OpenNano最新文献_第8页

Microbicidal mechanisms for light-activated molecular nanomachines in Mycobacterium smegmatis: A model for pathogenic bacteria 耻垢分枝杆菌中光激活分子纳米机器的杀微生物机制：一种致病菌模型

Q2 Pharmacology, Toxicology and Pharmaceutics

OpenNano

Pub Date : 2025-02-25 DOI: 10.1016/j.onano.2025.100240

Thushara Galbadage , Dongdong Liu , James M. Tour , Jeffrey D. Cirillo , Richard S. Gunasekera

There is a global health crisis of antimicrobial resistance, responsible for over a million deaths annually. Mycobacterial infections are a major contributor to this crisis, causing more deaths than any other single infectious agent. Notably, the rise of multidrug-resistant (MDR), extensively drug-resistant (XDR), and totally drug-resistant (TDR) strains of Mycobacterium tuberculosis has led to higher mortality rates and challenge all existing antibiotic regimens. Light-activated molecular nanomachines (MNMs) represent a promising class of broad-spectrum antimicrobial agents that could help counter this rise in antimicrobial resistance. Addressing a key knowledge gap, this study explores the mechanisms of action for MNMs in Mycobacterium smegmatis, a surrogate model for pathogenic mycobacteria. We show that fast-rotor MNMs significantly reduce bacterial viability, achieving up to 97 % reduction in M. smegmatis with 30 minutes of light activation when compared to non-activated MNM 1 (p < 0.0001, t = 24.55), as determined by an unpaired t-test. Using fluorescence and confocal microscopy, we also show the colocalization of MNM 1 with M. smegmatis as part of their mechanism of action. The ability to translate these observations to pathogenic mycobacteria was demonstrated by the ability of MNM 1 to kill 93.5 % of M. tuberculosis with 5 minutes of light activation when compared to non-activated MNM 1 (p < 0.0001, t = 19.24). These findings suggest that MNMs have the potential to be innovative and sustainable antimicrobial agents for the treatment of pathogenic mycobacterial infections.

全球存在抗微生物药物耐药性的健康危机，每年造成100多万人死亡。分枝杆菌感染是造成这一危机的一个主要因素，它造成的死亡人数超过任何其他单一传染因子。值得注意的是，耐多药（MDR）、广泛耐药（XDR）和完全耐药（TDR）结核分枝杆菌菌株的增加导致了更高的死亡率，并对所有现有的抗生素治疗方案构成了挑战。光激活分子纳米机器（MNMs）代表了一类有前途的广谱抗菌剂，可以帮助对抗这种抗菌素耐药性的上升。为了解决一个关键的知识缺口，本研究探讨了MNMs在耻垢分枝杆菌（致病性分枝杆菌的替代模型）中的作用机制。我们发现，快速转子MNMs显著降低了细菌活力，与未激活的MNM 1相比，在30分钟的光激活下，耻垢分枝杆菌的活性降低了97% (p <；0.0001, t = 24.55)，由非配对t检验确定。利用荧光和共聚焦显微镜，我们还发现MNM - 1与耻垢分枝杆菌的共定位是其作用机制的一部分。与未激活的MNM 1相比，MNM 1在5分钟的光激活下杀死93.5%的结核分枝杆菌，证明了将这些观察结果转化为致病性分枝杆菌的能力(p <；0.0001, t = 19.24)。这些发现表明，MNMs有潜力成为治疗致病性分枝杆菌感染的创新和可持续的抗菌药物。

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引用次数: 0

Nanoformulation, characterization, and biological activity assays of extracts of Derris trifoliata Lour, a rutin-rich mangrove plant 一种富含芦丁的红树林植物——三叶草提取物的纳米配方、表征和生物活性分析

Q2 Pharmacology, Toxicology and Pharmaceutics

OpenNano

Pub Date : 2025-02-11 DOI: 10.1016/j.onano.2025.100236

Prattana Tanyapanyachon, Walailuk Chonniyom, Kananat Naksomboon, Jakarwan Yostawonkul, Paweena Dana, Udom Asawapirom, Nattika Saengkrit

Derris trifoliata Lour (DT) is a mangrove plant species with a promising anti-inflammatory effect. However, the application of DT as a topical anti-inflammatory agent is limited due to its poor bioavailability. Here, DT extract (DTE) from Kapoe District, Ranong Province, Thailand was selected due to its highest rutin over three investigated sources. The DTE was loaded into a nanoemulsion (NE), giving 74.33 ± 0.12 % and 75.29 ± 0.51 %, encapsulation efficiency of rutin and total phenolic content (TPC), respectively. The DTE-loaded NE (DTE-NE) exhibited a nano size (90.59 ± 1.27 nm) spherical, negative charge (-33.13 ± 0.12 mV), and narrow dispersity index (0.28 ± 0.01). The in vitro release profile of rutin and TPC from DTE-NE was slower compared to those from DTE. DTE-NE displayed a superior antioxidant capacity to DTE. Compared to DTE, DTE-NE treatment potentially lowered the inflammatory mediators (nitrite, interleukin-6, and tumor necrosis factor-alpha). Moreover, the physicochemical characteristics, antioxidant and anti-inflammatory activities remained unchanged after 1 month storage. In conclusion, the present study demonstrated the possible use of DTE-NE as a topical anti-inflammatory product.

trifoliata Lour （DT）是一种具有抗炎作用的红树植物。然而，由于其生物利用度差，DT作为局部抗炎药的应用受到限制。在这里，选择来自泰国拉廊省Kapoe地区的DT提取物（DTE），因为它在三个调查来源中含有最高的芦丁。将DTE包封在纳米乳（NE）中，对芦丁和总酚含量的包封率分别为74.33±0.12%和75.29±0.51%。dte负载的NE （DTE-NE）具有纳米尺寸（90.59±1.27 nm）球形，负电荷（-33.13±0.12 mV），分散性指数（0.28±0.01）窄。与DTE相比，DTE- ne的芦丁和TPC的体外释放速度较慢。DTE- ne表现出优于DTE的抗氧化能力。与DTE相比，DTE- ne治疗可能降低炎症介质（亚硝酸盐、白细胞介素-6和肿瘤坏死因子- α）。贮藏1个月后，其理化特性、抗氧化和抗炎活性基本保持不变。总之，本研究证明了DTE-NE作为局部抗炎产品的可能性。

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引用次数: 0

Resveratrol loaded zein nanoparticles for inhibiting proliferation of osteosarcoma cells: Synthesis, characterization, release profile, and cytotoxicity 白藜芦醇载玉米蛋白纳米颗粒抑制骨肉瘤细胞增殖：合成、表征、释放谱和细胞毒性

Q2 Pharmacology, Toxicology and Pharmaceutics

OpenNano

Pub Date : 2025-02-09 DOI: 10.1016/j.onano.2025.100237

Thanida Chuacharoen , Carlos E. Astete , Cristina M. Sabliov

Resveratrol loaded zein nanoparticles (Res ZNPs) were synthesized to deliver resveratrol to bone cells for inhibiting the proliferation of osteosarcoma cells. Zein chemically cross-linked with alendronate as a bone-targeting agent using EDC and NHS chemistry was also synthesized and the cross-linking was confirmed using Fourier-Transform Infrared and Nuclear Magnetic Resonance spectroscopy. Subsequently, Res ZNPs with and without alendronate were synthesized and characterized. The particles measured 273 to 294 nm with a narrow polydispersity index, and a zeta potential of -29 to -33 mV, respectively as evaluated by dynamic light scattering. The particles showed spherical morphology imaged by transmission electron microscopy and the entrapment efficiency and loading capacity were 63.0 % and 13.6 % for Res ZNPs and 69.1 % and 21.0 % for Res ZNPs with alendronate, respectively. Furthermore, the entrapped resveratrol of both systems was released in a three-phase manner under physiological condition (phosphate-buffered saline, PBS) at 37 °C over 24 h. Both systems exhibited suppression of osteosarcoma MG-63 cell proliferation and the inhibition rate was found slightly higher for targeted, Res ZNPs with alendronate. This research suggested that Res ZNPs conjugated with alendronate could be a candidate for effective bone-targeting delivery systems to inhibit the proliferation of osteosarcoma cells.

合成了白藜芦醇载玉米蛋白纳米颗粒（Res ZNPs），将白藜芦醇输送到骨细胞中，抑制骨肉瘤细胞的增殖。用EDC和NHS化学方法合成了玉米蛋白与阿仑膦酸钠作为骨靶向剂的化学交联，并用傅里叶变换红外光谱和核磁共振光谱证实了这种交联。随后，合成和表征了含和不含阿仑膦酸钠的Res ZNPs。动态光散射结果表明，纳米粒子的多分散性指数为273 ~ 294 nm， zeta电位为-29 ~ -33 mV。经透射电镜观察，颗粒形貌呈球形，阿仑膦酸盐对Res ZNPs的包封效率为63.0%、13.6%，对Res ZNPs的包封率为69.1%、21.0%。此外，在生理条件下（磷酸盐缓冲盐水，PBS） 37°C下，两种体系的白藜芦醇在24小时内以三相方式释放。两种体系都表现出对MG-63骨肉瘤细胞增殖的抑制作用，并且发现阿仑膦酸钠对靶向Res ZNPs的抑制率略高。本研究提示，Res ZNPs结合阿仑膦酸盐可作为一种有效的骨靶向递送系统，用于抑制骨肉瘤细胞的增殖。

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引用次数: 0

Osteogenic differentiation of mesenchymal stem cells in cell-laden culture of self-assembling peptide hydrogels 自组装肽水凝胶培养中间充质干细胞的成骨分化

Q2 Pharmacology, Toxicology and Pharmaceutics

OpenNano

Pub Date : 2025-01-28 DOI: 10.1016/j.onano.2025.100235

Faye Fouladgar , Robert Powell , Vishalakshi Irukuvarjula , Akhila Joy , Xiao Li , Neda Habibi

Mesenchymal stem cell (MSC) osteogenic differentiation requires scaffolds to support multiple stages of growth and differentiation signals. Fluorenyl-9-methoxycarbonyl diphenylalanine (Fmoc-FF) peptides self-assemble to create 3D nanofibers. Here, we cultured MSC in 2D and 3D Fmoc-FF layers to support their osteogenic differentiation. The stiffness of the hydrogels was tunable between 100 and 10,000 Pa which allows precise modulation of the cellular microenvironment. Scaffold stiffness impacted cell viability which softer scaffolds (100 Pa) favored higher viability. MSC formed spheroids in 3D hydrogel and showed spread morphology in 2D overlayers. Our results demonstrate that the Fmoc-FF 3D cultures significantly enhanced osteogenic differentiation, as evidenced by increased calcium deposition, elevated phosphatase activity, and the secretion of osteocalcin. We propose that the peptides provide integrin-binding sites that activate a cytoplasmic feedback loop essential for differentiation. These findings suggest that self-assembled Fmoc-FF peptide hydrogels, is a promising platform for bone tissue engineering applications.

间充质干细胞（MSC）的成骨分化需要支架来支持多阶段的生长和分化信号。氟酰-9-甲氧羰基二苯丙氨酸（Fmoc-FF）肽自组装创建三维纳米纤维。在这里，我们在2D和3D Fmoc-FF层中培养MSC以支持其成骨分化。水凝胶的硬度在100到10,000 Pa之间可调，从而可以精确调节细胞微环境。支架刚度影响细胞活力，较软的支架（100 Pa）有利于较高的活力。骨髓间充质干细胞在三维水凝胶中形成球形，在二维复层中呈扩散形态。我们的研究结果表明，Fmoc-FF 3D培养显著增强了成骨分化，钙沉积增加，磷酸酶活性升高，骨钙素分泌增加。我们提出肽提供整合素结合位点，激活分化所必需的细胞质反馈回路。这些发现表明，自组装Fmoc-FF肽水凝胶是一个很有前景的骨组织工程应用平台。

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引用次数: 0

Carrageenan bionanocomposite films incorporating Ag and Zn-Doped CeO₂ nanoparticles for active food packaging applications 含有Ag和zn掺杂CeO 2纳米粒子的卡拉胶生物复合膜用于活性食品包装

Q2 Pharmacology, Toxicology and Pharmaceutics

OpenNano

Pub Date : 2025-01-18 DOI: 10.1016/j.onano.2025.100234

Damar Rastri Adhika , Gita Genecya , Alvin Annayya Habibah , An Naas Amalia Rahardja Putri , Ubed Sonai Fahruddin Arrozi

The increasing demand for sustainable and safe food packaging has led to the exploration of bio-based materials and advanced packaging technologies. This study investigates the incorporation of silver (Ag) and zinc (Zn) doped cerium oxide nanoparticles (CeO₂ NPs) into carrageenan-based bionanocomposite films to enhance their antimicrobial properties, mechanical strength, and hydrophobicity. The synthesis of CeO₂ NPs, doped with varying concentrations of Ag and Zn, was achieved using the green synthesis method with green tea extract as a reducing agent. Characterization techniques such as X-ray diffraction (XRD), transmission electron microscopy (TEM), and Zeta potential analysis confirmed the successful doping and stability of the nanoparticles. The bionanocomposites were evaluated for their mechanical properties, water contact angle, and antibacterial activity against Escherichia coli and Bacillus cereus. Mechanical testing revealed that the addition of CeO₂ NPs, particularly Ag-doped CeO₂ NPs, significantly improved the tensile strength and Young's modulus of the bionanocomposites. Hydrophobicity assessments showed that Zn-doped CeO₂ NPs enhanced water resistance compared to Ag-doped CeO₂ NPs, making them more suitable for food packaging applications. Zn and Ag-doped CeO₂ NPs exhibited superior antibacterial activity compared to undoped CeO₂ NPs, with 20 wt% Ag-doped NPs showing the highest antibacterial activity compared to Amoxicillin as positive control and other variations. The study concludes that Zn and Ag-doped CeO₂ NPs are promising additives for developing effective and sustainable active food packaging materials.

对可持续和安全食品包装的需求日益增长，导致了对生物基材料和先进包装技术的探索。本研究研究了将银（Ag）和锌（Zn）掺杂的氧化铈纳米粒子（CeO₂NPs）掺入卡拉胶基生物纳米复合膜中，以提高其抗菌性能、机械强度和疏水性。以绿茶提取物为还原剂，采用绿色合成法合成了不同浓度Ag和Zn掺杂的CeO₂NPs。表征技术如x射线衍射（XRD）、透射电子显微镜（TEM）和Zeta电位分析证实了纳米颗粒的成功掺杂和稳定性。研究了生物纳米复合材料的力学性能、水接触角以及对大肠杆菌和蜡样芽孢杆菌的抗菌活性。力学测试表明，添加CeO₂NPs，特别是ag掺杂的CeO₂NPs，显著提高了生物纳米复合材料的抗拉强度和杨氏模量。疏水性评价表明，与掺银的CeO 2 NPs相比，掺锌的CeO 2 NPs的耐水性增强，使其更适合食品包装应用。与未掺杂的CeO₂NPs相比，Zn和ag掺杂的CeO₂NPs表现出更好的抗菌活性，其中20% ag掺杂的CeO₂NPs与阳性对照阿莫西林和其他变量相比表现出最高的抗菌活性。研究结果表明，锌和银掺杂的CeO₂NPs是开发有效和可持续的活性食品包装材料的有前途的添加剂。

{"title":"Carrageenan bionanocomposite films incorporating Ag and Zn-Doped CeO₂ nanoparticles for active food packaging applications","authors":"Damar Rastri Adhika , Gita Genecya , Alvin Annayya Habibah , An Naas Amalia Rahardja Putri , Ubed Sonai Fahruddin Arrozi","doi":"10.1016/j.onano.2025.100234","DOIUrl":"10.1016/j.onano.2025.100234","url":null,"abstract":"<div><div>The increasing demand for sustainable and safe food packaging has led to the exploration of bio-based materials and advanced packaging technologies. This study investigates the incorporation of silver (Ag) and zinc (Zn) doped cerium oxide nanoparticles (CeO₂ NPs) into carrageenan-based bionanocomposite films to enhance their antimicrobial properties, mechanical strength, and hydrophobicity. The synthesis of CeO₂ NPs, doped with varying concentrations of Ag and Zn, was achieved using the green synthesis method with green tea extract as a reducing agent. Characterization techniques such as X-ray diffraction (XRD), transmission electron microscopy (TEM), and Zeta potential analysis confirmed the successful doping and stability of the nanoparticles. The bionanocomposites were evaluated for their mechanical properties, water contact angle, and antibacterial activity against <em>Escherichia coli</em> and <em>Bacillus cereus</em>. Mechanical testing revealed that the addition of CeO₂ NPs, particularly Ag-doped CeO₂ NPs, significantly improved the tensile strength and Young's modulus of the bionanocomposites. Hydrophobicity assessments showed that Zn-doped CeO₂ NPs enhanced water resistance compared to Ag-doped CeO₂ NPs, making them more suitable for food packaging applications. Zn and Ag-doped CeO₂ NPs exhibited superior antibacterial activity compared to undoped CeO₂ NPs, with 20 wt% Ag-doped NPs showing the highest antibacterial activity compared to Amoxicillin as positive control and other variations. The study concludes that Zn and Ag-doped CeO₂ NPs are promising additives for developing effective and sustainable active food packaging materials.</div></div>","PeriodicalId":37785,"journal":{"name":"OpenNano","volume":"22 ","pages":"Article 100234"},"PeriodicalIF":0.0,"publicationDate":"2025-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143150133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Micro-channels array device fabricated via two photon lithography for cell migration studies in Neuroblastoma metastatic dissemination 双光子光刻微通道阵列装置在神经母细胞瘤转移传播中的细胞迁移研究

Q2 Pharmacology, Toxicology and Pharmaceutics

OpenNano

Pub Date : 2025-01-15 DOI: 10.1016/j.onano.2025.100233

Sara Micheli , Caterina Piunti , Elisa Varaschin , Marianna Peditto , Maria Luz Suarez , Marco Sorgato , Elisa Cimetta

Detailed studies of cells migration are key in understanding tumors metastatic spread. We used two-photon polymerization (2PP) to create precise microdevices for studying cell migration through micro-channels at a single cell resolution. Micro-channels are designed to mimic the structure of lymphatic vessels, conduits for cell movement in vivo. Neuroblastoma (NB) and human Mesenchymal Stem Cells (MSCs) represent the main tumor and its primary metastatic site. Our results revealed distinctive behaviors of NB and MSCs, both individually and in co-culture, hinting at a tumor-suppressive role of MSCs inhibiting NB migration. Pre-exposure of MSCs to NB-derived extracellular vesicles (EVs) significantly increased their motility towards tumor cells. Our platform more effectively replicates the in vivo environment of metastatic migration, with results providing new insights into the early dissemination of NB. Such microdevices hold great promise for advancing our understanding of metastasis and aiding the development of targeted anti-cancer therapies.

细胞迁移的详细研究是了解肿瘤转移扩散的关键。我们使用双光子聚合（2PP）来制造精确的微设备，用于在单细胞分辨率下研究细胞通过微通道的迁移。微通道是为了模拟淋巴管的结构而设计的，淋巴管是细胞在体内运动的管道。神经母细胞瘤（NB）和人间充质干细胞（MSCs）是主要的肿瘤及其原发性转移部位。我们的研究结果揭示了NB和MSCs在单独和共培养中的独特行为，暗示MSCs抑制NB迁移的肿瘤抑制作用。将MSCs预先暴露于nb来源的细胞外囊泡（ev）可显著增加其对肿瘤细胞的运动性。我们的平台更有效地复制了转移性迁移的体内环境，其结果为NB的早期传播提供了新的见解。这样的微型设备在促进我们对转移的理解和帮助靶向抗癌治疗的发展方面有着巨大的希望。

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引用次数: 0

Enhancing in Vitro anti-metastatic efficacy and deep penetration into tumor spheroid of docetaxel-loaded liposomes via size optimization for prostate cancer treatment 通过优化多西他赛脂质体在前列腺癌治疗中的大小，提高其体外抗转移效果及对肿瘤球体的深度渗透

Q2 Pharmacology, Toxicology and Pharmaceutics

OpenNano

Pub Date : 2025-01-04 DOI: 10.1016/j.onano.2024.100231

Saksorn Klibaim, Nutthanit Thumrongsiri, Natsorn Watcharadulyarat, Walailuk Chonniyom, Prattana Tanyapanyachon, Paweena Dana, Nattika Saengkrit

Castration-resistant prostate cancer (CRPC) presents a formidable challenge due to its aggressiveness and limited treatment options. Loading the drug docetaxel (DTX) into liposomes is a potential alternative approach to improve its efficacy. Several studies have reported that size optimization can improve drug efficacy in other cancer models. Therefore, this study explored the potential of size-optimization of docetaxel-loaded liposomes (LDTX) to improve in vitro efficacy against CRPC. The impacts of LDTX size (<100 nm and 100–200 nm) on cellular uptake, cytotoxicity in both monolayer and three-dimensional (3D) tumor spheroid models, and anti-metastatic effects were investigated. The results showed significant cellular internalization improvement with smaller LDTX, leading to better cytotoxicity in a monolayer cell culture than with larger LDTX. Moreover, smaller liposomes enabled deep penetration into the tumor spheroid, mimicking the tumor microenvironment and effectively eradicating cancer cells inside the spheroid. Interestingly, smaller liposomes also enhanced the anti-metastatic phenotype by inhibiting cancer cell invasion. The findings demonstrate that liposomes size is crucial in enhancing the efficacy of anti-cancer drugs. Therefore, size optimization is essential for developing highly effective formulations, requiring thorough investigation to identify the optimal liposomes size for specific applications.

去势抵抗性前列腺癌（CRPC）由于其侵袭性和有限的治疗选择而面临着巨大的挑战。将药物多西紫杉醇（DTX）装载到脂质体中是提高其疗效的潜在替代方法。有几项研究报道，尺寸优化可以提高其他癌症模型的药物疗效。因此，本研究探讨了多西他赛负载脂质体（LDTX）的尺寸优化潜力，以提高体外抗CRPC的疗效。研究了LDTX尺寸（100 nm和100 - 200 nm）对细胞摄取、单层和三维（3D）肿瘤球体模型的细胞毒性以及抗转移作用的影响。结果显示，较小的LDTX显著改善了细胞内化，导致单层细胞培养的细胞毒性优于较大的LDTX。此外，更小的脂质体能够深入肿瘤球体，模拟肿瘤微环境，有效地根除球体内的癌细胞。有趣的是，较小的脂质体也通过抑制癌细胞侵袭增强了抗转移表型。研究结果表明，脂质体的大小对提高抗癌药物的疗效至关重要。因此，尺寸优化对于开发高效配方至关重要，需要深入研究以确定特定应用的最佳脂质体尺寸。

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引用次数: 0

Evaluation of nano-sized virgin coconut oil (VCO)-loaded liposomes for enhancing mushroom and B16-F10 tyrosinase activity 纳米级初榨椰子油（VCO）脂质体增强蘑菇和B16-F10酪氨酸酶活性的研究

Q2 Pharmacology, Toxicology and Pharmaceutics

OpenNano

Pub Date : 2025-01-04 DOI: 10.1016/j.onano.2025.100232

Suwipa Ungphaiboon , Sutasinee Ardhanwanich , Duangkhae Maneenuan , Sirirat Pinsuwan , Pawika Mahasawat

This study evaluated the potential impact of VCO-loaded liposomes, particularly on the activation of tyrosinase. Optimized liposomes containing 1 % (w/w) VCO were prepared using the film deposition on carrier method, resulting in a particle size of 84.02 ± 5.00 nm and a zeta potential of -68.40 ± 2.78 mV. Encapsulation of VCO enhanced mushroom tyrosinase activity by 3-fold and exhibited lower cytotoxicity to B16-F10 cells compared to VCO alone. Moreover, a positive correlation was observed between the increase in intracellular tyrosinase activity and the concentrations of VCO (r = 0.8366) and VCO-loaded liposomes (r = 0.4794) in B16-F10 cells, while a negative correlation (r = -0.0545) was found for liposomes without VCO. A hair and eyebrow-darkening gel containing both VCO and VCO-loaded liposomes further enhanced mushroom tyrosinase activity by 283.33 ± 26.58 %. These findings suggest that VCO-loaded liposomes may serve as novel and effective nano-scale carriers for VCO in cosmetic applications.

本研究评估了载vco脂质体的潜在影响，特别是对酪氨酸酶的激活。采用载体膜沉积法制备了VCO含量为1% （w/w）的优化脂质体，其粒径为84.02±5.00 nm， zeta电位为-68.40±2.78 mV。与单独包封VCO相比，包封VCO可使蘑菇酪氨酸酶活性提高3倍，并且对B16-F10细胞的细胞毒性较低。此外，B16-F10细胞内酪氨酸酶活性的升高与VCO浓度（r = 0.8366）和负载VCO的脂质体浓度（r = 0.4794）呈正相关（r = -0.0545），而不含VCO的脂质体则呈负相关（r = -0.0545）。含有VCO和负载VCO脂体的头发和眉毛暗凝胶进一步提高了蘑菇酪氨酸酶活性283.33±26.58%。这些发现表明，负载VCO的脂质体可以作为VCO在化妆品应用中的新型和有效的纳米级载体。

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引用次数: 0

Q2 Pharmacology, Toxicology and Pharmaceutics

OpenNano

Pub Date : 2025-01-01

引用次数: 0

Q2 Pharmacology, Toxicology and Pharmaceutics

OpenNano

Pub Date : 2025-01-01

引用次数: 0