Pub Date : 2019-04-28DOI: 10.3760/CMA.J.ISSN.1673-4181.2019.02.004
Wang Xiaohui, G. Ping, Lin Cheng, Bin Chen, Weiwei Liu, Zibo Yang, Meng-ni Shi
Objective To synthesize and characterize four acryl acetyl glucosamine (DA-NAG), and to determine its biocompatibility and cell membrane binding properties, so as to provide basis for its application in medical self agglutination gels. Methods DA-NAG was synthesized by esterification reaction. The products were characterized by mass spectrometry and hydrogen spectrum. Cytotoxicity test and subcutaneous implantation test were performed on the synthesized DA-NAG. The binding properties of DA-NAG to mouse fibroblast L929 cell membrane were detected using high performance liquid chromatography (HPLC). Results The characterization of mass spectrum and hydrogen spectrum are consistent with the characteristics of DA-NAG. The product has no cytotoxicity, and the subcutaneous implantation shows that the DA-NAG can be degraded at 4 weeks without obvious stimulation to the surrounding tissues. The result of HPLC shows the binding effect between the DA-NAG and cell membrane. Conclusions DA-NAG is successfully synthesized, and it has good cytocompatibility and binding ability to cell membrane. Key words: Cell membrane; Four acryl acetyl glucosamine; Self agglutination gel; Biosafety
{"title":"Synthesis and biosafety evaluation of four acryl acetyl glucosamine with cell membrane binding ability","authors":"Wang Xiaohui, G. Ping, Lin Cheng, Bin Chen, Weiwei Liu, Zibo Yang, Meng-ni Shi","doi":"10.3760/CMA.J.ISSN.1673-4181.2019.02.004","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1673-4181.2019.02.004","url":null,"abstract":"Objective \u0000To synthesize and characterize four acryl acetyl glucosamine (DA-NAG), and to determine its biocompatibility and cell membrane binding properties, so as to provide basis for its application in medical self agglutination gels. \u0000 \u0000 \u0000Methods \u0000DA-NAG was synthesized by esterification reaction. The products were characterized by mass spectrometry and hydrogen spectrum. Cytotoxicity test and subcutaneous implantation test were performed on the synthesized DA-NAG. The binding properties of DA-NAG to mouse fibroblast L929 cell membrane were detected using high performance liquid chromatography (HPLC). \u0000 \u0000 \u0000Results \u0000The characterization of mass spectrum and hydrogen spectrum are consistent with the characteristics of DA-NAG. The product has no cytotoxicity, and the subcutaneous implantation shows that the DA-NAG can be degraded at 4 weeks without obvious stimulation to the surrounding tissues. The result of HPLC shows the binding effect between the DA-NAG and cell membrane. \u0000 \u0000 \u0000Conclusions \u0000DA-NAG is successfully synthesized, and it has good cytocompatibility and binding ability to cell membrane. \u0000 \u0000 \u0000Key words: \u0000Cell membrane; Four acryl acetyl glucosamine; Self agglutination gel; Biosafety","PeriodicalId":61751,"journal":{"name":"国际生物医学工程杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47159356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-04-28DOI: 10.3760/CMA.J.ISSN.1673-4181.2019.02.010
Lei Zhang, Mubin Wei, Aifeng Liu, Shuwei Gong, Zhi-ping Tu
Objective To evaluate the efficacy of high tibial osteotomy(HTO) and unicompartmental knee arthroplasty(UKA) for knee unicompartmental osteoarthrits(KOA) using Meta analysis. Methods The controlled clinical trial literatures of HTO and UKA treating KOA were retrieved, the database including Chinese Biomedical Literature Database, Wanfang Data, CNKI, VIP Data, PubMed, Cochrane Library, EBSCO and Embase, and the search period was limited to the beginning of the database to July 2018. The literature was screened and evaluated, and Review Manager 5.3 software was used for Meta analysis. Results A total of 19 articles including 1 359 knee joints were included. Meta analysis results showed that HTO was superior to UKA in range of motion (ROM) (P 0.05). Conclusions Both HTO and UKA have their own advantages and disadvantages. It is necessary to properly choose the operation according to the patient's condition and psychological expectation. Key words: High tibal osteotomy; Unicompartmental knee arthroplasty; Unicompartmental knee osteoarthritis; Meta analysis
{"title":"Comparison of HTO and UKA in the treatment of unicompartmental knee osteoarthritis: a Meta analysis","authors":"Lei Zhang, Mubin Wei, Aifeng Liu, Shuwei Gong, Zhi-ping Tu","doi":"10.3760/CMA.J.ISSN.1673-4181.2019.02.010","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1673-4181.2019.02.010","url":null,"abstract":"Objective \u0000To evaluate the efficacy of high tibial osteotomy(HTO) and unicompartmental knee arthroplasty(UKA) for knee unicompartmental osteoarthrits(KOA) using Meta analysis. \u0000 \u0000 \u0000Methods \u0000The controlled clinical trial literatures of HTO and UKA treating KOA were retrieved, the database including Chinese Biomedical Literature Database, Wanfang Data, CNKI, VIP Data, PubMed, Cochrane Library, EBSCO and Embase, and the search period was limited to the beginning of the database to July 2018. The literature was screened and evaluated, and Review Manager 5.3 software was used for Meta analysis. \u0000 \u0000 \u0000Results \u0000A total of 19 articles including 1 359 knee joints were included. Meta analysis results showed that HTO was superior to UKA in range of motion (ROM) (P 0.05). \u0000 \u0000 \u0000Conclusions \u0000Both HTO and UKA have their own advantages and disadvantages. It is necessary to properly choose the operation according to the patient's condition and psychological expectation. \u0000 \u0000 \u0000Key words: \u0000High tibal osteotomy; Unicompartmental knee arthroplasty; Unicompartmental knee osteoarthritis; Meta analysis","PeriodicalId":61751,"journal":{"name":"国际生物医学工程杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48561397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-04-28DOI: 10.3760/CMA.J.ISSN.1673-4181.2019.02.003
Xuan Li, Chao-Meng Fan, Shu-xiang Lin, Wei Wang, Ping Wang, Yulian Fang, Hualei Cui
Objective To understand the infection status, epidemiological characteristics and genetic evolution of adenovirus in children with diarrhea in Tianjin. Methods A total of 1 609 fecal specimens were collected from hospitalized children with diarrhea from July 2017 to July 2018 in Tianjin Children's Hospital. Viral nucleic acid was extracted and amplified by the hexon gene fragment. Positive specimens were used for nucleic acid sequence determination and sequence alignment and phylogenetic tree analysis with known sequences in GenBank. Rotavirus in the stool specimens was detected by gold standard method, and campylobacter was detected by latex agglutination method. Bocavirus, Norovirus and Clostridium difficile were detected by quantitative real-time polymerase chain reaction (qRT-PCR). Results A total of 69 specimens with adenoviruses were detected in 1 609 cases, with a positive rate of 4.3%, in which 82.6% adenovirus-positive children were between 7 and 48 months old. The positive rate of adenovirus peaked in the summer and autumn, and the differences of this value among seasons were statistical significance (χ2=11.467, P=0.009). In these cases, the mixed infection rate of adenovirus was 26.1% (18/69), in which 7 cases were mixed with Norovirus, 7 cases were mixed with rotavirus and 4 cases were mixed with Clostridium difficile. A total of 39 adenovirus-positive PCR products were randomly selected for gene sequence detection, and 7 adenovirus genotypes were detected. Results showed that these viruses were mainly the type 41 enteric adenovirus (46.1%, 18/39), followed by type 31, type 3 and type 7 non-intestinal adenovirus, while types 1, 5 and 6 were relatively rare. Conclusions There are diverse types of adenovirus in children with diarrhea in Tianjin. The adenovirus-infected diarrhea mostly happen in summer and autumn, and is common in children. Key words: Diarrhea; Adenovirus infections, human; Molecular epidemiology; Genetic testing; Children
{"title":"Molecular epidemiological and genotypic analysis of human adenovirus infection in children with acute diarrhea in Tianjin from 2017 to 2018","authors":"Xuan Li, Chao-Meng Fan, Shu-xiang Lin, Wei Wang, Ping Wang, Yulian Fang, Hualei Cui","doi":"10.3760/CMA.J.ISSN.1673-4181.2019.02.003","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1673-4181.2019.02.003","url":null,"abstract":"Objective \u0000To understand the infection status, epidemiological characteristics and genetic evolution of adenovirus in children with diarrhea in Tianjin. \u0000 \u0000 \u0000Methods \u0000A total of 1 609 fecal specimens were collected from hospitalized children with diarrhea from July 2017 to July 2018 in Tianjin Children's Hospital. Viral nucleic acid was extracted and amplified by the hexon gene fragment. Positive specimens were used for nucleic acid sequence determination and sequence alignment and phylogenetic tree analysis with known sequences in GenBank. Rotavirus in the stool specimens was detected by gold standard method, and campylobacter was detected by latex agglutination method. Bocavirus, Norovirus and Clostridium difficile were detected by quantitative real-time polymerase chain reaction (qRT-PCR). \u0000 \u0000 \u0000Results \u0000A total of 69 specimens with adenoviruses were detected in 1 609 cases, with a positive rate of 4.3%, in which 82.6% adenovirus-positive children were between 7 and 48 months old. The positive rate of adenovirus peaked in the summer and autumn, and the differences of this value among seasons were statistical significance (χ2=11.467, P=0.009). In these cases, the mixed infection rate of adenovirus was 26.1% (18/69), in which 7 cases were mixed with Norovirus, 7 cases were mixed with rotavirus and 4 cases were mixed with Clostridium difficile. A total of 39 adenovirus-positive PCR products were randomly selected for gene sequence detection, and 7 adenovirus genotypes were detected. Results showed that these viruses were mainly the type 41 enteric adenovirus (46.1%, 18/39), followed by type 31, type 3 and type 7 non-intestinal adenovirus, while types 1, 5 and 6 were relatively rare. \u0000 \u0000 \u0000Conclusions \u0000There are diverse types of adenovirus in children with diarrhea in Tianjin. The adenovirus-infected diarrhea mostly happen in summer and autumn, and is common in children. \u0000 \u0000 \u0000Key words: \u0000Diarrhea; Adenovirus infections, human; Molecular epidemiology; Genetic testing; Children","PeriodicalId":61751,"journal":{"name":"国际生物医学工程杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45780821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-04-28DOI: 10.3760/CMA.J.ISSN.1673-4181.2019.02.012
Mengmeng Yang, Yan Wang, Liqing Du, Qiang Liu, Kaihua Ji
The small ubiquitin-like modified protein (SUMO) is a protein structurally similar to ubiquitin which is involved in post-translational modification of proteins. SUMOylation refers to the process that SUMO molecule covalently binding to the specific lysine site of target proteins through maturation, activation, binding and ligation by ubiquitin-like specific protease 1 (Ulp1), E1 activating enzyme, E2 binding enzyme, and E3 ligase. SUMOylation alters the activity of target proteins, which is involved in the regulation of various cellular functions such as transcriptional regulation, regulation of embryonic development, cellular stress, maintenance of chromatin structure and genomic stability. In recent years, it has been found that SUMOylation modification is also widely involved in DNA damage repair, especially DNA double-strand breaks (DSBs), which are the most serious types of DNA damage. SUMOylation is involved in almost all processes of DSBs repair, so its role in DNA damage repair has become a research hotspot. In this paper, the research progress of the regulation of SUMOylation in DSBs repair was reviewed. Key words: DNA damage; DNA repair; SUMOylation; DNA double-strand breaks; Non-homologous end joining; Homologous recombination
{"title":"Research progress of SUMOylation modification in DNA double-strand break repair","authors":"Mengmeng Yang, Yan Wang, Liqing Du, Qiang Liu, Kaihua Ji","doi":"10.3760/CMA.J.ISSN.1673-4181.2019.02.012","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1673-4181.2019.02.012","url":null,"abstract":"The small ubiquitin-like modified protein (SUMO) is a protein structurally similar to ubiquitin which is involved in post-translational modification of proteins. SUMOylation refers to the process that SUMO molecule covalently binding to the specific lysine site of target proteins through maturation, activation, binding and ligation by ubiquitin-like specific protease 1 (Ulp1), E1 activating enzyme, E2 binding enzyme, and E3 ligase. SUMOylation alters the activity of target proteins, which is involved in the regulation of various cellular functions such as transcriptional regulation, regulation of embryonic development, cellular stress, maintenance of chromatin structure and genomic stability. In recent years, it has been found that SUMOylation modification is also widely involved in DNA damage repair, especially DNA double-strand breaks (DSBs), which are the most serious types of DNA damage. SUMOylation is involved in almost all processes of DSBs repair, so its role in DNA damage repair has become a research hotspot. In this paper, the research progress of the regulation of SUMOylation in DSBs repair was reviewed. \u0000 \u0000 \u0000Key words: \u0000DNA damage; DNA repair; SUMOylation; DNA double-strand breaks; Non-homologous end joining; Homologous recombination","PeriodicalId":61751,"journal":{"name":"国际生物医学工程杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44970404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-04-28DOI: 10.3760/CMA.J.ISSN.1673-4181.2019.02.011
Kuo Li, You-jiu Zhang, Linlin Li, Cheng Li, Danqing Shen, Hu Rui
Objective To explore the feasibility of using the optimization parameters modification and optimization processes modification to reduce the total monitor units(MUs) in the Eclipse radiotherapy treatment planning system (TPS). Methods Based on the radiotherapy plan of 10 patients with nasopharyngeal carcinoma, a total of 90 plans were designed for 9 groups using different optimization parameters and processes. The total MUs and the exposure dose of the organs among the different plans were compared. Results There was no significant difference in the doses of the organs at risk (class I) under the premise of target dose requirements (all P>0.05). The increase of the weight of the target area and the organs at risk will increase the total MUs. The increase of the preset limit value of the minimum MUs in the subfield will reduce the total MUs. The increase of the fluence smoothness in the X and Y directions will increase the total MUs. An unreasonable minimum MU value will increase the total MUs. Under the condition that the organ exposure is not changed significantly, the influencing factors of MU are ranked as weight> fluence smoothness >minimum MUs. Conclusions Parameter setting and process planning can reduce the total MUs to a certain extent. However, due to the complexity of the influence of optimization parameters on the plan, the optimization process should be preferred. Especially in the Eclipse TPS, the method of gradual optimization to achieve the final dose distribution requirement and then remove the fluence re-optimization is more convenient and effective for reducing the total MUs. Key words: Eclipse TPS; Nasopharyngeal carcinoma; Fluence; Monitor units
{"title":"Feasibility method for reducing the total monitor units in Eclipse TPS","authors":"Kuo Li, You-jiu Zhang, Linlin Li, Cheng Li, Danqing Shen, Hu Rui","doi":"10.3760/CMA.J.ISSN.1673-4181.2019.02.011","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1673-4181.2019.02.011","url":null,"abstract":"Objective \u0000To explore the feasibility of using the optimization parameters modification and optimization processes modification to reduce the total monitor units(MUs) in the Eclipse radiotherapy treatment planning system (TPS). \u0000 \u0000 \u0000Methods \u0000Based on the radiotherapy plan of 10 patients with nasopharyngeal carcinoma, a total of 90 plans were designed for 9 groups using different optimization parameters and processes. The total MUs and the exposure dose of the organs among the different plans were compared. \u0000 \u0000 \u0000Results \u0000There was no significant difference in the doses of the organs at risk (class I) under the premise of target dose requirements (all P>0.05). The increase of the weight of the target area and the organs at risk will increase the total MUs. The increase of the preset limit value of the minimum MUs in the subfield will reduce the total MUs. The increase of the fluence smoothness in the X and Y directions will increase the total MUs. An unreasonable minimum MU value will increase the total MUs. Under the condition that the organ exposure is not changed significantly, the influencing factors of MU are ranked as weight> fluence smoothness >minimum MUs. \u0000 \u0000 \u0000Conclusions \u0000Parameter setting and process planning can reduce the total MUs to a certain extent. However, due to the complexity of the influence of optimization parameters on the plan, the optimization process should be preferred. Especially in the Eclipse TPS, the method of gradual optimization to achieve the final dose distribution requirement and then remove the fluence re-optimization is more convenient and effective for reducing the total MUs. \u0000 \u0000 \u0000Key words: \u0000Eclipse TPS; Nasopharyngeal carcinoma; Fluence; Monitor units","PeriodicalId":61751,"journal":{"name":"国际生物医学工程杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49460122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective To study and monitor the situation of femomaternal hemorrhage (FMH) in RhD-negative pregnant women in Tianjin, obtain the FMH data of such population, and analyze the relationship between FMH and age, blood type, gestational age, hemolytic disease of postpartum neonates, etc. Methods The FMH level was detected by flow cytometry with FITC-anti-HbF monoclonal antibody. The blood type was detected by blood serum method. The irregular antibody was identified by saline method and indirect anti-human ball method. The hemolysis of postpartum neonates was detected by three tests of hemolysis. Results The FMH volume of 86 RhD negative pregnant women was between 0 and 11.48 ml, with an average of 1.82 ml. There were 63.95% of pregnant women showed a volume of FMH 10 ml. The proportion of lower FMH in pregnant women ≤30 years old was >11.71% higher than that in the pregnant women >30 years old, but the difference was no statistical significant. There was no significant difference in FMH of pregnant women with O, A, B and AB types. The proportion of higher FMH in pregnant women with compatible ABO blood type with her husband was 12.46% lower than that of the heterozygous cases, but the difference was no statistical significant. The proportion of higher FMH in the pregnant women with 28 to 32 weeks gestational age was 14.55% higher than that of ≤28 weeks and was 35.32% higher than that of >32 weeks, and the differences were statistical significant. Three samples in the 86 samples were positive for anti-D antibody, and their three hemolytic test results were strongly positive with the anti-D titer from 1:2 to 1:32 and the FMH volume from 1.50 to 6.93 ml. The proportion of lower FMH in the 10 pregnant women without postpartum hemolysis was 70% higher than that in 5 pregnant women with postpartum hemolysis, but the differences were not statistical significant. Conclusions The results suggest that monitoring FMH content by flow cytometry can reflect FMH in Rh-negative pregnant women. The studies on the relationship between FMH and age, blood type, pregnant time and hemolytic disease of postpartum neonates can provide basically experimental data for standard use of anti-D immunoglobulin in pregnant women. Key words: RhD blood type; Fetomaternal hemorrhage; Anti-D immunoglobulin; Hemolytic disease of newborn
{"title":"Fetomaternal hemorrhage in RhD-negative pregnant women in Tianjin","authors":"Jing-hui Chong, Tong-Tong Li, Jinhui Xie, Xianghua Huang","doi":"10.3760/CMA.J.ISSN.1673-4181.2019.02.009","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1673-4181.2019.02.009","url":null,"abstract":"Objective \u0000To study and monitor the situation of femomaternal hemorrhage (FMH) in RhD-negative pregnant women in Tianjin, obtain the FMH data of such population, and analyze the relationship between FMH and age, blood type, gestational age, hemolytic disease of postpartum neonates, etc. \u0000 \u0000 \u0000Methods \u0000The FMH level was detected by flow cytometry with FITC-anti-HbF monoclonal antibody. The blood type was detected by blood serum method. The irregular antibody was identified by saline method and indirect anti-human ball method. The hemolysis of postpartum neonates was detected by three tests of hemolysis. \u0000 \u0000 \u0000Results \u0000The FMH volume of 86 RhD negative pregnant women was between 0 and 11.48 ml, with an average of 1.82 ml. There were 63.95% of pregnant women showed a volume of FMH 10 ml. The proportion of lower FMH in pregnant women ≤30 years old was >11.71% higher than that in the pregnant women >30 years old, but the difference was no statistical significant. There was no significant difference in FMH of pregnant women with O, A, B and AB types. The proportion of higher FMH in pregnant women with compatible ABO blood type with her husband was 12.46% lower than that of the heterozygous cases, but the difference was no statistical significant. The proportion of higher FMH in the pregnant women with 28 to 32 weeks gestational age was 14.55% higher than that of ≤28 weeks and was 35.32% higher than that of >32 weeks, and the differences were statistical significant. Three samples in the 86 samples were positive for anti-D antibody, and their three hemolytic test results were strongly positive with the anti-D titer from 1:2 to 1:32 and the FMH volume from 1.50 to 6.93 ml. The proportion of lower FMH in the 10 pregnant women without postpartum hemolysis was 70% higher than that in 5 pregnant women with postpartum hemolysis, but the differences were not statistical significant. \u0000 \u0000 \u0000Conclusions \u0000The results suggest that monitoring FMH content by flow cytometry can reflect FMH in Rh-negative pregnant women. The studies on the relationship between FMH and age, blood type, pregnant time and hemolytic disease of postpartum neonates can provide basically experimental data for standard use of anti-D immunoglobulin in pregnant women. \u0000 \u0000 \u0000Key words: \u0000RhD blood type; Fetomaternal hemorrhage; Anti-D immunoglobulin; Hemolytic disease of newborn","PeriodicalId":61751,"journal":{"name":"国际生物医学工程杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41340588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-04-28DOI: 10.3760/CMA.J.ISSN.1673-4181.2019.02.005
Luoping Chen, Lisha Zheng
Objective To investigate the role of Wnt/β-catenin signaling pathway in the osteogenic differentiation of human dental pulp stem cells (hDPSCs). Methods After 14 days of the calcium hydroxide training, the cytoskeletal changes of hDPSCs, the expression of β-catenin, i.e. the key promoter of in the Wnt signaling pathway, and the cell localization were detected by laser scanning confocal technique. The Wnt signaling pathways were up-regulated and inhibited, and the osteogenic differentiation and mineralization of hDPSCs were detected by Western Blot and alizarin red staining after 14 days of the training. Results The cytoskeleton of hDPSCs was rearranged by the effect of calcium hydroxide, and the β-catenin migration from nucleus to cytoplasm were observed. The number of calcium nodules in hDPSCs was decreased after blocking Wnt signaling pathway by Dickkopf-related protein 1 (DKK-1). The calcium hydroxide treatment can promoted dentin sialophosphoprotein (DSPP) expression in hDPSCs. Conclusions Calcium hydroxide can down-regulate the expression of canonical Wnt signaling pathway and promote osteogenic differentiation and mineralization and odontogenetic differentiation of hDPSCs. Key words: Calcium hydroxide; Osteogenic differentiation; Human dental pulp stem; Wnt pathway
{"title":"Regulation of Wnt signaling pathway on calcium hydroxide-promoted osteogenic differentiation of hDPSCs","authors":"Luoping Chen, Lisha Zheng","doi":"10.3760/CMA.J.ISSN.1673-4181.2019.02.005","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1673-4181.2019.02.005","url":null,"abstract":"Objective \u0000To investigate the role of Wnt/β-catenin signaling pathway in the osteogenic differentiation of human dental pulp stem cells (hDPSCs). \u0000 \u0000 \u0000Methods \u0000After 14 days of the calcium hydroxide training, the cytoskeletal changes of hDPSCs, the expression of β-catenin, i.e. the key promoter of in the Wnt signaling pathway, and the cell localization were detected by laser scanning confocal technique. The Wnt signaling pathways were up-regulated and inhibited, and the osteogenic differentiation and mineralization of hDPSCs were detected by Western Blot and alizarin red staining after 14 days of the training. \u0000 \u0000 \u0000Results \u0000The cytoskeleton of hDPSCs was rearranged by the effect of calcium hydroxide, and the β-catenin migration from nucleus to cytoplasm were observed. The number of calcium nodules in hDPSCs was decreased after blocking Wnt signaling pathway by Dickkopf-related protein 1 (DKK-1). The calcium hydroxide treatment can promoted dentin sialophosphoprotein (DSPP) expression in hDPSCs. \u0000 \u0000 \u0000Conclusions \u0000Calcium hydroxide can down-regulate the expression of canonical Wnt signaling pathway and promote osteogenic differentiation and mineralization and odontogenetic differentiation of hDPSCs. \u0000 \u0000 \u0000Key words: \u0000Calcium hydroxide; Osteogenic differentiation; Human dental pulp stem; Wnt pathway","PeriodicalId":61751,"journal":{"name":"国际生物医学工程杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48112340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-04-28DOI: 10.3760/CMA.J.ISSN.1673-4181.2019.02.014
Xiong Yang, Neng An, Rui Yang, Jie Li, Qi-duo Shi
Metformin is currently the first-line drug for the treatment of type 2 diabetes, and it is the most widely used oral hypoglycemic drug in the world. In addition to its significant hypoglycemic effect, metformin also has potential therapeutic effects on diseases such as tumors, obesity, thyroid diseases, polycystic ovarian diseases and cardiovascular diseases. However, the specific mechanism of action of metformin is still unclear. A large number of studies have shown that the regulation of energy metabolism by metformin is not only the core of its mechanism of action in the treatment of type 2 diabetes, but also an important mechanism for its therapeutic effects in diseases such as tumors and cardiovascular diseases. In this paper, the clinical application and related drug mechanisms of metformin in the treatment of non-diabetic diseases were summarized, so as to provide reference for the majority of medical workers, and look forward to a more in-depth exploration of its drug mechanism and clinical application in the future. Key words: Metformin; Tumor therapy; Mechanism; AMPK
{"title":"Application of metformin in the treatment of non-diabetic diseases","authors":"Xiong Yang, Neng An, Rui Yang, Jie Li, Qi-duo Shi","doi":"10.3760/CMA.J.ISSN.1673-4181.2019.02.014","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1673-4181.2019.02.014","url":null,"abstract":"Metformin is currently the first-line drug for the treatment of type 2 diabetes, and it is the most widely used oral hypoglycemic drug in the world. In addition to its significant hypoglycemic effect, metformin also has potential therapeutic effects on diseases such as tumors, obesity, thyroid diseases, polycystic ovarian diseases and cardiovascular diseases. However, the specific mechanism of action of metformin is still unclear. A large number of studies have shown that the regulation of energy metabolism by metformin is not only the core of its mechanism of action in the treatment of type 2 diabetes, but also an important mechanism for its therapeutic effects in diseases such as tumors and cardiovascular diseases. In this paper, the clinical application and related drug mechanisms of metformin in the treatment of non-diabetic diseases were summarized, so as to provide reference for the majority of medical workers, and look forward to a more in-depth exploration of its drug mechanism and clinical application in the future. \u0000 \u0000 \u0000Key words: \u0000Metformin; Tumor therapy; Mechanism; AMPK","PeriodicalId":61751,"journal":{"name":"国际生物医学工程杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42490028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-04-28DOI: 10.3760/CMA.J.ISSN.1673-4181.2019.02.013
Ying Zhang
From bacteria to mammalian cells, protein-DNA interactions are indispensable for all living organisms. In order to perform cell growth, differentiation, division, and regulation of cell function when stimulated by the external environment, DNA-encoded genetic information must be accurately transcribed, which is extremely important for the growth, development, and evolution of organisms. Manipulating the interaction of protein and DNA through biotechnology can modulate the expression of certain virulence genes, so as to contributing to the treatment of diseases. With the deepening of scientific research, more and more scientific technologies are applied in the field of protein and DNA interaction research. In this paper, the principles, advantages, disadvantages and applications of commonly used techniques in the study of protein-DNA interactions were reviewed, which will help researchers evaluate and select appropriate methods in their researches. Key words: Protein; DNA; Interaction; Detection technology
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Pub Date : 2019-03-26DOI: 10.11648/J.IJBECS.20180404.11
Awoke Giletew, Workaferahu Bekele
Primary dysmenorrhea is a painful menstrual cramp without any evident pathology to account for them. It is the most common gynecologic disorders and frequently observed cause of anxiety and discomfort among female adolescents. The aim of this study was to determine the prevalence of primary dysmenorrhea, its effect on academic performance and factors associated among Debre Tabor University students. An Institution based cross-sectional study was conducted between March and June, 2017. A total of 183 regular students from all departments at Debre Tabor University were included. Structured and pretested self-administrated questionnaires were used. Descriptive statistics and logistic regression analyses were employed. The result indicated the prevalence of primary dysmenorrhea was 62.3%. Among them students with primary dysmenorrhea 67 (58.7%) had negative effect on their daily activities. Family history of dysmenorrhea (AOR=2.930 95%CI: 1.505, 5.707), early age of menarche 12-15 (AOR=4.072, 95% CI. 2.039) and menstrual cycle irregularity (AOR=2.262, 95% CI: 1.102, 4,643) were factors that significantly contributed to the presence of primary dysmenorrhea. Prevalence of primary dysmenorrhea among Debre Tabor University student was high. It has negative effect on daily activities and academic performances of the students. Family history of dysmenorrhea, early age of menarche and menstrual cycle irregularity are factors that significantly contributed to the presence of primary dysmenorrhea among students of Debre Tabor University. Thus appropriate medical management and counseling should be considered to engage students in physical activity.
{"title":"Prevalence and Associated Factors of Primary Dysmenorrhea Among Debre Tabor University Students, North Central Ethiopia","authors":"Awoke Giletew, Workaferahu Bekele","doi":"10.11648/J.IJBECS.20180404.11","DOIUrl":"https://doi.org/10.11648/J.IJBECS.20180404.11","url":null,"abstract":"Primary dysmenorrhea is a painful menstrual cramp without any evident pathology to account for them. It is the most common gynecologic disorders and frequently observed cause of anxiety and discomfort among female adolescents. The aim of this study was to determine the prevalence of primary dysmenorrhea, its effect on academic performance and factors associated among Debre Tabor University students. An Institution based cross-sectional study was conducted between March and June, 2017. A total of 183 regular students from all departments at Debre Tabor University were included. Structured and pretested self-administrated questionnaires were used. Descriptive statistics and logistic regression analyses were employed. The result indicated the prevalence of primary dysmenorrhea was 62.3%. Among them students with primary dysmenorrhea 67 (58.7%) had negative effect on their daily activities. Family history of dysmenorrhea (AOR=2.930 95%CI: 1.505, 5.707), early age of menarche 12-15 (AOR=4.072, 95% CI. 2.039) and menstrual cycle irregularity (AOR=2.262, 95% CI: 1.102, 4,643) were factors that significantly contributed to the presence of primary dysmenorrhea. Prevalence of primary dysmenorrhea among Debre Tabor University student was high. It has negative effect on daily activities and academic performances of the students. Family history of dysmenorrhea, early age of menarche and menstrual cycle irregularity are factors that significantly contributed to the presence of primary dysmenorrhea among students of Debre Tabor University. Thus appropriate medical management and counseling should be considered to engage students in physical activity.","PeriodicalId":61751,"journal":{"name":"国际生物医学工程杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48033069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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