Bioprinting最新文献_第7页

Fabrication of 3D soft polymeric constructs at high structural integrity through bioprinting optimization of suspended hydrogels 通过生物打印优化悬浮水凝胶制备高结构完整性的三维软聚合物结构

Q1 Computer Science

Bioprinting

Pub Date : 2025-03-11 DOI: 10.1016/j.bprint.2025.e00403

Miriam Seiti , Elena Laura Mazzoldi , Stefano Pandini , Eleonora Ferraris , Paola Serena Ginestra

In vitro models of soft tissues, such as neural, vitreous, or hematopoietic human tissues, require three-dimensional (3D), soft, and functionalized constructs that mimic the complex extracellular microenvironment and support tissue growth and differentiation. While bioprinting has gained significant interest in bioengineering, there is limited research on process control for the biomanufacturing of soft tissues, which is still in its early stages. Material extrusion of suspended hydrogels has shown promise in processing low-viscosity inks, but challenges in developing bioinks that maintain good shape fidelity, repeatability, and long-term stability in culture media have slowly progressed. In this study, we optimize the bioprinting process for the suspended extrusion of low-viscosity autoclaved bioinks (η = 121 ± 4 mPa s from 50 to 100 s⁻¹) into 3D soft polymeric constructs. The stability of the process is evaluated using a full factorial design approach. Bone marrow-derived stromal cells are bioprinted at a low concentration (5 × 10⁵ cells/mL), showing excellent metabolic activity up to day 7 compared to 2D cell culture controls. The final soft constructs exhibit a compression Young's Modulus of 7.8 ± 0.9 kPa, a water uptake of 60 %, and minimal gel degradation over 21 days. This work offers new insights into optimizing this advanced bioprinting process towards the development and study of 3D in vitro soft tissue models.

软组织的体外模型，如神经、玻璃体或造血人体组织，需要三维（3D）、柔软和功能化的结构，以模拟复杂的细胞外微环境并支持组织生长和分化。虽然生物打印在生物工程领域引起了极大的兴趣，但对软组织生物制造过程控制的研究有限，仍处于早期阶段。悬浮水凝胶的材料挤压在处理低粘度油墨方面显示出前景，但在开发生物油墨方面的挑战，保持良好的形状保真度，可重复性和在培养基中的长期稳定性进展缓慢。在这项研究中，我们优化了生物打印工艺，将低粘度高压灭菌生物墨水（η = 121±4 mPa s，从50到100 s−1）悬浮挤出成三维软聚合物结构。采用全因子设计方法评价该工艺的稳定性。骨髓来源的基质细胞以低浓度（5 × 105个细胞/mL）进行生物打印，与2D细胞培养对照相比，在第7天显示出优异的代谢活性。最终的软结构的压缩杨氏模量为7.8±0.9 kPa，吸水率为60%，在21天内凝胶降解最小。这项工作为优化这种先进的生物打印工艺，开发和研究体外3D软组织模型提供了新的见解。

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引用次数: 0

Optimization of 3D printed Voronoi microarchitecture bone scaffold using Taguchi-grey relational analysis 应用田口灰关联分析优化3D打印Voronoi微结构骨支架

Q1 Computer Science

Bioprinting

Pub Date : 2025-03-08 DOI: 10.1016/j.bprint.2025.e00402

Rochmad Winarso , Sugeng Slamet , Rianto Wibowo , Sigit Arrohman , Akhmad Zidni Hudaya , Rifky Ismail , Jamari , Athanasius Priharyoto Bayuseno

3D printing bone scaffolds is a cutting-edge approach in bone tissue engineering, potentially resolving critical-sized bone defect challenges. While current research primarily focuses on tensile parameters in printing, compressive parameters are often overlooked despite their crucial role in scaffold performance. This study aimed to optimize the mechanical properties of bone scaffolds featuring Voronoi microarchitecture through tailored printing parameters. Utilizing the Taguchi method and Grey Relational Analysis (GRA), significant variations in mechanical parameters such as elastic modulus and compressive strength were identified among specimen groups. Key printing factors including layer height, line width, printing temperature, and printing speed proved pivotal in influencing the compressive strength and elastic modulus of polylactic acid (PLA) used in 3D printing. This research demonstrates the novelty of combining the Taguchi-GRA approach with the Voronoi microarchitecture to achieve superior mechanical properties. Specifically, optimal settings layer height of 0.0625 mm, line width of 0.25 mm, printing temperature of 215 °C, and printing speed of 55 mm/s, yielded scaffolds with enhanced compressive strength and elastic modulus, meeting biomechanical requirements for bone regeneration. Further investigation is warranted to establish comprehensive guidelines for achieving consistent mechanical excellence in 3D-printed PLA components, thereby advancing the efficacy and reliability of bone scaffold applications. The findings of this study provide a foundation for standardizing 3D printing protocols for bone scaffolds, bridging the gap between experimental designs and clinical applications. By addressing critical bottlenecks and introducing innovative solutions, this research contributes to advancing the field of bone tissue engineering and improving outcomes in regenerative medicine. patient outcomes in bone tissue engineering.

3D打印骨支架是骨组织工程的一种前沿方法，可能解决临界尺寸的骨缺陷挑战。虽然目前的研究主要集中在打印中的拉伸参数，但压缩参数往往被忽视，尽管它们在支架性能中起着至关重要的作用。本研究旨在通过定制打印参数，优化Voronoi微结构骨支架的力学性能。利用田口法和灰色关联分析（GRA），确定了试件组之间弹性模量和抗压强度等力学参数的显著变化。打印层高、线宽、打印温度和打印速度等关键打印因素对3D打印中使用的聚乳酸（PLA）的抗压强度和弹性模量具有关键影响。这项研究展示了将Taguchi-GRA方法与Voronoi微架构相结合以实现卓越机械性能的新颖性。其中，最佳设置层高0.0625 mm，线宽0.25 mm，打印温度215℃，打印速度55 mm/s，可获得抗压强度和弹性模量增强的支架，满足骨再生的生物力学要求。有必要进一步研究，以建立全面的指导方针，以实现3d打印PLA部件的一致机械性能，从而提高骨支架应用的有效性和可靠性。本研究结果为规范骨支架3D打印方案提供了基础，弥合了实验设计与临床应用之间的差距。通过解决关键瓶颈和引入创新的解决方案，本研究有助于推进骨组织工程领域和改善再生医学的成果。骨组织工程患者预后。

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引用次数: 0

Sterilizing bioinks: Understanding the impact of techniques on 3D bioprinting materials 灭菌生物墨水：了解技术对3D生物打印材料的影响

Q1 Computer Science

Bioprinting

Pub Date : 2025-02-21 DOI: 10.1016/j.bprint.2025.e00399

Lakshmi Menon , Dhruv Sanjanwala , Shivansh Sharma , Parul , Ratnesh Jain , Prajakta Dandekar

Natural polymers, such as alginate, chitosan, gelatin, and their derivatives, are widely used in formulating bioinks for 3D bioprinting of tissue engineering scaffolds. Due to their natural origin and biodegradable nature, these polymers are highly susceptible to microbial contamination, making effective sterilization crucial. This research paper provides a comprehensive analysis of the effects of various sterilization methods, namely, ultraviolet radiation, autoclaving, ethylene oxide treatment, membrane filtration, and lyophilization, on the physical and chemical properties, bioprinting performance, mechanical strength, and biocompatibility of these polymers. Additionally, experiments have been conducted to assess the impact of sterilization on commonly used viscosity enhancers, such as pectin, xanthan gum, and guar gum, bioactive nanofillers like montmorillonite and hydroxyapatite, and crosslinking agents like calcium chloride, citric acid, glutaraldehyde, and Irgacure 2959, which are other critical components in bioink formulations. The findings highlight that the choice of sterilization method should be tailored to the specific component, considering their physicochemical properties, applications, and practical convenience. This study involves a comprehensive examination of different sterilization techniques for several bioink components, highlighting the importance of selecting an appropriate method to ensure bioink stability. Unlike previous research, it offers a more extensive evaluation by covering a wide range of commonly used bioink constituents and examining the impact of diverse sterilization methods on their stability, thereby offering new insights into optimizing sterilization protocols for enhanced and reproducible bioprinting outcomes.

海藻酸盐、壳聚糖、明胶等天然聚合物及其衍生物被广泛应用于组织工程支架3D打印的生物墨水中。由于其天然来源和可生物降解的性质，这些聚合物极易受到微生物污染，因此有效的灭菌至关重要。本文全面分析了紫外线辐射、高压灭菌、环氧乙烷处理、膜过滤和冻干等多种灭菌方法对这些聚合物的理化性能、生物打印性能、机械强度和生物相容性的影响。此外，还进行了实验，以评估灭菌对常用的粘度增强剂（如果胶、黄原胶和瓜尔胶）、生物活性纳米填料（如蒙脱土和羟基磷灰石）和交联剂（如氯化钙、柠檬酸、戊二醛和Irgacure 2959）的影响。这些交联剂是生物墨水配方中的其他关键成分。研究结果强调，灭菌方法的选择应根据具体成分，考虑其物理化学性质，应用和实际便利性。本研究涉及对几种生物链成分的不同灭菌技术的全面检查，强调选择适当方法以确保生物链稳定性的重要性。与以前的研究不同，它提供了更广泛的评估，涵盖了广泛的常用生物链接成分，并检查了不同的灭菌方法对其稳定性的影响，从而为优化灭菌方案提供了新的见解，以增强和可复制的生物打印结果。

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引用次数: 0

Structural, mechanical and biomedical properties of 3D-printed Cu-doped Fe3O4/58S bioactive glass/polycaprolactone composite scaffold for bone tissue regeneration 3d打印cu掺杂Fe3O4/58S生物活性玻璃/聚己内酯复合骨组织再生支架的结构、力学和生物医学性能

Q1 Computer Science

Bioprinting

Pub Date : 2025-02-18 DOI: 10.1016/j.bprint.2025.e00400

Mojtaba Rajabinezhad , Mohammad Saeid Abbasi , Farnaz Heidari Laybidi , Mohammadjavad SharifianJazi , Mohammad Khodaei , Abbas Bahrami

This research investigates the mechanical, structural and biomedical implications of adding copper-doped magnetite nanoparticles, composited with 58S bioactive glass, to the 3D-printed polycaprolactone (PCL) scaffold. Cu-doped magnetite nanoparticles can be potentially used in hyperthermia and anti-bacterial applications. Additionally, the addition of bioactive glass was intended to promote bone tissue regeneration, hence creating a multi-purpose 3D-printed PCL scaffold. The PCL-nanocomposite mixtures were 3D printed using FDM method. Cu-doped magnetite nanoparticles-58S bioactive glass composite powders and 3D printed scaffolds were characterized using different techniques, including X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), Fourier-transform infrared spectroscopy (FTIR), and vibrating sample magnetometer (VSM). Cell viability, bioactivity, and anti-bacterial properties of scaffolds were also investigated. XRD/FESEM/FTIR results confirmed successful synthesis of Cu-doped magnetite nanoparticles-58S bioactive glass composite powder mixture with a perfect superparamagnetic behavior. Results also showed that the addition of secondary particles to the PCL is associated with some noticeable impacts on the wettability, roughness, and mechanical properties of printed scaffolds, with the best properties attained in the sample with 20 % of added secondary particles. Assessments of biomedical properties of printed specimens showed that the optimum printed scaffold has great anti-bacterial performance and promising cell viability and bioactivity, making is a great candidate for bone tissue engineering applications.

本研究探讨了在3d打印聚己内酯（PCL）支架中添加掺杂铜的磁铁矿纳米颗粒（由58S生物活性玻璃复合）的力学、结构和生物医学意义。铜掺杂磁铁矿纳米颗粒可以潜在地用于热疗和抗菌应用。此外，添加生物活性玻璃旨在促进骨组织再生，从而创建一个多用途的3d打印PCL支架。采用FDM方法对pcl -纳米复合材料进行3D打印。采用x射线衍射（XRD）、场发射扫描电镜（FESEM）、傅里叶变换红外光谱（FTIR）和振动样品磁强计（VSM）等不同技术对cu掺杂磁铁矿纳米颗粒- 58s生物活性玻璃复合粉末和3D打印支架进行了表征。研究了支架的细胞活力、生物活性和抗菌性能。XRD/FESEM/FTIR结果证实成功合成了cu掺杂磁铁矿纳米颗粒- 58s生物活性玻璃复合粉末混合物，具有完美的超顺磁性。结果还表明，在PCL中添加二次颗粒对打印支架的润湿性、粗糙度和力学性能有明显的影响，其中添加20%二次颗粒的样品的性能最好。生物医学性能的评估表明，最佳的打印支架具有良好的抗菌性能和良好的细胞活力和生物活性，是骨组织工程应用的理想候选材料。

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引用次数: 0

3D-printed PLA/Fe3O4/MgO hybrid composite scaffolds with improved properties 3d打印性能改善的PLA/Fe3O4/MgO杂化复合材料支架

Q1 Computer Science

Bioprinting

Pub Date : 2025-02-16 DOI: 10.1016/j.bprint.2025.e00398

Reyhaneh Ramezani , Reza Alizadeh , Sheyda Labbaf

Fused deposition modeling was successfully used to print porous scaffolds, using filaments of pure PLA, PLA/15 wt% Fe₃O₄ and PLA/15 wt% Fe₃O₄/5 wt% MgO. The magnetic, mechanical, thermal, and cellular properties of these samples were systematically evaluated and compared. The findings reveal that incorporating Fe₃O₄ enhances the magnetization saturation of PLA without compromising its mechanical and thermal integrity. Moreover, weight loss tests in phosphate-buffered saline solution indicated that the PLA/Fe₃O₄/MgO composite showed the highest degradation rate after 65 days. Biological assays confirmed enhanced cell adhesion and viability for the PLA/Fe₃O₄ and PLA/Fe₃O₄/MgO composites compared to pure PLA. These results demonstrate that the PLA/Fe₃O₄ and PLA/Fe₃O₄/MgO composites are promising alternatives of pure PLA for biomedical applications, addressing its inherent limitations, especially in cases where detection of implant by X-ray is required after implantation.

使用纯PLA、PLA/15 wt% Fe3O4和PLA/15 wt% Fe3O4/5 wt% MgO的长丝，熔融沉积模型成功地用于打印多孔支架。系统地评价和比较了这些样品的磁性、力学、热学和细胞性质。研究结果表明，加入Fe3O4可以提高PLA的磁化饱和度，但不会影响其机械和热完整性。此外，在磷酸盐缓冲盐水溶液中的失重试验表明，PLA/Fe3O4/MgO复合材料在65天后的降解率最高。生物实验证实，与纯PLA相比，PLA/Fe3O4和PLA/Fe3O4/MgO复合材料增强了细胞粘附性和活力。这些结果表明PLA/Fe3O4和PLA/Fe3O4/MgO复合材料是纯PLA在生物医学应用中的有希望的替代品，解决了其固有的局限性，特别是在植入后需要x射线检测植入物的情况下。

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引用次数: 0

FK506 binding protein like, FKBPL, as a novel therapeutic target in 2D and 3D bioprinted, models of cardiac fibrosis FK506结合蛋白样，FKBPL，在2D和3D生物打印心脏纤维化模型中作为新的治疗靶点

Q1 Computer Science

Bioprinting

Pub Date : 2025-02-04 DOI: 10.1016/j.bprint.2025.e00397

Michael Chhor , Shreya Barman , Fatemeh Heidari , Amy L. Bottomley , Tracy Robson , Kristine McGrath , Lana McClements

Background

Cardiac fibrosis characterised by increased collagen deposition and extracellular matrix (ECM) remodeling is one of the main causes of heart failure. Inflammation and hypoxia are key processes leading to cardiac fibrosis although the mechanisms are poorly understood. In this study, we developed an innovative 3D bioprinted model of cardiac fibrosis using tunable matrices. The role of an anti-angiogenic protein, FK506 binding protein like (FKBPL) was then elucidated, for the first time, using both 2D and 3D bioprinted, models of cardiac fibrosis.

Methods

3D bioprinted model of cardiac fibrosis was developed using fetal fibroblast cells (HFF08), customised ECM cardiac components and pro-fibrotic/hypoxic factors (TGF-β, 10 ng/ml, DMOG, 1 mM) ± FKBPL mimetic (AD-01, 100 mM). In parallel, 2D in vitro models were also employed.

Results

In the 3D bioprinted model, fibroblasts formed networks spontaneously, which were stimulated by all treatments (p < 0.05–0.0001). This was in conjunction with a trend towards reduced FKBPL expression, particularly in the presence of DMOG/AD-01 treatment. In 2D cell culture, AD-01 potentiated TGF-β-induced col1a1 (p < 0.0001) and mmp2 mRNA (p < 0.05) expression whereas DMOG or reduced FKBPL expression with AD-01 abrogated this (p < 0.05–0.001). Following siRNA FKBPL transfection, α-SMA was reduced (p < 0.05).

Conclusion

This 3D bioprinted model of cardiac fibrosis in conjunction with 2D cell models could be used for biomarker and drug therapy screening towards accelerating the development of treatments for this hard-to-treat condition. Low FKBPL expression could be protective in cardiac fibrosis through the reduction in collagen production and α-SMA expression, or TGF-β/HIF-1α-mediated effects. Therapeutic strategies that inhibit FKBPL should be explored to abrogate cardiac fibrosis.

以胶原沉积增加和细胞外基质（ECM）重塑为特征的心脏纤维化是心力衰竭的主要原因之一。炎症和缺氧是导致心脏纤维化的关键过程，尽管其机制尚不清楚。在这项研究中，我们开发了一种使用可调基质的创新型3D生物打印心脏纤维化模型。随后，利用2D和3D生物打印的心脏纤维化模型，首次阐明了抗血管生成蛋白FK506结合蛋白样（FKBPL）的作用。方法采用胎儿成纤维细胞（HFF08）、定制ECM心脏成分和促纤维化/缺氧因子（TGF-β, 10 ng/ml， DMOG, 1 mM）±FKBPL模拟物（AD-01, 100 mM）建立生物3d打印心脏纤维化模型。同时，还采用了2D体外模型。结果在生物3D打印模型中，成纤维细胞自发形成网络，所有处理均刺激成纤维细胞形成网络(p <；0.05 - -0.0001)。这与FKBPL表达降低的趋势相结合，特别是在DMOG/AD-01治疗的情况下。在2D细胞培养中，AD-01增强TGF-β诱导的col1a1 (p <；0.0001)和mmp2 mRNA (p <；而DMOG或AD-01降低FKBPL表达则消除了这一点(p <；0.05 - -0.001)。转染siRNA FKBPL后，α-SMA减少(p <；0.05)。结论：该3D生物打印心脏纤维化模型与2D细胞模型相结合，可用于生物标志物和药物治疗筛选，以加速这种难以治疗的疾病的治疗开发。低FKBPL表达可能通过减少胶原生成和α-SMA表达，或TGF-β/ hif -1α介导的作用，对心脏纤维化具有保护作用。应探索抑制FKBPL的治疗策略，以消除心脏纤维化。

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引用次数: 0

Modeling of oral squamous cell carcinoma microenvironment- A 3D bioprinting approach 口腔鳞状细胞癌微环境建模- 3D生物打印方法

Q1 Computer Science

Bioprinting

Pub Date : 2025-02-01 DOI: 10.1016/j.bprint.2024.e00381

Akhilanand Chaurasia , Gowri Sivaramakrishnan , Farah Asa’ad , Lena Larsson , Arwa Daghrery , Joana Marques , Francesca Spirito , Vitória Batista Clemente , Ana Carolina Morais Apolônio , Mahdieh Alipour , Rini Tiwari

Background

Oral squamous cell carcinoma (OSCC) presents significant challenges due to its complex microenvironment and invasive characteristics. Traditional two-dimensional (2D) culture systems are inadequate for modelling the intricate features of OSCC, necessitating advanced techniques for better in vitro modelling.

Objective

This review aims to explore the applications of 3D bioprinting in modelling the OSCC microenvironment, highlighting the advantages over conventional methods and discussing recent advancements in the field.

Methods

The review synthesizes recent literature on 3D bioprinting technologies, focusing on their application in replicating OSCC's microenvironment. Key areas include the integration of various cell types within a biomimetic extracellular matrix, the use of microfluidic systems to study tumor-stromal interactions, and the incorporation of advanced imaging modalities.

Results

3D bioprinting allows for the precise fabrication of complex OSCC tumor architectures, incorporating cancer cells, stromal cells, and immune cells. The integration of microfluidic systems facilitates the study of tumor invasion, metastasis, and drug response. Recent advancements in bioink development, particularly the use of patient-derived cells and biomolecules, enhance the physiological relevance of these models. Emerging imaging technologies provide unprecedented insights into the dynamics of OSCC progression within these constructs.

Conclusion

3D bioprinting shows immense potential for advancing the understanding of OSCC pathobiology and developing personalized therapeutic strategies. However, challenges such as standardizing bioink formulations and scaling fabrication techniques must be addressed to effectively translate these innovations into clinical practice.

口腔鳞状细胞癌（OSCC）因其复杂的微环境和侵袭性特征而面临重大挑战。传统的二维（2D）培养系统不足以模拟OSCC的复杂特征，需要先进的技术来更好地进行体外建模。目的探讨生物3D打印在OSCC微环境建模中的应用，强调其相对于传统方法的优势，并讨论该领域的最新进展。方法综述了近年来生物3D打印技术的研究进展，重点介绍了3D打印技术在复制OSCC微环境中的应用。关键领域包括在仿生细胞外基质中整合各种细胞类型，使用微流体系统研究肿瘤-基质相互作用，以及结合先进的成像方式。结果3d生物打印可以精确制造复杂的OSCC肿瘤结构，包括癌细胞、基质细胞和免疫细胞。微流控系统的集成促进了肿瘤侵袭、转移和药物反应的研究。生物连接发展的最新进展，特别是患者来源的细胞和生物分子的使用，增强了这些模型的生理学相关性。新兴的成像技术为OSCC在这些结构中的进展动态提供了前所未有的见解。结论3d生物打印技术在提高对OSCC病理生物学的认识和制定个性化治疗策略方面具有巨大的潜力。然而，必须解决诸如标准化生物链接配方和规模化制造技术等挑战，才能有效地将这些创新转化为临床实践。

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引用次数: 0

Zirconia-calcium silicate bioactive composites for dental applications using DLP additive manufacturing 使用DLP增材制造的牙科用氧化锆-硅酸钙生物活性复合材料

Q1 Computer Science

Bioprinting

Pub Date : 2025-02-01 DOI: 10.1016/j.bprint.2024.e00377

Ahmed Binobaid , Michele De Lisi , Josette Camilleri , Hany Hassanin , Khamis Essa

Zirconia has outstanding mechanical strength which made it a favourable material dental implants material. However, its use is limited by challenges in bone bonding and elasticity. This paper introduces a novel bioprinting ceramic material by mixing calcium silicate with zirconia to enhance bioactivity. Using the high precision and speed of Digital Light Processing (DLP), this study develops a novel zirconia-calcium silicate slurry for dental applications. The study reports the preparation of zirconia-calcium silicate, formulation of resin compositions, and optimization of the bioprinting, debinding and sintering. Employing a full factorial Design of Experiments (DOE), a systematic approach was implemented to identify optimal printing conditions such as the layer thickness, exposure time, and power. The results show that slurries formulated with BYK-111 as the dispersant and ACMO/PEGDA/TPO resin, coupled with 80 wt% solid loading, achieved the most favourable rheological properties, cure depth, and printing accuracy. The optimal printing conditions were 0.75 s exposure time, 300 % exposure power, and 30 μm layer thickness, ensured a relative density of the sintered implants exceeding 95 %. This study advances dental implant materials by introducing a novel DLP biomaterial with a slurry formulation, presenting significant implications for clinical applications and future research in developing advanced dental and medical implants.

氧化锆具有优异的机械强度，是一种良好的牙种植材料。然而，它的使用受到骨粘合和弹性方面的挑战的限制。介绍了一种新型的硅酸钙与氧化锆混合制备的生物打印陶瓷材料。利用数字光处理（DLP）的高精度和高速，本研究开发了一种新型的牙科应用的硅酸锆-钙浆料。本研究报道了硅酸锆钙材料的制备、树脂组成的配方以及生物打印、脱脂和烧结工艺的优化。采用全因子实验设计（DOE），采用系统的方法来确定最佳打印条件，如层厚度，曝光时间和功率。结果表明，以BYK-111为分散剂和ACMO/PEGDA/TPO树脂配制的浆料，加上80 wt%的固体负荷，获得了最佳的流变性能、固化深度和打印精度。最佳打印条件为曝光时间0.75 s，曝光功率300%，层厚30 μm，可确保烧结植入物的相对密度超过95%。本研究通过引入一种具有浆状配方的新型DLP生物材料，推动了牙科种植材料的发展，对临床应用和未来开发先进牙科和医疗种植材料的研究具有重要意义。

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引用次数: 0

4D printing in skin tissue engineering: A revolutionary approach to enhance wound healing and combat infections 皮肤组织工程中的4D打印：一种革命性的方法来增强伤口愈合和对抗感染

Q1 Computer Science

Bioprinting

Pub Date : 2025-02-01 DOI: 10.1016/j.bprint.2025.e00386

Laila A. Damiati , Samar A. Alsudir , Rean Y. Mohammed , Majed A. Majrashi , Shahad H. Albrahim , Aliyah algethami , Fatimah O. Alghamdi , Hala A. Alamari , Mai M. Alzaydi

Skin infection poses significant challenges in healthcare, demanding innovative solutions to enhance the efficacy of wound-repair interventions. 4D printing represents a revolutionary approach in addition to traditional wound-management strategies. 4D-printing materials, which are dynamic and responsive, can change their shape or properties over time in response to internal or external stimuli, creating a paradigm shift in how wounds are treated. This review explores the potential of 4D printing technology as a transformative solution addressing critical challenges in skin tissue engineering. It highlights the journey from 2D fabrication of skin implants to the current state of 4D printing focusing on skin tissue structures that allow for precise and sustained release of therapeutic agents while exhibiting self-healing properties. Also, the ability to integrate antimicrobials to the printed skin constructs that respond to specific stimuli, such as pH, light, temperature, humidity, or enzymes enables the on demand and controlled release of antimicrobial agents. Additionally, integrating artificial intelligence (AI) into the fabrication process of skin tissues represents a synergistic approach that combines advanced computational methodologies with biological principles to identify the optimal conditions for enhancing tissue regeneration. Indeed, 4D bioprinting and AI-driven precision in the customization of scaffolds based on patient-specific needs promise a new era of personalized medicine in skin tissue engineering.

皮肤感染对医疗保健提出了重大挑战，需要创新的解决方案来提高伤口修复干预措施的疗效。除了传统的伤口管理策略外，4D打印还代表了一种革命性的方法。3d打印材料具有动态和响应性，可以随着时间的推移改变其形状或特性，以响应内部或外部刺激，从而在伤口治疗方面产生范式转变。这篇综述探讨了4D打印技术作为解决皮肤组织工程关键挑战的变革性解决方案的潜力。它强调了从皮肤植入物的2D制造到4D打印的当前状态的旅程，重点是皮肤组织结构，允许治疗剂的精确和持续释放，同时表现出自我修复特性。此外，将抗菌剂整合到对特定刺激（如pH值、光、温度、湿度或酶）有反应的打印皮肤结构中的能力，使抗菌剂的按需和可控释放成为可能。此外，将人工智能（AI）集成到皮肤组织的制造过程中代表了一种协同方法，将先进的计算方法与生物学原理相结合，以确定增强组织再生的最佳条件。事实上，4D生物打印和基于患者特定需求的人工智能驱动的支架定制精度预示着皮肤组织工程个性化医疗的新时代。

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引用次数: 0

Advancing the in vitro drug screening models: Microbiome as a component of tissue-engineered skin 推进体外药物筛选模型：微生物组作为组织工程皮肤的组成部分

Q1 Computer Science

Bioprinting

Pub Date : 2025-02-01 DOI: 10.1016/j.bprint.2024.e00379

Vsevolod V. Shishkov , Polina Yu Bikmulina , Anna V. Kardosh , Sergey V. Tsibulnikov , Ekaterina V. Grekova , Yulia V. Kolesova , Polina A. Zakharova , Anastasiia M. Nesterova , Frederico David Alencar de Sena Pereira , Svetlana L. Kotova , Olga Yu Olisova , Massoud Vosough , Anastasia I. Shpichka , Peter S. Timashev

Currently, in vitro skin models are among the most advanced and frequently utilized tools in clinical practice and drug screening. The development of these models often involves the use of skin organoids and biofabrication techniques, such as 3D bioprinting. Despite this significant progress, the skin models employed in drug screening typically lack a microbiome component. Since the microbiome is recognized as a crucial element of healthy human skin, it is essential to integrate this aspect into existing skin models. This review outlines a pathway for the development of in vitro skin models that can be widely used as platforms for testing drugs and cosmetics. First, we discuss the diversity of the normal human microbiome and its interactions with human cells. Next, we examine current skin models, including those that incorporate microbiome components through various co-culturing methods. Finally, we discuss how biofabrication approaches can be combined with microbiome elements to create relevant and stable in vitro skin models.

目前，体外皮肤模型是临床实践和药物筛选中最先进、最常用的工具之一。这些模型的开发通常涉及使用皮肤类器官和生物制造技术，如3D生物打印。尽管取得了重大进展，但用于药物筛选的皮肤模型通常缺乏微生物组成分。由于微生物组被认为是健康人类皮肤的关键因素，因此将这方面整合到现有皮肤模型中至关重要。本文概述了体外皮肤模型的发展途径，该模型可广泛用于药物和化妆品的测试平台。首先，我们讨论了正常人类微生物组的多样性及其与人体细胞的相互作用。接下来，我们将研究当前的皮肤模型，包括那些通过各种共培养方法纳入微生物组成分的皮肤模型。最后，我们讨论了生物制造方法如何与微生物组元素相结合，以创建相关且稳定的体外皮肤模型。

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引用次数: 0