Pub Date : 2024-04-08DOI: 10.1007/s10404-024-02723-1
Mehrnaz Oveysi, Vahid Bazargan, Amir Nejat, Marco Marengo
This study introduces an innovative method aimed at achieving exceptional stability in emulsions. The primary focus is on re-emulsifying precisely controlled and uniform initial single emulsions, generated by microfluidic devices, to produce single-core double emulsions and core–shell microparticles. Departing from traditional approaches, our method employs a unique combination of advanced Two-level fractional factorial design and numerical simulation. These tools are utilized to discern and optimize critical parameters necessary for the formation of highly monodispersed stable single emulsions and their subsequent transformation into double emulsions. Correlations are established to estimate the size and stability of the primary single emulsion based on immiscible phase flow rate ratio and surfactant concentration. These correlations provide a comprehensive understanding that facilitates the intentional development of desired water-in-oil emulsions. The proposed microfluidic paradigm shows promise for the controlled and efficient production of single-core double emulsions, with broad applications in Pharmaceuticals, Food, and Cosmetics.
{"title":"Exploring the stability of single emulsion created by microfluidics and its use in the production of core–shell microparticles","authors":"Mehrnaz Oveysi, Vahid Bazargan, Amir Nejat, Marco Marengo","doi":"10.1007/s10404-024-02723-1","DOIUrl":"10.1007/s10404-024-02723-1","url":null,"abstract":"<div><p>This study introduces an innovative method aimed at achieving exceptional stability in emulsions. The primary focus is on re-emulsifying precisely controlled and uniform initial single emulsions, generated by microfluidic devices, to produce single-core double emulsions and core–shell microparticles. Departing from traditional approaches, our method employs a unique combination of advanced Two-level fractional factorial design and numerical simulation. These tools are utilized to discern and optimize critical parameters necessary for the formation of highly monodispersed stable single emulsions and their subsequent transformation into double emulsions. Correlations are established to estimate the size and stability of the primary single emulsion based on immiscible phase flow rate ratio and surfactant concentration. These correlations provide a comprehensive understanding that facilitates the intentional development of desired water-in-oil emulsions. The proposed microfluidic paradigm shows promise for the controlled and efficient production of single-core double emulsions, with broad applications in Pharmaceuticals, Food, and Cosmetics.</p></div>","PeriodicalId":706,"journal":{"name":"Microfluidics and Nanofluidics","volume":"28 5","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140571067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-04DOI: 10.1007/s10404-024-02726-y
Youngsik Song, Nafis Mustakim, Mayank Pandey, Sang-Woo Seo
We present the control of liquid flow through arrayed micron-sized pores in a macroporous silicon membrane. The pores are coated with about 150 nm polymer N-isopropylacrylamide (pNIPAAm) hydrogel brushes and 200 nm polypyrrole layer, which works as photothermal actuator. The size of pore openings is controlled by utilizing the swelling and de-swelling behavior of temperature-sensitive pNIPAAm brushes, and the temperature on pNIPAAm brushes is changed by 815 nm near infra-red (NIR) illumination to polypyrrole photothermal element layer. The dimension change of the pore openings is investigated by observing the transmitted light and fluorescence signal intensity through the pores in the membrane while changing the ambient temperature. It has shown that the intensity of transmitted light can be controlled by adjusting the ambient temperature across the low critical solution temperature (LCST) of the hydrogel brushes. The localized control of liquid flow through the pores is demonstrated by the diffusion of fluorescein dye from the bottom of the membrane to the surface of the membrane using pulsed NIR light illumination. Fast dynamic response of fluorescein dye diffusion upon the illumination of NIR light suggests that the presented photothermal actuation approach could be applied to diverse biomedical applications such as a localized drug release system.
{"title":"Localized flow control by photothermal actuation of pNIPAAm hydrogel brushes in a macroporous silicon membrane","authors":"Youngsik Song, Nafis Mustakim, Mayank Pandey, Sang-Woo Seo","doi":"10.1007/s10404-024-02726-y","DOIUrl":"10.1007/s10404-024-02726-y","url":null,"abstract":"<div><p>We present the control of liquid flow through arrayed micron-sized pores in a macroporous silicon membrane. The pores are coated with about 150 nm polymer N-isopropylacrylamide (pNIPAAm) hydrogel brushes and 200 nm polypyrrole layer, which works as photothermal actuator. The size of pore openings is controlled by utilizing the swelling and de-swelling behavior of temperature-sensitive pNIPAAm brushes, and the temperature on pNIPAAm brushes is changed by 815 nm near infra-red (NIR) illumination to polypyrrole photothermal element layer. The dimension change of the pore openings is investigated by observing the transmitted light and fluorescence signal intensity through the pores in the membrane while changing the ambient temperature. It has shown that the intensity of transmitted light can be controlled by adjusting the ambient temperature across the low critical solution temperature (LCST) of the hydrogel brushes. The localized control of liquid flow through the pores is demonstrated by the diffusion of fluorescein dye from the bottom of the membrane to the surface of the membrane using pulsed NIR light illumination. Fast dynamic response of fluorescein dye diffusion upon the illumination of NIR light suggests that the presented photothermal actuation approach could be applied to diverse biomedical applications such as a localized drug release system.</p></div>","PeriodicalId":706,"journal":{"name":"Microfluidics and Nanofluidics","volume":"28 5","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140570912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-02DOI: 10.1007/s10404-024-02724-0
Franciele Flores Vit, Yu Tzu Wu, Eric Fujiwara, Hernandes F. Carvalho, Lucimara Gaziola de la Torre
Some anticancer treatments may cause Multidrug Resistance (MDR). In these cases, cells pump the drug out of the intracellular environment, thereby preventing drug effects. Several strategies have been used to avoid MDR, including using two or more drugs at low concentrations to increase the sensitivity of cells to treatment. We present an effective, cheap, fast microfluidic alternative to test two drugs simultaneously using a reversible sealing and reusable device to determine the optimal concentration. We used the rugs doxorubicin (DOX) and paclitaxel (PXT) as proof of concept. The microdevice allows the testing of two drugs in real time. Furthermore, running two experiments in sextuplicates and control in the same microchip is possible. We used two combinations of drugs, varying the drug concentration (C1 = 0.010 mg.mL− 1 DOX and 0.002.mL− 1 mg PXT, C2 = 0.010 mg.mL− 1 DOX and 0.004 mg.mL− 1 PXT), and evaluated cell death over time. The intermediate drug concentrations were more efficient, reducing the time required to decrease the viability of breast tumor cells, MCF-7 (C1 = 180 and C2 = 120). In further analysis, the microdevice also allowed characterization of the effects of the drugs (antagonist, synergic, or additive). This microdevice is a reliable tool for estimating the different combinations of two drug concentrations in a single assay simply and quickly.
{"title":"Microfluidic chip for synergic drugs assay in 3D breast cancer cell","authors":"Franciele Flores Vit, Yu Tzu Wu, Eric Fujiwara, Hernandes F. Carvalho, Lucimara Gaziola de la Torre","doi":"10.1007/s10404-024-02724-0","DOIUrl":"10.1007/s10404-024-02724-0","url":null,"abstract":"<div><p>Some anticancer treatments may cause Multidrug Resistance (MDR). In these cases, cells pump the drug out of the intracellular environment, thereby preventing drug effects. Several strategies have been used to avoid MDR, including using two or more drugs at low concentrations to increase the sensitivity of cells to treatment. We present an effective, cheap, fast microfluidic alternative to test two drugs simultaneously using a reversible sealing and reusable device to determine the optimal concentration. We used the rugs doxorubicin (DOX) and paclitaxel (PXT) as proof of concept. The microdevice allows the testing of two drugs in real time. Furthermore, running two experiments in sextuplicates and control in the same microchip is possible. We used two combinations of drugs, varying the drug concentration (C<sub>1</sub> = 0.010 mg.mL<sup>− 1</sup> DOX and 0.002.mL<sup>− 1</sup> mg PXT, C<sub>2</sub> = 0.010 mg.mL<sup>− 1</sup> DOX and 0.004 mg.mL<sup>− 1</sup> PXT), and evaluated cell death over time. The intermediate drug concentrations were more efficient, reducing the time required to decrease the viability of breast tumor cells, MCF-7 (C<sub>1</sub> = 180 and C<sub>2</sub> = 120). In further analysis, the microdevice also allowed characterization of the effects of the drugs (antagonist, synergic, or additive). This microdevice is a reliable tool for estimating the different combinations of two drug concentrations in a single assay simply and quickly.</p></div>","PeriodicalId":706,"journal":{"name":"Microfluidics and Nanofluidics","volume":"28 5","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140571004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-29DOI: 10.1007/s10404-024-02720-4
Said Pashayev, Romain Lhermerout, Christophe Roblin, Eric Alibert, Jerome Barbat, Rudy Desgarceaux, Remi Jelinek, Edouard Chauveau, Saïd Tahir, Vincent Jourdain, Rasim Jabbarov, Francois Henn, Adrien Noury
Despite several decades of development, microfluidics lacks a sealing material that can be readily fabricated, leak-tight under high liquid water pressure, stable over a long time, and vacuum compatible. In this paper, we report the performances of a micro-scale processable sealing material for nanofluidic/microfluidics chip fabrication, which enables us to achieve all these requirements. We observed that micrometric walls made of SU-8 photoresist, whose thickness range from 35 to 135 µm, are at least leak-tight to 1.5 bars and up to 5.5 bars, exhibit no water porosity even after 2 months of aging, and are able to sustain under (10^{-5}) mbar vacuum. This sealing material is therefore reliable and versatile for building microchips, part of which must be isolated from liquid water under pressure or vacuum. Moreover, the fabrication process we propose does not require the use of either aggressive chemicals or high-temperature or high-energy plasma treatment. It thus opens a new perspective to seal microchips with sensitive surfaces containing nanomaterials.
{"title":"Quantifying the performances of SU-8 microfluidic devices: high liquid water tightness, long-term stability, and vacuum compatibility","authors":"Said Pashayev, Romain Lhermerout, Christophe Roblin, Eric Alibert, Jerome Barbat, Rudy Desgarceaux, Remi Jelinek, Edouard Chauveau, Saïd Tahir, Vincent Jourdain, Rasim Jabbarov, Francois Henn, Adrien Noury","doi":"10.1007/s10404-024-02720-4","DOIUrl":"10.1007/s10404-024-02720-4","url":null,"abstract":"<div><p>Despite several decades of development, microfluidics lacks a sealing material that can be readily fabricated, leak-tight under high liquid water pressure, stable over a long time, and vacuum compatible. In this paper, we report the performances of a micro-scale processable sealing material for nanofluidic/microfluidics chip fabrication, which enables us to achieve all these requirements. We observed that micrometric walls made of SU-8 photoresist, whose thickness range from 35 to 135 µm, are at least leak-tight to 1.5 bars and up to 5.5 bars, exhibit no water porosity even after 2 months of aging, and are able to sustain under <span>(10^{-5})</span> mbar vacuum. This sealing material is therefore reliable and versatile for building microchips, part of which must be isolated from liquid water under pressure or vacuum. Moreover, the fabrication process we propose does not require the use of either aggressive chemicals or high-temperature or high-energy plasma treatment. It thus opens a new perspective to seal microchips with sensitive surfaces containing nanomaterials.</p></div>","PeriodicalId":706,"journal":{"name":"Microfluidics and Nanofluidics","volume":"28 5","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140325418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-26DOI: 10.1007/s10404-024-02718-y
Ran Li, Zhaolin Gu, Zhang Li, Weizhen Lu, Guozhu Zhao, Junwei Su
Snap-off events are one of the most common and essential phenomena in two-phase flow in porous media. This paper uses the scanning results of a siltstone slice to construct a two-dimensional heterogeneous pore network structure to visualise microscopic snap-off phenomena and displacement processes accurately. The relationship between snap-off events and the non-wetting phase saturation was studied using two-phase flow displacement experiments. Results show that although the non-wetting phase snap-off events benefit freeing the trapped non-wetting phase in the microchannels, high-frequency snap-off events are the main reason for trapping the non-wetting phase during the displacement process, eventually leading to residuals. The frequency of non-wetting phase snap-off events in the pore network structure can be reduced to lower the non-wetting phase saturation and reduce the non-wetting phase residuals by increasing the displacement fluid viscosity, reducing the surface tension coefficient between the phases and increasing the flow rate.
{"title":"Behaviors of non-wetting phase snap-off events in two-phase flow: microscopic phenomena and macroscopic effects","authors":"Ran Li, Zhaolin Gu, Zhang Li, Weizhen Lu, Guozhu Zhao, Junwei Su","doi":"10.1007/s10404-024-02718-y","DOIUrl":"10.1007/s10404-024-02718-y","url":null,"abstract":"<div><p>Snap-off events are one of the most common and essential phenomena in two-phase flow in porous media. This paper uses the scanning results of a siltstone slice to construct a two-dimensional heterogeneous pore network structure to visualise microscopic snap-off phenomena and displacement processes accurately. The relationship between snap-off events and the non-wetting phase saturation was studied using two-phase flow displacement experiments. Results show that although the non-wetting phase snap-off events benefit freeing the trapped non-wetting phase in the microchannels, high-frequency snap-off events are the main reason for trapping the non-wetting phase during the displacement process, eventually leading to residuals. The frequency of non-wetting phase snap-off events in the pore network structure can be reduced to lower the non-wetting phase saturation and reduce the non-wetting phase residuals by increasing the displacement fluid viscosity, reducing the surface tension coefficient between the phases and increasing the flow rate.</p></div>","PeriodicalId":706,"journal":{"name":"Microfluidics and Nanofluidics","volume":"28 4","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140302330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-22DOI: 10.1007/s10404-024-02714-2
Jatin Panwar, Rahul Roy
In droplet microfluidic devices with suction-based flow control, the microchannel geometry and suction pressure at the outlet govern the dynamic properties of the two phases that influence the droplet generation. Therefore, it is critical to understand the role of geometry along with suction pressure in the dynamics of droplet generation to develop a predictive model. We conducted a comprehensive characterization of droplet generation in a flow focusing device with varying control parameters. We used these results to formulate a scaling argument and propose a governing parameter, called as modified capillary number (CaL), that combines normalized droplet volume with geometrical parameters (length of dispersed and continuous phase channels) and flow parameters (interfacial tension, phase viscosity and velocity) in a power law relationship. CaL effectively captures the transition from squeezing to dripping regimes of droplet generation, providing essential insights into the design requirements for suction-driven droplet generation. These findings are key to standardize microfluidic flow-focusing devices that can achieve the desired droplet generation behavior with optimal pressure consumption.
{"title":"Modified capillary number to standardize droplet generation in suction-driven microfluidics","authors":"Jatin Panwar, Rahul Roy","doi":"10.1007/s10404-024-02714-2","DOIUrl":"10.1007/s10404-024-02714-2","url":null,"abstract":"<div><p>In droplet microfluidic devices with suction-based flow control, the microchannel geometry and suction pressure at the outlet govern the dynamic properties of the two phases that influence the droplet generation. Therefore, it is critical to understand the role of geometry along with suction pressure in the dynamics of droplet generation to develop a predictive model. We conducted a comprehensive characterization of droplet generation in a flow focusing device with varying control parameters. We used these results to formulate a scaling argument and propose a governing parameter, called as modified capillary number (Ca<sub>L</sub>), that combines normalized droplet volume with geometrical parameters (length of dispersed and continuous phase channels) and flow parameters (interfacial tension, phase viscosity and velocity) in a power law relationship. Ca<sub>L</sub> effectively captures the transition from squeezing to dripping regimes of droplet generation, providing essential insights into the design requirements for suction-driven droplet generation. These findings are key to standardize microfluidic flow-focusing devices that can achieve the desired droplet generation behavior with optimal pressure consumption.</p></div>","PeriodicalId":706,"journal":{"name":"Microfluidics and Nanofluidics","volume":"28 4","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140204062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-20DOI: 10.1007/s10404-024-02719-x
Shahin Mohammad Nejad, Frank A. Peters, Silvia V. Nedea, Arjan J. H. Frijns, David M. J. Smeulders
In rarefied gas dynamics scattering kernels deserve special attention since they contain all the essential information about the effects of physical and chemical properties of the gas–solid surface interface on the gas scattering process. However, to study the impact of the gas–surface interactions on the large-scale behavior of fluid flows, these scattering kernels need to be integrated in larger-scale models like Direct Simulation Monte Carlo (DSMC). In this work, the Gaussian mixture (GM) model, an unsupervised machine learning approach, is utilized to establish a scattering kernel for monoatomic (Ar) and diatomic ((hbox {H}_{2})) gases directly from Molecular Dynamics (MD) simulations data. The GM scattering kernel is coupled to a pure DSMC solver to study isothermal and non-isothermal rarefied gas flows in a system with two parallel walls. To fully examine the coupling mechanism between the GM scattering kernel and the DSMC approach, a one-to-one correspondence between MD and DSMC particles is considered here. Benchmarked by MD results, the performance of the GM-DSMC is assessed against the Cercignani–Lampis–Lord (CLL) kernel incorporated into DSMC simulation (CLL-DSMC). The comparison of various physical and stochastic parameters shows the better performance of the GM-DSMC approach. Especially for the diatomic system, the GM-DSMC outperforms the CLL-DSMC approach. The fundamental superiority of the GM-DSMC approach confirms its potential as a multi-scale simulation approach for accurately measuring flow field properties in systems with highly nonequilibrium conditions.
{"title":"A hybrid Gaussian mixture/DSMC approach to study the Fourier thermal problem","authors":"Shahin Mohammad Nejad, Frank A. Peters, Silvia V. Nedea, Arjan J. H. Frijns, David M. J. Smeulders","doi":"10.1007/s10404-024-02719-x","DOIUrl":"10.1007/s10404-024-02719-x","url":null,"abstract":"<div><p>In rarefied gas dynamics scattering kernels deserve special attention since they contain all the essential information about the effects of physical and chemical properties of the gas–solid surface interface on the gas scattering process. However, to study the impact of the gas–surface interactions on the large-scale behavior of fluid flows, these scattering kernels need to be integrated in larger-scale models like Direct Simulation Monte Carlo (DSMC). In this work, the Gaussian mixture (GM) model, an unsupervised machine learning approach, is utilized to establish a scattering kernel for monoatomic (Ar) and diatomic (<span>(hbox {H}_{2})</span>) gases directly from Molecular Dynamics (MD) simulations data. The GM scattering kernel is coupled to a pure DSMC solver to study isothermal and non-isothermal rarefied gas flows in a system with two parallel walls. To fully examine the coupling mechanism between the GM scattering kernel and the DSMC approach, a one-to-one correspondence between MD and DSMC particles is considered here. Benchmarked by MD results, the performance of the GM-DSMC is assessed against the Cercignani–Lampis–Lord (CLL) kernel incorporated into DSMC simulation (CLL-DSMC). The comparison of various physical and stochastic parameters shows the better performance of the GM-DSMC approach. Especially for the diatomic system, the GM-DSMC outperforms the CLL-DSMC approach. The fundamental superiority of the GM-DSMC approach confirms its potential as a multi-scale simulation approach for accurately measuring flow field properties in systems with highly nonequilibrium conditions.</p></div>","PeriodicalId":706,"journal":{"name":"Microfluidics and Nanofluidics","volume":"28 4","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10404-024-02719-x.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140204105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The microfluidic system is capable of recapitulating key attributes of in vivo circumstances and, therefore, becomes a valuable platform for better understanding tumor growth dynamics and evaluating drug efficiency. While numerical simulations have been envisioned as powerful tools for validating versatile performance of advanced microfluidic platforms, cell growth within these microchannels has not yet been theoretically modeled. In this paper, we developed an experimental data-driven cellular automaton model, which was adopted for simulating cell behaviors and drug responses in a microfluidic system. The boundaries of the cellular automata lattices and prohibited zones for simulation were directly converted from microscopic images of cell morphology and the microchamber configuration. The dynamic progression of tumor growth at the avascular stage was predicted by incorporating the biophysical and molecular characteristics of cells and their interactions with surrounding environment. The simulated proliferation rate of tumor cells over time demonstrated its dependency on nutrient delivery, aligning well with experimental observations in the microfluidic culture. The spatiotemporal efficacy of the chemotherapeutic compound doxorubicin (DOX) on the microfluidic culture was also simulated. The similarity between in silico simulations and in vitro tumor response upon drug interaction highlighted the potential of the computational models as complementary tools for predicting the drug treatment efficacy with acceptable accuracy before practical applications.
{"title":"Simulation of avascular tumor growth and drug response in a microfluidic device with a cellular automaton model","authors":"Sijia Liu, Yuewu Li, Chunxiao Chen, Zhiyu Qian, Hongjun Wang, Yamin Yang","doi":"10.1007/s10404-024-02717-z","DOIUrl":"10.1007/s10404-024-02717-z","url":null,"abstract":"<div><p>The microfluidic system is capable of recapitulating key attributes of in vivo circumstances and, therefore, becomes a valuable platform for better understanding tumor growth dynamics and evaluating drug efficiency. While numerical simulations have been envisioned as powerful tools for validating versatile performance of advanced microfluidic platforms, cell growth within these microchannels has not yet been theoretically modeled. In this paper, we developed an experimental data-driven cellular automaton model, which was adopted for simulating cell behaviors and drug responses in a microfluidic system. The boundaries of the cellular automata lattices and prohibited zones for simulation were directly converted from microscopic images of cell morphology and the microchamber configuration. The dynamic progression of tumor growth at the avascular stage was predicted by incorporating the biophysical and molecular characteristics of cells and their interactions with surrounding environment. The simulated proliferation rate of tumor cells over time demonstrated its dependency on nutrient delivery, aligning well with experimental observations in the microfluidic culture. The spatiotemporal efficacy of the chemotherapeutic compound doxorubicin (DOX) on the microfluidic culture was also simulated. The similarity between in silico simulations and in vitro tumor response upon drug interaction highlighted the potential of the computational models as complementary tools for predicting the drug treatment efficacy with acceptable accuracy before practical applications.</p></div>","PeriodicalId":706,"journal":{"name":"Microfluidics and Nanofluidics","volume":"28 4","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140155819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
To enhance focusing performance, we proposed an integrated microchannel with expansion–contraction arrays (ECA) on the inner wall of the curved microchannel (CIECA) and compared it with a straight microchannel with ECA (SECA) as well as the traditional integrated microchannel of ECA on the outer wall of the curved channel (COECA). We investigated the particle-focusing mechanisms in these microchannels through a combination of experiments and numerical simulations. The proposed integrated microchannel demonstrates significant improvements in focusing performance compared to SECA and COECA, which is attributed to its consistent Dean flow. In contrast, COECA shows the poorest performance because of inconsistent Dean flow. The focusing width in the proposed integrated microchannel is reduced to 1/3 of that in COECA and 1/2 of that in SECA. Furthermore, the focusing performance of CIECA improves as the Reynolds number increases, eventually forming a single trajectory when the Reynolds number (at contraction) reaches 83.33. Finally, the impact of particle size on focusing performance was investigated through numerical simulations. The focusing performance of the CIECA is the best in these three microchannels. In CIECA, as the particle size increases, the focusing width initially decreases and then increases. Among them, 8 and 10 μm particles can achieve complete focusing. This study serves as a crucial reference for comprehending and enhancing particle focusing through the synergy of multi-Dean flow.
{"title":"Curved microchannels with inner wall expansion–contraction array for particle focusing","authors":"Ruihan Zhuang, Kaixin Song, Zhibin Wang, Gang Chen, Ying Chen, Lisi Jia","doi":"10.1007/s10404-024-02715-1","DOIUrl":"10.1007/s10404-024-02715-1","url":null,"abstract":"<div><p>To enhance focusing performance, we proposed an integrated microchannel with expansion–contraction arrays (ECA) on the inner wall of the curved microchannel (CIECA) and compared it with a straight microchannel with ECA (SECA) as well as the traditional integrated microchannel of ECA on the outer wall of the curved channel (COECA). We investigated the particle-focusing mechanisms in these microchannels through a combination of experiments and numerical simulations. The proposed integrated microchannel demonstrates significant improvements in focusing performance compared to SECA and COECA, which is attributed to its consistent Dean flow. In contrast, COECA shows the poorest performance because of inconsistent Dean flow. The focusing width in the proposed integrated microchannel is reduced to 1/3 of that in COECA and 1/2 of that in SECA. Furthermore, the focusing performance of CIECA improves as the Reynolds number increases, eventually forming a single trajectory when the Reynolds number (at contraction) reaches 83.33. Finally, the impact of particle size on focusing performance was investigated through numerical simulations. The focusing performance of the CIECA is the best in these three microchannels. In CIECA, as the particle size increases, the focusing width initially decreases and then increases. Among them, 8 and 10 μm particles can achieve complete focusing. This study serves as a crucial reference for comprehending and enhancing particle focusing through the synergy of multi-Dean flow.</p></div>","PeriodicalId":706,"journal":{"name":"Microfluidics and Nanofluidics","volume":"28 4","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140155683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-09DOI: 10.1007/s10404-024-02708-0
Victor Bradley Bednar, Kenichi Takahata
Pulsed thermal energy causes piecewise actuation of a nitinol cantilever providing the mechanical force required to evacuate a chamber constructed of parylene C. This proof-of-principle micropump demonstrates an alternative to typical evacuation and rectification methods utilized in most micropumps. The chamber and normally closed channels that serve as valves are all of parylene C construction, leading to the flexibility of the device. The nitinol cantilever functions as an actuator capable of yielding successive partial chamber evacuations until achieving complete evacuation. Piecewise shape recovery of the actuator was made viable by implementing a Peltier device, providing the means for supplying responsive and controlled thermal energy. Experiments delivered measurements of consecutive advancement of shape recovery using a laser displacement sensor while monitoring the temperature with fiber-optic sensors. The release of a saturated lithium chloride solution from the pump was monitored by observing conductivity changes in the experimental area. Theoretically predicting a release amount used calculations for the expected recovery of the actuator based on displacement characterization via a logistic curve fit against actuator temperature data. The measured release amounts correlated well with the theoretically predicted values made using the temperature values obtained near the device during the release. These works provide novel approaches to micropump fabrication and implementation and new strategies for predicting the recovery of shape memory alloys. The micropump concepts are viable in many fields, such as biomedical applications: in vivo drug delivery, organ-on-chip, and lab-on-chip devices, to name a few. Likewise, a simple prediction for nitinol recovery has vast potential.
脉冲热能使镍钛诺悬臂片状启动,从而产生抽真空所需的机械力,将一个由对二甲苯 C 制成的腔室抽空。作为阀门的腔体和常闭通道全部采用对二甲苯 C 结构,从而提高了设备的灵活性。镍钛诺悬臂作为致动器,能够连续产生部分腔室排空,直至实现完全排空。通过采用珀尔帖(Peltier)装置,使推杆的片状形状恢复成为可能,从而提供了反应灵敏且可控的热能。实验使用激光位移传感器测量形状恢复的连续进展,同时使用光纤传感器监测温度。通过观察实验区域的电导率变化,监测泵中饱和氯化锂溶液的释放情况。在理论上预测释放量时,使用的是基于位移特征的推杆预期恢复计算方法,通过与推杆温度数据进行对数曲线拟合。测量到的释放量与利用释放过程中在装置附近获得的温度值进行的理论预测值相关性很好。这些工作为微型泵的制造和实施提供了新方法,也为预测形状记忆合金的恢复提供了新策略。微泵概念在许多领域都是可行的,例如生物医学应用:体内给药、芯片上的器官和芯片上的实验室设备等等。同样,镍钛诺恢复的简单预测也具有巨大潜力。
{"title":"A thermally actuated biocompatible flexible micropump for surface adaptable mounting","authors":"Victor Bradley Bednar, Kenichi Takahata","doi":"10.1007/s10404-024-02708-0","DOIUrl":"10.1007/s10404-024-02708-0","url":null,"abstract":"<div><p>Pulsed thermal energy causes piecewise actuation of a nitinol cantilever providing the mechanical force required to evacuate a chamber constructed of parylene C. This proof-of-principle micropump demonstrates an alternative to typical evacuation and rectification methods utilized in most micropumps. The chamber and normally closed channels that serve as valves are all of parylene C construction, leading to the flexibility of the device. The nitinol cantilever functions as an actuator capable of yielding successive partial chamber evacuations until achieving complete evacuation. Piecewise shape recovery of the actuator was made viable by implementing a Peltier device, providing the means for supplying responsive and controlled thermal energy. Experiments delivered measurements of consecutive advancement of shape recovery using a laser displacement sensor while monitoring the temperature with fiber-optic sensors. The release of a saturated lithium chloride solution from the pump was monitored by observing conductivity changes in the experimental area. Theoretically predicting a release amount used calculations for the expected recovery of the actuator based on displacement characterization via a logistic curve fit against actuator temperature data. The measured release amounts correlated well with the theoretically predicted values made using the temperature values obtained near the device during the release. These works provide novel approaches to micropump fabrication and implementation and new strategies for predicting the recovery of shape memory alloys. The micropump concepts are viable in many fields, such as biomedical applications: in vivo drug delivery, organ-on-chip, and lab-on-chip devices, to name a few. Likewise, a simple prediction for nitinol recovery has vast potential.</p></div>","PeriodicalId":706,"journal":{"name":"Microfluidics and Nanofluidics","volume":"28 4","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140097288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}