Pub Date : 2017-10-28DOI: 10.36233/0372-9311-2017-5-39-45
L. Solntsev, V. Dubyansky
Цель. Зонирование территории Нижегородской области по риску заражения ГЛПС с использованием метода Maxent. Материалы и методы. Материалами являлись данные Центра гигиены и эпидемиологии по Нижегородской области по каждому случаю зара жения ГЛ ПС за 2010 — 2016 гг.; данные по условиям окружающей среды (Bioclim); данные по вегетационной активности (MODIS). Обработка проводилась в пакетах ArcGIS 10.2.2 и Maxent 3.3.Зк. Результаты. Получена и валидирована модель для оценки потенциаль ного риска заражения ГЛПС на территории Нижегородской области. Заключение. Полученные результаты не противоречат фактически наблюдаемой пространственной локализации случаев заражения ГЛПС (точность предсказания составляет более 75%), выявляют связь между пространственной локализацией случаев заражения ГЛПС и со четания факторов среды и позволяют формировать прогнозы изменения границ потен циально опасных участков при изменении факторов среды.
{"title":"EXPERIENCE OF USING MAXIMAL ENTROPY METHOD (MAXENT) FOR ZONING OF THE TERRITORY BY HERS RISK USING NIZHNY NOVGOROD REGION AS AN EXAMPLE","authors":"L. Solntsev, V. Dubyansky","doi":"10.36233/0372-9311-2017-5-39-45","DOIUrl":"https://doi.org/10.36233/0372-9311-2017-5-39-45","url":null,"abstract":"Цель. Зонирование территории Нижегородской области по риску заражения ГЛПС с использованием метода Maxent. Материалы и методы. Материалами являлись данные Центра гигиены и эпидемиологии по Нижегородской области по каждому случаю зара жения ГЛ ПС за 2010 — 2016 гг.; данные по условиям окружающей среды (Bioclim); данные по вегетационной активности (MODIS). Обработка проводилась в пакетах ArcGIS 10.2.2 и Maxent 3.3.Зк. Результаты. Получена и валидирована модель для оценки потенциаль ного риска заражения ГЛПС на территории Нижегородской области. Заключение. Полученные результаты не противоречат фактически наблюдаемой пространственной локализации случаев заражения ГЛПС (точность предсказания составляет более 75%), выявляют связь между пространственной локализацией случаев заражения ГЛПС и со четания факторов среды и позволяют формировать прогнозы изменения границ потен циально опасных участков при изменении факторов среды.","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":"10 1","pages":"39-45"},"PeriodicalIF":0.0,"publicationDate":"2017-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88628705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-10-28DOI: 10.36233/0372-9311-2017-5-91-100
V. A. Markin, D. Chifanov
and the of of the the At there is a significant expansion of the ranges of some zooantroponozes pathogens, filovirus Ebola and arboviruses — Rift Valley fever, Chikungunya. When extending the boundaries of epidemic foci in the new territory for the pathogen, can occur aggravating of clinical manifestations of the disease and increase mortality among the indigenous population. Extremely hazardous exotic viral hemorrhagic fever (Ebola, Lassa) when transfer with sick people in some cases, can cause contamination of the contact persons. Rift hlley fever — one of the most aggressive arboviruses, in the case of importa tion can form stable epidemic foci. Transfer of Zika fever in the territory of the substantial epidemiological value. Epidemiological factors, essential for the formation of new areas of pathogens the presence of permissive candidates in In parts of Africa and Asia, with soils poor in selenium, were first introduced pathogens or highly virulent strains of influenza A, SARS, Ebola and of SIV, and drifts on these and similar areas have led to an increase in the virulence of viruses.
{"title":"TRANSFER OF DANGEROUS VIRAL INFECTIONS IN THE NON-ENDEMIC AREA","authors":"V. A. Markin, D. Chifanov","doi":"10.36233/0372-9311-2017-5-91-100","DOIUrl":"https://doi.org/10.36233/0372-9311-2017-5-91-100","url":null,"abstract":"and the of of the the At there is a significant expansion of the ranges of some zooantroponozes pathogens, filovirus Ebola and arboviruses — Rift Valley fever, Chikungunya. When extending the boundaries of epidemic foci in the new territory for the pathogen, can occur aggravating of clinical manifestations of the disease and increase mortality among the indigenous population. Extremely hazardous exotic viral hemorrhagic fever (Ebola, Lassa) when transfer with sick people in some cases, can cause contamination of the contact persons. Rift hlley fever — one of the most aggressive arboviruses, in the case of importa tion can form stable epidemic foci. Transfer of Zika fever in the territory of the substantial epidemiological value. Epidemiological factors, essential for the formation of new areas of pathogens the presence of permissive candidates in In parts of Africa and Asia, with soils poor in selenium, were first introduced pathogens or highly virulent strains of influenza A, SARS, Ebola and of SIV, and drifts on these and similar areas have led to an increase in the virulence of viruses.","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":"48 1","pages":"91-100"},"PeriodicalIF":0.0,"publicationDate":"2017-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74937512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-10-28DOI: 10.36233/0372-9311-2017-5-78-90
L. Somova, N. G. Plekhova, E. Drobot, I. Lyapun
{"title":"PSEUDOTUBERCULOSIS: PATHOGENETIC VALUE OF INNATE IMMUNITY CELLS","authors":"L. Somova, N. G. Plekhova, E. Drobot, I. Lyapun","doi":"10.36233/0372-9311-2017-5-78-90","DOIUrl":"https://doi.org/10.36233/0372-9311-2017-5-78-90","url":null,"abstract":"","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":"49 1","pages":"78-90"},"PeriodicalIF":0.0,"publicationDate":"2017-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73868971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-10-28DOI: 10.36233/0372-9311-2017-5-70-77
Е. В. Анганова, А. В. Ветохина, Л. А. Распопина, Е. Л. Кичигина, Е. Д. Савилов
Klebsiella pneumoniae microorganisms belong to a group of the most prevalent clinically significant pathogens with a high level of antibacterial resistance (ESKAPE). The speed of formation of antibiotics-resistance by K. pneumoniae strains has sharply increased and reached pandemic scale. One of the main clinically significant mechanisms of antimicrobial resistance is (3-lactamase production, the groups being active depending on the region, country' and hospital. Currently, a significant part of nosocomial K. pneumoniae is resistant to penicillins, 11I-IV generation cephalosporins. The growth of resistance of klebsiellae to carbapenems is a serious threat to the healthcare system. First ofall, KPC-, OXA-, NDM-, VIM-, IMP-producing Widespread of carbapenem-resistant klebsiellae gives evidence on the necessity of international collaboration within the framework of antibiotics resistance control. An increase of frequency of obtained resistance of K. pneumoniae to non-(3-lactame antibiotics (fluoroquinolones, aminoglycosides) is noted. Isolates of K. pneumoniae resistant to tygecyclin, colistin are registered. In general, the problem of antibiotics resistance of causative agents of human diseases including K. pneumoniae continues to intensify. This is a serious threat to world public health that requires action in all sectors of the state.
{"title":"СОСТОЯНИЕ АНТИБИОТИКОРЕЗИСТЕНТНОСТИ KLEBSIELLA PNEUMONIAE","authors":"Е. В. Анганова, А. В. Ветохина, Л. А. Распопина, Е. Л. Кичигина, Е. Д. Савилов","doi":"10.36233/0372-9311-2017-5-70-77","DOIUrl":"https://doi.org/10.36233/0372-9311-2017-5-70-77","url":null,"abstract":"Klebsiella pneumoniae microorganisms belong to a group of the most prevalent clinically significant pathogens with a high level of antibacterial resistance (ESKAPE). The speed of formation of antibiotics-resistance by K. pneumoniae strains has sharply increased and reached pandemic scale. One of the main clinically significant mechanisms of antimicrobial resistance is (3-lactamase production, the groups being active depending on the region, country' and hospital. Currently, a significant part of nosocomial K. pneumoniae is resistant to penicillins, 11I-IV generation cephalosporins. The growth of resistance of klebsiellae to carbapenems is a serious threat to the healthcare system. First ofall, KPC-, OXA-, NDM-, VIM-, IMP-producing Widespread of carbapenem-resistant klebsiellae gives evidence on the necessity of international collaboration within the framework of antibiotics resistance control. An increase of frequency of obtained resistance of K. pneumoniae to non-(3-lactame antibiotics (fluoroquinolones, aminoglycosides) is noted. Isolates of K. pneumoniae resistant to tygecyclin, colistin are registered. In general, the problem of antibiotics resistance of causative agents of human diseases including K. pneumoniae continues to intensify. This is a serious threat to world public health that requires action in all sectors of the state.","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":"20 1","pages":"70-77"},"PeriodicalIF":0.0,"publicationDate":"2017-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89268605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-10-28DOI: 10.36233/0372-9311-2017-5-17-23
N. F. Vasilenko, O. V. Maletskaya, E. A. Manin, D. A. Prislegina, A. S. Volynkina, Ya. V. Lisitskaya, L. I. Shaposhnikova, T. V. Taran, A. Kulichenko
Aim.The analysis of causes of deterioration epidemiological situation on Crimean-Congo haemorrhagic fever (CCHF) in the Russian Federation in 2016. Materials and methods. Data of statistical documentation of Departments of Rospotrebnadzor, Centers of Hygiene and Epidemiology in subjects of the Russian Federation, Plague Control Research Institutions and Anti-Plague stations from 8 subjects of the Southern and 7 subjects of the North-Caucasian Federal Districts were used. The findings have been treated using by Excel program. Results. In 2016, epidemiological manifestations of CCHF are identified on the territory of 6 subjects of the south of Russia. An increase in the incidence of CC H F occurred in the Stavropol Territory, the Republic of Kalmykia, Astrakhan and Volgograd Regions. The main cause of the deterioration epidemiological situation on CCHF was increasing of the number and the period of activity of ticks marginatum, in the epidemic season as a result of favorable climatic conditions; incorrect dates of the realization and inadequacy of the acaricide treatments livestock and natural biotopes. Conclusion. For the improvement epidemiological situation on CCHF timely realization of the acaricide treatments in the early spring (March-April) livestock and natural biotopes using effective insecticides and with its further entomological control it is necessary.
{"title":"ПРИЧИНЫ ОБОСТРЕНИЯ ЭПИДЕМИОЛОГИЧЕСКОЙ ОБСТАНОВКИ ПО КРЫМСКОЙ ГЕМОРРАГИЧЕСКОЙ ЛИХОРАДКЕ В РОССИЙСКОЙ ФЕДЕРАЦИИ В 2016 ГОДУ","authors":"N. F. Vasilenko, O. V. Maletskaya, E. A. Manin, D. A. Prislegina, A. S. Volynkina, Ya. V. Lisitskaya, L. I. Shaposhnikova, T. V. Taran, A. Kulichenko","doi":"10.36233/0372-9311-2017-5-17-23","DOIUrl":"https://doi.org/10.36233/0372-9311-2017-5-17-23","url":null,"abstract":"Aim.The analysis of causes of deterioration epidemiological situation on Crimean-Congo haemorrhagic fever (CCHF) in the Russian Federation in 2016. Materials and methods. Data of statistical documentation of Departments of Rospotrebnadzor, Centers of Hygiene and Epidemiology in subjects of the Russian Federation, Plague Control Research Institutions and Anti-Plague stations from 8 subjects of the Southern and 7 subjects of the North-Caucasian Federal Districts were used. The findings have been treated using by Excel program. Results. In 2016, epidemiological manifestations of CCHF are identified on the territory of 6 subjects of the south of Russia. An increase in the incidence of CC H F occurred in the Stavropol Territory, the Republic of Kalmykia, Astrakhan and Volgograd Regions. The main cause of the deterioration epidemiological situation on CCHF was increasing of the number and the period of activity of ticks marginatum, in the epidemic season as a result of favorable climatic conditions; incorrect dates of the realization and inadequacy of the acaricide treatments livestock and natural biotopes. Conclusion. For the improvement epidemiological situation on CCHF timely realization of the acaricide treatments in the early spring (March-April) livestock and natural biotopes using effective insecticides and with its further entomological control it is necessary.","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":"3 1","pages":"17-23"},"PeriodicalIF":0.0,"publicationDate":"2017-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79135550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-10-28DOI: 10.36233/0372-9311-2017-5-64-70
T. Y. Zagoskina, E. Markov, E. Chaporgina, Yu. O. Popova, T. M. Dolgova, O. Gavrilova, T. S. Taikova, A. V. Nikitina, Pomelova Vg, Osin Ns, S. V. Balakhonov
{"title":"COMPARATIVE ANALYSIS OF EFFECTIVENESS OF SOLID-PHASE METHODS OF IMMUNE DETECTION OF BOTULINIC TOXIN IN BLOOD SERA OF A PATIENT WITH BOTULISM DIAGNOSIS","authors":"T. Y. Zagoskina, E. Markov, E. Chaporgina, Yu. O. Popova, T. M. Dolgova, O. Gavrilova, T. S. Taikova, A. V. Nikitina, Pomelova Vg, Osin Ns, S. V. Balakhonov","doi":"10.36233/0372-9311-2017-5-64-70","DOIUrl":"https://doi.org/10.36233/0372-9311-2017-5-64-70","url":null,"abstract":"","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":"28 1","pages":"64-70"},"PeriodicalIF":0.0,"publicationDate":"2017-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79338806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-08-28DOI: 10.36233/0372-9311-2017-4-35-41
M. L. Ledeneva, A. Vodopianov, G. A. Tkachenko, S. Vodopianov, S. Savchenko, I. Shpak
Aim. Search for potential INDEL-markers in genomes of Burkholderia pseudomallei strains, as well as evaluation of the possibility of their use for intra-species genotyping. Materials and methods. Full-genome sequences of 25 В. pseudomallei strains with known geographic regions of isolation from GenBank database were studied. INDEL-marker search was carried out using authors’ program Gene Expert. Cluster analysis was carried out using genetic distance by R. Sokal and C. Michener and neighbor joining method. Results. 11 INDEL-markers were detected that allowed to split the studied strains into 13 genotypes. Spectrum of INDEL-patterns characteristic for Australian strains was determined. A possibility of existence of certain phylogeographic patterns of Thailand isolates is shown. Conclusion. A possibility of INDEL-markers to differentiate isolates of B. pseudomallei into 2 geographic populations (Australian and South-eastern Asia origin) that allows to determine the source of melioidosis outbreak and transmission routes of the pathogen is detected.
{"title":"DETECTION OF INDEL-MARKERS IN GENOMES OF BURKHOLDERIA PSEUDOMALLEI STRAINS FOR INTRA-SPECIES GENOTYPING","authors":"M. L. Ledeneva, A. Vodopianov, G. A. Tkachenko, S. Vodopianov, S. Savchenko, I. Shpak","doi":"10.36233/0372-9311-2017-4-35-41","DOIUrl":"https://doi.org/10.36233/0372-9311-2017-4-35-41","url":null,"abstract":"Aim. Search for potential INDEL-markers in genomes of Burkholderia pseudomallei strains, as well as evaluation of the possibility of their use for intra-species genotyping. Materials and methods. Full-genome sequences of 25 В. pseudomallei strains with known geographic regions of isolation from GenBank database were studied. INDEL-marker search was carried out using authors’ program Gene Expert. Cluster analysis was carried out using genetic distance by R. Sokal and C. Michener and neighbor joining method. Results. 11 INDEL-markers were detected that allowed to split the studied strains into 13 genotypes. Spectrum of INDEL-patterns characteristic for Australian strains was determined. A possibility of existence of certain phylogeographic patterns of Thailand isolates is shown. Conclusion. A possibility of INDEL-markers to differentiate isolates of B. pseudomallei into 2 geographic populations (Australian and South-eastern Asia origin) that allows to determine the source of melioidosis outbreak and transmission routes of the pathogen is detected.","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":"181 1","pages":"35-41"},"PeriodicalIF":0.0,"publicationDate":"2017-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78163102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-08-28DOI: 10.36233/0372-9311-2017-4-9-17
D. V. Ulshina, D. Kovalev, D. G. Ponomarenko, D. V. Rusanova, N. M. Shvetsova, T. Taran, I. Kuznetsova, A. M. Zhirov, A. Khachaturova, I. Borzdova, A. Kulichenko
{"title":"APPLICATION OF TIME-OF-FLIGHT MASS-SPECTROMETRY FOR DETECTION OF CAUSATIVE AGENT OF BRUCELLOSIS IN BLOOD SAMPLES IN EXPERIMENT","authors":"D. V. Ulshina, D. Kovalev, D. G. Ponomarenko, D. V. Rusanova, N. M. Shvetsova, T. Taran, I. Kuznetsova, A. M. Zhirov, A. Khachaturova, I. Borzdova, A. Kulichenko","doi":"10.36233/0372-9311-2017-4-9-17","DOIUrl":"https://doi.org/10.36233/0372-9311-2017-4-9-17","url":null,"abstract":"","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":"17 1","pages":"9-17"},"PeriodicalIF":0.0,"publicationDate":"2017-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83455972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-08-28DOI: 10.36233/0372-9311-2017-4-48-54
S. A. Gulyaev, I. A. Potemkin, V. S. Kichatova, A. A. Karlsen, O. V. Isaeva, T. V. Guiyaeva, M. A. Vannus, I. V. Gordeichuk, K. K. Kyuregyan, M. I. Mikhailov
Aim. Experimental reproduction of HEV-infection in mini-pigs and comparative analysis of virological and immunological characteristics of experimental infection. Materials and methods. Wiesenauer minipigs (2 females and 4 males, age 50 - 60 days, weight 5-10 kg) were infected by HEV genotype 3 strain isolated from swine feces in Belgorod region in 2013. The virus was administered as a 10% clarified feces extract (800 pi). Blood (weekly) and feces (daily) were sampled from the animals for 49 days. Anti-HEV IgG were determined in sera samples using DS- ElA-ANTI-HEV-G (Diagnostic Systems) system, HEV RNA in samples of feces extracts and blood sera - RT-PCR. Results. Wiesenauer minipigs were sensitive to HEV genotype 3 infection, infection developed in all the animals after administration of the virus, that was accompanied by the presence of HEV RNA in feces for 2 weeks and seroconversion by anti-HEV. Comparative analysis of virological characteristics of experimental H EV-infection in mini- and standard pigs has shown, that the duration of the infection in mini-pigs is shorter, and seroconversion by anti-HEV occurs earlier. Conclusion. Sensitivity to HEV genotype 3 and lower weight make mini-pigs a comfortable alternative to standard swine and primates for modelling HEV infection in vivo.
{"title":"МОДЕЛИРОВАНИЕ ВИРУСНОГО ГЕПАТИТА Е НА КАРЛИКОВЫХ ДОМАШНИХ СВИНЬЯХ","authors":"S. A. Gulyaev, I. A. Potemkin, V. S. Kichatova, A. A. Karlsen, O. V. Isaeva, T. V. Guiyaeva, M. A. Vannus, I. V. Gordeichuk, K. K. Kyuregyan, M. I. Mikhailov","doi":"10.36233/0372-9311-2017-4-48-54","DOIUrl":"https://doi.org/10.36233/0372-9311-2017-4-48-54","url":null,"abstract":"Aim. Experimental reproduction of HEV-infection in mini-pigs and comparative analysis of virological and immunological characteristics of experimental infection. Materials and methods. Wiesenauer minipigs (2 females and 4 males, age 50 - 60 days, weight 5-10 kg) were infected by HEV genotype 3 strain isolated from swine feces in Belgorod region in 2013. The virus was administered as a 10% clarified feces extract (800 pi). Blood (weekly) and feces (daily) were sampled from the animals for 49 days. Anti-HEV IgG were determined in sera samples using DS- ElA-ANTI-HEV-G (Diagnostic Systems) system, HEV RNA in samples of feces extracts and blood sera - RT-PCR. Results. Wiesenauer minipigs were sensitive to HEV genotype 3 infection, infection developed in all the animals after administration of the virus, that was accompanied by the presence of HEV RNA in feces for 2 weeks and seroconversion by anti-HEV. Comparative analysis of virological characteristics of experimental H EV-infection in mini- and standard pigs has shown, that the duration of the infection in mini-pigs is shorter, and seroconversion by anti-HEV occurs earlier. Conclusion. Sensitivity to HEV genotype 3 and lower weight make mini-pigs a comfortable alternative to standard swine and primates for modelling HEV infection in vivo.","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":"38 1","pages":"48-54"},"PeriodicalIF":0.0,"publicationDate":"2017-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91333026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-08-28DOI: 10.36233/0372-9311-2017-4-91-100
M. Boichenko, V. Zverev, E. Volchkova
Diseases caused by Salmonella enterica species bacteria remain a healthcare challenge. Salmonella enterica species is divided into typhoid serovars that cause systemic infection and non typhoid serovars that most frequently have a course of gastroenteritis with a development of inflam matory diarrhea. Both types of salmonella are opportunistic intracellular parasites able to invade and reproduce in both professional and non-professional phagocytes, e.g. M-cells and enterocytes. Invasion of cells and reproduction in them relates to functioning of salmonella pathogenicity island genes that determined synthesis of the third type of secretory system (T3SS). Contrary to the salmonella typhoid group serovars, non-typhoid serovars cause a development of inflammatory diarrhea, and effector molecules of T3SS as well as innate immunity components take part in it.
{"title":"INTERACTION OF SALMONELLA WITH HOST ORGANISM","authors":"M. Boichenko, V. Zverev, E. Volchkova","doi":"10.36233/0372-9311-2017-4-91-100","DOIUrl":"https://doi.org/10.36233/0372-9311-2017-4-91-100","url":null,"abstract":"Diseases caused by Salmonella enterica species bacteria remain a healthcare challenge. Salmonella enterica species is divided into typhoid serovars that cause systemic infection and non typhoid serovars that most frequently have a course of gastroenteritis with a development of inflam matory diarrhea. Both types of salmonella are opportunistic intracellular parasites able to invade and reproduce in both professional and non-professional phagocytes, e.g. M-cells and enterocytes. Invasion of cells and reproduction in them relates to functioning of salmonella pathogenicity island genes that determined synthesis of the third type of secretory system (T3SS). Contrary to the salmonella typhoid group serovars, non-typhoid serovars cause a development of inflammatory diarrhea, and effector molecules of T3SS as well as innate immunity components take part in it.","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":"38 1","pages":"91-100"},"PeriodicalIF":0.0,"publicationDate":"2017-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81348672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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