Pub Date : 2017-04-02DOI: 10.26510/2394-0859.PBE.2017.17
Dr. P. Solairaj, .. Jebaseelan
Objective: The present work was executed to evaluate the anti-diabetic potency of a polyherbal preparation. The objective of this study is to induce experimental diabetes mellitus using streptozotocin in normal Albino wistar rats and study the antdiabetic activity of polyherbal formulation by comparison of changes in levels of glucose between normal and diabetic rats . Methods: The effect of methanol extract of poly herbal preparation containing aerial parts of Schrebera swietenoides, roots of Barleria montana and aerial parts of Rotula aquatica was investigated in normal and streptozotocin induced diabetic rats. Results: The lowest blood glucose levels were observed at 4 and 8 th hr after the oral administration of 150 and 300 mg/kg b.w polyherbal formulation. The blood glucose levels at 24hrs after the oral administration of 150 and 300 mg/kg b. w of poly herbal formulation was significantly lowers the blood glucose levels. Conclusions: The display of synergy or antagonism by the composite herbal extracts in ameliorating hyperglycemia depended on the type and number of individual herbal extract used in constituting the experimental herbal formulations.
目的:评价一种复方中药制剂的抗糖尿病作用。本研究采用链脲佐菌素诱导正常白化wistar大鼠实验性糖尿病，并通过比较正常大鼠和糖尿病大鼠葡萄糖水平的变化，研究复方的抗糖尿病活性。方法:采用链脲佐菌素诱导的糖尿病大鼠和正常大鼠实验，考察含甜菊属、大黄菜根和水轮藤属空中部位的复方中药制剂甲醇提取物对糖尿病大鼠的影响。结果:口服复方150、300 mg/kg b.w后4、8小时血糖最低。口服复方150、300 mg/kg b. w后24h血糖水平显著降低。结论:复方中草药提取物对高血糖的增效或拮抗作用取决于组成实验中草药配方的单个中草药提取物的种类和数量。
{"title":"Preparation and evaluation of polyherbal formulation for its antidiabetic activity against streptozotocin induced diabetes rat model","authors":"Dr. P. Solairaj, .. Jebaseelan","doi":"10.26510/2394-0859.PBE.2017.17","DOIUrl":"https://doi.org/10.26510/2394-0859.PBE.2017.17","url":null,"abstract":"Objective: The present work was executed to evaluate the anti-diabetic potency of a polyherbal preparation. The objective of this study is to induce experimental diabetes mellitus using streptozotocin in normal Albino wistar rats and study the antdiabetic activity of polyherbal formulation by comparison of changes in levels of glucose between normal and diabetic rats . Methods: The effect of methanol extract of poly herbal preparation containing aerial parts of Schrebera swietenoides, roots of Barleria montana and aerial parts of Rotula aquatica was investigated in normal and streptozotocin induced diabetic rats. Results: The lowest blood glucose levels were observed at 4 and 8 th hr after the oral administration of 150 and 300 mg/kg b.w polyherbal formulation. The blood glucose levels at 24hrs after the oral administration of 150 and 300 mg/kg b. w of poly herbal formulation was significantly lowers the blood glucose levels. Conclusions: The display of synergy or antagonism by the composite herbal extracts in ameliorating hyperglycemia depended on the type and number of individual herbal extract used in constituting the experimental herbal formulations.","PeriodicalId":19998,"journal":{"name":"Pharmaceutical and Biological Evaluations","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88557766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-04-02DOI: 10.26510/2394-0859.PBE.2017.13
S. Ganguly, Shivchand Yadav
There are various mycotic diseases of veterinary importance affecting the domestic animals. The diagnosis of the causative fungi or yeast responsible for those infections is essential in veterinary microbiological laboratories for confirmative diagnosis followed by its treatment and control.
{"title":"Mycoses in animals: fungal characteristics and prevention strategies","authors":"S. Ganguly, Shivchand Yadav","doi":"10.26510/2394-0859.PBE.2017.13","DOIUrl":"https://doi.org/10.26510/2394-0859.PBE.2017.13","url":null,"abstract":"There are various mycotic diseases of veterinary importance affecting the domestic animals. The diagnosis of the causative fungi or yeast responsible for those infections is essential in veterinary microbiological laboratories for confirmative diagnosis followed by its treatment and control.","PeriodicalId":19998,"journal":{"name":"Pharmaceutical and Biological Evaluations","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81031231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-04-02DOI: 10.26510/2394-0859.PBE.2017.19
Radhika Chelamalla
Molecularly targeted agents light at end of tunnel to resolve therapeutics by drawing together morbidity and mortality in patients by the whole of cancer. However, still an urgent has a passion for preferably effective anticancer compounds, state-of-the-art preclinical abused substance evaluations largely overlook to answer a need the demand. New preclinical strategies, including the review of with all the extras mouse models and co-clinical design designs, are for used to boost the predictive price tag of animal-based translational aries research. Here, we saw in a new light the society of born with a silver spoon preclinical antineoplastic agents, their associated limitations, and arbitrary methods to feel in one bones clinical outcomes.
{"title":"Drug resistance: important criteria for cancer drug development","authors":"Radhika Chelamalla","doi":"10.26510/2394-0859.PBE.2017.19","DOIUrl":"https://doi.org/10.26510/2394-0859.PBE.2017.19","url":null,"abstract":"Molecularly targeted agents light at end of tunnel to resolve therapeutics by drawing together morbidity and mortality in patients by the whole of cancer. However, still an urgent has a passion for preferably effective anticancer compounds, state-of-the-art preclinical abused substance evaluations largely overlook to answer a need the demand. New preclinical strategies, including the review of with all the extras mouse models and co-clinical design designs, are for used to boost the predictive price tag of animal-based translational aries research. Here, we saw in a new light the society of born with a silver spoon preclinical antineoplastic agents, their associated limitations, and arbitrary methods to feel in one bones clinical outcomes.","PeriodicalId":19998,"journal":{"name":"Pharmaceutical and Biological Evaluations","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82331805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-04-02DOI: 10.26510/2394-0859.PBE.2017.12
Mital B. Kansara, A. Jani
Herbs can affect body function; therefore, when herbs are taken concurrently with drugs, interactions are possible. The interaction between drugs and herbal medicines is a safe concern and these interactions are especially important for drugs with narrow therapeutics index. The probability of herb-drug interaction can be higher than drug interaction, if conventional drug having single chemical entities, whereas most of the herbal medicinal product contain a mixture of pharmacologically active constituents. The herb-drug interaction may involve either an increase or decrease in the amount of drug in blood, either by altering the absorption, distribution, metabolism, and elimination (ADME) of drug and by antagonizing or synergism of the effect of drug or pharmacodynamics interaction may arise. This article we focus on how garlic interacts with conventional drug or it is favourable or not.
{"title":"Possible interactions between garlic and conventional drugs: a review","authors":"Mital B. Kansara, A. Jani","doi":"10.26510/2394-0859.PBE.2017.12","DOIUrl":"https://doi.org/10.26510/2394-0859.PBE.2017.12","url":null,"abstract":"Herbs can affect body function; therefore, when herbs are taken concurrently with drugs, interactions are possible. The interaction between drugs and herbal medicines is a safe concern and these interactions are especially important for drugs with narrow therapeutics index. The probability of herb-drug interaction can be higher than drug interaction, if conventional drug having single chemical entities, whereas most of the herbal medicinal product contain a mixture of pharmacologically active constituents. The herb-drug interaction may involve either an increase or decrease in the amount of drug in blood, either by altering the absorption, distribution, metabolism, and elimination (ADME) of drug and by antagonizing or synergism of the effect of drug or pharmacodynamics interaction may arise. This article we focus on how garlic interacts with conventional drug or it is favourable or not.","PeriodicalId":19998,"journal":{"name":"Pharmaceutical and Biological Evaluations","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91312858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-04-02DOI: 10.26510/2394-0859.PBE.2017.11
D. S., Bhuvana B., H. S, J. C., Sathesh Kumar K
Generally seaweeds are an essential source in the field of pharmaceutical industry because of their bioactive metabolites. Life threatening diseases like acquired immuno defieciency syndrome (AIDS), cancer and other infectious diseases can be trated with the constituents present in the Gracilaria species. This paper deals with a compilation of the literature for Gracilaria corticata algae extracts from the beginning of this century with its pharmacological benefits and recognizes opportunities for forthcoming research.
{"title":"Therapeutic potential and pharmacological significance of the marine algae Gracilaria corticata","authors":"D. S., Bhuvana B., H. S, J. C., Sathesh Kumar K","doi":"10.26510/2394-0859.PBE.2017.11","DOIUrl":"https://doi.org/10.26510/2394-0859.PBE.2017.11","url":null,"abstract":"Generally seaweeds are an essential source in the field of pharmaceutical industry because of their bioactive metabolites. Life threatening diseases like acquired immuno defieciency syndrome (AIDS), cancer and other infectious diseases can be trated with the constituents present in the Gracilaria species. This paper deals with a compilation of the literature for Gracilaria corticata algae extracts from the beginning of this century with its pharmacological benefits and recognizes opportunities for forthcoming research.","PeriodicalId":19998,"journal":{"name":"Pharmaceutical and Biological Evaluations","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87097039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-04-02DOI: 10.26510/2394-0859.PBE.2017.16
S. Chowdhury, M. Babu, K. Ankitha, B. Shirisha, Madhurika Sirigadi, E. Kavya
Objective: The Present investigation was performed to find out the effect of synthetic and natural polymers on the release properties of glimepiride matrix tablet. Glimepiride is a first third generation sulphonyl urea agent for the treatment of type- II diabetes mellitus. Methocel K15M, Olibanum Gum were used as key release modifying polymers. Methods: Nine formulations were prepared taking different concentration of natural and synthetic polymers, The drug excipient mixtures were subjected to pre-compression studies. The tablets were prepared by direct compression method; all formulations were subjected to physicochemical studies, in- vitro drug release, kinetic studies and stability studies. The physicochemical results were found within the limits. Results: FTIR study interpretation did not show any drug–excipient interaction The drug release from the optimized formulation F-7 was extended for a period of 12 hours. The release kinetics of F-7 formulation showed that the release of drug follows zero order models. The optimized formulations were subjected to stability studies and shown there were no significant changes in drug content, physicochemical parameters and release pattern. Conclusions: Results of the present study indicated the suitability of the above mentioned polymers in the preparation of sustained release formulation of Glimepiride for the management of type-II diabetes mellitus effectively.
{"title":"A comparative study on effect of polymers on release kinetics glimepiride matrix tablet","authors":"S. Chowdhury, M. Babu, K. Ankitha, B. Shirisha, Madhurika Sirigadi, E. Kavya","doi":"10.26510/2394-0859.PBE.2017.16","DOIUrl":"https://doi.org/10.26510/2394-0859.PBE.2017.16","url":null,"abstract":"Objective: The Present investigation was performed to find out the effect of synthetic and natural polymers on the release properties of glimepiride matrix tablet. Glimepiride is a first third generation sulphonyl urea agent for the treatment of type- II diabetes mellitus. Methocel K15M, Olibanum Gum were used as key release modifying polymers. Methods: Nine formulations were prepared taking different concentration of natural and synthetic polymers, The drug excipient mixtures were subjected to pre-compression studies. The tablets were prepared by direct compression method; all formulations were subjected to physicochemical studies, in- vitro drug release, kinetic studies and stability studies. The physicochemical results were found within the limits. Results: FTIR study interpretation did not show any drug–excipient interaction The drug release from the optimized formulation F-7 was extended for a period of 12 hours. The release kinetics of F-7 formulation showed that the release of drug follows zero order models. The optimized formulations were subjected to stability studies and shown there were no significant changes in drug content, physicochemical parameters and release pattern. Conclusions: Results of the present study indicated the suitability of the above mentioned polymers in the preparation of sustained release formulation of Glimepiride for the management of type-II diabetes mellitus effectively.","PeriodicalId":19998,"journal":{"name":"Pharmaceutical and Biological Evaluations","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80375768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-02-07DOI: 10.26510/2394-0859.PBE.2017.09
S. Tanniru, S. Asha, Lavanya Poonem, Divya Murahari, M. Jojula, T. Šuman
Objective : Tuberculosis is one of the important infections among the diabetes as per our study and survey reports. Diabetes mellitus has been reported to modify the presenting features of pulmonary tuberculosis, but there are varying data, particularly regarding the association with lower lung field involvement. In our study we would like to determine whether diabetes mellitus alters the clinical and radiographic manifestations of tuberculosis in hosts and to define the determinants of lower lung field involvement due to effect of nutrition supply. Methods : A total of 500 diabetes were identified from both rural and urban population and were confirmed the diabetic status based on questioner and consent of patients. Off 500 patients 200 suspects with bronchial infections was tested for other bacterial and Tuberculosis infections by Grams staining, and culture on Nutrient and blood Agar for bacterial infections based on the staining and culture they were identified to be grams positive or Grams negative bacterial infections. Results: We had conducted a survey and study in Warangal and adjoing districts of Telangana state during 2014- 2017 period, in the study we had counseled approximately 1000 -2000 populations off which 500 were suspects for early diabetes based on the symptoms and clinical investigations off 500 were able to found 200 diabetic type 2 causes. Off 200 55 were positive for tuberculosis all the suspects were counseled for their nutritional status and other prevailing side effects with treatment . Conclusions: Based on this study we had tried to evaluate the effect, care and support of nutritional among diabetic TB patients.
{"title":"Nutritional effect, care and support for patients with tuberculosis among diabetes: a study from Warangal district","authors":"S. Tanniru, S. Asha, Lavanya Poonem, Divya Murahari, M. Jojula, T. Šuman","doi":"10.26510/2394-0859.PBE.2017.09","DOIUrl":"https://doi.org/10.26510/2394-0859.PBE.2017.09","url":null,"abstract":"Objective : Tuberculosis is one of the important infections among the diabetes as per our study and survey reports. Diabetes mellitus has been reported to modify the presenting features of pulmonary tuberculosis, but there are varying data, particularly regarding the association with lower lung field involvement. In our study we would like to determine whether diabetes mellitus alters the clinical and radiographic manifestations of tuberculosis in hosts and to define the determinants of lower lung field involvement due to effect of nutrition supply. Methods : A total of 500 diabetes were identified from both rural and urban population and were confirmed the diabetic status based on questioner and consent of patients. Off 500 patients 200 suspects with bronchial infections was tested for other bacterial and Tuberculosis infections by Grams staining, and culture on Nutrient and blood Agar for bacterial infections based on the staining and culture they were identified to be grams positive or Grams negative bacterial infections. Results: We had conducted a survey and study in Warangal and adjoing districts of Telangana state during 2014- 2017 period, in the study we had counseled approximately 1000 -2000 populations off which 500 were suspects for early diabetes based on the symptoms and clinical investigations off 500 were able to found 200 diabetic type 2 causes. Off 200 55 were positive for tuberculosis all the suspects were counseled for their nutritional status and other prevailing side effects with treatment . Conclusions: Based on this study we had tried to evaluate the effect, care and support of nutritional among diabetic TB patients.","PeriodicalId":19998,"journal":{"name":"Pharmaceutical and Biological Evaluations","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75169177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-02-07DOI: 10.26510/2394-0859.pbe.2017.08
V. Vijayalakshmi, S. Krishnaveni, G. Madhuramozhi
Objective s: To explore the acute toxicity of major aquatic pollutants like mercuric chloride (HgCl 2 ) and polychlorinated by phenyls (PCB) and their effects based on the haematology of fresh water fish Danio rerio (Zebra fish). Methods : Zebra fish ( Danio rerio ) were exposed to progressive concentrations of HgCl 2 and PCB. The fishes were exposed to various concentrations of 5, 10, 20, 30, 40, 50 and 60 µg/L of mercury and PCB for a period up to two weeks. Haematological tests (total RBC, WBC count and haemoglobin concentration) were carried out on each concentration as well as control for a period of 2 weeks. Results : It was observed that, in exposure time from 0, 7 and 14 days, the total erythrocyte (RBC), leucocytes (WBC) haemoglobin (Hb) values decreased. The exposure to sub lethal doses of Mercury and PCB for 1, 7 and 14 days exposure period causes significant(Values are expressed as means ± SD. Significant difference between groups were compared to control, *p <0.01, **p <0.05) time and dose dependent alterations in total RBC, WBC and haemoglobin count compared to the control values. Conclusions : Haematological parameters constitute one of the important methods to access the health or ill health of an organism, subjected to intoxication. This demonstrates the physiological dysfunction of the haemopoietic system. Hgcl 2 is a heavy metal that causes symptoms in human like and gastrointestinal disturbances, anaemia, anorexia, loss of weight, chronic inflammations of kidneys. PCB exposure in animals has been reported to develop Wasting syndrome, reduced body weight, immune toxicity, vitamin A deficiency, and thyroid hormone deficiency, reproductive effects in offspring like reduced birth weight, abnormal gonad development. It also affect the central nervous system which results in slowed learning and memory loss, and also responsible for other behavioural changes. Thus toxicity bioassay is the basic tool for the detection, evaluation and abatement of water pollution
{"title":"Toxic manifestations of mercuric chloride and polychlorinated biphenyls on the haematological aspects of Danio rerio","authors":"V. Vijayalakshmi, S. Krishnaveni, G. Madhuramozhi","doi":"10.26510/2394-0859.pbe.2017.08","DOIUrl":"https://doi.org/10.26510/2394-0859.pbe.2017.08","url":null,"abstract":"Objective s: To explore the acute toxicity of major aquatic pollutants like mercuric chloride (HgCl 2 ) and polychlorinated by phenyls (PCB) and their effects based on the haematology of fresh water fish Danio rerio (Zebra fish). Methods : Zebra fish ( Danio rerio ) were exposed to progressive concentrations of HgCl 2 and PCB. The fishes were exposed to various concentrations of 5, 10, 20, 30, 40, 50 and 60 µg/L of mercury and PCB for a period up to two weeks. Haematological tests (total RBC, WBC count and haemoglobin concentration) were carried out on each concentration as well as control for a period of 2 weeks. Results : It was observed that, in exposure time from 0, 7 and 14 days, the total erythrocyte (RBC), leucocytes (WBC) haemoglobin (Hb) values decreased. The exposure to sub lethal doses of Mercury and PCB for 1, 7 and 14 days exposure period causes significant(Values are expressed as means ± SD. Significant difference between groups were compared to control, *p <0.01, **p <0.05) time and dose dependent alterations in total RBC, WBC and haemoglobin count compared to the control values. Conclusions : Haematological parameters constitute one of the important methods to access the health or ill health of an organism, subjected to intoxication. This demonstrates the physiological dysfunction of the haemopoietic system. Hgcl 2 is a heavy metal that causes symptoms in human like and gastrointestinal disturbances, anaemia, anorexia, loss of weight, chronic inflammations of kidneys. PCB exposure in animals has been reported to develop Wasting syndrome, reduced body weight, immune toxicity, vitamin A deficiency, and thyroid hormone deficiency, reproductive effects in offspring like reduced birth weight, abnormal gonad development. It also affect the central nervous system which results in slowed learning and memory loss, and also responsible for other behavioural changes. Thus toxicity bioassay is the basic tool for the detection, evaluation and abatement of water pollution","PeriodicalId":19998,"journal":{"name":"Pharmaceutical and Biological Evaluations","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82752549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-02-07DOI: 10.26510/2394-0859.PBE.2017.05
P. Rajeswari, P. Samuel, J. Vijayakumar, T. Selvarathinam, Sudarmani D.N.P., K. Amirtharaj, R. Deenathayalan
Objective : The objective of the present study is to produce silver nanoparticles from marine fungi from south west coastal areas of Tamil Nadu and evaluating their potentials with special reference to anticancer activity. Methods : Hand core pushing technique is adapted to collect marine sediment samples along the coastal environs. Distinguished Aspergillus colonies were isolated and identified by wet mount procedure. The characterized Aspergillus consortium was subjected to produce silver nanoparticles. The extracellularly synthesized nanoparticles were characterized. Silver nanoparticles were evaluated for anticancer activity against MCF7 cell line by MTT assay. The IC 50 values were determined. Results : Aspergillus consortium consist of A. niger, A. michelle and A. japonicus . Silver nanoparticles were extracellularly synthesized by the reduction of silver nitrate (AgNO 3 ) to metallic silver (Ag + ) ions results in the transformation of pale yellow to dark red colour. Constant shift was observed at 420 nm while monitoring the solution by UV-Vis spectrophotometer. The presence of important functional groups like –NH 3 was confirmed by FT-IR Spectroscopy. Anticancer activity of the silver nanoparticles was evaluated against MCF7. There was 100% cell inhibition when concentration of the AgNPs reached 25 µg, 50 µg and 100 µg respectively in the test solution. Notably the IC 50 value was found to be very lowest for the nanoparticles produced by A. japonicus and the value was found to be 1.47 µg/ml. Conclusions : Aspergillus consortium was found to be an ideal mycobiosystem for the production of silver nanoparticles with potential anticancer activity.
{"title":"Green synthesis of silver nanoparticles by Aspergillus consortium and evaluating its anticancer activity against Human breast adenocarcinoma cell line (MCF7)","authors":"P. Rajeswari, P. Samuel, J. Vijayakumar, T. Selvarathinam, Sudarmani D.N.P., K. Amirtharaj, R. Deenathayalan","doi":"10.26510/2394-0859.PBE.2017.05","DOIUrl":"https://doi.org/10.26510/2394-0859.PBE.2017.05","url":null,"abstract":"Objective : The objective of the present study is to produce silver nanoparticles from marine fungi from south west coastal areas of Tamil Nadu and evaluating their potentials with special reference to anticancer activity. Methods : Hand core pushing technique is adapted to collect marine sediment samples along the coastal environs. Distinguished Aspergillus colonies were isolated and identified by wet mount procedure. The characterized Aspergillus consortium was subjected to produce silver nanoparticles. The extracellularly synthesized nanoparticles were characterized. Silver nanoparticles were evaluated for anticancer activity against MCF7 cell line by MTT assay. The IC 50 values were determined. Results : Aspergillus consortium consist of A. niger, A. michelle and A. japonicus . Silver nanoparticles were extracellularly synthesized by the reduction of silver nitrate (AgNO 3 ) to metallic silver (Ag + ) ions results in the transformation of pale yellow to dark red colour. Constant shift was observed at 420 nm while monitoring the solution by UV-Vis spectrophotometer. The presence of important functional groups like –NH 3 was confirmed by FT-IR Spectroscopy. Anticancer activity of the silver nanoparticles was evaluated against MCF7. There was 100% cell inhibition when concentration of the AgNPs reached 25 µg, 50 µg and 100 µg respectively in the test solution. Notably the IC 50 value was found to be very lowest for the nanoparticles produced by A. japonicus and the value was found to be 1.47 µg/ml. Conclusions : Aspergillus consortium was found to be an ideal mycobiosystem for the production of silver nanoparticles with potential anticancer activity.","PeriodicalId":19998,"journal":{"name":"Pharmaceutical and Biological Evaluations","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87635932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-02-07DOI: 10.26510/2394-0859.PBE.2017.03
H. Jain, Kinjal D. Patel, N. Savant, U. Upadhyay
Objective : The aim of the present study was to formulate the floating bilayer tablets of Ketorolac tromethamine, first immediate release layer and second sustained release floating layer which would provide initial loading dose of drug and remain in stomach and upper part of GIT for prolonged period of time in a view to maximize solubility of drug which is necessary for its absorption. Methods : The floating bilayered tablets of Ketorolac tromethamine were prepared by using 3 2 factorial designs by direct compression method. For this, polymers like sodium starch glycolate and polyox WSR 303 were used in various concentrations. Sodium bicarbonate was used as a floating effervescent agent. The formulations were evaluated for hardness, friability, weight variation, swelling index, floating lag time, floating time, % CDR etc Results : From the result obtained, S3 having 23 % Polyox WSR 303 and 12 % sodium bicarbonate showed better results. Conclusions : The results showed that Polyox WSR is promising tool to designing of sustained release formulation.
{"title":"Optimization of floating bilayered tablets of Ketorolac Tromethamine","authors":"H. Jain, Kinjal D. Patel, N. Savant, U. Upadhyay","doi":"10.26510/2394-0859.PBE.2017.03","DOIUrl":"https://doi.org/10.26510/2394-0859.PBE.2017.03","url":null,"abstract":"Objective : The aim of the present study was to formulate the floating bilayer tablets of Ketorolac tromethamine, first immediate release layer and second sustained release floating layer which would provide initial loading dose of drug and remain in stomach and upper part of GIT for prolonged period of time in a view to maximize solubility of drug which is necessary for its absorption. Methods : The floating bilayered tablets of Ketorolac tromethamine were prepared by using 3 2 factorial designs by direct compression method. For this, polymers like sodium starch glycolate and polyox WSR 303 were used in various concentrations. Sodium bicarbonate was used as a floating effervescent agent. The formulations were evaluated for hardness, friability, weight variation, swelling index, floating lag time, floating time, % CDR etc Results : From the result obtained, S3 having 23 % Polyox WSR 303 and 12 % sodium bicarbonate showed better results. Conclusions : The results showed that Polyox WSR is promising tool to designing of sustained release formulation.","PeriodicalId":19998,"journal":{"name":"Pharmaceutical and Biological Evaluations","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77681928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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