Ona cina ( Lantana camara) is a plant that has the ability as an anti-bacterial. The bioactive compounds of the ona cina plant as anti-bacterial are flavonols and lantanilic acid. This study aimed to compared the methanol extract of the leaves and roots of the ona cina plant in inhibiting the growth of Staphylococcus aureus and Pseudomonas aeruginosa . This research is a laboratory experimental study using the well diffusion method with 24 treatments. The treatment was repeated 2 times with various concentrations of 0.5%, 5%, 10%, 25%, and 50%, and erythromycin as a positive control and aquades as a negative control. The methanol extract of the leaves and roots of the ona cina plant was obtained from the maceration process with methanol solvent, then the growth inhibition test was carried out on the test bacteria. The parameters observed were the clear zone formed around the well.The diameter of the inhibition zone for the growth of the test bacteria at all concentrations of the leaf methanol extract of the ona cina plant was 0 mm while for the methanol extract of the ona cina plant root, the diameter of the inhibition zone on the growth of Staphylococcus aureus at a concentration of 2.5% was 17 mm and the highest is found at a concentration of 50% of 25 mm. While the lowest inhibition zone of root methanol extract on the growth of Pseudomonas aeruginosa was at a concentration of 2.5% at 22 mm and the highest was at a concentration of 50% at 30 mm. The methanol extract of the leaves of the ona cina plant did not have the ability to inhibit the growth of the test bacteria. However, the methanol extract of the ona cina root has the ability to inhibit the growth of Staphylococcus aureus and Pseudomonas aeruginosa .
{"title":"Perbandingan Daya Hambat Ekstrak Metanol Daun dan Akar Tanaman Ona cina (Lantana camara) Terhadap Pertumbuhan Staphylococcus aureus dan Pseudomonas aeruginosa","authors":"Fikram Umaternate, E. Astuty, P. Y. Silalahi","doi":"10.26630/jak.v12i1.3695","DOIUrl":"https://doi.org/10.26630/jak.v12i1.3695","url":null,"abstract":"Ona cina ( Lantana camara) is a plant that has the ability as an anti-bacterial. The bioactive compounds of the ona cina plant as anti-bacterial are flavonols and lantanilic acid. This study aimed to compared the methanol extract of the leaves and roots of the ona cina plant in inhibiting the growth of Staphylococcus aureus and Pseudomonas aeruginosa . This research is a laboratory experimental study using the well diffusion method with 24 treatments. The treatment was repeated 2 times with various concentrations of 0.5%, 5%, 10%, 25%, and 50%, and erythromycin as a positive control and aquades as a negative control. The methanol extract of the leaves and roots of the ona cina plant was obtained from the maceration process with methanol solvent, then the growth inhibition test was carried out on the test bacteria. The parameters observed were the clear zone formed around the well.The diameter of the inhibition zone for the growth of the test bacteria at all concentrations of the leaf methanol extract of the ona cina plant was 0 mm while for the methanol extract of the ona cina plant root, the diameter of the inhibition zone on the growth of Staphylococcus aureus at a concentration of 2.5% was 17 mm and the highest is found at a concentration of 50% of 25 mm. While the lowest inhibition zone of root methanol extract on the growth of Pseudomonas aeruginosa was at a concentration of 2.5% at 22 mm and the highest was at a concentration of 50% at 30 mm. The methanol extract of the leaves of the ona cina plant did not have the ability to inhibit the growth of the test bacteria. However, the methanol extract of the ona cina root has the ability to inhibit the growth of Staphylococcus aureus and Pseudomonas aeruginosa .","PeriodicalId":17791,"journal":{"name":"Jurnal Media Analis Kesehatan","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82077603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Alma Tiara Rahayu, Aldi Pratama, M. Wahyu Setiawan, Ma'rifatussolihat Ma'rifatussolihat, Nurul Azmah Nikmatullah
In Indonesia, helminth infection is one of the diseases with a fairly large number. Soil Transmitted Helminth (STH) is the transmission of helminth infection through the soil. Examination of feces is carried out to diagnose helminth infections by finding infective eggs or larvae. Deworming analysis can be done quantitatively or qualitatively. The flotation, sedimentation and native (direct slide) method is a qualitative worm infection examination method to detect intestinal parasites, while the Kato-Katz method is a quantitative method of detecting helminth infections. Purpose: to find out the optimal concentration and duration of flotation in the flotation method using solutions of ZnSO , MgSO and NaCl. The method used is the centrifugal flotation method. The samples were screened and 12 positive samples were obtained. Results: found egg type of worm Ascaris lumbricoides with fertile, infertile and decorticated forms. At a concentration of 33% 10 minutes, a solution of ZnSO 53 eggs was obtained, MgSO 16 eggs, NaCl 10 eggs while 20 minutes ZnSO 29 eggs, MgSO 39 eggs, NaCl 19 eggs were obtained. At a concentration of 43% time 10 minutes, ZnSO 29 eggs were obtained, MgSO 17 eggs, NaCl 11 eggs while a time of 20 minutes was obtained ZnSO 34 eggs, MgSO 26 eggs, NaCl 1 egg. Conclusion: the optimal concentration and flotation time in ZnSO solution is a concentration of 33% with a time of 10 minutes, mgSO solution with a concentration of 33% for 20 minutes, and a NaCl solution with a concentration of 33% for 20 minutes.
{"title":"OPTIMASI METODE FLOTASI SENTRIFUS MENGGUNAKAN LARUTAN ZnSO₄, MgSO₄, DAN NaCl BERDASARKAN KONSENTRASI LARUTAN DAN LAMA PENGAPUNGAN","authors":"Alma Tiara Rahayu, Aldi Pratama, M. Wahyu Setiawan, Ma'rifatussolihat Ma'rifatussolihat, Nurul Azmah Nikmatullah","doi":"10.32382/mak.v14i1.3283","DOIUrl":"https://doi.org/10.32382/mak.v14i1.3283","url":null,"abstract":"In Indonesia, helminth infection is one of the diseases with a fairly large number. Soil Transmitted Helminth (STH) is the transmission of helminth infection through the soil. Examination of feces is carried out to diagnose helminth infections by finding infective eggs or larvae. Deworming analysis can be done quantitatively or qualitatively. The flotation, sedimentation and native (direct slide) method is a qualitative worm infection examination method to detect intestinal parasites, while the Kato-Katz method is a quantitative method of detecting helminth infections. Purpose: to find out the optimal concentration and duration of flotation in the flotation method using solutions of ZnSO , MgSO and NaCl. The method used is the centrifugal flotation method. The samples were screened and 12 positive samples were obtained. Results: found egg type of worm Ascaris lumbricoides with fertile, infertile and decorticated forms. At a concentration of 33% 10 minutes, a solution of ZnSO 53 eggs was obtained, MgSO 16 eggs, NaCl 10 eggs while 20 minutes ZnSO 29 eggs, MgSO 39 eggs, NaCl 19 eggs were obtained. At a concentration of 43% time 10 minutes, ZnSO 29 eggs were obtained, MgSO 17 eggs, NaCl 11 eggs while a time of 20 minutes was obtained ZnSO 34 eggs, MgSO 26 eggs, NaCl 1 egg. Conclusion: the optimal concentration and flotation time in ZnSO solution is a concentration of 33% with a time of 10 minutes, mgSO solution with a concentration of 33% for 20 minutes, and a NaCl solution with a concentration of 33% for 20 minutes.","PeriodicalId":17791,"journal":{"name":"Jurnal Media Analis Kesehatan","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135751379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yaumil Fachni Tandjungbulu, Herman Herman, Nurdin Nurdin, Alfin Resya Virgiawan, M. Askar, Besse Nurfadillah
Infeksi Saluran Kemih (ISK) merupakan invasi mikroorganisme yang menyerang pada salah satu atau beberapa bagian organ saluran kemih yang dapat disebabkan oleh bakteri, jamur, dan virus. ISK dapat menyebabkan manifestasi klinis yang sangat bervariasi, dapat menyerang organ perkemihan seperti uretra ( urethritis ), kandung kemih ( cystisis ), ureter ( ureteritis ), bahkan dapat menyerang organ ginjal yang dapat menyebabkan kerusakan pada fungsi ginjal. Tujuan penelitian ini untuk mengetahui variasi hasil pemeriksaan sedimen urin pada pasien suspek ISK. Jenis penelitian bersifat observasional laboratorik dengan rancangan cross section. Penelitian ini dilaksanakan di Rumah Sakit Perguruan Tinggi Negeri Universitas Hasanuddin (RSPTN-UH) Makassar pada tanggal 31 Mei-13 Juni 2022. Sampel dalam penelitian berjumlah 67 orang pasien suspek ISK, dengan teknik pengambilan sampel purposive sampling. Hasil penelitian menunjukkan bahwa berdasarkan karakteristik subjek penelitian jenis kelamin laki-laki sebanyak 31 orang (46,3%) dan perempuan sebanyak 36 orang (53,7%), untuk klasifikasi umur terbanyak yaitu umur 45-54 tahun sebanyak 18 orang (26,9%) dan yang paling sedikit yaitu pasien dengan umur 25-34 tahun sebanyak 3 orang (4,5%). Untuk variasi hasil pemeriksaan sedimen urin pasien dengan keadaan eritrosit normal sebanyak 55 orang (82,1%) dan abnormal sebanyak 12 orang (17,9%), sedangkan pasien dengan keadaan leukosit normal sebanyak 56 orang (83,6%) dan abnormal sebanyak 11 orang (16,4%). Pemeriksaan sel epitel normal sebanyak 63 orang (94,0%) dan abnormal sebanyak 4 orang (6,0%), pemeriksaan kristal normal sebanyak 63 orang (94,0%) dan abnormal sebanyak 4 orang (6,0%), dari 67 sampel penelitian tidak ditemukan adanya silinder dan bakteri pada urin pasien suspek ISK (0%). Berdasarkan hasil dalam penelitian ini dapat disimpulkan bahwa terdapat lebih banyak sel normal dibandingkan abnormal pada urin pasien suspek ISK. Hal ini dapat disebabkan karena peneliti tidak mengontrol beberapa hal yang dapat mempengaruhi hasil pemeriksaan sedimen urin salah satunya penggunaan terapi pengobatan antibiotik pada subjek penelitian, sehingga subjek yang telah mengonsumsi antibiotik tersebut tidak ditemukan adanya bakteri di dalam urinnya karena zat atau kandungan yang terdapat pada antibiotik dapat menghambat perkembangbiakan dan menghentikan penyebaran serta pertumbuhan bakteri pada urin pasien suspek dan penderita ISK. Sehingga disarankan dalam penegakan diagnosa ISK dapat menggunakan marker pemeriksaan tambahan yang lebih spesifik seperti kultur urin dan pemeriksaan urin rutin untuk mendeteksi adanya infeksi pada saluran perkemihan, dan memperhatikan persiapan pasien sebelum pemeriksaan dilakukan.
{"title":"VARIASI HASIL PEMERIKSAAN SEDIMEN URIN PADA PASIEN SUSPEK INFEKSI SALURAN KEMIH","authors":"Yaumil Fachni Tandjungbulu, Herman Herman, Nurdin Nurdin, Alfin Resya Virgiawan, M. Askar, Besse Nurfadillah","doi":"10.32382/mak.v14i1.3263","DOIUrl":"https://doi.org/10.32382/mak.v14i1.3263","url":null,"abstract":"Infeksi Saluran Kemih (ISK) merupakan invasi mikroorganisme yang menyerang pada salah satu atau beberapa bagian organ saluran kemih yang dapat disebabkan oleh bakteri, jamur, dan virus. ISK dapat menyebabkan manifestasi klinis yang sangat bervariasi, dapat menyerang organ perkemihan seperti uretra ( urethritis ), kandung kemih ( cystisis ), ureter ( ureteritis ), bahkan dapat menyerang organ ginjal yang dapat menyebabkan kerusakan pada fungsi ginjal. Tujuan penelitian ini untuk mengetahui variasi hasil pemeriksaan sedimen urin pada pasien suspek ISK. Jenis penelitian bersifat observasional laboratorik dengan rancangan cross section. Penelitian ini dilaksanakan di Rumah Sakit Perguruan Tinggi Negeri Universitas Hasanuddin (RSPTN-UH) Makassar pada tanggal 31 Mei-13 Juni 2022. Sampel dalam penelitian berjumlah 67 orang pasien suspek ISK, dengan teknik pengambilan sampel purposive sampling. Hasil penelitian menunjukkan bahwa berdasarkan karakteristik subjek penelitian jenis kelamin laki-laki sebanyak 31 orang (46,3%) dan perempuan sebanyak 36 orang (53,7%), untuk klasifikasi umur terbanyak yaitu umur 45-54 tahun sebanyak 18 orang (26,9%) dan yang paling sedikit yaitu pasien dengan umur 25-34 tahun sebanyak 3 orang (4,5%). Untuk variasi hasil pemeriksaan sedimen urin pasien dengan keadaan eritrosit normal sebanyak 55 orang (82,1%) dan abnormal sebanyak 12 orang (17,9%), sedangkan pasien dengan keadaan leukosit normal sebanyak 56 orang (83,6%) dan abnormal sebanyak 11 orang (16,4%). Pemeriksaan sel epitel normal sebanyak 63 orang (94,0%) dan abnormal sebanyak 4 orang (6,0%), pemeriksaan kristal normal sebanyak 63 orang (94,0%) dan abnormal sebanyak 4 orang (6,0%), dari 67 sampel penelitian tidak ditemukan adanya silinder dan bakteri pada urin pasien suspek ISK (0%). Berdasarkan hasil dalam penelitian ini dapat disimpulkan bahwa terdapat lebih banyak sel normal dibandingkan abnormal pada urin pasien suspek ISK. Hal ini dapat disebabkan karena peneliti tidak mengontrol beberapa hal yang dapat mempengaruhi hasil pemeriksaan sedimen urin salah satunya penggunaan terapi pengobatan antibiotik pada subjek penelitian, sehingga subjek yang telah mengonsumsi antibiotik tersebut tidak ditemukan adanya bakteri di dalam urinnya karena zat atau kandungan yang terdapat pada antibiotik dapat menghambat perkembangbiakan dan menghentikan penyebaran serta pertumbuhan bakteri pada urin pasien suspek dan penderita ISK. Sehingga disarankan dalam penegakan diagnosa ISK dapat menggunakan marker pemeriksaan tambahan yang lebih spesifik seperti kultur urin dan pemeriksaan urin rutin untuk mendeteksi adanya infeksi pada saluran perkemihan, dan memperhatikan persiapan pasien sebelum pemeriksaan dilakukan.","PeriodicalId":17791,"journal":{"name":"Jurnal Media Analis Kesehatan","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135751532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Malaria is one of the causes of death and morbidity in children and adults in tropical countries. Malaria is caused by Protozoa of the genus Plasmodium , Plasmodium in humans consists of 4 species, namely Palsmodium falciparum, Palsmodium vivax, Palsmodium malariae and Palsmodium ovale . Pesawaran District is the area most commonly found with malaria, in 2017 there were 1,738 cases of malaria in the working area of the Hanura Health Center. The purpose of this study was to find out and identify Plasmodium malaria species that infect people in the work area of the Hanura Village Public Health Center, Teluk Pandan District, Pesawaran Regency, Lampung. This type of research is a descriptive survey research using cross-sectional methods using univariate data analysis. The data in this study were primary data, namely the results of Plasmodium identification in blood smears and in the form of patient demographic data (age, sex, education and behavior using mosquito nets or not). The results showed that out of 164 respondents, 22 patients found Plasmodium parasites. The identified Plasmodium vivax parasites were 20 people (90.9%) while for Plasmodium falciparum there were 2 people (9.1%). Plasmodium parasites identified at the age of 5-11 years (children) were 6 respondents (27.3%), in female sex there were 13 people (59.1%), at the elementary school level there were 15 people (68.2%), the behavior of not using mosquito nets was 12 people (54.5%). From the results of this study it can be concluded that Plasmodium parasites were found and identified that infect people in the work area at the Hanura Village Health Center, Teluk Pandan District, Pesawaran Regency.
{"title":"Identifikasi Spesies Plasmodium Malaria Di Wilayah Kerja Puskesmas Desa Hanura Pesawaran Lampung","authors":"Eko Prasetyo, Dwi Haryatmi","doi":"10.26630/jak.v12i1.3243","DOIUrl":"https://doi.org/10.26630/jak.v12i1.3243","url":null,"abstract":"Malaria is one of the causes of death and morbidity in children and adults in tropical countries. Malaria is caused by Protozoa of the genus Plasmodium , Plasmodium in humans consists of 4 species, namely Palsmodium falciparum, Palsmodium vivax, Palsmodium malariae and Palsmodium ovale . Pesawaran District is the area most commonly found with malaria, in 2017 there were 1,738 cases of malaria in the working area of the Hanura Health Center. The purpose of this study was to find out and identify Plasmodium malaria species that infect people in the work area of the Hanura Village Public Health Center, Teluk Pandan District, Pesawaran Regency, Lampung. This type of research is a descriptive survey research using cross-sectional methods using univariate data analysis. The data in this study were primary data, namely the results of Plasmodium identification in blood smears and in the form of patient demographic data (age, sex, education and behavior using mosquito nets or not). The results showed that out of 164 respondents, 22 patients found Plasmodium parasites. The identified Plasmodium vivax parasites were 20 people (90.9%) while for Plasmodium falciparum there were 2 people (9.1%). Plasmodium parasites identified at the age of 5-11 years (children) were 6 respondents (27.3%), in female sex there were 13 people (59.1%), at the elementary school level there were 15 people (68.2%), the behavior of not using mosquito nets was 12 people (54.5%). From the results of this study it can be concluded that Plasmodium parasites were found and identified that infect people in the work area at the Hanura Village Health Center, Teluk Pandan District, Pesawaran Regency.","PeriodicalId":17791,"journal":{"name":"Jurnal Media Analis Kesehatan","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88244186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Syahida Djasang, Artati Artati, Budirman Budirman, Kalma Kalma, Zulfikar Ali Hasan
Diabetes Mellitus merupakan penyakit metabolik akut dengan tingginya kadar glukosa darah. DM ini juga menghasilkan beberapa komplikasi penyakit seperti nefropati diabetes ditandai dengan albuminuria persisten dan disertai berbagai faktor risiko. Tujuan penelitian ini adalah untuk mengetahui faktor korelasi dengan kejadian mikroalbuminuria pada penderita DM tipe 2. Penelitian ini merupakan penelitian uji korelasi dengan sampel sebanyak 50 orang menggunakan tehnik purposive sampling. Penelitian ini dilaksanakan di Laboratorium Klinik Kimia Farma Makassar pada tanggal 25 Maret – 20 April 2022. Pengumpulan data yakni karakteristik responden dan pengambilan sampel urine untuk mendiagnosis kadar albumin pasien. Analisis data menggunakan pendekatan bivariat dengan uji statistik Korelasi Pearson. Hasil penelitian menunjukkan sebagian besar sampel memiliki kadar mikroalbumin >20 mg/L sebesar 86%, berusia >45 tahun sebesar 86%, mengkonsumsi obat >3 tahun sebesar 70%, memiliki lama diabetes >3 tahun sebesar 74%, dan mengonsumsi jenis obat dosis tinggi sebesar 84%. Kemudian, hasil bivariat menunjukkan terdapat hubungan faktor usia, lama minum obat, lama diabetes, dan jenis obat dengan kejadian Mikroalbumin pada pasien DM tipe 2 dengan masing-masing nilai p value 0,017, 0,000, 0,003 dan 0,037 (p<0,05). Diharapkan kepada masyarakat untuk senantiasa menerapkan pola hidup sehat agar kualitas kehidupan semakin terjamin kedepannya dan diharapkan hasil penelitian ini menjadi bahan rekomendasi dalam pengembangan penelitian selanjutnya.
{"title":"FAKTOR KORELASI KEJADIAN MIKROALBUMINURIA PADA PENDERITA DIABETES MELITUS TIPE 2 DI KLINIK PRATAMA KIMIA FARMA MAKASSAR","authors":"Syahida Djasang, Artati Artati, Budirman Budirman, Kalma Kalma, Zulfikar Ali Hasan","doi":"10.32382/mak.v14i1.3247","DOIUrl":"https://doi.org/10.32382/mak.v14i1.3247","url":null,"abstract":"Diabetes Mellitus merupakan penyakit metabolik akut dengan tingginya kadar glukosa darah. DM ini juga menghasilkan beberapa komplikasi penyakit seperti nefropati diabetes ditandai dengan albuminuria persisten dan disertai berbagai faktor risiko. Tujuan penelitian ini adalah untuk mengetahui faktor korelasi dengan kejadian mikroalbuminuria pada penderita DM tipe 2. Penelitian ini merupakan penelitian uji korelasi dengan sampel sebanyak 50 orang menggunakan tehnik purposive sampling. Penelitian ini dilaksanakan di Laboratorium Klinik Kimia Farma Makassar pada tanggal 25 Maret – 20 April 2022. Pengumpulan data yakni karakteristik responden dan pengambilan sampel urine untuk mendiagnosis kadar albumin pasien. Analisis data menggunakan pendekatan bivariat dengan uji statistik Korelasi Pearson. Hasil penelitian menunjukkan sebagian besar sampel memiliki kadar mikroalbumin >20 mg/L sebesar 86%, berusia >45 tahun sebesar 86%, mengkonsumsi obat >3 tahun sebesar 70%, memiliki lama diabetes >3 tahun sebesar 74%, dan mengonsumsi jenis obat dosis tinggi sebesar 84%. Kemudian, hasil bivariat menunjukkan terdapat hubungan faktor usia, lama minum obat, lama diabetes, dan jenis obat dengan kejadian Mikroalbumin pada pasien DM tipe 2 dengan masing-masing nilai p value 0,017, 0,000, 0,003 dan 0,037 (p<0,05). Diharapkan kepada masyarakat untuk senantiasa menerapkan pola hidup sehat agar kualitas kehidupan semakin terjamin kedepannya dan diharapkan hasil penelitian ini menjadi bahan rekomendasi dalam pengembangan penelitian selanjutnya.","PeriodicalId":17791,"journal":{"name":"Jurnal Media Analis Kesehatan","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135751382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Quality assurance is important to ensure that the quality system runs properly and guarantees the results of laboratory tests, one of which is the inspection of control materials. The control material requirement is to have long stability, blood-like characteristics and low price. From these conditions, commercial control materials have a longer stability than fresh blood but are expensive. In practice, commercial control materials are still used even after the expiry date of the materials has passed. This is because the availability of materials is often delayed, the procurement of materials takes a long time and the price is relatively expensive. Therefore, this study aims to determine the stability of commercial whole blood control stored at 2-8 o C. This research is an experimental analytic study with a cross sectional research design. The sample in this study was commercial whole blood control with 3 levels, namely normal, low abnormally and abnormally high levels which were stored at 2-8 o C and checked every week for 10 weeks. Based on the test results obtained p-value for each parameter at all levels on the parameters of the number of erythrocytes, leukocyte count, hematocrit, hemoglobin and platelet count. Based on the results, it can be concluded that there is no change in the stability of whole blood control with storage for 10 weeks at a temperature of 2-8 o C on the parameters of abnormally low and normal leukocyte count levels, abnormally low and abnormally high hematocrit levels, and all levels on the parameters of leukocyte count, hemoglobin and platelet count. Meanwhile, in the normal level of hematocrit parameters and abnormally high leukocyte count, there is a change in stability.
{"title":"Stabilitas Whole Blood Control Tiga Level Yang Disimpan Pada Suhu 2-8oC Menggunakan Alat Hematology Analyzer","authors":"Adela Caesaria, Anik Handayati, Ayu Puspitasari","doi":"10.26630/jak.v12i1.3492","DOIUrl":"https://doi.org/10.26630/jak.v12i1.3492","url":null,"abstract":"Quality assurance is important to ensure that the quality system runs properly and guarantees the results of laboratory tests, one of which is the inspection of control materials. The control material requirement is to have long stability, blood-like characteristics and low price. From these conditions, commercial control materials have a longer stability than fresh blood but are expensive. In practice, commercial control materials are still used even after the expiry date of the materials has passed. This is because the availability of materials is often delayed, the procurement of materials takes a long time and the price is relatively expensive. Therefore, this study aims to determine the stability of commercial whole blood control stored at 2-8 o C. This research is an experimental analytic study with a cross sectional research design. The sample in this study was commercial whole blood control with 3 levels, namely normal, low abnormally and abnormally high levels which were stored at 2-8 o C and checked every week for 10 weeks. Based on the test results obtained p-value for each parameter at all levels on the parameters of the number of erythrocytes, leukocyte count, hematocrit, hemoglobin and platelet count. Based on the results, it can be concluded that there is no change in the stability of whole blood control with storage for 10 weeks at a temperature of 2-8 o C on the parameters of abnormally low and normal leukocyte count levels, abnormally low and abnormally high hematocrit levels, and all levels on the parameters of leukocyte count, hemoglobin and platelet count. Meanwhile, in the normal level of hematocrit parameters and abnormally high leukocyte count, there is a change in stability.","PeriodicalId":17791,"journal":{"name":"Jurnal Media Analis Kesehatan","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76406455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rahma Wati, Waode Rustiah, Darmawaty Rauf, Kasturi Rais
Minuman beralkohol merupakan minuman yang mengandung etanol dan zat adiktif atau bahan yang bila dikonsumsi dapat menurunkan tingkat kesadaran konsumen dan membuat seseorang ketergantungan. Dampak dari mengonsumsi alkohol secara berlebihan dalam waktu yang lama dapat merusak fungsi hati salah satunya bilirubin total. Bilirubin merupakan pigmen oranye-kuning yang berasal dari sel retikuloendotelial yang memecah hemoglobin dalam sel darah merah dan terbentuk melalui reaksi oksidasi reduksi. Tujuan penelitian ini untuk mengetahui kadar bilirubin total pada pengonsumsi minuman beralkohol. Jenis penelitian bersifat observasi laboratorik dengan metode pengambilan sampel secara purposive sampling yang menggunakan instrumen spektrofotometri. Sampel yang digunakan adalah sampel darah pengonsumsi minuman beralkohol di Desa Lampa Kecamatan Duampanua Kabupaten Pinrang sebanyak 10 sampel. Dari hasil penelitian, didapatkan kadar bilirubin total meningkat sebanyak 5 orang dan hasil normal sebanyak 5 orang. Dengan demikian dapat disimpulkan bahwa dari 10 sampel yang diperiksa, 50% kadar bilirubin total meningkat dan 50 % kadar bilirubin total normal.
{"title":"GAMBARAN PEMERIKSAAN KADAR BILIRUBIN TOTAL PADA PENGONSUMSI MINUMAN BERALKOHOL","authors":"Rahma Wati, Waode Rustiah, Darmawaty Rauf, Kasturi Rais","doi":"10.32382/mak.v14i1.3198","DOIUrl":"https://doi.org/10.32382/mak.v14i1.3198","url":null,"abstract":"Minuman beralkohol merupakan minuman yang mengandung etanol dan zat adiktif atau bahan yang bila dikonsumsi dapat menurunkan tingkat kesadaran konsumen dan membuat seseorang ketergantungan. Dampak dari mengonsumsi alkohol secara berlebihan dalam waktu yang lama dapat merusak fungsi hati salah satunya bilirubin total. Bilirubin merupakan pigmen oranye-kuning yang berasal dari sel retikuloendotelial yang memecah hemoglobin dalam sel darah merah dan terbentuk melalui reaksi oksidasi reduksi. Tujuan penelitian ini untuk mengetahui kadar bilirubin total pada pengonsumsi minuman beralkohol. Jenis penelitian bersifat observasi laboratorik dengan metode pengambilan sampel secara purposive sampling yang menggunakan instrumen spektrofotometri. Sampel yang digunakan adalah sampel darah pengonsumsi minuman beralkohol di Desa Lampa Kecamatan Duampanua Kabupaten Pinrang sebanyak 10 sampel. Dari hasil penelitian, didapatkan kadar bilirubin total meningkat sebanyak 5 orang dan hasil normal sebanyak 5 orang. Dengan demikian dapat disimpulkan bahwa dari 10 sampel yang diperiksa, 50% kadar bilirubin total meningkat dan 50 % kadar bilirubin total normal.","PeriodicalId":17791,"journal":{"name":"Jurnal Media Analis Kesehatan","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135751385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lactic Acid Bacteria (LAB) species grow naturally during the fermentation process of Urutans . This research aims to isolate LAB from Urutan and study its ability to inhibit pathogenic bacteria. According to FAO/WHO, antagonistic activity against enteric pathogenic microbes is one of the criteria for probiotic products. The research was conducted with an exploratory method at the Microbiology Laboratory of the Faculty of Medicine, Udayana University, from February to June 2020. The research samples were five Urutans from Urutan traders in Baturiti District, Tabanan-Bali. LAB was isolated using MRS agar media supplemented with 1% CaCO3. LAB suspects were further confirmed by catalase test and Gram staining. The results showed 326 isolates confirmed as LAB colonies with the characteristics of acidifying growth media, catalase-negative, and Gram-positive rods and cocci. Antibacterial activity test on isolates confirmed as LAB showed that 29 isolates inhibited Escherichia coli and 46 isolates inhibited Staphylococcus aureus . A total of 158 other isolates inhibited the growth of E.coli , and S. aureus , and 93 isolates showed no inhibitory activity on both. 158 LAB isolates that inhibit E.coli and S.aureus could be further studied and developed as probiotic candidates.
{"title":"Eksplorasi Bakteri Asam Laktat dengan Aktivitas Antibakteri dari Urutan sebagai Kandidat Probiotik","authors":"Ni Made Sri Dwijastuti","doi":"10.26630/jak.v12i1.3838","DOIUrl":"https://doi.org/10.26630/jak.v12i1.3838","url":null,"abstract":"Lactic Acid Bacteria (LAB) species grow naturally during the fermentation process of Urutans . This research aims to isolate LAB from Urutan and study its ability to inhibit pathogenic bacteria. According to FAO/WHO, antagonistic activity against enteric pathogenic microbes is one of the criteria for probiotic products. The research was conducted with an exploratory method at the Microbiology Laboratory of the Faculty of Medicine, Udayana University, from February to June 2020. The research samples were five Urutans from Urutan traders in Baturiti District, Tabanan-Bali. LAB was isolated using MRS agar media supplemented with 1% CaCO3. LAB suspects were further confirmed by catalase test and Gram staining. The results showed 326 isolates confirmed as LAB colonies with the characteristics of acidifying growth media, catalase-negative, and Gram-positive rods and cocci. Antibacterial activity test on isolates confirmed as LAB showed that 29 isolates inhibited Escherichia coli and 46 isolates inhibited Staphylococcus aureus . A total of 158 other isolates inhibited the growth of E.coli , and S. aureus , and 93 isolates showed no inhibitory activity on both. 158 LAB isolates that inhibit E.coli and S.aureus could be further studied and developed as probiotic candidates.","PeriodicalId":17791,"journal":{"name":"Jurnal Media Analis Kesehatan","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91168766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sujono Sujono, Sistiyono Sistiyono, Subrata Tri Widada
A bad sample gives an invalid laboratory test result. There are several reasons that can cause a sample to be unfit for examination. The serum that most often causes errors are hemolysis, jaundice and lipemia. Icteric serum is serum that is yellow brown due to hyperbilirubinemia (increased levels of bilirubin in the blood). Icteric serum may cause interference with laboratory tests. Icteric serum can affect measurements at a wavelength of 400-500 nm. Examination by the method of oxidase or peroxidase reactions, such as glucose, cholesterol, triglycerides and uric acid can be disrupted. Potassium Ferricyanide is added to oxidize bilirubin to biliverdin before testing, and can reduce absorption disorders. Potassium ferric cyanide can reduce total bilirubin levels (p=0.000) in icteric serum. There were differences in the results of total cholesterol (p=0.000), triglycerides (p=0.019), glucose (p=0.001), total protein (p=0.011), albumin (p=0.004), globulin (p=0.002)
{"title":"Penggunaan Kalium Feri Sianida Untuk Pengolahan Serum Ikterik","authors":"Sujono Sujono, Sistiyono Sistiyono, Subrata Tri Widada","doi":"10.26630/jak.v12i1.3813","DOIUrl":"https://doi.org/10.26630/jak.v12i1.3813","url":null,"abstract":"A bad sample gives an invalid laboratory test result. There are several reasons that can cause a sample to be unfit for examination. The serum that most often causes errors are hemolysis, jaundice and lipemia. Icteric serum is serum that is yellow brown due to hyperbilirubinemia (increased levels of bilirubin in the blood). Icteric serum may cause interference with laboratory tests. Icteric serum can affect measurements at a wavelength of 400-500 nm. Examination by the method of oxidase or peroxidase reactions, such as glucose, cholesterol, triglycerides and uric acid can be disrupted. Potassium Ferricyanide is added to oxidize bilirubin to biliverdin before testing, and can reduce absorption disorders. Potassium ferric cyanide can reduce total bilirubin levels (p=0.000) in icteric serum. There were differences in the results of total cholesterol (p=0.000), triglycerides (p=0.019), glucose (p=0.001), total protein (p=0.011), albumin (p=0.004), globulin (p=0.002)","PeriodicalId":17791,"journal":{"name":"Jurnal Media Analis Kesehatan","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80620744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Diabetes mellitus is a big public health problem in Indonesia. The length of time of suffering from DM is associated with a decrease in the function of pancreatic beta cells resulting in insulin resistance, which occurs in type 2 diabetes mellitus. Insulin resistance can be determined by measuring copeptin levels. The aim of this study was to determine differences in levels of human CPP protein (copeptin) in diabetes mellitus patients based on length of suffering. This type of research is observational analytic with cross sectional research design. Samples were taken from diabetes mellitus outpatients at Roemani Muhammadiyah Hospital as many as 20 samples, then the samples were examined for levels of human CPP (copeptin) protein using the ELISA (Enzyme Linked Immunosorbent Assay) method. The results showed that the average copeptin level in patients with diabetes mellitus ≤ 3 years was 1054 pg/mL and the average copeptin level in patients with diabetes mellitus > 3 years was 899 pg/mL. Statistical tests using the Independent Sample T Test obtained a significant sig value of 0.027 with a significance level of 0.05, namely 0.027 <0.05 so that it can be concluded that there are differences in copeptin levels in diabetes mellitus patients based on length of suffering. Copeptin levels in patients with diabetes mellitus ≤ 3 years, namely 1054 pg/mL, were higher than copeptin levels in patients with diabetes mellitus > 3 years, namely 899 pg/mL
{"title":"Perbedaan Kadar Protein Human Cpp (Copeptin) Berdasarkan Lama Menderita Pada Pasien Diabetes Melitus","authors":"P. S. Redistia, B. Santosa, Aprilia Indra Kartika","doi":"10.26630/jak.v12i1.3704","DOIUrl":"https://doi.org/10.26630/jak.v12i1.3704","url":null,"abstract":"Diabetes mellitus is a big public health problem in Indonesia. The length of time of suffering from DM is associated with a decrease in the function of pancreatic beta cells resulting in insulin resistance, which occurs in type 2 diabetes mellitus. Insulin resistance can be determined by measuring copeptin levels. The aim of this study was to determine differences in levels of human CPP protein (copeptin) in diabetes mellitus patients based on length of suffering. This type of research is observational analytic with cross sectional research design. Samples were taken from diabetes mellitus outpatients at Roemani Muhammadiyah Hospital as many as 20 samples, then the samples were examined for levels of human CPP (copeptin) protein using the ELISA (Enzyme Linked Immunosorbent Assay) method. The results showed that the average copeptin level in patients with diabetes mellitus ≤ 3 years was 1054 pg/mL and the average copeptin level in patients with diabetes mellitus > 3 years was 899 pg/mL. Statistical tests using the Independent Sample T Test obtained a significant sig value of 0.027 with a significance level of 0.05, namely 0.027 <0.05 so that it can be concluded that there are differences in copeptin levels in diabetes mellitus patients based on length of suffering. Copeptin levels in patients with diabetes mellitus ≤ 3 years, namely 1054 pg/mL, were higher than copeptin levels in patients with diabetes mellitus > 3 years, namely 899 pg/mL","PeriodicalId":17791,"journal":{"name":"Jurnal Media Analis Kesehatan","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88244936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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