The widespread use of retreaded tires has led to great concern about their uncertainty in terms of atmospheric pollution because their degraded tribological performance leads to emit more tire wear particles (TWPs) into the environment. This paper presented the features of TWPs from retreaded tire rubber via a self-developed rolling contact test rig. Normalized comparison, emission per unit worn mass and per kilometer were employed to compare the influence of the test parameters both on emission and on environmental impact quantitatively between the new tire rubber (N-TR) and tire rubber mixed with reclaimed rubber (RR-TR). The results showed that heavy load could induce retreaded tire emitting TWPs3.0 and 5.0 a maximum of 12–19% higher than N-TR. Meanwhile, N-TR could emit TWPs3.0 1.2 times greater than RR-TR would when the same mass was worn, implying that N-TR could be 1.2 times more dangerous than RR10-TR to the public respiratory health, which, however, must be treated dialectically and critically. Furthermore, in the same travel distance, the risk extent of retreaded tire to environmental pollution could enhance 7–9% than that of new tire according to the TWPs amount. Additionally, an interesting and practical comparison shown that negative filler, e.g., reclaimed rubber, which had inactive impacts on anti-wear performance, could induce more individual TWPs with pitted, and bumpy surfaces rather than uneven-surfaced TWPs with small bulges governed by positive filler, e.g., carbon nanotubes. Superior molecular-chain mobility and stronger interfacial adhesion in retreaded tires are the dominant mechanisms responsible for their higher TWPs emission compared with new tires. This study provides a scientific basis for improving and minimizing the emission of the TWPs emission from retreaded tire, balancing the cost economy and the air quality based on the contributions.