates that CRISPR/Cas9-mediated novel single-base deletion of ZjSGR in Z. japonica exhibits not only prolonged chlorophyll retention but also superior stress tolerance under both dark and low temperature stress treatments. The mechanism of the improved stress tolerance of the sgr-mutant includes the accumulation of chlorophyll and carotenoid, providing a foundational buffer against oxidative damage, and a flexible regulation of key antioxidant enzymes in an energy-efficient way with a lower transcriptional burden compared to the WT-plant. Therefore, our study demonstrates that SGR plays a vital role in both leaf color and stress tolerance in grass, providing a new breeding strategy for developing green-persistence, stress-tolerance and low-maintenance grass applications.

结缕草（Zoysia japonica Steud.）是一种多年生暖季草，因其根系广泛，在土地上具有弹性，被广泛用于水土保持，在增强碳汇、恢复退化生态系统、改善生存环境方面具有巨大潜力。然而,z粳稻将失去颜色和进入过早枯萎在深秋和冬天相比,冷季草在过渡区和北部地区,这限制了z .粳稻的应用。在本研究中，我们通过CRISPR/Cas9无外源基因瞬时表达的方法，成功培养了ZjSGR （STAY-GREEN）在Z. japonica （sgr-mutant）中的“G”单碱基缺失突变体。通过对sgr突变体和野生型植物（WT-plant）在黑暗和低温胁迫下的实验，研究表明，CRISPR/ cas9介导的ZjSGR新型单碱基缺失不仅在黑暗和低温胁迫下表现出较长的叶绿素保留时间，而且具有较强的抗逆性。sgr-突变体提高抗逆性的机制包括叶绿素和类胡萝卜素的积累，提供抗氧化损伤的基础缓冲，以及关键抗氧化酶的高效灵活调节，与wt -植物相比，转录负担较低。因此，我们的研究表明，SGR在草的叶片颜色和抗逆性中都起着至关重要的作用，为开发绿色持久性、抗逆性和低维护性的牧草提供了新的育种策略。

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引用次数: 0

Unraveling the regulatory mechanisms of ZnO nanoparticles in mitigating salt stress toxicity in cotton 揭示氧化锌纳米颗粒减轻棉花盐胁迫毒性的调控机制

IF 6.2 1区农林科学 Q1 AGRICULTURAL ENGINEERING

Industrial Crops and Products

Pub Date : 2026-02-05 DOI: 10.1016/j.indcrop.2026.122844

Yuling Guo , Le Liu , Mengli Yu , Rumeng Zhao , Bowei Xu , Jiajie Yang , Zhongxian Li , Gang Wang , Liqiang Fan , Zuoren Yang

Soil salinization is an escalating environmental challenge that severely impairs cotton productivity. Although ZnO nanoparticles (ZNPs) have shown promise in enhancing plant stress tolerance, their regulatory mechanisms in mitigating salt stress toxicity in cotton remain poorly understood. This study revealed the regulatory mechanism by which ZNPs significantly mitigate salt stress toxicity in cotton by integrating physiological, metabolic, and transcriptional responses. The synthesized ZNPs (hydrodynamic diameter: 28.3 ± 5.3 nm; ζ-potential: −41.5 ± 7.8 mV) partially restored plant height, leaf area, and chlorophyll content, improving photosynthetic performance under salt stress. Furthermore, ZNPs treatment enhanced antioxidant enzyme activities (SOD, POD, CAT, APX) and rebalanced phytohormone homeostasis—ameliorating declines in GA₃ and IAA, and attenuating excessive ABA accumulation. Time-resolved metabolomics (3277 metabolites) and transcriptomics (72,127 genes) revealed that ZNPs mitigate salt-induced metabolic perturbation and reprogram early transcriptional dynamics (notably within 12–24 h), forming co-expression modules enriched in flavonoid biosynthesis, hormone signaling, fatty acid biosynthesis, and photosynthesis pathways. Integrative analysis identified GhFAB, a fatty acid biosynthesis gene induced by ZNPs. Phenotypic assessments revealed that GhFAB-OE lines exhibited significantly enhanced salt tolerance, with increased chlorophyll content, plant height, and fresh weight under NaCl treatment, whereas its suppression increased salt sensitivity, validating its functional role, demonstrate that GhFAB acts as a positive regulator in ZNPs-mediated salt tolerance in cotton. Our findings provide a multi-dimensional understanding of ZNPs-mediated salt tolerance and offer a strategic framework for developing nanoparticle-based agronomic practices to improve crop tolerance in saline environments.

土壤盐碱化是一个日益严重的环境挑战，严重损害了棉花的生产力。虽然氧化锌纳米颗粒（ZNPs）在提高植物抗逆性方面表现出了良好的前景，但它们在减轻棉花盐胁迫毒性方面的调控机制尚不清楚。本研究揭示了ZNPs通过综合生理、代谢和转录反应显著减轻棉花盐胁迫毒性的调控机制。合成的ZNPs（水动力直径：28.3 ± 5.3 nm； ζ-电位：−41.5 ± 7.8 mV）部分恢复了植株高度、叶面积和叶绿素含量，提高了盐胁迫下的光合性能。此外，ZNPs处理提高了抗氧化酶（SOD， POD， CAT， APX）活性，重新平衡了植物激素稳态，改善了GA₃和IAA的下降，并减弱了过量的ABA积累。时间分辨代谢组学（3277个代谢物）和转录组学（72,127个基因）显示，ZNPs减轻了盐诱导的代谢扰动，并重新编程了早期转录动力学（特别是在12-24 h内），形成了富含类黄酮生物合成、激素信号、脂肪酸生物合成和光合作用途径的共表达模块。整合分析鉴定出由ZNPs诱导的脂肪酸生物合成基因GhFAB。表型分析显示，在NaCl处理下，GhFAB- oe品系表现出显著增强的耐盐性，叶绿素含量、株高和鲜重均有所增加，而对其抑制则增加了盐敏感性，验证了其功能作用，表明GhFAB在znps介导的棉花耐盐性中起着正调节作用。我们的研究结果提供了对znps介导的耐盐性的多维理解，并为开发基于纳米颗粒的农艺实践提供了战略框架，以提高作物在盐环境中的耐受性。

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引用次数: 0

Successive grafting reprograms vegetative phase transition in black locust (Robinia pseudoacacia L.), a woody crop species 连续嫁接改变了木本作物刺槐（Robinia pseudoacacia L.）的营养相变

IF 6.2 1区农林科学 Q1 AGRICULTURAL ENGINEERING

Industrial Crops and Products

Pub Date : 2026-02-04 DOI: 10.1016/j.indcrop.2026.122821

Yapeng Li , Juan Han , Yizhe Cheng , Hao Yang , Jie Liu , Houyin Deng , Zijie Zhang , Yuhan Sun , Yousry A. El-Kassaby , Chao Lu , Ruihua Wu , Yun Li , Ye Zhao

Rejuvenation of mature woody plants is critical for restoring regenerative capacity and improving clonal propagation efficiency, yet effective induction strategies and their regulatory mechanisms remain poorly understood. This study aimed to determine whether successive grafting onto juvenile root-cutting rootstocks can induce systemic rejuvenation in mature black locust and to elucidate the underlying physiological and molecular mechanisms. Apical scions collected from mature trees were subjected to two successive grafting cycles onto juvenile root-cutting rootstocks, generating first- and second-generation grafted seedlings, which were compared with mature trees and juvenile root-cutting plantlets. Morphological, anatomical, physiological, transcriptomic, small RNA, and global DNA methylation analyses were conducted to evaluate rejuvenation-related changes. Successive grafting progressively restored juvenile traits, including increased leaf area, enhanced trichome density, reduced leaf thickness, and elongation of spines. Physiological analyses revealed decreased protein, phenolic, and malondialdehyde contents, together with a marked increase in net photosynthetic rate. Molecular analyses showed significant upregulation of microRNA156, repression of microRNA172, and a pronounced reduction in global DNA methylation levels during rejuvenation. Weighted gene co-expression network analysis identified RpMYC2 as a central transcriptional hub whose expression increased across grafting generations and was accompanied by elevated endogenous jasmonic acid accumulation and coordinated induction of jasmonate-related genes. Heterologous overexpression of RpMYC2 in Arabidopsis thaliana delayed flowering and suppressed AtFT expression, indicating a conserved role in age-related developmental regulation. These results demonstrate that successive grafting onto juvenile root-cutting rootstocks is an effective strategy for inducing rejuvenation in mature black locust and identify RpMYC2 as a key regulator linking jasmonate signaling to developmental phase reversal.

成熟木本植物的再生是恢复再生能力和提高无性系繁殖效率的关键，但有效的诱导策略及其调控机制尚不清楚。本研究旨在确定嫁接到幼龄切根砧木上是否能诱导成熟刺槐的全身返老还老，并阐明潜在的生理和分子机制。将成熟树木的顶端接穗连续两次嫁接到幼树扦插砧木上，形成第一代和第二代嫁接苗，并与成熟树木和幼树扦插苗进行比较。通过形态学、解剖学、生理学、转录组学、小RNA和整体DNA甲基化分析来评估返老还童相关的变化。连续嫁接逐渐恢复幼枝性状，包括叶面积增加、毛状体密度增加、叶厚度减少和刺伸长。生理分析显示蛋白质、酚类物质和丙二醛含量降低，净光合速率显著增加。分子分析显示，在年轻化过程中，microRNA156显著上调，microRNA172受到抑制，整体DNA甲基化水平显著降低。加权基因共表达网络分析发现，RpMYC2是一个中心转录枢纽，其表达在嫁接代间增加，并伴随着内源茉莉酸积累的增加和茉莉酸相关基因的协调诱导。RpMYC2在拟南芥中的异源过表达延迟开花，抑制AtFT的表达，表明其在年龄相关的发育调控中具有保守作用。这些结果表明，连续嫁接到幼根砧木上是诱导成熟刺槐返老还老的有效策略，并确定了RpMYC2是茉莉酸信号通路与发育阶段逆转的关键调控因子。

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引用次数: 0

Spatiotemporal soil canopy full-profile sensing reveals light–water–yield coupling in cotton, facilitating precise resource management in staggered-sowing fields 时空土壤冠层全剖面遥感揭示棉花光水产量耦合，为错播田资源精准管理提供依据

IF 6.2 1区农林科学 Q1 AGRICULTURAL ENGINEERING

Industrial Crops and Products

Pub Date : 2026-02-04 DOI: 10.1016/j.indcrop.2026.122832

Simeng Guo , Fengqi Wu , Lu Feng , Yaping Lei , Shiwu Xiong , Yahui Jiao , Xiaoyu Zhi , Beifang Yang , Yingchun Han , Yabing Li

Coordinating the efficient use of multiple natural resources is critical for ensuring sustainable crop production, particularly under increasingly variable climate conditions. The light interception rate (LIR) and soil water consumption (SWC) are fundamental to increasing cotton productivity, yet studies on their coupled dynamics across the soil–canopy continuum remain scarce. We established a spatial-grid, multisensor network that can be used to continuously monitor the LIR and SWC along with full soil-canopy profiles (-110–110 cm) in a staggered‑sowing cotton experiment in China. Geostatistics and nonlinear models were employed to quantify the spatiotemporal changes in LIR and SWC and their links to biomass and yield. Sowing date substantially affected SWC patterns, which exhibited clear seasonal and vertical stratification characteristics. Compared with the early-sown treatments, the late-sown treatments consistently demonstrated lower consumption of shallow water (10–40 cm) but higher consumption of deep water (70–110 cm). Early sowing advanced the upper-canopy LIR peaks by 10–12 days, whereas late sowing sustained high interception (>90 %) for a longer period during peak flowering. SWC at each position along the soil profile was significantly positively correlated with the canopy LIR, highlighting the spatial coordination between above- and belowground resource capture. In normal years, the SWC at shallow positions was positively correlated with seed cotton yield, with correlations turning negative below 40 cm and being greatest at 90 cm in cotton rows, indicating an optimal zone (10–40 cm) for water extraction that supports productivity. However, this relationship may be disturbed by extreme weather. This study revealed critical spatiotemporal interactions across the soil profile and crop canopy, offering a new technical paradigm for spatiotemporally coupled soil–crop monitoring and providing a functional basis for optimizing cotton production in data-driven agricultural systems to guide precision management, yield optimization, and climate-adaptive decision-making in future smart agriculture.

协调多种自然资源的有效利用对于确保可持续作物生产至关重要，特别是在气候条件日益变化的情况下。光截留率（LIR）和土壤耗水量（SWC）是提高棉花生产力的基础，但对它们在土壤-冠层连续体上的耦合动态研究仍然很少。在中国的交错播种棉花试验中，我们建立了一个空间网格、多传感器网络，可用于连续监测LIR和SWC以及全土壤-冠层剖面（-110-110 cm）。采用地统计学和非线性模型定量分析了LIR和SWC的时空变化及其与生物量和产量的关系。播期显著影响SWC分布，表现出明显的季节和垂直分层特征。与早播处理相比，晚播处理均表现出较低的浅水耗用（10 ~ 40 cm）和较高的深水耗用（70 ~ 110 cm）。早播可使上冠层LIR峰值提前10-12天，而晚播可在花期较长时间内保持较高的截留率（> 90% %）。土壤剖面各位置的SWC与冠层LIR呈显著正相关，凸显了地上和地下资源捕获的空间协调性。在正常年份，浅层SWC与籽棉产量呈正相关，在40 cm以下为负相关，在棉花行90 cm处相关性最大，表明10-40 cm是支持产量的最佳抽水量区域。然而，这种关系可能会受到极端天气的干扰。该研究揭示了土壤剖面与作物冠层之间的关键时空相互作用，为土壤-作物时空耦合监测提供了新的技术范式，并为数据驱动农业系统中的棉花生产优化提供了功能基础，以指导未来智慧农业的精准管理、产量优化和气候适应性决策。

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引用次数: 0

Strategies for vine shoots valorization using hydrothermal treatment and delignification using deep eutectic solvents within a biorefinery scheme 在生物精炼方案中使用水热处理和使用深共晶溶剂脱木质素的藤芽增值策略

IF 5.9 1区农林科学 Q1 AGRICULTURAL ENGINEERING

Industrial Crops and Products

Pub Date : 2026-02-04 DOI: 10.1016/j.indcrop.2026.122822

Alexandre Rubira, Pablo G. del Río, Meirielly Jesus, Beatriz Gullón, Aloia Romaní

Vine shoots (VS) are one of the main residues generated by winemaking, usually disposed of by open-field burning, which contributes to environmental pollution. Owing to their high content of lignin, cellulose and hemicelluloses, these residues represent a valuable feedstock for the bio-based products production. This study assessed environmentally friendly pretreatment strategies based on deep eutectic solvents (DES) and water to enable the valorization of vine shoots within a biorefinery framework. Two processing routes were compared: direct DES delignification and a sequential configuration combining autohydrolysis followed by DES delignification. The eutectic mixture of choline chloride and formic acid (ChCl:FA) at 130 °C for 60 min was identified as the most suitable condition for full valorization of vine shoots. Direct DES delignification yielded a delignified VS with low lignin content (5.41 kg of lignin per100 kg VS) and glucan recovery yield of 68 %. In contrast, the sequential strategy enabled the recovery of a hemicelluloses-rich aqueous stream containing 43.8 g/L of oligosaccharides. Although glucan retention in the solid was slightly lower, 25 % of glucan was additionally recovered as glucooligosaccharides and 55 % of lignin was removed. Overall, the combined autohydrolysis-DES approach proved to be more suitable for an integrated biorefinery, allowing milder conditions, ∼50 % delignification efficiency, and multiproduct recovery including cellulose-rich solids, hemicellulosic oligosaccharides, and high-purity lignin.

葡萄枝是酿酒过程中产生的主要残余物之一，通常采用露天焚烧的方式处理，造成环境污染。由于其木质素、纤维素和半纤维素的高含量，这些残留物代表了生物基产品生产的宝贵原料。本研究评估了基于深度共晶溶剂（DES）和水的环保型预处理策略，以使藤芽在生物炼制框架内增值。比较了两种处理路线：直接DES脱木质素和顺序配置结合自水解随后DES脱木质素。经鉴定，在130℃、60 min条件下，氯化胆碱与甲酸共晶混合物（ChCl:FA）是葡萄幼苗充分增殖的最适宜条件。直接DES脱木质素产生低木质素含量的脱木质素VS（5.41 kg每100 kg VS）和葡聚糖回收率为68 %。相比之下，顺序策略能够回收含有43.8 g/L低聚糖的富含半纤维素的水溶液。虽然葡聚糖在固体中的保留率略低，但25 %的葡聚糖被回收为低聚糖，55 %的木质素被去除。总的来说，结合自水解- des方法被证明更适合于集成生物精炼厂，允许更温和的条件，~ 50% %的脱木质素效率，以及多产品回收，包括富含纤维素的固体，半纤维素低聚糖和高纯度木质素。

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引用次数: 0

Development of bio-based polyurethane composite adhesives derived from castor oil and epoxidized soybean oil polyols: Influence of fumed silica and chain extender on strength, stability and wettability 蓖麻油和环氧大豆油多元醇生物基聚氨酯复合胶粘剂的研制：气相二氧化硅和扩链剂对强度、稳定性和润湿性的影响

IF 5.9 1区农林科学 Q1 AGRICULTURAL ENGINEERING

Industrial Crops and Products

Pub Date : 2026-02-04 DOI: 10.1016/j.indcrop.2026.122798

Mahsa Ghasemlou, Mir Saeed Seyed Dorraji, Fatemeh Dodangeh, Hamid Reza Ashjari

引用次数: 0

Nitrated lignin-reinforced polyvinyl alcohol films with enhanced optical and mechanical properties 具有增强光学和机械性能的硝化木质素增强聚乙烯醇薄膜

IF 5.9 1区农林科学 Q1 AGRICULTURAL ENGINEERING

Industrial Crops and Products

Pub Date : 2026-02-04 DOI: 10.1016/j.indcrop.2026.122816

Aliaksandr Karnitski, Sung-Soo Kim

引用次数: 0

Integrated metabolomics and sensoryomics reveal scent diversification of 20 iris species for fragrance-targeted breeding 综合代谢组学和感觉组学揭示了20种鸢尾花的气味多样性，为香味定向育种提供了依据

IF 5.9 1区农林科学 Q1 AGRICULTURAL ENGINEERING

Industrial Crops and Products

Pub Date : 2026-02-04 DOI: 10.1016/j.indcrop.2026.122760

Junming Sun, Jinxue Du, Yameng Lv, Yingxi Zhao, Yafei Niu, Lu Jing, Kexin Tian, Chuhan Zhi, Qian Lou, Ying Zhu, Haiying Chen, Hongwu Chen

Iris is worldwide acknowledged as a fragrant flower and source of perfume, but the chemical composition and sensory characteristics of their fragrance remain poorly understood. This study analyzed the volatile compounds of 20 iris species, and investigated the volatile compounds of two iris species (Iris. halophila pall. var. sogdiana (Bung) Grubov and I. uniflora Pall. ex Link) in particular in various flowering stages and flower organs using headspace solid-phase microextraction (HS-SPME) combined with gas chromatography-mass spectrometry (GC-MS). Meanwhile, sensory evaluation was conducted for the scent preferences, intensity, and types of the 20 species. The HS-SPME-GC-MS showed that fatty acid derivatives and terpenoid dominated the volatile compounds; and key volatile compounds identified included hexanal, (E)-2-hexanal, 3-hexanal, 2-methyl-4-pentenal, 2-hexenal, linalool, methyl heptenone, oxime-methoxy-phenyl-, β-ocimene, ethyl caprate, and nonanal. Sensory evaluation confirmed that all the 20 iris species exhibited fragrance, and diverse composition of scent types. The combination of sensory evaluation and relative odor contribution (ROC) analysis indicated that 16 out of the 20 iris species have been identified with characteristic scent compounds. During the flowering period of the two specifically investigated species, the emission of volatile compounds initially increased and then decreased, peaking at the full bloom stage. For the emission of volatile compounds, the falls displayed the highest, followed by the standards and pistils. In this emission process, the osmophore along the petal margins played a significant role. The stamens emitted the least volatile compounds with a special composition. These findings provide valuable insights for the breeding of fragrant iris and the utilization of wild iris resources.

鸢尾花是世界公认的芳香花卉和香水的来源，但其香味的化学成分和感官特征仍然知之甚少。本研究对20种鸢尾植物挥发物成分进行了分析，并对2种鸢尾植物的挥发物成分进行了研究。halophila笼罩。变种粟特草（Bung） Grubov和I. uniflora Pall。利用顶空固相微萃取（HS-SPME）和气相色谱-质谱（GC-MS）相结合的方法对不同花期和花器官进行了分析。同时，对20种昆虫的气味偏好、强度和类型进行了感官评价。HS-SPME-GC-MS分析表明，挥发性化合物以脂肪酸衍生物和萜类化合物为主；鉴定出的主要挥发性化合物包括己醛、(E)-2-己醛、3-己醛、2-甲基-4-戊烯醛、2-己烯醛、芳樟醇、甲基庚烯酮、肟-甲氧基苯基、β-辛烯、己酸乙酯和壬醛。感官评价结果表明，20种鸢尾花均呈现香味，且香气类型组成多样。感官评价和相对气味贡献（relative odor contribution， ROC）分析表明，20种鸢尾中有16种鉴定出了特征气味化合物。在两种植物的花期，挥发性化合物的释放量先增加后减少，在盛花期达到峰值。对于挥发性化合物的排放，瀑布最高，其次是标准和雌蕊。在这一排放过程中，花瓣边缘的渗透层发挥了重要作用。雄蕊散发出挥发性最低的特殊成分。这些发现对野生鸢尾资源的开发利用和香鸢尾的选育具有重要的指导意义。

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引用次数: 0

TgWRKY25-TgSBP1 module promotes cell expansion in Torreya grandis cones TgWRKY25-TgSBP1模块促进香榧球果细胞扩增

IF 6.2 1区农林科学 Q1 AGRICULTURAL ENGINEERING

Industrial Crops and Products

Pub Date : 2026-02-04 DOI: 10.1016/j.indcrop.2026.122834

Xinyu Li , Zhihui Liu , Ya Liu , Jiawen Yan , Jinwei Suo , Weiwu Yu , Jiasheng Wu , Jingwei Yan

Sucrose-binding proteins (SBPs), a class of proteins in plants, are involved in sucrose transport and utilization, and play a vital contribution in regulating plant growth and development. As demonstrated by prior studies, in plants such as soybean and tobacco, SBP-mediated sucrose transport is crucial for both plant growth and development and carbohydrate partitioning. However, in comparison to the extensive research on the function of SBP in angiosperms, there is currently a lack of in-depth studies on the specific role of SBPs in gymnosperms. In the gymnosperm Torreya grandis, we identified that TgSBP1 may be involved in the cell expansion of its cones by mediating sucrose accumulation. In this study, we found that during cone development in T. grandis, the expression of TgSBP1 showed a significant upward trend. Heterologous expression in tomatoes and transient overexpression in T. grandis cones indicated that TgSBP1 positively modulated the expansion of cone cells. In contrast, transient silencing of TgSBP1 significantly diminished sucrose accumulation and reduced cell size. Furthermore, the WRKY transcription factor TgWRKY25 was identified to participate in regulating TgSBP1 expression. Further functional analysis revealed that TgWRKY25 directly bound to the promoter of TgSBP1 and upregulate its expression, which increased sucrose content in cones and led to a significant enlargement of cell size. Taken together, these findings indicate that TgWRKY25 modulates sucrose level by upregulating TgSBP1 expression, thereby promoting cone cell expansion in T. grandis.

蔗糖结合蛋白（sbp）是植物体内参与蔗糖转运和利用的一类蛋白质，在调节植物生长发育中起着重要作用。先前的研究表明，在大豆和烟草等植物中，sbp介导的蔗糖转运对植物生长发育和碳水化合物分配都至关重要。然而，相对于对被子植物收缩压功能的广泛研究，目前对裸子植物收缩压的具体作用还缺乏深入的研究。在裸子植物大香榧中，我们发现TgSBP1可能通过介导蔗糖积累参与其球果的细胞扩增。在本研究中，我们发现在大田鼠的球果发育过程中，TgSBP1的表达呈明显上升趋势。TgSBP1在番茄中的异源表达和在大田葵球果中的瞬时过表达表明，TgSBP1正调控了球果细胞的扩增。相比之下，TgSBP1的短暂沉默显著减少了蔗糖积累和细胞大小的减小。此外，我们还发现WRKY转录因子TgWRKY25参与调控TgSBP1的表达。进一步的功能分析表明，TgWRKY25直接结合TgSBP1的启动子并上调其表达，使球果中蔗糖含量增加，导致细胞大小显著增大。综上所述，这些发现表明TgWRKY25通过上调TgSBP1的表达来调节蔗糖水平，从而促进大田鼠锥体细胞的扩增。

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引用次数: 0