Ksenia Achkasova, Liudmila Kukhnina, Alexander Moiseev, Elena Kiseleva, Alexandra Bogomolova, Maria Loginova, Natalia Gladkova
Detection of radiation-induced changes of the brain white matter is important for brain neoplasms repeated surgery. We investigated the influence of irradiation on the scattering properties of the white matter using optical coherence tomography (OCT). Healthy Wistar rats undergone the irradiation of the brain right hemisphere. At seven time points from the irradiation procedure (2–14 weeks), an ex vivo OCT study was performed with subsequent calculation of attenuation coefficient values in the corpus callosum followed by immunohistochemical analysis. As a result, we discovered acute and early-delayed changes characterized by the edema of different severity, accompanied by a statistically significant decrease in attenuation coefficient values. In particular, these changes were found at 2 weeks after irradiation in the irradiated hemisphere, while at 6- and 12-week time points they affected both irradiated and contralateral hemisphere. Thus, radiation-induced changes occurring in white matter during the first 3 months after irradiation can be detected by OCT.
{"title":"Detection of acute and early-delayed radiation-induced changes in the white matter of the rat brain based on numerical processing of optical coherence tomography data","authors":"Ksenia Achkasova, Liudmila Kukhnina, Alexander Moiseev, Elena Kiseleva, Alexandra Bogomolova, Maria Loginova, Natalia Gladkova","doi":"10.1002/jbio.202300458","DOIUrl":"10.1002/jbio.202300458","url":null,"abstract":"<p>Detection of radiation-induced changes of the brain white matter is important for brain neoplasms repeated surgery. We investigated the influence of irradiation on the scattering properties of the white matter using optical coherence tomography (OCT). Healthy Wistar rats undergone the irradiation of the brain right hemisphere. At seven time points from the irradiation procedure (2–14 weeks), an ex vivo OCT study was performed with subsequent calculation of attenuation coefficient values in the corpus callosum followed by immunohistochemical analysis. As a result, we discovered acute and early-delayed changes characterized by the edema of different severity, accompanied by a statistically significant decrease in attenuation coefficient values. In particular, these changes were found at 2 weeks after irradiation in the irradiated hemisphere, while at 6- and 12-week time points they affected both irradiated and contralateral hemisphere. Thus, radiation-induced changes occurring in white matter during the first 3 months after irradiation can be detected by OCT.</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139522438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aline Knab, Ayad G. Anwer, Bernadette Pedersen, Shannon Handley, Abhilash Goud Marupally, Abbas Habibalahi, Ewa M. Goldys
This study focuses on the use of cellular autofluorescence which visualizes the cell metabolism by monitoring endogenous fluorophores including NAD(P)H and flavins. It explores the potential of multispectral imaging of native fluorophores in melanoma diagnostics using excitation wavelengths ranging from 340 nm to 510 nm and emission wavelengths above 391 nm. Cultured immortalized cells are utilized to compare the autofluorescent signatures of two melanoma cell lines to one fibroblast cell line. Feature analysis identifies the most significant and least correlated features for differentiating the cells. The investigation successfully applies this analysis to pre-processed, noise-removed images and original background-corrupted data. Furthermore, the applicability of distinguishing melanomas and healthy fibroblasts based on their autofluorescent characteristics is validated using the same evaluation technique on patient cells. Additionally, the study tentatively maps the detected features to underlying biological processes. This research demonstrates the potential of cellular autofluorescence as a promising tool for melanoma diagnostics.
{"title":"Towards label-free non-invasive autofluorescence multispectral imaging for melanoma diagnosis","authors":"Aline Knab, Ayad G. Anwer, Bernadette Pedersen, Shannon Handley, Abhilash Goud Marupally, Abbas Habibalahi, Ewa M. Goldys","doi":"10.1002/jbio.202300402","DOIUrl":"10.1002/jbio.202300402","url":null,"abstract":"<p>This study focuses on the use of cellular autofluorescence which visualizes the cell metabolism by monitoring endogenous fluorophores including NAD(P)H and flavins. It explores the potential of multispectral imaging of native fluorophores in melanoma diagnostics using excitation wavelengths ranging from 340 nm to 510 nm and emission wavelengths above 391 nm. Cultured immortalized cells are utilized to compare the autofluorescent signatures of two melanoma cell lines to one fibroblast cell line. Feature analysis identifies the most significant and least correlated features for differentiating the cells. The investigation successfully applies this analysis to pre-processed, noise-removed images and original background-corrupted data. Furthermore, the applicability of distinguishing melanomas and healthy fibroblasts based on their autofluorescent characteristics is validated using the same evaluation technique on patient cells. Additionally, the study tentatively maps the detected features to underlying biological processes. This research demonstrates the potential of cellular autofluorescence as a promising tool for melanoma diagnostics.</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbio.202300402","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139514355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In acute intestinal ischemia, the progression of ischemia varies across different layers of intestinal tissue. We established a mouse model and used swept-source optical coherence tomography (OCT) to observe the intestinal ischemic process longitudinally in different tissue layers. Employing a method that combines asymmetric gradient filtering with adaptive weighting, we eliminated the vessel trailing phenomenon in OCT angiograms, reducing the confounding effects of superficial vessels on the imaging of deeper vasculature. We quantitatively assessed changes in vascular perfusion density (VPD), vessel length, and vessel average diameter across various intestinal layers. Our results showed a significant reduction in VPD in all layers during ischemia. The mucosa layer experienced the most significant impact, primarily due to disrupted capillary blood flow, followed by the submucosa layer, where vascular constriction or decreased velocity was the primary factor.
在急性肠缺血中,肠组织不同层的缺血进展各不相同。我们建立了一个小鼠模型,并使用扫源光学相干断层扫描(OCT)技术纵向观察不同组织层的肠缺血过程。我们采用非对称梯度滤波与自适应加权相结合的方法,消除了 OCT 血管图中的血管拖尾现象,减少了浅层血管对深层血管成像的干扰。我们定量评估了肠道各层血管灌注密度(VPD)、血管长度和血管平均直径的变化。结果显示,缺血期间各层的血管灌注密度都明显下降。粘膜层受到的影响最大,这主要是由于毛细血管血流受到破坏,其次是粘膜下层,血管收缩或流速降低是主要因素。
{"title":"Study of ischemic progression in different intestinal tissue layers during acute intestinal ischemia using swept-source optical coherence tomography angiography","authors":"Yu Tian, Mingshuo Zhang, Hongbo Man, Chunnan Wu, Yimin Wang, Linghui Kong, Jian Liu","doi":"10.1002/jbio.202300382","DOIUrl":"10.1002/jbio.202300382","url":null,"abstract":"<p>In acute intestinal ischemia, the progression of ischemia varies across different layers of intestinal tissue. We established a mouse model and used swept-source optical coherence tomography (OCT) to observe the intestinal ischemic process longitudinally in different tissue layers. Employing a method that combines asymmetric gradient filtering with adaptive weighting, we eliminated the vessel trailing phenomenon in OCT angiograms, reducing the confounding effects of superficial vessels on the imaging of deeper vasculature. We quantitatively assessed changes in vascular perfusion density (VPD), vessel length, and vessel average diameter across various intestinal layers. Our results showed a significant reduction in VPD in all layers during ischemia. The mucosa layer experienced the most significant impact, primarily due to disrupted capillary blood flow, followed by the submucosa layer, where vascular constriction or decreased velocity was the primary factor.</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139514354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The only existing approach for assessing the risk of developing acute ischemic stroke (AIS) necessitates that individuals possess a strong understanding of their health status. Our research gathered compelling evidence in favor of our hypothesis, suggesting that the likelihood of developing AIS can be assessed by analyzing the green autofluorescence (AF) of the skin and fingernails. Utilizing machine learning-based analyses of AF images, we found that the area under the curve (AUC) for distinguishing subjects with three risk factors from those with zero, one, or two risk factors was 0.79, 0.76, and 0.75, respectively. Our research has revealed that green AF serves as an innovative biomarker for assessing the risk of developing AIS. Our method is objective, non-invasive, efficient, and economic, which shows great promise to boost a technology for screening natural populations for risk of developing AIS.
现有的唯一一种评估急性缺血性中风(AIS)发病风险的方法要求个人对自己的健康状况有充分的了解。我们的研究收集了支持我们假设的有力证据,表明可以通过分析皮肤和指甲的绿色自动荧光(AF)来评估罹患急性缺血性中风的可能性。通过对 AF 图像进行基于机器学习的分析,我们发现将具有三个风险因素的受试者与具有零、一个或两个风险因素的受试者区分开来的曲线下面积(AUC)分别为 0.79、0.76 和 0.75。我们的研究表明,绿色心房颤动可作为评估罹患 AIS 风险的创新生物标志物。我们的方法客观、无创、高效、经济,有望推动自然人群罹患 AIS 风险筛查技术的发展。
{"title":"Green autofluorescence of the skin and fingernails is a novel biomarker for evaluating the risk for developing acute ischemic stroke","authors":"Yue Tao, Haibo Yu, Mingchao Zhang, Xiaofeng Zou, Peilu Li, Jian-Ge Qiu, Bing-Hua Jiang, Weihai Ying","doi":"10.1002/jbio.202300473","DOIUrl":"10.1002/jbio.202300473","url":null,"abstract":"<p>The only existing approach for assessing the risk of developing acute ischemic stroke (AIS) necessitates that individuals possess a strong understanding of their health status. Our research gathered compelling evidence in favor of our hypothesis, suggesting that the likelihood of developing AIS can be assessed by analyzing the green autofluorescence (AF) of the skin and fingernails. Utilizing machine learning-based analyses of AF images, we found that the area under the curve (AUC) for distinguishing subjects with three risk factors from those with zero, one, or two risk factors was 0.79, 0.76, and 0.75, respectively. Our research has revealed that green AF serves as an innovative biomarker for assessing the risk of developing AIS. Our method is objective, non-invasive, efficient, and economic, which shows great promise to boost a technology for screening natural populations for risk of developing AIS.</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139514352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Merve Turker-Burhan, Ender Berat Ellidokuz, Husnu Alper Bagriyanik, Serhat Tozburun
This article investigates an endoscopic approach that utilizes negative pressure to achieve laser-induced thermal coagulation limited to the esophageal wall's mucosal and superficial submucosal layers. The study was built upon a series of studies combining numerical simulation based on the Monte-Carlo technique and ex vivo porcine tissue experiments, including apparatus design and histology analysis. An endoscopy apparatus was developed using 3D printing to validate the tissue stretching-based approach. A fiber-pigtailed diode was used as the near-infrared source, emitting 208.8 W/cm2 laser irradiance at 1.5 μm. Simulation results suggested that the approach successfully created a local heat well to prevent residual thermal effects (>65°C) from penetrating the deeper submucosal layer. Histology analysis of ex vivo tissues showed that at a fluence of 5.22 kJ/cm2, the depth of thermal coagulation was reduced by half compared to the control. With further preclinical studies, including endoscopy apparatus design, the approach can be applied to the larger esophageal surface.
{"title":"An endoscopic approach providing near-infrared laser-induced coagulation with accurate depth limits","authors":"Merve Turker-Burhan, Ender Berat Ellidokuz, Husnu Alper Bagriyanik, Serhat Tozburun","doi":"10.1002/jbio.202300377","DOIUrl":"10.1002/jbio.202300377","url":null,"abstract":"<p>This article investigates an endoscopic approach that utilizes negative pressure to achieve laser-induced thermal coagulation limited to the esophageal wall's mucosal and superficial submucosal layers. The study was built upon a series of studies combining numerical simulation based on the Monte-Carlo technique and ex vivo porcine tissue experiments, including apparatus design and histology analysis. An endoscopy apparatus was developed using 3D printing to validate the tissue stretching-based approach. A fiber-pigtailed diode was used as the near-infrared source, emitting 208.8 W/cm<sup>2</sup> laser irradiance at 1.5 μm. Simulation results suggested that the approach successfully created a local heat well to prevent residual thermal effects (>65°C) from penetrating the deeper submucosal layer. Histology analysis of ex vivo tissues showed that at a fluence of 5.22 kJ/cm<sup>2</sup>, the depth of thermal coagulation was reduced by half compared to the control. With further preclinical studies, including endoscopy apparatus design, the approach can be applied to the larger esophageal surface.</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbio.202300377","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139514350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Drosophila model has been widely used to study cardiac functions, especially combined with optogenetics and optical coherence tomography (OCT) that can continuously acquire mass cross-sectional images of the Drosophila heart in vivo over time. It's urgent to quickly and accurately obtain dynamic Drosophila cardiac parameters such as heartbeat rate for cardiac function quantitative analysis through these mass cross-sectional images of the Drosophila heart. Here we present a deep-learning method that integrates U-Net and generative adversarial network architectures while incorporating residually connected convolutions for high-precision OCT image segmentation of Drosophila heart and dynamic cardiac parameter measurements for optogenetics-OCT-based cardiac function research. We compared our proposed network with the previous approaches and our segmentation results achieved the accuracy of intersection over union and Dice similarity coefficient higher than 98%, which can be used to better quantify dynamic heart parameters and improve the efficiency of Drosophila-model-based cardiac research via the optogenetics-OCT-based platform.
果蝇模型已被广泛用于心脏功能的研究,尤其是结合光遗传学和光学相干断层扫描(OCT)技术,可在活体中长时间连续获取果蝇心脏的质量截面图像。通过这些果蝇心脏的质量横截面图像快速准确地获得动态的果蝇心脏参数(如心跳频率)以进行心脏功能定量分析已迫在眉睫。在此,我们提出了一种深度学习方法,该方法整合了 U-Net 和生成对抗网络架构,同时结合了残差连接卷积,用于果蝇心脏的高精度 OCT 图像分割和动态心脏参数测量,以开展基于光遗传学-OCT 的心脏功能研究。我们将所提出的网络与之前的方法进行了比较,我们的分割结果达到了交集大于联合的精度,Dice相似系数高于98%,可用于更好地量化动态心脏参数,并通过基于光遗传学-OCT平台提高基于果蝇模型的心脏研究效率。
{"title":"High-precision Drosophila heart segmentation and dynamic cardiac parameter measurement for optogenetics-OCT-based cardiac function research","authors":"Fei Zheng, Renxiong Wu, Shaoyan Huang, Meixuan Li, Wuzhou Yuan, Guangming Ni, Yong Liu","doi":"10.1002/jbio.202300447","DOIUrl":"10.1002/jbio.202300447","url":null,"abstract":"<p>Drosophila model has been widely used to study cardiac functions, especially combined with optogenetics and optical coherence tomography (OCT) that can continuously acquire mass cross-sectional images of the Drosophila heart in vivo over time. It's urgent to quickly and accurately obtain dynamic Drosophila cardiac parameters such as heartbeat rate for cardiac function quantitative analysis through these mass cross-sectional images of the Drosophila heart. Here we present a deep-learning method that integrates U-Net and generative adversarial network architectures while incorporating residually connected convolutions for high-precision OCT image segmentation of Drosophila heart and dynamic cardiac parameter measurements for optogenetics-OCT-based cardiac function research. We compared our proposed network with the previous approaches and our segmentation results achieved the accuracy of intersection over union and Dice similarity coefficient higher than 98%, which can be used to better quantify dynamic heart parameters and improve the efficiency of Drosophila-model-based cardiac research via the optogenetics-OCT-based platform.</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139492423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Priyanka A. Jadhav, Arti Hole, Arvind Ingle, Rukmini Govekar, Hemanth Noothalapati, C. Murali Krishna
Several serum Raman spectroscopy (RS) studies have demonstrated its potential as an oral cancer screening tool. This study investigates influence of low tumour load (LTL) and high tumour load (HTL) on serum RS using hamster buccal pouch model of experimental oral carcinogenesis. Sera of untreated control, LTL, and HTL groups at week intervals during malignant transformation were employed. Serum Raman spectra were subjected to multivariate analyses—principal component analysis, principal component-based linear discriminant analysis (for stratification of study groups), and multivariate curve resolution-alternating least squares (MCR-ALS) (to comprehend biomolecular differences). Multivariate analysis revealed misclassifications between LTL and HTL at all week intervals. MCR-ALS components showed statistically significant abundances between control versus LTL and control versus HTL, but could not discern LTL and HTL. MCR-ALS components exhibited spectral mixtures of proteins, lipids, heme and nucleic acids. Thus, these findings support use of serum RS as a screening tool as varying tumour load is not a confounding factor influencing the technique.
{"title":"Serum Raman spectroscopy: Evaluation of tumour load variations in experimental carcinogenesis","authors":"Priyanka A. Jadhav, Arti Hole, Arvind Ingle, Rukmini Govekar, Hemanth Noothalapati, C. Murali Krishna","doi":"10.1002/jbio.202300424","DOIUrl":"10.1002/jbio.202300424","url":null,"abstract":"<p>Several serum Raman spectroscopy (RS) studies have demonstrated its potential as an oral cancer screening tool. This study investigates influence of low tumour load (LTL) and high tumour load (HTL) on serum RS using hamster buccal pouch model of experimental oral carcinogenesis. Sera of untreated control, LTL, and HTL groups at week intervals during malignant transformation were employed. Serum Raman spectra were subjected to multivariate analyses—principal component analysis, principal component-based linear discriminant analysis (for stratification of study groups), and multivariate curve resolution-alternating least squares (MCR-ALS) (to comprehend biomolecular differences). Multivariate analysis revealed misclassifications between LTL and HTL at all week intervals. MCR-ALS components showed statistically significant abundances between control versus LTL and control versus HTL, but could not discern LTL and HTL. MCR-ALS components exhibited spectral mixtures of proteins, lipids, heme and nucleic acids. Thus, these findings support use of serum RS as a screening tool as varying tumour load is not a confounding factor influencing the technique.</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139479730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xiao Han, Yubao Zhang, Gang Shi, Guo Liu, Sizhu Ai, Yidi Wang, Qin Zhang, Xingdao He
Quantifying corneal elasticity after femtosecond laser-assisted in situ keratomileusis (FS-LASIK) procedure plays an important role in improving surgical safety and quality, since some latent complications may occur ascribing to changes in postoperative corneal biomechanics. Nevertheless, it is suggested that current research has been severely constrained due to the lack of an accurate quantification method to obtain postoperative corneal elasticity distribution. In this paper, an acoustic radiation force optical coherence elastography system combined with the improved phase velocity algorithm was utilized to realize elasticity distribution images of the in vivo rabbit cornea after FS-LASIK under various intraocular pressure levels. As a result, elasticity variations within and between the regions of interest could be identified precisely. This is the first time that elasticity imaging of in vivo cornea after FS-LASIK surgery was demonstrated, and the results suggested that this technology may hold promise in further exploring corneal biomechanical properties after refractive surgery.
{"title":"Quantitative assessment of corneal elasticity distribution after FS-LASIK using optical coherence elastography","authors":"Xiao Han, Yubao Zhang, Gang Shi, Guo Liu, Sizhu Ai, Yidi Wang, Qin Zhang, Xingdao He","doi":"10.1002/jbio.202300441","DOIUrl":"10.1002/jbio.202300441","url":null,"abstract":"<p>Quantifying corneal elasticity after femtosecond laser-assisted in situ keratomileusis (FS-LASIK) procedure plays an important role in improving surgical safety and quality, since some latent complications may occur ascribing to changes in postoperative corneal biomechanics. Nevertheless, it is suggested that current research has been severely constrained due to the lack of an accurate quantification method to obtain postoperative corneal elasticity distribution. In this paper, an acoustic radiation force optical coherence elastography system combined with the improved phase velocity algorithm was utilized to realize elasticity distribution images of the in vivo rabbit cornea after FS-LASIK under various intraocular pressure levels. As a result, elasticity variations within and between the regions of interest could be identified precisely. This is the first time that elasticity imaging of in vivo cornea after FS-LASIK surgery was demonstrated, and the results suggested that this technology may hold promise in further exploring corneal biomechanical properties after refractive surgery.</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139467588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xiaoqian Lin, Changfeng Yang, Yijie Lv, Bowen Zhang, Junnan Kan, Hao Li, Jin Tao, Caixia Yang, Xianglin Li, Yan Liu
Optoacoustic imaging enables the measurement of tissue oxygen saturation (sO2) and blood perfusion while being utilized for detecting tumor microenvironments. Our aim was to employ multispectral optoacoustic tomography (MSOT) to assess immediate-early changes of hemoglobin level and sO2 within breast tumors during diverse treatments. Mouse breast cancer models were allocated into four groups: control, everolimus (EVE), paclitaxel (PTX), and photodynamic therapy (PDT). Hemoglobin was quantified daily, as well as sO2 and blood perfusion were verified by immunohistochemical (IHC) staining. MSOT showed a temporal window of enhanced oxygenation and improved perfusion in EVE and PTX groups, while sO2 consistently remained below baseline in PDT. The same results were obtained for the IHC. Therefore, MSOT can monitor tumor hypoxia and indirectly reflect blood perfusion in a non-invasive and non-labeled way, which has the potential to monitor breast cancer progression early and enable individualized treatment in clinical practice.
{"title":"Preclinical multi-physiologic monitoring of immediate-early responses to diverse treatment strategies in breast cancer by optoacoustic imaging","authors":"Xiaoqian Lin, Changfeng Yang, Yijie Lv, Bowen Zhang, Junnan Kan, Hao Li, Jin Tao, Caixia Yang, Xianglin Li, Yan Liu","doi":"10.1002/jbio.202300457","DOIUrl":"10.1002/jbio.202300457","url":null,"abstract":"<p>Optoacoustic imaging enables the measurement of tissue oxygen saturation (sO<sub>2</sub>) and blood perfusion while being utilized for detecting tumor microenvironments. Our aim was to employ multispectral optoacoustic tomography (MSOT) to assess immediate-early changes of hemoglobin level and sO<sub>2</sub> within breast tumors during diverse treatments. Mouse breast cancer models were allocated into four groups: control, everolimus (EVE), paclitaxel (PTX), and photodynamic therapy (PDT). Hemoglobin was quantified daily, as well as sO<sub>2</sub> and blood perfusion were verified by immunohistochemical (IHC) staining. MSOT showed a temporal window of enhanced oxygenation and improved perfusion in EVE and PTX groups, while sO<sub>2</sub> consistently remained below baseline in PDT. The same results were obtained for the IHC. Therefore, MSOT can monitor tumor hypoxia and indirectly reflect blood perfusion in a non-invasive and non-labeled way, which has the potential to monitor breast cancer progression early and enable individualized treatment in clinical practice.</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139467586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pancreatic intraepithelial neoplasia (PanIN) is the most common precursor lesion that has the potential to progress to invasive pancreatic cancer, and early and rapid detection may offer patients a chance for treatment before the development of invasive carcinoma. Therefore, the identification of PanIN holds significant clinical importance. In this study, we first used multiphoton microscopy (MPM) combining two-photon excitation fluorescence and second-harmonic generation imaging to label-free detect PanIN and attempted to differentiate between normal pancreatic ducts and different grades of PanIN. Then, we also developed an automatic image processing strategy to extract eight morphological features of collagen fibers from MPM images to quantify the changes in collagen fibers surrounding the ducts. Experimental results demonstrate that the combination of MPM and quantitative information can accurately identify normal pancreatic ducts and different grades of PanIN. This study may contribute to the rapid diagnosis of pancreatic diseases and may lay the foundation for further clinical application of MPM.
{"title":"Label-free assessment of pathological changes in pancreatic intraepithelial neoplasia by biomedical multiphoton microscopy","authors":"Jikui Miao, Zheng Zhang, Xiong Zhang, Xingxin Huang, Shichao Zhang, Zhenlin Zhan, Jianxin Chen, Linying Chen, Lianhuang Li","doi":"10.1002/jbio.202300417","DOIUrl":"10.1002/jbio.202300417","url":null,"abstract":"<p>Pancreatic intraepithelial neoplasia (PanIN) is the most common precursor lesion that has the potential to progress to invasive pancreatic cancer, and early and rapid detection may offer patients a chance for treatment before the development of invasive carcinoma. Therefore, the identification of PanIN holds significant clinical importance. In this study, we first used multiphoton microscopy (MPM) combining two-photon excitation fluorescence and second-harmonic generation imaging to label-free detect PanIN and attempted to differentiate between normal pancreatic ducts and different grades of PanIN. Then, we also developed an automatic image processing strategy to extract eight morphological features of collagen fibers from MPM images to quantify the changes in collagen fibers surrounding the ducts. Experimental results demonstrate that the combination of MPM and quantitative information can accurately identify normal pancreatic ducts and different grades of PanIN. This study may contribute to the rapid diagnosis of pancreatic diseases and may lay the foundation for further clinical application of MPM.</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139467583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}