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Phenotypic assessment of rice landraces for genetic variability and diversity studies under heat stress 热胁迫下水稻地方品种遗传变异性和多样性的表型评价
Pub Date : 2022-03-31 DOI: 10.35709/ory.2022.59.1.4
Kakarla Lavanya, Suman K, A. Fiyaz, M. Chiranjeevi, R. Surender, A. Satya, P. Sudhakar, L. S. Rao
The present investigation was carried out to estimate the genotypic and phenotypic variability, heritability, genetic advance and divergence based on heat stress and yield associated traits using 48 landraces of rice grown during rabi 2016 and 2017 at ICAR-Indian Institute of Rice Research farm, Hyderabad. ANOVA revealed the existence of significant differences for all the traits under study. Phenotypic coefficient of variation (PCV) was found slightly elevated than the genotypic coefficient of variation (GCV). All the characters under study except time to maturity exhibited high heritability coupled with high genetic advance as a per cent of mean, which revealed the predominance of additive gene action in controlling these traits. Cluster analysis grouped the 48 landraces into ten distinct clusters. Cluster I consisted of one landrace, while cluster II and III had 9 landraces each. Clusters IV, V, VI, VII, VIII, IX and X had 6, 2, 3, 3, 1, 6 and 8 landraces respectively. Cluster I included one landrace Byama Jhupi which was superior for the trait number of grains per panicle. Highest single plant yield (Mugei), fertility percentage (Neta) and time of heading (Neta) were recorded by entries of cluster II and highest panicle number per plant was recorded by landrace (Chiiti Mutyalu) of cluster IX. The genotypes of cluster II, VIII and IX showed high spikelet fertility percentage. Hence the genetic resources of these clusters can be utilized in the breeding programmes for development of heat tolerant varieties. The study helped to understand the extent of genetic diversity among the genetic resources which serve as a treasure of highly useful traits which can be exploited in developing high yielding and stress tolerant varieties.
本研究利用icar -印度水稻研究所(icar - india Institute of rice Research) 2016年和2017年rabi期间种植的48个地方品种的水稻,评估了热胁迫和产量相关性状的基因型和表型变异、遗传力、遗传进步和分化。方差分析显示,所有研究性状均存在显著差异。表型变异系数(PCV)略高于基因型变异系数(GCV)。除成熟期外,其余性状均表现出较高的遗传力和较高的遗传超前率,表明加性基因作用在控制这些性状中占主导地位。聚类分析将48个地方品种分为10个不同的聚类。第一类由1只地方赛马组成,而第二类和第三类各有9只地方赛马。集群IV、V、VI、VII、VIII、IX和X分别有6个、2个、3个、3个、1个、6个和8个地方品种。集群1包括1个地方品种,每穗粒数较优。单株最高产量(Mugei)、育性率(Neta)和抽穗时间(Neta)记录在第二簇的条目中,单株最高穗数记录在第九簇的地方品种(Chiiti Mutyalu)中。集群II、VIII和IX的基因型表现出较高的小穗受精率。因此,这些集群的遗传资源可用于培育耐热品种的育种计划。该研究有助于了解遗传资源之间的遗传多样性程度,为培育高产耐寒品种提供宝贵的有用性状资源。
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引用次数: 0
Effect of irrigation and nitrogen management on water productivity and nutrient uptake of aerobic rice 灌氮管理对旱作水稻水分生产力和养分吸收的影响
Pub Date : 2022-03-31 DOI: 10.35709/ory.2022.59.1.12
S. Duary, K. Pramanik, Debasish Panda
Water and nitrogen fertilizer are the two important inputs for rice cultivation. A field experiment was carried out in split plot design, with three water management treatments, irrigation at (i) 75 % Cumulative Pan Evaporation (CPE), (ii) 100% CPE, (iii) 125% CPE in the main plot, and four nitrogen management strategies, (i) 100% nitrogen applied through chemical fertiliser, (ii) 75% through chemical fertiliser + 2.5 t ha-1 vermicompost, (iii) 50% through chemical fertiliser + 5.0 t ha-1 vermicompost, and (iv) 25% through chemical fertiliser +7.5 t ha-1vermicompost in subplot. The grain yield, straw yield, water productivity, nutrient uptake and economics of aerobic rice were considerably affected by irrigation and nitrogen management. Crops receiving irrigation at 125% CPE had the highest grain yield (3618 kg ha-1), which was comparable to crops receiving irrigation at 100 % CPE. Regardless of irrigation and nitrogen management, the straw yield of aerobic rice ranged from 4127 to 5092 kg ha-1. The highest total NPK uptake by grain was recorded with nitrogen at 100 % N through fertiliser (N1), which was significantly greater than what was obtained from other nitrogen treatments and irrigation management at 100 % CPE (I100) and 125 % CPE (I125) was significantly better than irrigation at 75 % CPE (I75). Irrigation management at 125 % CPE yielded the highest gross return, net return, and B: C ratio of aerobic rice, which was comparable to 100 % CPE and 100 % N through fertiliser, 75 % N through fertiliser + 2.5 t ha-1 vermicompost, and significantly higher than other nitrogen treatments.
水肥和氮肥是水稻栽培的两大重要投入品。采用分地块设计进行田间试验,采用3种水分管理处理,分别在主地块以(i) 75%累积蒸发皿蒸发量(CPE)、(ii) 100%累积蒸发量(CPE)、(iii) 125%累积蒸发量(CPE)灌溉,以及4种氮肥管理策略,即(i) 100%施氮于化肥,(ii) 75%施于化肥+ 2.5 t ha-1蚯蚓堆肥,(iii) 50%施于化肥+ 5.0 t ha-1蚯蚓堆肥,(iv) 25%施用化肥+7.5 t ha- 1蚯蚓堆肥。灌溉和氮肥管理对旱作水稻的籽粒产量、秸秆产量、水分生产力、养分吸收和经济效益均有显著影响。以125% CPE灌溉的作物籽粒产量最高(3618 kg hm -1),与以100% CPE灌溉的作物相当。在不考虑灌溉和氮肥管理的情况下,旱作水稻秸秆产量在4127 ~ 5092 kg hm -1之间。100%氮肥处理(N1)的籽粒氮磷钾总吸收量最高,显著高于其他氮肥处理,100% CPE (I100)和125% CPE (I125)的灌溉管理显著优于75% CPE (I75)。125% CPE的灌溉处理产生的好氧水稻的总收益、净收益和B: C比最高,与100% CPE + 100%氮肥、75%氮肥+ 2.5 t hm -1蚯蚓堆肥相当,显著高于其他施氮处理。
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引用次数: 1
Effect of seedling age on productivity and profitability in traditional rice landraces 苗龄对传统地方水稻生产效益的影响
Pub Date : 2022-03-31 DOI: 10.35709/ory.2022.59.1.13
A. Ashraf, Subbalakshmi Lokanadan
A field experiment was conducted at Tamil Nadu Agricultural University, with an objective to study the effect of seedling age on productivity and profitability in rice landraces under irrigated rice ecosystem. The experiment was laid out in split plot design with three replications. The treatments in main plot were three age of seedlings viz., 15, 20 and 25 days old seedlings and in sub plot eight rice landraces viz., Chandikar, Kuliyadichan, Kuruvaikalanjiyam, Norungan, Nootripathu, Black Kavuni, Red Kavuni, Njavara and CO(R) 50 (one high yielding variety). Observations were recorded on growth parameters such as plant height, tiller numbers and dry matter production. The yield attributes like productive tillers, total spikelet, filled grains, unfilled grains, spikelet sterility percentage, thousand grain weight, grain yield and straw yield were recorded at the time of harvest. From the study it was observed that 15 days old seedlings in all the eight rice landraces and one high yielding variety, recorded higher growth and yield attributes. In rice landraces, Red Kavuni recorded higher grain, straw yield and B:C ratio. Based on quality parameters, CO(R) 50 rice variety and landraces viz., Red Kavuni, Black Kavuni, Nootripathu, Chandikar recorded optimum values and were suitable for cooking purpose.
在泰米尔纳德邦农业大学进行了一项田间试验,目的是研究灌溉水稻生态系统下苗龄对地方水稻产量和盈利能力的影响。试验采用3个重复的分区设计。主区分别施用3个苗龄,即15、20和25日龄,子区分别施用8个地方品种,即昌迪卡、库利亚迪坎、库鲁瓦卡兰吉亚姆、诺龙根、努特里帕图、黑卡瓦尼、红卡瓦尼、恩贾瓦拉和CO(R) 50(1个高产品种)。记录了植株高度、分蘖数和干物质产量等生长参数。收获时记录有效分蘖数、总穗数、灌浆粒数、未灌浆粒数、小穗不育率、千粒重、籽粒产量和秸秆产量等产量属性。从研究中观察到,8个地方品种和1个高产品种的15天龄幼苗均有较高的生长和产量特征。在地方水稻品种中,红卡乌尼的籽粒、秸秆产量和B:C比均较高。基于质量参数,CO(R) 50的品种和地方品种,即红卡乌尼,黑卡乌尼,努特里帕图,Chandikar记录了最佳值,适合烹饪用途。
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引用次数: 1
Molecular characterization and varietal identification for multiple abiotic stress tolerance in rice (Oryza sativa L.) 水稻(Oryza sativa L.)耐多种非生物胁迫的分子特征及品种鉴定
Pub Date : 2022-03-31 DOI: 10.35709/ory.2022.59.1.7
A. Ali, B. R, Chennamsetti Lakshmi Naga Manikanta, S. Alex, Soni Kb, Viji Mm
Coexistence of two or more abiotic stresses is common in most of the rainfed lowland and upland rice growing areas of India and worldwide. Rice production under these conditions is not sustainable. Identification and development of multiple abiotic stress tolerant rice varieties are to be addressed. Here we tried to identify multiple abiotic stress tolerant varieties from a collection of earlier identified varieties for single stress and validated the known SSR markers for stress tolerance. Twenty rice genotypes were evaluated for individual abiotic stress such as drought, salinity and temperature initially and the tolerant three genotypes in each case were further evaluated for combination of stresses various physio-morphological and biochemical parameters were recorded. Among the genotypes evaluated for combination of stresses, PTB-7 was found to be tolerant for drought and salinity, Nagina-22 was tolerant against high temperature and salinity. However, the seeds did not germinate in the presence of all three stresses simultaneously. Twenty rice varieties viz., Chomala, MO-16, PTB-35, PTB-60, PTB-39, PTB-55, PTB-30, PTB-7, CRdhan307, Apo, Vyttila-3, Vyttila-4, Vyttila-5, Vyttila-6, Vyttila-7, Vyttila-8, Vyttila-9, Vyttila-10, Nagina-22 and NL-44 were further investigated using microsatellite markers to confirm the genotypic level of tolerance to combination of abiotic stresses. Rice genotypes were screened using 30 reported simple sequence repeat (SSR) markers that are linked to drought, salinity and temperature. Molecular marker analysis of rice genotypes also confirmed that RM8904 and RM1287 were associated with salinity tolerance, RM2612, RM6100 and RM5749 were linked to high temperature tolerant trait. Population analysis also revealed that there is five subpopulation among rice genotypes.
两种或两种以上的非生物胁迫共存在印度和世界上大多数雨养低地和旱地水稻种植区是常见的。在这种条件下,水稻生产是不可持续的。多种非生物抗逆性水稻品种的鉴定和开发是亟待解决的问题。在此,我们试图从早期鉴定的单一胁迫品种中鉴定出多个非生物抗逆性品种,并验证已知的抗逆性SSR标记。对20个水稻基因型分别进行了干旱、盐度和温度等非生物胁迫的初步评价,并对3个基因型在不同胁迫条件下的耐受性进行了综合评价。在组合胁迫评价中,PTB-7具有耐干旱和耐盐性,Nagina-22具有耐高温和耐盐性。然而,在这三种胁迫同时存在的情况下,种子并没有发芽。利用微卫星标记对Chomala、MO-16、PTB-35、PTB-60、PTB-39、PTB-55、PTB-30、PTB-7、CRdhan307、Apo、Vyttila-3、Vyttila-4、Vyttila-5、Vyttila-6、Vyttila-7、Vyttila-8、Vyttila-9、Vyttila-10、Nagina-22和NL-44等20个水稻品种进行基因型分析,以确定其抗非生物胁迫组合的基因型水平。利用已报道的30个与干旱、盐度和温度相关的SSR标记筛选水稻基因型。水稻基因型分子标记分析也证实,RM8904和RM1287与耐盐性状相关,RM2612、RM6100和RM5749与耐高温性状相关。群体分析还发现,水稻基因型之间存在5个亚群体。
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引用次数: 5
Effect of nutrient management strategies on productivity in rice-pigeon pea intercropping under drought prone rainfed ecology of Eastern India 印度东部干旱雨养生态下养分管理策略对稻鸽豌豆间作生产力的影响
Pub Date : 2022-03-31 DOI: 10.35709/ory.2022.59.1.9
BC Verma, NP Mandal, S. Roy, SM Prasad, A. Banerjee
An experiment was undertaken to compare the productivity of rice- sole with rice-pigeon pea intercropping under different nutrient management (inorganic, organic and integrated nutrient management) strategies in drought prone rainfed (upland) ecology. The experimental data showed that, rice equivalent yield (REY) (2.83 and 3.22 t ha-1 under rice sole and rice -pigeon pea respectively) and yield attributing characters (grain panicle-1 (73) and test weight (24.3g) were higher under inorganic nutrient management followed by the integrated nutrient management (T4) (2.25 t ha-1, 2.64 t ha-1, 64 and 22.7 g respectively). The yield gap between the inorganic and integrated nutrient management decreased over the three years, because, in the first year integrated nutrient management could not produce enough yield and difference was more which became narrow as this treatment produced comparatively more yield afterward. REY was more in rice-pigeon-pea system (varied from 1.13 to 3.22 t ha-1) over the rice-sole (1.01 to 2.83 t ha-1) across the nutrient management strategies. Thus it is evident that, rice- pigeon pea intercropping under integrated nutrient management in upland condition would be better choice in rainfed drought prone area to enhance farm income as well as system productivity.
在干旱旱作(旱地)生态系统中,比较了不同营养管理(无机、有机和综合营养管理)策略下稻-比目鱼和稻-鸽豆间作的生产力。试验数据表明,无机养分管理后综合养分管理(T4)(分别为2.25 t ha-1、2.64 t ha-1、64和22.7 g)的水稻同等产量(REY)和产量性状(穗数1(73)和试重(24.3g)较高。无机养分管理与综合养分管理的产量差距在3年内逐渐缩小,因为综合养分管理第一年产量不足,差异较大,但随着综合养分管理产量的增加,差异逐渐缩小。水稻-鸽子-豌豆系统的REY (1.13 ~ 3.22 t ha-1)高于水稻-比目鱼系统(1.01 ~ 2.83 t ha-1)。因此,旱作旱作地区采用综合营养管理的稻鸽豆间作是提高农业收入和提高系统生产力的较好选择。
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引用次数: 0
Weed control, yield and quality of small-grain scented rice as influenced by spacing and weed management in lower gangetic plains 恒河下游平原小粒香稻的杂草控制、产量和品质对间距和杂草管理的影响
Pub Date : 2022-03-31 DOI: 10.35709/ory.2022.59.1.11
A. Pal, M. Ghosh, D. Mazumdar, B. Patra, S. Banerjee
The effect of planting density and weed management on weed control, yield and quality of a non-Basmati type short-grained scented rice (cv. Radhunipagal) was studied at 'C' Block Farm of Bidhan Chandra Krishi Viswavidyalaya, Kalyani, Nadia, West Bengal. The field experiment was conducted in a split-plot design comprising 2 spacings (15 cm × 15 cm and 20 cm × 15 cm) in main plots and 6 weed control practices [weedy check, 2 hand weeding (HW), Butachlor as pre-emergence (PE) + HW, Butachlor (PE) + 2,4-D Na-salt as post-emergence (PoE), HW + 2,4-D Na-salt (PoE), mechanical weeding + HW) in sub-plots during rainy (kharif) season of 2013 and 2014. Mean number of total weeds in 1 m2 field was 94.3 and 118.8 at 28 and 56 days after transplanting (DAT); and weed control efficiency varied among 5 weed management practices over weedy check as: 49.0-67.3% at 28 DAT and 56.5-72.8% at 56 DAT. Close planting (15 cm × 15 cm) resulted in 9.1% greater grain yield (2.27 vs. 2.08 t/ha), higher net income (Rs. 23,700 vs. 20,900/ha) and B:C ratio (1.79 vs. 1.73) over wider spacing (20 cm × 15 cm). Manual weeding twice at 21 and 42 DAT recorded better weed control efficiency (67.3 and 72.8% at 28 and 56 DAT), grain yield (2.51 t/ha), milling recovery (65.9%) and net return (Rs. 25,900/ha). But chemical weed control [Butachlor (PE) + 2,4-D Na-salt (PoE)] was adjudged as less expensive option (Rs. 27,300/ha) for Radhunipagal rice with moderate grain yield (2.16 t/ha ), higher protein content (7.45%) and B: C ratio (1.90).
种植密度和杂草管理对非巴斯马蒂型短粒香稻杂草控制、产量和品质的影响。Radhunipagal)在西孟加拉邦纳迪亚Kalyani的Bidhan Chandra Krishi Viswavidyalaya的“C”区块农场进行了研究。田间试验采用分块设计,主样地采用2个间距(15 cm × 15 cm和20 cm × 15 cm),子样地采用6种除草措施[杂草检查、2次手除草(HW)、丁草胺(PE) + HW、丁草胺(PE) + 2,4- d钠盐(PoE)、HW + 2,4- d钠盐(PoE)、机械除草+ HW],分别于2013年和2014年雨季进行。移栽后28和56 d, 1 m2田间平均杂草总数分别为94.3和118.8株;5种杂草管理措施的杂草控制效率差异为:28个DAT时为49.0 ~ 67.3%,56个DAT时为56.5 ~ 72.8%。密植(15 cm × 15 cm)比宽间距(20 cm × 15 cm)增产9.1%(2.27吨/公顷比2.08吨/公顷),提高净收入(23,700卢比比20,900卢比/公顷)和B:C比(1.79比1.73)。在21日和42日进行两次人工除草,除草效率(28日和56日分别为67.3和72.8%)、籽粒产量(2.51吨/公顷)、碾磨回收率(65.9%)和净收益(25,900卢比/公顷)均有所提高。但对于产量适中(2.16 t/ha)、蛋白质含量较高(7.45%)、B: C比为1.90的Radhunipagal水稻而言,化学除草[丁草胺(PE) + 2,4- d钠盐(PoE)]成本较低(2.73万美元/公顷)。
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引用次数: 0
Screening of rice genotypes for tolerance to soil acidity and related nutritional constraints 水稻耐土壤酸性基因型筛选及相关营养制约
Pub Date : 2022-03-31 DOI: 10.35709/ory.2022.59.1.8
PB Saha, A. Jha, Shaun S Kumar, K. Prasad
A field experiment was conducted in the experimental farm of Zonal Agricultural Research Station, Dumka, Birsa Agricultural University, Ranchi, Jharkhand during kharif 2018 to evaluate location specific genotypes for tolerance to soil acidity and related nutrient constraints in rainfed medium low lands. The experimental findings have been interpreted in terms of grain yield, crop response (%) to lime, lime use efficiency (LUE), P and K-uptake by the crop. The experiment was laid out in split plot design in which fertilizer levels (two) [NPK (RD) i.e., @ (100:50:25) kg/ha and NPK (RD) + lime @3q/ha] were taken as main plots and rice genotypes (fourteen) as sub-plots. It was found that the grain yield and nutrient uptakes (P & K) by the crop were significantly influenced by lime (irrespective of vars.) and genotype differences (irrespective of lime levels). Significantly higher grain yield (55.20 q/ha), P-uptake (17.21kg/ha) and K-uptake (19.13kg/ha) were recorded with (NPK + Lime) (irrespective of vars.). The highest crop response to lime (26.9%) and lime use efficiency (4.74 kg grain /kg lime) were recorded with JKRH3333 and BINADHAN-75, respectively, whereas the maximum increase in P-uptake (19.1%) and K-uptake (33.0%) were obtained with BINADHAN-8 and PUP-223, respectively.
2018年秋季,在印度贾坎德邦兰齐Birsa农业大学Dumka区域农业研究站实验农场进行了田间试验,以评估雨养中低地对土壤酸度和相关养分限制的耐受性。试验结果用粮食产量、作物对石灰的反应(%)、石灰利用效率(LUE)、作物对磷和钾的吸收来解释。试验采用分畦设计,以施肥水平(2)[氮磷钾(RD),即@ (100:50:25)kg/ha和氮磷钾(RD) +石灰@3q/ha]为主畦,水稻基因型(14)为次畦。结果表明,石灰(与品种无关)和基因型差异(与石灰水平无关)对籽粒产量和养分吸收(P和K)有显著影响。氮磷钾+石灰处理的籽粒产量(55.20 q/ha)、吸磷量(17.21kg/ha)和吸钾量(19.13kg/ha)显著提高(与品种无关)。JKRH3333和BINADHAN-75处理对石灰的响应最高(26.9%),石灰利用率最高(4.74 kg kg粒/kg石灰),BINADHAN-8和PUP-223处理对磷素和钾素的吸收分别提高了19.1%和33.0%。
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引用次数: 0
Genetic analysis of parental lines and identification of heterotic hybrid combinations in rice (Oryza sativa L.) 水稻亲本遗传分析及杂种组合鉴定
Pub Date : 2022-03-31 DOI: 10.35709/ory.2022.59.1.1
Y. Mohan, L. Krishna, T. Singh, T. K. Babu, N. Varma, M. .. Ramana
Thirty hybrids along with their parents (B and R lines) and standard checks viz., MTU 1001 and 27P63 were evaluated for grain yield and related traits to study combining ability and heterosis in rice. The mean performance of the hybrids for most of the characters was found higher than that of parents. The analysis of variance revealed significant differences among lines, testers and hybrids for most of the characters studied. SCA variances were found lower than GCA variances for most of the characters and average degree of dominance was far from unity indicating the predominance of additive gene action for these traits. However, for effective bearing tillers and spikelet fertility the average degree of dominance was very near to unity inferring the predominance of non-additive genetic components for these traits. The gca effects revealed that the lines, RNR 25783 and RP 5980-109-12-9-27 had significant gca effects in desired direction for yield and yield attributing traits whereas the tester, CMS 59A was a good general combiner. SCA effects showed that the hybrid, JMS 13A × RNR 19361 was found to be good specific combiner for grain yield per plant and spikelet fertility. Similarly, the hybrids, CMS 59A × SYE 503-78-34-2, CMS 46A × WGL 347 and JMS 13A × RP 5980-109-12-9-27 were identified as good specific combiner for grain yield and yield contributing characters. Based on heterotic estimates, five hybrids viz., CMS 59A × RNR 25783, CMS 59A × WGL 14, JMS 13A × RNR 25783, CMS 59A × RNR 25776, JMS 13A × RP 5980-109-12-9-27 were identified as promising with more than 5% and 20% yield advantage over hybrid and varietal checks, respectively.
为研究水稻配合力和杂种优势,对30个杂交种及其亲本(B系和R系)和标准对照(MTU 1001和27P63)的产量及相关性状进行了评价。杂种多数性状的平均表现高于亲本。方差分析表明,所研究的大部分性状在系间、试间和杂交种间存在显著差异。大多数性状的SCA方差低于GCA方差,平均显性度也不一致,表明加性基因作用在这些性状中占优势。然而,在有效分蘖和小穗育性方面,平均优势度非常接近于一致,这表明这些性状的非加性遗传成分占主导地位。gca效应表明,RNR 25783和RP 5980-109-12-9-27在产量和产量归因性状的期望方向上具有显著的gca效应,而测试者CMS 59A是一个良好的一般组合。SCA效应表明,JMS 13A × RNR 19361是单株产量和小穗育性较好的特异性组合。同样,CMS 59A × SYE 503-78-34-2、CMS 46A × WGL 347和JMS 13A × RP 5980-109-12-9-27是籽粒产量和产量贡献性状较好的特定组合。在杂种优势评价基础上,鉴定出5个杂种,即CMS 59A × RNR 25783、CMS 59A × WGL 14、JMS 13A × RNR 25783、CMS 59A × RNR 25776、JMS 13A × RP 5980-109-12-9-27,分别比杂种和品种的产量优势大于5%和20%。
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引用次数: 0
Glycolate catabolic bypass pathway integration in rice could be effective in lowering photorespiratory rate with modulating starch content and grain quality 乙醇酸分解代谢旁路整合可通过调节淀粉含量和籽粒品质,有效降低水稻光呼吸速率
Pub Date : 2022-03-31 DOI: 10.35709/ory.2022.59.1.6
L. Nayak, D. Panda, G. K. Dash, M. Lal, P. Swain, M. Baig, Adarsh Kumar
Photorespiration, which is prevalent under higher temperature and arid conditions, significantly affects crop productivity by reducing yields up to 50% in C3 crops like rice under severe stress conditions. This is primarily attributed to a reduction in net photosynthetic rate (PN). Rice flag leaf photosynthesis is the primary supplier of sugar to the maturing spikelets after anthesis. This study evaluated the grain quality traits and starch content of the wild type (WT) and transgenic rice generated by introducing Escherichia coli (E. coli) glycolate catabolic pathway bypassed (GCPB) through agrobacterium mediated transformation. Leaf soluble protein, photosynthetic CO2 assimilation rate, leaf non-structural carbohydrate content, grain quality traits such as hulling and milling percentages, head rice recovery, water uptake, volume expansion, alkali spreading value, gel consistency, grain breadth, grain starch content and amylose content were affected to a great extent in GCPB transgenic plants (T4). This study indicates the possible role of photorespiratory bypass mechanism in the regulation of source-sink communication, starch biosynthesis and grain quality in rice.
在高温和干旱条件下普遍存在的光呼吸作用,在严重的胁迫条件下会使C3作物(如水稻)的产量降低50%,从而显著影响作物生产力。这主要归因于净光合速率(PN)的降低。水稻旗叶光合作用是开花后成熟小穗的主要供给者。本研究通过农杆菌介导的乙醇酸分解代谢途径(GCPB)转化,对野生型(WT)和转基因水稻的籽粒品质性状和淀粉含量进行了研究。GCPB转基因植株(T4)的叶片可溶性蛋白、光合CO2同化率、叶片非结构性碳水化合物含量、籽粒剥皮率和碾碎率、抽穗回收率、吸水率、体积膨胀率、碱扩散值、凝胶稠度、粒宽、籽粒淀粉含量和直链淀粉含量等品质性状均受到较大影响。本研究揭示了光呼吸旁路机制在水稻源库通讯、淀粉生物合成和籽粒品质调控中的可能作用。
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引用次数: 0
Study of gene action for yield and quality traits in rice (Oryza sativa L.) 水稻产量和品质性状的基因作用研究
Pub Date : 2022-03-31 DOI: 10.35709/ory.2022.59.1.2
Sreelakshmi Ch, R. P
The present experiment was conducted to study the gene action, heterosis, inbreeding depression and heritability for different yield and quality traits in the cross WGL 48684 x IR 36. Analysis of heterosis revealed that most of the traits show duplicate type of epistasis which is non- fixable and cannot be exploited through the general breeding procedures of self pollinated crops. Additive gene effect and complementary epistasis plays a lead role for plant height, number of ear bearing tillers per plant, kernel breadth and amylase content and they can easily be exploited for developing fixed lines. Significant inbreeding depression was found for ear bearing tillers per plant, SPAD Chlorophyll Meter Reading (SCMR), harvest index, grain yield, head rice recovery and kernel elongation ratio. Therefore, few cycles of recurrent selection followed by pedigree method would be effective and useful to utilize all types of gene effects by maintaining considerable heterozygosity through mating of selected plants in early segregating generations.
本试验研究了WGL 48684 × IR 36不同产量和品质性状的基因作用、杂种优势、近交抑制和遗传力。杂种优势分析表明,大部分性状表现为重复型上位性,这是不固定的,不能通过自花授粉作物的一般育种程序加以利用。加性基因效应和互补上位效应在株高、单株实穗分蘖数、籽粒宽度和淀粉酶含量等性状上起主导作用,易于开发固定品系。单株穗分蘖数、SPAD叶绿素读数(SCMR)、收获指数、籽粒产量、抽穗率和籽粒伸长率均出现显著的近交系下降。因此,通过选择的植物在早期分离代中进行交配,保持相当大的杂合度,从而利用所有类型的基因效应,采用家系法进行循环选择是有效和有用的。
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ORYZA- An International Journal on Rice
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