Carbohydrate Polymer Technologies and Applications最新文献_第8页

p-Sulfonated calix[4]arene grafted on magnetic chitosan as efficient and recyclable multifunctional bio-nanocatalyst for various organic transformations 磁性壳聚糖接枝对磺酸杯芳烃作为多种有机转化的高效、可回收多功能生物纳米催化剂

IF 6.5 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymer Technologies and Applications

Pub Date : 2025-12-19 DOI: 10.1016/j.carpta.2025.101074

Saied Shafiei Navid, Rahman Hosseinzadeh, Mohammad Salehi

Biopolymers are abundant and sustainable materials that have various benefits, including reducing the development of secondary pollution because of their inherent environmental friendliness. For this reason, this study aims to prepare a chitosan-based magnetic nanocatalyst by grafting sulfonated calix[4]arene as a Brønsted acid moiety onto magnetic chitosan using isophorone diisocyanate as a linker. The prepared bio-based nanocatalyst, designated as SCA@MCS, was characterized using various analysis methods, including FT-IR, PXRD, TGA, SEM, EDS/MAPP, TEM, CHNS, VSM, and Zeta potential. The simultaneous presence of chitosan and p-sulfonated calix[4]arene endows SCA@MCS with both acidic and basic active sites, making it an effective multifunctional nanocatalyst. Consequently, the bio-based SCA@MCS was applied as a reusable heterogenous nanocatalyst for the efficient synthesis of various organic compounds, including dihydropyridine (89–98 %), dihydropyrimidine (82–98 %), dihydropyrrolone (89–95 %), and benzoate ester derivatives (98–99 %). The absence of toxic solvents, mild reaction conditions, simple work-up procedures, the use of a low amount of nanocatalyst, high reaction yields, and the reusability of the nanocatalyst are the key advantages of the introduced SCA@MCS magnetic nanocatalyst. These results demonstrate that the grafting of sulfonated calix[4]arene onto magnetic chitosan provides an efficient bio-based multifunctional nanocatalyst for the synthesis of various biologically active compounds and biodiesel production.

生物聚合物是一种丰富的可持续材料，由于其固有的环境友好性，具有减少二次污染等多种效益。为此，本研究旨在以异佛尔酮二异氰酸酯为连接剂，将磺化的杯状芳烃作为br / nsted酸段接枝到磁性壳聚糖上，制备壳聚糖基磁性纳米催化剂。采用FT-IR、PXRD、TGA、SEM、EDS/MAPP、TEM、CHNS、VSM和Zeta电位等多种分析方法对制备的生物基纳米催化剂SCA@MCS进行了表征。壳聚糖和对磺化杯芳烃的同时存在使SCA@MCS具有酸性和碱性活性位点，成为一种有效的多功能纳米催化剂。因此，生物基SCA@MCS被用作可重复使用的多相纳米催化剂，用于高效合成各种有机化合物，包括二氢吡啶（89 - 98%）、二氢嘧啶（82 - 98%）、二氢吡咯酮（89 - 95%）和苯甲酸酯衍生物（98 - 99%）。无有毒溶剂，反应条件温和，后处理程序简单，纳米催化剂用量少，反应收率高，纳米催化剂的可重复使用是SCA@MCS磁性纳米催化剂的主要优点。这些结果表明，在磁性壳聚糖上接枝磺化杯芳烃为合成各种生物活性化合物和生产生物柴油提供了一种高效的生物基多功能纳米催化剂。

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引用次数: 0

A synthetic 6-O-sulfated glycopolymer induces tissue iron accumulation via competitively inhibiting the hepcidin/FPN1 axis 合成的6- o -硫代糖共聚物通过竞争性抑制hepcidin/FPN1轴诱导组织铁积累

IF 6.5 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymer Technologies and Applications

Pub Date : 2025-12-19 DOI: 10.1016/j.carpta.2025.101073

Zhujie Xu , Yi Liu , Jingxiao Chen , Bangjun Wen , Youjia Xu

Highly sulfated heparin-like glycosaminoglycans (GAGs) have been closely linked to disrupted iron homeostasis, yet no studies have employed site-specific sulfation modifications of GAGs to develop macromolecular compounds capable of modulating iron metabolism. Based on preliminary research, our team designed and synthesized an glycopolymer with 6-O-sulfated GlcNAc. In vitro administration experiments demonstrated that 6-O-sulfated glycopolymer significantly inhibits hepcidin-mediated internalization and degradation of ferroportin 1 (FPN1) on macrophage membranes, reducing cellular iron storage— an effect superior to that of conventional HS. Following CY5-labeled 6-O-sulfated glycopolymer administration in vivo, fluorescence tracking revealed broad tissue distribution, including abdominal organs and cortical bone. Meanwhile, 6-O-sulfated glycopolymer markedly decreased serum hepcidin level, increased serum ferritin concentration, and induced significant iron accumulation in the liver and bone cortex, while results from HE staining of hepatic, splenic, and renal tissues confirmed its biological safety. Computational docking, surface plasmon resonance (SPR) assay, and cellular thermal shift assay-western blot (CETSA-WB) further demonstrated high-affinity binding between 6-O-sulfated glycopolymer and hepcidin. These findings establish the 6-O-sulfate group as the critical functional moiety in HS-mediated systemic iron regulation, where 6-O-sulfated glycopolymer competitively inhibits the hepcidin/FPN1 axis to activate macrophage iron efflux, ultimately promoting tissue iron accumulation.

高硫酸化的肝素样糖胺聚糖（GAGs）与铁稳态的破坏密切相关，但目前还没有研究利用特定位点的硫酸化修饰GAGs来开发能够调节铁代谢的大分子化合物。在前期研究的基础上，我们设计并合成了6- o -硫酸化GlcNAc的糖共聚物。体外给药实验表明，6- o -硫代糖共聚物显著抑制hepcidin介导的巨噬细胞膜上铁转运蛋白1 （FPN1）的内化和降解，减少细胞铁的储存，其效果优于常规HS。在体内给药cy5标记的6- o -硫代糖共聚物后，荧光跟踪显示广泛的组织分布，包括腹部器官和皮质骨。同时，6- o -硫代糖共聚物显著降低血清hepcidin水平，升高血清铁蛋白浓度，诱导肝脏和骨皮质明显的铁积累，肝、脾、肾组织HE染色结果证实其生物安全性。计算对接、表面等离子体共振（SPR）实验和细胞热移测定-western blot （CETSA-WB）进一步证明了6- o -硫酸化糖共聚物与hepcidin之间的高亲和力结合。这些发现证实了6- o -硫酸盐基团是hs介导的系统性铁调节的关键功能片段，其中6- o -硫代糖共聚物竞争性地抑制hepcidin/FPN1轴，激活巨噬细胞铁外排，最终促进组织铁积累。

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引用次数: 0

Charged carboxymethyl chitosan based CO2-philic adsorbents for enhanced CO2 capture and release via frequency using steel mesh electric polarization device at low temperature 利用钢网电极化装置在低温下通过频率增强二氧化碳捕获和释放的带电羧甲基壳聚糖基亲二氧化碳吸附剂

IF 6.5 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymer Technologies and Applications

Pub Date : 2025-12-18 DOI: 10.1016/j.carpta.2025.101072

Chun-Yao Huang , Feng-Yen Lin , Muhammad Iqbal , Wei-Song Hung , Chien-Hsing Lu , Jem-Kun Chen

Electric polarization (EP) facilitates charge separation within carbohydrate polymers under an electric field—a phenomenon that has not been thoroughly explored for CO₂ capture. To investigate this effect, carboxymethyl chitosan (CMCS)-based molecularly imprinted polymers (MIPs) were applied as a shell layer over polyacrylonitrile fibrous membranes (Pfm), creating a core–shell Pfm/MIP that formed amide-rich, CO₂-attractive binding sites. The Pfm/MIPs were positioned between two conductive mesh electrodes, where an alternating electric field was applied during CO₂ adsorption. Without EP, the CO₂ adsorption of Pfm/MIP was measured at 0.90 mmol/g. However, under an EP frequency of 300 kHz and 16 V, the dynamic adsorption increased markedly to 1.66 mmol/g. At elevated frequencies (e.g., 1200 kHz), the amine dipoles fail to synchronize with the rapid field oscillations, leading to dielectric loss and promoting CO₂ release from the imprinted sites. At a partial pressure of 0.15 bar CO₂, the selectivity for CO₂ over N₂ ranged from 65.5 to 67.4, while the purity of the recovered CO₂ stream was between 92.7% and 93.9%. The Pfm/MIP system demonstrated recyclability across 70 cycles of alternating EP frequencies between 300 and 1200 kHz. This approach highlights the role of charged adsorbents in modulating CO₂ adsorption and desorption behavior.

电场作用下，电极化（EP）促进了碳水化合物聚合物内的电荷分离——这一现象尚未被充分研究用于CO₂捕获。为了研究这种效应，将羧甲基壳聚糖（CMCS）为基础的分子印迹聚合物（MIPs）作为壳层应用于聚丙烯腈纤维膜（Pfm）上，形成了核-壳Pfm/MIP，形成了富含酰胺的、具有CO 2吸引力的结合位点。Pfm/MIPs被放置在两个导电网状电极之间，在CO₂吸附过程中施加交变电场。在不加EP的情况下，Pfm/MIP的CO₂吸附量为0.90 mmol/g。而在EP频率为300 kHz，电压为16 V时，动态吸附显著增加至1.66 mmol/g。在较高的频率下（例如，1200 kHz），胺偶极子不能与快速场振荡同步，导致介电损耗并促进co2从印迹位点释放。在0.15 bar CO₂的分压下，CO₂对N₂的选择性为65.5 ~ 67.4，而回收的CO₂流的纯度为92.7% ~ 93.9%。Pfm/MIP系统在300至1200 kHz的交替EP频率下进行了70次循环，证明了其可回收性。这种方法强调了带电吸附剂在调节CO₂吸附和解吸行为中的作用。

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引用次数: 0

ROS-Responsive Iodine-Embedded Cyclodextrin Nanoplatform for Multipurpose Periodontitis Therapy 多用途牙周炎治疗的ros响应碘包埋环糊精纳米平台

IF 6.5 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymer Technologies and Applications

Pub Date : 2025-12-17 DOI: 10.1016/j.carpta.2025.101070

Siyu He , Huipeng Xu , Siwen Wang , Xintao Chen , Yunxi Li , Wuzhen Ma , Xiaohong Ren , Li Wu , Yanling Xue , Jiwen Zhang

Periodontitis, an inflammatory disorder driven by microbial dysbiosis, is featured as elevated reactive oxygen species (ROS) and dysregulated inflammation, leading to tissue destruction and alveolar bone resorption. A promising therapeutic approach involves antimicrobial actions with ROS neutralization. Addressing the clinical limitation of elemental iodine (I₂) to topical use, we engineered a ROS-scavenging covalent cyclodextrin framework (CCF) embedding I₂ to confer dual functionality for periodontitis treatment. Initially, potassium iodide cyclodextrin metal-organic framework (KI-CD-MOF) was synthesized, which was then cross-linked with oxalyl chloride to introduce a ROS-scavenging peroxalate ester bond. Simultaneously, the iodide ions in KI-CD-MOF were oxidized to I₂, which was integrated throughout the framework. The resulting I₂-CCF particles had a cubic morphology (300–500 nm) and an I₂ content of 1.03±0.45 %, exhibiting an outstanding biosafety profile, strong bactericidal effectiveness, and significant ROS elimination capability. In vitro, I₂-CCF downregulated the expression of pro-inflammatory factors TNF-α and IL-1β, while promoting the secretion of anti-inflammatory markers IL-10 and Arg-1. Crucially, in a rodent periodontitis model, I₂-CCF effectively suppressed dental plaque accumulation, reduced periodontal inflammation, and limited alveolar bone resorption. This work establishes I₂-CCF as a multipurpose nanotherapeutic that concurrently targets the infectious and oxidative components of periodontitis, offering a potent alternative to conventional treatments.

牙周炎是一种由微生物生态失调引起的炎症性疾病，其特征是活性氧（ROS）升高和炎症失调，导致组织破坏和牙槽骨吸收。一种很有前途的治疗方法包括与ROS中和的抗菌作用。为了解决局部使用元素碘（I2）的临床局限性，我们设计了一种包埋I2的共价环糊精框架（CCF），以赋予治疗牙周炎的双重功能。首先合成碘化钾环糊精金属有机骨架（KI-CD-MOF），然后与草酰氯交联，形成清除ros的过草酸酯键。同时，KI-CD-MOF中的碘离子被氧化为I2， I2在整个框架中被整合。得到的I2- ccf颗粒具有立方形态（300-500 nm）， I2含量为1.03±0.45%，具有良好的生物安全性、强杀菌效果和显著的ROS消除能力。在体外，I2-CCF下调促炎因子TNF-α和IL-1β的表达，促进抗炎标志物IL-10和Arg-1的分泌。关键是，在啮齿动物牙周炎模型中，I2-CCF有效抑制牙菌斑积累，减少牙周炎症，限制牙槽骨吸收。这项工作建立了I2-CCF作为一种多用途的纳米治疗药物，同时针对牙周炎的感染性和氧化性成分，为传统治疗提供了一种有效的替代方案。

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引用次数: 0

Tuning the biological scaffolds’ performance by the combination of two antioxidant and antimicrobial chitosan derivatives 两种抗氧化和抗菌壳聚糖衍生物的组合调节生物支架的性能

IF 6.5 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymer Technologies and Applications

Pub Date : 2025-12-16 DOI: 10.1016/j.carpta.2025.101069

C. Muñoz-Núñez , A. Barco-Martín , K. Deshpande , D.S. Schmidt , L. González-García , S. Trujillo , A. Muñoz-Bonilla , M. Fernández-García

In this study novel polymeric materials based on chitosan (CS) were synthesized by chemically modifying CS with two bioactive moieties: eugenol and a compound containing a thiazolium group. These modifications aimed to impart antioxidant and antimicrobial properties to the matrix. Additionally, the scaffolds were reinforced with chitin nanowhiskers (Nw) to improve their mechanical strength and stability. Porous three-dimensional scaffolds were fabricated via the freeze-drying process, resulting in highly interconnected pore networks suitable for cell infiltration and nutrient transport. Biological characterization revealed that the incorporation of the two bioactive groups significantly enhanced the antioxidant activity and antimicrobial efficacy against both Gram-positive and Gram-negative bacteria to the scaffolds. Mechanical testing demonstrated that the Nw reinforcement increased scaffold stiffness and resilience without compromising porosity. In vitro biological assays using fibroblasts showed favorable cytocompatibility and promoted sustained cell proliferation over three weeks. Fluorescence microscopy confirmed fibroblast adhesion and morphological adaptation within the scaffold architecture. Additionally, the scaffolds were evaluated for their immunomodulatory effects using macrophage cultures, revealing a balanced immune response with reduced proinflammatory signaling, which is critical for successful integration and reduced fibrosis in vivo. These results indicate that those are promising candidates for tissue engineering and regenerative medicine applications.

在壳聚糖的基础上，用丁香酚和含噻唑基团的化合物对壳聚糖进行化学修饰，合成了新型高分子材料。这些修饰旨在赋予基质抗氧化和抗菌特性。此外，甲壳素纳米晶须（Nw）增强了支架的机械强度和稳定性。通过冷冻干燥工艺制备多孔三维支架，形成适合细胞浸润和营养物质运输的高度互联的孔隙网络。生物学特性表明，两种生物活性基团的掺入显著增强了支架对革兰氏阳性菌和革兰氏阴性菌的抗氧化活性和抗菌活性。力学测试表明，Nw加固在不影响孔隙率的情况下增加了支架的刚度和弹性。使用成纤维细胞进行的体外生物试验显示出良好的细胞相容性，并在三周内促进持续的细胞增殖。荧光显微镜证实了成纤维细胞的粘附和支架结构的形态适应。此外，使用巨噬细胞培养评估了支架的免疫调节作用，揭示了平衡的免疫反应，减少了促炎信号，这对于成功整合和减少体内纤维化至关重要。这些结果表明，这些是有希望的候选组织工程和再生医学应用。

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引用次数: 0

Valorizing date waste into functional bacterial nanocellulose with dual adsorption–antifouling properties for wastewater remediation 将枣泥废渣转化为具有双重吸附-防污性能的功能性细菌纳米纤维素用于废水修复

IF 6.5 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymer Technologies and Applications

Pub Date : 2025-12-16 DOI: 10.1016/j.carpta.2025.101071

Jahirul Ahmed Mazumder , Aniss Kedjour , Adiba Akram , Mussarat Tasleem , Fawzi Banat

The valorization of agro-industrial byproducts is crucial for establishing circular bioeconomies. This study presents a novel approach to produce bacterial nanocellulose (BNC) using date seeds (DS) and pomace (DP) as sustainable, low-cost carbon feedstocks for Komagataeibacter hansenii and examines whether feedstock chemistry alone can program the functionality of BNC. DP and DS extracts presented contrasting soluble profiles, which translated into differences in BNC yield and function. DP-BNC showed superior production and hydration (WUC = 552 ± 31 % vs 480 ± 25 % for DS-BNC) and retained the cellulose I structure. Spectroscopic studies indicated the in situ incorporation of hydrophilic motifs, and ζ-potential measurements at pH 7 revealed a negative surface charge (∼ −25.6 mV) of BNC. Furthermore, DP-BNC functioned as a highly effective biosorbent, achieving adsorption capacities of 4.62 ± 0.26 mg/g for the cationic dye Crystal Violet (CV) and 3.85 ± 0.20 mg/g for Methyl Orange (MO), with pseudo-second-order kinetics and Langmuir equilibria; capacities were retained at ∼52 % for CV and ∼48 % for MO till the fifth cycle. Antifouling assays demonstrated 41.4 % lower BSA adsorption and 93.1 % lower Escherichia coli colonization on DP-BNC compared to DS-BNC. This research establishes a pragmatic pathway for transforming low-value dates residues into a high-performance biomaterial.

农业工业副产品的增值对建立循环生物经济至关重要。本研究提出了一种利用枣籽（DS）和渣（DP）作为Komagataeibacter hansenii的可持续、低成本碳原料生产细菌纳米纤维素（BNC）的新方法，并研究了原料化学是否可以单独编程BNC的功能。DP和DS提取物具有不同的可溶性谱，这导致了BNC产量和功能的差异。DP-BNC表现出较好的产量和水合作用（WUC = 552±31%，而DS-BNC为480±25%），并保留了纤维素I的结构。光谱研究表明亲水性基序的原位结合，在pH 7下的ζ电位测量显示BNC的表面带负电荷（~ - 25.6 mV）。此外，DP-BNC作为一种高效的生物吸附剂，对阳离子染料结晶紫（CV）的吸附量为4.62±0.26 mg/g，对甲基橙（MO）的吸附量为3.85±0.20 mg/g，具有准二级动力学和Langmuir平衡；CV和MO的容量分别保持在~ 52%和~ 48%，直到第五个循环。防污实验表明，与DS-BNC相比，DP-BNC的BSA吸附量降低41.4%，大肠杆菌定植量降低93.1%。本研究建立了将低价值枣渣转化为高性能生物材料的实用途径。

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引用次数: 0

Starch based biodegradable packaging systems and their interactions with food components, shelf-life implications: A review 淀粉基可生物降解包装系统及其与食品成分的相互作用，对保质期的影响：综述

IF 6.5 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymer Technologies and Applications

Pub Date : 2025-12-13 DOI: 10.1016/j.carpta.2025.101067

Anand kumar , Saranyadevi S․ , Selva Kumar T․ , Shankar Neupane , Subhash V. Pawde , Shucheng Liu , Sadaqat Ali , Shuai Wei

Growing environmental concerns surrounding petroleum-based plastics have accelerated the development of starch-based packaging systems as sustainable and biodegradable alternatives. This review critically examines the structure-property relationships of starch films, structure, modifications, interactions with food components, and recent advancements aimed at enhancing their functional performance. Strategies such as chemical modifications, polymer blending, and developing starch-based film with proteins, nanocomposites significantly enhance the mechanical strength, barrier properties, and functional characteristics. The incorporation of natural additives, including essential oils and plant-derived extracts, increases the antimicrobial and antioxidant properties, extends the shelf-life of various food products. Recent advancements, such as the integration of biopolymers, reduce water vapor permeability while maintaining the mechanical integrity. Emerging technologies, including reactive extrusion and 3D printing, further support functionalized film fabrication and the valorization of agricultural waste streams. Despite these advances, challenges remain in terms of moisture sensitivity, industrial scalability, and cost-effectiveness. This review synthesizes current insights on starch-based films with their types, properties, performance enhancement strategies, food component interactions, through composition modifications, and sustainability considerations, providing a roadmap for future research toward multifunctional, intelligent, and commercially viable starch-based packaging solutions.

围绕石油基塑料的日益增长的环境问题加速了淀粉基包装系统作为可持续和可生物降解替代品的发展。本文综述了淀粉膜的结构-性能关系，结构，修饰，与食品成分的相互作用，以及旨在提高其功能性能的最新进展。化学改性、聚合物共混、开发淀粉基膜与蛋白质、纳米复合材料等策略显著提高了机械强度、屏障性能和功能特性。天然添加剂的加入，包括精油和植物提取物，增加了抗菌和抗氧化性能，延长了各种食品的保质期。最近的进步，如生物聚合物的集成，在保持机械完整性的同时减少了水蒸气的渗透性。新兴技术，包括反应挤出和3D打印，进一步支持功能化薄膜制造和农业废物流的增值。尽管取得了这些进步，但在湿度敏感性、工业可扩展性和成本效益方面仍然存在挑战。本文综述了淀粉基薄膜的类型、性能、性能增强策略、食品成分的相互作用、通过成分修改和可持续性考虑等方面的最新研究成果，为未来研究多功能、智能化和商业上可行的淀粉基包装解决方案提供了路线图。

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引用次数: 0

Beyond commercial hemostats: Montmorillonite-enriched alginate sponge for rapid hemostasis and wound infection prevention 超越商业止血：富含蒙脱石海藻酸盐海绵快速止血和伤口感染预防

IF 6.5 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymer Technologies and Applications

Pub Date : 2025-12-13 DOI: 10.1016/j.carpta.2025.101068

Amir Behzadnia , Maryam Ghassemi , Ali Rezaie , Soumayeh Amirsaadat , Parinaz Nezhad-Mokhtari , Zahra Akbari Moghaddam , Somayeh Alimohammadi , Roya Salehi , Seyedhosein Jarolmasjed

Despite advances in conventional hemostatic technologies, uncontrolled arterial bleeding and secondary infection remain clinical challenges and leading causes of trauma-induced fatalities. Hence, developing hemostatic materials that rapidly form resilient physical obstruction, trigger coagulation cascades, and combat bacterial infection is of importance. Herein, we engineered a multifunctional ultra-porous Alg/MMT sponge derived from alginate and enriched with montmorillonite (MMT) nanosheets serving as bioactive agents, utilizing gas-foaming, ionic crosslinking, and lyophilization processes. The sponge exhibited antibacterial effectiveness targeting S. aureus and E. coli, as evidenced by inhibition zone, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) assays. The fabricated Alg/MMT sponge demonstrated biocompatibility by maintaining high cell viability and promoting cell proliferation, as confirmed by MTT and DAPI assays. In-vitro evaluations revealed enhanced efficacy over EquiSpon® in blood clotting (9.15%), adherence of erythrocytes (94.50%) and platelets (87.30%), as well as clotting time of 19 s (P-value < 0.001). Through a femoral artery trauma model in rats, the Alg/MMT sponge delivered a hemostatic effect, reducing blood loss to 0.75 g and achieving hemostasis in 32.66 s—representing a 7.5-fold improvement compared to EquiSpon® (P-value < 0.001). Together, these characteristics highlight the Alg/MMT sponge as a reliable hemostatic agent for clinical environments.

尽管传统止血技术取得了进步，但不受控制的动脉出血和继发性感染仍然是临床挑战和创伤性死亡的主要原因。因此，开发能够迅速形成弹性物理阻塞、触发凝血级联和对抗细菌感染的止血材料是非常重要的。在此，我们设计了一种多功能超多孔Alg/MMT海绵，来源于海藻酸盐，并富含蒙脱土（MMT）纳米片作为生物活性剂，利用气体发泡，离子交联和冻干工艺。通过抑菌区、最小抑菌浓度（MIC）和最小杀菌浓度（MBC）的测定，证实了海绵对金黄色葡萄球菌和大肠杆菌的抑菌效果。MTT和DAPI实验证实，制备的Alg/MMT海绵通过维持高细胞活力和促进细胞增殖表现出生物相容性。体外评估显示，EquiSpon®在凝血（9.15%）、红细胞粘附（94.50%）和血小板粘附（87.30%）方面的疗效增强，凝血时间缩短19 s （p值<； 0.001）。通过大鼠股动脉创伤模型，Alg/MMT海绵具有止血作用，将失血量减少到0.75 g，在32.66 s内实现止血，与EquiSpon®相比，改善了7.5倍（p值<； 0.001）。总之，这些特点突出了Alg/MMT海绵作为临床环境中可靠的止血剂。

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引用次数: 0

Guar gum/Kappa carrageenan-based hydrogel reinforced with surface altered, curcumin@ZIF-8: Assessing its ability to accelerate wound closure 瓜尔胶/Kappa卡拉胶基水凝胶表面改变增强，curcumin@ZIF-8：评估其加速伤口愈合的能力

IF 6.5 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymer Technologies and Applications

Pub Date : 2025-12-12 DOI: 10.1016/j.carpta.2025.101066

Pooja Suvarna , Likhith K․ , Chinthana Chidananda , Supriya S. Bhatt , Manasa Nune , Goutam Thakur

This study reports the development of a bioresorbable hydrogel based on κ-carrageenan (KC) and guar gum (GG) reinforced with cerium-doped, curcumin-loaded ZIF-8 nanoparticles (Ce@CUR/ZIF-8) to enhance curcumin’s wound healing management. UV–Vis absorption spectra confirmed encapsulation of curcumin within ZIF-8. FESEM analysis revealed that the ZIF-8 particles had an average diameter of 157.6 ± 15.7 nm, which increased to 172.7 ± 12.8 nm following curcumin loading and cerium doping. The nanoparticles were uniformly dispersed within the KC–GG hydrogel matrix, which was systematically evaluated for its moisture-retention capacity, structural characteristics, mechanical strength, and sustained curcumin release profile. The compressive strength of the hydrogel increased from 0.0317 ± 0.99 MPa to 0.0547 ± 0.72 MPa upon addition of the nanocomposite, demonstrating improved mechanical integrity. Cytocompatibility was assessed using the MTT assay, which revealed 90% cell viability on day 5, confirming its biocompatibility. In vivo studies indicated that Ce@CUR/ZIF-8 promoted the greatest degree of wound closure, due to the synergistic antioxidant and anti-inflammatory activities of cerium and curcumin. Histopathological evaluations further revealed accelerated tissue regeneration characterized by enhanced fibroblast proliferation, neovascularization, and collagen deposition. Overall, KC–GG hydrogels integrated with Ce@CUR/ZIF-8 nanoparticles represent a promising platform for advanced wound-care applications.

本研究报道了一种生物可吸收水凝胶的开发，该水凝胶以κ-卡拉胶（KC）和瓜尔胶（GG）为基础，通过掺铈、负载姜黄素的ZIF-8纳米颗粒（Ce@CUR/ZIF-8）增强，以增强姜黄素的伤口愈合管理。紫外可见吸收光谱证实姜黄素包封在ZIF-8中。FESEM分析表明，ZIF-8粒子的平均直径为157.6±15.7 nm，在姜黄素负载和铈掺杂后，ZIF-8粒子的平均直径增加到172.7±12.8 nm。将纳米颗粒均匀地分散在KC-GG水凝胶基质中，系统地评估了其保湿能力、结构特征、机械强度和姜黄素的持续释放情况。加入纳米复合材料后，水凝胶的抗压强度由0.0317±0.99 MPa提高到0.0547±0.72 MPa，力学完整性得到改善。使用MTT法评估细胞相容性，第5天显示90%的细胞存活率，证实其生物相容性。体内研究表明Ce@CUR/ZIF-8促进伤口愈合的程度最大，这是由于铈和姜黄素的协同抗氧化和抗炎活性。组织病理学评估进一步显示，以增强成纤维细胞增殖、新生血管形成和胶原沉积为特征的组织再生加速。总的来说，KC-GG水凝胶与Ce@CUR/ZIF-8纳米颗粒的集成代表了一个有前途的先进伤口护理应用平台。

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引用次数: 0

Fabricating a chitosan-based human placenta extract–encapsulated elastic zonal-structured scaffold for osteochondral applications 基于壳聚糖的人胎盘提取物包封弹性带状结构骨软骨支架的制备

IF 6.5 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymer Technologies and Applications

Pub Date : 2025-12-08 DOI: 10.1016/j.carpta.2025.101064

Hossein Shaygani , Mohammad Shahverdi , Mohamadreza Soltani , Mohammad Rezaei , Sona Zare , Amir Shamloo , Kaivan Mohammadi

Articular cartilage is an avascular and multilayer tissue with limited self-regenerating properties. 3D printing offers a promising approach for fabricating intricate structures that closely replicate the architecture and functional properties of native cartilage. In this study, we fabricated a four-layer 3D-printed thermoplastic polyurethane (TPU) scaffold with varying porosities, ranging from the lowest porosity at the bottom to the highest at the top, to mimic the mechanical properties of cartilage layers from the deep zone to the transition zone. An injectable hydrogel consisting of chitosan (CS) and human placenta extract (HPE) was injected into the 3D-printed scaffold to provide a growth factor-loaded, extracellular matrix (ECM)-mimicking environment with suitable cytocompatibility, aiming to enhance the low cellular activity of the TPU scaffold. An electrospun layer was used as the superficial layer of the scaffold to replicate the longitudinal orientation of collagen fibers at the cartilage surface. Mechanical analysis demonstrated zonal strain distribution in the scaffold similar to native cartilage tissue. The antibacterial assay demonstrated the bactericidal effects of CS and HPE, showing an inhibition zone of (2.892 ± 0.103 mm). The MTT assay quantitatively evaluated the cellular functionality of scaffolds, demonstrating sustained cell viability over 7 days. These combined features make this scaffold design an exceptional candidate for cartilage tissue regeneration applications.

关节软骨是一种无血管的多层组织，具有有限的自我再生特性。3D打印为制造复杂的结构提供了一种很有前途的方法，可以紧密复制天然软骨的结构和功能特性。在这项研究中，我们制作了一个四层3d打印热塑性聚氨酯（TPU）支架，其孔隙率从底部的最低孔隙率到顶部的最高孔隙率不等，以模拟软骨层从深层区到过渡区的机械性能。将壳聚糖（CS）和人胎盘提取物（HPE）组成的可注射水凝胶注射到3d打印支架中，提供一个具有合适细胞相容性的生长因子负载的细胞外基质（ECM）模拟环境，旨在增强TPU支架的低细胞活性。电纺丝层被用作支架的表层，以复制软骨表面胶原纤维的纵向方向。力学分析表明，支架的带状应变分布与天然软骨组织相似。抑菌实验表明，CS和HPE均具有抑菌作用，抑菌带为（2.892±0.103 mm）。MTT实验定量评估了支架的细胞功能，显示了7天以上的持续细胞活力。这些综合特点使这种支架设计成为软骨组织再生应用的特殊候选者。

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引用次数: 0