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Chemical composition of essential oils from Crocus ancyrensis (Herbert) Maw Spreading In Çorum (Türkiye) Region 在Çorum(土耳其)地区传播的番红花(Herbert)Maw精油的化学成分
Pub Date : 2023-06-16 DOI: 10.21448/ijsm.1199416
H. Doğan, Ömer Kayir, Erol Alver, İ. Bi̇li̇ci̇
Crocus ancyrensis is a yellow-flowered Crocus species and is in the same family and genus with saffron. Although various studies have been conducted on the antioxidant capacity and essential oil content characterization of saffron (Crocus sativus), there is no literature information about the C. ancyrensis plant, which is known as Crocus among the people. The members of Crocus family contain many valuable components including antioxidants, phenolic compounds and essential oils. The essential oils obtained from this family is a complex mixture of more than 30 components, which are primarily terpenes and their derivatives. These mixtures are used in paint, medicine, and food applications especially in the cosmetics sector. In the study, the essential oils of the Crocus ancyrensis plant were extracted with the clevenger system and characterized by GC-MS analyses. As a result, 23 volatile components were identified. 2-Hexenal, 1-ethylbutyl Hydroperoxide, 2-nitro-Hexane, β-Isophorone, α-Isophorone, 2-Caren-10-al and Eugenol are found as the main components of Crocus ancyrensis plant extract. Due to the antioxidant, antimicrobial, antifungal, anticancer and odorant properties of some of the identified components, C. ancyrensis can be used as a medicinal aromatic plant in various fields, especially in the cosmetics and perfume industry.
番红花是一种开黄色花的番红花,与番红花同属一科。尽管人们对藏红花(Crocus sativus)的抗氧化能力和精油含量特征进行了各种研究,但没有关于人们称之为番红花的C.ancyrensis植物的文献信息。番红花家族成员含有许多有价值的成分,包括抗氧化剂、酚类化合物和精油。从该家族获得的精油是由30多种成分组成的复杂混合物,这些成分主要是萜烯及其衍生物。这些混合物用于油漆、医药和食品应用,尤其是化妆品行业。本研究采用清除剂系统提取了番红花属植物的精油,并用气相色谱-质谱分析对其进行了表征。结果,鉴定出23种挥发性成分。2-己烯醛、1-乙基丁基过氧化氢、2-硝基己烷、β-异佛尔酮、α-异佛尔酮、2-Caren-10-al和Eugenol是番红花植物提取物的主要成分。由于一些已鉴定成分具有抗氧化、抗菌、抗真菌、抗癌和气味特性,C.ancyrensis可作为一种药用芳香植物应用于各个领域,尤其是在化妆品和香水行业。
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引用次数: 0
The effect of extraction methods on the yields, chemical composition and antifungal activity of sawdust Cedrus atlantica Manetti essential oils 研究了不同提取方法对木木屑精油产率、化学成分及抗真菌活性的影响
Pub Date : 2023-06-16 DOI: 10.21448/ijsm.1047122
Chaimaa Bouyahia, M. Benbouzi̇d, S. El hajjaji, M. Slaoui̇, F. Bentata, M. Labhi̇li̇li̇, A. Bouyahya
The aim of this work is to determine the effect of the extraction method on the yield, the chemical composition, and the antifungal activity of cedarwood essential oils (EOs) from sawdust of Moroccan Cedrus atlantica (C. atlantica). EOs were extracted by different methods: hydrodistillation, soxhlet, maceration, and ultrasound. The chemical composition was determined using gas chromatography mass spectrometry (GC-MS) analysis. The yields of EOs were 5.60%, 11.68%, 4.82% and 9.33% for hydrodistillation, soxhlet, maceration and ultrasound, respectively. GC-MS revealed a diversity of chemical compounds depending on extraction methods. Indeed, the main compound of EOs obtained by soxhlet, maceration, and ultrasound was copalic acid methyl ester by a rate of 28.41%, 20.24%, and 24.17%, respectively. However, -himachalene (21.32%) followed by -himachalene (9.40%), β-Copaen-4α-ol (7.71%) and longifolene (6.74%) are the main compounds of EO extracted by hydrodistillation. The antifungal activity of cedarwood EO was tested in vitro on two pathogenic fungi: Fusarium culmorum (F. Culmorum) and Botrytis cineria (B. Cinerea). The minimum inhibitory concentration (MIC) was determined by successive dilutions of the stock solutions. The extracted EOs by soxhlet, maceration and ultrasound showed the important inhibitory effect against B. cinerea (MIC=1.25 mL/L). However, F. culmorum showed resistance towards all tested EOs. The finding of this study showed clearly that the volatile composition of EOs can be variable according to extraction. methods. Moreover, antifungal effects are depending on chemical composition of EOs but also to tested staris.
本工作的目的是确定提取方法对摩洛哥大西洋雪松锯末中雪松精油的产量、化学成分和抗真菌活性的影响。EOs通过不同的方法提取:水溶、索氏、浸渍和超声。使用气相色谱-质谱(GC-MS)分析来测定化学成分。加氢蒸馏、索氏蒸馏、浸渍和超声的EOs产率分别为5.60%、11.68%、4.82%和9.33%。GC-MS揭示了不同提取方法的化合物的多样性。事实上,通过索氏法、浸渍法和超声法获得的EOs的主要化合物是共聚酸甲酯,其比率分别为28.41%、20.24%和24.17%。然而-himachalene(21.32%)-α-醇(7.71%)和长叶烯(6.74%)是EO的主要化合物。对雪松EO对两种病原真菌:镰刀菌(Fusarium culmorum)和灰葡萄孢(Botrytis cineria)的体外抗真菌活性进行了测试。最小抑制浓度(MIC)通过储备溶液的连续稀释来确定。经索氏、浸渍和超声提取的EOs对灰葡萄球菌具有重要的抑制作用(MIC=1.25mL/L)。然而,禾秆镰刀菌对所有测试的EOs都表现出抗性。这项研究的发现清楚地表明,EOs的挥发性成分可以根据提取而变化。方法。此外,抗真菌作用取决于EOs的化学成分,也取决于测试的淀粉。
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引用次数: 0
Ultrasonic assisted propolis extraction: characterization by ATR-FTIR and determination of its total antioxidant capacity and radical scavenging ability 超声波辅助蜂胶提取:用ATR-FTIR表征蜂胶提取工艺,测定其总抗氧化能力和自由基清除能力
Pub Date : 2023-06-16 DOI: 10.21448/ijsm.1167773
Ü. Erdoğan
In the current study, ultrasonic assisted ethanolic extract of propolis was discussed in detail, including their total phenolic content, total antioxidant capacity and radical scavenging capacity. For this purpose, we determined the total antioxidant capacity of propolis extract by CUPRAC and FRAP) assay. At the same time, the free radical scavenging capacity of propolis extracts was investigated via the DPPH• and CUPRAC- hydroxyl radical scavenging (HRS) methods. The chemical constituents of propolis extract were characterized by ATR-FTIR. The results revealed that propolis is rich in total phenolic components (189 mg GAE /g extract). According to the CUPRAC assay, the total antioxidant capacity of propolis extract was calculated to be 2.43 ± 0.07 mmol TE/g-propolis extract. FRAP value of propolis extract was determined as 1.11 mmol TE/g-propolis extract. DPPH• scavenging activity of propolis extract was calculated to be 0.71± 0.002 mmol TE / g - extract. On the other hand, according to the CUPRAC method, HRS capacity of propolis extract at different concentrations (5-10 μg/mL) was determined as 68.1% and 77.64 %, respectively. Research findings showed that propolis extract has a strong radical scavenging potential. The FTIR spectra of the functional groups originating from the phenolic compounds in the propolis extract were as expected.
本研究对超声波辅助蜂胶乙醇提取物进行了详细的研究,包括其总酚含量、总抗氧化能力和自由基清除能力。为此,我们通过CURAC和FRAP)测定法测定了蜂胶提取物的总抗氧化能力。同时,采用DPPH•和CUTRAC-羟基自由基清除(HRS)方法研究了蜂胶提取物对自由基的清除能力。用ATR-FTIR对蜂胶提取物的化学成分进行了表征。结果表明,蜂胶富含总酚类成分(189mg GAE/g提取物)。根据CURAC测定,蜂胶提取物的总抗氧化能力为2.43±0.07mmol TE/g-蜂胶提取物。蜂胶提取物的FRAP值为1.11mmol TE/g-蜂胶提取物。蜂胶提取物的DPPH•清除活性为0.71±0.002mmol TE/g提取物。另一方面,根据CURAC方法,不同浓度(5-10μg/mL)的蜂胶提取物的HRS容量分别为68.1%和77.64%。研究结果表明,蜂胶提取物具有较强的清除自由基的潜力。蜂胶提取物中源自酚类化合物的官能团的FTIR光谱如预期的那样。
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引用次数: 1
Investigation of antimicrobial and anticancer activity of extracts obtained following UV application to Althaea officinalis L. callus cultures 紫外光对杜鹃花愈伤组织培养物的抑菌和抗癌活性研究
Pub Date : 2023-06-13 DOI: 10.21448/ijsm.1291929
F. A. Özdemir, Mesut Turan
Althaea officinalis L., a plant of the Malvaceae family, is widely used in alternative medicine. The aim of this study is to cultivate the Althaea officinalis plant under in vitro conditions to create an appropriate callus regeneration protocol and investigate the antimicrobial and anticancer activities of methanol and ethyl acetate extracts of calli after UV-C application. Leaf, petiole, and root parts of A. officinalis plants germinated in a sterile environment were used as explant sources. Explants were cultured on MS medium containing different concentrations of 2,4-D (1, 2 mg/l) and BAP (0.25, 0.50, 0.75 mg/l). The most effective (100%) callus growth and callus weight (516.24±0.48 mg) was observed on petiole explants using MS medium containing 1 mg/l 2,4-D + 0.25 mg/l BAP. Calli obtained from leaf and petiole explants were exposed to UV-C treatment. Extractions of calli were carried out using methanol and ethyl acetate solutions. 1 mg/ml, 5 mg/ml, and 10 mg/ml solutions of methanol and ethyl acetate extracts were prepared and their antimicrobial activity on bacteria was investigated using the disc diffusion method for 7 different gram-positive and 9 different gram-negative bacteria. None of the three extract concentrations used had any antimicrobial activities. The anticancer activities of the extracts on SH-SY5Y human neuroblastoma cells were studied using the WST-1 viability kit. 1000, 500, 250, 125, and 62.5 µg/ml concentrations of ethyl acetate extracts of leaf and petiole calli had anticancer activity.
杜鹃花(Althaea officinalis L.)是一种锦葵科植物,被广泛用于替代医学。本研究的目的是在体外条件下培养officinalis植物,建立合适的愈伤组织再生方案,并研究UV-C处理后愈伤组织甲醇和乙酸乙酯提取物的抑菌和抗癌活性。以在无菌环境下萌发的铁皮石斛叶片、叶柄和根为外植体。外植体在含有不同浓度2,4- d (1,2 mg/l)和BAP(0.25、0.50、0.75 mg/l)的MS培养基上培养。在含有1 mg/l 2,4- d + 0.25 mg/l BAP的MS培养基上,叶柄外植体愈伤组织生长最显著(100%),愈伤组织质量最高(516.24±0.48 mg)。将叶片和叶柄外植体的愈伤组织暴露于UV-C处理下。愈伤组织采用甲醇和乙酸乙酯溶液进行提取。制备1 mg/ml、5 mg/ml和10 mg/ml的甲醇和乙酸乙酯提取物溶液,采用圆盘扩散法对7种革兰氏阳性菌和9种革兰氏阴性菌进行抑菌活性研究。三种提取物浓度均无抗菌活性。采用WST-1细胞活力检测试剂盒研究其提取物对SH-SY5Y人神经母细胞瘤细胞的抗肿瘤活性。1000、500、250、125和62.5µg/ml浓度的叶和叶柄愈伤组织乙酸乙酯提取物具有抗癌活性。
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引用次数: 0
Aroma profile of the essential oils from different parts of Pycnocycla aucherana Decne. ex Boiss. 圆叶青不同部位精油的香气特征。木香。
Pub Date : 2023-05-27 DOI: 10.21448/ijsm.1164925
Fatemeh ASKARİ, Fatemeh SEFİDKON, Zohreh EMAMİ BİSTGANİ, Mohamad Amin SOLTANİPOUR
The current study focused on the essential oil concentration and aroma profile in different parts of wild Pycnocycla aucherana Decne from Iran during two years 2017 and 2018. Plant samples of P. aucherana were collected from HajiAbad area located in Hormozgan province, Iran at reproductive stage on June 7, 2017 and 2018. Essential oil isolation was done by hydro-distillation method for 3 hours. For the qualification and quantification of components, gas chromatography/mass spectrometry (GC/MS) was applied. Results showed that the averages of essential oil percentage of shoot and leaf (SL) and seed in the first and second year were (0.13, 0.23%) and (0.4, 0.3%) respectively. The main chemotype was namely -phellandrene (5.96-16%), p-cymene (3.07-27.4%), Limonene (0.72-6.80%), γ-cadinene (0.8-4.33%), Spathulenol (1.90-8.64%), Elemol (0.3-6.69%), b-eudesmol (0.8-9.27), and Bulnesol (0.91-3.40%). The highest amount of -phellandrene (16%) and p-cymene (27.4%) was observed in the seed and (SL) of essential oils in the first year respectively. Elemol and Elemicin content increased during the second year in the seed of essential oils with amount of (6.69 %) and (25.69 %), respectively. Overall, the results showed that the geographic origin greatly influenced the chemical composition of P. aucherana.
目前的研究主要集中在2017年和2018年两年间,对来自伊朗的野生圆叶蝉(Pycnocycla aucherana Decne)不同部位的精油浓度和香气特征进行了研究。于2017年6月7日和2018年6月7日在伊朗霍尔木兹甘省HajiAbad地区采集了毛茛(P. aucherana)生殖期植物样本。采用水蒸气蒸馏法分离精油3小时。采用气相色谱/质谱法(GC/MS)对成分进行定性和定量分析。结果表明,茎叶挥发油含量(SL)和种子挥发油含量在第1年和第2年的平均值分别为(0.13,0.23%)和(0.4,0.3%)。主要化学型为-茶树烯(5.96-16%)、对伞花烯(3.07-27.4%)、柠檬烯(0.72-6.80%)、γ-cadinene(0.8-4.33%)、Spathulenol(1.90-8.64%)、Elemol(0.3-6.69%)、b-eudesmol(0.8-9.27)、Bulnesol(0.91-3.40%)。第1年,挥发油种子和挥发油中-费蓝烯含量最高(16%),对伞花烯含量最高(27.4%)。精油种子中Elemol和elicin含量在第二年分别增加了6.69%和25.69%。综上所述,地理来源对毛莨属植物的化学成分有较大影响。
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引用次数: 0
Antimicrobial evaluation of the Patchouli (Pogostemon cablin Benth.) leaf essential oil combination with standard antimicrobial compounds 广藿香(Pogostemon cablin Benth.)叶精油与标准抗菌化合物组合的抗菌评价
Pub Date : 2023-05-27 DOI: 10.21448/ijsm.1232606
Nazlı Şenay Beşi̇ri̇k, G. Göger
Pogostemon cablin Benth's leaves, which belong to the Lamiaceae family, are used to make patchouli essential oil (PEO). Sesquiterpenes are the main compounds in essential oil. Antibacterial, antifungal, antiviral, antiemetic, and anti-inflammatory activities are just a few of the many pharmacological actions that are well-known. In this investigation, cefuroxime (CEF), moxifloxacin (MOX), clarithromycin (CLA), fluconazole (FLU), and terbinafine (TER) were combined with patchouli (Pogostemon cablin leaf essential oil). Gram-positive Staphylococcus aureus ATCC 6538, Gram-negative Escherichia coli ATCC 8739, and yeasts Candida albicans ATCC 10231, and C. tropicalis ATCC 750 were tested against all combinations. Drug interaction was given as fractional inhibitory concentrations (FIC) after combinations of the essential oils were tested for their minimum inhibitory concentrations (MIC).The checkerboard method was used to assess antimicrobial interactions, Fluconazole, terbinafine, cefuroxime, and clarithromycin combined with essential oil demonstrated an "additive effect" against Candida strains together with E. coli and S. aureus.
广藿香的叶子属于Lamiaceae科,用于制作广藿香精油(PEO)。倍半萜是精油中的主要化合物。抗菌、抗真菌、抗病毒、止吐和抗炎活性只是众所周知的众多药理作用中的一小部分。在本研究中,头孢呋辛(CEF)、莫西沙星(MOX)、克拉霉素(CLA)、氟康唑(FLU)和特比萘芬(TER)与广藿香(广藿香叶精油)联合使用。针对所有组合测试革兰氏阳性金黄色葡萄球菌ATCC 6538、革兰氏阴性大肠杆菌ATCC 8739、白色念珠菌ATCC 10231和热带念珠菌ATCC 750。在测试精油组合的最低抑制浓度(MIC)后,以分数抑制浓度(FIC)给出药物相互作用。使用棋盘格法评估抗微生物相互作用,氟康唑、特比萘芬、头孢呋辛,克拉霉素与精油联合使用对念珠菌菌株、大肠杆菌和金黄色葡萄球菌具有“添加作用”。
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引用次数: 1
Investigation of In Vitro antiproliferative activity properties of Spartium junceum L. (Spanish broom) against MDA-MB-231 and HepG2 cancer cell lines 菝葜(Spartium junceum L.)体外抗MDA-MB-231和HepG2癌细胞增殖活性的研究
Pub Date : 2023-05-27 DOI: 10.21448/ijsm.1182965
Fatma Tuğçe GÜRAĞAÇ DERELİ, S. Akkoç
Cancer is among the top global public health burdens leading to millions of deaths each year. The study aims to investigate the antiproliferative effect of Spartium junceum L. flowers on different cancer cell lines. The ethanolic extract of the flowers was prepared in the present study. Phytochemical analysis of the plant extract revealed the presence of several phenolic compounds such as cinnamic acid and its derivatives (chlorogenic, p-coumaric, ferulic acids), protocatechuic acid, epicatechin and luteolin. This extract was tested against human breast (MDA-MB-231) and liver (HepG2) cancer cell lines to find out its antiproliferative activity. It was determined that the extract was effective against both cell lines with IC50 values of 2.37 ± 0.47 and 0.98 ± 0.01 µL/mL for MDA-MB-231 and HepG2, respectively. Particularly, the extract was found to be more effective in the liver cancer cell line than the breast cancer cell line. All these obtained findings led us to believe that this medicinal plant could be a promising antiproliferative agent candidate for the treatment of human liver and breast cancers.
癌症是全球最大的公共卫生负担之一,每年导致数百万人死亡。本研究旨在探讨米草(Spartium junceum L.)花对不同癌细胞系的抗增殖作用。本研究制备了红花的乙醇提取物。植物化学分析表明,该植物提取物中含有肉桂酸及其衍生物(绿原酸、对香豆酸、阿魏酸)、原儿茶酸、表儿茶素和木犀草素等酚类化合物。对人乳腺(MDA-MB-231)和肝脏(HepG2)癌细胞进行了抗增殖活性试验。结果表明,该提取物对MDA-MB-231和HepG2的IC50值分别为2.37±0.47和0.98±0.01µL/mL。特别是,提取物被发现对肝癌细胞系比乳腺癌细胞系更有效。这些发现使我们相信这种药用植物可能是治疗人类肝癌和乳腺癌的一种有前途的抗增殖药物。
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引用次数: 0
Synergistic antibacterial evaluation of Coriandri aetheroleum and linalool with standard antibiotics 芫荽油、芳樟醇与标准抗生素的协同抑菌效果评价
Pub Date : 2023-05-27 DOI: 10.21448/ijsm.1140803
Gözde Öztürk, G. Göger, Fatih Demirci, B. Demirci
Within this work, it was aimed to investigate the in vitro antibacterial properties of the Pharma grade coriander (Coriandrum sativum L.) essential oil, and its combinations. The chemical composition of the essential oil was confirmed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) analyses, simultaneously. Furthermore, the potential antibacterial activity of both the essential oil, and (+)-linalool with standard compounds chloramphenicol, and ciprofloxacin were evaluated using an in vitro microdilution assay against a panel of selected pathogens, namely Acinetobacter baumannii, Bacillus cereus, Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus. Initially, the minimum inhibitory concentrations (MIC) were determined, the essential oil as well as (+)-linalool and the standard antibiotics were combined for the synergistic antibacterial activity potential, where the combination activities were expressed as fractional inhibitory concentration index values (Σ FIC). The Coriander oil was relatively more effective against B. cereus, P. aeruginosa, E. coli, A. baumannii (2500 µg/mL) compared to S. aureus (5000 µg/mL), respectively. (+)- Linalool was found as effective as the essential oil (2500-5000 µg/mL). Coriander oil and antibiotic combinations showed synergistic effects against B. cereus (∑FIC= 0.375), E. coli (∑FIC= 0.078) and S. aureus (∑FIC= 0.375). Combination with (+)-linalool, and antibiotics showed synergistic effects against B. cereus (∑FIC= 0.375), E. coli (∑FIC= 0.093), as well as against S. aureus (∑FIC= 0.375), respectively. To the best of our knowledge, this is the first time of the antimicrobial combination study of linalool, coriander oil, chloramphenicol, and ciprofloxacin. The initial findings of this work suggest further natural product and drug combination evaluations.
在这项工作中,旨在研究药用级香菜精油及其组合的体外抗菌性能。同时通过气相色谱(GC)和气相色谱-质谱(GC-MS)分析确定了精油的化学成分。此外,使用体外微量稀释法评估了精油和(+)-芳樟醇与标准化合物氯霉素和环丙沙星对一组选定病原体的潜在抗菌活性,即鲍曼不动杆菌、蜡样芽孢杆菌、大肠杆菌、铜绿假单胞菌和金黄色葡萄球菌。最初,确定最小抑制浓度(MIC),将精油以及(+)-芳樟醇和标准抗生素组合以获得协同抗菌活性潜力,其中组合活性表示为部分抑制浓度指数值(∑FIC)。与金黄色葡萄球菌(5000µg/mL)相比,香菜油对蜡样芽孢杆菌、铜绿假单胞菌、大肠杆菌和鲍曼氏杆菌(2500µg/mL。(+)-芳樟醇被发现与精油(2500-5000µg/mL)一样有效。香菜油和抗生素组合对蜡样芽孢杆菌(∑FIC=0.375)、大肠杆菌(∑FIC=0.078)和金黄色葡萄球菌(∑FIC=0.075。据我们所知,这是首次对芳樟醇、香菜油、氯霉素和环丙沙星进行抗菌联合研究。这项工作的初步发现为进一步的天然产物和药物组合评估提供了建议。
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引用次数: 0
Phytochemical screening and in vitro antioxidant activities of Mentha suaveolens Ehrh. extract 薄荷的植物化学筛选及体外抗氧化活性。摘录
Pub Date : 2023-05-27 DOI: 10.21448/ijsm.1148664
Moha Afrokh, K. Boumhara, K. Chatoui̇, S. Tahrouch, A. Hati̇mi̇, H. Harhar, M. Tabyaoui
Within the framework of the valorization of the medicinal and aromatic plants of Morocco, we were interested during this study in the characterization and the phytochemical identification of some secondary metabolites present in Mentha suaveolens Ehrh. and the evaluation of the antioxidant activity of this species using four methods: DPPH free radical scavenging test, 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid, (ABTS) radical cation scavenging test, FRAP test measuring antioxidant power, and β bleaching-carotene. Mentha suaveolens is a species belonging to the Lamiaceae family, harvested in southern Morocco, and widely used in traditional medicine for its biological properties attributed mainly to phenolic compounds. Just as they protect plants, secondary metabolites also have a protective role in the human body and are therefore beneficial to our health. They are attributed, in particular, with antioxidant, anti-inflammatory, and antibacterial properties. In this regard, characterization and identification tests of secondary metabolites revealed the presence of alkaloids, flavonoids, catechic tannins, and terpenes in this plant, whereas, the aerial parts of this species are devoid of coumarins, cyanogenic compounds, saponins and free quinones. The quantification of the phenolic compounds gave high contents, with contents of total phenols (54.75 ± 5.62) μg GAE/mg, total flavonoids (32.41 ± 0.41) μg QE/mg, and total condensed tannins (27 ± 1) μg CE/mg. In addition, the results obtained show that Mentha suaveolens extract has stronger antioxidant activity using the β-Carotene method with IC50 (0.24 ± 0.06) mg/mL against IC50 (0.021 ± 0.001) mg/mL for the standard antioxidant by the DPPH free radical scavenging test.
在摩洛哥药用和芳香植物的定价框架内,我们在本研究中对薄荷中存在的一些次级代谢产物的表征和植物化学鉴定感兴趣。采用DPPH自由基清除试验、2,2'-叠氮双-(3-乙基苯并噻唑啉-6-磺酸、(ABTS)自由基阳离子清除试验、测定抗氧化能力的FRAP试验和β漂白胡萝卜素四种方法评价该物种的抗氧化活性。薄荷是Lamiaceae科的一种,原产于摩洛哥南部,因其主要由酚类化合物组成的生物特性而被广泛用于传统医学。次级代谢产物就像保护植物一样,对人体也有保护作用,因此对我们的健康有益。它们特别具有抗氧化、抗炎和抗菌的特性。在这方面,次级代谢产物的表征和鉴定测试表明,该植物中存在生物碱、类黄酮、儿茶素单宁和萜烯,而该物种的地上部分不含香豆素、发蓝化合物、皂苷和游离醌。酚类化合物的含量较高,总酚含量为(54.75±5.62)μgGAE/mg,总黄酮含量为(32.41±0.41)μgQE/mg,总缩合单宁含量为(27±1)μg CE/mg。此外,用β-胡萝卜素法测定薄荷提取物的抗氧化活性,其IC50为(0.24±0.06)mg/mL,用DPPH自由基清除试验测定其标准抗氧化物的IC50(0.021±0.001)mg/mL。
{"title":"Phytochemical screening and in vitro antioxidant activities of Mentha suaveolens Ehrh. extract","authors":"Moha Afrokh, K. Boumhara, K. Chatoui̇, S. Tahrouch, A. Hati̇mi̇, H. Harhar, M. Tabyaoui","doi":"10.21448/ijsm.1148664","DOIUrl":"https://doi.org/10.21448/ijsm.1148664","url":null,"abstract":"Within the framework of the valorization of the medicinal and aromatic plants of Morocco, we were interested during this study in the characterization and the phytochemical identification of some secondary metabolites present in Mentha suaveolens Ehrh. and the evaluation of the antioxidant activity of this species using four methods: DPPH free radical scavenging test, 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid, (ABTS) radical cation scavenging test, FRAP test measuring antioxidant power, and β bleaching-carotene. Mentha suaveolens is a species belonging to the Lamiaceae family, harvested in southern Morocco, and widely used in traditional medicine for its biological properties attributed mainly to phenolic compounds. Just as they protect plants, secondary metabolites also have a protective role in the human body and are therefore beneficial to our health. They are attributed, in particular, with antioxidant, anti-inflammatory, and antibacterial properties. In this regard, characterization and identification tests of secondary metabolites revealed the presence of alkaloids, flavonoids, catechic tannins, and terpenes in this plant, whereas, the aerial parts of this species are devoid of coumarins, cyanogenic compounds, saponins and free quinones. The quantification of the phenolic compounds gave high contents, with contents of total phenols (54.75 ± 5.62) μg GAE/mg, total flavonoids (32.41 ± 0.41) μg QE/mg, and total condensed tannins (27 ± 1) μg CE/mg. In addition, the results obtained show that Mentha suaveolens extract has stronger antioxidant activity using the β-Carotene method with IC50 (0.24 ± 0.06) mg/mL against IC50 (0.021 ± 0.001) mg/mL for the standard antioxidant by the DPPH free radical scavenging test.","PeriodicalId":14437,"journal":{"name":"International Journal of Secondary Metabolite","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43638174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
In-vitro anti-diabetic, anti-Alzheimer, anti-tyrosinase, antioxidant activities of selected coumarin and dihydroisocoumarin derivatives 香豆素和二氢异香豆素衍生物的体外抗糖尿病、抗阿尔茨海默病、抗酪氨酸酶、抗氧化活性
Pub Date : 2023-05-24 DOI: 10.21448/ijsm.1196712
H. Şahi̇n
Benzo-α-pyrone structured coumarin derivatives are secondary metabolites first obtained from Coumarouna odorata in 1822. Coumarin and its structural isomer dihydroisocoumarin derivatives are found in many different sources in nature. Several different bioactivities of these compounds have been reported. In this study, preliminary activity screening and comparison of four purchased coumarin derivatives (esculetin, esculin monohydrate, umbelliferon, scoparone) and four previously isolated 3-phenyl-3,4-dihydroisocoumarin derivatives (thunberginol C, scorzocreticoside I, scorzocreticoside II, scorzopygmaecoside) from a medicinal plant were carried out by in-vitro methods. α-Glucosidase, acetylcholinesterase, butyrylcholinesterase, tyrosinase inhibitor activities and antioxidant potentials of the compounds were evaluated. Consequently, thunberginol C (free – not glycosylated form of 3,4-dihydroisocoumarin structure) showed better potential in all enzyme inhibitory activities compared to coumarin structure. Particularly, α-glucosidase inhibitory activity of this compound with a very low IC50 value (94.76±2.98 µM) compared to standard acarbose (1036.2±2.70 µM) should be noted. Glycosylation and/or methoxy substitution of 3,4-dihydroisocoumarin structure resulted a significant decrease in all tested enzyme inhibitory activities. The structures of esculin MH, umbelliferone, scoparone, scorzocreticoside I, and scorzopygmaeceoside might be considered in further synthetic studies as selective acetylcholinesterase inhibitors. Thunberginol C has a promising potential in tyrosinase inhibitory activity. Esculetin and thunberginol C showed the best results with high potentials in antioxidant activity via 2,2-diphenyl-1-picryl-hydrazyl-hydrate free radical scavenging, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid cation radical decolorization, and cupric ion reducing antioxidant capacity assays compared to the standards.
苯并α-吡咯酮结构香豆素衍生物是1822年首次从香马ouna odorata中获得的次生代谢产物。香豆素及其结构异构体二氢异香豆素衍生物在自然界中有许多不同的来源。这些化合物的几种不同的生物活性已被报道。本研究采用体外筛选的方法,对从药用植物中获得的4种香豆素衍生物(蛇皮素、一水蛇皮素、伞花香豆素、天冬酮)和4种已分离的3-苯基-3,4-二氢异香豆素衍生物(雷牛蒡醇C、scorzocreticoside I、scorzocreticoside II、scorzopymaecoside)进行了初步的活性筛选和比较。对化合物的α-葡萄糖苷酶、乙酰胆碱酯酶、丁基胆碱酯酶、酪氨酸酶抑制活性和抗氧化活性进行了评价。因此,与香豆素结构相比,雷牛蒡醇C(3,4-二氢异香豆素结构的游离非糖基化形式)在所有酶抑制活性方面都表现出更好的潜力。值得注意的是,该化合物的α-葡萄糖苷酶抑制活性与标准阿卡波糖(1036.2±2.70µM)相比,IC50值(94.76±2.98µM)非常低(94.76±2.98µM)。3,4-二氢异香豆素结构的糖基化和/或甲氧基取代导致所有测试酶的抑制活性显著降低。esculin MH、伞花酮、天冬酮、scorzcreticcoside I和scorzopygmaeceoside的结构可能在进一步的合成研究中被认为是选择性乙酰胆碱酯酶抑制剂。在抑制酪氨酸酶活性方面,雷牛蒡醇C具有广阔的应用前景。通过对2,2-二苯基-1-吡啶-水合肼自由基的清除、2,2 ' -氮基-双(3-乙基苯并噻唑-6-磺酸阳离子自由基的脱色和铜离子还原抗氧化能力的测定,槲皮素和雷牛蒡醇C的抗氧化活性最高,具有较高的电位。
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引用次数: 1
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International Journal of Secondary Metabolite
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