Daniel Alfandari, Irit Rosenhek-Goldian, Ewa Kozela, Reinat Nevo, Marcela Bahlsen Senprún, Anton Moisieiev, Noam Sogauker, Ido Azuri, Samuel Gelman, Edo Kiper, Daniel Ben Hur, Raviv Dharan, Raya Sorkin, Ziv Porat, Mattia I. Morandi, Neta Regev-Rudzki
The malaria parasite, Plasmodium falciparum, secretes extracellular vesicles (EVs) to facilitate its growth and to communicate with the external microenvironment, primarily targeting the host’s immune cells. How parasitic EVs enter specific immune cell types within the highly heterogeneous pool of immune cells remains largely unknown. Using a combination of imaging flow cytometry and advanced fluorescence analysis, we demonstrated that the route of uptake of parasite-derived EVs differs markedly between host T cells and monocytes. T cells, which are components of the adaptive immune system, internalize parasite-derived EVs mainly through an interaction with the plasma membrane, whereas monocytes, which function in the innate immune system, take up these EVs via endocytosis. The membranal/endocytic balance of EV internalization is driven mostly by the amount of endocytic incorporation. Integrating atomic force microscopy with fluorescence data analysis revealed that internalization depends on the biophysical properties of the cell membrane rather than solely on molecular interactions. In support of this, altering the cholesterol content in the cell membrane tilted the balance in favor of one uptake route over another. Our results provide mechanistic insights into how P. falciparum-derived EVs enter into diverse host cells. This study highlights the sophisticated cell-communication tactics used by the malaria parasite.
{"title":"Host Immune Cell Membrane Deformability Governs the Uptake Route of Malaria-Derived Extracellular Vesicles","authors":"Daniel Alfandari, Irit Rosenhek-Goldian, Ewa Kozela, Reinat Nevo, Marcela Bahlsen Senprún, Anton Moisieiev, Noam Sogauker, Ido Azuri, Samuel Gelman, Edo Kiper, Daniel Ben Hur, Raviv Dharan, Raya Sorkin, Ziv Porat, Mattia I. Morandi, Neta Regev-Rudzki","doi":"10.1021/acsnano.4c07503","DOIUrl":"https://doi.org/10.1021/acsnano.4c07503","url":null,"abstract":"The malaria parasite, <i>Plasmodium falciparum</i>, secretes extracellular vesicles (EVs) to facilitate its growth and to communicate with the external microenvironment, primarily targeting the host’s immune cells. How parasitic EVs enter specific immune cell types within the highly heterogeneous pool of immune cells remains largely unknown. Using a combination of imaging flow cytometry and advanced fluorescence analysis, we demonstrated that the route of uptake of parasite-derived EVs differs markedly between host T cells and monocytes. T cells, which are components of the adaptive immune system, internalize parasite-derived EVs mainly through an interaction with the plasma membrane, whereas monocytes, which function in the innate immune system, take up these EVs via endocytosis. The membranal/endocytic balance of EV internalization is driven mostly by the amount of endocytic incorporation. Integrating atomic force microscopy with fluorescence data analysis revealed that internalization depends on the biophysical properties of the cell membrane rather than solely on molecular interactions. In support of this, altering the cholesterol content in the cell membrane tilted the balance in favor of one uptake route over another. Our results provide mechanistic insights into how <i>P. falciparum</i>-derived EVs enter into diverse host cells. This study highlights the sophisticated cell-communication tactics used by the malaria parasite.","PeriodicalId":21,"journal":{"name":"ACS Nano","volume":"29 1","pages":""},"PeriodicalIF":17.1,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143538643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Substitutional doping in transition-metal dichalcogenides (TMDCs) is a pivotal strategy for tuning their electronic and optical properties, enabling their integration into next-generation electronic and optoelectronic devices. This study examines the critical doping levels at which doped TMDCs transition from nondegenerate to degenerate semiconductors, comparing three-dimensional (3D) bulk TMDCs with their two-dimensional (2D) counterparts. Through systematic characterization of Nb-doped WSe2, we demonstrate that, although high Nb-doped WSe2 bulk samples (Nb density: 3.9 × 1020 cm–3, 2.3% doping level) exhibit degenerate transport behavior, ambipolar behavior emerges at the monolayer limit. This observation highlights a significant increase in the critical doping level upon transitioning from 3D to 2D systems. To elucidate these phenomena, we develop a semiempirical model that incorporates the enhanced dopant ions’ activation energy due to the quantum confinement effect and the modification of the dielectric environment surrounding 2D systems, revealing mechanisms underlying these dimensionality-induced differences. This understanding facilitates the design of doping strategies for high-performance electronic and optoelectronic devices.
{"title":"Dimensionality-Induced Transition from Degenerate to Nondegenerate States in Nb-Doped WSe2","authors":"Kaito Kanahashi, Itsuki Tanaka, Tomonori Nishimura, Kohei Aso, Anh Khoa Augustin Lu, Satoru Morito, Limi Chen, Takafumi Kakeya, Satoshi Watanabe, Yoshifumi Oshima, Yukiko Yamada-Takamura, Keiji Ueno, Amin Azizi, Kosuke Nagashio","doi":"10.1021/acsnano.4c17660","DOIUrl":"https://doi.org/10.1021/acsnano.4c17660","url":null,"abstract":"Substitutional doping in transition-metal dichalcogenides (TMDCs) is a pivotal strategy for tuning their electronic and optical properties, enabling their integration into next-generation electronic and optoelectronic devices. This study examines the critical doping levels at which doped TMDCs transition from nondegenerate to degenerate semiconductors, comparing three-dimensional (3D) bulk TMDCs with their two-dimensional (2D) counterparts. Through systematic characterization of Nb-doped WSe<sub>2</sub>, we demonstrate that, although high Nb-doped WSe<sub>2</sub> bulk samples (Nb density: 3.9 × 10<sup>20</sup> cm<sup>–3</sup>, 2.3% doping level) exhibit degenerate transport behavior, ambipolar behavior emerges at the monolayer limit. This observation highlights a significant increase in the critical doping level upon transitioning from 3D to 2D systems. To elucidate these phenomena, we develop a semiempirical model that incorporates the enhanced dopant ions’ activation energy due to the quantum confinement effect and the modification of the dielectric environment surrounding 2D systems, revealing mechanisms underlying these dimensionality-induced differences. This understanding facilitates the design of doping strategies for high-performance electronic and optoelectronic devices.","PeriodicalId":21,"journal":{"name":"ACS Nano","volume":"5 1","pages":""},"PeriodicalIF":17.1,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143532542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Layered transition metal oxides are some of the most attractive cathode candidates for sodium-ion batteries (SIBs). The main challenge of achieving superior storage performance is to simultaneously boost the ion diffusion kinetics and restrain the undesirable OP4 phase transition upon long-term cycling. In this report, a step-by-step molecule–ion exchange approach is presented to design the high air-stability disordered Ca0.065Na0.55MnO2.05 (CNMO-1) cathode functionalized with an expansive and disordered interlayer microenvironment. Theoretical and experimental investigations revealed that water mediation and ion exchange enhance ion diffusion, while Ca ions stabilize the alkali metal layer, preventing phase transition and manganese (Mn) migration during high-voltage cycling. It exhibits a high specific capacity of 135.4 mA h g–1 at 0.2 A g–1. Beyond that, it can also deliver 81.3 mA h g–1 at the harsh condition of 5 A g–1 with a high 93.3% retention even after 2000 cycles, surpassing most previous achievements. This proposed strategy can be extended to other K+, Zn2+, and La3+ cases, showing an innovative method for designing robust cathodes that enhance the performance of SIBs.
{"title":"Moisture-Resistant, Expansive, and Disordered Interlayer Microenvironment-Enabled Robust Sodium Oxide Cathodes","authors":"Zhouhan Lin, Yanyi Wang, Minfeng Chen, Jianhui Zhu, Zhiyi Xie, Ming Yang, Ning Zhao, Hongwei Mi, Jizhang Chen, Chuanxin He, Dingtao Ma, Peixin Zhang","doi":"10.1021/acsnano.4c17035","DOIUrl":"https://doi.org/10.1021/acsnano.4c17035","url":null,"abstract":"Layered transition metal oxides are some of the most attractive cathode candidates for sodium-ion batteries (SIBs). The main challenge of achieving superior storage performance is to simultaneously boost the ion diffusion kinetics and restrain the undesirable OP4 phase transition upon long-term cycling. In this report, a step-by-step molecule–ion exchange approach is presented to design the high air-stability disordered Ca<sub>0.065</sub>Na<sub>0.55</sub>MnO<sub>2.05</sub> (CNMO-1) cathode functionalized with an expansive and disordered interlayer microenvironment. Theoretical and experimental investigations revealed that water mediation and ion exchange enhance ion diffusion, while Ca ions stabilize the alkali metal layer, preventing phase transition and manganese (Mn) migration during high-voltage cycling. It exhibits a high specific capacity of 135.4 mA h g<sup>–1</sup> at 0.2 A g<sup>–1</sup>. Beyond that, it can also deliver 81.3 mA h g<sup>–1</sup> at the harsh condition of 5 A g<sup>–1</sup> with a high 93.3% retention even after 2000 cycles, surpassing most previous achievements. This proposed strategy can be extended to other K<sup>+</sup>, Zn<sup>2+</sup>, and La<sup>3+</sup> cases, showing an innovative method for designing robust cathodes that enhance the performance of SIBs.","PeriodicalId":21,"journal":{"name":"ACS Nano","volume":"211 1","pages":""},"PeriodicalIF":17.1,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143538646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Zongyou Chen, Keyang Lai, Aoxue Wang, Huayuan Ji, Sha Yu, Zhipeng Fang, Daofeng Liu, Juan Peng, Weihua Lai
The conventional gold nanoparticles (AuNPs) with insufficient brightness face substantial challenges in developing a sensitive lateral flow immunoassay (LFIA). Herein, multibranched manganese–gold (Mn–Au) nanoparticles (MnAuNPs) with a Au core–Mn shell nanostructure were synthesized by a one-pot method. The Mn shell of valence-rich and Au core of high electron transfer efficiency endowed MnAuNPs with oxidase-like activity, which oxidized 3,3′,5,5′-tetramethylbenzidine (TMB) only by electron transfer. Ox-TMB, which was the oxidation product of TMB, is an excellent photothermal agent. Furthermore, the synergistic photothermal effect of ox-TMB and MnAuNPs significantly enhanced the photothermal conversion efficiency. The synergistic photothermal effect of multibranched MnAuNPs and ox-TMB has enabled highly sensitive quantitative detection. The LFIA based on MnAuNPs (cascade LFIA) has achieved sensitive detection of Escherichia coli O157:H7. The entire detection process was completed in 25 min. The limit of detection of cascade LFIA was 239 CFU mL–1, which was 37.21-fold lower than that of AuNPs-LFIA (8892 CFU mL–1). The recoveries of cascade LFIA were 82.63–111.67%, with coefficients of variation of 4.28–14.19%. Overall, this work suggests the potential of MnAuNPs and ox-TMB in the development of sensitive LFIA and broadens the biosensing strategies for point-of-care testing.
{"title":"Electron Transfer-Driven Nanozymes Boost Biosensor Sensitivity via a Synergistic Signal Amplification Strategy","authors":"Zongyou Chen, Keyang Lai, Aoxue Wang, Huayuan Ji, Sha Yu, Zhipeng Fang, Daofeng Liu, Juan Peng, Weihua Lai","doi":"10.1021/acsnano.5c00430","DOIUrl":"https://doi.org/10.1021/acsnano.5c00430","url":null,"abstract":"The conventional gold nanoparticles (AuNPs) with insufficient brightness face substantial challenges in developing a sensitive lateral flow immunoassay (LFIA). Herein, multibranched manganese–gold (Mn–Au) nanoparticles (MnAuNPs) with a Au core–Mn shell nanostructure were synthesized by a one-pot method. The Mn shell of valence-rich and Au core of high electron transfer efficiency endowed MnAuNPs with oxidase-like activity, which oxidized 3,3′,5,5′-tetramethylbenzidine (TMB) only by electron transfer. Ox-TMB, which was the oxidation product of TMB, is an excellent photothermal agent. Furthermore, the synergistic photothermal effect of ox-TMB and MnAuNPs significantly enhanced the photothermal conversion efficiency. The synergistic photothermal effect of multibranched MnAuNPs and ox-TMB has enabled highly sensitive quantitative detection. The LFIA based on MnAuNPs (cascade LFIA) has achieved sensitive detection of <i>Escherichia coli</i> O157:H7. The entire detection process was completed in 25 min. The limit of detection of cascade LFIA was 239 CFU mL<sup>–1</sup>, which was 37.21-fold lower than that of AuNPs-LFIA (8892 CFU mL<sup>–1</sup>). The recoveries of cascade LFIA were 82.63–111.67%, with coefficients of variation of 4.28–14.19%. Overall, this work suggests the potential of MnAuNPs and ox-TMB in the development of sensitive LFIA and broadens the biosensing strategies for point-of-care testing.","PeriodicalId":21,"journal":{"name":"ACS Nano","volume":"5 1","pages":""},"PeriodicalIF":17.1,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143532541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sangwoo Kim, Jeongah Lee, Yong Beom Kim, DongHwan Oh, Jun Kyu Kim, Bonjae Koo, Hyunseung Kim, Gi hong Jung, MinJoong Kim, Gisu Doo, Jongsu Seo, Tae Jin Lim, Kyeounghak Kim, Jeong Woo Han, WooChul Jung
Rational engineering of the surfaces of heterogeneous catalysts (especially the surfaces of supported metals) can endow intriguing catalytic functionalities for electrochemical reactions. However, it often requires complicated steps, and even if it does not, breaking the trade-off between activity and stability is quite challenging. Herein, we present a strategy for reconstructing supported catalysts via in situ growth of metallic nanolayers from the perovskite oxide support. When Ru-coated LaFe0.9Co0.1O3 is thermally reduced, the CoFe nanoalloy spontaneously migrates onto the Ru and greatly increases the physicochemical stability of Ru in alkaline water electrolysis. Benefiting from an 81% reduction in Ru dissolution after decoration, it operates for over 200 h without noticeable degradation. Furthermore, the underlying Ru modifies the electronic structure and surface adsorption properties of the CoFe overlayer toward reaction intermediates, synergistically catalyzing both the oxygen evolution reaction and the hydrogen evolution reaction. Specifically, the mass activity of the oxygen evolution reaction is 64.1 times greater than that of commercial RuO2. Our work highlights a way to protect inherently unstable Ru from dissolution while allowing it to influence surface kinetics from the subsurface sites in heterogeneous catalysts.
{"title":"Enhanced Alkaline Water Electrolysis by the Rational Decoration of RuOx with the In Situ-Grown CoFe Nanolayer","authors":"Sangwoo Kim, Jeongah Lee, Yong Beom Kim, DongHwan Oh, Jun Kyu Kim, Bonjae Koo, Hyunseung Kim, Gi hong Jung, MinJoong Kim, Gisu Doo, Jongsu Seo, Tae Jin Lim, Kyeounghak Kim, Jeong Woo Han, WooChul Jung","doi":"10.1021/acsnano.4c16691","DOIUrl":"https://doi.org/10.1021/acsnano.4c16691","url":null,"abstract":"Rational engineering of the surfaces of heterogeneous catalysts (especially the surfaces of supported metals) can endow intriguing catalytic functionalities for electrochemical reactions. However, it often requires complicated steps, and even if it does not, breaking the trade-off between activity and stability is quite challenging. Herein, we present a strategy for reconstructing supported catalysts via in situ growth of metallic nanolayers from the perovskite oxide support. When Ru-coated LaFe<sub>0.9</sub>Co<sub>0.1</sub>O<sub>3</sub> is thermally reduced, the CoFe nanoalloy spontaneously migrates onto the Ru and greatly increases the physicochemical stability of Ru in alkaline water electrolysis. Benefiting from an 81% reduction in Ru dissolution after decoration, it operates for over 200 h without noticeable degradation. Furthermore, the underlying Ru modifies the electronic structure and surface adsorption properties of the CoFe overlayer toward reaction intermediates, synergistically catalyzing both the oxygen evolution reaction and the hydrogen evolution reaction. Specifically, the mass activity of the oxygen evolution reaction is 64.1 times greater than that of commercial RuO<sub>2</sub>. Our work highlights a way to protect inherently unstable Ru from dissolution while allowing it to influence surface kinetics from the subsurface sites in heterogeneous catalysts.","PeriodicalId":21,"journal":{"name":"ACS Nano","volume":"85 2 1","pages":""},"PeriodicalIF":17.1,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143532674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Constructing subnanometric electrocatalysts is an efficient method to synergistically accelerate H2O dissociation and H+ reduction for pH-universal hydrogen evolution reaction (HER) for industrial water electrolysis to produce green hydrogen. Here, we construct a subnanometric Pt/α-MoC catalyst, where the α-MoC component can dissociate water effectively, with the rapid proton release kinetics of Pt species on Pt/α-MoC to obtain a good HER performance at high current densities in all-pH electrolytes. Quasi-in situ X-ray photoelectron spectroscopy analyses and density functional theory calculations confirm the highly efficient water dissociation capability of α-MoC and the thermodynamically favorable desorption process of hydrolytically dissociated protons on Pt sites at the high current density. Consequently, Pt/α-MoC requires only a low overpotential of 125 mV to achieve a current density of 1000 mA cm–2. Moreover, a Pt/α-MoC-based proton exchange membrane water electrolysis device exhibits a low cell voltage (1.65 V) and promising stability over 300 h with no performance degradation at an industrial-level current density of 1 A cm–2. Notably, even at a current of 100 A, the cell voltage remains low at 2.15 V, demonstrating Pt/α-MoC’s promising potential as a scalable alternative for industrial hydrogen production. These findings elucidate the synergistic mechanism of α-MoC and atomically dispersed Pt in promoting efficient HER, offering valuable guidance for the design of electrocatalysts in high current density hydrogen.
{"title":"Pt/α-MoC Catalyst Boosting pH-Universal Hydrogen Evolution Reaction at High Current Densities","authors":"Wei Liu, Anyang Wang, Jihan Zhang, Shixiang Yu, Maolin Wang, Shuheng Tian, Haoyi Tang, Ziwen Zhao, Xiao Ren, Yuzheng Guo, Ding Ma","doi":"10.1021/acsnano.4c16678","DOIUrl":"https://doi.org/10.1021/acsnano.4c16678","url":null,"abstract":"Constructing subnanometric electrocatalysts is an efficient method to synergistically accelerate H<sub>2</sub>O dissociation and H<sup>+</sup> reduction for pH-universal hydrogen evolution reaction (HER) for industrial water electrolysis to produce green hydrogen. Here, we construct a subnanometric Pt/α-MoC catalyst, where the α-MoC component can dissociate water effectively, with the rapid proton release kinetics of Pt species on Pt/α-MoC to obtain a good HER performance at high current densities in all-pH electrolytes. Quasi-in situ X-ray photoelectron spectroscopy analyses and density functional theory calculations confirm the highly efficient water dissociation capability of α-MoC and the thermodynamically favorable desorption process of hydrolytically dissociated protons on Pt sites at the high current density. Consequently, Pt/α-MoC requires only a low overpotential of 125 mV to achieve a current density of 1000 mA cm<sup>–2</sup>. Moreover, a Pt/α-MoC-based proton exchange membrane water electrolysis device exhibits a low cell voltage (1.65 V) and promising stability over 300 h with no performance degradation at an industrial-level current density of 1 A cm<sup>–2</sup>. Notably, even at a current of 100 A, the cell voltage remains low at 2.15 V, demonstrating Pt/α-MoC’s promising potential as a scalable alternative for industrial hydrogen production. These findings elucidate the synergistic mechanism of α-MoC and atomically dispersed Pt in promoting efficient HER, offering valuable guidance for the design of electrocatalysts in high current density hydrogen.","PeriodicalId":21,"journal":{"name":"ACS Nano","volume":"190 1","pages":""},"PeriodicalIF":17.1,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143538644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lizhi OuYang, Ze Lin, Xi He, Jiaqi Sun, Jiewen Liao, Yuheng Liao, Xudong Xie, Weixian Hu, Ruiyin Zeng, Ranyang Tao, Mengfei Liu, Yun Sun, Bobin Mi, Guohui Liu
Thetreatment of infected wounds is currently a major challenge in clinical medicine, and enhancing antimicrobial and angiogenic capacity is one of the most common strategies. However, the current treatment makes it difficult to balance the antimicrobial effect in the early stage and the angiogenic effect in the later stages of wound healing, leading to an increased rate of poor prognosis. Here, we present a nanoconductive hydrogel EF@S-HGM, consisting of HGM with ECGS, FMLP, and SWCNT. The host–guest supramolecular macromolecule (HGM) hydrogel is biocompatible and can be injected in situ in the wound. The endothelial cell growth factor (ECGS) accelerates vascular remodeling and repairs wounds by promoting the proliferation of endothelial cells. N-Formyl-Met-Leu-Phe (FMLP) recruits neutrophils and increases the antimicrobial capacity. Single-walled carbon nanotubes (SWCNT) make the hydrogel conductive, enabling the hydrogel to utilize the endogenous electric field in the wound to recruit multiple kinds of cells. In addition, we found that the EF@S-HGM hydrogel activates the glucocorticoid receptor senescence pathway and promotes the formation of NET, which enhances the antimicrobial effect. As tissue-engineered skin, the conductive hydrogel EF@S-HGM is a promising material for regenerative medicine that may provide a potential option for the treatment and care of infected wounds and significantly improve patient outcomes and prognosis.
{"title":"Conductive Hydrogel Inspires Neutrophil Extracellular Traps to Combat Bacterial Infections in Wounds","authors":"Lizhi OuYang, Ze Lin, Xi He, Jiaqi Sun, Jiewen Liao, Yuheng Liao, Xudong Xie, Weixian Hu, Ruiyin Zeng, Ranyang Tao, Mengfei Liu, Yun Sun, Bobin Mi, Guohui Liu","doi":"10.1021/acsnano.4c14487","DOIUrl":"https://doi.org/10.1021/acsnano.4c14487","url":null,"abstract":"Thetreatment of infected wounds is currently a major challenge in clinical medicine, and enhancing antimicrobial and angiogenic capacity is one of the most common strategies. However, the current treatment makes it difficult to balance the antimicrobial effect in the early stage and the angiogenic effect in the later stages of wound healing, leading to an increased rate of poor prognosis. Here, we present a nanoconductive hydrogel EF@S-HGM, consisting of HGM with ECGS, FMLP, and SWCNT. The host–guest supramolecular macromolecule (HGM) hydrogel is biocompatible and can be injected in situ in the wound. The endothelial cell growth factor (ECGS) accelerates vascular remodeling and repairs wounds by promoting the proliferation of endothelial cells. N-Formyl-Met-Leu-Phe (FMLP) recruits neutrophils and increases the antimicrobial capacity. Single-walled carbon nanotubes (SWCNT) make the hydrogel conductive, enabling the hydrogel to utilize the endogenous electric field in the wound to recruit multiple kinds of cells. In addition, we found that the EF@S-HGM hydrogel activates the glucocorticoid receptor senescence pathway and promotes the formation of NET, which enhances the antimicrobial effect. As tissue-engineered skin, the conductive hydrogel EF@S-HGM is a promising material for regenerative medicine that may provide a potential option for the treatment and care of infected wounds and significantly improve patient outcomes and prognosis.","PeriodicalId":21,"journal":{"name":"ACS Nano","volume":"62 3 1","pages":""},"PeriodicalIF":17.1,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143539248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Zhaowei Zhang, Xuyang Liu, Chuanyue Peng, Rui Du, Xiaoqin Hong, Jia Xu, Jiaming Chen, Xiaomin Li, Yujing Tang, Yuwei Li, Yang Liu, Chen Xu, Dingbin Liu
Colorectal cancer (CRC) remains a formidable threat to human health, with considerable challenges persisting in its diagnosis, particularly during the early stages of the malignancy. In this study, we elucidated that fecal extracellular vesicle microRNA signatures (FEVOR) could serve as potent noninvasive CRC biomarkers. FEVOR was first revealed by miRNA sequencing, followed by the construction of a CRISPR/Cas13a-based detection platform to interrogate FEVOR expression across a diverse spectrum of clinical cohorts. Machine learning-driven models were subsequently developed within the realms of CRC diagnostics, prognostics, and early warning systems. In a cohort of 38 CRC patients, our diagnostic model achieved an outstanding accuracy of 97.4% (37/38), successfully identifying 37 of 38 CRC cases. This performance significantly outpaced the diagnostic efficacy of two clinically established biomarkers, CEA and CA19-9, which showed accuracies of mere 26.3% (10/38) and 7.9% (3/38), respectively. We also examined the expression levels of FEVOR in several CRC patients both before and after surgery, as well as in patients with colorectal adenomas (CA). Impressively, the results showed that FEVOR could serve as a robust prognostic indicator for CRC and a potential predictor for CA. This endeavor aimed to harness the predictive power of FEVOR for enhancing the precision and efficacy of CRC management paradigms. We envision that these findings will propel both foundational and preclinical research on CRC, as well as clinical studies.
{"title":"Machine Learning-Aided Identification of Fecal Extracellular Vesicle microRNA Signatures for Noninvasive Detection of Colorectal Cancer","authors":"Zhaowei Zhang, Xuyang Liu, Chuanyue Peng, Rui Du, Xiaoqin Hong, Jia Xu, Jiaming Chen, Xiaomin Li, Yujing Tang, Yuwei Li, Yang Liu, Chen Xu, Dingbin Liu","doi":"10.1021/acsnano.4c16698","DOIUrl":"https://doi.org/10.1021/acsnano.4c16698","url":null,"abstract":"Colorectal cancer (CRC) remains a formidable threat to human health, with considerable challenges persisting in its diagnosis, particularly during the early stages of the malignancy. In this study, we elucidated that fecal extracellular vesicle microRNA signatures (FEVOR) could serve as potent noninvasive CRC biomarkers. FEVOR was first revealed by miRNA sequencing, followed by the construction of a CRISPR/Cas13a-based detection platform to interrogate FEVOR expression across a diverse spectrum of clinical cohorts. Machine learning-driven models were subsequently developed within the realms of CRC diagnostics, prognostics, and early warning systems. In a cohort of 38 CRC patients, our diagnostic model achieved an outstanding accuracy of 97.4% (37/38), successfully identifying 37 of 38 CRC cases. This performance significantly outpaced the diagnostic efficacy of two clinically established biomarkers, CEA and CA19-9, which showed accuracies of mere 26.3% (10/38) and 7.9% (3/38), respectively. We also examined the expression levels of FEVOR in several CRC patients both before and after surgery, as well as in patients with colorectal adenomas (CA). Impressively, the results showed that FEVOR could serve as a robust prognostic indicator for CRC and a potential predictor for CA. This endeavor aimed to harness the predictive power of FEVOR for enhancing the precision and efficacy of CRC management paradigms. We envision that these findings will propel both foundational and preclinical research on CRC, as well as clinical studies.","PeriodicalId":21,"journal":{"name":"ACS Nano","volume":"85 1","pages":""},"PeriodicalIF":17.1,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143538645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Darcey Ridgway-Brown, Anna S. Leathard, Oliver France, Stephen P. Muench, Michael E. Webb, Lars J. C. Jeuken, Peter J. F. Henderson, Annette F. Taylor, Paul A. Beales
Understanding ion transport dynamics in reactive vesicles is pivotal for exploring biological and chemical processes and essential for designing synthetic cells. In this work, we investigate how proton transport and membrane potential regulate pH dynamics in an autocatalytic enzyme reaction within lipid vesicles. Combining experimental and numerical methods, we demonstrate that compartmentalization within lipid membranes accelerates internal reactions, attributed to protection from the external acidic environment. In experiments, we explored how proton movement significantly impacts internal reactions by changing bilayer thickness, adding ion transporters, and varying buffers. Numerical investigations incorporated electrical membrane potential and capacitance into a kinetic model of the process, elucidating the mechanisms that dictate the control of reaction time observed in the experiment, driven by both electrical and chemical potential gradients. These findings establish a framework for controlling pH clock reactions via membrane changes and targeted manipulation of proton movement, which could aid in the design of synthetic cells with precise, controlled functionalities.
{"title":"Membrane Transport Modulates the pH-Regulated Feedback of an Enzyme Reaction Confined within Lipid Vesicles","authors":"Darcey Ridgway-Brown, Anna S. Leathard, Oliver France, Stephen P. Muench, Michael E. Webb, Lars J. C. Jeuken, Peter J. F. Henderson, Annette F. Taylor, Paul A. Beales","doi":"10.1021/acsnano.4c13048","DOIUrl":"https://doi.org/10.1021/acsnano.4c13048","url":null,"abstract":"Understanding ion transport dynamics in reactive vesicles is pivotal for exploring biological and chemical processes and essential for designing synthetic cells. In this work, we investigate how proton transport and membrane potential regulate pH dynamics in an autocatalytic enzyme reaction within lipid vesicles. Combining experimental and numerical methods, we demonstrate that compartmentalization within lipid membranes accelerates internal reactions, attributed to protection from the external acidic environment. In experiments, we explored how proton movement significantly impacts internal reactions by changing bilayer thickness, adding ion transporters, and varying buffers. Numerical investigations incorporated electrical membrane potential and capacitance into a kinetic model of the process, elucidating the mechanisms that dictate the control of reaction time observed in the experiment, driven by both electrical and chemical potential gradients. These findings establish a framework for controlling pH clock reactions via membrane changes and targeted manipulation of proton movement, which could aid in the design of synthetic cells with precise, controlled functionalities.","PeriodicalId":21,"journal":{"name":"ACS Nano","volume":"2 1","pages":""},"PeriodicalIF":17.1,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143539281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Chern insulator (CI) exhibits rich physics with great interest in both theory and experiment. Here, we focus on the honeycomb and kagome ferromagnets and demonstrate that coupling the nontrivial electronic and magnonic bands allows for the dual CIs, where the quantum anomalous Hall effect and its bosonic analogue, i.e., topological magnon insulator, appear simultaneously within one two-dimensional (2D) ferromagnet. Both the tight-binding model and Heisenberg-DM model are constructed to demonstrate the feasibility of attaining the dual CIs and point out the perspectives of dual CIs for spin-orbit coupling and the Dzyaloshinskii-Moriya interaction. Moreover, based on Chern number, topological quantum chemistry, and edge state analysis, we explore the emergence and stability of such dual CIs in realistic 2D ferromagnets, which is expected to draw great experimental attentions.
{"title":"Dual Chern Insulators with Electronic and Magnonic Edge States in Two-Dimensional Ferromagnets","authors":"Yingxi Bai, Xiaorong Zou, Zhiqi Chen, Runhan Li, Bo Yuan, Ying Dai, Baibiao Huang, Chengwang Niu","doi":"10.1021/acsnano.5c00323","DOIUrl":"https://doi.org/10.1021/acsnano.5c00323","url":null,"abstract":"Chern insulator (CI) exhibits rich physics with great interest in both theory and experiment. Here, we focus on the honeycomb and kagome ferromagnets and demonstrate that coupling the nontrivial electronic and magnonic bands allows for the dual CIs, where the quantum anomalous Hall effect and its bosonic analogue, i.e., topological magnon insulator, appear simultaneously within one two-dimensional (2D) ferromagnet. Both the tight-binding model and Heisenberg-DM model are constructed to demonstrate the feasibility of attaining the dual CIs and point out the perspectives of dual CIs for spin-orbit coupling and the Dzyaloshinskii-Moriya interaction. Moreover, based on Chern number, topological quantum chemistry, and edge state analysis, we explore the emergence and stability of such dual CIs in realistic 2D ferromagnets, which is expected to draw great experimental attentions.","PeriodicalId":21,"journal":{"name":"ACS Nano","volume":"90 1","pages":""},"PeriodicalIF":17.1,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143532536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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