Sensing and Bio-Sensing Research最新文献_第8页

Facile synthesis of antimony oxide/nickel antimonate nanorods with highly-efficient sensing performance for Co ions detection 具有高效Co离子检测传感性能的氧化锑/锑酸镍纳米棒的简易合成

IF 4.9 Q1 CHEMISTRY, ANALYTICAL

Sensing and Bio-Sensing Research

Pub Date : 2025-10-09 DOI: 10.1016/j.sbsr.2025.100891

Chenxu Feng, Jiong Zhou, Jiamao Li, Xuewen Qiang, Zhengyu Cai, Lizhai Pei

The antimony oxide/nickel antimonate nanorods were prepared via a simple hydrothermal route using polyvinyl pyrrolidone (PVP) as the surfactant. The composite nanorods are composed of orthorhombic Sb₂O₃ and tetragonal NiSb₂O₆ phases, and have a length of longer than 1 μm, diameter of around 80 nm. The formation and growth of the antimony oxide/nickel antimonate nanorods were analyzed systematically by adjusting the PVP concentration, reaction temperature and reaction time. Glassy carbon electrode was modified by the antimony oxide/nickel antimonate nanorods for Co ions detection. There is an anodic peak at +0.61 V at the antimony oxide/nickel antimonate nanorods-modified electrode in 1 mM Co²⁺ solution with 0.1 M KCl. The optimum adsorption sites for Co adsorption on the antimony oxide/nickel antimonate nanorods were identified by density functional theory (DFT) calculation. The optimized pH, deposition time and potential, standing time for Co²⁺ detection are pH = 7, 120 s, −1.5 V and 40 s, respectively. The linear range and detection limit are 0.001–1000 μM and 0.12 nM, respectively. The antimony oxide/nickel antimonate nanorods-modified electrode demonstrates good sensing performance for detecting Co²⁺ which can potentially be applied to detect Co²⁺ ions in liquid environment.

以聚乙烯吡咯烷酮（PVP）为表面活性剂，采用简单的水热法制备了氧化锑/锑酸镍纳米棒。复合纳米棒由正交Sb2O3相和四方NiSb2O6相组成，长度大于1 μm，直径约为80 nm。通过调节PVP浓度、反应温度和反应时间，系统地分析了氧化锑/锑酸镍纳米棒的形成和生长。用氧化锑/锑酸镍纳米棒修饰玻碳电极，用于检测Co离子。在0.1 M KCl和1mm Co2+溶液中，氧化锑/锑酸镍纳米棒修饰电极在+0.61 V处有一个阳极峰。通过密度泛函理论（DFT）计算确定了氧化锑/锑酸镍纳米棒上Co的最佳吸附位点。Co2+检测的最佳pH值为7，沉积时间为120 s，电位、静置时间为- 1.5 V，静置时间为40 s。线性范围为0.001 ~ 1000 μM，检出限为0.12 nM。氧化锑/锑酸镍纳米棒修饰电极具有良好的Co2+检测性能，可用于液体环境中Co2+离子的检测。

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引用次数: 0

Smart dual-responsive poly(2-hydroxyethyl methacrylate)/phenylboronic acid systems for diabetes management: role of molecular weight in controlled insulin release 智能双响应聚（2-羟乙基甲基丙烯酸酯）/苯硼酸系统用于糖尿病管理：分子量在控制胰岛素释放中的作用

IF 4.9 Q1 CHEMISTRY, ANALYTICAL

Sensing and Bio-Sensing Research

Pub Date : 2025-10-09 DOI: 10.1016/j.sbsr.2025.100893

Fatemeh Ghashghaei Zadeh , Mahdi Salami Hosseini , Mehdi Salami-Kalajahi

In the present study, a dual pH- and glucose-responsive polymer based on poly(2-hydroxyethyl methacrylate) functionalized with 4-carboxyphenylboronic acid (p(HEMA-CPBA)) is successfully synthesized via reversible addition-fragmentation chain transfer (RAFT) polymerization at three different degrees of polymerization: 20, 40, and 200 with narrow dispersities (Đ) of 1.15, 1.17, and 1.18, respectively. The pK_a values of p(HEMA-CPBA) are measured ∼7.7, 7.9, and 8.0 using the titration method and 7.78, 7.97, and 8.17 using the spectral difference method. Glucose responsiveness is verified by particle size increase of the synthesized polymer upon increasing glucose concentration from 0 to 3 mg/mL due to glucose‑boronate complex formation. P(HEMA-CPBA) is further evaluated for insulin encapsulation efficiency to release it in response to glucose concentration. Insulin encapsulation efficiencies are 76.2, 69.7, and 58.7 % for HP20, HP40, and HP200 with corresponding loading capacities of 19.3, 17.5, and 14.6 %, respectively. In vitro release studies at pH 7.4 show glucose-dependent insulin release, with HP40 achieving up to 65 % cumulative release at 3 mg/mL glucose. The synthesized polymers show ∼90 % cell viability for HFF-2 cells, showing low toxicity. Fluorescence microscopy confirms preserved cell morphology showing p(HEMA-CPBA) as biocompatible platform for diabetic treatments.

在本研究中，以4-羧基苯基硼酸（p(HEMA-CPBA)）为功能化的聚（2-甲基丙烯酸羟乙酯）为基础，通过可逆加成-断裂链转移（RAFT）聚合，在3种不同聚合度下成功合成了pH-葡萄糖双响应聚合物：20、40和200，分散度（Đ）分别为1.15、1.17和1.18。用滴定法测得p（HEMA-CPBA）的pKa值为~ 7.7、7.9和8.0，用谱差法测得p（HEMA-CPBA）的pKa值为7.78、7.97和8.17。葡萄糖响应性通过葡萄糖-硼酸盐络合物的形成，当葡萄糖浓度从0增加到3 mg/mL时，合成聚合物的粒径增加来验证。进一步评估P（HEMA-CPBA）在葡萄糖浓度下释放胰岛素的包封效率。HP20、HP40和HP200的胰岛素包封效率分别为76.2、69.7和58.7%，相应的负载能力分别为19.3%、17.5%和14.6%。在pH 7.4下的体外释放研究显示葡萄糖依赖性胰岛素释放，HP40在3mg /mL葡萄糖下达到65%的累积释放。合成的聚合物对HFF-2细胞具有90%的细胞活力，具有低毒性。荧光显微镜证实保存的细胞形态显示p（HEMA-CPBA）是糖尿病治疗的生物相容性平台。

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引用次数: 0

A novel electrochemical sensor for in situ analysis of neurotransmitter profiles generated by induced pluripotent stem cell-derived neurons 一种新的电化学传感器，用于原位分析由诱导多能干细胞衍生的神经元产生的神经递质谱

IF 4.9 Q1 CHEMISTRY, ANALYTICAL

Sensing and Bio-Sensing Research

Pub Date : 2025-10-09 DOI: 10.1016/j.sbsr.2025.100894

Noha Shalabny , Mahmood Ali Saleh , Alexander Snezhko , Gad D. Vatine , Hadar Ben-Yoav

Neurons communicate through electrical signals and chemical messengers such as neurotransmitters (NTs). Disruptions between the action potential and the neurotransmitter release have been noted in disorders such as Parkinson's disease. However, monitoring the profiles of neurotransmitters released by neurons remains challenging. Electrochemical transduction methods provide powerful analytical tools for characterizing neurotransmitters; however, current electrochemical sensors work according to the lock-and-key approach and detect only single types of neurotransmitters, thus overlooking neurophysiological information from other neurotransmitters. Here, we present a novel holistic approach for in situ analysis of multiple redox-active neurotransmitters released by neurons. This approach is based on a high temporal resolution technique (fast-scan cyclic voltammetry; 8.5 ms transient readings) to record electrochemical signals generated by the neurotransmitters' profile using microelectrodes (100 μm in diameter). We recorded the electrochemical signals from motor neurons derived from induced pluripotent stem cells that were cultured on the microelectrode array. We recorded changes in the electrochemical signals generated by the neurons due to their chemical stimulation with potassium chloride (KCl; a chemical known to induce depolarization and enhance neuronal firing). The presence of KCl led to a significant increase in charge from 2320 ± 30 μC (no stimulation) to 2750 ± 70 μC and 3150 ± 64 μC with 30 mM and 90 mM KCl, respectively. These findings demonstrate our approach's potential for studying neurochemical communication and thereby advancing personalized therapies for neurological disorders. By enabling in situ neurotransmitter profiling from patient-derived cells, offering valuable insights into patient-specific diagnostics and treatment strategies.

神经元通过电信号和神经递质（nt）等化学信使进行交流。动作电位和神经递质释放之间的中断已经在帕金森病等疾病中被注意到。然而，监测神经元释放的神经递质的概况仍然具有挑战性。电化学转导方法为表征神经递质提供了强大的分析工具；然而，目前的电化学传感器是根据锁与钥匙的方法工作的，只能检测单一类型的神经递质，从而忽略了来自其他神经递质的神经生理信息。在这里，我们提出了一种新的整体方法，用于原位分析神经元释放的多种氧化还原活性神经递质。该方法基于高时间分辨率技术（快速扫描循环伏安法，8.5 ms瞬态读数），使用微电极（直径100 μm）记录神经递质谱产生的电化学信号。我们记录了在微电极阵列上培养的诱导多能干细胞产生的运动神经元的电化学信号。我们记录了由于氯化钾（KCl，一种已知能诱导去极化和增强神经元放电的化学物质）的化学刺激，神经元产生的电化学信号的变化。KCl的存在使植株的电荷量从2320±30 μC（无刺激）显著增加到2750±70 μC和3150±64 μC （KCl为30 mM和90 mM）。这些发现证明了我们的方法在研究神经化学交流方面的潜力，从而促进了神经系统疾病的个性化治疗。通过从患者来源的细胞中原位神经递质分析，为患者特异性诊断和治疗策略提供有价值的见解。

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引用次数: 0

In-vitro real-time magnetic recording of neuronal activity on spinal cord slices 脊髓切片上神经元活动的体外实时磁记录

IF 4.9 Q1 CHEMISTRY, ANALYTICAL

Sensing and Bio-Sensing Research

Pub Date : 2025-10-01 DOI: 10.1016/j.sbsr.2025.100885

Arturo Vera , Ivo Calaresu , Isidoro Martínez , Rubén Guerrero , Denis Scaini , Guillermo de Arana Schoebel , Jaime J. Hernandez , Isabel Rodríguez , Rodolfo Miranda , Eduardo Daniel Martín , Julio Camarero , María Teresa González , Lucas Pérez , Laura Ballerini

Recording the neural activity that originates from action potential dynamics has long been a major pursuit in neuroscience and, specifically, to develop neural interfaces, which are crucial for probing and understanding the nervous tissue. Conventional electrodes and emergent optical imaging (using genetically encoded fluorescence indicators) are complementary technologies to measure neuronal activity in-vivo but present intrinsic and general physical constraints. While optical imaging is difficult to translate in humans due to the strong genetic perturbations it involves, recordings through rigid implanted electrodes get frequently compromised over time by the foreign body reaction of the tissue that hinders the charge transfer to the electrode. In this scenario, magnetic sensing technologies can open further possibilities. Their working principle does not require intimate contact or charge transfer with the neural tissue and allows for well-tested soft polymeric coatings, which can facilitate the long-term functionality of implanted monitoring interfaces. Here, we report on the development of spintronic-based magnetic sensors able to detect neuronal activity emerging from spinal cord slices in physiological conditions at room temperature and with no magnetic shielding. We pharmacologically weaken synaptic inhibition inducing a switch from random to synchronous generation of action potentials, characterized by the appearance of slow-paced bursting in SCSs. The biological nature of the signals recorded was assessed by pharmacological removal of action potentials by tetrodotoxin and also by performing live Ca2+ imaging recordings simultaneously with magnetophysiology. Our results pave the way towards developing implanted devices that detect magnetic fields from neuronal activity for daily life applications.

长期以来，记录源自动作电位动力学的神经活动一直是神经科学的主要追求，特别是开发神经接口，这对于探测和理解神经组织至关重要。传统电极和新兴光学成像（使用基因编码荧光指示器）是测量体内神经元活动的互补技术，但存在内在和一般的物理限制。由于涉及到强烈的遗传扰动，光学成像很难在人体中进行翻译，通过刚性植入电极的记录经常会随着时间的推移而受到组织的异物反应的影响，这些反应会阻碍电荷转移到电极上。在这种情况下，磁传感技术可以开辟更多的可能性。它们的工作原理不需要与神经组织密切接触或电荷转移，并且允许经过良好测试的软聚合物涂层，这可以促进植入监测接口的长期功能。在这里，我们报告了基于自旋电子学的磁传感器的发展，该传感器能够在室温和无磁屏蔽的生理条件下检测脊髓切片中出现的神经元活动。我们在药理学上削弱突触抑制，诱导从随机到同步产生动作电位的转换，其特征是在scs中出现慢节奏的爆发。记录的信号的生物学性质是通过河豚毒素的药理学去除动作电位来评估的，同时也通过与磁生理学同时进行活Ca2+成像记录来评估。我们的研究结果为开发用于日常生活中检测神经元活动磁场的植入设备铺平了道路。

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引用次数: 0

A highly sensitive and selective one-dimensional Ag@hydrogen-bonded organic framework-based biosensor for the detection of the prohibited food additive ractopamine 一种用于检测违禁食品添加剂莱克多巴胺的高灵敏度和选择性一维Ag@hydrogen-bonded有机框架生物传感器

IF 4.9 Q1 CHEMISTRY, ANALYTICAL

Sensing and Bio-Sensing Research

Pub Date : 2025-09-30 DOI: 10.1016/j.sbsr.2025.100883

Mahmoud Roushani , Fatemeh Hamdi , Azam Zare Asadabadi , S. Jafar Hoseini

In this study, a novel and highly responsive biosensor was engineered for the identification of ractopamine (RAC), an illicit additive used to stimulate muscle development in farm animals. A hydrogen-bonded organic framework (HOF) was synthesized via a simple, economical, and environmentally friendly approach. To reinforce the electrochemical performance, silver nanoparticles (Ag NPs) were integrated into the HOF, thereby enlarging the electrode's active surface area and facilitating greater immobilization of RAC-specific aptamers (Apt). The successful immobilization of Apt RAC on the Ag@HOF-coated glassy carbon electrode (GCE) was validated using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). This sensing platform displayed a wide linear detection range from 0.01 fM - 0.25 nM, with an outstanding detection limit of 4 aM. Furthermore, the sensor demonstrated excellent specificity against potential interfering agents. Its reliable performance was also confirmed in complex biological samples, including milk and meat, indicating strong potential for practical, real-world applications.

在这项研究中，设计了一种新型的高响应生物传感器，用于识别莱克多巴胺（RAC），莱克多巴胺是一种用于刺激农场动物肌肉发育的非法添加剂。采用简单、经济、环保的方法合成了一种氢键有机骨架（HOF）。为了增强电化学性能，将银纳米粒子（Ag NPs）集成到HOF中，从而扩大电极的活性表面积，并促进rac特异性适配体（Apt）的固定化。利用循环伏安法（CV）和电化学阻抗谱法（EIS）验证了Apt RAC在Ag@HOF-coated玻碳电极（GCE）上的成功固定。该传感平台的线性检测范围为0.01 fM - 0.25 nM，检测限为4 aM。此外，该传感器对潜在干扰物表现出良好的特异性。在包括牛奶和肉类在内的复杂生物样品中也证实了其可靠的性能，表明其在实际应用中的强大潜力。

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引用次数: 0

Advances in the detection of Sortase A activity in Staphylococcus aureus 金黄色葡萄球菌中分类酶A活性检测的研究进展

IF 4.9 Q1 CHEMISTRY, ANALYTICAL

Sensing and Bio-Sensing Research

Pub Date : 2025-09-30 DOI: 10.1016/j.sbsr.2025.100886

Sujuan Sun , Junqing Yang , Xinya Han

Staphylococcus aureus (S. aureus) is one of the most common and important pathogenic bacteria and is the leading cause of hospital-acquired infections. Sortase A (SrtA), a cell surface-anchored transpeptidase in S. aureus, plays a critical role in the attachment of virulence-associated proteins to the cell wall. Given that SrtA is not directly involved in bacterial survival but mainly regulates pathogenicity, it has emerged as an attractive therapeutic target for developing anti-virulence strategies. Quantitative analysis of SrtA activity provides valuable insights into S. aureus colonization levels and virulence potential. Moreover, the detection method for SrtA facilitates the screening of inhibitors, and contributes to not only fundamental biological research but also pharmaceutical development and medical diagnostics. In this review, we discuss recent advances and modern techniques in novel methods for identifying SrtA activity, such as porous silicon resonant microcavities (pSiRM), magnetic nanoparticles, fluorescent proteins, and fluorescence resonance energy transfer (FRET)-based technologies. Additionally, we provide an objective evaluation of current biosensing technologies including high-performance liquid chromatography (HPLC), fluorescent, and electrochemical biosensors, with particular emphasis on their respective advantages and limitations in SrtA activity detection and inhibitor screening. This review aims to provide scientific evidence and potential strategies for developing new therapies against drug-resistant S. aureus while highlighting promising directions for next-generation anti-infective strategies.

金黄色葡萄球菌（金黄色葡萄球菌）是最常见和重要的致病菌之一，是医院获得性感染的主要原因。分类酶A （SrtA）是金黄色葡萄球菌细胞表面锚定的转肽酶，在毒力相关蛋白附着在细胞壁上起关键作用。由于SrtA不直接参与细菌存活，而主要调控致病性，因此它已成为开发抗毒策略的一个有吸引力的治疗靶点。SrtA活性的定量分析为金黄色葡萄球菌定植水平和毒力潜力提供了有价值的见解。此外，SrtA的检测方法有助于抑制剂的筛选，不仅有助于基础生物学研究，还有助于药物开发和医学诊断。在这篇综述中，我们讨论了鉴定SrtA活性的新方法的最新进展和现代技术，如多孔硅谐振微腔（pSiRM）、磁性纳米颗粒、荧光蛋白和基于荧光共振能量转移（FRET）的技术。此外，我们还客观评价了当前的生物传感技术，包括高效液相色谱（HPLC）、荧光和电化学生物传感器，特别强调了它们在SrtA活性检测和抑制剂筛选方面的各自优势和局限性。本文旨在为开发耐药金黄色葡萄球菌的新疗法提供科学依据和潜在策略，同时强调下一代抗感染策略的发展方向。

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引用次数: 0

Electrochemically pretreated graphite:glass thick films for ultra-trace detection of neonicotinoids in surface water and honey 电化学预处理石墨：用于地表水和蜂蜜中新烟碱超痕量检测的玻璃厚膜

IF 4.9 Q1 CHEMISTRY, ANALYTICAL

Sensing and Bio-Sensing Research

Pub Date : 2025-09-30 DOI: 10.1016/j.sbsr.2025.100890

Maksimiljan Dekleva , Barbara Repič , Danjela Kuscer , Nikola Tasić , Mitja Kolar , Helena Prosen , Gregor Marolt

Neonicotinoids (NNIs) pose a significant threat to pollinator populations and environmental health due to their widespread use and adverse effects on non-target organisms. Despite regulatory efforts, monitoring of NNIs in natural ecosystems remains challenging. In contrast to conventional chromatographic methods, integrated electrochemical sensors provide a rapid and on-site detection of NNIs. In this study, we report on a novel voltammetric sensor based on the electrochemical pretreatment of an in-house screen-printed graphite:glass electrode for a sensitive and selective detection of clothianidin (CLO), imidacloprid (IMI), and thiamethoxam (TMX). Electrochemical characterization via cyclic voltammetry and impedance spectroscopy demonstrated a significant enhancement in electrode performance after the pretreatment, offering strong adsorption properties and detection limits in the nanomolar level, achieving the lowest reported LODs for CLO (26 nmol L⁻¹) and IMI (12 nmol L⁻¹). For all three NNIs, two linear ranges between 0.5 and 40 μmol L⁻¹ were observed, alongside great sensitivity (8.1–13.1 μA μmol⁻¹ L, depending on the analyte). Notably, the recoveries obtained for NNIs in surface water and honey samples, ranging from 96.3 % to 107.8 %, indicate its applicability for real-sample and on-field monitoring. Utilizing one-step voltammetric electrode pretreatment and adsorption-based pre-concentration offers a cost-effective, fast, and ultra-sensitive platform, thus addressing a critical need in environmental analysis and pesticide regulation.

由于新烟碱类杀虫剂的广泛使用和对非目标生物的不良影响，对传粉媒介种群和环境健康构成了重大威胁。尽管做出了监管努力，但对自然生态系统中NNIs的监测仍然具有挑战性。与传统的色谱方法相比，集成电化学传感器提供了快速和现场检测NNIs。在这项研究中，我们报道了一种基于内部丝网印刷石墨玻璃电极电化学预处理的新型伏安传感器，用于灵敏和选择性地检测噻虫胺（CLO），吡虫啉（IMI）和噻虫嗪（TMX）。通过循环伏安法和阻抗谱进行的电化学表征表明，预处理后电极性能显著增强，具有很强的吸附性能和纳摩尔水平的检测限，对CLO （26 nmol L−1）和IMI （12 nmol L−1）的lod达到了报道的最低水平。对于所有三种NNIs，在0.5和40 μmol L−1之间观察到两个线性范围，同时具有很高的灵敏度（8.1-13.1 μA μmol−1 L，取决于分析物）。值得注意的是，NNIs在地表水和蜂蜜样品中的回收率为96.3%至107.8%，表明其适用于实际样品和现场监测。利用一步伏安电极预处理和基于吸附的预浓缩提供了一个经济、快速和超灵敏的平台，从而解决了环境分析和农药监管的关键需求。

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引用次数: 0

A high-performance yet cost-effective Cu/Ag bimetallic SPR biosensor augmented by halide perovskite integration: Unlocking ultra sensitivity and affordable urine-glucose sensing 卤化物钙钛矿集成增强的高性能且具有成本效益的Cu/Ag双金属SPR生物传感器：解锁超灵敏度和负担得起的尿糖传感

IF 4.9 Q1 CHEMISTRY, ANALYTICAL

Sensing and Bio-Sensing Research

Pub Date : 2025-09-30 DOI: 10.1016/j.sbsr.2025.100884

M. Mishra , A. Yadav , S. Senapati , S.K. Tripathy

Metal Halide Perovskite (MHP) is introduced into Surface Plasmon Resonance (SPR) biosensor with a bimetallic CuAg configuration and urine as the biosample. The Cu/Ag pairing, prior to the application of perovskite, achieves zero reflectivity at 67.8° with a sensitivity of 147°/RIU, exhibiting improvements of 130.8 % over Ag/Cu, 267.5 % over Cu alone, and 390 %, over Ag alone. The study decisively investigates four different halide perovskites—Cesium tin iodide (CsSnI₃), Formamidinium tin iodide (FASnI₃), Potassium tin iodide (KSnI₃), and Cesium lead iodide (CsPbI₃)—integrated into the Cu/Ag structure and optimizes their thicknesses as 8 nm, 10 nm, 12 nm, and 16 nm, respectively. These materials, known for their excellent optical properties and tunability, enhance light-matter interactions, leading to significant gains in SPR sensitivity and detection accuracy. The measured sensitivity values for these perovskites are 460, 406, 352, and 399°/RIU, respectively, with corresponding detection accuracies of 0.238, 0.294, 0.312, and 0.303 RIU⁻¹ with respective quality factors of 109.48, 119, 109.89, and 120. Evidently, CsSnI₃ is found to exhibit the highest sensitivity, supported by fair amount of detection accuracy and quality factor along with an estimated penetration depth of 150 nm, making it the most promising candidate for SPR performance enhancement in this study. Further, a layer of glucose oxidase enzyme is introduced over the halide layers which ensures the high selectivity of the proposed sensor.

将金属卤化物钙钛矿（MHP）以双金属CuAg结构引入表面等离子体共振（SPR）生物传感器中，以尿液为生物样品。在应用钙钛矿之前，Cu/Ag对在67.8°处达到零反射率，灵敏度为147°/RIU，比Ag/Cu提高130.8%，比Cu单独提高267.5%，比Ag单独提高390%。该研究决定性地研究了四种不同的卤化物钙钛矿——碘化锡铯（CsSnI₃）、碘化锡甲酰胺（FASnI₃）、碘化锡钾（KSnI₃）和碘化铅铯（CsPbI₃）——整合到Cu/Ag结构中，并优化了它们的厚度分别为8 nm、10 nm、12 nm和16 nm。这些材料以其优异的光学性能和可调性而闻名，增强了光与物质的相互作用，从而显著提高了SPR灵敏度和检测精度。这些钙钛矿的灵敏度分别为460、406、352和399°/RIU，检测精度分别为0.238、0.294、0.312和0.303 RIU−1，质量因子分别为109.48、119、109.89和120。显然，CsSnI₃被发现具有最高的灵敏度，得到相当数量的检测精度和质量因子的支持，并且估计穿透深度为150 nm，使其成为本研究中最有希望增强SPR性能的候选者。此外，在卤化物层上引入一层葡萄糖氧化酶，以确保所提出的传感器的高选择性。

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引用次数: 0

The role of S100B protein as a diagnostic biomarker for brain injury S100B蛋白作为脑损伤诊断生物标志物的作用

IF 4.9 Q1 CHEMISTRY, ANALYTICAL

Sensing and Bio-Sensing Research

Pub Date : 2025-09-30 DOI: 10.1016/j.sbsr.2025.100888

Nataliia Gnyliukh , James Wei , Winfried Neuhaus , Rabah Boukherroub , Sabine Szunerits

S100B is a brain protein, produced mainly by astrocytes, that indicates neurological injury by leaking into the bloodstream, cerebrospinal fluid (CSF), and urine. Elevated levels of S100B in blood and CSF serve as a marker for acute neural injury such as traumatic brain injury (TBI) and stroke. The extent of S100B elevation can help predict clinical outcomes after brain injury and monitor the effectiveness of treatment. Measuring S100B levels over time, or using a trajectory analysis, can provide more reliable information about injury progression and help predict secondary injuries. In order to predict clinical outcomes after brain injury, as well as to provide a basis for appropriate treatment and indicate treatment success, it is imperative to have appropriate analytical tools at hand. In this review, we focus on the research progress of S100B as an “alert” signalling molecule in the connection of brain injuries and critically assess current diagnostic assays for S100B, including Enzyme-Linked Immunosorbent Assay (ELISA) kits, biosensors, and point-of-care (PoC) devices.

S100B是一种主要由星形胶质细胞产生的脑蛋白，通过渗漏到血液、脑脊液和尿液中提示神经损伤。血液和脑脊液中S100B水平升高是急性神经损伤（如创伤性脑损伤（TBI）和中风）的标志。S100B升高的程度有助于预测脑损伤后的临床结局，监测治疗效果。随着时间的推移测量S100B水平，或使用轨迹分析，可以提供更可靠的损伤进展信息，并有助于预测继发性损伤。为了预测脑损伤后的临床结果，为适当的治疗提供依据，并提示治疗成功，掌握合适的分析工具是非常必要的。在这篇综述中，我们重点介绍了S100B作为脑损伤相关“警报”信号分子的研究进展，并批判性地评估了目前S100B的诊断方法，包括酶联免疫吸附试验（ELISA）试剂盒、生物传感器和护理点（PoC）设备。

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引用次数: 0

Ultrasensitive, label-free voltammetric detection of bis(2-ethylhexyl) phthalate based on poly-l-lysine/black phosphorus-porous graphene‑silver nanocomposite 基于聚赖氨酸/黑磷-多孔石墨烯-银纳米复合材料的超灵敏无标记邻苯二甲酸双（2-乙基己基）伏安检测

IF 4.9 Q1 CHEMISTRY, ANALYTICAL

Sensing and Bio-Sensing Research

Pub Date : 2025-09-30 DOI: 10.1016/j.sbsr.2025.100887

Chuanxiang Zhang , Jie Zhou , Shuo Li , Changchun Hu , Yimin Tan , Yan Deng

Di(2-ethylhexyl) phthalate (DEHP), a widely used plasticizer and known endocrine disruptor, has the potential to migrate through the food chain and accumulate in the human body, thereby posing significant risks to human health. Therefore, the accurate and timely detection of DEHP is of critical importance. A novel electrochemical molecularly imprinted sensor was developed based on poly-l-lysine/black phosphorus-porous graphene‑silver (PLL/BP-PG-Ag) nanocomposite for simple, rapid, highly sensitive and specific detection of trace di(2-ethylhexyl) phthalate (DEHP). The PLL/BP-PG-Ag nanocomposites, exhibiting excellent electrochemical properties, was synthesized through a simple solvothermal and ultrasonic method. The molecularly imprinted sensor (MIP/PLL/BP-PG-Ag/GCE) was fabricated via cyclic voltammetry electropolymerization using PLL/BP-PG-Ag as the substrate, o-phenylenediamine as the functional monomer, and DEHP as the template molecule. Under optimal experimental conditions, differential pulse voltammetry (DPV) analysis showed a wide linear range from 10fM to 2 μM, with a detection limit (LOD) of 7.09 fM and a quantification limit (LOQ) of 23.61 fM. The sensor also showed excellent selectivity when exposed to structurally similar interfering substances. The proposed MIP sensor was successfully applied to detect trace DEHP in cigarette packaging paper samples, yielding satisfactory recovery results.

邻苯二甲酸二（2-乙基己基）酯（DEHP）是一种广泛使用的增塑剂和已知的内分泌干扰物，有可能通过食物链迁移并在人体内积累，从而对人体健康构成重大风险。因此，准确、及时地检测DEHP至关重要。基于聚赖氨酸/黑磷-多孔石墨烯银（PLL/BP-PG-Ag）纳米复合材料，研制了一种新型电化学分子印迹传感器，用于简单、快速、高灵敏度和特异性检测痕量邻苯二甲酸二（2-乙基己基）酯（DEHP）。采用简单的溶剂热法和超声法合成了具有优异电化学性能的PLL/BP-PG-Ag纳米复合材料。以PLL/BP-PG-Ag为底物，邻苯二胺为功能单体，DEHP为模板分子，采用循环伏安电聚合法制备了分子印迹传感器（MIP/PLL/BP-PG-Ag/GCE）。在最佳实验条件下，差分脉冲伏安法（DPV）在10fM ~ 2 μM范围内线性范围宽，检出限（LOD）为7.09 fM，定量限（LOQ）为23.61 fM。当暴露于结构相似的干扰物质时，该传感器也表现出优异的选择性。该传感器成功地应用于香烟包装纸样品中痕量DEHP的检测，回收率令人满意。

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