Pub Date : 2001-10-27DOI: 10.1046/J.1439-0450.2001.00476.X
N. Rojas, O. Zamora, J. Cascante, D. Garita, E. Moreno
The comparison of serological responses in a sample of adult, vaccinated and held-infected bovines with Brucella abortus is reported. Indirect enzyme immunoassav (EIA) titration curves and Western blotting tests for smooth-type lipopolysaccharide (S-LPS), rough-type LPS (R-LPS) and lipid A were performed. In the initial screening of sera, an overall prevalence of 20.5% was found, which corresponds to a country with a high incidence of brucellosis. End-point EIA titres against LPS antigens from vaccinated and field-infected cows were not significantly different. However, the absorbance values in the titration curves were significantly higher for S-LPS as compared with the other antigens. A high correlation coefficient (r = 0.933) was obtained when the titres to R-LPS versus lipid A were compared. Western blotting reactions of vaccinated and held-infected animals were indistinguishable. S-LPS, R-LPS and lipid A epitopes were recognized in a heterogeneous manner. In general, the number of bovines that reacted against LPS was higher in the field-infected group, with a stronger binding to S-LPS. Based on our observations, the vaccinated and field-infected bovines are capable of producing similar antibody responses to the Brucella main outer surface antigen, LPS. It should be emphasized that the humoral response of cattle to Brucella LPS contains significant amounts of antibodies to other antigenic moieties of this important surface molecule, which may contribute to the immunity to brucellosis.
{"title":"Comparison of the antibody response in adult cattle against different epitopes of Brucella abortus lipopolysaccharide.","authors":"N. Rojas, O. Zamora, J. Cascante, D. Garita, E. Moreno","doi":"10.1046/J.1439-0450.2001.00476.X","DOIUrl":"https://doi.org/10.1046/J.1439-0450.2001.00476.X","url":null,"abstract":"The comparison of serological responses in a sample of adult, vaccinated and held-infected bovines with Brucella abortus is reported. Indirect enzyme immunoassav (EIA) titration curves and Western blotting tests for smooth-type lipopolysaccharide (S-LPS), rough-type LPS (R-LPS) and lipid A were performed. In the initial screening of sera, an overall prevalence of 20.5% was found, which corresponds to a country with a high incidence of brucellosis. End-point EIA titres against LPS antigens from vaccinated and field-infected cows were not significantly different. However, the absorbance values in the titration curves were significantly higher for S-LPS as compared with the other antigens. A high correlation coefficient (r = 0.933) was obtained when the titres to R-LPS versus lipid A were compared. Western blotting reactions of vaccinated and held-infected animals were indistinguishable. S-LPS, R-LPS and lipid A epitopes were recognized in a heterogeneous manner. In general, the number of bovines that reacted against LPS was higher in the field-infected group, with a stronger binding to S-LPS. Based on our observations, the vaccinated and field-infected bovines are capable of producing similar antibody responses to the Brucella main outer surface antigen, LPS. It should be emphasized that the humoral response of cattle to Brucella LPS contains significant amounts of antibodies to other antigenic moieties of this important surface molecule, which may contribute to the immunity to brucellosis.","PeriodicalId":17659,"journal":{"name":"Journal of veterinary medicine. B, Infectious diseases and veterinary public health","volume":"53 1","pages":"623-9"},"PeriodicalIF":0.0,"publicationDate":"2001-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87609344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2001-10-27DOI: 10.1046/J.1439-0450.2001.00475.X
E. Mateu, M. Martín
Resistance to anti-microbial agents has become one of the main issues in public health strategies world-wide. Much attention has been paid to the emergence of pathogenic micro-organisms such as enterococci or Salmonella that have developed resistance mechanisms that render them almost untreatable with current antibiotics. One of the alleged reasons for such an emergence is the non-medical use of antibiotics, especially in animals. However, only recently have veterinary forums and journals begun to discuss this topic. On the other hand, anti-microbial resistance has also become a problem in veterinary medicine and the number of reports indicating high rates of resistance among animal-originated micro-organisms is considerable. The present review deals with the mechanisms of resistance known for antibiotics in common veterinary use, the problem of anti-microbial resistance in veterinary medicine and the links between the use of antibiotics in animals and the emergence of anti-microbial resistance in humans.
{"title":"Why is anti-microbial resistance a veterinary problem as well?","authors":"E. Mateu, M. Martín","doi":"10.1046/J.1439-0450.2001.00475.X","DOIUrl":"https://doi.org/10.1046/J.1439-0450.2001.00475.X","url":null,"abstract":"Resistance to anti-microbial agents has become one of the main issues in public health strategies world-wide. Much attention has been paid to the emergence of pathogenic micro-organisms such as enterococci or Salmonella that have developed resistance mechanisms that render them almost untreatable with current antibiotics. One of the alleged reasons for such an emergence is the non-medical use of antibiotics, especially in animals. However, only recently have veterinary forums and journals begun to discuss this topic. On the other hand, anti-microbial resistance has also become a problem in veterinary medicine and the number of reports indicating high rates of resistance among animal-originated micro-organisms is considerable. The present review deals with the mechanisms of resistance known for antibiotics in common veterinary use, the problem of anti-microbial resistance in veterinary medicine and the links between the use of antibiotics in animals and the emergence of anti-microbial resistance in humans.","PeriodicalId":17659,"journal":{"name":"Journal of veterinary medicine. B, Infectious diseases and veterinary public health","volume":"13 1","pages":"569-81"},"PeriodicalIF":0.0,"publicationDate":"2001-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81238388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2001-10-27DOI: 10.1046/J.1439-0450.2001.00479.X
H. Bradford, B. Adair, J. C. Foster
Natural killer (NK) cell lysis of target cells by an Fc receptor-mediated mechanism has not been conclusively demonstrated in cattle (Campos and Rossi, Vet. Immunol. Immunopathol. 8, 351-362, 1985), although it is well recognized in other species (Sulica et al., Nat. Immun. 14, 123-133, 1995). To resolve this problem, bovine peripheral blood mononuclear cells were completely depleted of adherent monocyte/macrophage type cells. The resulting enriched population of lymphocytes, was totally devoid of adherent monocytes, but contained up to 2% NK-like cells. On their own, this population had very low background levels of cytotoxicity for virus-infected target cells in 51chromium release assays, but following the addition of virus-specific antibodies, high levels of lysis were observed. This enhanced level of antibody-dependent cytotoxicity demonstrated that bovine NK-like cells can mediate killing of targets by an Fc receptor-mediated mechanism as has been demonstrated for NK cells from other species.
自然杀伤(NK)细胞裂解靶细胞由Fc受体介导的机制尚未在牛中得到最终证实(Campos和Rossi, Vet)。Immunol。免疫病理学杂志。8,351-362,1985)，尽管它在其他物种中被很好地识别(Sulica et al.， Nat. immuni . 14, 123-133, 1995)。为了解决这一问题，我们将牛外周血单核细胞中粘附的单核/巨噬细胞完全去除。由此产生的丰富的淋巴细胞群，完全没有粘附的单核细胞，但含有高达2%的nk样细胞。在51chromium释放试验中，该群体本身对病毒感染的靶细胞具有非常低的细胞毒性背景水平，但在添加病毒特异性抗体后，观察到高水平的裂解。这种抗体依赖性细胞毒性水平的提高表明，牛NK样细胞可以通过Fc受体介导的机制介导靶细胞的杀伤，正如其他物种的NK细胞所证明的那样。
{"title":"Antibody-dependent killing of virus-infected targets by NK-like cells in bovine blood.","authors":"H. Bradford, B. Adair, J. C. Foster","doi":"10.1046/J.1439-0450.2001.00479.X","DOIUrl":"https://doi.org/10.1046/J.1439-0450.2001.00479.X","url":null,"abstract":"Natural killer (NK) cell lysis of target cells by an Fc receptor-mediated mechanism has not been conclusively demonstrated in cattle (Campos and Rossi, Vet. Immunol. Immunopathol. 8, 351-362, 1985), although it is well recognized in other species (Sulica et al., Nat. Immun. 14, 123-133, 1995). To resolve this problem, bovine peripheral blood mononuclear cells were completely depleted of adherent monocyte/macrophage type cells. The resulting enriched population of lymphocytes, was totally devoid of adherent monocytes, but contained up to 2% NK-like cells. On their own, this population had very low background levels of cytotoxicity for virus-infected target cells in 51chromium release assays, but following the addition of virus-specific antibodies, high levels of lysis were observed. This enhanced level of antibody-dependent cytotoxicity demonstrated that bovine NK-like cells can mediate killing of targets by an Fc receptor-mediated mechanism as has been demonstrated for NK cells from other species.","PeriodicalId":17659,"journal":{"name":"Journal of veterinary medicine. B, Infectious diseases and veterinary public health","volume":"3 1","pages":"637-40"},"PeriodicalIF":0.0,"publicationDate":"2001-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82078991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2001-09-15DOI: 10.1046/J.1439-0450.2001.00462.X
M. L. Mendoza-Magaña, M. Ramírez-Herrera, J. M. Dueñas-Jiménez, S. Dueñas-Jiménez
UNLABELLED Pig paramyxovirus of the blue eye disease (PPBED) is a novel member of the paramyxoviridac family which infects pigs. In neonatal pigs it causes neurological damage, whereas in adult pigs it affects the reproductive function. As PPBED damages the new-born pig central nervous system (CNS), it is important to study whether PPBED binds to the membrane proteins of all brain tissue, or selectively binds to neuronal tissue of the brain stem, olfactory bulb, hippocampus, cerebellum, frontal, temporal and parietal brain cortex. It is also important to establish whether it also infects neurones obtained from new-born, 60-day-old and adult pigs, and the role of carbohydrate residues in virus binding. The effect on virus binding of polyclonal antibodies against viral envelope proteins was also studied. Binding studies were performed using dot blot and virus overlay protein binding assays. PPBED was able to bind to membrane proteins from all brain regions, particularly to a protein band of approximately 116 kDa. Neuraminidase treatment of neuronal membrane proteins decreased virus binding; subsequent treatments with beta-galactosidase and manosidase did not increase virus binding inhibition. N-glycosidase F and trypsin also decreased virus binding, but not the O-glycanase. Antibodies against viral haemagglutinin-neuraminidase blocked virus binding more efficiently than antibodies against viral fusion protein. IN CONCLUSION (1) PPBFD is able to bind to pig neurones of all brain regions studied and at all ages analysed; (2) a 116 kDa membrane protein containing sialic acid residues with an N-linked oligosaccharide chain was specifically recognized; (3) PPBED haemagglutinin-neuraminidase protein seems to play a central role in neural receptor recognition.
{"title":"Pig paramyxovirus of the blue eye disease binding to a 116 kDa glycoprotein expressed in pig neuronal membranes.","authors":"M. L. Mendoza-Magaña, M. Ramírez-Herrera, J. M. Dueñas-Jiménez, S. Dueñas-Jiménez","doi":"10.1046/J.1439-0450.2001.00462.X","DOIUrl":"https://doi.org/10.1046/J.1439-0450.2001.00462.X","url":null,"abstract":"UNLABELLED\u0000Pig paramyxovirus of the blue eye disease (PPBED) is a novel member of the paramyxoviridac family which infects pigs. In neonatal pigs it causes neurological damage, whereas in adult pigs it affects the reproductive function. As PPBED damages the new-born pig central nervous system (CNS), it is important to study whether PPBED binds to the membrane proteins of all brain tissue, or selectively binds to neuronal tissue of the brain stem, olfactory bulb, hippocampus, cerebellum, frontal, temporal and parietal brain cortex. It is also important to establish whether it also infects neurones obtained from new-born, 60-day-old and adult pigs, and the role of carbohydrate residues in virus binding. The effect on virus binding of polyclonal antibodies against viral envelope proteins was also studied. Binding studies were performed using dot blot and virus overlay protein binding assays. PPBED was able to bind to membrane proteins from all brain regions, particularly to a protein band of approximately 116 kDa. Neuraminidase treatment of neuronal membrane proteins decreased virus binding; subsequent treatments with beta-galactosidase and manosidase did not increase virus binding inhibition. N-glycosidase F and trypsin also decreased virus binding, but not the O-glycanase. Antibodies against viral haemagglutinin-neuraminidase blocked virus binding more efficiently than antibodies against viral fusion protein.\u0000\u0000\u0000IN CONCLUSION\u0000(1) PPBFD is able to bind to pig neurones of all brain regions studied and at all ages analysed; (2) a 116 kDa membrane protein containing sialic acid residues with an N-linked oligosaccharide chain was specifically recognized; (3) PPBED haemagglutinin-neuraminidase protein seems to play a central role in neural receptor recognition.","PeriodicalId":17659,"journal":{"name":"Journal of veterinary medicine. B, Infectious diseases and veterinary public health","volume":"44 1","pages":"489-99"},"PeriodicalIF":0.0,"publicationDate":"2001-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73816616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2001-09-15DOI: 10.1046/J.1439-0450.2001.00469.X
Ulf Magnusson, A. Mörner, A. Persson, E. Karlstam, S. Sternberg, H. Kindahl
The aim of this study was to identify factors that influence the development of disease in sows inoculated with Escherichia coli in the mammary gland. Ten cross-bred primiparous sows were intramammarily inoculated with living E. coli bacteria at different time points before parturition: seven sows within 48 h before parturition and three sows approximately 96 h before parturition. Before and after inoculation, blood samples and mammary gland biopsy specimens were collected and clinical observations were made. All seven sows inoculated close to parturition developed a rectal temperature of >39.5 degrees C during the first 48 h post-partum and two of them also showed other signs of clinical disease. In the sows inoculated 4 days before parturition, the rectal temperature never exceeded 39.5 degrees C during the first 48 h post-partum and none of them showed any other sign of clinical discase. There was a tendency (P < 0.1) that histological signs of mastitis were more frequent in the sows inoculated close to parturition. There were no overall differences between the two groups of sows in plasma concentrations of cortisol, oestradiol-17beta and 15-ketodihydro-PGF2alpha before inoculation. Before inoculation, the number of neutrophils in the blood was overall higher (P < 0.05) in the group of sows that were inoculated close to parturition. In comparison, the number of lymphocytes before inoculation had a tendency (P < 0.1) to be lower in that group. The data suggest that the time of infection of the mammary gland relative to parturition and the number of circulating neutrophils at the time of infection influence the development of chinical coliform mastitis in the sow.
{"title":"Sows intramammarily inoculated with Escherichia coli influence of time of infection, hormone concentrations and leucocyte numbers on development of disease.","authors":"Ulf Magnusson, A. Mörner, A. Persson, E. Karlstam, S. Sternberg, H. Kindahl","doi":"10.1046/J.1439-0450.2001.00469.X","DOIUrl":"https://doi.org/10.1046/J.1439-0450.2001.00469.X","url":null,"abstract":"The aim of this study was to identify factors that influence the development of disease in sows inoculated with Escherichia coli in the mammary gland. Ten cross-bred primiparous sows were intramammarily inoculated with living E. coli bacteria at different time points before parturition: seven sows within 48 h before parturition and three sows approximately 96 h before parturition. Before and after inoculation, blood samples and mammary gland biopsy specimens were collected and clinical observations were made. All seven sows inoculated close to parturition developed a rectal temperature of >39.5 degrees C during the first 48 h post-partum and two of them also showed other signs of clinical disease. In the sows inoculated 4 days before parturition, the rectal temperature never exceeded 39.5 degrees C during the first 48 h post-partum and none of them showed any other sign of clinical discase. There was a tendency (P < 0.1) that histological signs of mastitis were more frequent in the sows inoculated close to parturition. There were no overall differences between the two groups of sows in plasma concentrations of cortisol, oestradiol-17beta and 15-ketodihydro-PGF2alpha before inoculation. Before inoculation, the number of neutrophils in the blood was overall higher (P < 0.05) in the group of sows that were inoculated close to parturition. In comparison, the number of lymphocytes before inoculation had a tendency (P < 0.1) to be lower in that group. The data suggest that the time of infection of the mammary gland relative to parturition and the number of circulating neutrophils at the time of infection influence the development of chinical coliform mastitis in the sow.","PeriodicalId":17659,"journal":{"name":"Journal of veterinary medicine. B, Infectious diseases and veterinary public health","volume":"20 1","pages":"501-12"},"PeriodicalIF":0.0,"publicationDate":"2001-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75442387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2001-09-15DOI: 10.1046/J.1439-0450.2001.00471.X
M. Ryll, Henrik Christensen, Magne Bisgaard, Jens Peter Christensen, K.‐H. Hinz, B. Köhler
A total of 44 bacterial strains obtained from 49 clinically healthy ducklings of different ages originating from four different farms were identified as members of the species Riemerella anatipestifer (RA) using conventional biochemical test methods. Numerical analysis of the whole-cell fatty acid patterns of these isolates resulted in two different clusters, one of which showed a similar pattern to that of the type strain of RA. Strains having a different fatty acid methyl esters (FAME)-profile (cluster II) were designated R. anatipestifer-like (RA-like). Sequencing of 16S rRNA genes of RA-like field isolates revealed 99% identity to RA. The significance of these observations are discussed. The present findings document for the first time that RA seems to represent a normal part of the pharyngeal flora of healthy Pekin ducks.
{"title":"Studies on the prevalence of Riemerella anatipestifer in the upper respiratory tract of clinically healthy ducklings and characterization of untypable strains.","authors":"M. Ryll, Henrik Christensen, Magne Bisgaard, Jens Peter Christensen, K.‐H. Hinz, B. Köhler","doi":"10.1046/J.1439-0450.2001.00471.X","DOIUrl":"https://doi.org/10.1046/J.1439-0450.2001.00471.X","url":null,"abstract":"A total of 44 bacterial strains obtained from 49 clinically healthy ducklings of different ages originating from four different farms were identified as members of the species Riemerella anatipestifer (RA) using conventional biochemical test methods. Numerical analysis of the whole-cell fatty acid patterns of these isolates resulted in two different clusters, one of which showed a similar pattern to that of the type strain of RA. Strains having a different fatty acid methyl esters (FAME)-profile (cluster II) were designated R. anatipestifer-like (RA-like). Sequencing of 16S rRNA genes of RA-like field isolates revealed 99% identity to RA. The significance of these observations are discussed. The present findings document for the first time that RA seems to represent a normal part of the pharyngeal flora of healthy Pekin ducks.","PeriodicalId":17659,"journal":{"name":"Journal of veterinary medicine. B, Infectious diseases and veterinary public health","volume":"34 3 1","pages":"537-46"},"PeriodicalIF":0.0,"publicationDate":"2001-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85768237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2001-09-15DOI: 10.1046/J.1439-0450.2001.00456.X
Librado Carrasco, J. S. Lima, D. Halfen, F. Salguero, P. Sánchez-Cordón, G. Becker
This report describes a case of fatal aspergillosis caused by A. fumigatus during the recovery of an oiled Magallanic penguin. The possible role of aspergillosis as a possible complication responsible for the mortality of penguins surviving the first days of treatment for oil is emphasized.
{"title":"Systemic aspergillosis in an oiled magallanic penguin (Spheniscus magellanicus).","authors":"Librado Carrasco, J. S. Lima, D. Halfen, F. Salguero, P. Sánchez-Cordón, G. Becker","doi":"10.1046/J.1439-0450.2001.00456.X","DOIUrl":"https://doi.org/10.1046/J.1439-0450.2001.00456.X","url":null,"abstract":"This report describes a case of fatal aspergillosis caused by A. fumigatus during the recovery of an oiled Magallanic penguin. The possible role of aspergillosis as a possible complication responsible for the mortality of penguins surviving the first days of treatment for oil is emphasized.","PeriodicalId":17659,"journal":{"name":"Journal of veterinary medicine. B, Infectious diseases and veterinary public health","volume":"413 1","pages":"551-4"},"PeriodicalIF":0.0,"publicationDate":"2001-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76457039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2001-09-15DOI: 10.1046/J.1439-0450.2001.00457.X
S. Sokkar, M. Hamouda, S. M. El-Rahman
The endometrial necropsies of 165 she donkeys were examined and the endometria were classified into four grades according to histological features (I, IIa, IIb, III). Category I was observed in 86 cases (52.12%) and diagnosed as normal endometria. Category IIa was observed in 18 cases (10.91%) and diagnosed as slight endometritis. Category IIb was observed in 28 cases (16.97%) and diagnosed as moderate endometritis (two to four nests/field), while category III was observed in 33 cases (20%) and diagnosed as severe endometritis (eight nests/field). A bacteriological examination was carried out and oestrogen and progesterone were estimated. Our results indicate that endometritis and the prognosis of fertility in she donkeys were more or less parallel to mares.
{"title":"Endometritis in she donkeys in Egypt.","authors":"S. Sokkar, M. Hamouda, S. M. El-Rahman","doi":"10.1046/J.1439-0450.2001.00457.X","DOIUrl":"https://doi.org/10.1046/J.1439-0450.2001.00457.X","url":null,"abstract":"The endometrial necropsies of 165 she donkeys were examined and the endometria were classified into four grades according to histological features (I, IIa, IIb, III). Category I was observed in 86 cases (52.12%) and diagnosed as normal endometria. Category IIa was observed in 18 cases (10.91%) and diagnosed as slight endometritis. Category IIb was observed in 28 cases (16.97%) and diagnosed as moderate endometritis (two to four nests/field), while category III was observed in 33 cases (20%) and diagnosed as severe endometritis (eight nests/field). A bacteriological examination was carried out and oestrogen and progesterone were estimated. Our results indicate that endometritis and the prognosis of fertility in she donkeys were more or less parallel to mares.","PeriodicalId":17659,"journal":{"name":"Journal of veterinary medicine. B, Infectious diseases and veterinary public health","volume":"4 1","pages":"529-36"},"PeriodicalIF":0.0,"publicationDate":"2001-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80213617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2001-09-15DOI: 10.1046/J.1439-0450.2001.00468.X
H. Yoshimura, M. Ishimaru, Y. S. Endoh, A. Kojima
Minimum inhibitory concentrations (MICs) of 10 antimicrobial agents were determined for Pasteurella multocida from cattle and pigs (72 and 68 isolates, respectively). Higher MICs were observed with oxytetracycline, doxycycline, tilmicosin and thiamphenicol for porcine isolates than for bovine isolates. Enrofloxacin was the most active, with an MIC for 90% of the isolates (MIC90) of 0.05 microg/ml for both bovine and porcine isolates. Aspoxicillin exhibited the same excellent activity against penicillin-susceptible isolates as ceftiofur, with MICs ranging from < or = 0.025 to 0.1 microg/ml. Aminoglycosides were less active, with an MIC90 of > 100 microg/ml for both bovine and porcine isolates.
{"title":"Antimicrobial susceptibility of Pasteurella multocida isolated from cattle and pigs.","authors":"H. Yoshimura, M. Ishimaru, Y. S. Endoh, A. Kojima","doi":"10.1046/J.1439-0450.2001.00468.X","DOIUrl":"https://doi.org/10.1046/J.1439-0450.2001.00468.X","url":null,"abstract":"Minimum inhibitory concentrations (MICs) of 10 antimicrobial agents were determined for Pasteurella multocida from cattle and pigs (72 and 68 isolates, respectively). Higher MICs were observed with oxytetracycline, doxycycline, tilmicosin and thiamphenicol for porcine isolates than for bovine isolates. Enrofloxacin was the most active, with an MIC for 90% of the isolates (MIC90) of 0.05 microg/ml for both bovine and porcine isolates. Aspoxicillin exhibited the same excellent activity against penicillin-susceptible isolates as ceftiofur, with MICs ranging from < or = 0.025 to 0.1 microg/ml. Aminoglycosides were less active, with an MIC90 of > 100 microg/ml for both bovine and porcine isolates.","PeriodicalId":17659,"journal":{"name":"Journal of veterinary medicine. B, Infectious diseases and veterinary public health","volume":"34 1","pages":"555-60"},"PeriodicalIF":0.0,"publicationDate":"2001-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81189341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2001-09-15DOI: 10.1046/J.1439-0450.2001.00470.X
Songhua Hu, C. Concha, A. Johannisson, G. Meglia, K. P. Waller
Cows with subclinical mastitis caused by Staphylococcus aureus were subjected to subcutaneous injections with either an extract from the root of Panax ginseng CA Meyer at a dose of 8 mg/kg body weight per day for 6 days, or with saline as a control. The injection areas were checked for adverse reactions. The daily milk production was measured before and after treatment. Blood was collected for total and differential leucocyte counts, identification of lymphocyte subpopulations using flow cytometry, lymphocyte proliferation test, and neutrophil phagocytosis and oxidative burst assay. Quarter milk samples were collected for bacteriological analysis and somatic cell counts (SCC). After the end of treatment, the numbers of S. aureus-infected quarters and milk SCC tended to decrease in ginseng-treated cows. Phagocytosis and oxidative burst activity of blood neutrophils were significantly increased 1 week after ginseng treatment, but the proliferative response of blood lymphocytes did not change significantly. The number of monocytes in ginseng-injected cows was significantly higher 1 week post-treatment than pre-treatment, and the number of lymphocytes was significantly higher than pre-infusion at 2 and 3 weeks after ginseng treatment. Similar changes were not observed in the control group. The present findings indicate that ginseng treatment can activate the innate immunity of cows and may contribute to the cow's recovery from mastitis. It is therefore suggested that ginseng has a potential as a stimulator of the immune system of dairy cows.
{"title":"Effect of subcutaneous injection of ginseng on cows with subclinical Staphylococcus aureus mastitis.","authors":"Songhua Hu, C. Concha, A. Johannisson, G. Meglia, K. P. Waller","doi":"10.1046/J.1439-0450.2001.00470.X","DOIUrl":"https://doi.org/10.1046/J.1439-0450.2001.00470.X","url":null,"abstract":"Cows with subclinical mastitis caused by Staphylococcus aureus were subjected to subcutaneous injections with either an extract from the root of Panax ginseng CA Meyer at a dose of 8 mg/kg body weight per day for 6 days, or with saline as a control. The injection areas were checked for adverse reactions. The daily milk production was measured before and after treatment. Blood was collected for total and differential leucocyte counts, identification of lymphocyte subpopulations using flow cytometry, lymphocyte proliferation test, and neutrophil phagocytosis and oxidative burst assay. Quarter milk samples were collected for bacteriological analysis and somatic cell counts (SCC). After the end of treatment, the numbers of S. aureus-infected quarters and milk SCC tended to decrease in ginseng-treated cows. Phagocytosis and oxidative burst activity of blood neutrophils were significantly increased 1 week after ginseng treatment, but the proliferative response of blood lymphocytes did not change significantly. The number of monocytes in ginseng-injected cows was significantly higher 1 week post-treatment than pre-treatment, and the number of lymphocytes was significantly higher than pre-infusion at 2 and 3 weeks after ginseng treatment. Similar changes were not observed in the control group. The present findings indicate that ginseng treatment can activate the innate immunity of cows and may contribute to the cow's recovery from mastitis. It is therefore suggested that ginseng has a potential as a stimulator of the immune system of dairy cows.","PeriodicalId":17659,"journal":{"name":"Journal of veterinary medicine. B, Infectious diseases and veterinary public health","volume":"95 1","pages":"519-28"},"PeriodicalIF":0.0,"publicationDate":"2001-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84949868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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