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A Retrospective Clinical Study Of Factors Affecting Tetanus 破伤风影响因素的回顾性临床研究
Pub Date : 2008-12-31 DOI: 10.5580/1d03
L. Ramachandra, K. Shobha, P. Kannan
Tetanus is a devastating disease of muscle spasm and autonomic instability with a high mortality. Despite being easily preventable with a highly effective vaccine, tetanus remains a significant source of morbidity and mortality worldwide . Our aim of the study was to review the demographic and clinical presentations of tetanus and to study the route of entry, immunization status and outcome of the disease. This study included all cases diagnosed as tetanus in a period of 6 years from 1 January 2000 to 31 December 2005. Data of the patients was retrospectively collected from the Medical Records Department of Kasturba medical college hospital, Manipal. The data included details of the patients and clinical findings. The maximum incidence was noted in the age group above 30yrs of age and males were more than females. Incidence among patients of various occupations was higher among those involved in manual work farmers (66.66%) Common clinical manifestations included trismus with the common site of injury was lower Limb. Mortality was found to be higher with increase in severity of the disease and short Incubation period. Poor adherence to immunization schedule in adult population,low level of care administered for minor injuries by the primary health care providers, without keeping in mind the possibility of tetanus and lack of awareness among general public about the immunization schedule and the disease, was probably the reason for a higher incidence of tetanus in this country compared to the west. Severe tetanus had a higher mortality than moderate tetanus. The mortality of patients with tetanus remained phenomenally high (23.33%), the reason for which had to be elucidated with further prospective studies.
破伤风是一种肌肉痉挛和自主神经不稳定的毁灭性疾病,死亡率很高。尽管用高效疫苗很容易预防,但破伤风仍然是全世界发病率和死亡率的一个重要来源。我们的研究目的是回顾破伤风的人口统计学和临床表现,并研究该疾病的进入途径、免疫状况和结局。这项研究包括2000年1月1日至2005年12月31日6年间诊断为破伤风的所有病例。患者资料回顾性收集自马尼帕尔Kasturba医学院医院病历部。这些数据包括患者的详细信息和临床表现。发病率以30岁以上年龄组最高,男性多于女性。不同职业的患者发病率以体力劳动者较高(66.66%),常见临床表现为牙关,损伤部位多为下肢。死亡率随疾病严重程度的增加和潜伏期的缩短而增加。成年人对免疫接种计划的遵守程度较低,初级卫生保健提供者对轻伤的护理水平较低,不考虑破伤风的可能性,公众对免疫接种计划和疾病缺乏认识,这些可能是该国破伤风发病率高于西方的原因。重症破伤风的死亡率高于中度破伤风。破伤风患者的死亡率仍然很高(23.33%),其原因有待进一步的前瞻性研究来阐明。
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引用次数: 6
Chronoimmunomodulation of melatonin on bactericidal activity of human blood phagocytes 褪黑素对人血液吞噬细胞杀菌活性的时序免疫调节作用
Pub Date : 2008-12-31 DOI: 10.5580/7fd
E. L. França, Alfredo Pereira Junior, S. L. Oliveira, A. Honório-França
In this work we studied the chronoimmunomodulation effect of melatonin on blood phagocytes. This cells stimulated with melatonin and incubated with EPEC presented enhanced superoxide. Mononuclear (MN) and polymorphonuclear (PMN) phagocytes stimulated with melatonin in the presence of bacteria showed enhanced superoxide release.EPEC killing by MN phagocytes stimulated with melatonin was time dependent. The highest bactericidal index were observed over the period of 60 and 120 minutes of incubation. PMN phagocytes stimulated with melatonin, independently of time, showed increased in the bactericidal activity. Interactions between melatonin and phagocytes support the hypothesis of pineal chronomodulation microbicidal processes.
本文研究了褪黑素对血液吞噬细胞的时间免疫调节作用。用褪黑激素刺激细胞和用EPEC孵育的细胞表现出增强的超氧化物。在细菌存在的情况下,褪黑激素刺激单核(MN)和多形核(PMN)吞噬细胞表现出增强的超氧化物释放。褪黑素刺激MN吞噬细胞杀灭EPEC具有时间依赖性。在60和120分钟的孵育期间观察到最高的杀菌指数。褪黑素刺激PMN吞噬细胞,与时间无关,显示出增加的杀菌活性。褪黑素和吞噬细胞之间的相互作用支持松果体时间调节杀微生物过程的假设。
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引用次数: 4
Detection of antimicrobial activity in accessory gland secretions of the virgin male red palm weevil, Rhynchophorus ferrugineus 红棕象鼻虫(Rhynchophorus ferrugineus)雄性处女副腺分泌物抗菌活性的检测
Pub Date : 2008-12-31 DOI: 10.5580/abd
Leenamma Joseph, V. S. Josekumar, P. V. George
Background: Bioactive molecules have been identified from accessory gland secretions in insects. Antibacterial properties are identified in secretions of insect reproductive system.Study design: Antibacterial screening of male accessory reproductive gland (MARG) secretions in red palm weevil, R. ferrugineus was conducted by disc diffusion sensitivity method against eight bacterial species.Result: Accessory gland secretions showed antibacterial property as growth inhibition zones in all bacteria used in this study.Conclusion: Antimicrobial property of MARG may be evolved to protect the gametes during mating since reproductive tract is also route of microbial invasion in insects.
背景:从昆虫的副腺分泌物中已鉴定出生物活性分子。昆虫生殖系统分泌物具有抗菌特性。研究设计:采用圆盘扩散敏感法对红棕榈象鼻虫(R. ferrugineus)雄性副生殖腺(MARG)分泌物进行抑菌筛选。结果:副腺分泌物对所有细菌均有抑菌作用,为生长抑制区。结论:生殖生殖道是昆虫体内微生物入侵的途径,其抗菌作用可能是为了保护配子。
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引用次数: 1
Bioconversion of cellulose into fermentable sugars by Saccharomyces cerevisiae cells for the production of ethanol using Cellulolytic fungi isolated from soil 利用从土壤中分离的纤维素分解真菌,酿酒酵母细胞将纤维素转化为可发酵糖以生产乙醇
Pub Date : 2008-12-31 DOI: 10.5580/1620
R. Devi, S. Shankar
The fungal species with cellulase activity was isolated from the soil using standard procedures. Two isolates were found to produce relatively high amount of cellulase. The production of cellulase by the two isolates was achieved in liquid shake culture containing Carboxymethyl cellulose as substrate. The filter paper activity and CMCase activity of the two isolates were also compared. The 9 day culture filtrate of the two isolates also exhibited highest activity. Ethanol production was checked in the media containing CMC as substrate after saccharification and fermentation by Saccharomyces cerevisiae cells. The ethanol yield was found to be maximum on the 9 day culture of both the isolates. Thus the current work deals with comparison of cellulase activity of the two isolates and alcohol production of two isolates by simultaneous saccharification and fermentation.
采用标准方法从土壤中分离出具有纤维素酶活性的真菌。发现两株菌株产生较高数量的纤维素酶。这两种菌株在以羧甲基纤维素为底物的液体摇培养中生产纤维素酶。比较了两株菌株的滤纸活性和CMCase活性。两株菌株9 d培养滤液也表现出最高的活性。在以CMC为底物的培养基中,通过酿酒酵母细胞的糖化和发酵,检验了乙醇的产量。两株菌株在培养9 d时乙醇产量最高。因此,目前的工作是比较两种菌株的纤维素酶活性和两种菌株同时糖化和发酵的酒精产量。
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引用次数: 3
Enterotoxigenic E.coli In Coimbatore Drinking Water 哥印拜陀饮用水中的产肠毒素大肠杆菌
Pub Date : 2008-12-31 DOI: 10.5580/1bd2
L. Growther, J. Hena, I. Sagayaraj, K. Kishore, V. Kumar
Water is one of the potential carriers of pathogenic microorganisms and can endanger health and life of humans and animals. About 50% of deaths (4.6 million) in children under 5 years of age occur due to diarrhoeal diseases caused by drinking polluted water. Kudoh and Zen-Yoji Studied 7 outbreaks of diarrhoea caused by Escherichia coli serotype O11 and O159, out of which two outbreaks were caused by contaminated water supply. In India, more than 70% of the epidemic emergencies are either waterborne or water related. Although a substantial amount of work has been carried out on common water-borne pathogens in India, unfortunately only a little information is available on the emerging waterborne pathogens. A regular surveillance of resource and drinking water is one of the major mainstays of containing dreaded and often fatal waterborne diseases.The use of antibiotics to combat these infections is a very common practice. The drug resistance displayed by Escherichia coli is indicative of indiscriminate use of antibiotics. This warrants the initiation of steps to prevent public health hazards. Resistance of Escherichia coli to antibiotics is not only an obstacle for the control of this infection but also poses a great threat to public health through transferable resistance determinant (R-factor) on enteric flora or other enterobacteria in man through direct colonization with resistant bacteria. The aim of the study was to isolate, serogroup and examine the antibiogram of Escherichia coli, present in drinking water sources in Coimbatore.
水是病原微生物的潜在载体之一,可危及人类和动物的健康和生命。约50%的5岁以下儿童死亡(460万)是由于饮用受污染的水引起的腹泻病造成的。Kudoh和Zen-Yoji研究了7起由大肠杆菌血清型O11和O159引起的腹泻暴发,其中2起暴发是由受污染的供水引起的。在印度,70%以上的流行病突发事件要么是水传播的,要么与水有关。虽然印度对常见的水媒病原体开展了大量工作,但不幸的是,关于新出现的水媒病原体的信息很少。对水源和饮用水的定期监测是控制可怕的、往往是致命的水传播疾病的主要支柱之一。使用抗生素来对抗这些感染是一种非常普遍的做法。大肠杆菌显示的耐药性表明滥用抗生素。因此,有必要采取措施,防止危害公众健康。大肠杆菌对抗生素的耐药不仅是控制该感染的障碍,而且还通过耐药菌的直接定植对肠道菌群或其他肠道细菌的可转移耐药决定因素(r因子)对公众健康构成巨大威胁。本研究的目的是分离大肠杆菌,血清组和检查抗生素谱,存在于哥印拜陀饮用水水源。
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引用次数: 2
The Hyperthrophy Of The Tibia Induced By The Volley-Ball . 排球运动引起的胫骨肥大。
Pub Date : 2008-12-31 DOI: 10.5580/1ca8
M. Tsili
In this work we studied the internal remodeling of the tibia, induced by the volleyball. It is shown that after a long time, the tibia of the athlete will be stren-gthened, that is it will be more stiff and less porous. The result is theoretical, based on a proposed theory of internal bone remodeling (Hegedus and Cowin, 1976) and comes in accordance with several clinical findings (Fehling et., al.,1995; Calbet et., al., 1999; Rittweger et., al.,2000; Ito et., al.,2001).In their studies, as the bone mineral den-sity (BMD) as the muscle strength indices (MBSI) of the tibia of the volleyball pla-yers were evaluated and they were significant higher, than the corresponding clini-cal findings of the normally active control subjects.
在这项工作中,我们研究了排球引起的胫骨内部重塑。由此可见,经过长时间的锻炼,运动员的胫骨会得到加强,也就是说,它会更僵硬,更少多孔。该结果是理论性的,基于提出的内部骨重塑理论(Hegedus和Cowin, 1976),并与一些临床发现相一致(Fehling等,al.,1995;Calbet et al., 1999;Rittweger等人,2000;伊藤等人,2001)。在他们的研究中,对排球运动员胫骨的骨矿物质密度(BMD)和肌肉力量指数(MBSI)进行了评估,发现它们明显高于正常运动的对照受试者的相应临床结果。
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引用次数: 1
Evaluation of antidermatophytic activity of ά-(2-hydroxy-2-methylpropyl)-ω-[2-hydroxy-3-methylbut-2-en-1-yl] polymethylene - the compound isolated from Caesalpinia bonducella (L.) Flem. α -(2-羟基-2-甲基丙基)-ω-[2-羟基-3-甲基丁-2-烯-1-基]聚亚甲基抗皮肤真菌活性评价Flem。
Pub Date : 2008-12-31 DOI: 10.5580/19cf
K. Sagar, G. Vidyasagar
A compound was isolated from ethyl acetate leaf extract of Caesalpinia bonducella (L.) Flem. and identified as ά-(2-hydroxy-2methylpropyl)-ω-[2-hydroxy-3-methylbut-2-en-1-yl] polymethylene by UV, IR, NMR, HPLC and GC/MS and evaluated for antifungal efficacy by Agar and broth dilution methods against clinical dermatophytes (viz., Trichophyton tonsurans, Trichophyton mentagrophytes, Trichophyton rubrum, Microsporum gypseum and Epidermophyton floccosum). In agar dilution method, T. tonsurans was inhibited only at 400 μg/ml concentration of the compound, whereas all others were inhibited even at low concentrations; optimum pH was 6.0; size of the colonies of the fungi deceased when inoculum size decreased from 10 to 10 spores/ml with increase with compound concentration from 100 to 400 μg/ml. In broth dilution method, mycelial dry weight of all test fungi decreased with the increase in the concentration of the compound; optimum pH was 6.5; decline in biomass of all fungi along with increase in concentration of the compound were observed which were significantly different (p<.05). The dermatophytes used in this study are the most common and widespread of this group and since, there is no scientific evidence to support the medical use of ά-(2-hydroxy-2-methylpropyl)-ω-[2-hydroxy-3-methylbut-2-en-1-yl] polymethylene, further studies are needed in order to elucidate the mechanism(s) of action of these compounds and their derivatives, as well as the antimicrobial activity against other microbial strain in particularly antibiotic resistant dermatophytes.
从山葵叶乙酸乙酯提取物中分离得到一个化合物。Flem。通过UV、IR、NMR、HPLC和GC/MS鉴定为α -(2-羟基-2-甲基丙基)-ω-[2-羟基-3-甲基-2-烯-1-基]聚乙烯,并通过琼脂和肉汤稀释法评价其对临床皮肤真菌(即:毛癣毛癣菌、mentagrophytes毛癣菌、rubrum毛癣菌、gypseum小孢子菌和絮状表皮癣菌)的抑菌效果。琼脂稀释法中,仅在浓度为400 μg/ml时,该化合物对褐斑单胞菌有抑制作用,而其他化合物在低浓度下均有抑制作用;最适pH为6.0;当接种量从10 ~ 10个孢子/ml,随着化合物浓度从100 ~ 400 μg/ml的增加,菌落死亡。在肉汤稀释法中,所有试验真菌的菌丝干重都随着化合物浓度的增加而降低;最适pH为6.5;随着化合物浓度的增加,真菌生物量呈下降趋势,差异有统计学意义(p< 0.05)。本研究中使用的皮肤植物是这一群体中最常见和最广泛的,由于没有科学证据支持α -(2-羟基-2-甲基丙基)-ω-[2-羟基-3-甲基丁-2-烯-1-基]聚乙烯的医疗用途,因此需要进一步研究以阐明这些化合物及其衍生物的作用机制,以及对其他微生物菌株的抗菌活性,特别是抗生素耐药皮肤植物。
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引用次数: 1
Production and evaluation of physicochemical properties of red pigment from Monascus purpureus MTCC 410 红曲霉MTCC 410红色素的制备及理化性质评价
Pub Date : 2008-12-31 DOI: 10.5580/d4a
Baljinder Kaur, Debkumar Chakraborty, Harbinder Kaur
Microbial pigments are secondary metabolites, which are produced during stationary or late log phase by a variety of microorganisms. M. purpureus MTCC 410 produces an extracellular red pigment in solid state fermentation on cooked autoclaved rice and MEA supplemented with ammonium nitrate. Pigment was extracted with ethanol and further quantified by taking OD at 500 nm. Anaerobic condition and low oxygen pressure inhibited Monascus growth as well as pigment production. Monascus pigment extracted from solid-state fermentation was less sensitive to light than from submerged fermentation. Purified red pigment is thermolabile over 70 oC heating and show a colour change from red to blackish when exposed to 100 oC for 15 min. Stability of the pigment at high temperature and low pH can be enhanced by modifying nitrogen source and substrate for culturing M. purpureus MTCC410.
微生物色素是次生代谢物,由多种微生物在静止或原木后期产生。M. purpureus MTCC 410在蒸熟的米饭和添加硝酸铵的MEA上固态发酵产生细胞外红色素。用乙醇提取色素,在500 nm处取OD进行定量。厌氧条件和低氧压力抑制了红曲霉的生长和色素的产生。固体发酵法提取的红曲红色素对光的敏感性低于浸没发酵法提取的红曲红色素。纯化后的红色素在70℃加热时具有热稳定性,在100℃下暴露15 min时颜色由红色变为黑色。通过改变培养M. purpureus MTCC410的氮源和底物,可以提高色素在高温和低pH下的稳定性。
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引用次数: 21
Anti-Microbial Susceptibility Patterns of Enterobacteriaceae Isolated From A Tertiary Care Unit In Gujarat 古吉拉特邦一家三级医疗机构分离的肠杆菌科细菌的抗菌敏感性模式
Pub Date : 2008-12-31 DOI: 10.5580/b2b
Jagruti C. Patel, J. Bhatt, V. A. Javiya, Kamlesh Patel
Objectives: Present study was undertaken to assess antibiotic susceptibility patterns of Enterobacteriaceae at a tertiary care hospital in Gujarat, India. Methods: Out of 276 culture positive samples, 154 samples of Enterbacteriaceae were examined and 11 different types of specimen were collected. Microbial sensitivity testing was done using disk diffusion test with Escherichia coli NCTC 10418 as per CLSI guidelines. Results: Highest Enterobacteriacae infections were found in urine followed by pus and sputum. Enterobacteriacae species demonstrated marked resistance against monotherapy of penicillins and cephalosporins. Combination of ampicillin, amoxicillin and third generation cephalosporins with sulbactam and monotherapy of amikacin showed higher sensitivity to Enterobacteriacae infections but maximum sensitivity was shown by carbapenems. Conclusions: Urinary tract infection was the most common hospital acquired infection. Co-administration of -lactamase inhibitor markedly expanded the anti-microbial sensitivity of penicillins and cephalosporins. Use of amikacin and carbapenems should be restricted to severe nosocomial infections to avoid rapid emergence of resistant strains.
目的:本研究旨在评估印度古吉拉特邦一家三级医院肠杆菌科细菌的抗生素敏感性。方法:276份培养阳性标本中,检测肠杆菌科154份,收集11种不同类型标本。微生物敏感性试验采用大肠杆菌NCTC 10418纸片扩散试验,按照CLSI指南。结果:尿中肠杆菌感染率最高,其次为脓和痰。肠杆菌对青霉素和头孢菌素的单一治疗表现出明显的耐药性。氨苄西林、阿莫西林和第三代头孢菌素联合舒巴坦和阿米卡星单药对肠杆菌感染的敏感性较高,但碳青霉烯类药物的敏感性最高。结论:尿路感染是最常见的医院获得性感染。同时给药-内酰胺酶抑制剂可显著提高青霉素类和头孢菌素类药物的抗菌敏感性。阿米卡星和碳青霉烯类药物的使用应仅限于严重的医院感染,以避免耐药菌株的迅速出现。
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引用次数: 12
Comparative Phylogenetics Approach for Discovering Alternative Source of Taxol 发现紫杉醇替代来源的比较系统发育方法
Pub Date : 2008-12-31 DOI: 10.5580/78
S. Kushwaha, P. Chauhan, M. Shakya
Bioprospecting is one of the prominent areas of research of commercial and valuable compounds, as it provides alternative sources of these compounds. One of the most persuasive methods for bioprospecting is through molecular phylogenetics analysis. Enzymes involved in the biosynthetic pathway of these compounds are considered as a base for bioprospecting. In this paper, an attempt has been made to find alternative sources for valuable compound taxol by comparative phylogenetics analysis using enzymes physiochemical data and sequence data. Dendogram was generated through physiochemical data whereas phylogenetic tree was generated through sequence data. Consequently after comparison four different organisms, Fungal BT2, OzoniumT2, Abies grandis and Ginkgo biloba, have been observed to be related to taxus plants both sequencially and physiochemically.
生物勘探是商业和有价值化合物研究的重要领域之一,因为它提供了这些化合物的替代来源。分子系统发育分析是生物勘探最有说服力的方法之一。参与这些化合物生物合成途径的酶被认为是生物勘探的基础。本文利用酶的理化数据和序列数据对紫杉醇进行系统发育比较分析,试图寻找有价值的化合物紫杉醇的替代来源。通过理化数据生成树突图,通过序列数据生成系统发育树。因此,通过对真菌BT2、OzoniumT2、冷杉(Abies grandis)和银杏(Ginkgo biloba) 4种生物的比较,发现它们在序列和生理化学上都与红豆杉属植物有亲缘关系。
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引用次数: 0
期刊
The Internet journal of microbiology
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