Carbohydrate Polymer Technologies and Applications最新文献_第8页

Cinnamaldehyde release behavior of Pickering emulsion synergistically stabilized by Sapindus mukorossi modified palygorskite and chitosan

IF 6.2 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymer Technologies and Applications

Pub Date : 2024-11-29 DOI: 10.1016/j.carpta.2024.100614

Aiping Hui , Bin Mu , Yongfeng Zhu , Peng Liu , Aiqin Wang

Most of the studies are committed to explore the natural green stabilizer or bio-stabilizer to design biocompatible Pickering emulsion, while few works focus on developing a dynamic interfacial regulatory effect for the slow-release of active molecules from Pickering emulsion. Herein, Pickering emulsion was fabricated for the controlled release of cinnamaldehyde, which was synergistically stabilized by palygorskite modified with the active ingredients leached from Sapindus mukorossi and chitosan. The results showed that the interfacial cross-linking between chitosan and cinnamaldehyde took place at the oil-water interface based on the formation of Schiff base, while the modified palygorskite was adsorbed onto the oil-water interface to co-stabilize Pickering emulsion. The simulated gastrointestinal experiment indicated that the emulsion was stable in simulated gastric fluid (SGF) of pH 1.5, and then destroyed in simulated intestinal fluid (SIF) of pH 6.8 to realize the continuous release of cinnamaldehyde, where the amount of chitosan played a vital role. Furthermore, the antibacterial test showed that the growth of Escherichia coli and Staphylococcus aureus was almost completely suppressed when the concentration of Pickering emulsion was 25 µL/mL. Therefore, this study developed a facile strategy to regulate the controlled release of cinnamaldehyde based on green Pickering emulsion.

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引用次数: 0

Bacterial cellulose nanofibers layer with water-floating, photothermal, bio- and electro-active features

IF 6.2 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymer Technologies and Applications

Pub Date : 2024-11-29 DOI: 10.1016/j.carpta.2024.100618

M. Jokar , M. Montazer , N. Hemmatinejad , M. Mahmoudi Rad

Chemical modification of bacterial cellulose (BC) nanofibers layer with high purity, crystallinity, and water-holding capacity with good mechanical properties helps with its application in various fields. Here, BC was modified with copper acetate (CA) and dopamine hydrochloride (DA) to synthesize copper-based nanoparticles and polydopamine on the BC to produce novel properties. The optimum sample was the one treated with (10 w/w%) copper acetate and (0.5 g/l) dopamine among copper acetate (1–10 w/w%) and dopamine (0.5–2.0 g/l) based on the antibacterial properties. FTIR-ATR spectra and FESEM images indicated well-deposited copper-based nanoparticles and DA on the surface of BC. The water contact angle increased from 18º on the raw BC to 89° on the modified BC. The BC color altered from cream to dark brown and the tensile strength increased by 206 %. The modified BC exhibited 99.8 and 99.9 % bacterial reduction against Escherichia coli and Staphylococcus aureus alongside 76.8 % cell viability. Further, DA-treated BC caused an increase in the temperature to 40 °C under IR light after 10 min as an indication of photothermal properties. Ultimately, the treated BC with CA and DA indicated anti-bacterial, photothermal, and water-floating properties with enhanced tensile strength.

{"title":"Bacterial cellulose nanofibers layer with water-floating, photothermal, bio- and electro-active features","authors":"M. Jokar , M. Montazer , N. Hemmatinejad , M. Mahmoudi Rad","doi":"10.1016/j.carpta.2024.100618","DOIUrl":"10.1016/j.carpta.2024.100618","url":null,"abstract":"<div><div>Chemical modification of bacterial cellulose (BC) nanofibers layer with high purity, crystallinity, and water-holding capacity with good mechanical properties helps with its application in various fields. Here, BC was modified with copper acetate (CA) and dopamine hydrochloride (DA) to synthesize copper-based nanoparticles and polydopamine on the BC to produce novel properties. The optimum sample was the one treated with (10 w/w%) copper acetate and (0.5 g/l) dopamine among copper acetate (1–10 w/w%) and dopamine (0.5–2.0 g/l) based on the antibacterial properties. FTIR-ATR spectra and FESEM images indicated well-deposited copper-based nanoparticles and DA on the surface of BC. The water contact angle increased from 18º on the raw BC to 89° on the modified BC. The BC color altered from cream to dark brown and the tensile strength increased by 206 %. The modified BC exhibited 99.8 and 99.9 % bacterial reduction against <em>Escherichia coli</em> and <em>Staphylococcus aureus</em> alongside 76.8 % cell viability. Further, DA-treated BC caused an increase in the temperature to 40 °C under IR light after 10 min as an indication of photothermal properties. Ultimately, the treated BC with CA and DA indicated anti-bacterial, photothermal, and water-floating properties with enhanced tensile strength.</div></div>","PeriodicalId":100213,"journal":{"name":"Carbohydrate Polymer Technologies and Applications","volume":"9 ","pages":"Article 100618"},"PeriodicalIF":6.2,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143093568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and characterisation of integrated wet-spun alginate-Moringa oleifera composite fibers for potential water purification

IF 6.2 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymer Technologies and Applications

Pub Date : 2024-11-28 DOI: 10.1016/j.carpta.2024.100620

Abimbola Oluwatayo Orisawayi , Krzysztof K. Koziol , Sameer S. Rahatekar

Ensuring access to safe drinking water requires effective materials and technologies to treat contaminated water. In this study, we developed sodium alginate fibre (SAlgF) and their composite fibres functionalised with pulverised Moringa oleifera (MoP) at concentrations of 0.5 %, 1 %, 4 %, and 8 % using the wet-spinning technique. Both SAlgF and MoP are biodegradable, offering eco-friendly alternatives to synthetic polymers in line with green manufacturing. The results showed significant improvements in the mechanical properties, with the 1 % MoP composite fibre exhibiting 6 times the strength of pure SAlgF in terms of ultimate tensile strength (UTS) and Young's modulus (YM). X-ray Diffraction (XRD) analysis revealed enhanced fibres interactions, while Fourier Transform Infrared Spectroscopy (FTIR) and Thermogravimetric Analysis (TGA) confirmed the successful incorporation of MoP into the alginate matrix and improved thermal stability. Furthermore, the result obtained from the Scanning Electron Microscopy-Energy Dispersive X-ray Spectroscopy (SEM-EDX) indicated morphological changes and the uptake of heavy metal ions when immersed into solutions containing Cu²⁺, Ni²⁺, and Cd²⁺. These findings demonstrate the potential of MoP-modified composite fibres for sustainable and cost-effective water treatment applications, particularly in developing countries.

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引用次数: 0

Modification of regenerated cellulose fibres by cork-derived suberin and the cutin fraction from grape skins 软木衍生的软木脂和葡萄皮中的果胶成分对再生纤维素纤维的改性作用

IF 6.2 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymer Technologies and Applications

Pub Date : 2024-11-22 DOI: 10.1016/j.carpta.2024.100613

Kaniz Moriam , Catarina Azevedo , Sara Fateixa , Fábio Bernardo , Herbert Sixta , Dmitry V. Evtuguin

Regenerated cellulose fibres from dissolving pulp are a versatile alternative to cotton fibres on the path to the sustainable textile industry. In this study, cellulose fibres obtained by the Ioncell-F® process (Ioncell fibres) were modified by adding 10 % (w/w) of suberin compounds isolated from cork (SUB) or a cutin fraction from grape skins (CUT) in the spinning dope. Although both SUB and CUT modified fibres revealed higher hydrophobicity than unmodified fibres, fibres doped with CUT showed better waterproof performance than those doped with SUB. This was explained by the better retention of CUT than SUB on the regenerated fibres and by the higher hydrophobicity of CUT. Differences in the strength properties of Ioncell fibres obtained by pilot-scale dry-jet wet spinning were related to their physical structure, whereas dirt repellence and susceptibility to enzymatic hydrolysis depended on the occurrence and amounts of retained CUT or SUB.

从溶解纸浆中提取的再生纤维素纤维是通往可持续纺织业道路上棉纤维的多功能替代品。在这项研究中，通过 Ioncell-F® 工艺获得的纤维素纤维（Ioncell 纤维）通过在纺纱涂料中添加 10 %（重量/重量）从软木中分离出来的单宁化合物（SUB）或从葡萄皮中分离出来的角质成分（CUT）进行改性。虽然经 SUB 和 CUT 改性的纤维都比未经改性的纤维具有更高的疏水性，但掺入 CUT 的纤维比掺入 SUB 的纤维具有更好的防水性能。这是因为 CUT 比 SUB 更好地保留在再生纤维上，而且 CUT 的疏水性更高。通过中试规模干喷湿纺获得的 Ioncell 纤维在强度特性上的差异与其物理结构有关，而防污性和对酶水解的敏感性则取决于所保留的 CUT 或 SUB 的存在和数量。

引用次数: 0

Carboxymethyl chitosan oligosaccharide enzymatic hydroxylates with reactive oxygen species scavenging and anti-inflammatory activity for topical treatment of skin photodamage 具有活性氧清除和抗炎活性的羧甲基壳聚糖低聚糖酶水解物，用于皮肤光损伤的局部治疗

IF 6.2 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymer Technologies and Applications

Pub Date : 2024-11-20 DOI: 10.1016/j.carpta.2024.100612

Ziming Zhu , Hui Li , Xiansen Lv , Yan Yang , Baoqin Han , Zhiwen Jiang

Skin photodamage caused by ultraviolet (UV) radiation is a major public health concern. UVB rays penetrate the epidermis, inducing oxidative stress and compromising essential cellular components. The demand for natural ingredients with properties such as moisturization, anti-inflammatory effects, antioxidant protection, and compatibility is increasing to combat the impact of photodamage on the skin. Herein, the carboxymethyl chitosan oligosaccharide (CM-COS) enzymatic hydroxylates were used on the topical application of skin photodamage for the first time. CM-COS, the degradation product of carboxymethyl chitosan, has high water solubility and multiple biological activities. Results showed that CM-COS promoted human epidermal cell (HaCaT) migration without causing cytotoxicity. In photodamaged HaCaT cells, CM-COS maintained cell viability and cytoskeletal integrity while inhibiting reactive oxygen species accumulation, apoptosis, and cell cycle arrest. CM-COS regulates gene expression related to cell cycle, oxidative stress, and inflammation in UVB-radiated HaCaT based on transcriptomic and qPCR data. In UVB-exposed mice, topical treatment of CM-COS significantly alleviated redness and scab formation and increased the moisture content of photodamaged skin. Histological analyses revealed reduced epidermal thickness and increased collagen I and collagen III deposition in photodamaged skin following CM-COS treatment. Additionally, gene expression related to the cell cycle, extracellular matrix, and inflammation were significantly activated by CM-COS based on transcriptomic and qPCR data. CM-COS modulated the levels of collagen and inflammation-related proteins. Collectively, the data confirm the therapeutic effect of CM-COS against skin photodamage and highlight the potential of natural marine oligosaccharides for treating skin diseases such as aging, wounds, and burns.

紫外线（UV）辐射造成的皮肤光损伤是一个主要的公共健康问题。紫外线穿透表皮，诱发氧化应激，损害细胞的基本成分。为了应对光损伤对皮肤的影响，人们对具有保湿、抗炎、抗氧化和兼容性等特性的天然成分的需求与日俱增。本文首次将羧甲基壳聚糖寡糖（CM-COS）酶羟化物用于皮肤光损伤的局部治疗。CM-COS 是羧甲基壳聚糖的降解产物，具有高水溶性和多种生物活性。研究结果表明，CM-COS 可促进人类表皮细胞（HaCaT）迁移，且不会产生细胞毒性。在光损伤的 HaCaT 细胞中，CM-COS 可维持细胞活力和细胞骨架完整性，同时抑制活性氧积累、细胞凋亡和细胞周期停滞。根据转录组和 qPCR 数据，CM-COS 可调节 UVB 辐射 HaCaT 细胞中与细胞周期、氧化应激和炎症有关的基因表达。在暴露于 UVB 的小鼠中，CM-COS 的局部治疗可明显减轻红肿和痂皮的形成，并增加光损伤皮肤的含水量。组织学分析表明，CM-COS 治疗后，光损伤皮肤的表皮厚度减少，胶原 I 和胶原 III 沉积增加。此外，根据转录组和 qPCR 数据，与细胞周期、细胞外基质和炎症有关的基因表达也被 CM-COS 显著激活。CM-COS 调节了胶原蛋白和炎症相关蛋白的水平。总之，这些数据证实了 CM-COS 对皮肤光损伤的治疗效果，并凸显了天然海洋寡糖在治疗皮肤疾病（如老化、伤口和烧伤）方面的潜力。

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引用次数: 0

Chitosan-based multimodal polymeric nanoparticles targeting pancreatic β-cells 靶向胰腺β细胞的壳聚糖基多模式聚合物纳米粒子

IF 6.2 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymer Technologies and Applications

Pub Date : 2024-11-19 DOI: 10.1016/j.carpta.2024.100610

Lorenzo Rossi , Cataldo Pignatelli , Krisztina Kerekes , Francesca Cadamuro , András Dinnyés , Felix Lindheimer , Jochen Seissler , Magdalena Lindner , Sibylle Ziegler , Peter Bartenstein , Yi Qiu , Judit Kovács-Kocsi , Zoltán Körhegyi , Magdolna Bodnár , Erika Fazekas , Eszter Prépost , Francesco Nicotra , Laura Russo

Multimodal in vivo imaging of pancreatic islets might improve monitoring of endocrine grafts upon implantation, helping clinical validation of new regenerative therapies based on the replacement of β-cells in type 1 diabetes affected patients. Herein, the generation of chitosan-based multimodal diagnostic nanoparticles (NPs) able to target β-cells is described. The NPs, composed of chitosan (CH) and γ-poly-glutamic-acid (γ-PGA) with different “clickable” functional groups were chemoselectively decorated at the surface with Exendin-4 (Ex4), a ligand of glucagon-like peptide 1 (GLP-1) β-cell receptors, and with a DOTA containing linker, to chelate diagnostic radioisotopes. Furthermore, the NPs were conjugated with IRDye®800CW for multispectral optoacoustic tomography (MSOT). The affinity of Ex4 decorated NPs towards GLP-1R was confirmed by competitive flow cytometry tests. The detectability of the NPs labeled with IRDye®800CW and Ex4 in MSOT experiments was demonstrated. In vivo biodistribution of Ex4 decorated NPs labelled with Ga-68 was studied with positron emission tomography (PET) experiments in mice. Specific binding to GLP-1 receptor expressing tissue was demonstrated in autoradiography experiments, showing potential of the multimodal NPs for specifically targeting β-cells.

胰岛的活体多模态成像可改善对内分泌移植物植入后的监测，有助于临床验证基于替代1型糖尿病患者β细胞的新型再生疗法。本文介绍了能靶向β细胞的壳聚糖基多模式诊断纳米粒子（NPs）的生成过程。这种由壳聚糖（CH）和γ-聚谷氨酸（γ-PGA）组成的 NPs 具有不同的 "可点击 "功能基团，其表面用胰高血糖素样肽 1（GLP-1）β 细胞受体配体 Exendin-4 (Ex4) 和含有 DOTA 的连接体进行化学选择性装饰，以螯合诊断放射性同位素。此外，NPs 还与 IRDye®800CW 共轭，用于多谱段光声断层扫描（MSOT）。竞争性流式细胞仪测试证实了经 Ex4 修饰的 NPs 对 GLP-1R 的亲和力。用 IRDye®800CW 和 Ex4 标记的 NPs 在 MSOT 实验中的可检测性也得到了证实。正电子发射断层扫描（PET）实验研究了用 Ga-68 标记的 Ex4 装饰 NPs 在小鼠体内的生物分布情况。自显影实验证明了与 GLP-1 受体表达组织的特异性结合，显示了多模式 NPs 特异性靶向 β 细胞的潜力。

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引用次数: 0

Removal of Lithium onto chitosan derivative using Taguchi method 利用田口方法在壳聚糖衍生物上去除锂

IF 6.2 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymer Technologies and Applications

Pub Date : 2024-11-18 DOI: 10.1016/j.carpta.2024.100611

Peter Osei Boamah , Jacqueline Onumah , Tayari Salifu , John Hendrick Essel , Benjamin Apam

This study aimed to remove lithium from a water-based mixture using a low molecular weight chitosan derivative (LMWCHT-LA-GLA). Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), and Nuclear Magnetic Resonance (NMR) spectroscopy were used to assess the sorbent. Lithium sorption capabilities were enhanced by LMWCHT-LA-GLA's functional groups OH, NH₂, and COOH. The initial concentration, sorbent dosage and pH had the biggest effects on sorption efficiency. At the ideal initial lithium content of 1 mg/L, sorbent quantity of 0.10 g and solution pH of 6, the percentage of removal was 62.75 %. The analysis of variance (ANOVA) revealed that the initial lithium concentration contributed the largest percentage (94.10 %) to the removal efficiency. Utilizing the Freundlich, Temkin and Langmuir sorption models, the experimental data were examined. The experimental data and the Langmuir isotherm agreed fairly well with a maximum sorption capacity of 7.00 mg/g. Pseudo-first- and pseudo-second-order kinetic models were used to analyze the kinetic data obtained at the optimal initial concentration. The pseudo-second-order model provided a good fit to the experimental data. Thermodynamic studies were used to determine the kind of lithium sorption. Overall results suggested that LMWCHT-LA-GLA is a promising sorbent for the removal of lithium from aqueous solution.

本研究旨在利用低分子量壳聚糖衍生物（LMWCHT-LA-GLA）去除水基混合物中的锂。傅立叶变换红外光谱（FTIR）、扫描电子显微镜（SEM）和核磁共振（NMR）光谱用于评估吸附剂。LMWCHT-LA-GLA的官能团OH、NH2和COOH增强了锂的吸附能力。初始浓度、吸附剂用量和 pH 值对吸附效率的影响最大。当理想的初始锂含量为 1 mg/L、吸附剂用量为 0.10 g、溶液 pH 值为 6 时，锂的去除率为 62.75%。方差分析（ANOVA）显示，初始锂浓度对去除率的影响最大（94.10%）。利用 Freundlich、Temkin 和 Langmuir 吸附模型对实验数据进行了检验。实验数据与 Langmuir 等温线相当吻合，最大吸附容量为 7.00 毫克/克。使用伪一阶和伪二阶动力学模型分析了在最佳初始浓度下获得的动力学数据。伪二阶模型与实验数据拟合良好。热力学研究用于确定锂的吸附类型。总体结果表明，LMWCHT-LA-GLA 是一种很有前途的从水溶液中去除锂的吸附剂。

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引用次数: 0

Anti-obesity functions of fucoidan conducted by bioinformatics and validation findings targeting of autophagy

IF 6.2 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymer Technologies and Applications

Pub Date : 2024-11-15 DOI: 10.1016/j.carpta.2024.100609

Jinkai Li , Xiaowei Wan , Yonghang Li , Ping Wang , Jian Chen , Weihua Jin , Jiaqi Liu

Autophagy, one of cell death mechanisms that lysosomes degrade endogenous cellular organelles and cytoplasm, is reported to regulate the development of obesity. Fucoidan, a sulfate-containing polysaccharide, is recognized for its anti-obesity effect. In our previous report, we presented the anti-obesity actions of fucoidan, however, the molecular mechanisms targeting autophagy remain unexplored. In this study, the purity, structure, and composition of compounds in fucoidan were characterized by using chemical analysis. Then, we conducted network pharmacology analysis to screen the pharmacological targets and to reveal the molecular mechanisms involved in anti-obesity action of fucoidan through targeting autophagy. Molecularly docked assay was used to further assess the spatial binding capability of fucoidan to target proteins. Our result identified core genes of fucoidan against obesity associated with autophagy. Functional enrichment analysis showed core genes involvement in metabolism, immunity, cell proliferation and differentiation, and kinase activity. Further pathway enrichment analysis chiefly comprised biological binding and immunity. Molecular docking data indicated that the key proteins resulted in potent affinities with fucoidan. Finally, in vitro adipocyte model and animal model were used to validate the preclinical findings that fucoidan suppressed adipocyte proliferation, reduced lipid deposition, induced autophagy, and inhibited adipogenic differentiation. Collectively, these bioinformatics and biochemical findings revealed the anti-obesity effects and mechanisms of fucoidan actions through targeting autophagy.

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引用次数: 0

Exploring the potential of mannan oligosaccharides in enhancing animal growth, immunity, and overall health: A review

IF 6.2 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymer Technologies and Applications

Pub Date : 2024-11-12 DOI: 10.1016/j.carpta.2024.100603

Neha Sharma

The growing demand for sustainable and efficient animal husbandry has driven significant interest in dietary supplements that enhance growth performance, immunity, and overall health. This review explores the promising potential of mannan oligosaccharides (MOS) as a functional dietary supplement in animal nutrition. Derived from yeast cell walls, MOS have garnered attention due to their prebiotic properties, which positively influence gut health by promoting beneficial microbiota and inhibiting pathogenic bacteria. Numerous studies demonstrate that MOS supplementation leads to improved growth rates, enhanced immune responses, and better nutrient utilization in various animal species, including poultry, swine, and aquaculture. This review synthesizes current research findings, highlighting beneficial effects of MOS such as modulating gut microbiota, enhancing intestinal morphology, and stimulating the immune system. Additionally, the review addresses the practical applications, optimal dosages, and potential challenges of incorporating MOS into animal diets. By elucidating the multifaceted benefits of MOS, this review underscores their role in advancing animal health and productivity, aligning with the industry's goals of achieving sustainable and efficient livestock production.

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引用次数: 0

Influence of consumption of unsaturated alginate oligosaccharides on the gut microbiota and the intestinal mucosal immunity homeostasis in immunocompromised mice 食用不饱和海藻酸寡糖对免疫缺陷小鼠肠道微生物群和肠粘膜免疫平衡的影响

IF 6.2 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymer Technologies and Applications

Pub Date : 2024-11-12 DOI: 10.1016/j.carpta.2024.100604

Zhaopeng Shen , Hua Yin , Lu Sun , Lu Chen , Jiandong Li , Xin Zhang , Mingyong Zeng , Xiaolu Jiang , Junhong Yu

Despite the well-known health benefits of unsaturated alginate oligosaccharides (UAOS), limited information exists on how they regulate the gut microbiota and intestinal mucosal immunity. In this study, UAOS was produced by alginate lyase degradation. Fourier transform infrared (FTIR), mass spectrometry (MS), and nuclear magnetic resonance (NMR) analyses showed that UAOS primarily consists of oligosaccharides, mainly pentamers, with a G/M ratio of 1.44 and unsaturated double bonds at the non-reducing end. UAOS exhibited good prebiotic effects; increased beneficial intestinal bacteria; improved the diversity, structure, and composition of the gut microbiota; and promoted the production of SCFAs. In particular, UAOS significantly increased the abundance of butyrate levels and their producing microbiota, such as Lachnospiraceae, Alloprevotella, and Butyicicoccus. Moreover, orally administered UAOS alleviated intestinal mucosal immunosuppression by upregulating the levels of the tight junction proteins occludin and ZO-1, enhancing the intestinal biochemical and immune barrier function by increasing levels of mucin-2 and SIgA, upregulating the CD4+/CD8+ ratio, regulating CD4+ T cell differentiation, and stimulated immune cytokine secretion and transcription factor production (T-bet/GATA-3). This process was related to TLR4/MyD88/NF-κB pathway. In summary, UAOS effectively regulates intestinal mucosal immune homeostasis by strengthening the intestinal barrier and regulating the intestinal microbiota and intestinal butyrate levels. Therefore, UAOS acts as a prebiotic and immune stimulator to improve host health.

尽管不饱和藻酸寡糖（UAOS）对健康的益处众所周知，但有关它们如何调节肠道微生物群和肠粘膜免疫的信息却很有限。在这项研究中，不饱和藻酸寡糖是通过藻酸酶降解产生的。傅立叶变换红外（FTIR）、质谱（MS）和核磁共振（NMR）分析表明，UAOS 主要由低聚糖组成，以五聚体为主，G/M 比为 1.44，非还原端为不饱和双键。UAOS 具有良好的益生作用，能增加肠道有益菌，改善肠道微生物群的多样性、结构和组成，促进 SCFAs 的产生。特别是，尿囊素明显增加了丁酸盐含量及其产生微生物群，如Lachnospiraceae、Alloprevotella和Butyicicoccus。此外，口服 UAOS 还能通过上调紧密连接蛋白 occludin 和 ZO-1 的水平缓解肠粘膜免疫抑制，通过提高粘蛋白-2 和 SIgA 的水平增强肠道生化和免疫屏障功能，上调 CD4+/CD8+ 比率，调节 CD4+ T 细胞分化，刺激免疫细胞因子分泌和转录因子产生（T-bet/GATA-3）。这一过程与 TLR4/MyD88/NF-κB 通路有关。总之，UAOS 可通过加强肠道屏障、调节肠道微生物群和肠道丁酸盐水平来有效调节肠道粘膜免疫平衡。因此，UAOS 可作为一种益生元和免疫刺激剂来改善宿主健康。

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引用次数: 0