Previous studies on the kinematics of pea plants' ascent and attach behavior have demonstrated that the signature of their movement varies depending on the kind of support. So far, these studies have been confined to artificial supports (e.g. wooden sticks). Little is known regarding the conditions under which pea plants could rely on biological supports (e.g. neighboring plants) for climbing toward the light. In this study, we capitalize on the 3D kinematic analysis of movement to ascertain whether pea plants scale their kinematics differently depending on whether they aim for artificial or biological support. Results suggest that biological support determines a smoother and more accurate behavior than that elicited by the artificial one. These results shed light on pea plants' ability to detect and classify the properties of objects and implement a movement plan attuned to the very nature of the support. We contend that such differences depend on the augmented multisensory experience elicited by the biological support.
{"title":"Artificial and biological supports are different for pea plants.","authors":"Bianca Bonato, Valentina Simonetti, Silvia Guerra, Umberto Castiello","doi":"10.1080/15592324.2024.2355739","DOIUrl":"10.1080/15592324.2024.2355739","url":null,"abstract":"<p><p>Previous studies on the kinematics of pea plants' ascent and attach behavior have demonstrated that the signature of their movement varies depending on the kind of support. So far, these studies have been confined to artificial supports (e.g. wooden sticks). Little is known regarding the conditions under which pea plants could rely on biological supports (e.g. neighboring plants) for climbing toward the light. In this study, we capitalize on the 3D kinematic analysis of movement to ascertain whether pea plants scale their kinematics differently depending on whether they aim for artificial or biological support. Results suggest that biological support determines a smoother and more accurate behavior than that elicited by the artificial one. These results shed light on pea plants' ability to detect and classify the properties of objects and implement a movement plan attuned to the very nature of the support. We contend that such differences depend on the augmented multisensory experience elicited by the biological support.</p>","PeriodicalId":94172,"journal":{"name":"Plant signaling & behavior","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7616103/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141249271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
G protein-coupled receptors (GPCRs) constitute the largest family of transmembrane proteins in metazoans that mediate the regulation of various physiological responses to discrete ligands through heterotrimeric G protein subunits. The existence of GPCRs in plant is contentious, but their comparable crucial role in various signaling pathways necessitates the identification of novel remote GPCR-like proteins that essentially interact with the plant G protein α subunit and facilitate the transduction of various stimuli. In this study, we identified three putative GPCR-like proteins (OsGPCRLPs) (LOC_Os06g09930.1, LOC_Os04g36630.1, and LOC_Os01g54784.1) in the rice proteome using a stringent bioinformatics workflow. The identified OsGPCRLPs exhibited a canonical GPCR 'type I' 7TM topology, patterns, and biologically significant sites for membrane anchorage and desensitization. Cluster-based interactome mapping revealed that the identified proteins interact with the G protein α subunit which is a characteristic feature of GPCRs. Computational results showing the interaction of identified GPCR-like proteins with G protein α subunit and its further validation by the membrane yeast-two-hybrid assay strongly suggest the presence of GPCR-like 7TM proteins in the rice proteome. The absence of a regulator of G protein signaling (RGS) box in the C- terminal domain, and the presence of signature motifs of canonical GPCR in the identified OsGPCRLPs strongly suggest that the rice proteome contains GPCR-like proteins that might be involved in signal transduction.
G 蛋白偶联受体(GPCR)是元类动物中最大的跨膜蛋白家族,通过异三聚体 G 蛋白亚基介导对离散配体的各种生理反应的调节。植物中是否存在 GPCR 尚存争议,但由于它们在各种信号通路中发挥着相当关键的作用,因此有必要鉴定新型的远端 GPCR 类蛋白,这些蛋白本质上与植物 G 蛋白 α 亚基相互作用,促进各种刺激的转导。在本研究中,我们采用严格的生物信息学工作流程,在水稻蛋白质组中鉴定出了三个假定的类 GPCR 蛋白(OsGPCRLPs)(LOC_Os06g09930.1、LOC_Os04g36630.1 和 LOC_Os01g54784.1)。鉴定出的 OsGPCRLPs 具有典型的 GPCR "I 型 "7TM 拓扑结构、模式以及具有生物学意义的膜锚定和脱敏位点。基于聚类的相互作用组图谱显示,鉴定出的蛋白质与 G 蛋白 α 亚基相互作用,这是 GPCR 的一个特征。计算结果显示了已鉴定的类 GPCR 蛋白与 G 蛋白 α 亚基的相互作用,并通过膜酵母-双杂交试验进一步验证了这一点,这有力地表明了水稻蛋白质组中存在类 GPCR 7TM 蛋白。在已鉴定的 OsGPCRLPs 的 C 端结构域中没有 G 蛋白信号转导调节器(RGS)框,而且存在典型 GPCR 的标志性基序,这些都有力地表明水稻蛋白质组中含有可能参与信号转导的 GPCR 样蛋白。
{"title":"Proteome-wide analysis reveals G protein-coupled receptor-like proteins in rice (<i>Oryza sativa</i>).","authors":"Dinesh K Yadav, Gyan Prakash Srivastava, Ananya Singh, Madhavi Singh, Neelam Yadav, Narendra Tuteja","doi":"10.1080/15592324.2024.2365572","DOIUrl":"10.1080/15592324.2024.2365572","url":null,"abstract":"<p><p>G protein-coupled receptors (GPCRs) constitute the largest family of transmembrane proteins in metazoans that mediate the regulation of various physiological responses to discrete ligands through heterotrimeric G protein subunits. The existence of GPCRs in plant is contentious, but their comparable crucial role in various signaling pathways necessitates the identification of novel remote GPCR-like proteins that essentially interact with the plant G protein α subunit and facilitate the transduction of various stimuli. In this study, we identified three putative GPCR-like proteins (OsGPCRLPs) (LOC_Os06g09930.1, LOC_Os04g36630.1, and LOC_Os01g54784.1) in the rice proteome using a stringent bioinformatics workflow. The identified OsGPCRLPs exhibited a canonical GPCR 'type I' 7TM topology, patterns, and biologically significant sites for membrane anchorage and desensitization. Cluster-based interactome mapping revealed that the identified proteins interact with the G protein α subunit which is a characteristic feature of GPCRs. Computational results showing the interaction of identified GPCR-like proteins with G protein α subunit and its further validation by the membrane yeast-two-hybrid assay strongly suggest the presence of GPCR-like 7TM proteins in the rice proteome. The absence of a regulator of G protein signaling (RGS) box in the C- terminal domain, and the presence of signature motifs of canonical GPCR in the identified OsGPCRLPs strongly suggest that the rice proteome contains GPCR-like proteins that might be involved in signal transduction.</p>","PeriodicalId":94172,"journal":{"name":"Plant signaling & behavior","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11195488/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141433737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-31Epub Date: 2024-01-08DOI: 10.1080/15592324.2023.2298053
Khushboo Khator, Suman Parihar, Jan Jasik, Gyan Singh Shekhawat
Plants, as sessile organisms, are subjected to diverse abiotic stresses, including salinity, desiccation, metal toxicity, thermal fluctuations, and hypoxia at different phases of plant growth. Plants can activate messenger molecules to initiate a signaling cascade of response toward environmental stresses that results in either cell death or plant acclimation. Nitric oxide (NO) is a small gaseous redox-active molecule that exhibits a plethora of physiological functions in growth, development, flowering, senescence, stomata closure and responses to environmental stresses. It can also facilitate alteration in protein function and reprogram the gene profiling by direct or indirect interaction with different target molecules. The bioactivity of NO can be manifested through different redox-based protein modifications including S-nitrosylation, protein nitration, and metal nitrosylation in plants. Although there has been considerable progress in the role of NO in regulating stress signaling, still the physiological mechanisms regarding the abiotic stress tolerance in plants remain unclear. This review summarizes recent advances in understanding the emerging knowledge regarding NO function in plant tolerance against abiotic stresses. The manuscript also highlighted the importance of NO as an abiotic stress modulator and developed a rational design for crop cultivation under a stress environment.
植物作为无梗生物,在其生长的不同阶段会受到各种非生物胁迫,包括盐度、干燥、金属毒性、热波动和缺氧。植物可以激活信使分子,启动信号级联反应,以应对环境胁迫,从而导致细胞死亡或植物适应环境。一氧化氮(NO)是一种具有氧化还原作用的气态小分子,在生长、发育、开花、衰老、气孔关闭和对环境胁迫的反应中具有多种生理功能。它还能通过与不同靶分子的直接或间接相互作用,促进蛋白质功能的改变和基因谱的重编程。氮氧化物的生物活性可通过不同的氧化还原型蛋白质修饰来体现,包括植物体内的 S-亚硝基化、蛋白质硝化和金属亚硝基化。尽管在氮氧化物调节胁迫信号转导的作用方面取得了很大进展,但植物耐受非生物胁迫的生理机制仍不清楚。这篇综述总结了在了解有关 NO 在植物耐受非生物胁迫中的功能的新知识方面的最新进展。稿件还强调了 NO 作为一种非生物胁迫调节剂的重要性,并对胁迫环境下的作物栽培进行了合理设计。
{"title":"Nitric oxide in plants: an insight on redox activity and responses toward abiotic stress signaling.","authors":"Khushboo Khator, Suman Parihar, Jan Jasik, Gyan Singh Shekhawat","doi":"10.1080/15592324.2023.2298053","DOIUrl":"10.1080/15592324.2023.2298053","url":null,"abstract":"<p><p>Plants, as sessile organisms, are subjected to diverse abiotic stresses, including salinity, desiccation, metal toxicity, thermal fluctuations, and hypoxia at different phases of plant growth. Plants can activate messenger molecules to initiate a signaling cascade of response toward environmental stresses that results in either cell death or plant acclimation. Nitric oxide (NO) is a small gaseous redox-active molecule that exhibits a plethora of physiological functions in growth, development, flowering, senescence, stomata closure and responses to environmental stresses. It can also facilitate alteration in protein function and reprogram the gene profiling by direct or indirect interaction with different target molecules. The bioactivity of NO can be manifested through different redox-based protein modifications including <i>S</i>-nitrosylation, protein nitration, and metal nitrosylation in plants. Although there has been considerable progress in the role of NO in regulating stress signaling, still the physiological mechanisms regarding the abiotic stress tolerance in plants remain unclear. This review summarizes recent advances in understanding the emerging knowledge regarding NO function in plant tolerance against abiotic stresses. The manuscript also highlighted the importance of NO as an abiotic stress modulator and developed a rational design for crop cultivation under a stress environment.</p>","PeriodicalId":94172,"journal":{"name":"Plant signaling & behavior","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10793691/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139405809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-31Epub Date: 2024-03-21DOI: 10.1080/15592324.2024.2332017
Khaled Moustafa
{"title":"Maize's origin to be revisited.","authors":"Khaled Moustafa","doi":"10.1080/15592324.2024.2332017","DOIUrl":"10.1080/15592324.2024.2332017","url":null,"abstract":"","PeriodicalId":94172,"journal":{"name":"Plant signaling & behavior","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10962592/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140186761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-31Epub Date: 2024-05-30DOI: 10.1080/15592324.2024.2360298
Kilian Lucas Ossetek, Andrea Teresa Müller, Axel Mithöfer
This study investigated the accumulation of phenlyacetaldoxime (PAOx) and PAOx-Glc in Tococa quadrialata leaves in response to herbivore infestation and mechanical wounding. Results show that PAOx levels peaked at 24 h post-infestation, while PAOx-Glc remained present for several days. The accumulation of PAOx began as early as 3 h after herbivory, with PAOx-Glc significantly increased after 6 h. Mechanical wounding induced similar responses in PAOx and PAOx-Glc accumulation as herbivory, suggesting that continuous tissue damage triggers the production of these compounds. Interestingly, SpitWorm-treated leaves showed the highest levels of both PAOx and PAOx-Glc, indicating that herbivore-derived oral secretions (OS) play a role in the induction of these compounds. Additionally, JA-independent PAOx production was found to be associated with tissue damage rather than specific known signaling compounds. Emission of benzyl cyanide and 2-phenylethanol, PAOx-derived plant volatiles, was observed in response to herbivory and SpitWorm treatment providing plant-derived OS, further highlighting the role of herbivore cues in plant defense responses.
{"title":"Robotic mechanical wounding is sufficient to induce phenylacetaldoxime accumulation in <i>Tococa quadrialata</i>.","authors":"Kilian Lucas Ossetek, Andrea Teresa Müller, Axel Mithöfer","doi":"10.1080/15592324.2024.2360298","DOIUrl":"10.1080/15592324.2024.2360298","url":null,"abstract":"<p><p>This study investigated the accumulation of phenlyacetaldoxime (PAOx) and PAOx-Glc in <i>Tococa quadrialata</i> leaves in response to herbivore infestation and mechanical wounding. Results show that PAOx levels peaked at 24 h post-infestation, while PAOx-Glc remained present for several days. The accumulation of PAOx began as early as 3 h after herbivory, with PAOx-Glc significantly increased after 6 h. Mechanical wounding induced similar responses in PAOx and PAOx-Glc accumulation as herbivory, suggesting that continuous tissue damage triggers the production of these compounds. Interestingly, SpitWorm-treated leaves showed the highest levels of both PAOx and PAOx-Glc, indicating that herbivore-derived oral secretions (OS) play a role in the induction of these compounds. Additionally, JA-independent PAOx production was found to be associated with tissue damage rather than specific known signaling compounds. Emission of benzyl cyanide and 2-phenylethanol, PAOx-derived plant volatiles, was observed in response to herbivory and SpitWorm treatment providing plant-derived OS, further highlighting the role of herbivore cues in plant defense responses.</p>","PeriodicalId":94172,"journal":{"name":"Plant signaling & behavior","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11141477/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141176980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-31Epub Date: 2024-04-30DOI: 10.1080/15592324.2024.2345985
André Kessler, Michael B Mueller
Plant induced responses to environmental stressors are increasingly studied in a behavioral ecology context. This is particularly true for plant induced responses to herbivory that mediate direct and indirect defenses, and tolerance. These seemingly adaptive alterations of plant defense phenotypes in the context of other environmental conditions have led to the discussion of such responses as intelligent behavior. Here we consider the concept of plant intelligence and some of its predictions for chemical information transfer in plant interaction with other organisms. Within this framework, the flow, perception, integration, and storage of environmental information are considered tunable dials that allow plants to respond adaptively to attacking herbivores while integrating past experiences and environmental cues that are predictive of future conditions. The predictive value of environmental information and the costs of acting on false information are important drivers of the evolution of plant responses to herbivory. We identify integrative priming of defense responses as a mechanism that allows plants to mitigate potential costs associated with acting on false information. The priming mechanisms provide short- and long-term memory that facilitates the integration of environmental cues without imposing significant costs. Finally, we discuss the ecological and evolutionary prediction of the plant intelligence hypothesis.
{"title":"Induced resistance to herbivory and the intelligent plant.","authors":"André Kessler, Michael B Mueller","doi":"10.1080/15592324.2024.2345985","DOIUrl":"10.1080/15592324.2024.2345985","url":null,"abstract":"<p><p>Plant induced responses to environmental stressors are increasingly studied in a behavioral ecology context. This is particularly true for plant induced responses to herbivory that mediate direct and indirect defenses, and tolerance. These seemingly adaptive alterations of plant defense phenotypes in the context of other environmental conditions have led to the discussion of such responses as intelligent behavior. Here we consider the concept of plant intelligence and some of its predictions for chemical information transfer in plant interaction with other organisms. Within this framework, the flow, perception, integration, and storage of environmental information are considered tunable dials that allow plants to respond adaptively to attacking herbivores while integrating past experiences and environmental cues that are predictive of future conditions. The predictive value of environmental information and the costs of acting on false information are important drivers of the evolution of plant responses to herbivory. We identify integrative priming of defense responses as a mechanism that allows plants to mitigate potential costs associated with acting on false information. The priming mechanisms provide short- and long-term memory that facilitates the integration of environmental cues without imposing significant costs. Finally, we discuss the ecological and evolutionary prediction of the plant intelligence hypothesis.</p>","PeriodicalId":94172,"journal":{"name":"Plant signaling & behavior","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11062368/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140854549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-31Epub Date: 2024-06-24DOI: 10.1080/15592324.2024.2370719
Deepti Routray, Arindam Ghatak, Palak Chaturvedi, Linda Petijová, Wolfram Weckwerth, Dajana Ručová, Martin Bačkor, Ingeborg Lang, Michal Goga
Cyanobacterium Nostoc commune is a filamentous terrestrial prokaryotic organism widely distributed, which suggest its high adaptive potential to environmental or abiotic stress. Physiological parameters and proteomic analysis were performed in two accession of N. commune with the aim to elucidate the differences of physiological trails between distant geotypes, namely Antarctic (AN) and central European (CE). The result obtained clearly showed that the AN geotype demonstrates elevated levels of total phenols, flavonoids, carotenoids, and phycobiliproteins, indicative of its adaptation to environmental stress as referred by comparison to CE sample. Additionally, we employed LC-MS analysis to investigate the proteomes of N. commune from AN and CE geotypes. In total, 1147 proteins were identified, among which 646 proteins expressed significant (up-regulation) changes in both accessions. In the AN geotype, 83 exclusive proteins were identified compared to 25 in the CE geotype. Functional classification of the significant proteins showed a large fraction involved in photosynthesis, amino acid metabolism, carbohydrate metabolism and protein biosynthesis. Further analysis revealed some defense-related proteins such as, superoxide dismutase (SOD) and glutathione reductase, which are rather explicitly expressed in the AN N. commune. The last two proteins suggest a more stressful condition in AN N. commune. In summary, our findings highlight biochemical processes that safeguard the AN geotype of N. commune from extreme environmental challenges, not recorded in CE accession, probably due to less stressful environment in Europe. This study brings the first ever proteomic analysis of N. commune, emphasizing the need for additional investigations into the climate adaptation of this species with rather plastic genome.
蓝藻 Nostoc commune 是一种广泛分布的丝状陆生原核生物,这表明它对环境或非生物压力具有很高的适应潜力。研究人员对两株共生草履虫进行了生理参数和蛋白质组分析,旨在阐明南极(AN)和中欧(CE)这两个不同地理型之间生理径迹的差异。研究结果清楚地表明,与中欧样本相比,南极(AN)地理模式的总酚、类黄酮、类胡萝卜素和藻蓝蛋白水平较高,表明其对环境压力的适应性较强。此外,我们还采用 LC-MS 分析方法研究了 AN 和 CE 地理样型中的蒲公英蛋白质组。共鉴定出 1147 个蛋白质,其中 646 个蛋白质在两个品种中均有显著变化(上调)。在 AN 地理样型中,共鉴定出 83 个独有蛋白质,而在 CE 地理样型中只有 25 个。对重要蛋白质的功能分类显示,大部分蛋白质参与光合作用、氨基酸代谢、碳水化合物代谢和蛋白质的生物合成。进一步的分析表明,一些与防御有关的蛋白质,如超氧化物歧化酶(SOD)和谷胱甘肽还原酶,在 AN N. commune 中的表达相当明确。后两种蛋白质表明芒柄苣苔的应激条件更强。总之,我们的研究结果突显了保护赤桉树 AN 地理单元免受极端环境挑战的生化过程,这可能是由于欧洲的环境压力较小,CE 单元中并没有记录。这项研究首次对胭脂虫进行了蛋白质组学分析,强调了对这种基因组具有相当可塑性的物种的气候适应性进行更多研究的必要性。
{"title":"Comparative analysis of geotypic variations in the proteome of <i>Nostoc commune</i>.","authors":"Deepti Routray, Arindam Ghatak, Palak Chaturvedi, Linda Petijová, Wolfram Weckwerth, Dajana Ručová, Martin Bačkor, Ingeborg Lang, Michal Goga","doi":"10.1080/15592324.2024.2370719","DOIUrl":"10.1080/15592324.2024.2370719","url":null,"abstract":"<p><p>Cyanobacterium <i>Nostoc commune</i> is a filamentous terrestrial prokaryotic organism widely distributed, which suggest its high adaptive potential to environmental or abiotic stress. Physiological parameters and proteomic analysis were performed in two accession of <i>N. commune</i> with the aim to elucidate the differences of physiological trails between distant geotypes, namely Antarctic (AN) and central European (CE). The result obtained clearly showed that the AN geotype demonstrates elevated levels of total phenols, flavonoids, carotenoids, and phycobiliproteins, indicative of its adaptation to environmental stress as referred by comparison to CE sample. Additionally, we employed LC-MS analysis to investigate the proteomes of <i>N. commune</i> from AN and CE geotypes. In total, 1147 proteins were identified, among which 646 proteins expressed significant (up-regulation) changes in both accessions. In the AN geotype, 83 exclusive proteins were identified compared to 25 in the CE geotype. Functional classification of the significant proteins showed a large fraction involved in photosynthesis, amino acid metabolism, carbohydrate metabolism and protein biosynthesis. Further analysis revealed some defense-related proteins such as, superoxide dismutase (SOD) and glutathione reductase, which are rather explicitly expressed in the AN <i>N. commune</i>. The last two proteins suggest a more stressful condition in AN <i>N. commune</i>. In summary, our findings highlight biochemical processes that safeguard the AN geotype of <i>N. commune</i> from extreme environmental challenges, not recorded in CE accession, probably due to less stressful environment in Europe. This study brings the first ever proteomic analysis of <i>N. commune</i>, emphasizing the need for additional investigations into the climate adaptation of this species with rather plastic genome.</p>","PeriodicalId":94172,"journal":{"name":"Plant signaling & behavior","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11197914/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141447902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-31Epub Date: 2024-03-17DOI: 10.1080/15592324.2024.2329842
Kaleb Fransgo, Lei-Chen Lin, Hyungmin Rho
Blueberries confront substantial challenges from climate change, such as rising temperatures and extreme heat, necessitating urgent solutions to ensure productivity. We hypothesized that ericoid mycorrhizal fungi (ErM) and plant growth-promoting bacteria (PGPB) would establish symbiotic relationships and increase heat stress tolerance in blueberries. A growth chamber study was designed with low (25/20°C) and high temperature (35/30°C) conditions with micropropagated blueberry plantlets inoculated with ErM, PGPB, and both. Gas exchange and chlorophyll fluorescence properties of the leaves were monitored throughout the growth. At harvest, biochemical assays and biomass analysis were performed to evaluate potential oxidative stress induced by elevated temperatures. ErM application boosted root biomass under 25/20°C conditions but did not impact photosynthetic efficiency. In contrast, PGPB demonstrated a dual role: enhancing photosynthetic capacity and reducing stomatal conductance notably under 35/30°C conditions. Moreover, PGPB showcased conflicting effects, reducing oxidative damage under 25/20°C conditions while intensifying it during 47°C heat shock. A significant highlight lies in the opposing effects of ErM and PGPB on root growth and stomatal conductance, signifying their reciprocal influence on blueberry plant behavior, which may lead to increased water uptake or reduced water use. Understanding these complex interactions holds promise for refining sustainable strategies to overcome climate challenges.
{"title":"Distinct interactions of ericoid mycorrhizae and plant growth-promoting bacteria: impacts on blueberry growth and heat resilience.","authors":"Kaleb Fransgo, Lei-Chen Lin, Hyungmin Rho","doi":"10.1080/15592324.2024.2329842","DOIUrl":"10.1080/15592324.2024.2329842","url":null,"abstract":"<p><p>Blueberries confront substantial challenges from climate change, such as rising temperatures and extreme heat, necessitating urgent solutions to ensure productivity. We hypothesized that ericoid mycorrhizal fungi (ErM) and plant growth-promoting bacteria (PGPB) would establish symbiotic relationships and increase heat stress tolerance in blueberries. A growth chamber study was designed with low (25/20°C) and high temperature (35/30°C) conditions with micropropagated blueberry plantlets inoculated with ErM, PGPB, and both. Gas exchange and chlorophyll fluorescence properties of the leaves were monitored throughout the growth. At harvest, biochemical assays and biomass analysis were performed to evaluate potential oxidative stress induced by elevated temperatures. ErM application boosted root biomass under 25/20°C conditions but did not impact photosynthetic efficiency. In contrast, PGPB demonstrated a dual role: enhancing photosynthetic capacity and reducing stomatal conductance notably under 35/30°C conditions. Moreover, PGPB showcased conflicting effects, reducing oxidative damage under 25/20°C conditions while intensifying it during 47°C heat shock. A significant highlight lies in the opposing effects of ErM and PGPB on root growth and stomatal conductance, signifying their reciprocal influence on blueberry plant behavior, which may lead to increased water uptake or reduced water use. Understanding these complex interactions holds promise for refining sustainable strategies to overcome climate challenges.</p>","PeriodicalId":94172,"journal":{"name":"Plant signaling & behavior","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10950280/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140144906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-31Epub Date: 2024-03-25DOI: 10.1080/15592324.2024.2318513
Tahir Naqqash, Aeman Aziz, Muhammad Baber, Muhammad Shahid, Muhammad Sajid, Radicetti Emanuele, Abdel-Rhman Z Gaafar, Mohamed S Hodhod, Ghulam Haider
Plant growth-promoting rhizobacteria (PGPRs) have been utilized to immobilize heavy metals, limiting their translocation in metal contaminated settings. However, studies on the mechanisms and interactions that elucidate how PGPRs mediate Nickel (Ni) tolerance in plants are rare. Thus, in this study we investigated how two pre-characterized heavy metal tolerant isolates of Morganella morganii (ABT9 and ABT3) improve Ni stress tolerance in Arabidopsis while enhancing its growth and yield. Arabidopsis seedlings were grown for five weeks in control/Ni contaminated (control, 1.5 mM and 2.5 mM) potted soil, in the presence or absence of PGPRs. Plant growth characteristics, quantum yield, and antioxidative enzymatic activities were analyzed to assess the influence of PGPRs on plant physiology. Oxidative stress tolerance was quantified by measuring MDA accumulation in Arabidopsis plants. As expected, Ni stress substantially reduced plant growth (shoot and root fresh weight by 53.25% and 58.77%, dry weight by 49.80% and 57.41% and length by 47.16% and 64.63% over control), chlorophyll content and quantum yield (by 40.21% and 54.37% over control). It also increased MDA content by 84.28% at higher (2.5 mM) Ni concentrations. In contrast, inoculation with M. morganii led to significant improvements in leaf chlorophyll, quantum yield, and Arabidopsis biomass production. The mitigation of adverse effects of Ni stress on biomass observed in M. morganii-inoculated plants was attributed to the enhancement of antioxidative enzyme activities compared to Ni-treated plants. This upregulation of the antioxidative defense mechanism mitigated Ni-induced oxidative stress, leading to improved performance of the photosynthetic machinery, which, in turn, enhanced chlorophyll content and quantum yield. Understanding the underlying mechanisms of these tolerance-inducing processes will help to complete the picture of PGPRs-mediated defense signaling. Thus, it suggests that M. morganii PGPRs candidate can potentially be utilized for plant growth promotion by reducing oxidative stress via upregulating antioxidant defense systems in Ni-contaminated soils and reducing Ni metal uptake.
{"title":"Metal-tolerant <i>morganella morganii</i> isolates can potentially mediate nickel stress tolerance in Arabidopsis by upregulating antioxidative enzyme activities.","authors":"Tahir Naqqash, Aeman Aziz, Muhammad Baber, Muhammad Shahid, Muhammad Sajid, Radicetti Emanuele, Abdel-Rhman Z Gaafar, Mohamed S Hodhod, Ghulam Haider","doi":"10.1080/15592324.2024.2318513","DOIUrl":"10.1080/15592324.2024.2318513","url":null,"abstract":"<p><p>Plant growth-promoting rhizobacteria (PGPRs) have been utilized to immobilize heavy metals, limiting their translocation in metal contaminated settings. However, studies on the mechanisms and interactions that elucidate how PGPRs mediate Nickel (Ni) tolerance in plants are rare. Thus, in this study we investigated how two pre-characterized heavy metal tolerant isolates of <i>Morganella morganii</i> (ABT9 and ABT3) improve Ni stress tolerance in Arabidopsis while enhancing its growth and yield. Arabidopsis seedlings were grown for five weeks in control/Ni contaminated (control, 1.5 mM and 2.5 mM) potted soil, in the presence or absence of PGPRs. Plant growth characteristics, quantum yield, and antioxidative enzymatic activities were analyzed to assess the influence of PGPRs on plant physiology. Oxidative stress tolerance was quantified by measuring MDA accumulation in Arabidopsis plants. As expected, Ni stress substantially reduced plant growth (shoot and root fresh weight by 53.25% and 58.77%, dry weight by 49.80% and 57.41% and length by 47.16% and 64.63% over control), chlorophyll content and quantum yield (by 40.21% and 54.37% over control). It also increased MDA content by 84.28% at higher (2.5 mM) Ni concentrations. In contrast, inoculation with <i>M. morganii</i> led to significant improvements in leaf chlorophyll, quantum yield, and Arabidopsis biomass production. The mitigation of adverse effects of Ni stress on biomass observed in <i>M. morganii</i>-inoculated plants was attributed to the enhancement of antioxidative enzyme activities compared to Ni-treated plants. This upregulation of the antioxidative defense mechanism mitigated Ni-induced oxidative stress, leading to improved performance of the photosynthetic machinery, which, in turn, enhanced chlorophyll content and quantum yield. Understanding the underlying mechanisms of these tolerance-inducing processes will help to complete the picture of PGPRs-mediated defense signaling. Thus, it suggests that <i>M. morganii</i> PGPRs candidate can potentially be utilized for plant growth promotion by reducing oxidative stress via upregulating antioxidant defense systems in Ni-contaminated soils and reducing Ni metal uptake.</p>","PeriodicalId":94172,"journal":{"name":"Plant signaling & behavior","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10965111/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140208750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-31Epub Date: 2023-12-26DOI: 10.1080/15592324.2023.2294425
Marina Grinberg, Nikolay Ilin, Yulia Nemtsova, Fedor Sarafanov, Angelina Ivanova, Alexey Dolinin, Polina Pirogova, Vladimir Vodeneev, Evgeny Mareev
Alternating magnetic fields (MF) with Schumann resonance frequencies accompanied the development of living organisms throughout evolution, but today it remains unclear whether they can have a special biological effect in comparison with surrounding non-resonant frequencies. This work shows some stimulating effect of extremely low-frequency MFs on morphometric parameters and the activity of physiological processes in wheat (Triticum aestivum L.). It is shown that the MF effect is more pronounced for transient processes - photosynthesis reactions and changes in electrical potential caused by turning on light. For light-induced electrical reactions, the dependence of the severity of the effect on the frequency of the applied MF was demonstrated. It is shown that the most pronounced effect occurs in the 14.3 Hz field, which corresponds to the second harmonic of the Schumann resonance. The predominant sensitivity of signal-regulatory systems gives reason to assume the influence of MFs with Schumann resonance frequencies on the interaction of plants with environmental factors under conditions of a changed electromagnetic environment. Such conditions can occur, for example, with an increase in lightning activity caused by climate change, which serves as the basis for the generation of Schumann resonances, and with the development of artificial ecosystems outside the Earth's atmosphere.
{"title":"Response of photosynthesis and electrical reactions of wheat plants upon the action of magnetic fields in the Schumann resonance frequency band.","authors":"Marina Grinberg, Nikolay Ilin, Yulia Nemtsova, Fedor Sarafanov, Angelina Ivanova, Alexey Dolinin, Polina Pirogova, Vladimir Vodeneev, Evgeny Mareev","doi":"10.1080/15592324.2023.2294425","DOIUrl":"10.1080/15592324.2023.2294425","url":null,"abstract":"<p><p>Alternating magnetic fields (MF) with Schumann resonance frequencies accompanied the development of living organisms throughout evolution, but today it remains unclear whether they can have a special biological effect in comparison with surrounding non-resonant frequencies. This work shows some stimulating effect of extremely low-frequency MFs on morphometric parameters and the activity of physiological processes in wheat (<i>Triticum aestivum</i> L.). It is shown that the MF effect is more pronounced for transient processes - photosynthesis reactions and changes in electrical potential caused by turning on light. For light-induced electrical reactions, the dependence of the severity of the effect on the frequency of the applied MF was demonstrated. It is shown that the most pronounced effect occurs in the 14.3 Hz field, which corresponds to the second harmonic of the Schumann resonance. The predominant sensitivity of signal-regulatory systems gives reason to assume the influence of MFs with Schumann resonance frequencies on the interaction of plants with environmental factors under conditions of a changed electromagnetic environment. Such conditions can occur, for example, with an increase in lightning activity caused by climate change, which serves as the basis for the generation of Schumann resonances, and with the development of artificial ecosystems outside the Earth's atmosphere.</p>","PeriodicalId":94172,"journal":{"name":"Plant signaling & behavior","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10761032/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139041162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}