The aim of the present study was to evaluate the marginal and internal fit of zirconium fixed partial denture using different impression techniques.
Methods
A Nissin Typodont model, including maxillary central incisor and canine with missing lateral incisor, was selected for the present study. Thirty zirconium frameworks were fabricated following three impression techniques (N = 10), conventional silicone impression (group C), scanned dental impression (group S), and Trios 3 (3Shape) intraoral scanner (group T). An extra-fine milling strategy was applied.
Results
Group T had the smallest discrepancy compared to groups C (P =0.006) and S (P =0.052) at the marginal level, whereas it was larger at the incisal tip. Discrepancies in group T were smaller than group C (P =0.004) when measured at the axial walls, and smaller than group S (P =0.045) when measured at the chamfer area for the central teeth only. Samples in group T showed a greater percentage of equally extended restorations (52.5%), while other groups were mainly underextended (group C: 63.7%, group S: 68.8%).
Conclusion
Better adaptation was achieved with the intraoral scanner group, except at the incisal tip. Conventional and scanned impressions revealed a greater percentage of underextended restorations.
{"title":"Evaluation of the fit of zirconia three-unit fixed partial dentures fabricated by different impression techniques","authors":"Ghassan Moustapha, Khaled Azzam, Emad AlShwaimi, Munir Silwadi, Marco Ferrari, Ziad Salameh","doi":"10.1111/jicd.12413","DOIUrl":"10.1111/jicd.12413","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>The aim of the present study was to evaluate the marginal and internal fit of zirconium fixed partial denture using different impression techniques.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>A Nissin Typodont model, including maxillary central incisor and canine with missing lateral incisor, was selected for the present study. Thirty zirconium frameworks were fabricated following three impression techniques (N = 10), conventional silicone impression (group C), scanned dental impression (group S), and Trios 3 (3Shape) intraoral scanner (group T). An extra-fine milling strategy was applied.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Group T had the smallest discrepancy compared to groups C (<i>P </i>=<i> </i>0.006) and S (<i>P </i>=<i> </i>0.052) at the marginal level, whereas it was larger at the incisal tip. Discrepancies in group T were smaller than group C (<i>P </i>=<i> </i>0.004) when measured at the axial walls, and smaller than group S (<i>P </i>=<i> </i>0.045) when measured at the chamfer area for the central teeth only. Samples in group T showed a greater percentage of equally extended restorations (52.5%), while other groups were mainly underextended (group C: 63.7%, group S: 68.8%).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Better adaptation was achieved with the intraoral scanner group, except at the incisal tip. Conventional and scanned impressions revealed a greater percentage of underextended restorations<b>.</b></p>\u0000 </section>\u0000 </div>","PeriodicalId":16204,"journal":{"name":"Journal of Investigative and Clinical Dentistry","volume":"10 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/jicd.12413","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37165914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The purpose of the present study was to consider racial differences across three survey questions related to adult oral health in the National Oral Health Surveillance System (NOHSS) between 1999 and 2014.
Methods
The NOHSS tracks population-level progress made toward improved oral health in the USA. NOHSS adult indicators of oral health data were extracted for 1999-2014, and trends were studied by race.
Results
Among adults ≥18 years in 1999, 70.7% of whites and 60.3% of blacks had visited a dentist in the past year. By 2014, 68.4% of whites and 56.5% of blacks had visited a dentist in the past year. Among adults aged ≥65 years in 1999, 24.5% of whites and 33.2% of blacks had lost all natural teeth due to dental caries or gingival/periodontal disease. By 2014, 14.3% of whites and 22.1% of blacks had lost all natural teeth.
Conclusions
There have been overall gains in key indicators of oral health in the USA; however, blacks remain far behind whites in the NOHSS adult oral health indicators.
{"title":"Fifteen-year gap between oral health of blacks and whites in the USA","authors":"Romesh P. Nalliah, Vladyslav Virun, Gurmukh Dhaliwal, Harleen Kaur, Anuradha Kote","doi":"10.1111/jicd.12415","DOIUrl":"10.1111/jicd.12415","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>The purpose of the present study was to consider racial differences across three survey questions related to adult oral health in the National Oral Health Surveillance System (NOHSS) between 1999 and 2014.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>The NOHSS tracks population-level progress made toward improved oral health in the USA. NOHSS adult indicators of oral health data were extracted for 1999-2014, and trends were studied by race.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Among adults ≥18 years in 1999, 70.7% of whites and 60.3% of blacks had visited a dentist in the past year. By 2014, 68.4% of whites and 56.5% of blacks had visited a dentist in the past year. Among adults aged ≥65 years in 1999, 24.5% of whites and 33.2% of blacks had lost all natural teeth due to dental caries or gingival/periodontal disease. By 2014, 14.3% of whites and 22.1% of blacks had lost all natural teeth.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>There have been overall gains in key indicators of oral health in the USA; however, blacks remain far behind whites in the NOHSS adult oral health indicators.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16204,"journal":{"name":"Journal of Investigative and Clinical Dentistry","volume":"10 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/jicd.12415","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37147649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The aims of the present study were to determine education inequalities in chronic periodontitis (CP) among Sri Lankan men and whether oral health behaviors explain education inequalities in CP.
Methods
Data from 720 males who participated in a study to determine the prevalence of CP in 30-60-year-olds in Colombo district, Sri Lanka, were used for the present study. An interviewer administered a questionnaire obtained information about sociodemographics and oral health behaviors. Following the assessment of periodontal parameters, case definitions proposed by the Centers for Disease Control/American Academy of Periodontology were used to define periodontitis.
Results
Education gradients were observed in relation to CP, smoking, betel quid chewing, alcohol use, and dental utilization. Education gradients in CP remained, but attenuated after adjustments for smoking, betel quid chewing, alcohol use, and dental utilization. Current smoking and current alcohol use explained 4%-38% and 6%-15% of the associations between education and CP, respectively. The education gradient in CP remained following simultaneous adjustment for all behaviors, but lost significance for 11-13 years of education.
Conclusion
Of the oral health behaviors considered, current smoking contributed the most to education inequalities in CP, explaining 4%-38% of the education differences in CP.
{"title":"Role of oral health-related behaviors in education inequalities in chronic periodontitis among Sri Lankan men","authors":"Nimali Wellappuli, Lilani Ekanayake","doi":"10.1111/jicd.12416","DOIUrl":"10.1111/jicd.12416","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aims</h3>\u0000 \u0000 <p>The aims of the present study were to determine education inequalities in chronic periodontitis (CP) among Sri Lankan men and whether oral health behaviors explain education inequalities in CP.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Data from 720 males who participated in a study to determine the prevalence of CP in 30-60-year-olds in Colombo district, Sri Lanka, were used for the present study. An interviewer administered a questionnaire obtained information about sociodemographics and oral health behaviors. Following the assessment of periodontal parameters, case definitions proposed by the Centers for Disease Control/American Academy of Periodontology were used to define periodontitis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Education gradients were observed in relation to CP, smoking, betel quid chewing, alcohol use, and dental utilization. Education gradients in CP remained, but attenuated after adjustments for smoking, betel quid chewing, alcohol use, and dental utilization. Current smoking and current alcohol use explained 4%-38% and 6%-15% of the associations between education and CP, respectively. The education gradient in CP remained following simultaneous adjustment for all behaviors, but lost significance for 11-13 years of education.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Of the oral health behaviors considered, current smoking contributed the most to education inequalities in CP, explaining 4%-38% of the education differences in CP.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16204,"journal":{"name":"Journal of Investigative and Clinical Dentistry","volume":"10 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/jicd.12416","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37302245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Renan Dal-Fabbro, Melyna Marques de Almeida, Leopoldo Cosme-Silva, Antonio H. C. Neto, Leda M. P. Salzedas, Luciano T. A. Cintra, João E. G. Filho
Aim
The aim of the present study was to evaluate apical periodontitis (AP) development in rats under a chronic alcohol diet by calcium, phosphorus, and alkaline phosphatase blood levels in addition to histological and radiographic analyses.
Methods
Thirty-two rats were arranged into four groups: (a) group 1: without apical periodontitis and on a regular diet; (b) group 2: AP and on a regular diet; (c) group 3: alcoholic diet without apical periodontitis; and (d) group 4: alcoholic diet and apical periodontitis. Alcoholic solution at 20% was given throughout the 8-week experiment. AP was induced in the first molars at the end of the 7th week. At the end, the animals were anesthetized for blood collection, followed by euthanasia, and jaws were removed for digital radiography and histological processing. The level of significance was 5%.
Results
Calcium levels remained constant in all groups (P >0.05). Group 4 showed a higher phosphorous level than group 2 (P <0.05). The alkaline phosphatase activity was higher in group 3 compared with group 1 (P <0.05). Three animals in group 4 exhibited a severe inflammatory reaction, whereas the animals in group 2 did not demonstrate any reaction (P <0.05). The lowest value of radiographic density was given by group 4 (P <0.05).
Conclusions
Chronic alcohol consumption increased serum phosphorus and decreased bone density in the periapical region, favoring AP development.
{"title":"Chronic alcohol consumption changes blood marker profile and bone density in rats with apical periodontitis","authors":"Renan Dal-Fabbro, Melyna Marques de Almeida, Leopoldo Cosme-Silva, Antonio H. C. Neto, Leda M. P. Salzedas, Luciano T. A. Cintra, João E. G. Filho","doi":"10.1111/jicd.12418","DOIUrl":"10.1111/jicd.12418","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>The aim of the present study was to evaluate apical periodontitis (AP) development in rats under a chronic alcohol diet by calcium, phosphorus, and alkaline phosphatase blood levels in addition to histological and radiographic analyses.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Thirty-two rats were arranged into four groups: (a) group 1: without apical periodontitis and on a regular diet; (b) group 2: AP and on a regular diet; (c) group 3: alcoholic diet without apical periodontitis; and (d) group 4: alcoholic diet and apical periodontitis. Alcoholic solution at 20% was given throughout the 8-week experiment. AP was induced in the first molars at the end of the 7th week. At the end, the animals were anesthetized for blood collection, followed by euthanasia, and jaws were removed for digital radiography and histological processing. The level of significance was 5%.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Calcium levels remained constant in all groups (<i>P </i>><i> </i>0.05). Group 4 showed a higher phosphorous level than group 2 (<i>P </i><<i> </i>0.05). The alkaline phosphatase activity was higher in group 3 compared with group 1 (<i>P </i><<i> </i>0.05). Three animals in group 4 exhibited a severe inflammatory reaction, whereas the animals in group 2 did not demonstrate any reaction (<i>P </i><<i> </i>0.05). The lowest value of radiographic density was given by group 4 (<i>P </i><<i> </i>0.05).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Chronic alcohol consumption increased serum phosphorus and decreased bone density in the periapical region, favoring AP development.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16204,"journal":{"name":"Journal of Investigative and Clinical Dentistry","volume":"10 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/jicd.12418","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37310365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Juan Pacheco-Yanes, Isbelia Gazzaneo, Alejandro R. Pérez, Luciana Armada, Mônica A. S. Neves
Aim
In the present study, we compared the transportation of three different instrumentation systems in different levels of standardized artificial curved canals.
Methods
Sixty epoxy resin blocks containing simulated canals were divided into three groups (n = 20) according to the instrument used for canal preparation: Reciproc (REC), Reciproc Blue (REC Blue), and XP-endo Shaper (XPS). Pre- and post-instrumentation digital images of each specimen were superimposed by Photoshop software to evaluate the different amount of transportation. The Kruskal-Wallis and Wilcoxon were the non-parametric tests applied for the intergroup and intragroup analyses, respectively. The significance level was set at 5%.
Results
The XPS showed significantly less canal transportation than REC Blue at five levels (P <0.05) and significantly less canal transportation at seven evaluated levels when compared with the REC (P <0.05). REC Blue showed significant less canal transportation than REC at four of the eight evaluated levels (P <0.05). All evaluated instruments showed a significantly higher amount of resin removal toward the inner wall compared to the outer wall of the curvature (P <0.001).
Conclusion
Although the XPS showed significantly less canal transportation than REC and REC Blue, all systems produced transportation from the original canal anatomy.
{"title":"Transportation assessment in artificial curved canals after instrumentation with Reciproc, Reciproc Blue, and XP-endo Shaper Systems","authors":"Juan Pacheco-Yanes, Isbelia Gazzaneo, Alejandro R. Pérez, Luciana Armada, Mônica A. S. Neves","doi":"10.1111/jicd.12417","DOIUrl":"10.1111/jicd.12417","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>In the present study, we compared the transportation of three different instrumentation systems in different levels of standardized artificial curved canals.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Sixty epoxy resin blocks containing simulated canals were divided into three groups (<i>n</i> = 20) according to the instrument used for canal preparation: Reciproc (REC), Reciproc Blue (REC Blue), and XP-endo Shaper (XPS). Pre- and post-instrumentation digital images of each specimen were superimposed by Photoshop software to evaluate the different amount of transportation. The Kruskal-Wallis and Wilcoxon were the non-parametric tests applied for the intergroup and intragroup analyses, respectively. The significance level was set at 5%.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The XPS showed significantly less canal transportation than REC Blue at five levels (<i>P </i><<i> </i>0.05) and significantly less canal transportation at seven evaluated levels when compared with the REC (<i>P </i><<i> </i>0.05). REC Blue showed significant less canal transportation than REC at four of the eight evaluated levels (<i>P </i><<i> </i>0.05). All evaluated instruments showed a significantly higher amount of resin removal toward the inner wall compared to the outer wall of the curvature (<i>P </i><<i> </i>0.001).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Although the XPS showed significantly less canal transportation than REC and REC Blue, all systems produced transportation from the original canal anatomy.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16204,"journal":{"name":"Journal of Investigative and Clinical Dentistry","volume":"10 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/jicd.12417","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37128316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mohammad A. Keshvad, Tabassom Hooshmand, Marjan Behroozibakhsh, Sotoodeh Davaei
Aim
The aim of the present study was to investigate the effect of different dentin pretreatments on the interfacial fracture toughness of a self-adhesive flowable composite to dentin compared with that of a conventional flowable composite.
Methods
Caries-free human molars were sectioned to expose the underlying dentin and were randomly divided into seven groups (N = 12) of dentin pretreatments bonded to a self-adhesive flowable composite (Vetise Flow, VF) or a conventional flowable composite (Clearfil Magesty Flow, CM). For VF; Control group (group C-VF), there was no pretreatment, self-etching primer (SP), oxalate dentin desensitizer (OX), and chlorhexidine gluconate (CH) were used. For CM; SP (group SP-CM), OX followed by SP (OX-CM), and CH followed by SP (CH-CM) were used. The interfacial fracture toughness was measured using a universal testing machine. Data were analyzed by Kruskal-Wallis test and analysis of variance.
Results
For VF, the fracture toughness of SP-VF was significantly higher than that of other groups. For CM, a significantly higher fracture toughness for SP-CM than that of OX-CM was found. For all dentin pretreatments, the fracture toughness values were significantly higher for CM compared with the VF.
Conclusions
The self-adhesive flowable composite had reduced bonding efficacy to dentin compared with that of the conventional flowable composite, regardless of the type of dentin pretreatment.
{"title":"Interfacial fracture toughness of self-adhesive and conventional flowable composites to dentin using different dentin pretreatments","authors":"Mohammad A. Keshvad, Tabassom Hooshmand, Marjan Behroozibakhsh, Sotoodeh Davaei","doi":"10.1111/jicd.12414","DOIUrl":"10.1111/jicd.12414","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>The aim of the present study was to investigate the effect of different dentin pretreatments on the interfacial fracture toughness of a self-adhesive flowable composite to dentin compared with that of a conventional flowable composite.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Caries-free human molars were sectioned to expose the underlying dentin and were randomly divided into seven groups (N = 12) of dentin pretreatments bonded to a self-adhesive flowable composite (Vetise Flow, VF) or a conventional flowable composite (Clearfil Magesty Flow, CM). For VF; Control group (group C-VF), there was no pretreatment, self-etching primer (SP), oxalate dentin desensitizer (OX), and chlorhexidine gluconate (CH) were used. For CM; SP (group SP-CM), OX followed by SP (OX-CM), and CH followed by SP (CH-CM) were used. The interfacial fracture toughness was measured using a universal testing machine. Data were analyzed by Kruskal-Wallis test and analysis of variance.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>For VF, the fracture toughness of SP-VF was significantly higher than that of other groups. For CM, a significantly higher fracture toughness for SP-CM than that of OX-CM was found. For all dentin pretreatments, the fracture toughness values were significantly higher for CM compared with the VF.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>The self-adhesive flowable composite had reduced bonding efficacy to dentin compared with that of the conventional flowable composite, regardless of the type of dentin pretreatment.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16204,"journal":{"name":"Journal of Investigative and Clinical Dentistry","volume":"10 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/jicd.12414","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37301140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Natanael V. F. Bezerra, Arella C. M. Brito, Mariana M. D. de Medeiros, Karla L. de França Leite, Isis M. Bezerra, Leopoldina F. D. de Almeida, Carolina P. Aires, Yuri W. Cavalcanti
Aim
Evidence of glucose supplementation effect on Candida biofilm metabolism has not been demonstrated. Therefore, the aim of the present study was to evaluate the effect of glucose concentration on Candida biofilms.
Methods
Single- and dual-species biofilms of Candida were grown on saliva-coated poly(methyl-methacrylate) disks for 72 hours. Biofilms (N = 8/group) were exposed to the following concentrations of glucose: 100 mmol/L (G100), 300 mmol/L (G300), and no glucose (G0: control). Biofilms were collected to determine the acidogenicity, viability, amount of soluble and insoluble extracellular polysaccharides (IEPS), and surface roughness. Data were analyzed using analysis of variance and Tukey's tests (α < 0.05).
Results
Single- and dual-species biofilms from G300 were more acidogenic at 48 and 72 hours compared to G100 and G0 (P <0.05). The viability of the G100 and G300 groups did not differ (P >0.05), but differed statistically from G0. The amount of IEPS in the G300 group was statistically higher than the G0 and G100 groups (P <0.05). The G300 group also presented a higher IEPS proportion per number of viable cells compared to others. G300 presented greater surface roughness for both single- (mean roughness = 1460 μm) and dual-species (mean roughness = 1990 μm) biofilms.
Conclusions
Higher glucose concentration (300 mmol/L) during biofilm development favors the growth of single- and dual-species biofilms of Candida.
{"title":"Glucose supplementation effect on the acidogenicity, viability, and extracellular matrix of Candida single- and dual-species biofilms","authors":"Natanael V. F. Bezerra, Arella C. M. Brito, Mariana M. D. de Medeiros, Karla L. de França Leite, Isis M. Bezerra, Leopoldina F. D. de Almeida, Carolina P. Aires, Yuri W. Cavalcanti","doi":"10.1111/jicd.12412","DOIUrl":"10.1111/jicd.12412","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>Evidence of glucose supplementation effect on <i>Candida</i> biofilm metabolism has not been demonstrated. Therefore, the aim of the present study was to evaluate the effect of glucose concentration on <i>Candida</i> biofilms.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Single- and dual-species biofilms of <i>Candida</i> were grown on saliva-coated poly(methyl-methacrylate) disks for 72 hours. Biofilms (N = 8/group) were exposed to the following concentrations of glucose: 100 mmol/L (G100), 300 mmol/L (G300), and no glucose (G0: control). Biofilms were collected to determine the acidogenicity, viability, amount of soluble and insoluble extracellular polysaccharides (IEPS), and surface roughness. Data were analyzed using analysis of variance and Tukey's tests (α < 0.05).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Single- and dual-species biofilms from G300 were more acidogenic at 48 and 72 hours compared to G100 and G0 (<i>P </i><<i> </i>0.05). The viability of the G100 and G300 groups did not differ (<i>P </i>><i> </i>0.05), but differed statistically from G0. The amount of IEPS in the G300 group was statistically higher than the G0 and G100 groups (<i>P </i><<i> </i>0.05). The G300 group also presented a higher IEPS proportion per number of viable cells compared to others. G300 presented greater surface roughness for both single- (mean roughness = 1460 μm) and dual-species (mean roughness = 1990 μm) biofilms.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Higher glucose concentration (300 mmol/L) during biofilm development favors the growth of single- and dual-species biofilms of <i>Candida</i>.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16204,"journal":{"name":"Journal of Investigative and Clinical Dentistry","volume":"10 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/jicd.12412","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37094545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Gwendolyn Huang, Lynette Moore, Richard M. Logan, Sumant Gue
Aim
The epidemiological features and distribution of pediatric oral and maxillofacial pathology in South Australia, Australia, is unknown. The medical and dental specialties involved in the management of oral and maxillofacial pathology is also unknown. The aim of the present study was to audit oral and maxillofacial pathology specimens submitted for diagnosis in a pediatric tertiary-referral hospital setting.
Methods
Histopathology records were retrieved from the Women's and Children's Hospital, Adelaide over a 16-year period. Age, sex, histopathological diagnosis, location of the lesion, and department involved were recorded. Lesions were classified into 12 categories.
Results
A total of 676 lesions involving the oral and maxillofacial region were collected from patients aged 0-18 years. The mean age was 8.71 years. Diagnosis was not significantly associated with sex (P = 0.123). A total of 97.37% of cases were benign, with connective tissue and salivary gland lesions most frequently biopsied and more frequently biopsied by medical departments. Mucoceles (19.23%) were most commonly diagnosed, followed by dentigerous cysts (5.62%). The Department of Paediatric Dentistry submitted most specimens, followed by the Department of Otolaryngology, the Australian Craniofacial Unit, and the Departments of Paediatric Surgery and Plastics.
Conclusion
The present study provides valuable understanding into the epidemiological features of, and the specialties involved in, oral and maxillofacial histopathology in an Australian pediatric population.
{"title":"Retrospective analysis of South Australian pediatric oral and maxillofacial pathology over a 16-year period","authors":"Gwendolyn Huang, Lynette Moore, Richard M. Logan, Sumant Gue","doi":"10.1111/jicd.12410","DOIUrl":"10.1111/jicd.12410","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>The epidemiological features and distribution of pediatric oral and maxillofacial pathology in South Australia, Australia, is unknown. The medical and dental specialties involved in the management of oral and maxillofacial pathology is also unknown. The aim of the present study was to audit oral and maxillofacial pathology specimens submitted for diagnosis in a pediatric tertiary-referral hospital setting.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Histopathology records were retrieved from the Women's and Children's Hospital, Adelaide over a 16-year period. Age, sex, histopathological diagnosis, location of the lesion, and department involved were recorded. Lesions were classified into 12 categories.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>A total of 676 lesions involving the oral and maxillofacial region were collected from patients aged 0-18 years. The mean age was 8.71 years. Diagnosis was not significantly associated with sex (<i>P </i>= 0.123). A total of 97.37% of cases were benign, with connective tissue and salivary gland lesions most frequently biopsied and more frequently biopsied by medical departments. Mucoceles (19.23%) were most commonly diagnosed, followed by dentigerous cysts (5.62%). The Department of Paediatric Dentistry submitted most specimens, followed by the Department of Otolaryngology, the Australian Craniofacial Unit, and the Departments of Paediatric Surgery and Plastics.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>The present study provides valuable understanding into the epidemiological features of, and the specialties involved in, oral and maxillofacial histopathology in an Australian pediatric population.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16204,"journal":{"name":"Journal of Investigative and Clinical Dentistry","volume":"10 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/jicd.12410","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37094543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The aim of the present systematic review and meta-analysis was to address the following Population, Intervention, Comparison, and Outcome question: Is the efficacy of articaine better than lignocaine in adults requiring dental treatment? Four percent articaine was compared with 2% lignocaine for maxillary and mandibular infiltrations and block anesthesia, and with the principal outcome measures of anesthetic success. Using RevMan software, the weighted anesthesia success rates and 95% confidence intervals (CIs) were estimated and compared using a random-effects model. For combined studies, articaine was more likely to achieve successful anesthesia than lignocaine (N = 18, odds ratio [OR]: 1.92, 95% CI: 1.45-2.56, P <0.00001, I2 = 32%). Maxillary and mandibular infiltration studies showed obvious superiority of articaine to lignocaine (N = 8, OR: 2.50, 95% CI: 1.51-4.15, P =0.0004, I2 = 41%). Maxillary infiltration subgroup analysis showed no significant difference between articaine and lignocaine (N = 5, OR: 1.69, 95% CI: 0.88-3.23, P =0.11, I2 = 19%). For combined mandibular anesthesia studies, articaine was superior to lignocaine (N = 14, OR: 1.99, 95% CI: 1.45-2.72, P <0.0001, I2 = 32%), with further subgroup analysis showing significant differences in both mandibular block anesthesia (N = 11, OR: 1.55, 95% CI: 1.19-2.03, P =0.001), I2 = 0%) and mandibular infiltration (N = 3, OR: 3.87, 95% CI: 2.62-5.72, P <0.00001, I2 = 0%), indicating that articaine is more effective than lignocaine in providing anesthetic success in routine dental procedures.
{"title":"Efficacy of articaine vs lignocaine in maxillary and mandibular infiltration and block anesthesia in the dental treatments of adults: A systematic review and meta-analysis","authors":"Niroshani S. Soysa, Ishani B. Soysa, Neil Alles","doi":"10.1111/jicd.12404","DOIUrl":"10.1111/jicd.12404","url":null,"abstract":"<p>The aim of the present systematic review and meta-analysis was to address the following Population, Intervention, Comparison, and Outcome question: Is the efficacy of articaine better than lignocaine in adults requiring dental treatment? Four percent articaine was compared with 2% lignocaine for maxillary and mandibular infiltrations and block anesthesia, and with the principal outcome measures of anesthetic success. Using RevMan software, the weighted anesthesia success rates and 95% confidence intervals (CIs) were estimated and compared using a random-effects model. For combined studies, articaine was more likely to achieve successful anesthesia than lignocaine (N = 18, odds ratio [OR]: 1.92, 95% CI: 1.45-2.56, <i>P </i><<i> </i>0.00001, <i>I</i><sup>2 </sup>= 32%). Maxillary and mandibular infiltration studies showed obvious superiority of articaine to lignocaine (N = 8, OR: 2.50, 95% CI: 1.51-4.15, <i>P </i>=<i> </i>0.0004, <i>I</i><sup>2 </sup>= 41%). Maxillary infiltration subgroup analysis showed no significant difference between articaine and lignocaine (N = 5, OR: 1.69, 95% CI: 0.88-3.23, <i>P </i>=<i> </i>0.11, <i>I</i><sup>2 </sup>= 19%). For combined mandibular anesthesia studies, articaine was superior to lignocaine (N = 14, OR: 1.99, 95% CI: 1.45-2.72, <i>P </i><<i> </i>0.0001, <i>I</i><sup>2 </sup>= 32%), with further subgroup analysis showing significant differences in both mandibular block anesthesia (N = 11, OR: 1.55, 95% CI: 1.19-2.03, <i>P </i>=<i> </i>0.001), <i>I</i><sup>2 </sup>= 0%) and mandibular infiltration (N = 3, OR: 3.87, 95% CI: 2.62-5.72, <i>P </i><<i> </i>0.00001, <i>I</i><sup>2 </sup>= 0%), indicating that articaine is more effective than lignocaine in providing anesthetic success in routine dental procedures.</p>","PeriodicalId":16204,"journal":{"name":"Journal of Investigative and Clinical Dentistry","volume":"10 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/jicd.12404","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37069784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Chenxuan Wei, Ting Gong, Edmond H. N. Pow, Michael G. Botelho
Aim
The aim of the present study was to investigate the initial stem cell and pre-osteoblast cell adhesion and oxidative response on zirconia in comparison with titanium.
Methods
Human dental pulp stem cells (DPSC) and murine pre-osteoblasts (MC3T3-E1) cells were cultured on zirconia and titanium surfaces, and at 3-, 12-, and 24-hour intervals, cell viability and morphology were determined with tetrazolium based colorimetric assay, scanning electron microscopy, and immunofluorescence analysis. The in situ reactive oxygen species level of both cells on each material surface was examined after 24-hour culture.
Results
Both DPSC and MC3T3-E1 cells revealed comparable morphological features during 24-hour cell adhesion processes, with cells continued expanding of cell size and increasing of cell viability on titanium and zirconia surfaces during 24-hour culture. Zirconia demonstrated relatively higher mean cell viability compared to titanium within 24-hour culture, with significantly higher DPSC viability at 12 hours after seeding (P <0.05). Relatively higher mean reactive oxygen species levels in both DPSC and MC3T3E1 were found on zirconia surfaces after 24-hour culture compared to titanium.
Conclusions
From the results, zirconia as a potential dental implant substrate demonstrated equivalent or better initial cellular responses compared to titanium.
{"title":"Adhesive and oxidative response of stem cell and pre-osteoblasts on titanium and zirconia surfaces in vitro","authors":"Chenxuan Wei, Ting Gong, Edmond H. N. Pow, Michael G. Botelho","doi":"10.1111/jicd.12407","DOIUrl":"10.1111/jicd.12407","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>The aim of the present study was to investigate the initial stem cell and pre-osteoblast cell adhesion and oxidative response on zirconia in comparison with titanium.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Human dental pulp stem cells (DPSC) and murine pre-osteoblasts (MC3T3-E1) cells were cultured on zirconia and titanium surfaces, and at 3-, 12-, and 24-hour intervals, cell viability and morphology were determined with tetrazolium based colorimetric assay, scanning electron microscopy, and immunofluorescence analysis. The in situ reactive oxygen species level of both cells on each material surface was examined after 24-hour culture.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Both DPSC and MC3T3-E1 cells revealed comparable morphological features during 24-hour cell adhesion processes, with cells continued expanding of cell size and increasing of cell viability on titanium and zirconia surfaces during 24-hour culture. Zirconia demonstrated relatively higher mean cell viability compared to titanium within 24-hour culture, with significantly higher DPSC viability at 12 hours after seeding (<i>P </i><<i> </i>0.05). Relatively higher mean reactive oxygen species levels in both DPSC and MC3T3E1 were found on zirconia surfaces after 24-hour culture compared to titanium.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>From the results, zirconia as a potential dental implant substrate demonstrated equivalent or better initial cellular responses compared to titanium.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16204,"journal":{"name":"Journal of Investigative and Clinical Dentistry","volume":"10 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/jicd.12407","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37051634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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