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2009 International Symposium on Micro-NanoMechatronics and Human Science最新文献

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Pico-liter injection control to individual nano-liter solution coated by lipid layer 微升注射控制到单个纳升溶液涂有脂质层
Pub Date : 2009-12-11 DOI: 10.1109/MHS.2009.5351948
Y. Matsuno, M. Nakajima, M. Kojima, Y. Tanaka-Takiguchi, K. Takiguchi, K. Nogawa, M. Homma, T. Fukuda
This paper presents the evaluation of ultra-minimal spout amount from micro-nano pipettes into phospholipid-coated micro-droplets. The pipettes can be used to control the local environment around/inside single cells. Conventionally, the microinjection with pipette has been conducted by air pressures. In this method, the spout of high viscosity solutions is difficult because of the frictional forces between the surface of a pipette and a solution. It is also needed to evaluate the spout amount quantitatively. On the other hand, the research about artificial cell model has been actively conducted, injecting various biological samples into liposomes which are vesicle of lipid bilayer membrane. If additional injection of various proteins into liposome is realized with micro/nano pipette, the observation of dynamic reaction of multiple biological samples, which is required for formulation of artificial cell, will become possible. In our research, the spouting method using electro-osmosis is used. The local spouts of the proteins such as GFP and F-actin have been presented experimentally before. In this paper, the amount of spout solution in this method is quantitatively evaluated by injection of fluorescent solution into a phospholipid-coated micro-droplet.
本文介绍了从微纳移液器到磷脂包被微液滴的超最小喷出量的评价。移液器可用于控制单个细胞周围/内部的局部环境。传统上,用移液管进行显微注射是在气压下进行的。在这种方法中,由于移液管表面和溶液之间的摩擦力,高粘度溶液的喷射是困难的。还需要定量地评价喷淋量。另一方面,积极开展人工细胞模型的研究,将各种生物样品注射到脂质体中,脂质体是脂质双层膜的囊泡。如果用微纳移液器实现脂质体中各种蛋白的附加注射,就可以观察多种生物样品的动态反应,这是制备人工细胞所需要的。在我们的研究中,采用电渗透的喷射方法。GFP和f -肌动蛋白等蛋白的局部喷口已经在实验中被发现。本文通过将荧光溶液注射到磷脂包被的微滴中,定量地评价了该方法的喷液量。
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引用次数: 3
Development of a pH indicator immobilized-gel-sheet for microenviroment analysis 微环境分析用pH指示剂固定化凝胶片的研制
Pub Date : 2009-12-11 DOI: 10.1109/MHS.2009.5351885
T. Masuda, H. Maruyama, F. Arai, T. Anada, T. Fukuda, O. Suzuki
Detection of change in microenvironmental pH may be essential for analyzing biological processes, if transitory biodegradable scaffold materials are used for tissue engineering in bone regeneration. In this study, we created a pH indicator immobilized-gel-sheet for pH measurement. By using gel-sheet, we can investigate the local microenvironment of biodegradable materials, such as octacalcium phosphate (OCP), under the various protein adsorptions. The gel-sheet was made of a photocrosslinkable prepolymer solution (TUPR-5), which was easily immobilized by the method that the pH indicator is entrapped in a porous polymeric substrate. Bromothymol Blue (BTB) immobilized-gel-sheet was mounted in a biochip made of polydimethylsiloxane (PDMS) with a flow channel. The pH value was calculated by detecting the color changes, and presented as the pH distribution via a custom-made application. The pH sensing was carried out based on the surrounding color information of an OCP granule if incubated in culture medium. The gel-sheet detected the pH values of the condition around an OCP granule, showing the decrease from pH 7.8 to 8.8. As a whole, the proposed pH indicator is capable of providing pH level by high-spatial resolution.
如果暂时的可生物降解支架材料用于骨再生的组织工程,检测微环境pH值的变化可能是分析生物过程所必需的。在这项研究中,我们创建了一个pH指示剂固定化凝胶片用于pH测量。利用凝胶片,我们可以研究生物降解材料,如OCP,在不同蛋白质吸附下的局部微环境。凝胶片由可光交联的预聚物溶液(TUPR-5)制成,通过将pH指示剂包埋在多孔聚合物衬底上的方法易于固定化。将溴百里酚蓝(BTB)固定化凝胶片安装在具有流道的聚二甲基硅氧烷(PDMS)生物芯片上。通过检测颜色变化计算pH值,并通过定制应用程序表示为pH分布。根据OCP颗粒在培养基中孵育的周围颜色信息进行pH感应。凝胶片检测了OCP颗粒周围环境的pH值,pH值从7.8下降到8.8。总体而言,所提出的pH指示剂能够提供高空间分辨率的pH水平。
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引用次数: 4
MEMS with five senses for IRT 具有五感IRT的MEMS
Pub Date : 2009-12-11 DOI: 10.1109/MHS.2009.5352101
I. Shimoyama
IRT is a new technological domain created by the integration of Information Technology (IT), which encompasses high-speed computing and network and software technologies, and Robot Technology (RT), whose forms and movements function in the physical world. IT, formed from technologies of the cyber-world, has the power to process information in the real world through communication and computer technologies. It promises to have even more advanced capabilities in the future. RT, formed from the technologies of the physical world, has advanced greatly in reliability and real-world functionality. By integration IT and RT, IRT innovations promise to give birth to industries that support life and society, and to bring about social transformation. One of the killer applications of IRT is a kitchen robot. The robot we have developed has tactile sensors to deal with plates and glasses on a tray and put them into a dishwasher. The tactile sensors are molded by elastic materials with tiny cantilevers in it. Not only tactile sensors but also vision/acoustic sensors are necessary for motion, safety and reliability of household robots. MEMS (MicroElectroMechanical Systems) give tiny/thin sensor arrays which cover a robot as smart skin.
IRT是包含高速计算、网络和软件技术的信息技术(IT)与形式和运动在物理世界中起作用的机器人技术(RT)相结合而形成的新技术领域。信息技术由网络世界的技术形成,具有通过通信和计算机技术处理现实世界信息的能力。它承诺在未来拥有更先进的功能。RT是由物理世界的技术形成的,在可靠性和现实世界的功能方面有了很大的进步。通过IT和RT的融合,IRT创新有望催生支持生活和社会的产业,并带来社会变革。IRT的杀手级应用之一是厨房机器人。我们开发的机器人有触觉传感器,可以处理托盘上的盘子和玻璃杯,并将它们放入洗碗机。触觉传感器是由带有微小悬臂的弹性材料制成的。为了保证家用机器人的运动、安全、可靠,不仅需要触觉传感器,还需要视觉/声学传感器。MEMS(微机电系统)提供微小/薄的传感器阵列,覆盖机器人作为智能皮肤。
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引用次数: 0
Adsorbed surfactant thickness on: A Si wafer dominating etching properties of TMAH solution 硅片表面活性剂的厚度决定了TMAH溶液的蚀刻性能
Pub Date : 2009-12-11 DOI: 10.1109/MHS.2009.5352098
B. Tang, M. Gosálvez, P. Pal, S. Itoh, H. Hida, M. Shikida, Kazuo Sato
The goal of this article is to study the etching properties as a function of various adsorbed surfactant thickness in wet anisotropic etching process of TMAH solution. The thickness of preferentially adsorbed surfactant molecules on Si{110} and Si{100} has been evaluated by using spectroscopic ellipsometry (SE). The dependence of the etch rate in TMAH and the surface roughness on the layer thickness demonstrates that the surfactant is adsorbed at the interface during etching in TMAH+Triton. A thin pre-adsorbed layer is sufficient to dramatically improve the etching characteristics of silicon.
本文的目的是研究TMAH溶液湿法各向异性刻蚀过程中不同表面活性剂厚度对刻蚀性能的影响。利用椭圆偏振光谱(SE)研究了表面活性剂分子在Si{110}和Si{100}表面的优先吸附厚度。TMAH中蚀刻速率和表面粗糙度随层厚的变化关系表明,表面活性剂在TMAH+Triton中蚀刻时被吸附在界面上。薄的预吸附层足以显著改善硅的蚀刻特性。
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引用次数: 5
Development of the maskless photolithography device with an LCD-projector for fabrication of micropatterned surfaces 带lcd投影仪的无掩模光刻装置的研制
Pub Date : 2009-12-11 DOI: 10.1109/MHS.2009.5351890
K. Itoga, J. Kobayashi, M. Yamato, T. Okano
We developed the maskless photolithography device by modifying the optical system of an LCD projector and applied to cell micropatterning and fabrication of microchannels. Furthermore, we also developed the second-generation device allows for the fabrication of micropatterns over a larger area (over 50 × 50 mm). The maskless system has the big merit that doesn't need a photomask. However, there are a few problems in the maskless system. One of them is that patterns of segmentalized boundaries with an XY stage get out of shape. It is caused by optical distortion, precision of XY stage and so on. To overcome the defect, we have developed a third-generation device equipped with a more precision XY-stage and invented a method to improve patterns of divided edge by multiphase exposure. We were able to improve patterns of divided edge by the method.
我们通过改进LCD投影机的光学系统,开发了无掩模光刻装置,并将其应用于细胞微图像化和微通道的制造。此外,我们还开发了第二代设备,允许在更大的区域(超过50 × 50毫米)上制造微图案。无掩模系统的一大优点是不需要掩模。然而,无掩模系统也存在一些问题。其中之一是带有XY阶段的分割边界模式会变形。它是由光学畸变、XY台精度等因素引起的。为了克服这一缺陷,我们开发了配备更精确的x -stage的第三代器件,并发明了一种通过多相曝光改善分边图案的方法。用该方法改进了分边图案。
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引用次数: 0
On-chip particle-laden droplet dispensing by disposable inkjet system 一次性喷墨系统的片上微粒负载液滴点胶
Pub Date : 2009-12-11 DOI: 10.1109/MHS.2009.5351878
T. Mizunuma, Y. Yamanishi, S. Sakuma, H. Maruyama, F. Arai
We succeeded in dispensing micro-droplet by a disposable on-chip inkjet mechanism. Novelty of this paper is summarized as follows. (1) We employed a glass plate bonded Poly-dimethylsiloxane (PDMS) membrane to obtain leaf spring structure whose spring coefficient is 14 times of the conventional PDMS membrane. As a result, we succeeded in dispensing droplets continuously (up to 10 Hz) by vibrating the membrane using the multilayers piezoelectric actuator attached to the PDMS disposable chip. (2) The nozzle for droplet dispensing was fabricated by thick photoresist (SU-8) to obtain the hydrophobic surface which prevents any undesired satellite droplets produced. Consequently the accuracy of position of the droplet dispensing was achieved ± 5 μm. The size of the droplet produced from the disposable nozzle (diameter = 100 μm) was in the range of 95–105 μm at the applied voltage of 105 V.
我们成功地利用一次性片上喷墨机构进行微滴点胶。本文的新颖性总结如下。(1)采用玻璃板粘结聚二甲基硅氧烷(PDMS)膜,得到弹簧系数为常规PDMS膜14倍的钢板弹簧结构。结果,我们成功地通过使用附着在PDMS一次性芯片上的多层压电致动器振动膜来连续点胶液滴(高达10 Hz)。(2)采用厚光刻胶(SU-8)制作液滴点胶喷嘴,获得疏水表面,防止产生不需要的卫星液滴。结果表明,液滴点胶的位置精度为±5 μm。在105 V电压下,一次性喷嘴(直径为100 μm)产生的液滴尺寸在95 ~ 105 μm之间。
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引用次数: 2
Fabrication of biodegradable scaffold by powder sintering process 粉末烧结法制备可生物降解支架
Pub Date : 2009-12-11 DOI: 10.1109/MHS.2009.5351879
T. Itoyama, T. Nakano, S. Ikeda, T. Fukuda, T. Matsuda, M. Negoro, F. Arai
To reproduce blood vessel, we proposed new process for fabricating biodegradable scaffold by powder sintering process. In this process, model for molding scaffold was materialized by rapid prototyping. Biodegradable polymer powder and porogen were dusted to model and heated. So, arbitrary shape scaffold would be fabricated. Also, porosity that influences compliance of blood vessel scaffold would be adjusted by changing ratio of the polymer powder and porogen. We studied fabrication condition of blood vessel scaffold by measuring porosity and Young's modulus when the ratio of the polymer powder and porogen was adjusted. Also, HUVECs were cultured on the scaffold, and the scaffold's biocompatibility was confirmed.
为了复制血管,我们提出了粉末烧结法制备生物可降解支架的新工艺。在此过程中,采用快速成型技术实现了支架成型模型的物化。将可生物降解的聚合物粉末和多孔材料撒入模型并加热。因此,任意形状的支架将被制造出来。通过改变聚合物粉末与孔隙率的比例,调节孔隙率对血管支架顺应性的影响。通过测量聚合物粉末与孔隙率配比不同时的孔隙率和杨氏模量,研究了血管支架的制备条件。并在支架上培养huvec,证实支架的生物相容性。
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引用次数: 0
Skeletal muscle tissue engineering using functional magnetite nanoparticles 利用功能性磁铁矿纳米颗粒进行骨骼肌组织工程
Pub Date : 2009-12-11 DOI: 10.1109/MHS.2009.5351986
A. Ito, Hirokazu Akiyama, Yasunori Yamamoto, Y. Kawabe, M. Kamihira
Skeletal muscular tissues were constructed using magnetic force-based tissue engineering (Mag-TE) techniques. Mouse myoblast C2C12 cells labeled with magnetite cationic liposomes (MCLs) were seeded into a well of 24-well ultra-low cell attachment culture plates. When a magnet was positioned underneath the well, cells accumulated evenly onto the culture surface and formed a multilayered cell sheet. Furthermore, because an angiogenic potential of transplants is considered to be important for the long-term maintenance of cell survival and tissue functions, a vascular endothelial growth factor (VEGF) gene-modified C2C12 (C2C12/VEGF) cell sheets were also fabricated by the Mag-TE technique. The secretion level of C2C12/VEGF sheets was 3.0 ng/day, indicating that VEGF gene-expressing cell sheets were successfully fabricated. Since the shape of artificial tissue constructs can be controlled by magnetic force, a cellular string-like assembly was formed by placing a linear-shaped magnetic field concentrator with a magnet. These cellular sheets and strings shrank and did not maintain their shapes for an additional in vitro culture period during myogenic differentiation. On the other hand, when a silicone plug was positioned at the center of well during the fabrication of cell sheets, the cell sheets shrank and formed a ring-like assembly around the plug. After 6-d cultivation of cell rings in differentiation medium, the C2C12 cells differentiated to form multinucleated myotubes. Thus, these procedures can provide a novel strategy for skeletal muscular tissue engineering.
采用磁力组织工程技术构建骨骼肌组织。用磁性阳离子脂质体(mcl)标记的小鼠成肌细胞C2C12细胞接种于24孔超低细胞附着培养板中。当磁铁放置在孔下时,细胞均匀地聚集在培养表面并形成多层细胞片。此外,由于移植的血管生成潜力被认为对细胞存活和组织功能的长期维持很重要,因此我们还利用mage -te技术制备了血管内皮生长因子(VEGF)基因修饰的C2C12 (C2C12/VEGF)细胞片。C2C12/VEGF片的分泌量为3.0 ng/d,表明VEGF基因表达细胞片制备成功。由于人工组织结构的形状可以由磁力控制,因此通过将线形磁场集中器与磁铁放置在一起形成细胞弦状组装体。在肌源性分化过程中,这些细胞片和细胞串收缩,并不能在体外培养期间保持其形状。另一方面,在制造电池片的过程中,当硅塞被放置在井的中心位置时,电池片会收缩,并在塞周围形成环状组合。细胞环在分化培养基中培养6 d后,C2C12细胞分化形成多核肌管。因此,这些方法可以为骨骼肌组织工程提供一种新的策略。
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引用次数: 0
Microactuator using tensile thin film 微致动器采用拉伸薄膜
Pub Date : 2009-12-11 DOI: 10.1109/MHS.2009.5351764
M. Sasaki
The thin film is advantageous for preparing the compliant spring and structures with little mass. The surface micromachining can take these advantages. Since the film structures suffer from the bending generated by the stress imbalance inside, the bulk micromachining is now frequently used. When the tensile stress is introduced in the thin film combining with bulk micromachining, the structural robustness will be obtained. As for the torsion bar, the compliant spring performance can be obtained for the twisting motion keeping the rigidity against the other motions. The potentials are indicated with the micromirror. The performances of the low-voltage driving, temperature stability, possibility for increasing the resonant frequency by decreasing the mass of the moving element, and the wireless driving using the subtle energy generated by the electromagnetic induction are confirmed.
该薄膜有利于制备柔性弹簧和小质量结构。表面微加工可以利用这些优点。由于薄膜结构受到内部应力不平衡产生的弯曲的影响,体微加工现在被广泛使用。在薄膜中引入拉应力并结合体微加工,可获得结构的鲁棒性。对于扭杆,在扭转运动中可以获得柔顺弹簧性能,对其他运动保持刚度。电势用微镜显示。验证了其低压驱动性能、温度稳定性、通过减小运动元件质量提高谐振频率的可能性以及利用电磁感应产生的微小能量进行无线驱动的可行性。
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引用次数: 0
A new decision making criteria of ROI evaluation in video sequences and comparison with human evaluation psychology 一种新的视频序列ROI评价决策准则及其与人类评价心理的比较
Pub Date : 2009-12-11 DOI: 10.1109/MHS.2009.5351755
M. Rokunuzzaman, K. Sekiyama, T. Fukuda
This paper introduces a new decision making criteria by which the Region of Interest (ROI) is selected and evaluated. This criterion is based on Human psychology of relevance in visual perception. The experimental results are validated by comparing with Eye tracker system and Human evaluation measured by Subjective Correlation value for each ROI.
本文提出了一种新的决策准则,用以选择和评价感兴趣区域(ROI)。这一标准是基于人类视觉感知中的关联心理。通过对比眼动仪系统和人类对每个ROI主观相关值的评价,验证了实验结果。
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引用次数: 0
期刊
2009 International Symposium on Micro-NanoMechatronics and Human Science
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