Amaryllis (Hippeastrum hybridum) bulbs at the dormant stage were used to investigate contagious virus retention. A DNA fragment containing 612 bp was amplified by reverse-transcription polymerase chain reaction with nerine latent virus (NeLV)-specific primers from amaryllis bulbs and characterized by nucleotide sequencing. Nucleotide sequences of the partial region of the NeLV coat protein gene were determined, and the sequences shared 93.9% to 98.5% identity with other NeLV isolates derived from various Amaryllidaceae species. In the phylogenetic analysis, amaryllis isolates from ‘Alfresco’ and ‘Red Lion’ cultivars used in this study were grouped with other NeLV isolates and distinctively separated from other carlavirus species. In this study, NeLV was found in amaryllis bulbs from the two cultivars. Additional key words: Amaryllidaceae, amaryllis, carlavirus, flower bulb, quarantine
{"title":"Nerine Latent Virus Diagnosed in Hippeastrum Bulbs in Korea","authors":"Eun Song Heo, Sun Hee Choi","doi":"10.7235/HORT.20210045","DOIUrl":"https://doi.org/10.7235/HORT.20210045","url":null,"abstract":"Amaryllis (Hippeastrum hybridum) bulbs at the dormant stage were used to investigate contagious virus retention. A DNA fragment containing 612 bp was amplified by reverse-transcription polymerase chain reaction with nerine latent virus (NeLV)-specific primers from amaryllis bulbs and characterized by nucleotide sequencing. Nucleotide sequences of the partial region of the NeLV coat protein gene were determined, and the sequences shared 93.9% to 98.5% identity with other NeLV isolates derived from various Amaryllidaceae species. In the phylogenetic analysis, amaryllis isolates from ‘Alfresco’ and ‘Red Lion’ cultivars used in this study were grouped with other NeLV isolates and distinctively separated from other carlavirus species. In this study, NeLV was found in amaryllis bulbs from the two cultivars. Additional key words: Amaryllidaceae, amaryllis, carlavirus, flower bulb, quarantine","PeriodicalId":17858,"journal":{"name":"Korean Journal of Horticultural Science & Technology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41327140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study was performed to identify the effects of changes in irrigation amounts on the ‘fruiting period’ and of irrigation electrical conductivity (EC) level by the growth period on the growth and fruit properties of melon in hydroponics, using coir substrate. The irrigation amount was regulated based on the drainage rate (%), which was 30% after transplanting. The irrigation amount during the ‘fruiting period’ was regulated to the drainage rate of 10% and 30% for the two consecutive treatments of the irrigation level, and after 40 days of fruiting, the drainage rate was maintained at an equal level of 10%. The irrigation EC level was set to three treatments; low level (1.0-1.5-1.8 dS·m -1 ), middle level (1.5-1.8-2.0 dS·m -1 ), and high level (1.8-1.8-2.3 dS·m -1 ), according to the three growth periods: flowering period, fruiting period, and 40 days after fruiting. For the changes in drainage EC, a low irrigation EC level indicated a low drainage EC level, while a lower value was shown by the 30% drainage treatment with a higher irrigation amount than by the 10% drainage treatment. The results show that leaf length, leaf width, and leaf area were the highest at 30% drainage and the irrigation EC level on the fruiting period was high, but the middle level EC showed a similar pattern without a significant difference. Thus, for the EC level, the middle level (1.5-1.8-2.0 dS·m -1 ) appears to be most suitable. The irrigation amount had more influence on fruit weight, fruit height, fruit width, and fruit shape than the EC level. Meanwhile, the 30% drainage rate group showed higher values. An increase in the irrigation EC level by growth period was found to have increased the fresh and dry weights of the plant, although the fruit sugar content showed no significant difference. The findings of this study suggest that for the hydroponics of the net melon ‘PMR Worldstar’ using coir substrate, a 30% drainage rate was more favorable for melon fruit
{"title":"Effects of Irrigation Amount on Fruiting Period and EC Level by Growth Period on Growth and Quality of Melon (Cucumis melo L.) Using Coir Substrate Hydroponics During Autumn Cultivation","authors":"M. Lim, S. Choi, G. Choi, So Hui Kim, H. Jeong","doi":"10.7235/HORT.20210040","DOIUrl":"https://doi.org/10.7235/HORT.20210040","url":null,"abstract":"This study was performed to identify the effects of changes in irrigation amounts on the ‘fruiting period’ and of irrigation electrical conductivity (EC) level by the growth period on the growth and fruit properties of melon in hydroponics, using coir substrate. The irrigation amount was regulated based on the drainage rate (%), which was 30% after transplanting. The irrigation amount during the ‘fruiting period’ was regulated to the drainage rate of 10% and 30% for the two consecutive treatments of the irrigation level, and after 40 days of fruiting, the drainage rate was maintained at an equal level of 10%. The irrigation EC level was set to three treatments; low level (1.0-1.5-1.8 dS·m -1 ), middle level (1.5-1.8-2.0 dS·m -1 ), and high level (1.8-1.8-2.3 dS·m -1 ), according to the three growth periods: flowering period, fruiting period, and 40 days after fruiting. For the changes in drainage EC, a low irrigation EC level indicated a low drainage EC level, while a lower value was shown by the 30% drainage treatment with a higher irrigation amount than by the 10% drainage treatment. The results show that leaf length, leaf width, and leaf area were the highest at 30% drainage and the irrigation EC level on the fruiting period was high, but the middle level EC showed a similar pattern without a significant difference. Thus, for the EC level, the middle level (1.5-1.8-2.0 dS·m -1 ) appears to be most suitable. The irrigation amount had more influence on fruit weight, fruit height, fruit width, and fruit shape than the EC level. Meanwhile, the 30% drainage rate group showed higher values. An increase in the irrigation EC level by growth period was found to have increased the fresh and dry weights of the plant, although the fruit sugar content showed no significant difference. The findings of this study suggest that for the hydroponics of the net melon ‘PMR Worldstar’ using coir substrate, a 30% drainage rate was more favorable for melon fruit","PeriodicalId":17858,"journal":{"name":"Korean Journal of Horticultural Science & Technology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46272983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The primary purpose of this study was to examine the effects of virus infection in Phalaenopsis cultivated across the country. RT-PCR analysis was used to detect two major viruses in orchid: CymMV and ORSV. The virus infection rate of in vitro plantlets from 45 cultivars was 83.6%, and the rate of potted plants from 21 cultivars was 92.5%. For CymMV, single infection in in vitro plantlets was 34.3%, and for ORSV, it was 11.4%. In the case of potted plants, single infection with CymMV was 57.0%, and ORSV alone was not detected. For double infection with both viruses, in vitro plants accounted for 37.9% and potted plants for 35.5%. No infection with both viruses was found in in vitro plantlets with ‘V3’ imported from Taiwan. Plants infected with CymMV showed chlorotic and necrotic spots on leaves and color break in petals and sepals of flowers, and those infected with both CymMV and ORSV featured yellow striped or mosaic symptoms. Additional key words: CymMV, DN method, in vitro plantlets, ORSV, potted plants, RT-PCR
{"title":"Detection of Cymbidium mosaic virus and Odontoglossum ringspot virus in Phalaenopsis in Korea","authors":"D. Jeong, K. Hong, Kyeung-il Park","doi":"10.7235/HORT.20210046","DOIUrl":"https://doi.org/10.7235/HORT.20210046","url":null,"abstract":"The primary purpose of this study was to examine the effects of virus infection in Phalaenopsis cultivated across the country. RT-PCR analysis was used to detect two major viruses in orchid: CymMV and ORSV. The virus infection rate of in vitro plantlets from 45 cultivars was 83.6%, and the rate of potted plants from 21 cultivars was 92.5%. For CymMV, single infection in in vitro plantlets was 34.3%, and for ORSV, it was 11.4%. In the case of potted plants, single infection with CymMV was 57.0%, and ORSV alone was not detected. For double infection with both viruses, in vitro plants accounted for 37.9% and potted plants for 35.5%. No infection with both viruses was found in in vitro plantlets with ‘V3’ imported from Taiwan. Plants infected with CymMV showed chlorotic and necrotic spots on leaves and color break in petals and sepals of flowers, and those infected with both CymMV and ORSV featured yellow striped or mosaic symptoms. Additional key words: CymMV, DN method, in vitro plantlets, ORSV, potted plants, RT-PCR","PeriodicalId":17858,"journal":{"name":"Korean Journal of Horticultural Science & Technology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43075993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Yılmaz, Hasan Pınar Kaya, H. Pınar, F. Hancı, A. Uzun
Information on the genetic diversity of local melon (Cucumis melo L.) genotypes is important for breeding and germplasm conservation efforts. This study was to characterize the morphological and molecular features of local melon (Cucumis melo L.) accessions collected from different regions of northern Cyprus and Turkey. We studied 32 melon accessions, comprising 19 accessions collected from different areas in northern Cyprus, 4 hybrid cultivars, and 9 local accessions grown in Turkey. Their molecular characteristics were determined using sequence-related amplified polymorphism (SRAP) markers, and their morphological characteristics were determined according to criteria from the International Union for the Protection of New Varieties of Plants and Institute of Plant Genetic Resources. Our morphology data classified the 32 melon accessions into two distinct groups, and our molecular data identified 56 total bands were obtained using 11 SRAP primer combinations, and 11 of them were polymorphic at molecular characterization. The polymorphism rate was 0.69-0.96%. Additional key words: genetic diversity, genetic resources, local accessions, morphological traits, principal component analysis
{"title":"Detailed Morphological and Molecular Characterizations of Melon (Cucumis melo L.) Accessions Collected from Northern Cyprus and Turkey","authors":"N. Yılmaz, Hasan Pınar Kaya, H. Pınar, F. Hancı, A. Uzun","doi":"10.7235/HORT.20210042","DOIUrl":"https://doi.org/10.7235/HORT.20210042","url":null,"abstract":"Information on the genetic diversity of local melon (Cucumis melo L.) genotypes is important for breeding and germplasm conservation efforts. This study was to characterize the morphological and molecular features of local melon (Cucumis melo L.) accessions collected from different regions of northern Cyprus and Turkey. We studied 32 melon accessions, comprising 19 accessions collected from different areas in northern Cyprus, 4 hybrid cultivars, and 9 local accessions grown in Turkey. Their molecular characteristics were determined using sequence-related amplified polymorphism (SRAP) markers, and their morphological characteristics were determined according to criteria from the International Union for the Protection of New Varieties of Plants and Institute of Plant Genetic Resources. Our morphology data classified the 32 melon accessions into two distinct groups, and our molecular data identified 56 total bands were obtained using 11 SRAP primer combinations, and 11 of them were polymorphic at molecular characterization. The polymorphism rate was 0.69-0.96%. Additional key words: genetic diversity, genetic resources, local accessions, morphological traits, principal component analysis","PeriodicalId":17858,"journal":{"name":"Korean Journal of Horticultural Science & Technology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43458672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Nasir, Muqarab Ali, S. Ayyub, B. Akram, R. Hussain, Zahid Mustafa, Hafiz Muhammad Tayyab Khan
Soil salinity harmfully effect bell pepper production being salt sensitive crop. The aim of this study was to compare capsicum (bell-pepper) cultivars for their ability to tolerate salt stress. Electrical conductivity of different levels i.e., control (no sodium chloride), 1.5, 3.0, 4.5, and 6.0 dS m-1, was developed by using NaCl, after 30 days of seedling emergence of six cultivars (Yolo Wonder, California Wonder, Cop-amber Hybrid, F1 Pangs No. 206, Sweet Pepper F1, Kaka F1). These cultivars were evaluated based on morphological (shoot and root length, plant fresh and dry biomass), physiological (chlorophyll content) and biochemical attributes (nitrogen concentration, and protein contents). Minimum chlorophyll contents (SPAD units) in Kaka F1 (16.77 SPAD units) at 1.5 dSm-1. However, maximum value of chlorophyll contents was observed in Sweet Pepper F1 (49.23 SPAD units) at control followed by “Yolo Wonder” (41.53 SPAD units) at control. Maximum dry biomass was found in California Wonder (0.468 g) at 1.5 dSm-1, while minimum value of dry biomass (g) was observed in Kaka F1 (0.095 g) at 4.5 dSm-1. Six cultivars of capsicum were categorized into three groups. Salt tolerant cultivars included “California Wonder” followed by “Yolo Wonder”. “Kaka F1” was proved most salt sensitive and all others were moderately salt tolerant.
{"title":"Evaluation of Capsicum annum L. genotypes against salinity induced by NaCl","authors":"M. Nasir, Muqarab Ali, S. Ayyub, B. Akram, R. Hussain, Zahid Mustafa, Hafiz Muhammad Tayyab Khan","doi":"10.46653/jhst2142062","DOIUrl":"https://doi.org/10.46653/jhst2142062","url":null,"abstract":"Soil salinity harmfully effect bell pepper production being salt sensitive crop. The aim of this study was to compare capsicum (bell-pepper) cultivars for their ability to tolerate salt stress. Electrical conductivity of different levels i.e., control (no sodium chloride), 1.5, 3.0, 4.5, and 6.0 dS m-1, was developed by using NaCl, after 30 days of seedling emergence of six cultivars (Yolo Wonder, California Wonder, Cop-amber Hybrid, F1 Pangs No. 206, Sweet Pepper F1, Kaka F1). These cultivars were evaluated based on morphological (shoot and root length, plant fresh and dry biomass), physiological (chlorophyll content) and biochemical attributes (nitrogen concentration, and protein contents). Minimum chlorophyll contents (SPAD units) in Kaka F1 (16.77 SPAD units) at 1.5 dSm-1. However, maximum value of chlorophyll contents was observed in Sweet Pepper F1 (49.23 SPAD units) at control followed by “Yolo Wonder” (41.53 SPAD units) at control. Maximum dry biomass was found in California Wonder (0.468 g) at 1.5 dSm-1, while minimum value of dry biomass (g) was observed in Kaka F1 (0.095 g) at 4.5 dSm-1. Six cultivars of capsicum were categorized into three groups. Salt tolerant cultivars included “California Wonder” followed by “Yolo Wonder”. “Kaka F1” was proved most salt sensitive and all others were moderately salt tolerant.","PeriodicalId":17858,"journal":{"name":"Korean Journal of Horticultural Science & Technology","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88129783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Munir Ahmed, S. Ullah, K. Razzaq, I. A. Rajwana, Gulzar Akhtar, Ambreen Naz, M. Amin, M. Khalid, S. Khalid
Apricot is a highly nutritive stone fruit which ripens quickly after harvest and exhibits rapid fruit quality deterioration at ambient conditions. The current research work was executed to evaluate the effect of pre-harvest application of oxalic acid (OA); (0, 0.5, 1, and 2mM) on fruit quality of ‘Red Flesh’ apricot during fruit ripening at shelf under ambient conditions (25±1 °C; 60-65% RH). Fruit size, average fruit weight, fruit juice percentage was determined at harvest. While, fruit weight loss, fruit colour, fruit firmness, titratable acidity (TA), soluble solid contents (SSC), ratio of SSC to TA, ascorbic acid, total phenolics content (TPC), anti-oxidative scavenging activity (ASA), superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) enzymes activity were determined during fruit ripening. Fruit sensory attributes (fruit pulp colour, fruit taste, fruit flavour and overall acceptability were determined at fruit ripening. Pre-harvest application of 2mM-OA was found the most effective in improving fruit size, average fruit weight, juice percentage at harvest, about 5%, 6% and 15% higher than control, respectively. Additionally, pre-harvest application of 2mM-OA retained higher fruit firmness, ascorbic acid, TPC, ASA and activities of POD, CAT enzymes in apricot fruit during fruit ripening at ambient conditions. On day-5 of fruit ripening 2mM-OA-treated apricot fruit exhibited about 28%, 20%, 17%, 7%, 9% and 23% higher fruit firmness, ascorbic acid, TPC, ASA and activities of POD, CAT enzymes, respectively as compared to control. Moreover, significant lower fruit weight loss (35%), SSC (20%) and SSC:TA ratio (30%) were exhibited by the apricot fruit treated with 2mM-OA than unsprayed apricot fruit. However, fruit treated with 1 mM-OA exhibited better fruit sensory attributes compared to other treatments at fruit ripening. Conclusively, pre-harvest application of 2 mM-OA improved the fruit size, delayed fruit ripening, and retained higher fruit antioxidants of apricot at ambient conditions.
{"title":"Pre-Harvest Oxalic Acid Application Improves Fruit Size at Harvest, Physico-Chemical and Sensory Attributes of ‘Red Flesh’ Apricot During Fruit Ripening","authors":"Munir Ahmed, S. Ullah, K. Razzaq, I. A. Rajwana, Gulzar Akhtar, Ambreen Naz, M. Amin, M. Khalid, S. Khalid","doi":"10.46653/jhst2142048","DOIUrl":"https://doi.org/10.46653/jhst2142048","url":null,"abstract":"Apricot is a highly nutritive stone fruit which ripens quickly after harvest and exhibits rapid fruit quality deterioration at ambient conditions. The current research work was executed to evaluate the effect of pre-harvest application of oxalic acid (OA); (0, 0.5, 1, and 2mM) on fruit quality of ‘Red Flesh’ apricot during fruit ripening at shelf under ambient conditions (25±1 °C; 60-65% RH). Fruit size, average fruit weight, fruit juice percentage was determined at harvest. While, fruit weight loss, fruit colour, fruit firmness, titratable acidity (TA), soluble solid contents (SSC), ratio of SSC to TA, ascorbic acid, total phenolics content (TPC), anti-oxidative scavenging activity (ASA), superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) enzymes activity were determined during fruit ripening. Fruit sensory attributes (fruit pulp colour, fruit taste, fruit flavour and overall acceptability were determined at fruit ripening. Pre-harvest application of 2mM-OA was found the most effective in improving fruit size, average fruit weight, juice percentage at harvest, about 5%, 6% and 15% higher than control, respectively. Additionally, pre-harvest application of 2mM-OA retained higher fruit firmness, ascorbic acid, TPC, ASA and activities of POD, CAT enzymes in apricot fruit during fruit ripening at ambient conditions. On day-5 of fruit ripening 2mM-OA-treated apricot fruit exhibited about 28%, 20%, 17%, 7%, 9% and 23% higher fruit firmness, ascorbic acid, TPC, ASA and activities of POD, CAT enzymes, respectively as compared to control. Moreover, significant lower fruit weight loss (35%), SSC (20%) and SSC:TA ratio (30%) were exhibited by the apricot fruit treated with 2mM-OA than unsprayed apricot fruit. However, fruit treated with 1 mM-OA exhibited better fruit sensory attributes compared to other treatments at fruit ripening. Conclusively, pre-harvest application of 2 mM-OA improved the fruit size, delayed fruit ripening, and retained higher fruit antioxidants of apricot at ambient conditions.","PeriodicalId":17858,"journal":{"name":"Korean Journal of Horticultural Science & Technology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77289774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hafiz Farooq Anwar, H. Shah, A. Waheed, Mudassar Anwar Butt, Hafiz Zafar-ul-Qasim, Asim Bari
Lily (Lilium L. hybrids) is sub-tropical cut flower that can be grown in open place or in green house and has consumer demand in local as well as in offshore markets. Short vase life, postharvest decay of flowering petals, activities of oxidative enzymes, malondialdehyde contents, electrolyte leakage and low water ratio are the main problems in marketing of cut lilies. This study was carried out to investigate the effect of oxalic acid (OA) treatments on vase life (12 days) and postharvest quality of ‘Mero Star’ cut lily flowers. The flowering stems were placed in 2, 4 and 6% aqueous solution of OA for 12 days at 20±2 °C with 70±5% relative humidity. Flowers placed in 6% OA solution exhibited longest vase life and flower diameter with higher relative water content and increased fresh weight and reduced flower decay. Reduced malondialdehyde contents and electrolyte leakage with higher activities of catalase, superoxide dismutase and peroxidase enzymes were also noted in 6% OA-treated flowers. In conclusion, exogenous postharvest application of 6% OA extended vase life and maintained postharvest quality of cut flowers by reducing electrolyte leakage and malondialdehyde contents with higher antioxidative enzymes activities.
{"title":"Effect of oxalic acid on vase life and antioxidative activities of ‘Mero Star’ cut lily flowers","authors":"Hafiz Farooq Anwar, H. Shah, A. Waheed, Mudassar Anwar Butt, Hafiz Zafar-ul-Qasim, Asim Bari","doi":"10.46653/jhst2142056","DOIUrl":"https://doi.org/10.46653/jhst2142056","url":null,"abstract":"Lily (Lilium L. hybrids) is sub-tropical cut flower that can be grown in open place or in green house and has consumer demand in local as well as in offshore markets. Short vase life, postharvest decay of flowering petals, activities of oxidative enzymes, malondialdehyde contents, electrolyte leakage and low water ratio are the main problems in marketing of cut lilies. This study was carried out to investigate the effect of oxalic acid (OA) treatments on vase life (12 days) and postharvest quality of ‘Mero Star’ cut lily flowers. The flowering stems were placed in 2, 4 and 6% aqueous solution of OA for 12 days at 20±2 °C with 70±5% relative humidity. Flowers placed in 6% OA solution exhibited longest vase life and flower diameter with higher relative water content and increased fresh weight and reduced flower decay. Reduced malondialdehyde contents and electrolyte leakage with higher activities of catalase, superoxide dismutase and peroxidase enzymes were also noted in 6% OA-treated flowers. In conclusion, exogenous postharvest application of 6% OA extended vase life and maintained postharvest quality of cut flowers by reducing electrolyte leakage and malondialdehyde contents with higher antioxidative enzymes activities.","PeriodicalId":17858,"journal":{"name":"Korean Journal of Horticultural Science & Technology","volume":"512 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75234286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nida Akram, M. Hasan, R. Rehman, R. Ahmad, Z. Ahmed, U. Khan, F. Hayat
Chilling injury is the critical issue in sweet pepper fruit under low temperature storage. Present work was aimed to evaluate the effect of different concentration of methyl salicylate (MS) and L-arginine (Arg) on chilling injury and overall quality of sweet pepper fruits cv. ‘Winner’. The treatments were T1 = Control, T2 = 0.01mM MS, T3 = 0.05 mM MS, T4 = 1mM Arg, T5 = 1.5mM Arg and T6 = 0.01mM MS+1.5mM Arg. After respective treatment (for 10 min) fruits were kept at 5 ± 1 ◦C with 85-90 % RH for 28+2 days of storage. Physical, biochemical, and phytochemical parameters were studied at 7 days interval followed by two days of reconditioning at ambient conditions (25 ± 2 °C). On last removal (28+2days), sweet pepper fruits treated with combined MS and Arg treatment (T6) showed highly significant results in lower fruit weight loss (8.3%), maintained fruit colour (0.3 score) and firmness (13.4N), and reduced wrinkling (2 score), disease incidence (0.4 score), ion leakage (45.4%), alleviated chilling injury (1.7 score), retained total antioxidants (49.1%) and total phenolic content (74.4 mg 100 g-1 GAE FW) as compared to control. In addition, the ascorbic acid content was observed higher in all treatments in comparison with untreated control fruits. In conclusion, MS and Arg combine treatment improved storage potential with reduced chilling injury by maintaining higher total phenolic concentrations, ascorbic acid content and total antioxidants in terms of DPPH radical scavenging activities, and markedly maintained overall quality of sweet pepper under cold storage condition at 5 °C for 28 days.
{"title":"Combined application of methyl salicylate and L-arginine alleviates chilling injury, potentiates antioxidant system and maintains quality of sweet pepper (Capsicum Annum L.) fruits cv. ‘Winner’","authors":"Nida Akram, M. Hasan, R. Rehman, R. Ahmad, Z. Ahmed, U. Khan, F. Hayat","doi":"10.46653/jhst2142068","DOIUrl":"https://doi.org/10.46653/jhst2142068","url":null,"abstract":"Chilling injury is the critical issue in sweet pepper fruit under low temperature storage. Present work was aimed to evaluate the effect of different concentration of methyl salicylate (MS) and L-arginine (Arg) on chilling injury and overall quality of sweet pepper fruits cv. ‘Winner’. The treatments were T1 = Control, T2 = 0.01mM MS, T3 = 0.05 mM MS, T4 = 1mM Arg, T5 = 1.5mM Arg and T6 = 0.01mM MS+1.5mM Arg. After respective treatment (for 10 min) fruits were kept at 5 ± 1 ◦C with 85-90 % RH for 28+2 days of storage. Physical, biochemical, and phytochemical parameters were studied at 7 days interval followed by two days of reconditioning at ambient conditions (25 ± 2 °C). On last removal (28+2days), sweet pepper fruits treated with combined MS and Arg treatment (T6) showed highly significant results in lower fruit weight loss (8.3%), maintained fruit colour (0.3 score) and firmness (13.4N), and reduced wrinkling (2 score), disease incidence (0.4 score), ion leakage (45.4%), alleviated chilling injury (1.7 score), retained total antioxidants (49.1%) and total phenolic content (74.4 mg 100 g-1 GAE FW) as compared to control. In addition, the ascorbic acid content was observed higher in all treatments in comparison with untreated control fruits. In conclusion, MS and Arg combine treatment improved storage potential with reduced chilling injury by maintaining higher total phenolic concentrations, ascorbic acid content and total antioxidants in terms of DPPH radical scavenging activities, and markedly maintained overall quality of sweet pepper under cold storage condition at 5 °C for 28 days.","PeriodicalId":17858,"journal":{"name":"Korean Journal of Horticultural Science & Technology","volume":"73 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84235926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Micronutrients applications are effective for better crop production in calcareous soils because these soils are usually deficit in iron (Fe), zinc (Zn) and boron (B). In Pakistan, most of soils are calcareous in nature. When tuberose is grown in such soils as a cut flower, its production is negatively affected. Therefore, a study was aimed to evaluate the effects of micronutrients as foliar sprays on cut tuberose production in calcareous soil. Micronutrients (Fe, Zn and B) alone and in combinations were sprayed on the plants after 60, 90 and 120 days of planting. The mixture of all these three micronutrients increased the plant height (95.77 cm), chlorophyll content (38.13 SPAD), number of leaves (79.63), leaf length (44.73 cm), fresh (111.64 g) and dry (16.16 g) plant weights, root length (15.13 cm), number of stalks (3.73), stalk length (79.03 cm), spike length (22 cm), number of florets (51.67), floret fresh weight (11.85 g), leaf Zn concentration (53.6 mg/g) and vase life (8.4 days). Fe + Zn enhanced the number of leaves (76.60), leaf length (45.83 cm), root length (15.05 cm), spike length (22.33 cm), and leaf Fe (128.18 mg/g) and Zn concentrations (55.02 mg/g). The Fe spray increased the leaf length (44.10 cm), days to flower initiation (142.47 days) and leaf Fe concentration (130.75 mg/g) in tuberose plants. Application of Zn improved the leaf length (45.87 cm) and diameter (1.32 cm), root (15.03 cm), spike (21.77 cm) and floret lengths (4.74 cm), floret dry weight (1.49 g) and leaf Zn concentration (57.5 mg/g). Foliar spray of B increased the B concentration (21.1 mg/g) in tuberose leaves. It is concluded that foliar application of micronutrients alone and in combinations improved the plant growth, flowering, leaf minerals concentrations and vase life of tuberose spikes. However, mixture of Fe, Zn and B was more effective as compared to other treatments. Moreover, current study encourages the foliar application of micronutrients in tuberose when grown in calcareous soils.
{"title":"Foliar application of micronutrients enhances growth, flowering, minerals absorption and postharvest life of tuberose (Polianthes tuberosa L.) in calcareous soil","authors":"Sanaullah Mudassir, R. Ahmad, M. A. Anjum","doi":"10.46653/jhst2142041","DOIUrl":"https://doi.org/10.46653/jhst2142041","url":null,"abstract":"Micronutrients applications are effective for better crop production in calcareous soils because these soils are usually deficit in iron (Fe), zinc (Zn) and boron (B). In Pakistan, most of soils are calcareous in nature. When tuberose is grown in such soils as a cut flower, its production is negatively affected. Therefore, a study was aimed to evaluate the effects of micronutrients as foliar sprays on cut tuberose production in calcareous soil. Micronutrients (Fe, Zn and B) alone and in combinations were sprayed on the plants after 60, 90 and 120 days of planting. The mixture of all these three micronutrients increased the plant height (95.77 cm), chlorophyll content (38.13 SPAD), number of leaves (79.63), leaf length (44.73 cm), fresh (111.64 g) and dry (16.16 g) plant weights, root length (15.13 cm), number of stalks (3.73), stalk length (79.03 cm), spike length (22 cm), number of florets (51.67), floret fresh weight (11.85 g), leaf Zn concentration (53.6 mg/g) and vase life (8.4 days). Fe + Zn enhanced the number of leaves (76.60), leaf length (45.83 cm), root length (15.05 cm), spike length (22.33 cm), and leaf Fe (128.18 mg/g) and Zn concentrations (55.02 mg/g). The Fe spray increased the leaf length (44.10 cm), days to flower initiation (142.47 days) and leaf Fe concentration (130.75 mg/g) in tuberose plants. Application of Zn improved the leaf length (45.87 cm) and diameter (1.32 cm), root (15.03 cm), spike (21.77 cm) and floret lengths (4.74 cm), floret dry weight (1.49 g) and leaf Zn concentration (57.5 mg/g). Foliar spray of B increased the B concentration (21.1 mg/g) in tuberose leaves. It is concluded that foliar application of micronutrients alone and in combinations improved the plant growth, flowering, leaf minerals concentrations and vase life of tuberose spikes. However, mixture of Fe, Zn and B was more effective as compared to other treatments. Moreover, current study encourages the foliar application of micronutrients in tuberose when grown in calcareous soils.","PeriodicalId":17858,"journal":{"name":"Korean Journal of Horticultural Science & Technology","volume":"77 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81206552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Eun-Gyeong Kim, G. Ahn, Girim Park, Beung-Gu Son, Y. Choi, Jum-soon Kang, Youngjae Lee, B. I. Je, Younghoon Park
We analyzed the major fruit traits and transcriptomes between a late-ripening cultivar ‘Fuyu’ and an early-ripening cultivar ‘Soshu’ of pollination constant non-astringent persimmon (Diospyros kaki Thunb.) at different developmental stages. Sugar concentration and fruit peel color were measured every 3 weeks, until 18 weeks after full bloom (WAB) for ‘Soshu’ and 24 WAB for ‘Fuyu’. For the sugar concentration in fruit, sucrose levels began to decline 9 weeks earlier in ‘Soshu’. Fruit peel coloring progressed faster in ‘Soshu’, and differences in color between the cultivars were most notable at 18 WAB. Transcriptome analysis was carried out for fruit flesh every 3 weeks from 9-18 WAB. Gene ontology term analysis of the transcriptome indicated that catalytic and transport genes involved in metabolism and growth in cells and cellular organelles were mainly expressed, while in KOG and KEGG analysis, the majority of expressed genes were related to signal transduction, nuclear structure, and starch and sucrose metabolism. Differentially expressed genes were analyzed from two sets of samples: set I, in which fruits sampled at 12, 15, and 18 WAB were compared as the treatment group to the sample collected at 9 WAB as the control for each cultivar; and set II, in which fruit samples of ‘Soshu’ were compared as the treatment group to ‘Fuyu’ as the control at each WAB. For the DEGs of set I, containing three comparison combinations, the number of up-regulated genes in the treatment sample was highest at 18 WAB in ‘Fuyu’ and at 15 WAB in ‘Soshu’. For the DEGs of set II, the number of down-regulated genes and the number of up-regulated genes were higher in ‘Soshu’ compared to ‘Fuyu’ at 12 WAB and 15 WAB, respectively. We then compared the expression patterns of fruit trait-related genes with the phenotypic changes in traits. Our results indicated that UGPase-1 and AGPase-1, involved in carbohydrate metabolism, and CRTISO and PDS, involved in carotenoid biosynthesis, may induce early fruit ripening in ‘Soshu’. In addition,
我们分析了授粉不涩柿子（Diospyros kaki Thunb.）晚熟品种“扶余”和早熟品种“苏树”在不同发育阶段的主要果实性状和转录组。每3周测量一次糖浓度和果皮颜色，直到‘Soshu’开花后18周（WAB）和‘Fuyu’开花后24周。对于果实中的糖浓度，“苏树”的蔗糖水平早于9周开始下降。“苏树”果皮着色进展较快，品种间的颜色差异在18WAB时最为显著。从9-18 WAB开始，每3周对果肉进行一次转录组分析。转录组的基因本体论术语分析表明，参与细胞和细胞器代谢和生长的催化和转运基因主要表达，而在KOG和KEGG分析中，大多数表达的基因与信号转导、核结构以及淀粉和蔗糖代谢有关。从两组样品中分析差异表达的基因：组I，其中在12、15和18WAB处取样的水果作为处理组与在9WAB处收集的样品作为每个品种的对照进行比较；和第二组，其中在每个WAB将“Soshu”果实样品作为处理组与“Fuyu”作为对照进行比较。对于包含三个比较组合的集合I的DEG，处理样本中上调基因的数量在“扶余”的18WAB和“苏蜀”的15WAB处最高。对于集合II的DEG，在12WAB和15WAB时，“Soshu”的下调基因数量和上调基因数量分别高于“Fuyu”。然后，我们将果实性状相关基因的表达模式与性状的表型变化进行了比较。我们的研究结果表明，参与碳水化合物代谢的UGPase-1和AGPase-1，以及参与类胡萝卜素生物合成的CRTISO和PDS，可能会诱导“苏树”果实早熟。此外
{"title":"Traits and Gene Expression Analysis of Early and Late Ripening Pollination Constant Non-Astringent Persimmon Fruits at Different Developmental Phases","authors":"Eun-Gyeong Kim, G. Ahn, Girim Park, Beung-Gu Son, Y. Choi, Jum-soon Kang, Youngjae Lee, B. I. Je, Younghoon Park","doi":"10.7235/HORT.20210035","DOIUrl":"https://doi.org/10.7235/HORT.20210035","url":null,"abstract":"We analyzed the major fruit traits and transcriptomes between a late-ripening cultivar ‘Fuyu’ and an early-ripening cultivar ‘Soshu’ of pollination constant non-astringent persimmon (Diospyros kaki Thunb.) at different developmental stages. Sugar concentration and fruit peel color were measured every 3 weeks, until 18 weeks after full bloom (WAB) for ‘Soshu’ and 24 WAB for ‘Fuyu’. For the sugar concentration in fruit, sucrose levels began to decline 9 weeks earlier in ‘Soshu’. Fruit peel coloring progressed faster in ‘Soshu’, and differences in color between the cultivars were most notable at 18 WAB. Transcriptome analysis was carried out for fruit flesh every 3 weeks from 9-18 WAB. Gene ontology term analysis of the transcriptome indicated that catalytic and transport genes involved in metabolism and growth in cells and cellular organelles were mainly expressed, while in KOG and KEGG analysis, the majority of expressed genes were related to signal transduction, nuclear structure, and starch and sucrose metabolism. Differentially expressed genes were analyzed from two sets of samples: set I, in which fruits sampled at 12, 15, and 18 WAB were compared as the treatment group to the sample collected at 9 WAB as the control for each cultivar; and set II, in which fruit samples of ‘Soshu’ were compared as the treatment group to ‘Fuyu’ as the control at each WAB. For the DEGs of set I, containing three comparison combinations, the number of up-regulated genes in the treatment sample was highest at 18 WAB in ‘Fuyu’ and at 15 WAB in ‘Soshu’. For the DEGs of set II, the number of down-regulated genes and the number of up-regulated genes were higher in ‘Soshu’ compared to ‘Fuyu’ at 12 WAB and 15 WAB, respectively. We then compared the expression patterns of fruit trait-related genes with the phenotypic changes in traits. Our results indicated that UGPase-1 and AGPase-1, involved in carbohydrate metabolism, and CRTISO and PDS, involved in carotenoid biosynthesis, may induce early fruit ripening in ‘Soshu’. In addition,","PeriodicalId":17858,"journal":{"name":"Korean Journal of Horticultural Science & Technology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43602467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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