D. Onan-Martinez , M.A.T. de Bari , H. Olmo , J. Lance , I.M. Toledo , J.M. Tricarico , G.E. Dahl
Methane is a major GHG produced by cattle. Strategies to reduce enteric methane include supplementation with monensin, which has shown variable outcomes. Therefore, we hypothesized that monensin (Rumensin, Elanco, Greenfield, IN) would reduce enteric CH4 yield and intensity in lactating dairy cows (∼207 DIM). Twenty cows were enrolled in a 2 4-wk period crossover completely randomized design with repeated measures including 2 treatments: control (CON, n = 10/period) and monensin (MON, n = 10/period). Monensin treatment consisted of 300 mg of monensin in 34 g/cow of dried distillers grains (DDG) whereas CON was DDG alone, both top-dressed daily on the TMR. Cows were milked 3 times/d and housed in a sand-bedded freestall barn with Calan gates to collect individual DMI. A GreenFeed system was used to measure enteric gas output. Daily milk yield and DMI were measured from each cow during the study. Additionally, fat, protein and lactose percentages were measured weekly in milk. Average (±SEM) DMI (MON = 25.4 ± 0.3, CON = 25.4 ± 0.3, kg/d) and ECM (MON = 35.8 ± 1.09, CON = 36.4 ± 1.09; kg/d) were similar between treatments. Conversely, daily CH4 was significantly reduced with monensin (MON = 207.1 ± 13.1, CON = 257.2 ± 13.1 g/d). Methane yield showed a significant decline with treatment (MON = 8.1 ± 0.4, CON = 9.9 ± 0.4 g/kg DMI). Last, methane intensity relative to ECM tended to decline with monensin (MON = 5.7 ± 0.3, CON = 6.5 ± 0.3 g/kg of ECM). The results suggest that monensin reduces daily enteric CH4 emissions and yield in dairy cattle, providing evidence to support its use to reduce CH4 emissions from cattle.
甲烷是牛产生的一种主要温室气体。减少肠道甲烷的策略包括补充莫能菌素,这显示出不同的结果。因此,我们假设莫能菌素(rumenin, Elanco, Greenfield, IN)会降低泌乳奶牛肠道CH4的产量和强度(~ 207 DIM)。选取20头奶牛,采用24周交叉完全随机设计,重复试验包括2个处理:对照组(CON, n = 10/周期)和莫能菌素组(MON, n = 10/周期)。莫能菌素处理为每头奶牛34 g干酒糟(DDG)中添加300 mg莫能菌素,对照组只添加DDG,两组均在TMR上每天进行顶饲。奶牛挤奶3次/d,饲养在沙床独立式牛舍内,并设置卡兰门,收集个体DMI。使用GreenFeed系统测量肠道气体输出。研究期间测量了每头奶牛的日产奶量和DMI。此外,每周测量牛奶中脂肪、蛋白质和乳糖的百分比。处理间平均(±SEM) DMI (MON = 25.4±0.3,CON = 25.4±0.3,kg/d)和ECM (MON = 35.8±1.09,CON = 36.4±1.09,kg/d)相似。相反,莫能菌素组日CH4显著降低(MON = 207.1±13.1 g/d, CON = 257.2±13.1 g/d)。甲烷产率随处理显著下降(MON = 8.1±0.4 g/kg DMI, CON = 9.9±0.4 g/kg DMI)。最后,随着莫能菌素的增加(MON = 5.7±0.3 g/kg ECM, CON = 6.5±0.3 g/kg ECM),相对于ECM的甲烷强度呈下降趋势。结果表明,莫能菌素可减少奶牛每日肠道CH4排放和产量,为其用于减少奶牛CH4排放提供了证据。
{"title":"Monensin reduces enteric methane emissions in late-lactation Holstein cows fed high-concentrate diets","authors":"D. Onan-Martinez , M.A.T. de Bari , H. Olmo , J. Lance , I.M. Toledo , J.M. Tricarico , G.E. Dahl","doi":"10.3168/jdsc.2025-0865","DOIUrl":"10.3168/jdsc.2025-0865","url":null,"abstract":"<div><div>Methane is a major GHG produced by cattle. Strategies to reduce enteric methane include supplementation with monensin, which has shown variable outcomes. Therefore, we hypothesized that monensin (Rumensin, Elanco, Greenfield, IN) would reduce enteric CH<sub>4</sub> yield and intensity in lactating dairy cows (∼207 DIM). Twenty cows were enrolled in a 2 4-wk period crossover completely randomized design with repeated measures including 2 treatments: control (CON, n = 10/period) and monensin (MON, n = 10/period). Monensin treatment consisted of 300 mg of monensin in 34 g/cow of dried distillers grains (DDG) whereas CON was DDG alone, both top-dressed daily on the TMR. Cows were milked 3 times/d and housed in a sand-bedded freestall barn with Calan gates to collect individual DMI. A GreenFeed system was used to measure enteric gas output. Daily milk yield and DMI were measured from each cow during the study. Additionally, fat, protein and lactose percentages were measured weekly in milk. Average (±SEM) DMI (MON = 25.4 ± 0.3, CON = 25.4 ± 0.3, kg/d) and ECM (MON = 35.8 ± 1.09, CON = 36.4 ± 1.09; kg/d) were similar between treatments. Conversely, daily CH<sub>4</sub> was significantly reduced with monensin (MON = 207.1 ± 13.1, CON = 257.2 ± 13.1 g/d). Methane yield showed a significant decline with treatment (MON = 8.1 ± 0.4, CON = 9.9 ± 0.4 g/kg DMI). Last, methane intensity relative to ECM tended to decline with monensin (MON = 5.7 ± 0.3, CON = 6.5 ± 0.3 g/kg of ECM). The results suggest that monensin reduces daily enteric CH<sub>4</sub> emissions and yield in dairy cattle, providing evidence to support its use to reduce CH<sub>4</sub> emissions from cattle.</div></div>","PeriodicalId":94061,"journal":{"name":"JDS communications","volume":"7 1","pages":"Pages 34-39"},"PeriodicalIF":2.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145915387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bifidobacterium, a genus commonly found in the microbiome of healthy infants, has been demonstrated to exert various beneficial effects on the gastrointestinal tract, contributing to overall health. Notably, these bacteria possess the ability to use complex glycoconjugates as nutrients, which holds importance for breast-fed infants, as human milk contains substantial quantities of these glycoconjugates. However, the underlying mechanisms of action of these beneficial properties remain largely unexplored. This study presents an alternative perspective on the nutrients in the diet that could potentially support the maintenance of a healthy Bifidobacterium population. We present evidence suggesting that the presence of milk fat globule membrane (MFGM), a component shared by all mammalian milk, significantly alters the metabolism of these bacteria. This alteration is reflected in the production of exopolysaccharides on the surface and secretome of the bacteria fed MFGM, whereas the absence of this component does not result in such changes. To substantiate this hypothesis, we employed proteomic and physicochemical methods, culminating in the evidence of the MFGM layer through electron microscopy.
{"title":"Evidence for the influence of the milk fat globule membrane on bifidobacteria metabolism and cell surface properties","authors":"Celeste Miller, Rafael Jiménez-Flores","doi":"10.3168/jdsc.2025-0873","DOIUrl":"10.3168/jdsc.2025-0873","url":null,"abstract":"<div><div><em>Bifidobacterium</em>, a genus commonly found in the microbiome of healthy infants, has been demonstrated to exert various beneficial effects on the gastrointestinal tract, contributing to overall health. Notably, these bacteria possess the ability to use complex glycoconjugates as nutrients, which holds importance for breast-fed infants, as human milk contains substantial quantities of these glycoconjugates. However, the underlying mechanisms of action of these beneficial properties remain largely unexplored. This study presents an alternative perspective on the nutrients in the diet that could potentially support the maintenance of a healthy <em>Bifidobacterium</em> population. We present evidence suggesting that the presence of milk fat globule membrane (MFGM), a component shared by all mammalian milk, significantly alters the metabolism of these bacteria. This alteration is reflected in the production of exopolysaccharides on the surface and secretome of the bacteria fed MFGM, whereas the absence of this component does not result in such changes. To substantiate this hypothesis, we employed proteomic and physicochemical methods, culminating in the evidence of the MFGM layer through electron microscopy.</div></div>","PeriodicalId":94061,"journal":{"name":"JDS communications","volume":"7 1","pages":"Pages 12-17"},"PeriodicalIF":2.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145915541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}