Polysaccharides serve as promising ingredients for health-beneficial functional foods, while there were no investigations into the structural characterizations and bioactivities of an edible mushroom Cantharellus yunnanensis. In the study, crude polysaccharides from this mushroom were extracted by hot water and isolated by ethanol precipitation. Then, a neutral polysaccharide (named CY-1) was purified from the crude polysaccharide by deproteinization with Sevag reagent, decolorization with a kind of macroporous adsorption resin SP-825, DEAE-52 cellulose column chromatography and dialysis. The physicochemical properties of CY-1 were characterized by UV, IR, SEM, NMR, and HPLC analyses. Structural characterizations revealed that CY-1 is a homogeneous heteropolysaccharide with an average molecular weight of 3.06 × 104 Da. CY-1 exhibited a honeycomb structure with an irregular branching shape, and it was composed of mannose, glucose, fucose, xylose, arabinose, galactose, rhamnose, and glucuronic acid, with molar fractions of 54.83%, 25.11%, 10.28% 4.53%, 2.12%, 1.64%, 0.83%, and 0.64%, respectively. In vitro hypoglycemic tests showed that CY-1 had an inhibitory effect on α-glucosidase. In addition, CY-1 of 160 µg/mL promoted glucose consumption in normal HepG2 cells. These results suggested that CY-1 may be a potential hypoglycemic agent.
{"title":"Extraction, Characterization, and In Vitro Hypoglycemic Activity of a Neutral Polysaccharide from the New Medicinal Mushroom Cantharellus yunnanensis (Agaricomycetes).","authors":"Zhang-Chao Pan, Yu-Zhuo Zhang, Zhi-Qun Liang, Yong Wang, Nian-Kai Zeng","doi":"10.1615/IntJMedMushrooms.2023049072","DOIUrl":"https://doi.org/10.1615/IntJMedMushrooms.2023049072","url":null,"abstract":"<p><p>Polysaccharides serve as promising ingredients for health-beneficial functional foods, while there were no investigations into the structural characterizations and bioactivities of an edible mushroom Cantharellus yunnanensis. In the study, crude polysaccharides from this mushroom were extracted by hot water and isolated by ethanol precipitation. Then, a neutral polysaccharide (named CY-1) was purified from the crude polysaccharide by deproteinization with Sevag reagent, decolorization with a kind of macroporous adsorption resin SP-825, DEAE-52 cellulose column chromatography and dialysis. The physicochemical properties of CY-1 were characterized by UV, IR, SEM, NMR, and HPLC analyses. Structural characterizations revealed that CY-1 is a homogeneous heteropolysaccharide with an average molecular weight of 3.06 × 104 Da. CY-1 exhibited a honeycomb structure with an irregular branching shape, and it was composed of mannose, glucose, fucose, xylose, arabinose, galactose, rhamnose, and glucuronic acid, with molar fractions of 54.83%, 25.11%, 10.28% 4.53%, 2.12%, 1.64%, 0.83%, and 0.64%, respectively. In vitro hypoglycemic tests showed that CY-1 had an inhibitory effect on α-glucosidase. In addition, CY-1 of 160 µg/mL promoted glucose consumption in normal HepG2 cells. These results suggested that CY-1 may be a potential hypoglycemic agent.</p>","PeriodicalId":14025,"journal":{"name":"International journal of medicinal mushrooms","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9969052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.1615/IntJMedMushrooms.2023048522
Somanjana Khatua, Krishnendu Acharya
Macrocybe lobayensis owe popularity in several traditional cultures not only for delectable taste but also for its nutraceutical profile conveying great health benefits. Previous investigations have enumerated several bioactivities of the valuable mushroom such as antioxidant, anti-ageing, immune-modulation, and anti-tumor properties where polysaccharides played the key role. Macrofungi polymers are generally isolated by the conventional hot water process discarding the residue which still contains plenty of therapeutic components. The present study thus aimed to re-use such leftover of the edible macrofungus by immersing it in NaOH solution at high temperature supporting circular economy. Consequently a polysaccharide fraction, namely ML-HAP, was isolated that was found to be consisted of a homogenous heteropolysaccharide with molecular weight of ~ 128 kDa and β-glucan as the chief ingredient as evident by spectroscopy, gel-permeation chromatography, high performance thin-layer chromatography, and Fourier transform infrared. Antioxidant activity assays revealed that the macromolecules possess good radical scavenging, metal ion binding and reducing power. Nevertheless, strong immune-potentiation was also recorded as the extract triggered murine macrophage cell viability, pinocytosis, nitric oxide production and morpho-dynamics within 24 h where the best effect was executed at the level of 100 µg/mL. Altogether, the polysaccharides extracted from M. lobayensis exhibited a potent application prospect in functional food, pharmaceutical, nutraceutical and health care industries that could raise economic value of the underexplored mushroom.
{"title":"Chemical Characterization and Biological Functions of Hot Alkali-Soluble Crude Polysaccharide from the Water-Insoluble Residue of Macrocybe lobayensis (Agaricomycetes) Fruit Bodies.","authors":"Somanjana Khatua, Krishnendu Acharya","doi":"10.1615/IntJMedMushrooms.2023048522","DOIUrl":"https://doi.org/10.1615/IntJMedMushrooms.2023048522","url":null,"abstract":"<p><p>Macrocybe lobayensis owe popularity in several traditional cultures not only for delectable taste but also for its nutraceutical profile conveying great health benefits. Previous investigations have enumerated several bioactivities of the valuable mushroom such as antioxidant, anti-ageing, immune-modulation, and anti-tumor properties where polysaccharides played the key role. Macrofungi polymers are generally isolated by the conventional hot water process discarding the residue which still contains plenty of therapeutic components. The present study thus aimed to re-use such leftover of the edible macrofungus by immersing it in NaOH solution at high temperature supporting circular economy. Consequently a polysaccharide fraction, namely ML-HAP, was isolated that was found to be consisted of a homogenous heteropolysaccharide with molecular weight of ~ 128 kDa and β-glucan as the chief ingredient as evident by spectroscopy, gel-permeation chromatography, high performance thin-layer chromatography, and Fourier transform infrared. Antioxidant activity assays revealed that the macromolecules possess good radical scavenging, metal ion binding and reducing power. Nevertheless, strong immune-potentiation was also recorded as the extract triggered murine macrophage cell viability, pinocytosis, nitric oxide production and morpho-dynamics within 24 h where the best effect was executed at the level of 100 µg/mL. Altogether, the polysaccharides extracted from M. lobayensis exhibited a potent application prospect in functional food, pharmaceutical, nutraceutical and health care industries that could raise economic value of the underexplored mushroom.</p>","PeriodicalId":14025,"journal":{"name":"International journal of medicinal mushrooms","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10010649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Strain aging has been mainly contributing to the "uncertainty" of Morchella farming. The situation calls for urgent quantitative assessment of strain aging in cultivated Morchella mushrooms. In this paper, systemic senescence of the productive strains of M. eximia, M. importuna, and M. sextelata was achieved through successive subculturing to provide subcultures with different degree of aging for further studies. Then the quantitative assessment of morel strain aging was conducted by activity assay of amylase and xylanase using dinitrosalicylic acid (DNS) method. The results suggested that both activity of amylase and xylanase decreased along with the rise of subculture times. Meanwhile, the correlation between xylanase activity and time of subculturing in the tested morel strains was higher than that of amylase assay. Consequently, assay of amylase and xylanase activity by DNS method can be used in the quantitative assessment of morel strain aging, and assay of xylanase activity is the better alternative. The work will improve the settlement of "uncertainty" in the morel industry and thus be beneficial for stable development of morel farming.
{"title":"Activity Assay of Amylase and Xylanase Is Available for Quantitative Assessment of Strain Aging in Cultivated Culinary-Medicinal Morchella Mushrooms (Ascomycotina).","authors":"Peixin He, Zhuo Chen, Ying Men, Miaomiao Wang, Wensheng Wang, Wei Liu","doi":"10.1615/IntJMedMushrooms.2022046420","DOIUrl":"https://doi.org/10.1615/IntJMedMushrooms.2022046420","url":null,"abstract":"<p><p>Strain aging has been mainly contributing to the \"uncertainty\" of Morchella farming. The situation calls for urgent quantitative assessment of strain aging in cultivated Morchella mushrooms. In this paper, systemic senescence of the productive strains of M. eximia, M. importuna, and M. sextelata was achieved through successive subculturing to provide subcultures with different degree of aging for further studies. Then the quantitative assessment of morel strain aging was conducted by activity assay of amylase and xylanase using dinitrosalicylic acid (DNS) method. The results suggested that both activity of amylase and xylanase decreased along with the rise of subculture times. Meanwhile, the correlation between xylanase activity and time of subculturing in the tested morel strains was higher than that of amylase assay. Consequently, assay of amylase and xylanase activity by DNS method can be used in the quantitative assessment of morel strain aging, and assay of xylanase activity is the better alternative. The work will improve the settlement of \"uncertainty\" in the morel industry and thus be beneficial for stable development of morel farming.</p>","PeriodicalId":14025,"journal":{"name":"International journal of medicinal mushrooms","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9228680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.1615/IntJMedMushrooms.2023047505
Muniba Shafique, Abdul Rehman Niazi, Muhammad Imran, Muhammad Zaheer
The present study was an attempt to evaluate the antimicrobial and anthelmintic potential of two Amanita species, i.e., A. orsonii and A. glarea, along with their qualitative mycochemical screening. Maceration technique was adopted to make crude extracts in non-polar (petroleum ether and chloroform) and polar (ethanol and distilled water) solvents. Qualitative mycochemical screening revealed the presence of a variety of secondary metabolites like terpenoids, flavonoids, tannins, alkaloids, saponins, and cardiac glycosides. Antimicrobial activities were carried out by using agar well diffusing method against four bacterial and one fungal strain. The antibacterial potential ranged between 4.86 ± 0.088 mm and 34.83 ± 0.166 mm with maximum inhibition zone exhibited by petroleum ether extract against Pseudomonas fluorescens and least potential by distilled water macerate of A. orsonii against the Escherichia coli. The antifungal activity ranged between 14.5 ± 0.288 and 24.76 ± 0.145 mm, with the highest potential provided by chloroform extract of A. orsonii and least capability put forward by A. glarea by petroleum ether extract against Fusarium solanii. Antibiotics and antimycotic discs were used as standard and some of the crude extracts showed bigger zone of inhibition as compared to standard. Different concentrations of ethanolic extracts of both mushrooms were subjected to anthelmintic potential against parasite Haemonchus contortus. All the crude extracts were more potent than standard oxfandazole used. Anthelmintic potential ranged between 9.9 ± 0.057 and 54.93 ± 0.033 minutes for death of parasite, while the paralyzed time ranged between 4.86 ± 0.088 and 24.86 ± 0.088 minutes. From the results obtained it was concluded that both mushrooms can be used as potential source of curative antibacterial, antifungal, and anthelmintic agents against several diseases that might be used in pharmaceutical industries for making medicines and to screen out secondary metabolites in future.
{"title":"Extrapolating Qualitative Mycochemical Profiling and Therapeutic Potential of Two Mycorrhizal Amanita Species (Agaricomycetes) from Pakistan.","authors":"Muniba Shafique, Abdul Rehman Niazi, Muhammad Imran, Muhammad Zaheer","doi":"10.1615/IntJMedMushrooms.2023047505","DOIUrl":"https://doi.org/10.1615/IntJMedMushrooms.2023047505","url":null,"abstract":"<p><p>The present study was an attempt to evaluate the antimicrobial and anthelmintic potential of two Amanita species, i.e., A. orsonii and A. glarea, along with their qualitative mycochemical screening. Maceration technique was adopted to make crude extracts in non-polar (petroleum ether and chloroform) and polar (ethanol and distilled water) solvents. Qualitative mycochemical screening revealed the presence of a variety of secondary metabolites like terpenoids, flavonoids, tannins, alkaloids, saponins, and cardiac glycosides. Antimicrobial activities were carried out by using agar well diffusing method against four bacterial and one fungal strain. The antibacterial potential ranged between 4.86 ± 0.088 mm and 34.83 ± 0.166 mm with maximum inhibition zone exhibited by petroleum ether extract against Pseudomonas fluorescens and least potential by distilled water macerate of A. orsonii against the Escherichia coli. The antifungal activity ranged between 14.5 ± 0.288 and 24.76 ± 0.145 mm, with the highest potential provided by chloroform extract of A. orsonii and least capability put forward by A. glarea by petroleum ether extract against Fusarium solanii. Antibiotics and antimycotic discs were used as standard and some of the crude extracts showed bigger zone of inhibition as compared to standard. Different concentrations of ethanolic extracts of both mushrooms were subjected to anthelmintic potential against parasite Haemonchus contortus. All the crude extracts were more potent than standard oxfandazole used. Anthelmintic potential ranged between 9.9 ± 0.057 and 54.93 ± 0.033 minutes for death of parasite, while the paralyzed time ranged between 4.86 ± 0.088 and 24.86 ± 0.088 minutes. From the results obtained it was concluded that both mushrooms can be used as potential source of curative antibacterial, antifungal, and anthelmintic agents against several diseases that might be used in pharmaceutical industries for making medicines and to screen out secondary metabolites in future.</p>","PeriodicalId":14025,"journal":{"name":"International journal of medicinal mushrooms","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9421631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.1615/IntJMedMushrooms.2023047581
Zhiyin Tao, Qianwang Zheng
Pleurotus citrinopileatus, also known as golden oyster mushroom, is a newly industrialized edible mushroom mainly distributed in East Asia. It is a kind of saprophytic edible fungus with strong degradation characteristics, commonly found on fallen trees and stumps of broad-leaf tree species. So far, abundant kinds of bioactive compounds such as polysaccharides, ergothioneine, sesquiterpenes, and glycoprotein have been isolated from P. citrinopileatus and studied. Studies have confirmed that these compounds are beneficial to human health. In this paper, the recent studies on the cultivation, degradation characteristics application, and health effects of P. citrinopileatus are reviewed, and their development trends are discussed.
{"title":"Examining the Cultivation, Degradation Characteristics and Health Effects of the Golden Oyster Mushroom Pleurotus citrinopileatus (Agaricomycetes): A Review.","authors":"Zhiyin Tao, Qianwang Zheng","doi":"10.1615/IntJMedMushrooms.2023047581","DOIUrl":"https://doi.org/10.1615/IntJMedMushrooms.2023047581","url":null,"abstract":"<p><p>Pleurotus citrinopileatus, also known as golden oyster mushroom, is a newly industrialized edible mushroom mainly distributed in East Asia. It is a kind of saprophytic edible fungus with strong degradation characteristics, commonly found on fallen trees and stumps of broad-leaf tree species. So far, abundant kinds of bioactive compounds such as polysaccharides, ergothioneine, sesquiterpenes, and glycoprotein have been isolated from P. citrinopileatus and studied. Studies have confirmed that these compounds are beneficial to human health. In this paper, the recent studies on the cultivation, degradation characteristics application, and health effects of P. citrinopileatus are reviewed, and their development trends are discussed.</p>","PeriodicalId":14025,"journal":{"name":"International journal of medicinal mushrooms","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9475171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.1615/IntJMedMushrooms.2023047671
Nevena Petrovic, Marijana Kosanic, Tomislav Tosti, Ivana Srbljak, Ana Đurić
Honey mushroom, Armillaria mellea, is an edible and medicinal lignicolous basidiomycete. In this study, we investigated the chemical composition and bioactive properties of its methanolic and acetonic extracts. The chemical characterization of extracts was done with the HPLC-DAD-MS/MS method. The results showed that potassium was the most abundant mineral; chlorogenic acid was the most abundant polyphenol; malic acid was the most abundant organic acid; and among carbohydrates, the most abundant were sorbitol, glucose, fructose, and saccharose. Antioxidative activity was assessed by DPPH (IC50 of the methanolic extract was 608.32 μg/mL and of the acetonic extract 595.71 μg/mL) and reducing power assays (the results ranged between 0.034 and 0.102 μg/mL). Total phenolic content was determined as gallic acid equivalent (methanolic extract: 4.74 mg GAE/g; acetonic extract: 5.68 mg GAE/g). The microdilution assay was used to evaluate the antimicrobial activity of the extracts, and the results ranged from 1.25 to 20 mg/mL. The antidiabetic effect of the extracts was tested by the α-amylase (the results ranged from 34.90% to 41.98%) and α-glucosidase assays (the results were in the range of 0.55-2.79%). The neuroprotective activity was explored by the acetylcholinesterase inhibition assay (the results were in the range of 1.94-7.76%). The microtetrazolium assay was used to investigate the cytotoxic activity of the extracts (the IC50 values ranged from 212.06 to > 400 μg/mL). Although some results suggest that some activities of the extracts are relatively moderate, the honey mushroom can still be considered an excellent source of food and bioactive compounds with medicinal value.
{"title":"Chemical Characterization and Bioactive Properties of the Edible and Medicinal Honey Mushroom Armillaria mellea (Agaricomycetes) from Serbia.","authors":"Nevena Petrovic, Marijana Kosanic, Tomislav Tosti, Ivana Srbljak, Ana Đurić","doi":"10.1615/IntJMedMushrooms.2023047671","DOIUrl":"https://doi.org/10.1615/IntJMedMushrooms.2023047671","url":null,"abstract":"<p><p>Honey mushroom, Armillaria mellea, is an edible and medicinal lignicolous basidiomycete. In this study, we investigated the chemical composition and bioactive properties of its methanolic and acetonic extracts. The chemical characterization of extracts was done with the HPLC-DAD-MS/MS method. The results showed that potassium was the most abundant mineral; chlorogenic acid was the most abundant polyphenol; malic acid was the most abundant organic acid; and among carbohydrates, the most abundant were sorbitol, glucose, fructose, and saccharose. Antioxidative activity was assessed by DPPH (IC50 of the methanolic extract was 608.32 μg/mL and of the acetonic extract 595.71 μg/mL) and reducing power assays (the results ranged between 0.034 and 0.102 μg/mL). Total phenolic content was determined as gallic acid equivalent (methanolic extract: 4.74 mg GAE/g; acetonic extract: 5.68 mg GAE/g). The microdilution assay was used to evaluate the antimicrobial activity of the extracts, and the results ranged from 1.25 to 20 mg/mL. The antidiabetic effect of the extracts was tested by the α-amylase (the results ranged from 34.90% to 41.98%) and α-glucosidase assays (the results were in the range of 0.55-2.79%). The neuroprotective activity was explored by the acetylcholinesterase inhibition assay (the results were in the range of 1.94-7.76%). The microtetrazolium assay was used to investigate the cytotoxic activity of the extracts (the IC50 values ranged from 212.06 to > 400 μg/mL). Although some results suggest that some activities of the extracts are relatively moderate, the honey mushroom can still be considered an excellent source of food and bioactive compounds with medicinal value.</p>","PeriodicalId":14025,"journal":{"name":"International journal of medicinal mushrooms","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9421629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.1615/IntJMedMushrooms.2023047722
Li He, Fang Xie, Xia Ying Xia, Qiang Jun Su, Gang Zhou, Zhao He Chen, Cheng Gang Wang
The asexual form of Ophiocordyceps sinensis has been controversial, but various morphologic mycelium appeared when O. sinensis was cultured under experimental conditions. To explore the generation mechanism of morphologic mycelium, developmental transcriptomes were analyzed from three kinds of mycelium (aerial mycelium, hyphae knot, and substrate mycelium). The results showed that diameter and morphology of these three kinds of mycelium were obviously different. KEGG functional enrichment analysis showed that the differential expressed genes (DEGs) of substrate mycelium were enriched in ribosomes and peroxisomes, indicating that prophase culture was rich in nutrients and the metabolism of substrate mycelium cells was vigorous in the stage of nutrient absorption. The up-DEGs of hyphae knot were mainly enriched in the oxidative phosphorylation pathway, indicating that oxidative phosphorylation was the main energy source for mycelium formation in the stage of nutrient accumulation and reproductive transformation. The up-DEGs of aerial mycelium were mainly enriched in the synthesis and degradation pathways of valine, leucine, and isoleucine, suggesting that the occurrence of aerial mycelium was related to amino acid metabolism at the later stage of culture, and nutritional stress accelerated the reproduction of asexual spores. In addition, the important roles of mycelium formation related genes were verified by combined analysis of qRT-PCR and transcriptome sequencing. Collectively, this study will provide theoretical guidance for inhibiting the occurrence of aerogenous mycelium and promoting the development of mycelium into pinhead primordia in the culture of O. sinensis in the future.
{"title":"Uncovering Gene Expression Profiles of Three Morphologic Mycelium Forms of the Chinese Caterpillar Mushroom Ophiocordyceps sinensis (Ascomycota) Using High-Throughput Sequencing.","authors":"Li He, Fang Xie, Xia Ying Xia, Qiang Jun Su, Gang Zhou, Zhao He Chen, Cheng Gang Wang","doi":"10.1615/IntJMedMushrooms.2023047722","DOIUrl":"https://doi.org/10.1615/IntJMedMushrooms.2023047722","url":null,"abstract":"<p><p>The asexual form of Ophiocordyceps sinensis has been controversial, but various morphologic mycelium appeared when O. sinensis was cultured under experimental conditions. To explore the generation mechanism of morphologic mycelium, developmental transcriptomes were analyzed from three kinds of mycelium (aerial mycelium, hyphae knot, and substrate mycelium). The results showed that diameter and morphology of these three kinds of mycelium were obviously different. KEGG functional enrichment analysis showed that the differential expressed genes (DEGs) of substrate mycelium were enriched in ribosomes and peroxisomes, indicating that prophase culture was rich in nutrients and the metabolism of substrate mycelium cells was vigorous in the stage of nutrient absorption. The up-DEGs of hyphae knot were mainly enriched in the oxidative phosphorylation pathway, indicating that oxidative phosphorylation was the main energy source for mycelium formation in the stage of nutrient accumulation and reproductive transformation. The up-DEGs of aerial mycelium were mainly enriched in the synthesis and degradation pathways of valine, leucine, and isoleucine, suggesting that the occurrence of aerial mycelium was related to amino acid metabolism at the later stage of culture, and nutritional stress accelerated the reproduction of asexual spores. In addition, the important roles of mycelium formation related genes were verified by combined analysis of qRT-PCR and transcriptome sequencing. Collectively, this study will provide theoretical guidance for inhibiting the occurrence of aerogenous mycelium and promoting the development of mycelium into pinhead primordia in the culture of O. sinensis in the future.</p>","PeriodicalId":14025,"journal":{"name":"International journal of medicinal mushrooms","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9421633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.1615/IntJMedMushrooms.2022046684
Bao-Zhong Yuan, Jie Sun
This study analyzed 1,739 papers on medicinal mushrooms published from 1999 to July 18, 2022 based on Web of Science (WoS). Papers were mainly written in English (1,733, 99.655%), from 6,502 authors, 92 countries or territories, 1,862 organizations and published in 311 journals and 3 book series. International Journal of Medicinal Mushrooms published 1,069 (61.472%) papers. Top 5 countries or regions were P.R. China, India, Taiwan, USA, and Malaysia; each published more than 87 papers. From the average citations, papers from Ukraine, Israel, Netherlands, Serbia, and Thailand show the highest citations per paper (more than 22.9 times per paper). The top five affiliations were Chinese Academy of Sciences, University of Malaya, University of Haifa, National Chung Hsing University, and Shanghai Academy of Agricultural Sciences, each with more than 49 papers. Top five authors are Wasser SP, Hyde KD, Mau JL, Sabaratnam V, Yang Y; each published more than 26 papers. The paper with the most was Wasser SP in Applied Microbiology and Biotechnology (2002), which has 1442 citations and the average number of citations is 68.67 times per year. Based on the ESI database, there are 13 top papers with 13 highly cited papers and 1 hot paper. All keywords in medicinal mushrooms research were separated into ten clusters according to different research topics. The results will help researchers clarify the current situation and provide guidance for future research.
本研究分析了1999年至2022年7月18日在Web of Science (WoS)上发表的1739篇关于药用蘑菇的论文。论文以英文为主(1733篇,99.655%),作者6502人,来自92个国家或地区,1862个组织,发表于311种期刊和3个丛书。International Journal of Medicinal mushroom发表论文1069篇(61.472%)。排名前五的国家和地区分别是中国、印度、台湾、美国和马来西亚;每人发表论文87篇以上。从平均引用次数来看,乌克兰、以色列、荷兰、塞尔维亚、泰国等国家的论文被引用次数最多(超过22.9次)。排名前五的高校分别是中国科学院、马来亚大学、海法大学、国立中兴大学和上海农业科学院,每所高校发表论文均超过49篇。排名前五的作者分别是:Wasser SP、Hyde KD、Mau JL、Sabaratnam V、Yang Y;每人发表论文26篇以上。引用次数最多的论文是Wasser SP in Applied Microbiology and Biotechnology(2002),被引1442次,年平均被引68.67次。ESI数据库共收录顶级论文13篇,高被引论文13篇,热点论文1篇。根据不同的研究课题,将药菇研究中的关键词分为10个聚类。研究结果将有助于研究人员澄清现状,并为今后的研究提供指导。
{"title":"Visualization Analysis of Medicinal Mushrooms Research Topic Based on Web of Science.","authors":"Bao-Zhong Yuan, Jie Sun","doi":"10.1615/IntJMedMushrooms.2022046684","DOIUrl":"https://doi.org/10.1615/IntJMedMushrooms.2022046684","url":null,"abstract":"<p><p>This study analyzed 1,739 papers on medicinal mushrooms published from 1999 to July 18, 2022 based on Web of Science (WoS). Papers were mainly written in English (1,733, 99.655%), from 6,502 authors, 92 countries or territories, 1,862 organizations and published in 311 journals and 3 book series. International Journal of Medicinal Mushrooms published 1,069 (61.472%) papers. Top 5 countries or regions were P.R. China, India, Taiwan, USA, and Malaysia; each published more than 87 papers. From the average citations, papers from Ukraine, Israel, Netherlands, Serbia, and Thailand show the highest citations per paper (more than 22.9 times per paper). The top five affiliations were Chinese Academy of Sciences, University of Malaya, University of Haifa, National Chung Hsing University, and Shanghai Academy of Agricultural Sciences, each with more than 49 papers. Top five authors are Wasser SP, Hyde KD, Mau JL, Sabaratnam V, Yang Y; each published more than 26 papers. The paper with the most was Wasser SP in Applied Microbiology and Biotechnology (2002), which has 1442 citations and the average number of citations is 68.67 times per year. Based on the ESI database, there are 13 top papers with 13 highly cited papers and 1 hot paper. All keywords in medicinal mushrooms research were separated into ten clusters according to different research topics. The results will help researchers clarify the current situation and provide guidance for future research.</p>","PeriodicalId":14025,"journal":{"name":"International journal of medicinal mushrooms","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9228681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.1615/IntJMedMushrooms.2022047243
Tuan Manh Nguyen, Jaisoo Kim, Nguyen The Hung, Nguyen Huu Tho, Do Bich Due, Do Thi Hien
This study was conducted to evaluate extraction yield, antioxidant content, antioxidant capacity and antibacterial activity of extracts obtained from submerged mycelium (ME) and fruiting body (FBE) of Phellinus robiniae NTH-PR1. The results showed that yields of ME and FBE reached 14.84 ± 0.63 and 18.89 ± 0.86%, respectively. TPSC, TPC, and TFC were present in both mycelium and fruiting body, and the more contents of them were found in fruiting body. The concentrations of TPSC, TPC and TFC in ME and FBE were 17.61 ± 0.67 and 21.56 ± 0.89 mg GE g-1, 9.31 ± 0.45 and 12.14 ± 0.56 mg QAE g-1, and 8.91 ± 0.53 and 9.04 ± 0.74 mg QE g-1, respectively. EC50 values for DPPH radical scavenging revealed FBE (260.62 ± 3.33 μg mL-1) was more effective than ME (298.21 ± 3.61 μg mL-1). EC50 values for ferrous ion chelating in ME and FBE were 411.87 ± 7.27 and 432.39 ± 2.23 μg mL-1, respectively. Thus, both extracts were able to inhibit Gram-positive and Gram-negative pathogenic bacterial strains, at concentrations ranging in 25-100 mg mL-1 of ME and 18.75-75 mg mL-1 of FBE for Gram-positive bacteria; ranging in 75-100 mg mL-1 of ME and 50-75 of FBE for Gram-negative bacteria. Overall submerged mycelial biomass and fruiting bodies of Ph. robiniae NTH-PR1 can be considered as useful natural sources for development of functional food, pharmaceuticals and cosmetic products or cosmeceuticals.
{"title":"Antioxidant and Antimicrobial Properties of Fruiting Body and Submerged Mycelium of Medicinal Mushroom Phellinus robiniae (Agaricomycetes).","authors":"Tuan Manh Nguyen, Jaisoo Kim, Nguyen The Hung, Nguyen Huu Tho, Do Bich Due, Do Thi Hien","doi":"10.1615/IntJMedMushrooms.2022047243","DOIUrl":"https://doi.org/10.1615/IntJMedMushrooms.2022047243","url":null,"abstract":"<p><p>This study was conducted to evaluate extraction yield, antioxidant content, antioxidant capacity and antibacterial activity of extracts obtained from submerged mycelium (ME) and fruiting body (FBE) of Phellinus robiniae NTH-PR1. The results showed that yields of ME and FBE reached 14.84 ± 0.63 and 18.89 ± 0.86%, respectively. TPSC, TPC, and TFC were present in both mycelium and fruiting body, and the more contents of them were found in fruiting body. The concentrations of TPSC, TPC and TFC in ME and FBE were 17.61 ± 0.67 and 21.56 ± 0.89 mg GE g-1, 9.31 ± 0.45 and 12.14 ± 0.56 mg QAE g-1, and 8.91 ± 0.53 and 9.04 ± 0.74 mg QE g-1, respectively. EC50 values for DPPH radical scavenging revealed FBE (260.62 ± 3.33 μg mL-1) was more effective than ME (298.21 ± 3.61 μg mL-1). EC50 values for ferrous ion chelating in ME and FBE were 411.87 ± 7.27 and 432.39 ± 2.23 μg mL-1, respectively. Thus, both extracts were able to inhibit Gram-positive and Gram-negative pathogenic bacterial strains, at concentrations ranging in 25-100 mg mL-1 of ME and 18.75-75 mg mL-1 of FBE for Gram-positive bacteria; ranging in 75-100 mg mL-1 of ME and 50-75 of FBE for Gram-negative bacteria. Overall submerged mycelial biomass and fruiting bodies of Ph. robiniae NTH-PR1 can be considered as useful natural sources for development of functional food, pharmaceuticals and cosmetic products or cosmeceuticals.</p>","PeriodicalId":14025,"journal":{"name":"International journal of medicinal mushrooms","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9257110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.1615/IntJMedMushrooms.2022047219
Yoshiyuki Adachi, Fumiyasu Momose, Hiromi Momose, Rui Tada, Naohito Ohno
β-glucans are polysaccharides that activate innate immunity. We herein investigated whether P-glucans promote the immunological effects of antibody drugs against malignant tumor cells using human peripheral blood mononuclear cells (PBMCs). Rituximab bound to CD20-specific lymphoma and exhibited cytotoxic activity in the presence of human mononuclear cells, but not neutrophils. The addition of Sparassis crispa (cauliflower mushroom)-derived β-glucan (SCG) and granulocyte macrophage colony-stimulating factor (GM-CSF) to co-cultures of PBMCs and Raji lymphoma cells further promoted antibody-dependent cell-mediated cytotoxicity (ADCC). The GM-CSF treatment increased β-glucan receptor expression on adherent cells in PBMCs. A co-stimulation with GM-CSF and SCG of PBMCs induced an increase in the number of spreading cells and the activation of natural killer (NK) cells. The enhancement in ADCC was abolished by the removal of NK cells, indicating that SCG and GM-CSF increased ADCC against lymphoma by activating β-glucan receptor-expressing cells in PBMCs and enhancing NK cell activity. The synergistic mechanisms of action of mushroom-derived β-glucans and biopharmaceuticals, including recombinant cytokines and antibodies, in the treatment of malignant tumor cells provide important insights into the clinical efficacy of β-glucans from mushrooms.
{"title":"Potentiation of Antitumor Activity by Antibody Drugs and Mushroom-Derived β-Glucans in Natural Killer Cell-Mediated Tumoricidal Activities against Non-Hodgkin's B-Cell Lymphoma.","authors":"Yoshiyuki Adachi, Fumiyasu Momose, Hiromi Momose, Rui Tada, Naohito Ohno","doi":"10.1615/IntJMedMushrooms.2022047219","DOIUrl":"https://doi.org/10.1615/IntJMedMushrooms.2022047219","url":null,"abstract":"<p><p>β-glucans are polysaccharides that activate innate immunity. We herein investigated whether P-glucans promote the immunological effects of antibody drugs against malignant tumor cells using human peripheral blood mononuclear cells (PBMCs). Rituximab bound to CD20-specific lymphoma and exhibited cytotoxic activity in the presence of human mononuclear cells, but not neutrophils. The addition of Sparassis crispa (cauliflower mushroom)-derived β-glucan (SCG) and granulocyte macrophage colony-stimulating factor (GM-CSF) to co-cultures of PBMCs and Raji lymphoma cells further promoted antibody-dependent cell-mediated cytotoxicity (ADCC). The GM-CSF treatment increased β-glucan receptor expression on adherent cells in PBMCs. A co-stimulation with GM-CSF and SCG of PBMCs induced an increase in the number of spreading cells and the activation of natural killer (NK) cells. The enhancement in ADCC was abolished by the removal of NK cells, indicating that SCG and GM-CSF increased ADCC against lymphoma by activating β-glucan receptor-expressing cells in PBMCs and enhancing NK cell activity. The synergistic mechanisms of action of mushroom-derived β-glucans and biopharmaceuticals, including recombinant cytokines and antibodies, in the treatment of malignant tumor cells provide important insights into the clinical efficacy of β-glucans from mushrooms.</p>","PeriodicalId":14025,"journal":{"name":"International journal of medicinal mushrooms","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9257112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}