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Genome-wide assessment and characterization of simple sequence repeats (SSRs) makers for Capoeta aculeata (Valenciennes, 1844) using NGS data 利用NGS数据对卡波塔(Capoeta aculeata, valciennes, 1844)的简单序列重复序列(SSRs)制造者进行全基因组评估和表征
Pub Date : 2022-05-01 DOI: 10.52547/injoar.2.2.16
H. Gandomkar, S. Nazari, P. Hosseini, H. Abdolhay
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引用次数: 0
Effects of super nutrients on some growth and biochemical indices in Siberian sturgeon (Acipenser baerii) 超营养物质对中华鲟(Acipenser baerii)生长及生化指标的影响
Pub Date : 2022-05-01 DOI: 10.52547/injoar.2.2.1
H. Haghighi, M. Bahmani, A. Yousefi Jourdeh, A. Zamini
Super nutrients are nutrient compounds including fat dissolved vitamins, necessary minerals and growth promoters. Present research was carried out with the aim of determining super nutrients effect on some growth and biochemical indices in juvenile Acipenser baerii in International Sturgeon Research Institute during 6 months. Blood samples obtained in days 45 (stage I) and 90 (stage II) and plasma provided. Some plasma biochemical an ions levels were measured in VIRO MED laboratory in Rasht. The results showed that the mean final weight, final biomass, body weight increasing (BWI), specific growth rate (SGR), condition factor condition factor (CF) and daily growth rate (ADG) showed significant difference between control group with other treatments ( p <0.05). Results showed that sodium, potassium and calcium ion levels showed no significant difference in various concentrations of super nutrients during rearing period ( p >0.05). Based on obtained results, super nutrients adding in diet of Acipenser baerii caused to significant difference in growth indices and improved them. Therefore, it recommended using of super nutrients in Siberian sturgeon diet.
超级营养素是包括脂溶性维生素、必需矿物质和生长促进剂在内的营养化合物。本研究旨在测定超营养物质对国际鲟鱼研究所巴氏鲟幼鱼6个月期间某些生长和生化指标的影响。第45天(I期)和第90天(II期)采集血样并提供血浆。在拉沙市VIRO MED实验室测定了部分血浆生化和离子水平。结果表明:对照组与其他处理的平均末重、末生物量、增重(BWI)、特定生长率(SGR)、条件因子(CF)和日生长率(ADG)差异显著(p 0.05)。综上所述,饲料中添加超营养物质对巴氏鲟的生长指标有显著影响并有改善作用。因此,建议在中华鲟日粮中添加超营养物质。
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引用次数: 0
Comparison of genome size of diploid and tetraploid of rainbow trout (Oncorhynchus mykiss) 虹鳟二倍体与四倍体基因组大小的比较
Pub Date : 2022-05-01 DOI: 10.52547/injoar.2.2.7
H. Gandomkar, S. Nazari, M.M. Salahi Ardakani, E. Kazemi
Tetraploidization of rainbow trout ( Oncorhynchus mikiss ) was induced by hyperthermia and designed in three treatments (28ºC for 10, 12 and 14 min), 7 h after fertilization. Immature fishes were anaesthetised using standard method in tank (Table 1). Blood samples were collected by caudal venipuncture using 2 mL syringes fitted with 0.5 x 35 mm (25 gauge) needles (Sima, Iran) pre-dosed with heparin (Caspian Tamin, Iran). Blood samples subjected to various conditions, were analysed by FC. Methodology was adapted from established protocols for human blood FC analysis, but modified as required due to the nucleated nature of fish erythrocytes. Blood sample evaluations by FC were performed in surface-deactivated polypropylene tubes to minimize cell-tube adhesion and associated quantitation error. Five millilitre 12 x 75 mm polypropylene Falcon tubes (Maxwell, Italy) were filled with a 4% solution of bovine serum albumin (BSA; Sigma, USA) in PBS and stored overnight at 4°C (Lecommandeur et al., 1994). Prior to use, the tubes were emptied and centrifuged at 2000 rpm for 5 min. For each treatment, we also measured the size of erythrocytes and genome size. Genome size was positively correlated with erythrocyte nucleus size and chromosome number when using PI as the fluorescent dye. This work provides new knowledge on Oncorhynchus mykiss genetics/genomics, important for future research in basic cellular/molecular mechanisms and for the development of molecular techniques in this species. However, further investigation is required to obtain a high percent tetraploid Rainbow trout population.
以虹鳟鱼(Oncorhynchus mikiss)为研究对象,在受精后7 h,采用28℃高温处理10、12、14 min,诱导虹鳟鱼四倍体分化。未成熟鱼在池中使用标准方法麻醉(表1)。使用2ml注射器(配有0.5 x 35 mm(25号)针头(伊朗Sima),预先注射肝素(伊朗Caspian Tamin),通过尾端静脉穿刺采集血样。不同条件下的血液样本被FC分析。方法改编自人类血液FC分析的既定方案,但由于鱼类红细胞的有核性质而根据需要进行修改。血液样品的FC评估在表面失活的聚丙烯管中进行,以尽量减少细胞-管粘连和相关的定量误差。5毫升12 × 75 mm聚丙烯Falcon管(Maxwell, Italy)中填充4%牛血清白蛋白(BSA;Sigma, USA)在PBS中保存,并在4°C下保存过夜(Lecommandeur et al, 1994)。在使用之前,将试管清空并以2000 rpm离心5分钟。对于每次处理,我们还测量了红细胞的大小和基因组的大小。以PI为荧光染料时,基因组大小与红细胞细胞核大小和染色体数目呈正相关。本研究为今后研究该物种的基本细胞/分子机制和分子技术的发展提供了新的知识。然而,需要进一步的调查来获得高百分比的四倍体虹鳟鱼种群。
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引用次数: 0
Investigation of sexual maturity and change in gonadosomatic index of sex riversed rainbow trout (Oncorhynchus mykiss) treated by oral 17 alpha methyl testosterone 口服17 α -甲基睾酮对虹鳟鱼性成熟及性腺指标变化的影响
Pub Date : 2022-05-01 DOI: 10.52547/injoar.2.2.11
A. Rastiannasab, E. Kazemi
The study was carried out in the genetics and breeding resaerch canter of yasouj. All eye-stage eggs were used for the experiment using vertical hatching units to complete hatching. A standard commercial granule feed was used in all experimental groups. The experiment consisted of control treatment and one dose of hormone 17α-Methyltestosterone added to the diet at the rate of 3 mg per kg food by dissolving in 200 ml of 95% ethanol per kg feed.The use of oral administration of 17α-methyltestosterone (17MT) at 3 mg 17MT/kg of feed for 70 days following the onset of feeding to sex reverse rainbow trout ( Oncorhynchus mykiss ) to produce XX males is well established. Examination of the results showed that 17α- methyltestosterone was effective in sex riversed of rainbow trout. The results has shown in nearly 100% of the treated animals being males. The GSI index in female and male fish of control group were 0.04±0.1 and 0.07±0.01, respectively (Fig.3) and significantly different ( p ≤5%). But GSI index of 100% sex riversed male fish of MT treated was 0.06±0.01 and hadn,t significantly differene ( p ≥5%) with treated group ( genetically male and female).
本研究在雅苏吉遗传育种研究中心进行。所有眼期卵均采用垂直孵化装置完成孵化。各试验组均饲喂标准商品颗粒饲料。试验采用对照处理,将1剂激素17α-甲基睾酮溶解在200 ml 95%乙醇中,以每kg饲料3 mg的速率添加到饲料中。对性别反转的虹鳟鱼(Oncorhynchus mykiss)进行饲喂后,在70天内口服17α-甲基睾酮(17MT),剂量为3mg 17MT/kg饲料,以产生XX雄性。结果表明,17α-甲基睾酮对虹鳟鱼的性流有效。结果显示,几乎100%接受治疗的动物都是雄性。对照组雌鱼和雄鱼GSI指数分别为0.04±0.1和0.07±0.01(图3),差异有统计学意义(p≤5%)。MT处理后100%性河雄鱼GSI指数为0.06±0.01,与处理组(遗传雌雄)无显著差异(p≥5%)。
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引用次数: 1
Inventory of marine molluscs in Gulf of Oran (Western Algerian coastline) 奥兰湾(阿尔及利亚西部海岸线)海洋软体动物清查
Pub Date : 1900-01-01 DOI: 10.52547/injoere.3.1.17
A. Kerfouf, F. Kies, S. Boucetta, F. Denis
This present paper made it possible to update the inventory of benthic malacofauna in the soft bottoms of gulf of Oran. Samples of macrobenthic fauna were collected and separated from the Molluscs from other zoological groups. 116 Molluscs inventoried and determined by the species, including 13 orders, 20 families, 24 genuses and 29 species. Bivalvia is represented better than Gastropoda with respectively, 24 and 5 species. The analyzed taxa highlighted the dominant and main species on the bottom of the study sites, including Nuculana commutata , which appeared as the major species, followed by Nucula sulcata with respectively 23,46% and 12,34%. The present inventory indicates that the malacological fauna is less diversified than in the other zones studied of the Algerian coast.
本文的研究为更新奥兰湾软底底栖藻的清查提供了可能。收集了大型底栖动物的标本,并与其他动物类群的软体动物进行了分离。已调查确定的软体动物116种,包括13目20科24属29种。双壳纲比腹足纲的代表性更强,分别有24种和5种。在研究点底部的分类群中,优势种和主要种居多,其中对向Nuculana commutata占主要种,其次是Nucula sulcata,分别占23.46%和12.34%。目前的清查表明,与阿尔及利亚海岸研究的其他地区相比,软体动物群的多样性较低。
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引用次数: 0
Spatial and temporal distribution pattern and biomass trend of flathead slipper lobster, Thenus orientalis (Lund, 1793) from Gulf of Oman 阿曼湾石首虾(Thenus orientalis, Lund, 1793)时空分布格局及生物量趋势
Pub Date : 1900-01-01 DOI: 10.52547/injoere.3.1.27
Majid Mirzaei, A. Ajdari, S. P. Woo
The aim of the present study was to investigate the amount of biomass and Catch Per Unit Area (CPUA) as well as to determine the distribution pattern of flathead slipper lobster, Thenus orientalis as one of the most important and commercial aquatic species in the north coast of the Gulf of Oman. There was an annual monitoring survey during 2009-2019 by using R/V ʻ Ferdows-1 ʼ covering the depths10-100 m which equipped with bottom trawl net. The highest CPUA was recorded in eastern and western region of the study area (45.54 kg/nm 2 and 25.40 kg/nm 2 ), although density of species was lower in the central area (Stratum B, C, D). The highest biomass (22.7 tons) and CPUA (52.13 kg/nm 2 ) was found in 10-20 m and 20–30 m depth layer, respectively. The comparison of mean CPUA and biomass in different depth-layers and stratum revealed that the mean CPUA and biomass have a descending trend with increasing of depth. Results of this study showed that the abundance of this species dramatically decreased over past decade to about 13.3 kg/nm 2 in 2019. Therefore, it is necessary to implement a regulatory mechanism to manage the stocks of this commercially valuable species, such as closed fishing season during the breeding and reproduction period, only permitted to use traps for harvesting of lobster, prohibited to take egg-bearing females or juvenile lobsters.
本研究的目的是调查阿曼湾北海岸最重要的商业水生物种之一的平头拖鞋龙虾(Thenus orientalis)的生物量和单位面积渔获量(CPUA),并确定其分布格局。在2009-2019年期间,利用R/V h . Ferdows-1进行了年度监测调查,覆盖深度为10-100米,配备了底拖网。中部地区(B、C、D层)的物种密度较低,东部和西部地区的生物量最高,分别为45.54 kg/nm 2和25.40 kg/nm 2, 10 ~ 20 m和20 ~ 30 m层的生物量最高,分别为22.7 t和52.13 kg/nm 2。不同深度层和地层的平均CPUA和生物量比较表明,随着深度的增加,平均CPUA和生物量呈下降趋势。本研究结果表明,该物种的丰度在过去十年中急剧下降,到2019年约为13.3 kg/nm 2。因此,有必要实施一种监管机制来管理这一具有商业价值的物种的种群,例如在繁殖和繁殖期间的闭渔季节,只允许使用陷阱捕捞龙虾,禁止捕捞产卵的雌性或幼年龙虾。
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引用次数: 0
Abiotic stresses and vegetable production in the era of climate change: A review 气候变化时代非生物胁迫与蔬菜生产研究进展
Pub Date : 1900-01-01 DOI: 10.52547/injoere.3.1.37
M. Naeem, Z. Selamoğlu
Global warming has been recognised as a critical hazard to natural ecosystem existence. Changing climate is a continuous, multidimensional process of changes in climatic conditions that affect the abiotic and biotic aspects. This causes changes in climatic circumstances including extreme heat, rainfall amount, and temperatures, which leads to an increase in novel parasites, vegetation, and diseases, while vegetables crops must employ specific methods such as physiological, biochemcial and molecualar process to ensure optimal development during stress situations. The synthesis of hormones (phytohormones) and osmolytes is one of these processes. Plant hormones are natural materials which influence the physiological processes of plants such as growth, development, and cell differentiation. Under normal and stressful situations, plant hormones regulate essential physiological activities. Phytohormones, which come in a variety of forms, serve a significant role in plants, either on their own or in concert with one another. The type and concentration of such substances in plants have a significant impact on plant activities and functions. The current study describes phytohormones' possible involvement in fostering plant growth and development during stressful conditions.
全球变暖已被认为是对自然生态系统存在的严重危害。气候变化是影响生物和非生物方面的气候条件变化的一个连续的、多维的过程。这引起了气候环境的变化,包括极端高温、降雨量和温度,这导致了新型寄生虫、植被和疾病的增加,而蔬菜作物必须采用特定的方法,如生理、生化和分子过程,以确保在压力情况下的最佳发育。激素(植物激素)和渗透物的合成就是这些过程之一。植物激素是影响植物生长发育和细胞分化等生理过程的天然物质。在正常和逆境情况下,植物激素调节着必要的生理活动。植物激素有多种形式,在植物中发挥着重要的作用,无论是单独的还是相互配合的。这些物质在植物体内的种类和浓度对植物的活动和功能有重大影响。目前的研究描述了植物激素在压力条件下促进植物生长和发育的可能参与。
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引用次数: 1
Determination and comparisons of heavy metals (Cobalt and Iron) accumulation in muscle, liver, and gill tissues of Golden Mullet (Chelon aurata) in coastal areas of the Caspian Sea (Mazandaran and Golestan provinces of Iran) 里海沿海地区(伊朗Mazandaran和Golestan省)金鲻鱼(Chelon aurata)肌肉、肝脏和鳃组织中重金属(钴和铁)积累的测定和比较
Pub Date : 1900-01-01 DOI: 10.52547/injoere.3.1.1
B. Foroutan, H.R. Bashi Amlashi, A. Partani, P. Ríos, H. Nasrollahzadeh Saravi
The present research has aimed to figure out the amount of two heavy metals (Cobalt and Iron) accumulation, in muscle, liver, and gill tissues of Chelon aurata (golden mullet) in coastal districts of the Caspian Sea in two provinces of Iran and compare these data with their standard levels that advertised by some health organizations. It is because this species currently has the second-highest percentage of catch in the Iranian coastal of the Caspian Sea. Sampling mature Chelon aurata ( C. aurata ) was done in 6 stations of beach areas and 30 mature Chelon aurata were chosen to run the study. The biometric results illustrated that the highest recorded data of average body weight (BW), body length (BL), and fork length (FL) were related to gulden mullet which were caught at the coastal district of Behshahr station, however, the golden mullet fish caught at Gomishan beach had the lowest recorded figures of body weight ( p <0.05). The most amount of accumulation of Cobalt and Iron was recorded in Behshahr which showed a statistically significant difference with the other stands ( p <0.05). The liver, gill, and muscle samples had the most Cobalt and Iron accumulation, respectively (Liver>Gill>Muscle). According to the recorded data at present research, the accumulation balances of two heavy metals, Cobalt and Iron, were much lower than their announced standard ranges of World Health Organization (WHO), Food and Agricultural Organization (FAO), and United States Department of Agriculture (USDA). It is therefore recommended that utilization of this kind of fish which has grown under that environment is useless for human consumption.
本研究旨在测定伊朗里海两省沿海地区金鲻鱼肌肉、肝脏和鳃组织中两种重金属(钴和铁)的积累量,并将这些数据与一些卫生组织公布的标准水平进行比较。这是因为该物种目前在伊朗里海沿岸的捕捞率排名第二。在滩涂地区的6个站点采集了成熟的aurata (C. aurata),选取了30只成熟的Chelon aurata进行研究。生物特征分析结果表明,平均体重(BW)、体长(BL)和叉长(FL)记录值最高的是贝沙哈尔站沿海捕捞的金鲻鱼,而体重记录值最低的是戈米山海滩捕捞的金鲻鱼(p鳃>肌肉)。根据目前研究的记录数据,钴和铁这两种重金属的积累平衡远远低于世界卫生组织(世卫组织)、粮食及农业组织(粮农组织)和美国农业部(USDA)宣布的标准范围。因此,建议利用这种在这种环境下生长的鱼对人类消费是无用的。
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引用次数: 0
Comparative and regression analysis of Nile tilapia, Oreochromis niloticus (Linnaeus, 1758) (Family: Chchlidae) from Bugungu, Eastern region, Republic of Uganda, East Africa (0°23'31.92"N 33°13'51.95"E) 东非乌干达共和国东部布贡古地区(0°23′31.92”N 33°13′51.95”E)尼罗罗非鱼(Oreochromis niloticus, Linnaeus, 1758)(科:尼罗罗非鱼科)比较与回归分析
Pub Date : 1900-01-01 DOI: 10.52547/injoere.3.1.73
V. Thiruvengadam, Akinsorotan A.M.
Comparative and regression analysis of length-weight relationship of Oreochromis niloticus (Linneaus, 1785) for a period of six months from October 2020 to March 2021, Nile River, Bubungu, Eastern region, Uganda, East Africa, (0°23'31.92"N 33°13'51.95"E) (Fig 1). The slope value (b) estimated for O. niloticus of both sexes was found to be 3.4171. The regression equations derived for both sexes was estimated Log W=3.4171+0.1772 Log L. In spite of the fact that O. niloticus predetermined well in the wild collection of fish, there was a good statistical difference in their health and general wellbeing. The correlation coefficient was found to be ( p<0.01 ). The significant difference between sexes of the species " F " value was at 1% level. The b value differed from the ideal cube law of "3" as is with the case of LWR studied in this region. The slope value was compared to Indo-Pacific regions.
2020年10月至2021年3月,东非乌干达东部地区布邦古尼罗河(0°23′31.92”N 33°13′51.95”E) 6个月间nilochromis (Linneaus, 1785)的长度-重量关系比较与回归分析(图1)。nilochromis (O. niloticus)雌雄的斜率值(b)为3.4171。对两性的回归方程估计为Log W=3.4171+0.1772 Log L.尽管在野生鱼类中niloticus具有良好的预定性,但它们的健康状况和总体健康状况存在良好的统计差异。相关系数为(p<0.01)。不同性别间物种“F”值的显著差异在1%水平。b值与理想立方律“3”不同,本地区研究的LWR情况也是如此。坡度值与印度-太平洋地区进行了比较。
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引用次数: 0
Use of algal indices for determining of water quality in the Sirvan River tributaries (Kurdistan-Iran) 用藻类指数测定希尔万河支流水质(库尔德斯坦-伊朗)
Pub Date : 1900-01-01 DOI: 10.52547/injoere.3.1.79
A. Makhlough, H. Nasrollahzadeh Saravi, M. Naderi, F. Eslami, A. Ahmadnezhad
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引用次数: 0
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International Journal of Environmental Research and Education
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