Evaluation anti-inflammatory Barleria by in Abstract The methanol extract of Barleria Cristata leaves (BCM) was evaluated for anti-inflammatory activity using in vivo and in vitro methods. In the in vivo inflammation tests, BCM significantly inhibited edema produced by histamine and serotonin in rats, also reduces significantly acetic acid-induced vascular permeability in mice dose dependently. In the in vitro tests, the probable supporting mode by which BCM mediates its effects on inflammatory conditions was studied on red blood cells (RBC’s) exposed to hypotonic solution and thermally induced protein denaturation. BCM exhibited significant membrane-stabilizing property. Thermal induced protein denaturation was significantly inhibited by the extract. The effect was compared with the activity of indomethacin and cyproheptadine as reference standard against different types of inflammation. Results of the study revealed that BCM possesses significant anti-inflammatory activity.
{"title":"Evaluation of anti-inflammatory activity of methanol extract of Barleria Cristata leaves by in vivo and in vitro methods","authors":"M. Gambhire, A. Juvekar, S. Wankhede","doi":"10.5580/28ac","DOIUrl":"https://doi.org/10.5580/28ac","url":null,"abstract":"Evaluation anti-inflammatory Barleria by in Abstract The methanol extract of Barleria Cristata leaves (BCM) was evaluated for anti-inflammatory activity using in vivo and in vitro methods. In the in vivo inflammation tests, BCM significantly inhibited edema produced by histamine and serotonin in rats, also reduces significantly acetic acid-induced vascular permeability in mice dose dependently. In the in vitro tests, the probable supporting mode by which BCM mediates its effects on inflammatory conditions was studied on red blood cells (RBC’s) exposed to hypotonic solution and thermally induced protein denaturation. BCM exhibited significant membrane-stabilizing property. Thermal induced protein denaturation was significantly inhibited by the extract. The effect was compared with the activity of indomethacin and cyproheptadine as reference standard against different types of inflammation. Results of the study revealed that BCM possesses significant anti-inflammatory activity.","PeriodicalId":22523,"journal":{"name":"The Internet Journal of Pharmacology","volume":"51 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2008-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86268817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Organic solvents such as acetone, ethyl acetate, isopropyl alcohol, methanol, tetrahydrofuran and toluene frequently used in pharmaceutical industry for the manufacturing of Active Pharmaceutical ingredients (APIs). GMP conditions commands to control adequately the quality of APIs. A selective Gas Chromatographic (GC) method has been developed and validated as per ICH guidelines for residual solvent analysis in 16 different APIs. Residual solvents in APIs were monitored using gas chromatography (GC) with Flame Ionisation detector (FID). The separation was carried out on BP 624 column (30m X 0.53mm i.d. X 0.25mm coating thickness), using GC 17 A Shimadzu, with nitrogen as carrier gas in the split mode by direct injection method. The method described is simple, sensitive, rugged, reliable and reproducible for the quantitation of acetone, ethyl acetate, isopropyl alcohol, methanol, tetrahydrofuran and toluene at residual level from intermediates and APIs.
有机溶剂，如丙酮、乙酸乙酯、异丙醇、甲醇、四氢呋喃和甲苯，经常用于制药工业中制造活性药物成分(api)。GMP要求充分控制原料药的质量。根据ICH指南，开发并验证了一种选择性气相色谱(GC)方法，用于16种不同原料药的残留溶剂分析。采用气相色谱法(GC)和火焰电离检测器(FID)对原料药中的残留溶剂进行了监测。色谱柱为BP 624柱(直径30m X 0.53mm，膜厚0.25mm)，色谱柱为GC 17a Shimadzu，载气为氮气，采用直接进样分离。本方法简便、灵敏、可靠、重现性好，适用于中间体和原料药中丙酮、乙酸乙酯、异丙醇、甲醇、四氢呋喃和甲苯残留量的定量。
{"title":"Determination of Organic Volatile Impurities in Active Pharmaceutical Ingredients","authors":"S. Puranik, P. Pai, G. Rao","doi":"10.5580/356","DOIUrl":"https://doi.org/10.5580/356","url":null,"abstract":"Organic solvents such as acetone, ethyl acetate, isopropyl alcohol, methanol, tetrahydrofuran and toluene frequently used in pharmaceutical industry for the manufacturing of Active Pharmaceutical ingredients (APIs). GMP conditions commands to control adequately the quality of APIs. A selective Gas Chromatographic (GC) method has been developed and validated as per ICH guidelines for residual solvent analysis in 16 different APIs. Residual solvents in APIs were monitored using gas chromatography (GC) with Flame Ionisation detector (FID). The separation was carried out on BP 624 column (30m X 0.53mm i.d. X 0.25mm coating thickness), using GC 17 A Shimadzu, with nitrogen as carrier gas in the split mode by direct injection method. The method described is simple, sensitive, rugged, reliable and reproducible for the quantitation of acetone, ethyl acetate, isopropyl alcohol, methanol, tetrahydrofuran and toluene at residual level from intermediates and APIs.","PeriodicalId":22523,"journal":{"name":"The Internet Journal of Pharmacology","volume":"75 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2008-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82292676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Ghosh, Isha S. Dhande, V. Kakade, R. Vohra, V. Kadam, Mehra
Madhuca longifolia commonly known as theButter nut treeis used traditionally in the Indian folk medicine for the treatment of diabetes mellitus. The hydroethanolic extract of the leaves of Madhuca longifolia was administered orally to alloxaninduced diabetic rats and investigated for its antidiabetic properties. Administration of 150 mg/kg and 300 mg/kg extract (once a day, for thirty consecutive days) significantly lowered blood glucose levels. Furthermore, the activity of glucose-6-phosphate dehydrogenase, serum triglycerides, HDL and total cholesterol levels showed marked improvement which indicates that the hydroethanolic extract possesses antihyperglycemic activity.
{"title":"Antihyperglycemic activity of Madhuca longifolia in alloxan -induced diabetic rats","authors":"R. Ghosh, Isha S. Dhande, V. Kakade, R. Vohra, V. Kadam, Mehra","doi":"10.5580/a1c","DOIUrl":"https://doi.org/10.5580/a1c","url":null,"abstract":"Madhuca longifolia commonly known as theButter nut treeis used traditionally in the Indian folk medicine for the treatment of diabetes mellitus. The hydroethanolic extract of the leaves of Madhuca longifolia was administered orally to alloxaninduced diabetic rats and investigated for its antidiabetic properties. Administration of 150 mg/kg and 300 mg/kg extract (once a day, for thirty consecutive days) significantly lowered blood glucose levels. Furthermore, the activity of glucose-6-phosphate dehydrogenase, serum triglycerides, HDL and total cholesterol levels showed marked improvement which indicates that the hydroethanolic extract possesses antihyperglycemic activity.","PeriodicalId":22523,"journal":{"name":"The Internet Journal of Pharmacology","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2008-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81904762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The effects of Ascars Suum (A. Suum) extract on gastric acid secretion were investigated in urethane-anaesthetised rats. Three determinations were done viz (1) the acid content when no drug was administered (2) the acid content when histamine was injected and (3) the acid content when the A. Suum extract was given alone or after histamine was administered. The extract (5.5 or 14mg/kg) reduced histamine-induced gastric acid secretion to the control value. At 14mg/kg the extract also reduced basal gastric acid secretion. At the peak of histamine-induced gastric acid secretion, the administration of the extract promptly reduced the gastric acid secretion to basal values. These results indicate that extracts of Ascaris Suum inhibit gastric acid secretion.
{"title":"Inhibitory effects of ascaris suum extract On gastric acid secretion in rats","authors":"R. Nwankwoala, O. Georgewill, U. Georgewill","doi":"10.5580/26c4","DOIUrl":"https://doi.org/10.5580/26c4","url":null,"abstract":"The effects of Ascars Suum (A. Suum) extract on gastric acid secretion were investigated in urethane-anaesthetised rats. Three determinations were done viz (1) the acid content when no drug was administered (2) the acid content when histamine was injected and (3) the acid content when the A. Suum extract was given alone or after histamine was administered. The extract (5.5 or 14mg/kg) reduced histamine-induced gastric acid secretion to the control value. At 14mg/kg the extract also reduced basal gastric acid secretion. At the peak of histamine-induced gastric acid secretion, the administration of the extract promptly reduced the gastric acid secretion to basal values. These results indicate that extracts of Ascaris Suum inhibit gastric acid secretion.","PeriodicalId":22523,"journal":{"name":"The Internet Journal of Pharmacology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2008-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90433430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The aim of the present study is to evaluate the hypoglycemic action of ethanolic extract of seed kernel of Caesalpinia bonducella Fleming on normal and alloxan-induced diabetic albino rats. The ethanolic extract (200mg/kg/d) was administered orally for two weeks to alloxan-induced diabetic rats. Blood glucose was estimated every week for two consecutive weeks along with body weight monitoring. For evaluation of mechanism of action of test drug, glycogen estimation was done in liver, heart and skeletal muscle and effect on adrenaline-induced hyperglycemia was seen. The test drug significantly (p<0.05) reduced the rise in blood glucose induced by alloxan. The test drug produced significant (p<0.05) increase in liver glycogen and also significantly (p<0.05) reduced adrenaline-induced hyperglycaemia. Significant (p<0.05) lowering of normal blood glucose was also found. Thus, the seed kernel of Caesalpinia bonducella has significant antidiabetic and hypoglycemic activity.
{"title":"Hypoglycemic Action of Seed Kernel of Caesalpinia bonducella Fleming In Normal and Alloxan- Induced Diabetic Albino Rats","authors":"G. Sarma, Swarnamoni Das","doi":"10.5580/19d1","DOIUrl":"https://doi.org/10.5580/19d1","url":null,"abstract":"The aim of the present study is to evaluate the hypoglycemic action of ethanolic extract of seed kernel of Caesalpinia bonducella Fleming on normal and alloxan-induced diabetic albino rats. The ethanolic extract (200mg/kg/d) was administered orally for two weeks to alloxan-induced diabetic rats. Blood glucose was estimated every week for two consecutive weeks along with body weight monitoring. For evaluation of mechanism of action of test drug, glycogen estimation was done in liver, heart and skeletal muscle and effect on adrenaline-induced hyperglycemia was seen. The test drug significantly (p<0.05) reduced the rise in blood glucose induced by alloxan. The test drug produced significant (p<0.05) increase in liver glycogen and also significantly (p<0.05) reduced adrenaline-induced hyperglycaemia. Significant (p<0.05) lowering of normal blood glucose was also found. Thus, the seed kernel of Caesalpinia bonducella has significant antidiabetic and hypoglycemic activity.","PeriodicalId":22523,"journal":{"name":"The Internet Journal of Pharmacology","volume":"51 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2008-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88662623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Srujan Kumar, S. M. Asdaq, N. P. Kumar, M. Asad, D. K. Khajuria
Aim of the study: The aim of this study was to investigate the hepatoprotective effect of methanolic extract of Zizyphus jujuba fruits (MEZJ), in rat models of paracetamol (PCM) and thioacetamide (TAA) induced hepatic damage.Materials and Methods: Sprague-Dawely rats were prophylactically treated with three dose of MEZJ (1000, 500 and 250 mg/kg, p.o) for 10 days and subsequently liver damage was induced. Hepatoprotective potential was evaluated by measuring biomarkers and antioxidants.Results: The low and medium doses of MEZJ significantly inhibited the acute elevation of biomarkers in serum and elevated the fall of biomarkers in liver tissue homogenate (LTH). The activities of antioxidants enzymes were significantly increased in LTH of rats pretreated with low and medium doses of MEZJ. Results of histopathological studies supported the biochemical findings. However, high dose of MEZJ was less effective than low and medium doses.Conclusion: It was concluded that MEZJ possesses hepatoprotective activity probably due to its antioxidant effect.
{"title":"Protective Effect Of Zizyphus Jujuba Fruit Extract Against Paracetamol And Thioacetamide Induced Hepatic Damage In Rats","authors":"Srujan Kumar, S. M. Asdaq, N. P. Kumar, M. Asad, D. K. Khajuria","doi":"10.5580/2991","DOIUrl":"https://doi.org/10.5580/2991","url":null,"abstract":"Aim of the study: The aim of this study was to investigate the hepatoprotective effect of methanolic extract of Zizyphus jujuba fruits (MEZJ), in rat models of paracetamol (PCM) and thioacetamide (TAA) induced hepatic damage.Materials and Methods: Sprague-Dawely rats were prophylactically treated with three dose of MEZJ (1000, 500 and 250 mg/kg, p.o) for 10 days and subsequently liver damage was induced. Hepatoprotective potential was evaluated by measuring biomarkers and antioxidants.Results: The low and medium doses of MEZJ significantly inhibited the acute elevation of biomarkers in serum and elevated the fall of biomarkers in liver tissue homogenate (LTH). The activities of antioxidants enzymes were significantly increased in LTH of rats pretreated with low and medium doses of MEZJ. Results of histopathological studies supported the biochemical findings. However, high dose of MEZJ was less effective than low and medium doses.Conclusion: It was concluded that MEZJ possesses hepatoprotective activity probably due to its antioxidant effect.","PeriodicalId":22523,"journal":{"name":"The Internet Journal of Pharmacology","volume":"48 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2008-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85029001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In the present study diuretic activity of Diur-08, a polyherbal formulation was studied. The animals were divided into three groups of six animals each. All the animals received priming dose of 0.9% sodium chloride solution (25 ml/Kg body weight.). The first group served as control and the second group received the standard drug Spiranolactone (20 mg/Kg body weight) in 0.9% sodium chloride solution. The other group received Diur-08 at a dose of 150mg/Kg body weight suspended in 0.9% sodium chloride solution (post oral.).Urine volume was measured for all the groups for 5h. Urinary levels of sodium, potassium and chloride were estimated. Both spironolactone and formulation Diur-08 treated animals shown significant diuretic activity compared to control animals. Also there is significant increase in Na and Cl excretion in treated animals when compared to control animals. We observed a potent diuretic and electrolyte excretion activity of Diur-08 formulation.
{"title":"Evaluation of Diur-08 A Polyherbal Formulation for Diuretic Activity","authors":"M. Shenoy, C. Shastry","doi":"10.5580/cba","DOIUrl":"https://doi.org/10.5580/cba","url":null,"abstract":"In the present study diuretic activity of Diur-08, a polyherbal formulation was studied. The animals were divided into three groups of six animals each. All the animals received priming dose of 0.9% sodium chloride solution (25 ml/Kg body weight.). The first group served as control and the second group received the standard drug Spiranolactone (20 mg/Kg body weight) in 0.9% sodium chloride solution. The other group received Diur-08 at a dose of 150mg/Kg body weight suspended in 0.9% sodium chloride solution (post oral.).Urine volume was measured for all the groups for 5h. Urinary levels of sodium, potassium and chloride were estimated. Both spironolactone and formulation Diur-08 treated animals shown significant diuretic activity compared to control animals. Also there is significant increase in Na and Cl excretion in treated animals when compared to control animals. We observed a potent diuretic and electrolyte excretion activity of Diur-08 formulation.","PeriodicalId":22523,"journal":{"name":"The Internet Journal of Pharmacology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2008-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83090261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Keeping in view of the biological potential of Schiff bases attached to heterocyclic ring system the synthesis of certain Schiff bases containing 1,8-naphthyridines were undertaken.2-amino nicotinaldehyde on condensation with ethyl cyano acetate yielded 2-hydroxy-3-cyano-1,8-naphthyridine.(I) This upon treatment with 10% NaoH solution gave 2-hydroxy-1,8naphthyridine-3-carboxylic acid. (II). Compound II on treatment with phosphorus oxy chloride gave 2-chloro-1,8-naphthyridine-3carboxylic acid.(III). The compound (III) upon treatment with hydrazine hydrate in ethanol gave 2-hydrazido-1,8-naphthyridine-3carboxylic acid. (Va-d) which are schiff’s bases. The schiff’s bases on treatment with trietyhl amine in dry 1,4-dioxan and mono chloro acetyl chloride yielded 3-chloro-4-(substituted phenyl)-1-(3-carboxy-1,8-naphthyridin-2-yl amino)-azetidin-2-one.(Via-d). The constitution of all compounds synthesized was established by elemental analysis and spectral studies. Allcompounds were evaluated for antibacterial and antifungal activites against different strains of bacterial and fungal organisms. Some of the compounds exhibited significant anti bacterial activity.
{"title":"Synthesis And Anti Microbial Studies Of Certain Schiff Bases Containing 1,8-Naphthyridine Moiety","authors":"B. Vinod, P. M. Kumar, V. Prashanth, I. Baskar","doi":"10.5580/1e5b","DOIUrl":"https://doi.org/10.5580/1e5b","url":null,"abstract":"Keeping in view of the biological potential of Schiff bases attached to heterocyclic ring system the synthesis of certain Schiff bases containing 1,8-naphthyridines were undertaken.2-amino nicotinaldehyde on condensation with ethyl cyano acetate yielded 2-hydroxy-3-cyano-1,8-naphthyridine.(I) This upon treatment with 10% NaoH solution gave 2-hydroxy-1,8naphthyridine-3-carboxylic acid. (II). Compound II on treatment with phosphorus oxy chloride gave 2-chloro-1,8-naphthyridine-3carboxylic acid.(III). The compound (III) upon treatment with hydrazine hydrate in ethanol gave 2-hydrazido-1,8-naphthyridine-3carboxylic acid. (Va-d) which are schiff’s bases. The schiff’s bases on treatment with trietyhl amine in dry 1,4-dioxan and mono chloro acetyl chloride yielded 3-chloro-4-(substituted phenyl)-1-(3-carboxy-1,8-naphthyridin-2-yl amino)-azetidin-2-one.(Via-d). The constitution of all compounds synthesized was established by elemental analysis and spectral studies. Allcompounds were evaluated for antibacterial and antifungal activites against different strains of bacterial and fungal organisms. Some of the compounds exhibited significant anti bacterial activity.","PeriodicalId":22523,"journal":{"name":"The Internet Journal of Pharmacology","volume":"35 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2008-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79533515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Kshirsagar, Y. Vetal, P. Ashok, Pradnya A. Bhosle, D. Ingawale
Liver, the largest organ in the body is being evolved to maintain the body’s internal milieu and also protect itself from the challenges it faces during its functioning. It is a vital organ having diverse functions. It plays an important role not only in the metabolism, synthesis and storage but also in the detoxification of many endogenous and exogenous compounds and converting them to less toxic substances for excretion. Hepatotoxicity implies chemical-driven liver damage. Certain medicinal agents when taken in overdoses and sometimes even when introduced within therapeutic ranges may injure the liver. The liver plays a central role in transforming and clearing chemicals and is susceptible to the toxicity from these agents. The present review provides an overview of various drugs causing hepatotoxicity, various types of drug induced hepatotoxicity and their mechanisms.
{"title":"Drug Induced Hepatotoxicity: A Comprehensive Review","authors":"A. Kshirsagar, Y. Vetal, P. Ashok, Pradnya A. Bhosle, D. Ingawale","doi":"10.5580/de4","DOIUrl":"https://doi.org/10.5580/de4","url":null,"abstract":"Liver, the largest organ in the body is being evolved to maintain the body’s internal milieu and also protect itself from the challenges it faces during its functioning. It is a vital organ having diverse functions. It plays an important role not only in the metabolism, synthesis and storage but also in the detoxification of many endogenous and exogenous compounds and converting them to less toxic substances for excretion. Hepatotoxicity implies chemical-driven liver damage. Certain medicinal agents when taken in overdoses and sometimes even when introduced within therapeutic ranges may injure the liver. The liver plays a central role in transforming and clearing chemicals and is susceptible to the toxicity from these agents. The present review provides an overview of various drugs causing hepatotoxicity, various types of drug induced hepatotoxicity and their mechanisms.","PeriodicalId":22523,"journal":{"name":"The Internet Journal of Pharmacology","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2008-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79186884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Martha, U. Veldandi, K. Devarakonda, N. Pantam, Surender Thungathurthi
In the present study, we made an attempt to investigate role of insulin like growth factor-I (IGF-I) in aspirin pretreatment in streptozotocin induced type-2 diabetes mellitus in rats. Rat pups were divided in to four groups, on 5th day of their birth, group-I pups were received citrate buffer solution served as normal, group-II were treated only with streptozotocin (80mg/kg, i.p) served as diabetic, group-III & group-IV were treated with aspirin (10mg/kg/day, p.o) for one month (5-35 days) and two month (5-65) after streptozotocin served as treated groups. On 36 and 66 day, blood samples were collected from all animals and fasting blood sugar, fasting insulin, IGF-I, insulin resistance and insulin sensitivity levels were estimated. Results of 36 & 66 days blood samples of pups treated with streptozotocin alone and in combination with aspirin for one month and two months were shown significantly raised body weight, fasting blood glucose and insulin resistance levels (P=0.0005, p<.0001, p<.0001, P=0.0006, p<.0001, P=0.0030) and significantly lowered fasting insulin and insulin sensitivity levels when compared to the normal control pups (p<.0001, p<.0001, p<.0001, p<.0001, p<.0001, P=0.0068) respectively. Pups treated with aspirin for one month were shown significantly raised IGF-I levels but two months treatment were shown significantly lowered IGF-I levels when compared to the normal pups (p<.0001). The present study indicates that aspirin pretreatment seems to protect pancreas from damage caused by STZ and maintains glucose levels in diabetic rats and increases insulin sensitivity and reduces insulin resistance, this may a involvement of insulin like pathway particularly IGF-I.
本研究旨在探讨胰岛素样生长因子- i (IGF-I)在链脲佐菌素诱导的2型糖尿病大鼠阿司匹林预处理中的作用。将大鼠仔鼠分为4组，出生第5天给予枸橼酸缓冲液，ⅰ组作为正常对照组，ⅱ组作为糖尿病组仅给予链脲佐菌素(80mg/kg，每日1次)治疗，ⅲ组和ⅳ组给予阿司匹林(10mg/kg/d，每日1次)治疗1个月(5-35天)和2个月(5-65天)。在第36天和第66天采集所有动物的血液样本，评估空腹血糖、空腹胰岛素、IGF-I、胰岛素抵抗和胰岛素敏感性水平。单用链脲佐菌素和联用阿司匹林治疗1个月和2个月的36天和66天的血液样本显示，幼犬体重、空腹血糖和胰岛素抵抗水平显著升高(P=0.0005, P <)。0001, p <。0001, P=0.0006, P <。0001, P=0.0030)，与正常对照幼崽相比，空腹胰岛素和胰岛素敏感性水平显著降低(P < 0.001)。0001, p <。0001, p <。0001, p <。0001, p <。0001, P=0.0068)。与正常幼犬相比，服用阿司匹林一个月的幼犬IGF-I水平显著升高，但服用阿司匹林两个月的幼犬IGF-I水平显著降低(p< 0.0001)。本研究提示阿司匹林预处理似乎可以保护胰腺免受STZ损伤，维持糖尿病大鼠的葡萄糖水平，增加胰岛素敏感性，降低胰岛素抵抗，这可能与胰岛素样通路特别是IGF-I有关。
{"title":"Role of IGF-I in aspirin pretreatment in streptozotocin induced type-II diabetic rats","authors":"S. Martha, U. Veldandi, K. Devarakonda, N. Pantam, Surender Thungathurthi","doi":"10.5580/74","DOIUrl":"https://doi.org/10.5580/74","url":null,"abstract":"In the present study, we made an attempt to investigate role of insulin like growth factor-I (IGF-I) in aspirin pretreatment in streptozotocin induced type-2 diabetes mellitus in rats. Rat pups were divided in to four groups, on 5th day of their birth, group-I pups were received citrate buffer solution served as normal, group-II were treated only with streptozotocin (80mg/kg, i.p) served as diabetic, group-III & group-IV were treated with aspirin (10mg/kg/day, p.o) for one month (5-35 days) and two month (5-65) after streptozotocin served as treated groups. On 36 and 66 day, blood samples were collected from all animals and fasting blood sugar, fasting insulin, IGF-I, insulin resistance and insulin sensitivity levels were estimated. Results of 36 & 66 days blood samples of pups treated with streptozotocin alone and in combination with aspirin for one month and two months were shown significantly raised body weight, fasting blood glucose and insulin resistance levels (P=0.0005, p<.0001, p<.0001, P=0.0006, p<.0001, P=0.0030) and significantly lowered fasting insulin and insulin sensitivity levels when compared to the normal control pups (p<.0001, p<.0001, p<.0001, p<.0001, p<.0001, P=0.0068) respectively. Pups treated with aspirin for one month were shown significantly raised IGF-I levels but two months treatment were shown significantly lowered IGF-I levels when compared to the normal pups (p<.0001). The present study indicates that aspirin pretreatment seems to protect pancreas from damage caused by STZ and maintains glucose levels in diabetic rats and increases insulin sensitivity and reduces insulin resistance, this may a involvement of insulin like pathway particularly IGF-I.","PeriodicalId":22523,"journal":{"name":"The Internet Journal of Pharmacology","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2008-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81157600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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