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2009 International Symposium on Micro-NanoMechatronics and Human Science最新文献

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Wheelchair driving control with sway suppression of passenger's posture and evaluation of comfortable ride by emotional sweating 轮椅驾驶控制与乘客姿态的摇摆抑制及情绪出汗对乘坐舒适性的评价
Pub Date : 2009-12-11 DOI: 10.1109/MHS.2009.5351845
Y. Noda, Daisuke Yamagami, K. Terashima
This paper presents an advanced control system for a driving control system of an electrical wheelchair. The aim of the control system is to make possible a comfortable ride in the wheelchair. For this, a passenger model that uses a backrest in the wheelchair has been constructed and a driving pattern that suppresses a passenger's posture behavior has been developed using optimization methods. Then, the quality of the ride whiles a passenger drives the wheelchair has been evaluated by a questionnaire and by measurements of emotional sweating. In comparison with the conventional driving pattern, the proposed driving pattern suppresses about half of the passenger's previous posture behavior and improves the comfort of wheelchair driving.
介绍了一种电动轮椅驱动控制系统的先进控制系统。控制系统的目的是使轮椅的乘坐舒适成为可能。为此,构建了使用轮椅靠背的乘客模型,并利用优化方法开发了抑制乘客姿势行为的驾驶模式。然后,通过问卷调查和情绪出汗测量来评估乘客驾驶轮椅时的乘坐质量。与传统的驾驶模式相比,所提出的驾驶模式抑制了大约一半的乘客先前的姿势行为,提高了轮椅驾驶的舒适性。
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引用次数: 0
Three dimensional bipedal walking locomotion using dynamic passivization of joint control 基于动态钝化关节控制的三维双足行走运动
Pub Date : 2009-12-11 DOI: 10.1109/MHS.2009.5351747
Minoru Ishida, Shohei Kato, M. Kanoh, H. Itoh
A central pattern generator (CPG) and passive dynamic walking (PDW) have attracted much attention in the research field of bipedal locomotion. We describe a motion control method based on dynamic joint passivization for biped robot locomotion. CPG-based motion control is effective for walking on uneven terrain. However, it has serious problems with energy loss. In contrast, PDW saves energy because a robot can walk without any active control or energy input on a downhill slope. However, PDW robot can not walk on uneven terrain, but only on a downhill slope. We think that active walking needs to be mixed with PDW for robot walking. Our motion control method is based on a mixture of the CPG and PDW, that is, the dynamic passivization of joint control. Experiments using the motion control method based on dynamic passivization of joint control successfully generated energy efficient walking and enabled superior gaits.
中心模式发生器(CPG)和被动动态行走(PDW)是两足运动研究领域的热点。提出了一种基于动态关节钝化的双足机器人运动控制方法。基于cpg的运动控制对于在不平坦地形上行走是有效的。然而,它有严重的能量损失问题。相比之下,PDW节省了能量,因为机器人可以在没有任何主动控制或能量输入的情况下在下坡上行走。然而,PDW机器人不能在不平坦的地形上行走,只能在下坡上行走。我们认为主动步行需要与PDW相结合。我们的运动控制方法是基于CPG和PDW的混合,即关节控制的动态钝化。采用基于关节控制动态钝化的运动控制方法进行实验,成功地产生了节能步行,实现了优越的步态。
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引用次数: 1
Study on an efficient fabrication process for PMMA movable microstructures based on hot embossing and polishing processes 基于热压印和抛光工艺的PMMA可动微结构高效制造工艺研究
Pub Date : 2009-12-11 DOI: 10.1109/MHS.2009.5351742
Satoshi Amava, D. Dao, S. Sugiyama
This paper reports our study on an efficient fabrication process for polymer microstructures utilizing hot embossing and polishing. In this paper, PMMA micro movable structures are set as the target. First, a silicon mold is fabricated by bulk micromachining technology. Next, PMMA microstructures are formed on PMMA plate by hot embossing process. Then, the hot-embossed structures are bonded to a PMMA substrate, and the backside layer of the hot-embossed PMMA structure that remained after hot embossing is removed by polishing to release the movable structures. PMMA movable microstructures with line and space of 10 µm and 5 µm, respectively, and the thickness of about 60 µm have been fabricated successfully. This is a low-cost and highly efficient method to fabricate polymer MEMS devices.
本文报道了一种利用热压印和抛光技术制备聚合物微结构的高效工艺。本文以PMMA微活动结构为研究对象。首先,采用本体微加工技术制备硅模具。其次,通过热压成型工艺在PMMA板上形成PMMA微结构。然后,将热压结构粘合到PMMA基板上,并通过抛光去除热压后保留的热压PMMA结构的背面层,以释放可移动结构。成功制备出线距为10µm、间距为5µm、厚度约为60µm的PMMA可动微结构。这是一种低成本、高效率的制造聚合物MEMS器件的方法。
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引用次数: 2
Evolutionary artificial potential field method based manipulator path planning for safe robotic assembly 基于进化人工势场法的机器人安全装配机械手路径规划
Pub Date : 2009-12-11 DOI: 10.1109/MHS.2009.5352075
Fei Chen, P. Di, Jian Huang, H. Sasaki, T. Fukuda
To design a safe path planning for the manipulator is a key issue during the human worker and manipulator cooperation cell assembly. In this study, a cell assembly system involving human worker and manipulator is designed. According to the camera vision information, the locations of the human worker and the manipulator are calculated, and then the potential field is calculated based on the Artificial Potential Field (APF) method. In order to generate a semi-optimal safe path planning for the manipulator, a criterion is imported to evaluate the path and an Evolutionary Algorithm (EA) is adopted to adjust the parameters to meet this criterion. The effectiveness of these methods is finally confirmed through simulation experiments.
机械手的安全路径规划设计是人-机械手协同单元装配过程中的一个关键问题。在本研究中,设计了一个包含工人和机械手的细胞装配系统。根据摄像机视觉信息,计算工作人员和机械手的位置,然后基于人工势场(APF)法计算势场。为了生成机械臂的半最优安全路径规划,引入了评估路径的准则,并采用进化算法调整参数以满足该准则。最后通过仿真实验验证了这些方法的有效性。
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引用次数: 21
Thermal gel actuated device for spout/suction inside semi-closed microchip 半闭式微芯片内的热凝胶驱动喷口/吸口装置
Pub Date : 2009-12-11 DOI: 10.1109/MHS.2009.5351943
Masaru Takeuchi, M. Nakajima, T. Fukuda
In this paper, we propose a thermal gel actuated probe which uses the volume change of the thermal gel as an actuator. The thermal gel actuated probe can be used for single cell analysis, especially single cell manipulation. The phase change from sol to gel causes an increase in volume, while the change from gel to sol causes a decrease in volume. The thermal gel actuated probe can spout and suck solution by these volume changes. This thermal gel actuated probe can be used as a disposable device. The thermal gel actuated probe also realize easy to use because it is needed only to switch the heater turning on and off for spout and suction of solution. The volume change of the thermal gel was evaluated. It is also evaluated that the volume of spouted solution by the thermal gel actuated probe. Finally, the target single microbead suction and spout were demonstrated by the thermal gel actuated probe. It is considered that the thermal gel actuated probe can realize the target cell manipulation, and can contribute to the single cell analysis.
本文提出了一种利用热凝胶的体积变化作为致动器的热凝胶致动探针。热凝胶驱动探针可用于单细胞分析,特别是单细胞操作。从溶胶到凝胶的相变使体积增大,而从凝胶到溶胶的相变使体积减小。热凝胶驱动探针可以通过这些体积变化喷出和吸入溶液。这种热凝胶驱动探针可以作为一次性设备使用。热凝胶驱动探针还实现了易于使用,因为它只需要打开和关闭加热器的喷嘴和吸入溶液。对热凝胶的体积变化进行了评价。并对热凝胶驱动探针喷出溶液的体积进行了评价。最后,利用热凝胶驱动探针对目标单微珠的吸力和喷出进行了验证。认为热凝胶驱动探针可以实现靶细胞的操作,有助于单细胞分析。
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引用次数: 1
Shear stress induces arterial differentiation of bone marrow-derived endothelial progenitor cells 剪切应力诱导骨髓源性内皮祖细胞动脉分化
Pub Date : 2009-12-11 DOI: 10.1109/MHS.2009.5351856
S. Obi, Kimiko Yamamoto, Tomomi Masumura, T. Asahara, J. Ando
Endothelial progenitor cells (EPCs) are mobilized from bone marrow to peripheral blood, and contribute to angiogenesis in tissues. In the process EPCs are exposed to the shear stress generated by blood flow and tissue fluid flow. Our previous study showed that shear stress promotes differentiation of EPCs into mature endothelial cells. In this study we investigated whether EPCs differentiate into arterial or venous endothelial cells in response to shear stress. When cultured EPCs derived from human peripheral blood were exposed to controlled levels of shear stress in a flow-loading device, the mRNA levels of the arterial endothelial cell markers ephrinB2, Notch1/3, Hey1/2, and ALK1 increased, but the mRNA levels of the venous endothelial cell markers EphB4 and NRP2 decreased. Both the ephrinB2 increase and the EphB4 decrease were shear-stress- rather than shear-rate-dependent. EphrinB2 protein was increased in shear-stressed EPCs, and the increase in ephrinB2 expression was due to activated transcription and not mRNA stabilization. Deletion analysis of the ephrinB2 promoter indicated that the cis-element (shear stress response element) is present within 106 bp 5' upstream from the transcription initiation site. This region contains the Sp1 consensus sequence, and a mutation in its sequence decreased the basal level of transcription and abolished shear stress-induced ephrinB2 transcription. Electrophoretic mobility shift assays and chromatin immunoprecipitation assays showed that shear stress markedly increased binding of Sp1 to its consensus sequence. These results indicate that shear stress induces differentiation of EPCs into arterial endothelial cells by increasing ephrinB2 expression in EPCs through Sp1 activation.
内皮祖细胞(Endothelial progenitor cells, EPCs)从骨髓转移到外周血,参与组织血管生成。在此过程中,内皮祖细胞暴露于血流和组织液流动产生的剪切应力中。我们之前的研究表明,剪切应力促进EPCs向成熟内皮细胞的分化。在这项研究中,我们研究了EPCs是否在剪切应力的作用下分化为动脉或静脉内皮细胞。当体外培养的人外周血内皮细胞在流量负荷装置中受到控制水平的剪切应力时,动脉内皮细胞标记物ephrinB2、Notch1/3、Hey1/2和ALK1的mRNA水平升高,而静脉内皮细胞标记物EphB4和NRP2的mRNA水平降低。ephrinB2的升高和EphB4的降低均与剪切应力有关,而与剪切速率无关。剪切胁迫下EPCs中EphrinB2蛋白表达增加,其表达增加是由于转录激活而非mRNA稳定。对ephrinB2启动子的缺失分析表明,顺式元件(剪切应力响应元件)存在于转录起始位点上游106 bp 5'处。该区域包含Sp1一致序列,该序列的突变降低了转录的基础水平,并消除了剪切应力诱导的ephrinB2转录。电泳迁移率转移试验和染色质免疫沉淀试验表明,剪切应力显著增加Sp1与其一致序列的结合。这些结果表明,剪切应力通过Sp1激活增加EPCs中ephrinB2的表达,诱导EPCs向动脉内皮细胞分化。
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引用次数: 1
Use of cytometry technology for the study of stem cell biology 利用细胞术技术研究干细胞生物学
Pub Date : 2009-12-11 DOI: 10.1109/MHS.2009.5352042
H. Nakauchi
Hematopoietic stem cells (HSCs) are best-studied stem cells and they have contributed a great deal for the development of stem cell biology. HSCs reside in a bone marrow niche in a non-dividing state from which they occasionally are aroused to undergo cell division. Using highly purified HSCs by flow cytometry, we have recently shown that cytokine treatment of HSCs led to polarization of the lipid raft marker GM1 ganglioside as well as to phosphorylation of Akt and relocation of FOXO3a to the cytoplasm. Lipid raft clustering induced by cytokines is essential for HSC re-entry into the cell cycle. Furthermore, based on a hypothesis that signals from the niche inhibit LRC and induce hibernation in HSCs, we screened candidate niche signals for inhibition of LRC. Among niche signals examined, transforming growth factor-β (TGF-β) efficiently inhibits LRC and induces p57Kip2 expression, leading to HSC hibernation ex vivo. These data establish the role of TGF-β as a niche signal in control of HSC hibernation and provide important clues to identify stem cell niche in bone marrow.
造血干细胞(Hematopoietic stem cells, hsc)是目前研究最多的干细胞,为干细胞生物学的发展做出了巨大贡献。造血干细胞以非分裂状态存在于骨髓壁龛中,偶尔会被唤醒进行细胞分裂。通过流式细胞术使用高度纯化的造血干细胞,我们最近发现细胞因子处理造血干细胞导致脂筏标记物GM1神经节苷脂的极化以及Akt的磷酸化和FOXO3a向细胞质的转移。细胞因子诱导的脂筏聚集是HSC重新进入细胞周期的必要条件。此外,基于来自生态位信号抑制LRC并诱导hsc冬眠的假设,我们筛选了抑制LRC的候选生态位信号。在检测的生态位信号中,转化生长因子-β (TGF-β)有效抑制LRC并诱导p57Kip2表达,导致HSC在体外冬眠。这些数据确立了TGF-β作为一个生态位信号在控制HSC冬眠中的作用,为鉴定骨髓干细胞生态位提供了重要线索。
{"title":"Use of cytometry technology for the study of stem cell biology","authors":"H. Nakauchi","doi":"10.1109/MHS.2009.5352042","DOIUrl":"https://doi.org/10.1109/MHS.2009.5352042","url":null,"abstract":"Hematopoietic stem cells (HSCs) are best-studied stem cells and they have contributed a great deal for the development of stem cell biology. HSCs reside in a bone marrow niche in a non-dividing state from which they occasionally are aroused to undergo cell division. Using highly purified HSCs by flow cytometry, we have recently shown that cytokine treatment of HSCs led to polarization of the lipid raft marker GM1 ganglioside as well as to phosphorylation of Akt and relocation of FOXO3a to the cytoplasm. Lipid raft clustering induced by cytokines is essential for HSC re-entry into the cell cycle. Furthermore, based on a hypothesis that signals from the niche inhibit LRC and induce hibernation in HSCs, we screened candidate niche signals for inhibition of LRC. Among niche signals examined, transforming growth factor-β (TGF-β) efficiently inhibits LRC and induces p57Kip2 expression, leading to HSC hibernation ex vivo. These data establish the role of TGF-β as a niche signal in control of HSC hibernation and provide important clues to identify stem cell niche in bone marrow.","PeriodicalId":344667,"journal":{"name":"2009 International Symposium on Micro-NanoMechatronics and Human Science","volume":"35 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2009-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127539365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genomic SELEX for the genome-wide search of regulation targets by transcription factors: SELEX-clos and SELEX-chip procedures 基因组SELEX用于转录因子调控靶标的全基因组搜索:SELEX闭合和SELEX芯片程序
Pub Date : 2009-12-11 DOI: 10.1109/MHS.2009.5351954
T. Shimada, N. Fujita, Kaneyoshi Yamamoto, A. Ishihama
The pattern of genome transcription in prokaryotes is determined by selective distribution of RNA polymerase within the genome. The gene selectivity of RNA polymerase is modulated after interaction with DNA-binding transcription factors (TFs). Escherichia coli contains a total of about 300 TFs, but the regulatory function has not yet been identified for about one third. For quick and systematic search of the regulation target genes by TFs, we have developed a novel screening technology “Genomic SELEX”, in which the recognition DNA sequences by the test TF are isolated from a mixture of genome DNA fragments. The sequences were determined by either cloning-sequencing of SELX fragments (SELEX-clos) or high-density microarray (tilling array) analysis (SELEX-chip). Here we describe the application of these novel technologies for search of the whole set of regulation target genes on the E. coli genome by CRP (cAMP-binding protein).
原核生物基因组转录的模式是由基因组内RNA聚合酶的选择性分布决定的。RNA聚合酶与dna结合转录因子(TFs)相互作用后,基因的选择性受到调控。大肠杆菌共含有约300个TFs,但其中约三分之一的调节功能尚未确定。为了快速系统地利用TF搜索调控靶基因,我们开发了一种新的筛选技术“Genomic SELEX”,该技术从混合基因组DNA片段中分离出被测试TF识别的DNA序列。通过SELX片段克隆测序(SELEX-clos)或高密度微阵列(tilling array)分析(SELEX-chip)确定序列。本文描述了这些新技术在利用CRP (camp结合蛋白)搜索大肠杆菌基因组上整套调控靶基因方面的应用。
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引用次数: 1
Associative motion generation for humanoid robots based on analogy with indication 基于指示类比的仿人机器人联想运动生成
Pub Date : 2009-12-11 DOI: 10.1109/MHS.2009.5352007
Satona Motomura, Shohei Kato, H. Itoh
We describe a method of generating new motions associatively from unfamiliar indications. The associative motion generation system is composed of two neural networks: nonlinear principal component analysis (NLPCA) and Jordan recurrent neural network (JRNN). First, the system learns the correspondence relationship between an indication and a motion using training data. Second, associative values are extracted for associating a new motion from an unfamiliar indication using NLPCA. Last, the robot generates a new motion through calculation by JRNN using the associative values. Experimental results demonstrated that our method enabled a humanoid robot, KHR-2HV, to associatively generate some kinds of motion depending on given unfamiliar indications.
我们描述了一种从不熟悉的指示联想产生新动作的方法。联想运动生成系统由非线性主成分分析(NLPCA)和Jordan递归神经网络(JRNN)两个神经网络组成。首先,系统使用训练数据学习指示和动作之间的对应关系。其次,使用NLPCA提取关联值,用于从不熟悉的指示中关联新运动。最后,通过JRNN算法计算机器人的关联值,生成新的运动。实验结果表明,我们的方法使类人机器人KHR-2HV能够根据给定的不熟悉的指示联想地产生某些类型的运动。
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引用次数: 0
Microfluidic chip with world-to-chip interface for temperature detection in micro-nanoscale 微流控芯片与世界芯片之间的接口,用于微纳米尺度的温度检测
Pub Date : 2009-12-11 DOI: 10.1109/MHS.2009.5351965
N. Inomata, H. Maruyama, Takahiro Kato, F. Arai
In this paper, we proposed a microfluidic chip having a world-to-chip interface for multi-scale environmental measurement on a chip. There are several techniques for environmental measurement of the microscale objects using cantilever, microsensor and fluorescence method etc. Each technique has useful advantages against other methods. To employ these methods on a microfluidic chip simultaneously, we aim to develop a world-to-chip interface to insert a cantilever into the microfluidic chip and position it for sensing in a chip. We fabricated the microfluidic chip having this interface to insert the cantilever. This chip was designed to be used for inverted microscope. The solution does not leak from the interface by employing simultaneous flow control at both inlet and drain port. By using this interface, we demonstrated insertion and positioning of the cantilever into the microfluidic chip, and confirmed two-layer laminar flow control and detection of the local temperature change in the microchannel.
本文提出了一种具有世界对芯片接口的微流控芯片,用于芯片上的多尺度环境测量。微尺度物体的环境测量技术有悬臂梁法、微传感器法和荧光法等。与其他方法相比,每种技术都具有有用的优势。为了在微流控芯片上同时应用这些方法,我们的目标是开发一个世界到芯片的接口,将悬臂插入微流控芯片并将其定位在芯片中进行传感。我们制作了具有该界面的微流控芯片来插入悬臂梁。该芯片是为倒置显微镜设计的。通过在进口和排放口同时进行流量控制,溶液不会从界面泄漏。通过该接口,我们演示了悬臂梁插入和定位到微流控芯片中,并验证了微通道中两层层流控制和局部温度变化的检测。
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引用次数: 1
期刊
2009 International Symposium on Micro-NanoMechatronics and Human Science
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