Time-temperature indicators (TTIs) are widely fabricated from color-sensing components with polydiacetylene (PDA) and metal oxide nanoparticles (MO-NPs) film formation as the core-shell structure. The influence of different types of MO-NPs such as zinc (ZnO-NPs), silicon (SiO-NPs), silver (AgO-NPs), and titanium (TiO-NPs) was investigated by color-changing sensors on PDA solutions and chitosan-polyvinyl alcohol (PVA) films. The PDA and chitosan/PVA solutions were formed by adding different MO-NPs, to reveal their diverse properties. The TiO-NPs/PDA film, as the core-shell structure, combined with the carboxylic groups and interacted with PDA. A thin PDA shell was formed on the TiO-NPs that enlarged the surface area and allowed more free rotation of the PDA side chains for TiO-NPs/PDA. As a result, the core-shell structure showed improved sensitivity in color changing (blue to red at 35°C within 96 h), reflected by a higher color response (%CR), corresponding to the shifting of λmax in the absorption. The TiO-NPs/PDA-embedded chitosan/PVA film also showed color sensing improvement (purplish-blue to purplish orange at 35○C within 1 day). Thus, TiO-NPs/PDA-embedded chitosan/PVA films showed potential as TTI devices for tracking product quality and temperature during storage.