{"title":"Genome-wide assessment and characterization of simple sequence repeats (SSRs) makers for Capoeta aculeata (Valenciennes, 1844) using NGS data","authors":"H. Gandomkar, S. Nazari, P. Hosseini, H. Abdolhay","doi":"10.52547/injoar.2.2.16","DOIUrl":"https://doi.org/10.52547/injoar.2.2.16","url":null,"abstract":"","PeriodicalId":255746,"journal":{"name":"International Journal of Environmental Research and Education","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126073520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Haghighi, M. Bahmani, A. Yousefi Jourdeh, A. Zamini
Super nutrients are nutrient compounds including fat dissolved vitamins, necessary minerals and growth promoters. Present research was carried out with the aim of determining super nutrients effect on some growth and biochemical indices in juvenile Acipenser baerii in International Sturgeon Research Institute during 6 months. Blood samples obtained in days 45 (stage I) and 90 (stage II) and plasma provided. Some plasma biochemical an ions levels were measured in VIRO MED laboratory in Rasht. The results showed that the mean final weight, final biomass, body weight increasing (BWI), specific growth rate (SGR), condition factor condition factor (CF) and daily growth rate (ADG) showed significant difference between control group with other treatments ( p <0.05). Results showed that sodium, potassium and calcium ion levels showed no significant difference in various concentrations of super nutrients during rearing period ( p >0.05). Based on obtained results, super nutrients adding in diet of Acipenser baerii caused to significant difference in growth indices and improved them. Therefore, it recommended using of super nutrients in Siberian sturgeon diet.
{"title":"Effects of super nutrients on some growth and biochemical indices in Siberian sturgeon (Acipenser baerii)","authors":"H. Haghighi, M. Bahmani, A. Yousefi Jourdeh, A. Zamini","doi":"10.52547/injoar.2.2.1","DOIUrl":"https://doi.org/10.52547/injoar.2.2.1","url":null,"abstract":"Super nutrients are nutrient compounds including fat dissolved vitamins, necessary minerals and growth promoters. Present research was carried out with the aim of determining super nutrients effect on some growth and biochemical indices in juvenile Acipenser baerii in International Sturgeon Research Institute during 6 months. Blood samples obtained in days 45 (stage I) and 90 (stage II) and plasma provided. Some plasma biochemical an ions levels were measured in VIRO MED laboratory in Rasht. The results showed that the mean final weight, final biomass, body weight increasing (BWI), specific growth rate (SGR), condition factor condition factor (CF) and daily growth rate (ADG) showed significant difference between control group with other treatments ( p <0.05). Results showed that sodium, potassium and calcium ion levels showed no significant difference in various concentrations of super nutrients during rearing period ( p >0.05). Based on obtained results, super nutrients adding in diet of Acipenser baerii caused to significant difference in growth indices and improved them. Therefore, it recommended using of super nutrients in Siberian sturgeon diet.","PeriodicalId":255746,"journal":{"name":"International Journal of Environmental Research and Education","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114819608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Gandomkar, S. Nazari, M.M. Salahi Ardakani, E. Kazemi
Tetraploidization of rainbow trout ( Oncorhynchus mikiss ) was induced by hyperthermia and designed in three treatments (28ºC for 10, 12 and 14 min), 7 h after fertilization. Immature fishes were anaesthetised using standard method in tank (Table 1). Blood samples were collected by caudal venipuncture using 2 mL syringes fitted with 0.5 x 35 mm (25 gauge) needles (Sima, Iran) pre-dosed with heparin (Caspian Tamin, Iran). Blood samples subjected to various conditions, were analysed by FC. Methodology was adapted from established protocols for human blood FC analysis, but modified as required due to the nucleated nature of fish erythrocytes. Blood sample evaluations by FC were performed in surface-deactivated polypropylene tubes to minimize cell-tube adhesion and associated quantitation error. Five millilitre 12 x 75 mm polypropylene Falcon tubes (Maxwell, Italy) were filled with a 4% solution of bovine serum albumin (BSA; Sigma, USA) in PBS and stored overnight at 4°C (Lecommandeur et al., 1994). Prior to use, the tubes were emptied and centrifuged at 2000 rpm for 5 min. For each treatment, we also measured the size of erythrocytes and genome size. Genome size was positively correlated with erythrocyte nucleus size and chromosome number when using PI as the fluorescent dye. This work provides new knowledge on Oncorhynchus mykiss genetics/genomics, important for future research in basic cellular/molecular mechanisms and for the development of molecular techniques in this species. However, further investigation is required to obtain a high percent tetraploid Rainbow trout population.
以虹鳟鱼(Oncorhynchus mikiss)为研究对象,在受精后7 h,采用28℃高温处理10、12、14 min,诱导虹鳟鱼四倍体分化。未成熟鱼在池中使用标准方法麻醉(表1)。使用2ml注射器(配有0.5 x 35 mm(25号)针头(伊朗Sima),预先注射肝素(伊朗Caspian Tamin),通过尾端静脉穿刺采集血样。不同条件下的血液样本被FC分析。方法改编自人类血液FC分析的既定方案,但由于鱼类红细胞的有核性质而根据需要进行修改。血液样品的FC评估在表面失活的聚丙烯管中进行,以尽量减少细胞-管粘连和相关的定量误差。5毫升12 × 75 mm聚丙烯Falcon管(Maxwell, Italy)中填充4%牛血清白蛋白(BSA;Sigma, USA)在PBS中保存,并在4°C下保存过夜(Lecommandeur et al, 1994)。在使用之前,将试管清空并以2000 rpm离心5分钟。对于每次处理,我们还测量了红细胞的大小和基因组的大小。以PI为荧光染料时,基因组大小与红细胞细胞核大小和染色体数目呈正相关。本研究为今后研究该物种的基本细胞/分子机制和分子技术的发展提供了新的知识。然而,需要进一步的调查来获得高百分比的四倍体虹鳟鱼种群。
{"title":"Comparison of genome size of diploid and tetraploid of rainbow trout (Oncorhynchus mykiss)","authors":"H. Gandomkar, S. Nazari, M.M. Salahi Ardakani, E. Kazemi","doi":"10.52547/injoar.2.2.7","DOIUrl":"https://doi.org/10.52547/injoar.2.2.7","url":null,"abstract":"Tetraploidization of rainbow trout ( Oncorhynchus mikiss ) was induced by hyperthermia and designed in three treatments (28ºC for 10, 12 and 14 min), 7 h after fertilization. Immature fishes were anaesthetised using standard method in tank (Table 1). Blood samples were collected by caudal venipuncture using 2 mL syringes fitted with 0.5 x 35 mm (25 gauge) needles (Sima, Iran) pre-dosed with heparin (Caspian Tamin, Iran). Blood samples subjected to various conditions, were analysed by FC. Methodology was adapted from established protocols for human blood FC analysis, but modified as required due to the nucleated nature of fish erythrocytes. Blood sample evaluations by FC were performed in surface-deactivated polypropylene tubes to minimize cell-tube adhesion and associated quantitation error. Five millilitre 12 x 75 mm polypropylene Falcon tubes (Maxwell, Italy) were filled with a 4% solution of bovine serum albumin (BSA; Sigma, USA) in PBS and stored overnight at 4°C (Lecommandeur et al., 1994). Prior to use, the tubes were emptied and centrifuged at 2000 rpm for 5 min. For each treatment, we also measured the size of erythrocytes and genome size. Genome size was positively correlated with erythrocyte nucleus size and chromosome number when using PI as the fluorescent dye. This work provides new knowledge on Oncorhynchus mykiss genetics/genomics, important for future research in basic cellular/molecular mechanisms and for the development of molecular techniques in this species. However, further investigation is required to obtain a high percent tetraploid Rainbow trout population.","PeriodicalId":255746,"journal":{"name":"International Journal of Environmental Research and Education","volume":"95 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124901198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The study was carried out in the genetics and breeding resaerch canter of yasouj. All eye-stage eggs were used for the experiment using vertical hatching units to complete hatching. A standard commercial granule feed was used in all experimental groups. The experiment consisted of control treatment and one dose of hormone 17α-Methyltestosterone added to the diet at the rate of 3 mg per kg food by dissolving in 200 ml of 95% ethanol per kg feed.The use of oral administration of 17α-methyltestosterone (17MT) at 3 mg 17MT/kg of feed for 70 days following the onset of feeding to sex reverse rainbow trout ( Oncorhynchus mykiss ) to produce XX males is well established. Examination of the results showed that 17α- methyltestosterone was effective in sex riversed of rainbow trout. The results has shown in nearly 100% of the treated animals being males. The GSI index in female and male fish of control group were 0.04±0.1 and 0.07±0.01, respectively (Fig.3) and significantly different ( p ≤5%). But GSI index of 100% sex riversed male fish of MT treated was 0.06±0.01 and hadn,t significantly differene ( p ≥5%) with treated group ( genetically male and female).
本研究在雅苏吉遗传育种研究中心进行。所有眼期卵均采用垂直孵化装置完成孵化。各试验组均饲喂标准商品颗粒饲料。试验采用对照处理,将1剂激素17α-甲基睾酮溶解在200 ml 95%乙醇中,以每kg饲料3 mg的速率添加到饲料中。对性别反转的虹鳟鱼(Oncorhynchus mykiss)进行饲喂后,在70天内口服17α-甲基睾酮(17MT),剂量为3mg 17MT/kg饲料,以产生XX雄性。结果表明,17α-甲基睾酮对虹鳟鱼的性流有效。结果显示,几乎100%接受治疗的动物都是雄性。对照组雌鱼和雄鱼GSI指数分别为0.04±0.1和0.07±0.01(图3),差异有统计学意义(p≤5%)。MT处理后100%性河雄鱼GSI指数为0.06±0.01,与处理组(遗传雌雄)无显著差异(p≥5%)。
{"title":"Investigation of sexual maturity and change in gonadosomatic index of sex riversed rainbow trout (Oncorhynchus mykiss) treated by oral 17 alpha methyl testosterone","authors":"A. Rastiannasab, E. Kazemi","doi":"10.52547/injoar.2.2.11","DOIUrl":"https://doi.org/10.52547/injoar.2.2.11","url":null,"abstract":"The study was carried out in the genetics and breeding resaerch canter of yasouj. All eye-stage eggs were used for the experiment using vertical hatching units to complete hatching. A standard commercial granule feed was used in all experimental groups. The experiment consisted of control treatment and one dose of hormone 17α-Methyltestosterone added to the diet at the rate of 3 mg per kg food by dissolving in 200 ml of 95% ethanol per kg feed.The use of oral administration of 17α-methyltestosterone (17MT) at 3 mg 17MT/kg of feed for 70 days following the onset of feeding to sex reverse rainbow trout ( Oncorhynchus mykiss ) to produce XX males is well established. Examination of the results showed that 17α- methyltestosterone was effective in sex riversed of rainbow trout. The results has shown in nearly 100% of the treated animals being males. The GSI index in female and male fish of control group were 0.04±0.1 and 0.07±0.01, respectively (Fig.3) and significantly different ( p ≤5%). But GSI index of 100% sex riversed male fish of MT treated was 0.06±0.01 and hadn,t significantly differene ( p ≥5%) with treated group ( genetically male and female).","PeriodicalId":255746,"journal":{"name":"International Journal of Environmental Research and Education","volume":"27 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115437111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This present paper made it possible to update the inventory of benthic malacofauna in the soft bottoms of gulf of Oran. Samples of macrobenthic fauna were collected and separated from the Molluscs from other zoological groups. 116 Molluscs inventoried and determined by the species, including 13 orders, 20 families, 24 genuses and 29 species. Bivalvia is represented better than Gastropoda with respectively, 24 and 5 species. The analyzed taxa highlighted the dominant and main species on the bottom of the study sites, including Nuculana commutata , which appeared as the major species, followed by Nucula sulcata with respectively 23,46% and 12,34%. The present inventory indicates that the malacological fauna is less diversified than in the other zones studied of the Algerian coast.
{"title":"Inventory of marine molluscs in Gulf of Oran \u0000(Western Algerian coastline)","authors":"A. Kerfouf, F. Kies, S. Boucetta, F. Denis","doi":"10.52547/injoere.3.1.17","DOIUrl":"https://doi.org/10.52547/injoere.3.1.17","url":null,"abstract":"This present paper made it possible to update the inventory of benthic malacofauna in the soft bottoms of gulf of Oran. Samples of macrobenthic fauna were collected and separated from the Molluscs from other zoological groups. 116 Molluscs inventoried and determined by the species, including 13 orders, 20 families, 24 genuses and 29 species. Bivalvia is represented better than Gastropoda with respectively, 24 and 5 species. The analyzed taxa highlighted the dominant and main species on the bottom of the study sites, including Nuculana commutata , which appeared as the major species, followed by Nucula sulcata with respectively 23,46% and 12,34%. The present inventory indicates that the malacological fauna is less diversified than in the other zones studied of the Algerian coast.","PeriodicalId":255746,"journal":{"name":"International Journal of Environmental Research and Education","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115374643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}