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Impacts of feeding milk replacer supplemented with increasing concentrations of choline on feed intake, growth, and scouring incidence for 7 weeks preweaning and 1-week postweaning. 在断奶前 7 周和断奶后 1 周内,饲喂添加了浓度不断增加的胆碱的代乳粉对饲料摄入量、生长和冲刷发生率的影响。
Pub Date : 2024-05-01 DOI: 10.3168/jdsc.2024-0557
Anay D. Ravelo, Ellan I. Dufour, Makaila Klejeski, Bruce Ziegler, Angie Golombeski, I. Salfer
: The objective of this study was to evaluate the effect of increasing choline supplementation on the intake of milk replacer (MR) and calf starter (CS), growth performance, and fecal scores of calves over 8 weeks. One hundred calves from commercial herds were transported to the University of Minnesota Southern Research and Outreach Center (SROC) calf facility randomized to one of 4 treatments differing in choline supplementation within MR including: 0 ( C0 ; n = 24), 700 ( C700 ; n = 26), 1400 ( C1400 ; n = 25), and 2100 mg/kg choline ( C2100 ; n = 25). MR contained a basal concentration of 1650 mg/kg MR resulting in total choline concentrations in the MR of 1650 mg/kg for C0 was, 2350 mg/kg for C700 was, 3050 mg/kg for C1400 , and 3750 mg/kg for C2100 . Calves were weaned at 49 d, and the trial ended at 56 d. Body weight (BW) was measured at d 0, 14, 28, 42, 49 and 56. Fecal scores were measured weekly. Feed intake was measured daily and calculated bi-weekly. All feeding, body weight measurements, and fecal scoring were conducted by the research staff at the University of Minnesota SROC. Growth, feed intake, and gain to feed ratio were analyzed using a linear mixed effects model with choline concentration as a fixed effect and source herd and room as random effects. Initial body weight was included as a covariate for average daily gain. Fecal scores were analyzed as a chi-squared test of significance. Feeding 700 mg/kg supplemental choline increased MR intake throughout the preweaning period and increased ADG in both the preweaning and postweaning periods compared with the other treatments. No differences were observed for fecal scores across treatments. Results suggest that calf performance can be improved with MR containing 2350 mg/kg choline, contrasting with current NASEM (2021) requirements of 1000 mg/kg.
:本研究的目的是评估增加胆碱补充量对代乳粉(MR)和犊牛开食料(CS)的摄入量、生长性能以及犊牛 8 周内粪便评分的影响。100 头来自商业牛群的犊牛被运送到明尼苏达大学南方研究与推广中心(SROC)的犊牛饲养场,随机分配到 4 个不同的代乳品胆碱补充剂处理中的一个,包括0(C0;n = 24)、700(C700;n = 26)、1400(C1400;n = 25)和 2100 mg/kg 胆碱(C2100;n = 25)。MR 含有 1650 毫克/千克 MR 的基础浓度,因此 MR 中的总胆碱浓度为:C0 为 1650 毫克/千克,C700 为 2350 毫克/千克,C1400 为 3050 毫克/千克,C2100 为 3750 毫克/千克。犊牛在第 49 天断奶,试验在第 56 天结束。在第 0、14、28、42、49 和 56 天测量体重 (BW)。每周测量粪便评分。每天测量饲料摄入量,每两周计算一次。所有饲喂、体重测量和粪便评分均由明尼苏达大学 SROC 的研究人员进行。采用线性混合效应模型对生长、采食量和增重饲料比进行分析,胆碱浓度为固定效应,来源牛群和房间为随机效应。初始体重作为平均日增重的协变量。粪便评分采用卡方检验进行显著性分析。与其他处理相比,饲喂 700 mg/kg 补充胆碱可增加整个断奶前期的 MR 摄入量,并提高断奶前期和断奶后期的 ADG。各处理的粪便评分无差异。结果表明,使用含 2350 毫克/千克胆碱的 MR 可以提高犊牛的生产性能,而目前 NASEM(2021 年)的要求是 1000 毫克/千克。
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引用次数: 0
Confirmed bacteriological diagnosis and cure of non-severe Gram-positive clinical mastitis cases enrolled in a randomized clinical trial based on results of on-farm culture 根据农场培养结果对随机临床试验中的非严重革兰氏阳性临床乳腺炎病例进行确诊和治愈
Pub Date : 2024-05-01 DOI: 10.3168/jdsc.2024-0560
Q. K. Kolar, S.M. Godden, R.J. Erskine, P. L. Ruegg
: Our objectives were to describe the confirmed bacteriological diagnosis of mastitis pathogens cultured from cases of non-severe clinical mastitis (CM) identified as Gram-positive (GP) using on-farm culture and to compare differences in bacteriological cure (BC) based on antimicrobial (AM) therapy. Cows that developed non-severe CM in a single quarter were enrolled in a randomized clinical trial based on microbial growth on GP selective agars. Cows were randomly assigned to receive a once-daily intramammary treatment: 3d hetacillin (n = 69), 3d ceftiofur (n = 69), 8d ceftiofur (n = 70) or to a non-treated group (n = 32). Etiologies were confirmed with MALDI-TOF using frozen duplicate milk samples. The crude proportion of BC was determined using milk samples collected at 14, 21 and 28 d after enrollment for cases caused by Streptococci , non-aureus staphylococci (NAS), Strep-like organisms (consisting of Enterococcus and Lactococcus. ; SLO) and Staph. aureus. Multivariable models were used to determine the effect of AM treatment on BC cure at d 21 for a subset of cases that had complete data for all covariates (n = 88). Growth on GP agar resulted in enrollment of 240 cases with confirmed etiologies distributed as Strep. spp (21.7%; n = 52), Lact. spp (19.2%; n = 46), NAS (16.3%; n = 39), Staph aureus (6.3%; n = 16), Entero spp (5.0%; n = 12) and others (10%; n = 24). Thirty-five (14.5%) duplicate milk samples collected from enrolled cows had no significant growth in the lab while 16 (6.7%) were contaminated. Among sampling dates, combined crude BC for cases caused by target pathogens ranged from 68 to 71%. Crude BC ranged from 58 to 73% for the small group of non-treated cases (n = 12) and 69–71% for the combined group of cases that received IMM AM (n = 113). Among all groups and all follow-up dates, crude BC was least for cases caused by Staph. aureus (ranging from 33 to 43%), followed by cases caused by SLO (58–67%), NAS (79–80%) and Strep. spp. (81–91%). The proportion of BC at 21d (LSM ± SE) did not vary between all cases that received AM (0.77 ± 0.06) and cases in the non-treated group (0.73 ± 0.16). The odds of BC at 21d was about 5x greater for cases that occurred in primiparous as compared with multiparous cows and decreased 1.3-fold for each 1 log unit increase in SCC before the case. While this study does not have sufficient power to detect small differences in BC among pathogen groups or treatments, our results demonstrate that CM is caused by a wide diversity of GP bacteria with varying ranges of BC. These results provide justification for inclusion of negative control groups in future studies and confirm that important characteristics such as
:我们的目标是描述从非重症临床乳腺炎(CM)病例中培养出的细菌学确诊病原体,这些病原体通过农场培养被鉴定为革兰氏阳性(GP),并比较基于抗菌药(AM)疗法的细菌学治愈(BC)差异。根据微生物在 GP 选择性琼脂上的生长情况,将在一个季度内出现非严重 CM 的奶牛纳入随机临床试验。奶牛被随机分配接受每天一次的乳房内治疗:3d hetacillin(n = 69)、3d ceftiofur(n = 69)、8d ceftiofur(n = 70)或未接受治疗组(n = 32)。使用冷冻的重复牛奶样本通过 MALDI-TOF 确认病因。利用入组后 14、21 和 28 天采集的牛奶样本,确定由链球菌、非金黄色葡萄球菌 (NAS)、类链球菌(由肠球菌和乳球菌组成;SLO)和金黄色葡萄球菌引起的 BC 病例的粗比例。对于所有协变量数据完整的病例子集(n = 88),采用多变量模型确定 AM 治疗对 BC 第 21 天治愈的影响。在 GP 琼脂上生长的 240 个病例确认了病因,病因分布为链球菌(21.7%;n = 52)、乳杆菌(19.2%;n = 46)、NAS(16.3%;n = 39)、金黄色葡萄球菌(6.3%;n = 16)、肠杆菌(5.0%;n = 12)和其他(10%;n = 24)。从登记奶牛身上采集的 35 份(14.5%)重复牛奶样本在实验室中没有明显生长,而 16 份(6.7%)受到污染。在不同的采样日期,由目标病原体引起的病例的综合粗 BC 为 68% 至 71%。未接受治疗的一小群病例(12 例)的粗 BC 为 58% 至 73%,接受 IMM AM 治疗的病例(113 例)的粗 BC 为 69% 至 71%。在所有组别和所有随访日期中,由金黄色葡萄球菌引起的病例的粗 BC 最低(33% 至 43%),其次是由 SLO(58% 至 67%)、NAS(79% 至 80%)和链球菌(81% 至 91%)引起的病例。所有接受AM治疗的病例(0.77 ± 0.06)和未接受AM治疗组的病例(0.73 ± 0.16)在21天时出现BC的比例(LSM ± SE)没有差异。与多胎奶牛相比,初产奶牛在21天时发生BC的几率约为多胎奶牛的5倍,病例发生前SCC每增加1个对数单位,BC几率下降1.3倍。虽然本研究没有足够的能力检测病原体组或治疗方法之间 BC 的微小差异,但我们的结果表明,CM 是由多种 GP 细菌引起的,其 BC 范围各不相同。这些结果为在今后的研究中纳入阴性对照组提供了理由,并证实了一些重要特征,如
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引用次数: 0
Variation in partial direct costs of dry cow therapy on 37 large dairy herds 37 个大型奶牛场干奶牛治疗部分直接成本的变化
Pub Date : 2024-05-01 DOI: 10.3168/jdsc.2024-0568
Pamela L. Ruegg, J. L. D. Campos, A. Kates, A. Steinberger, A. Sethi, G. Suen, John Shutske, N. Safdar, Tony Goldberg, P. L. Ruegg8
: The objective of this observational study was to estimate partial direct costs of dry cow antibiotic therapy (DCT) protocols used on 37 large dairy herds in Wisconsin and to estimate the potential monetary savings and reduction in antimicrobial usage (AMU) if selective DCT was used. Partial direct costs of DCT were calculated using costs of intramammary (IMM) antimicrobials and teat seal-ants. Data were retrospectively collected on 37 large dairy farms for a period of one year and included the total number of cows dried-off, types of IMM antimicrobial used, and product
:这项观察性研究的目的是估算威斯康星州 37 个大型奶牛场使用干奶牛抗生素疗法(DCT)方案的部分直接成本,并估算如果使用选择性 DCT,可能节省的资金和减少的抗菌药用量(AMU)。使用乳房内 (IMM) 抗菌剂和乳头密封剂的成本计算了 DCT 的部分直接成本。对 37 个大型奶牛场进行了为期一年的回顾性数据收集,数据包括奶牛干化的总头数、使用的 IMM 抗菌剂类型和产品名称。
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引用次数: 0
Characterization of an in vitro model to study CD4+ T cells metabolism in dairy cows 研究奶牛 CD4+ T 细胞代谢的体外模型的特征
Pub Date : 2024-05-01 DOI: 10.3168/jdsc.2024-0565
U. Arshad, M. Cid de la Paz, H.M. White, L.R. Cangiano
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引用次数: 0
Effects of lactose content in milk replacer on apparent digestibility, growth, liver mRNA expression, and blood parameters related to metabolism of dairy calves 代乳粉中乳糖含量对奶牛表观消化率、生长、肝脏 mRNA 表达以及与新陈代谢有关的血液参数的影响
Pub Date : 2024-05-01 DOI: 10.3168/jdsc.2023-0528
R. Fukumori, M. Hirose, I. Norimura, T. Nakayama, K. Shimada, H. Mineo, M. Steele, S. Gondaira, H. Higuchi, K. Chisato, S. Oikawa, K. Izumi
: Even with the same energy supply, differences in energy sources may affect calf growth and metabolism. In this study, we evaluated the effects of feeding 3 different milk replacers (MRs) with different lactose supplies under isoenergetic conditions on calf digestibility, growth, and metabolism-related markers. Fifteen Holstein bull calves were randomly assigned to one of 3 MR-feeding treatments: low-lactose (L:38%), medium-lactose (M:41%), or high-lactose (H:46%). After birth, calves were provided colostrum and treatment MRs were fed from 1 d of age and gradually increased to a maximum feeding rate at 20 d of age (L:1.16 kg/d, M:1.21 kg/d, H:1.26 kg/d DM) without feeding solid feeds during the experimental period. Blood samples were temporarily collected weekly to assess blood concentrations of metabolites and hormones. From 30 to 36 d of age, the calves were euthanized and liver samples were collected to determine growth-related mRNA expression. L calves showed a greater body length than H calves and the highest GHR mRNA expression. Plasma concentrations of total cholesterol, urea nitrogen, total protein, albumin, insulin, and insulin-growth factor-1 were not different, but plasma concentrations of triglycerides were higher in order H, M, and L. These results showed that the difference in lactose content in the MR affected calf metabolism, and the L-MR was suggested to be more likely to enhance growth into the peripheral tissues.
:即使能量供应相同,能量来源的不同也会影响犊牛的生长和新陈代谢。在这项研究中,我们评估了在等能量条件下饲喂 3 种不同乳糖供应量的代乳品(MR)对犊牛消化率、生长和代谢相关指标的影响。15 头荷斯坦公牛犊牛被随机分配到 3 种 MR 饲喂处理中的一种:低乳糖(L:38%)、中乳糖(M:41%)或高乳糖(H:46%)。犊牛出生后,为其提供初乳,并从 1 日龄开始饲喂 MR,在 20 日龄时逐渐增加到最大饲喂量(L:1.16 kg/d,M:1.21 kg/d,H:1.26 kg/d DM),实验期间不饲喂固体饲料。每周临时采集血液样本,以评估血液中代谢物和激素的浓度。在犊牛出生后 30 至 36 d,对其实施安乐死,并采集肝脏样本以测定与生长相关的 mRNA 表达。L型小牛的体长大于H型小牛,GHR mRNA表达量最高。血浆中总胆固醇、尿素氮、总蛋白、白蛋白、胰岛素和胰岛素生长因子-1的浓度没有差异,但血浆中甘油三酯的浓度依次高于H、M和L。
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引用次数: 0
Hemoconcentration differs in neonatal dairy calves: considerations for assessment of transfer of passive immunity 新生乳牛的血液浓度不同:评估被动免疫转移的考虑因素
Pub Date : 2024-05-01 DOI: 10.3168/jdsc.2024-0570
Sabine Mann, T. Westhoff, R. A. Molano, M. E. V. Amburgh, S. Mann1
: Assessing transfer of passive immunity (TPI) is a critical management strategy to evaluate colostrum management and feeding; however, variability in hemoconcentration or serum or plasma volume in calves might influence TPI assessment. The objectives of this study were to 1) describe the variability in hemoconcentration as well as TPI in Holstein calves in New York State and 2) describe the effect of adjusting total protein (TP) for the degree of hemoconcentration by applying a sample average proportion of plasma in blood (PP) on TPI assessment. Records of TP and PP from 703 1 to 9 d of age Holstein calves from 19 commercial dairy farms were analyzed. The PP was determined by centrifugation of microhematocrit tubes and serum, and plasma TP was determined by digital refractometry. Transfer of passive immunity was categorized using unadjusted TP (uTP) as excellent = ≥ 6
:评估被动免疫转移(TPI)是评估初乳管理和饲喂的一项重要管理策略;然而,犊牛血液浓缩或血清或血浆容量的变化可能会影响 TPI 评估。本研究的目的是:1)描述纽约州荷斯坦犊牛血液浓缩和 TPI 的变异性;2)通过应用血浆在血液中的样本平均比例 (PP),描述根据血液浓缩程度调整总蛋白 (TP) 对 TPI 评估的影响。对来自 19 个商业奶牛场的 703 头 1 到 9 日龄荷斯坦犊牛的 TP 和 PP 记录进行了分析。PP是通过离心微血细胞比容管和血清测定的,血浆TP是通过数字折射仪测定的。用未经调整的 TP(uTP)对被动免疫的转移进行分类,即优良 = ≥ 6
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引用次数: 0
A note from the Editor in Chief of JDS Communications: Connecting via coffee and ice cream JDS Communications 总编辑的说明:通过咖啡和冰淇淋建立联系
Pub Date : 2024-04-10 DOI: 10.3168/jdsc.2024-0578
JessicaA.A. McArt Editor in Chief
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引用次数: 0
Specificity of two peripartum blood markers for early lactation acute uterine inflammation in pasture-fed, seasonal-calving dairy cows. 两种围产期血液标记物对牧草喂养、季节性产犊奶牛泌乳早期急性子宫炎症的特异性。
Pub Date : 2024-04-01 DOI: 10.3168/jdsc.2023-0509
O. Spaans, J. R. Roche, C. Burke, C. Phyn, S. McDougall, C. Reed, B. Kuhn-Sherlock, A. Hickey, A. Heiser, M. Crookenden
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引用次数: 0
ST8-t008-SCC IV methicillin-resistant Staphyloccoccus aureus (MRSA) in retail fresh cheese 零售新鲜奶酪中的 ST8-t008-SCC IV 型耐甲氧西林金黄色葡萄球菌(MRSA)
Pub Date : 2024-04-01 DOI: 10.3168/jdsc.2023-0521
Carolina Chaves, Melissa Montenegro, Hyrina Piedra, Marta Pérez-Villanueva, César Rodríguez
{"title":"ST8-t008-SCC IV methicillin-resistant Staphyloccoccus aureus (MRSA) in retail fresh cheese","authors":"Carolina Chaves, Melissa Montenegro, Hyrina Piedra, Marta Pérez-Villanueva, César Rodríguez","doi":"10.3168/jdsc.2023-0521","DOIUrl":"https://doi.org/10.3168/jdsc.2023-0521","url":null,"abstract":"","PeriodicalId":94061,"journal":{"name":"JDS communications","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140762646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluation of serum concentrations of total protein and gamma-globulin as an indicator of serum immunoglobulin G concentration in dairy calves 将血清总蛋白和伽马球蛋白浓度作为奶牛血清免疫球蛋白 G 浓度的指标进行评估
Pub Date : 2024-04-01 DOI: 10.3168/jdsc.2023-0469
K. Murayama, N. Kobayashi, N. Nishizawa, M. Oba, T. Sugino
{"title":"Evaluation of serum concentrations of total protein and gamma-globulin as an indicator of serum immunoglobulin G concentration in dairy calves","authors":"K. Murayama, N. Kobayashi, N. Nishizawa, M. Oba, T. Sugino","doi":"10.3168/jdsc.2023-0469","DOIUrl":"https://doi.org/10.3168/jdsc.2023-0469","url":null,"abstract":"","PeriodicalId":94061,"journal":{"name":"JDS communications","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140793112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
JDS communications
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