Pub Date : 2017-12-28DOI: 10.36233/0372-9311-2017-6-95-105
S. B. Yatsyshina
This review presents the actual data about structure, genetic diversity and pathogenicity factors of human respiratory syncytial virus (hRSv) and human metapneumovirus - which are the members of new Pneumoviridae family, according to updated taxonomy accepted by the International Committee on Taxonomy of Viruses (ICTV) in 2016. The results of own epidemiological and clinical studies are presented in comparison with literature data. Cyclic recurrence of hRSv circulation was revealed. The clinical and epidemiological characteristics of hRSv and hMpv infections were compared. The leading role of the hRSv in ARVI in children and the importance of hMpv as an etiological agent of respiratory infection in adults are shown. Significance of both viruses as the agents of the lower respiratory tract infections is emphasized. The factors contributing to the prevalence of the hRSv in children with ARI are analyzed. Prevention measures for these infections are considered.
{"title":"ПНЕВМОВИРУСЫ В ИНФЕКЦИОННОЙ ПАТОЛОГИИ ЧЕЛОВЕКА","authors":"S. B. Yatsyshina","doi":"10.36233/0372-9311-2017-6-95-105","DOIUrl":"https://doi.org/10.36233/0372-9311-2017-6-95-105","url":null,"abstract":"This review presents the actual data about structure, genetic diversity and pathogenicity factors of human respiratory syncytial virus (hRSv) and human metapneumovirus - which are the members of new Pneumoviridae family, according to updated taxonomy accepted by the International Committee on Taxonomy of Viruses (ICTV) in 2016. The results of own epidemiological and clinical studies are presented in comparison with literature data. Cyclic recurrence of hRSv circulation was revealed. The clinical and epidemiological characteristics of hRSv and hMpv infections were compared. The leading role of the hRSv in ARVI in children and the importance of hMpv as an etiological agent of respiratory infection in adults are shown. Significance of both viruses as the agents of the lower respiratory tract infections is emphasized. The factors contributing to the prevalence of the hRSv in children with ARI are analyzed. Prevention measures for these infections are considered.","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":"13 1","pages":"95-105"},"PeriodicalIF":0.0,"publicationDate":"2017-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90465143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-12-28DOI: 10.36233/0372-9311-2017-6-32-37
V. Kovalev, N. Filatov, M. Loktionova, T. G. Frolovskaya, A. Linok, G. A. Zhukova, A. A. Goliusov
Aim. Norovirus infection (NVI) epidemiological characteristics evaluation in Moscow at municipal and district levels. Materials and methods. A retrospective analysis of NVI incidence rate was conducted according to the 2009 - 2016 state statistical reporting forms data. Results. In 2009 - 2016 NVI incidence rate has a trend to increase in Moscow, either at municipal and district levels. NVI cases were registered among children mostly (78% in the SEAD of Moscow, 80% in Moscow and 81% in Russia), at the same time adults proportion of NVI incidence rate increased significantly (up to 42,7% in Moscow and 13,9% in the Russian Federation) by 2016. The morbidity dynamics in different child population age groups was effected by the outbreaks cases. Totally in 2011 - 2016 33 outbreaks were registered in Moscow (24,3% from all acute diarrheal infection outbreaks in Moscow for the same period), where 64,3% with nonpercutaneous channel of infection and 35,7% alimentary. Outbreaks were more often recorded in children’s organized collectivity (69,7%). Conclusion. Nowadays in Moscow there is an NVI incidence increase trend is evidenced, with an increase of the adult population proportion in morbidity structure. At this stage, main contribution to group morbidity was made by children 7 - 14 and 15 - 17 years old. One of the reasons for the NVI incidence rate growth in Moscow (2016) could possibly be the lack of effective prevention measures. Actually, specific NVI diagnostics provided only in outbreaks, due to the lack of routine diagnostic tools. The main reason for the NVI group morbidity effective disease area formation is still sanitary and anti-epidemic regime violation at sites and facilities.
{"title":"MODERN EPIDEMIOLOGICAL CHARACTERISTICS OF NOROVIRUS INFECTION IN MOSCOW","authors":"V. Kovalev, N. Filatov, M. Loktionova, T. G. Frolovskaya, A. Linok, G. A. Zhukova, A. A. Goliusov","doi":"10.36233/0372-9311-2017-6-32-37","DOIUrl":"https://doi.org/10.36233/0372-9311-2017-6-32-37","url":null,"abstract":"Aim. Norovirus infection (NVI) epidemiological characteristics evaluation in Moscow at municipal and district levels. Materials and methods. A retrospective analysis of NVI incidence rate was conducted according to the 2009 - 2016 state statistical reporting forms data. Results. In 2009 - 2016 NVI incidence rate has a trend to increase in Moscow, either at municipal and district levels. NVI cases were registered among children mostly (78% in the SEAD of Moscow, 80% in Moscow and 81% in Russia), at the same time adults proportion of NVI incidence rate increased significantly (up to 42,7% in Moscow and 13,9% in the Russian Federation) by 2016. The morbidity dynamics in different child population age groups was effected by the outbreaks cases. Totally in 2011 - 2016 33 outbreaks were registered in Moscow (24,3% from all acute diarrheal infection outbreaks in Moscow for the same period), where 64,3% with nonpercutaneous channel of infection and 35,7% alimentary. Outbreaks were more often recorded in children’s organized collectivity (69,7%). Conclusion. Nowadays in Moscow there is an NVI incidence increase trend is evidenced, with an increase of the adult population proportion in morbidity structure. At this stage, main contribution to group morbidity was made by children 7 - 14 and 15 - 17 years old. One of the reasons for the NVI incidence rate growth in Moscow (2016) could possibly be the lack of effective prevention measures. Actually, specific NVI diagnostics provided only in outbreaks, due to the lack of routine diagnostic tools. The main reason for the NVI group morbidity effective disease area formation is still sanitary and anti-epidemic regime violation at sites and facilities.","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":"2 1","pages":"32-37"},"PeriodicalIF":0.0,"publicationDate":"2017-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85196175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-12-28DOI: 10.36233/0372-9311-2017-6-72-80
G. I. Alatortseva, Sidorov Av, L. Nesterenko, L. N. Luhverchik, V. Dotsenko, I. I. Amiantova, V. Y. Kabargina, A. V. Milovanova, D. S. Vorobev, Yu. I. Ammur, V. M. Blinov, A. Z. Nurmatov, Z. S. Nurmatov, D. A. Baiyzbekova, O. T. Kasymov, K. K. Kyuregyan, M. I. Mikhailov, S. V. Zhavoronok, Zverev Vv
Aim. The development of the hepatitis E virus (HEV) genotype 1 recombinant capsid protein. Materials and methods. Escherichia coli strains, plasmid vectors, serological and clinical samples, ELISA reagent kits, molecular biological, bioinformatic, biotechnological, biochemical and serological methods. Results. Using HEV genotype 1 DNA copy of subgenomic virus RNA we made E.coli strains producing recombinabt capsid protein, containing C-terminal fragment of ORF2 protein fused to E.coli beta-galactosidase. Recombinant protein ORF2 had been isolated from the inclusion bodies of the E.coli biomass and purified by size exclusion chromatography. By Western blotting it had been shown specific interaction of the recombinant polypeptide with anti-HEV IgG from pool of positive sera. Antigenic specificity of the recombinant polypeptide had been confirmed by enzyme-linked immunosorbent assay with sera of hepatitis E patients and reference groups: healthy donors, patients with hepatitis А, В, C, infectious mononucleosis and cytomegalovirus infection, HIV-infected patients. Conclusion. HEV genotype 1 ORF2 recombinant antigen had been developed, and its possible use in diagnostic tests had been experimentally shown.
{"title":"ПОЛУЧЕНИЕ РЕКОМБИНАНТНОГО АНАЛОГА КАПСИДНОГО БЕЛКА ВИРУСА ГЕПАТИТА Е 1 ГЕНОТИПА: КЛОНИРОВАНИЕ, ЭКСПРЕССИЯ, ОЧИСТКА, ОЦЕНКА АНТИГЕННЫХ СВОЙСТВ","authors":"G. I. Alatortseva, Sidorov Av, L. Nesterenko, L. N. Luhverchik, V. Dotsenko, I. I. Amiantova, V. Y. Kabargina, A. V. Milovanova, D. S. Vorobev, Yu. I. Ammur, V. M. Blinov, A. Z. Nurmatov, Z. S. Nurmatov, D. A. Baiyzbekova, O. T. Kasymov, K. K. Kyuregyan, M. I. Mikhailov, S. V. Zhavoronok, Zverev Vv","doi":"10.36233/0372-9311-2017-6-72-80","DOIUrl":"https://doi.org/10.36233/0372-9311-2017-6-72-80","url":null,"abstract":"Aim. The development of the hepatitis E virus (HEV) genotype 1 recombinant capsid protein. Materials and methods. Escherichia coli strains, plasmid vectors, serological and clinical samples, ELISA reagent kits, molecular biological, bioinformatic, biotechnological, biochemical and serological methods. Results. Using HEV genotype 1 DNA copy of subgenomic virus RNA we made E.coli strains producing recombinabt capsid protein, containing C-terminal fragment of ORF2 protein fused to E.coli beta-galactosidase. Recombinant protein ORF2 had been isolated from the inclusion bodies of the E.coli biomass and purified by size exclusion chromatography. By Western blotting it had been shown specific interaction of the recombinant polypeptide with anti-HEV IgG from pool of positive sera. Antigenic specificity of the recombinant polypeptide had been confirmed by enzyme-linked immunosorbent assay with sera of hepatitis E patients and reference groups: healthy donors, patients with hepatitis А, В, C, infectious mononucleosis and cytomegalovirus infection, HIV-infected patients. Conclusion. HEV genotype 1 ORF2 recombinant antigen had been developed, and its possible use in diagnostic tests had been experimentally shown.","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":"46 1","pages":"72-80"},"PeriodicalIF":0.0,"publicationDate":"2017-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84820248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-12-28DOI: 10.36233/0372-9311-2017-6-25-31
A. A. Asratyan, E. G. Simonova, S. Kazaryan, O. A. Orlova, K. V. Ilenkina, S. R. Raichich, N. B. Sipacheva, N. Karazhas
{"title":"EPSTEIN - BARR VIRAL INFECTION: MODERN SITUATION AND CLINICAL-EPIDEMIOLOGICAL FEATURES AMONG FERTILE WOMEN AND NEWBORNS","authors":"A. A. Asratyan, E. G. Simonova, S. Kazaryan, O. A. Orlova, K. V. Ilenkina, S. R. Raichich, N. B. Sipacheva, N. Karazhas","doi":"10.36233/0372-9311-2017-6-25-31","DOIUrl":"https://doi.org/10.36233/0372-9311-2017-6-25-31","url":null,"abstract":"","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":"16 1","pages":"25-31"},"PeriodicalIF":0.0,"publicationDate":"2017-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73578500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-10-28DOI: 10.36233/0372-9311-2017-5-46-52
N. Novikova, T. A. Sashina, L. Solntsev, Epifanova Nv, A. Y. Kashnikov, L. V. Pogodina, I. Okun, O. N. Knyagina
Aim. Study the manifestations of epidemic process of rotavirus infection in Nizhny Novgorod in pre-vaccination period to evaluate the possible effect on morbidity for the rotavirus vaccine application introduction. Materials and methods. Rotavirus morbidity data were analyzed for the 12-year period (2005 - 2016), as well as its age and season distribution. Rotavirus genotyping was carried out using multiplex PCR and partial sequencing of VP4 and VP7 genes. Results. RVI morbidity in Nizhny Novgorod was shown to be at a moderate level when specific prophylaxis was not applied, multi-year dynamics for morbidity reflects the all-Russian state. 2015 - 2016 were characterized by intensification of the epidemic process in age groups of organized children (3 - 6 and 7 - 14) and adults. Season increase included December-May. seasonal morbidity maximums in different age groups took place during different months. Genetic structure of Nizhny Novgorod population PV-A during this time was presented by 10 types with G9P[8] (44,4%) dominating. Conclusion. Growth of RVI morbidity in Nizhny Novgorod in 2015 - 2016 and changes in age and season manifestations of the infection took place under the condition of change of the dominating genotype PV-A (G4P[8] to G9P[8]).
{"title":"MANIFESTATIONS OF EPIDEMIC PROCESS OF ROTAVIRUS INFECTION IN NIZHNY NOVGOROD IN PRE-VACCINATION PERIOD","authors":"N. Novikova, T. A. Sashina, L. Solntsev, Epifanova Nv, A. Y. Kashnikov, L. V. Pogodina, I. Okun, O. N. Knyagina","doi":"10.36233/0372-9311-2017-5-46-52","DOIUrl":"https://doi.org/10.36233/0372-9311-2017-5-46-52","url":null,"abstract":"Aim. Study the manifestations of epidemic process of rotavirus infection in Nizhny Novgorod in pre-vaccination period to evaluate the possible effect on morbidity for the rotavirus vaccine application introduction. Materials and methods. Rotavirus morbidity data were analyzed for the 12-year period (2005 - 2016), as well as its age and season distribution. Rotavirus genotyping was carried out using multiplex PCR and partial sequencing of VP4 and VP7 genes. Results. RVI morbidity in Nizhny Novgorod was shown to be at a moderate level when specific prophylaxis was not applied, multi-year dynamics for morbidity reflects the all-Russian state. 2015 - 2016 were characterized by intensification of the epidemic process in age groups of organized children (3 - 6 and 7 - 14) and adults. Season increase included December-May. seasonal morbidity maximums in different age groups took place during different months. Genetic structure of Nizhny Novgorod population PV-A during this time was presented by 10 types with G9P[8] (44,4%) dominating. Conclusion. Growth of RVI morbidity in Nizhny Novgorod in 2015 - 2016 and changes in age and season manifestations of the infection took place under the condition of change of the dominating genotype PV-A (G4P[8] to G9P[8]).","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":"22 1","pages":"46-52"},"PeriodicalIF":0.0,"publicationDate":"2017-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86638384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-10-28DOI: 10.36233/0372-9311-2017-5-32-38
A. Kaloshin, A. Soldatenkova, E. M. Zimina, N. Mikhailova
Aim. Obtaining fused recombinant proteins of Pseudomonas aeruginosa that have protective properties against experimental pseudomonas infection. Materials and methods. Fused sequences of P. aeruginosa genes oprF, oprl and deleted form of toxA were cloned in plasmids for the expression in Escherichia coli. The synthesized recombinant proteins were purified in Ni-sepharose columns. Recombinant proteins were administered to mice intraperitonealiy twice with a 2 week interval to evaluate protective properties. Virulent culture of P. aeruginosa strain PA103 was injected into the animals intraperitonealiy 2 weeks after the immunization course as experimental challenge. Results. 3 fused recombinant proteins were produced: 1. OprF-ΔOprl included full sequence of OprF protein and deletion variant of OprI (lacking first 20 amino acids); 2. AOprF-AOprl consisted of C-terminal region (192 - 342 amino acids) OprF and deletion variant of Oprl protein; 3. OprF-aTox-ΔOprI included full sequence of OprF protein, sequence of nontoxic variant of exotoxin A (without 106 C-terminal amino acids) and deletion variant of Oprl protein. Fused recombinant proteins OprF-AOprl and OprF-aTox-ΔOprI at immunization doses of 25 and 50 pg for the first and second protein, respectively, were shown to have the best protective properties. Conclusion. The results obtained open perspectives for further studies to create specific immune biological preparations based on fused recombinant proteins of P. aeruginosa.
{"title":"OBTAINING FUSED RECOMBINANT PROTEINS OprF-ΔOprI, ΔOprF-ΔOprI AND OprF-aTox-ΔOprl OF PSEUDOMONAS AERUGINOSA","authors":"A. Kaloshin, A. Soldatenkova, E. M. Zimina, N. Mikhailova","doi":"10.36233/0372-9311-2017-5-32-38","DOIUrl":"https://doi.org/10.36233/0372-9311-2017-5-32-38","url":null,"abstract":"Aim. Obtaining fused recombinant proteins of Pseudomonas aeruginosa that have protective properties against experimental pseudomonas infection. Materials and methods. Fused sequences of P. aeruginosa genes oprF, oprl and deleted form of toxA were cloned in plasmids for the expression in Escherichia coli. The synthesized recombinant proteins were purified in Ni-sepharose columns. Recombinant proteins were administered to mice intraperitonealiy twice with a 2 week interval to evaluate protective properties. Virulent culture of P. aeruginosa strain PA103 was injected into the animals intraperitonealiy 2 weeks after the immunization course as experimental challenge. Results. 3 fused recombinant proteins were produced: 1. OprF-ΔOprl included full sequence of OprF protein and deletion variant of OprI (lacking first 20 amino acids); 2. AOprF-AOprl consisted of C-terminal region (192 - 342 amino acids) OprF and deletion variant of Oprl protein; 3. OprF-aTox-ΔOprI included full sequence of OprF protein, sequence of nontoxic variant of exotoxin A (without 106 C-terminal amino acids) and deletion variant of Oprl protein. Fused recombinant proteins OprF-AOprl and OprF-aTox-ΔOprI at immunization doses of 25 and 50 pg for the first and second protein, respectively, were shown to have the best protective properties. Conclusion. The results obtained open perspectives for further studies to create specific immune biological preparations based on fused recombinant proteins of P. aeruginosa.","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":"43 1","pages":"32-38"},"PeriodicalIF":0.0,"publicationDate":"2017-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73796924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-10-28DOI: 10.36233/0372-9311-2017-5-3-9
Ю. Ю. Филиппова, Александра Леонидовна Бурмистрова
Aim. Detect connection between cognitive dysfunction in senior people with systemic infection profile and small intestine microbiota structure. Materials and methods. 42 senior individuals were included into the study All the examined have been divided into 2 groups based on the results of psychic status: 20 individuals with moderate cognitive disorders - «active longevity» group and 22 individuals with pronounced cognitive deficiency - «mercy» group. Systemic inflammation status and structure and quantitative composition of microbiota of small intestine was determined in all the senior individuals using gas chromatography mass-spectrometry of microbial markers. Results. Interconnection between systemic level of cytokines and microbiota structure of small intestine in senior people with various cognitive status was determined. Significant correlations between levels of pro-inflammatory cytokine IL-6 in blood plasma and quantity of Propionibacterium jensenii, Moraxella spp., Bacillus cereus and Fusobacterium spp. in small intestine and the degree of cognitive failure were detected for the first time. Conclusion. The parameters obtained could be used as predictive biomarkers of cognitive dysfunction in senior people.
{"title":"КОГНИТИВНАЯ ОСЬ СТАРОСТИ: ВОСПАЛЕНИЕ - МИКРОБИОТА ТОНКОГО КИШЕЧНИКА","authors":"Ю. Ю. Филиппова, Александра Леонидовна Бурмистрова","doi":"10.36233/0372-9311-2017-5-3-9","DOIUrl":"https://doi.org/10.36233/0372-9311-2017-5-3-9","url":null,"abstract":"Aim. Detect connection between cognitive dysfunction in senior people with systemic infection profile and small intestine microbiota structure. Materials and methods. 42 senior individuals were included into the study All the examined have been divided into 2 groups based on the results of psychic status: 20 individuals with moderate cognitive disorders - «active longevity» group and 22 individuals with pronounced cognitive deficiency - «mercy» group. Systemic inflammation status and structure and quantitative composition of microbiota of small intestine was determined in all the senior individuals using gas chromatography mass-spectrometry of microbial markers. Results. Interconnection between systemic level of cytokines and microbiota structure of small intestine in senior people with various cognitive status was determined. Significant correlations between levels of pro-inflammatory cytokine IL-6 in blood plasma and quantity of Propionibacterium jensenii, Moraxella spp., Bacillus cereus and Fusobacterium spp. in small intestine and the degree of cognitive failure were detected for the first time. Conclusion. The parameters obtained could be used as predictive biomarkers of cognitive dysfunction in senior people.","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":"34 1","pages":"3-9"},"PeriodicalIF":0.0,"publicationDate":"2017-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75620891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-10-28DOI: 10.36233/0372-9311-2017-5-53-58
Aleshkin Av, O. Borisova, Gadua Nt, S. Bochkareva, V. A. Chernova, Trebunskikh Ip, B. A. Efimov, L. Kafarskaya, S. Afanasiev, Aleshkin Va, M. Afanasiev, A. Borisova, A. Karaulov
Aim. Evaluate the composition of microorganisms of skin microbiocenosis of axilla in brom-hidrosis patients. Materials and methods. 23 patients were examined (11 - 17 years) under the observation at Pirogov CCDC of the National Medical-Surgery Centre. Identification was carried out using biochemical test-systems BioMerieux VITEK MS MALDI-TOF («bioMerieux», France) and 16SrRNA genesequencing with consequent juxtaposition with EMBL/NCBI. Medium and high degree of skin seeding with microbiota was present in most of the patients with bromhidrosis (52.2 and 43.5%). 137 strains belonging to 5 genera of microorganisms were identified - Corynebacterium, Staphylococcus, Moraxella, Micrococcus, Candida and Bacillus spp. Coiynehacte-rium genus strains (8 species) and Staphylococcus genus (5 species) prevailed in microbiocenosis (89.1%). C. tuberculostearicum strains dominated among Corynebacterium, and S. hominis - Staphylococcus. Conclusion. In most of the cases (82.6%) in patients microbiocenosis of skin of axilla was presented by consortiums of microorganisms with prevalence of Corynebacterium and Staphylococcus microorganisms.
的目标。评价溴化氢患者腋窝皮肤微生物病的微生物组成。材料和方法。23名患者(11 - 17岁)在国家外科医疗中心皮罗戈夫中心接受了检查。使用生化检测系统BioMerieux VITEK MS MALDI-TOF(«BioMerieux»，法国)进行鉴定，并对16SrRNA基因进行测序，随后与EMBL/NCBI并并。腋臭患者皮肤中、高度微生物群播种率分别为52.2%和43.5%。共检出杆状杆菌、葡萄球菌、莫拉菌、微球菌、念珠菌和芽孢杆菌5属137株，其中以coiynehacium属(8种)和葡萄球菌属(5种)为主(89.1%)。硬脂结核杆菌在棒状杆菌和人型葡萄球菌中占主导地位。结论。腋窝皮肤微生物性病变以棒状杆菌和葡萄球菌为主的微生物群为主，占82.6%。
{"title":"MICROBIOCENOSIS OF SKIN IN BROMHIDROSIS PATIENTS","authors":"Aleshkin Av, O. Borisova, Gadua Nt, S. Bochkareva, V. A. Chernova, Trebunskikh Ip, B. A. Efimov, L. Kafarskaya, S. Afanasiev, Aleshkin Va, M. Afanasiev, A. Borisova, A. Karaulov","doi":"10.36233/0372-9311-2017-5-53-58","DOIUrl":"https://doi.org/10.36233/0372-9311-2017-5-53-58","url":null,"abstract":"Aim. Evaluate the composition of microorganisms of skin microbiocenosis of axilla in brom-hidrosis patients. Materials and methods. 23 patients were examined (11 - 17 years) under the observation at Pirogov CCDC of the National Medical-Surgery Centre. Identification was carried out using biochemical test-systems BioMerieux VITEK MS MALDI-TOF («bioMerieux», France) and 16SrRNA genesequencing with consequent juxtaposition with EMBL/NCBI. Medium and high degree of skin seeding with microbiota was present in most of the patients with bromhidrosis (52.2 and 43.5%). 137 strains belonging to 5 genera of microorganisms were identified - Corynebacterium, Staphylococcus, Moraxella, Micrococcus, Candida and Bacillus spp. Coiynehacte-rium genus strains (8 species) and Staphylococcus genus (5 species) prevailed in microbiocenosis (89.1%). C. tuberculostearicum strains dominated among Corynebacterium, and S. hominis - Staphylococcus. Conclusion. In most of the cases (82.6%) in patients microbiocenosis of skin of axilla was presented by consortiums of microorganisms with prevalence of Corynebacterium and Staphylococcus microorganisms.","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":"63 1","pages":"53-58"},"PeriodicalIF":0.0,"publicationDate":"2017-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88049264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-10-28DOI: 10.36233/0372-9311-2017-5-10-17
D. V. Efremenko, O. Maletskaya, V. Orobey, V. Efremenko
Topical issues of ensuring biological safety of mass actions are considered in the article. According to the purpose and content are allocated two interdependent and supplementing each other within the system of supervision and monitoring of biological safety — the measures for prophylaxis of emergency situations and the measures for ensuring readiness for emergency situ ations. Actions of separate functional elements of system of rather accompanying epidemiological risks focused on their decrease and monitoring are analyzed. The attributive base is developed for the principles of ensuring biological safety (rationality, competence, efficiency, flexibility, control lability, preventiveness, coherence) which are considered as determinants of solvable tasks during the periods of preparation and holding mass actions.
{"title":"BIOLOGICAL SAFETY OF MASS ACTIONS: TOPICAL ISSUES AND PRINCIPLES","authors":"D. V. Efremenko, O. Maletskaya, V. Orobey, V. Efremenko","doi":"10.36233/0372-9311-2017-5-10-17","DOIUrl":"https://doi.org/10.36233/0372-9311-2017-5-10-17","url":null,"abstract":"Topical issues of ensuring biological safety of mass actions are considered in the article. According to the purpose and content are allocated two interdependent and supplementing each other within the system of supervision and monitoring of biological safety — the measures for prophylaxis of emergency situations and the measures for ensuring readiness for emergency situ ations. Actions of separate functional elements of system of rather accompanying epidemiological risks focused on their decrease and monitoring are analyzed. The attributive base is developed for the principles of ensuring biological safety (rationality, competence, efficiency, flexibility, control lability, preventiveness, coherence) which are considered as determinants of solvable tasks during the periods of preparation and holding mass actions.","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":"194 1","pages":"10-17"},"PeriodicalIF":0.0,"publicationDate":"2017-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73278463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-10-28DOI: 10.36233/0372-9311-2017-5-24-32
O. Borisova, A. S. Pimenova, A. V. Chaplin, L. Kafarskaya, S. Afanasiev, Aleshkin Va, Aleshkin Av, M. Afanasiev, A. Karaulov
of C. from bacteriological the and sent to the of Epidemiology and Microbiology. Strain isolation was carried out in accordance with MI 4.2.698-98 and 4.2.3065-13. Chromosomal DNA was isolated by standard heating method, as well as using 3 commercial kits. Detection of the amplification results was carried out in horizontal electrophoresis in 1.5% agarose gel. Results. The developed method of gene diagnostics was established to allow detection of DNA of toxi genic C. diphtheriae strains of 2 biovars, as well as DNA of non-toxigenic tox-gene bearing strains (NTTB) of C. diphtheriae mitis biovar with mechanisms of lack of expression of diphtheria toxin gene due to the presence of deletion or mobile genetic IS element in the tox gene. Non-toxigenic tox-gene bearing C. diphtheriae strain with the mechanism of lack of diphtheria toxin gene expres sion due to the presence oftransposon in the tox gene are identified as non-toxigenic. Evaluation of the analytical sensitivity in comparative studies using 3 commercial kits for FNA isolation has shown that sensitivity reached 4.5x10' GE/ml using Ribo-prep kit. High specificity of the developed method is shown, it was evaluated in 18 strains of 16 other members of the Corynebacterium genus and 20 typical strains of microorganisms isolated from oropharynx or causing infections of the respiratory tract. Approbation of the developed method was carried out in model experiments in imitators of clinical samples by infection of single-use sterile dry tampons by consecutive dilutions of the bacterial cultures (with parallel seeding into dense nutrient media) and was 103 GE/ml. Conclusion. The developed method of accelerated gene diagnostics of the diphtheria infection has a high diagnostic effectiveness, specificity and sensitivity, allows to detect 103 — 4.5x10 GE/ml C. diphtheriae in clinical material with simultaneous verification of toxigenic and non-toxigenic strains. fused recombinant proteins of Pseudomonas that infection. P.
{"title":"AN ACCELERATED METHOD OF DIPHTHERIA GENE DIAGNOSTICS BASED ON ISOTHERMAL AMPLIFICATION TO DETECT DNA OF THE CAUSATIVE AGENT","authors":"O. Borisova, A. S. Pimenova, A. V. Chaplin, L. Kafarskaya, S. Afanasiev, Aleshkin Va, Aleshkin Av, M. Afanasiev, A. Karaulov","doi":"10.36233/0372-9311-2017-5-24-32","DOIUrl":"https://doi.org/10.36233/0372-9311-2017-5-24-32","url":null,"abstract":"of C. from bacteriological the and sent to the of Epidemiology and Microbiology. Strain isolation was carried out in accordance with MI 4.2.698-98 and 4.2.3065-13. Chromosomal DNA was isolated by standard heating method, as well as using 3 commercial kits. Detection of the amplification results was carried out in horizontal electrophoresis in 1.5% agarose gel. Results. The developed method of gene diagnostics was established to allow detection of DNA of toxi genic C. diphtheriae strains of 2 biovars, as well as DNA of non-toxigenic tox-gene bearing strains (NTTB) of C. diphtheriae mitis biovar with mechanisms of lack of expression of diphtheria toxin gene due to the presence of deletion or mobile genetic IS element in the tox gene. Non-toxigenic tox-gene bearing C. diphtheriae strain with the mechanism of lack of diphtheria toxin gene expres sion due to the presence oftransposon in the tox gene are identified as non-toxigenic. Evaluation of the analytical sensitivity in comparative studies using 3 commercial kits for FNA isolation has shown that sensitivity reached 4.5x10' GE/ml using Ribo-prep kit. High specificity of the developed method is shown, it was evaluated in 18 strains of 16 other members of the Corynebacterium genus and 20 typical strains of microorganisms isolated from oropharynx or causing infections of the respiratory tract. Approbation of the developed method was carried out in model experiments in imitators of clinical samples by infection of single-use sterile dry tampons by consecutive dilutions of the bacterial cultures (with parallel seeding into dense nutrient media) and was 103 GE/ml. Conclusion. The developed method of accelerated gene diagnostics of the diphtheria infection has a high diagnostic effectiveness, specificity and sensitivity, allows to detect 103 — 4.5x10 GE/ml C. diphtheriae in clinical material with simultaneous verification of toxigenic and non-toxigenic strains. fused recombinant proteins of Pseudomonas that infection. P.","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":"98 1","pages":"24-32"},"PeriodicalIF":0.0,"publicationDate":"2017-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82665891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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