Pub Date : 2017-12-31DOI: 10.22435/hsji.v8i2.6051.
S. Siahaan, R. Handayani, N. Aryastami
Latar belakang: Tingkat kecacingan pada anak di Indonesia masih tinggi, untuk itu diperlukan pendekatan intervensi kesehatan masyarakat yang tepat. Tulisan ini bertujuan untuk meningkatkan pengetahuan, sikap dan perilaku (PSP) ibu dalam hal mengobati sendiri penyakit kecacingan dengan menggunakan Curcuma aeruginosa Roxb., bahan alam yang mudah ditemukan di Indonesia. Metode: Studi intervensi yang terdiri dari pengukur an terhadap tingkat PSP ibu (77) yang memiliki anak (80) usia 3 sampai 10 tahun, dimana dilakukan pemeriksaan kecacingan pada tinja anak-anak tersebut. Kemudian pada anak-anak yang terbukti menderita kecacingan diobati dengan minuman Curcuma dengan melibatkan ibu. Terhadap ibu juga diberi penyuluhan dan diukur kembali PSP nya terkait kecacingan dan pengobatan kecacingan dengan Curcuma. Tempat pengambilan sampel di Posyandu, desa Cibungbulang kabupaten Bogor. Hasil: PSP ibu meningkat setelah mereka diberikan penyuluhan kecacingan dan pengobatannya a.l pengetahuan bahwa kecacingan adalah penyakit menular (42,90% menjadi 84.40%) dan makanan yang kurang matang dan kotor menjadi jalan masauk bagi cacing kedalam tubuh ( 67,50% ke 92.20%), dan tanaman obat dapat digunakan untuk mengobati kecacingan (45.50% menjadi 79.20%). Kesimpulan: Edukasi kesehatan yang langsung melibatkan ibu untuk melakukan pengobatan kecacingan dengan Curcuma terbukti efektif untuk meningkatkan PSP sekaligus menyembuhkan kecacingan pada anak. Hasil studi menyarankan bahwa program pengobatan kecacingan pada anak dengan Curcuma dapat dilakukan pada program pengentasan kecacingan dan tidak perlu didahului dengan test pengujian kecacingan pada feces karena Curcuma terbukti efektif untuk pengobatan kecacingan, aman dan meningkatkan nafsu makan anak. Kata kunci: pengentasan kecacingan, Curcuma aeruginosa Roxb., anthelmintic, ibu dan anak Abstract Backgrounds: Worm infections in Indonesia are still high, it needs an appropriate public health intervention. This study aims to improve the knowledge, attitude and practice (KAP) of mothers about self-medication of worm infections using Curcuma aeruginosa Roxb., a natural plant easily found in Indonesia Methods: Study intervention involving the assessment of knowledge, attitude and practice of mothers (77) whose children (80) were feces tested to find out worm infections on their children. The children who confirmed suffering from worm infection were joined Curcuma treatment. Health education related to worm infections and its treatment with Curcuma were delivered to mothers. Then PSP of mothers were assessed again using the same questionnaires as before. Samples were collected from two Village Integrated Health Post in Cibungbulang village Bogor Municipality Results: Before-after intervention showed, the KAP of mothers increased after they got health education & information i.e. mothers knew that worm infections was a contagious disease 42.90% to 84.40%, mothers knew that dirty or poor cooked food is the way of worm g
{"title":"Improving the use of Curcuma aeruginosa Roxb. as anthelmintic for children in Bogor Regency","authors":"S. Siahaan, R. Handayani, N. Aryastami","doi":"10.22435/hsji.v8i2.6051.","DOIUrl":"https://doi.org/10.22435/hsji.v8i2.6051.","url":null,"abstract":"Latar belakang: Tingkat kecacingan pada anak di Indonesia masih tinggi, untuk itu diperlukan pendekatan intervensi kesehatan masyarakat yang tepat. Tulisan ini bertujuan untuk meningkatkan pengetahuan, sikap dan perilaku (PSP) ibu dalam hal mengobati sendiri penyakit kecacingan dengan menggunakan Curcuma aeruginosa Roxb., bahan alam yang mudah ditemukan di Indonesia. Metode: Studi intervensi yang terdiri dari pengukur an terhadap tingkat PSP ibu (77) yang memiliki anak (80) usia 3 sampai 10 tahun, dimana dilakukan pemeriksaan kecacingan pada tinja anak-anak tersebut. Kemudian pada anak-anak yang terbukti menderita kecacingan diobati dengan minuman Curcuma dengan melibatkan ibu. Terhadap ibu juga diberi penyuluhan dan diukur kembali PSP nya terkait kecacingan dan pengobatan kecacingan dengan Curcuma. Tempat pengambilan sampel di Posyandu, desa Cibungbulang kabupaten Bogor. Hasil: PSP ibu meningkat setelah mereka diberikan penyuluhan kecacingan dan pengobatannya a.l pengetahuan bahwa kecacingan adalah penyakit menular (42,90% menjadi 84.40%) dan makanan yang kurang matang dan kotor menjadi jalan masauk bagi cacing kedalam tubuh ( 67,50% ke 92.20%), dan tanaman obat dapat digunakan untuk mengobati kecacingan (45.50% menjadi 79.20%). Kesimpulan: Edukasi kesehatan yang langsung melibatkan ibu untuk melakukan pengobatan kecacingan dengan Curcuma terbukti efektif untuk meningkatkan PSP sekaligus menyembuhkan kecacingan pada anak. Hasil studi menyarankan bahwa program pengobatan kecacingan pada anak dengan Curcuma dapat dilakukan pada program pengentasan kecacingan dan tidak perlu didahului dengan test pengujian kecacingan pada feces karena Curcuma terbukti efektif untuk pengobatan kecacingan, aman dan meningkatkan nafsu makan anak. Kata kunci: pengentasan kecacingan, Curcuma aeruginosa Roxb., anthelmintic, ibu dan anak Abstract Backgrounds: Worm infections in Indonesia are still high, it needs an appropriate public health intervention. This study aims to improve the knowledge, attitude and practice (KAP) of mothers about self-medication of worm infections using Curcuma aeruginosa Roxb., a natural plant easily found in Indonesia Methods: Study intervention involving the assessment of knowledge, attitude and practice of mothers (77) whose children (80) were feces tested to find out worm infections on their children. The children who confirmed suffering from worm infection were joined Curcuma treatment. Health education related to worm infections and its treatment with Curcuma were delivered to mothers. Then PSP of mothers were assessed again using the same questionnaires as before. Samples were collected from two Village Integrated Health Post in Cibungbulang village Bogor Municipality Results: Before-after intervention showed, the KAP of mothers increased after they got health education & information i.e. mothers knew that worm infections was a contagious disease 42.90% to 84.40%, mothers knew that dirty or poor cooked food is the way of worm g","PeriodicalId":30666,"journal":{"name":"Health Science Journal of Indonesia","volume":"8 1","pages":"95-101"},"PeriodicalIF":0.0,"publicationDate":"2017-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44971590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-12-31DOI: 10.22435/HSJI.V8I2.7572.81-87
Ratna Budi Hapsari, D. Riana, E. Purwanto, N. Kandel, V. Setiawaty
Latar Belakang: Sistem kewaspadaan dini dan respons KLB (EWARS) adalah sistem surveilans sindrom berbasis web, yang diberlakukan di Indonesia sejak 2009, dimulai di dua provinsi. Untuk memberikan respons yang cepat terhadap sinyal yang terdeteksi pada EWARS, algoritma untuk diagnosis dan respons serta alat pemetaan kapasitas laboratorium dikembangkan. Penelitian ini bertujuan untuk menggambarkan kinerja penerapan EWARS dan untuk mengidentifikasi kekuatan dan kesenjangan EWARS sebagai sistem kewaspadaan dini dan respons KLB. Metode: Kinerja EWARS digambarkan dengan analisis data EWARS 2009-2011 di enam provinsi. Kekuatan dan kesenjangan EWARS diidentifikasi dengan melakukan penilaian umum di tiga provinsi terpilih dan penilaian kapasitas laboratorium di sembilan provinsi. Hasil: Kinerja EWARS cukup baik di Bali dan Lampung pada tahun pertama pelaksanaannya. Pada tahun 2010 dan 2011, kinerja EWARS di enam provinsi tetap baik. Sistem ini mudah digunakan dan bisa memberi informasi tentang peringatan dan pemetaan mingguan. Monitoring peringatan dengan menggunakan EWARS dapat digunakan sebagai alat evaluasi untuk melihat kualitas respon yang dilakukan oleh petugas kesehatan setempat atau Rapid Response Team (RRT). Meskipun konfirmasi laboratorium belum dilakukan untuk sebagian besar peringatan yang terdeteksi oleh sistem, pada umumnya, EWARS diterima dengan baik di Lampung, Bali, dan Kalimantan Selatan, dan memberi manfaat untuk meningkatkan kinerja pada fungsi peringatan dini. Kesimpulan: EWARS adalah kesempatan untuk memperkuat sistem surveilans yang berkelanjutan dan sensitif. Sistem ini diterima dengan baik karena mudah digunakan dan meningkatkan kinerja peringatan dini walapun masih perlu penguatan laboratorium. Kata kunci: Sistem Kewaspadaan Dini, Respons, Pemetaan, Surveilans berbasis Web Abstract Background: Early Warning Alert Response System (EWARS) is a web-based syndrome surveillance system, established in Indonesia since 2009, started in two provinces. In order to provide a prompt response of the signals detected on EWARS, the algorithms for diagnosis and response and the laboratory capacity mapping tool were developed. This study aims to describe performance of EWARS implementation and to identify the strengths and gaps of EWARS as a disease early warning and detection system. Methods: EWARS performance was described by analysis of the EWARS data 2009-2011 in six provinces. EWARS strengths and gaps were identified by conducting general assessment in three selected provinces and laboratory capacity assessment in nine provinces. Results : The performance of EWARS was quite good in Bali and Lampung at the first year of implementation. In 2010 and 2011, EWARS performance in six provinces was remain good. The system is easy to use and could give information on weekly alerts and mapping. Alert monitoring by using EWARS could be used as an evaluation tool to see the quality of response conducted by local health officers or Rapid Response Team (R
{"title":"Early Warning Alert and Response System (EWARS) in Indonesia: Highlight From The First Years of Implementation, 2009-2011","authors":"Ratna Budi Hapsari, D. Riana, E. Purwanto, N. Kandel, V. Setiawaty","doi":"10.22435/HSJI.V8I2.7572.81-87","DOIUrl":"https://doi.org/10.22435/HSJI.V8I2.7572.81-87","url":null,"abstract":"Latar Belakang: Sistem kewaspadaan dini dan respons KLB (EWARS) adalah sistem surveilans sindrom berbasis web, yang diberlakukan di Indonesia sejak 2009, dimulai di dua provinsi. Untuk memberikan respons yang cepat terhadap sinyal yang terdeteksi pada EWARS, algoritma untuk diagnosis dan respons serta alat pemetaan kapasitas laboratorium dikembangkan. Penelitian ini bertujuan untuk menggambarkan kinerja penerapan EWARS dan untuk mengidentifikasi kekuatan dan kesenjangan EWARS sebagai sistem kewaspadaan dini dan respons KLB. Metode: Kinerja EWARS digambarkan dengan analisis data EWARS 2009-2011 di enam provinsi. Kekuatan dan kesenjangan EWARS diidentifikasi dengan melakukan penilaian umum di tiga provinsi terpilih dan penilaian kapasitas laboratorium di sembilan provinsi. Hasil: Kinerja EWARS cukup baik di Bali dan Lampung pada tahun pertama pelaksanaannya. Pada tahun 2010 dan 2011, kinerja EWARS di enam provinsi tetap baik. Sistem ini mudah digunakan dan bisa memberi informasi tentang peringatan dan pemetaan mingguan. Monitoring peringatan dengan menggunakan EWARS dapat digunakan sebagai alat evaluasi untuk melihat kualitas respon yang dilakukan oleh petugas kesehatan setempat atau Rapid Response Team (RRT). Meskipun konfirmasi laboratorium belum dilakukan untuk sebagian besar peringatan yang terdeteksi oleh sistem, pada umumnya, EWARS diterima dengan baik di Lampung, Bali, dan Kalimantan Selatan, dan memberi manfaat untuk meningkatkan kinerja pada fungsi peringatan dini. Kesimpulan: EWARS adalah kesempatan untuk memperkuat sistem surveilans yang berkelanjutan dan sensitif. Sistem ini diterima dengan baik karena mudah digunakan dan meningkatkan kinerja peringatan dini walapun masih perlu penguatan laboratorium. Kata kunci: Sistem Kewaspadaan Dini, Respons, Pemetaan, Surveilans berbasis Web Abstract Background: Early Warning Alert Response System (EWARS) is a web-based syndrome surveillance system, established in Indonesia since 2009, started in two provinces. In order to provide a prompt response of the signals detected on EWARS, the algorithms for diagnosis and response and the laboratory capacity mapping tool were developed. This study aims to describe performance of EWARS implementation and to identify the strengths and gaps of EWARS as a disease early warning and detection system. Methods: EWARS performance was described by analysis of the EWARS data 2009-2011 in six provinces. EWARS strengths and gaps were identified by conducting general assessment in three selected provinces and laboratory capacity assessment in nine provinces. Results : The performance of EWARS was quite good in Bali and Lampung at the first year of implementation. In 2010 and 2011, EWARS performance in six provinces was remain good. The system is easy to use and could give information on weekly alerts and mapping. Alert monitoring by using EWARS could be used as an evaluation tool to see the quality of response conducted by local health officers or Rapid Response Team (R","PeriodicalId":30666,"journal":{"name":"Health Science Journal of Indonesia","volume":"8 1","pages":"81-87"},"PeriodicalIF":0.0,"publicationDate":"2017-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46597114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-12-31DOI: 10.22435/hsji.v8i2.6854.
Dyah Widiastuti, B. Ikawati, M. Martini, N. Wijayanti
Latar Belakang: Resistensi terhadap insektisida terutama terjadi karena adanya perubahan pada enzim metabolik serangga. Enzim metabolik yang sering berperan dalam kejadian resistensi antara lain adalah esterase dan monooksigenase. Metode: Uji kerentanan dan uji biokimia untuk mendeteksi resistensi terhadap malation dan cypermetrin dilakukan pada Aedes aegypti dari Wonosobo (daerah endemis baru infeksi Dengue di dataran tinggi). Uji coba yang dilakukan pada generasi F1 nyamuk Ae.aegypti yang tertangkap di lapangan bertujuan untuk mengetahui mekanisme resistensi berdasarkan aktivitas dua enzim detoksifikasi yaitu esterase dan monooksigenase. Wawancara dengan menggunakan kuesioner terstruktur dilakukan untuk mengetahui penggunaan insektisida oleh masyarakat dan pemerintah daerah. Hasil: Uji kerentanan menunjukkan mortalitas sebesar 23,4% setelah terpapar malathion 0,8% dan 46,7% setelah terpapar cypermethrin 0,05%. Hasil uji biokimia menunjukkan bahwa aktivitas esterase dan monooksigenase cenderung meningkat pada Ae.aegypti di Wonosobo. Wawancara dan kuesioner menyimpulkan bahwa cypermetrin adalah satu-satunya golongan insektisida yang digunakan dalam program pengendalian vektor oleh Dinas Kesehatan Wonosobo dan merupakan tipe insektisida yang paling sering digunakan di rumah tangga oleh masyarakat Wonosobo untuk mengendalikan populasi Ae.aegypti. Kesimpulan: Ditemukan nyamuk Ae. aegypti yang mengalami peningkatan aktivitas enzim esterase dan monooksigenase pada populasi Ae. aegypti di Kabupaten Wonosobo. Hal ini selaras dengan status resistensi populasi nyamuk tersebut yang resisten terhadap Malation dan Cypermetrin. Kata kunci: Ae.aegypti Wonosobo, biokimia, paparan insektisida, resistensi Abstract Background: Resistance to insecticides mainly occurs due to changes in insect metabolic enzyme. A metabolic enzyme which was often involved in insecticide resistance is esterase and monooxygenase. Methods: Susceptibility test and biochemical assay to detect malathion and cypermethrin resistance were conducted on Aedes aegypti from Wonosobo (new highland Dengue endemic area). The test was performed on F1 generation of Ae.aegypti field caught mosquitoes which aimed to determine the resistance mechanisms regarding two detoxifying enzymes i.e. esterase and monooxygenase. Interview using structured questionnaires was conducted to investigate the usage of insecticide by the society and local government. Results: Susceptibility test showed 23.4 and 46.7% mortalities after exposure to 0.8% malathion and 0.05% cypermethrin. The biochemical assay result suggested that esterase, and monooxygenase activity tend to increase in Ae.aegypti in Wonosobo. Interview and questionnaires conclude that synthetic pyrethroid was the only insecticide type used in vector control program by Wonosobo Health Office and was the most frequent insecticide type to be used in household by Wonosobo society to control Ae.aegypti population. Conclusion: Aedes aegypti with increased esterase
{"title":"Biochemical characterization of insecticide resistance and exposure in Aedes aegypti population from Wonosobo (a new highland Dengue endemic area), Central Java, Indonesia","authors":"Dyah Widiastuti, B. Ikawati, M. Martini, N. Wijayanti","doi":"10.22435/hsji.v8i2.6854.","DOIUrl":"https://doi.org/10.22435/hsji.v8i2.6854.","url":null,"abstract":"Latar Belakang: Resistensi terhadap insektisida terutama terjadi karena adanya perubahan pada enzim metabolik serangga. Enzim metabolik yang sering berperan dalam kejadian resistensi antara lain adalah esterase dan monooksigenase. Metode: Uji kerentanan dan uji biokimia untuk mendeteksi resistensi terhadap malation dan cypermetrin dilakukan pada Aedes aegypti dari Wonosobo (daerah endemis baru infeksi Dengue di dataran tinggi). Uji coba yang dilakukan pada generasi F1 nyamuk Ae.aegypti yang tertangkap di lapangan bertujuan untuk mengetahui mekanisme resistensi berdasarkan aktivitas dua enzim detoksifikasi yaitu esterase dan monooksigenase. Wawancara dengan menggunakan kuesioner terstruktur dilakukan untuk mengetahui penggunaan insektisida oleh masyarakat dan pemerintah daerah. Hasil: Uji kerentanan menunjukkan mortalitas sebesar 23,4% setelah terpapar malathion 0,8% dan 46,7% setelah terpapar cypermethrin 0,05%. Hasil uji biokimia menunjukkan bahwa aktivitas esterase dan monooksigenase cenderung meningkat pada Ae.aegypti di Wonosobo. Wawancara dan kuesioner menyimpulkan bahwa cypermetrin adalah satu-satunya golongan insektisida yang digunakan dalam program pengendalian vektor oleh Dinas Kesehatan Wonosobo dan merupakan tipe insektisida yang paling sering digunakan di rumah tangga oleh masyarakat Wonosobo untuk mengendalikan populasi Ae.aegypti. Kesimpulan: Ditemukan nyamuk Ae. aegypti yang mengalami peningkatan aktivitas enzim esterase dan monooksigenase pada populasi Ae. aegypti di Kabupaten Wonosobo. Hal ini selaras dengan status resistensi populasi nyamuk tersebut yang resisten terhadap Malation dan Cypermetrin. Kata kunci: Ae.aegypti Wonosobo, biokimia, paparan insektisida, resistensi Abstract Background: Resistance to insecticides mainly occurs due to changes in insect metabolic enzyme. A metabolic enzyme which was often involved in insecticide resistance is esterase and monooxygenase. Methods: Susceptibility test and biochemical assay to detect malathion and cypermethrin resistance were conducted on Aedes aegypti from Wonosobo (new highland Dengue endemic area). The test was performed on F1 generation of Ae.aegypti field caught mosquitoes which aimed to determine the resistance mechanisms regarding two detoxifying enzymes i.e. esterase and monooxygenase. Interview using structured questionnaires was conducted to investigate the usage of insecticide by the society and local government. Results: Susceptibility test showed 23.4 and 46.7% mortalities after exposure to 0.8% malathion and 0.05% cypermethrin. The biochemical assay result suggested that esterase, and monooxygenase activity tend to increase in Ae.aegypti in Wonosobo. Interview and questionnaires conclude that synthetic pyrethroid was the only insecticide type used in vector control program by Wonosobo Health Office and was the most frequent insecticide type to be used in household by Wonosobo society to control Ae.aegypti population. Conclusion: Aedes aegypti with increased esterase","PeriodicalId":30666,"journal":{"name":"Health Science Journal of Indonesia","volume":"8 1","pages":"74-80"},"PeriodicalIF":0.0,"publicationDate":"2017-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45768835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-07-27DOI: 10.22435/hsji.v8i1.5600.
N. Nurhayati, L. Widowati
Latar belakang : Penggunaan obat tradisional, pengobatan komplementer dan alternatif meningkat selama beberapa tahun terakhir. Alasan utama meningkatnya penggunaan obat tradisional adalah pasien mengambil pendekatan yang lebih proaktif untuk kesehatan mereka dan mencari berbagai bentuk perawatan. Penelitian ini bertujuan untuk mengidentifikasi faktor dominan yang berhubungan dengan pemanfaatan pelayanan kesehatan tradisional pada rumah tangga di Indonesia Metode : Penelitian ini menggunakan data rumah tangga dari data Riskesdas tahun 2013. Jumlah data yang dianalisis sebesar 294.959 subjek. Analisis data menggunakan analisis kompleks sampel dengan regresi logistik untuk memperoleh faktor dominan yang berhubungan dengan pemanfaatan pelayanan kesehatan tradisional pada rumah tangga di Indonesia Hasil : Sebanyak 294.959 subjek pada penelitian ini. Proporsi subjek yang memanfaatkan pelayanan kesehatan tradisional adalah 30,4% (89.752/294.959). Faktor risiko dominan yang berhubungan dengan pemanfaatan pelayanan kesehatan tradisional adalah wilayah tempat tinggal, tingkat pendidikan, status pekerjaan, status ekonomi, dan ketersediaan pelayanan kesehatan. Dibandingkan dengan rumah tangga yang tinggal di pedesaan, rumahtangga yang berada di perkotaan berpeluang 1,09 kali memanfaatkan pelayanan kesehatan tradsional [rasio odds suaian (ORa)= 1,09; 95% CI= 1,04 to 1,14]. Rumah tangga yang tingkat pendidikannya rendah berpeluang 1,10 kali memanfaatkan pelayanan kesehatan tradisional (ORa=1,10; 95% CI=1,03 to 1,18). Rumah tangga dengan pekerjaan swasta berpeluang 1,33 kali memanfaatkan pelayanan kesehatan tradsional (ORa=1,33; 95% CI=1,25 to 1,41). Rumah tangga yang memiliki tingkat ekonomi tinggi berpeluang 1,31 kali memanfaatkan pelayanan kesehatan tradisional (ORa=1,31; 95% CI=1,23 to 1,41). Rumah tangga yang mengetahui ketersediaan pelayanan kesehatan berpeluang 1,44 kali memanfaatkan pelayanan kesehatan tradisional (ORa=1,44; 95% CI=1,29 to 1,60). Kesimpulan : Rumah tangga yang tinggal di perkotaan, memiliki tingkat pendidikan rendah, pekerja swasta, memiliki status ekonomi yang tinggi, mengetahui ketersediaan pelayanan kesehatan, lebih berpeluang memanfaatkan pelayanan kesehatan tradisional Kata kunci : pelayanan kesehatan tradisional, rumah tangga, Indonesia Background: The use of traditional medicine and complementary and alternative medicine has increased significantly over the past few years. The main reasons for the increasing use of traditional medicine is a growing trend for patients to take a more proactive approach to their own health and to seek out different forms of self-care. This study aimed to investigate the dominant risk factors that related to use of traditional health care among Indonesian family Methods : The study used households data from 2013 National Health Survey Indonesia. Using logistic regression, we then could present dominant risk factors that related to use of traditional health care among Indonesian family Results : Total
{"title":"The use of traditional health care among Indonesian Family","authors":"N. Nurhayati, L. Widowati","doi":"10.22435/hsji.v8i1.5600.","DOIUrl":"https://doi.org/10.22435/hsji.v8i1.5600.","url":null,"abstract":"Latar belakang : Penggunaan obat tradisional, pengobatan komplementer dan alternatif meningkat selama beberapa tahun terakhir. Alasan utama meningkatnya penggunaan obat tradisional adalah pasien mengambil pendekatan yang lebih proaktif untuk kesehatan mereka dan mencari berbagai bentuk perawatan. Penelitian ini bertujuan untuk mengidentifikasi faktor dominan yang berhubungan dengan pemanfaatan pelayanan kesehatan tradisional pada rumah tangga di Indonesia Metode : Penelitian ini menggunakan data rumah tangga dari data Riskesdas tahun 2013. Jumlah data yang dianalisis sebesar 294.959 subjek. Analisis data menggunakan analisis kompleks sampel dengan regresi logistik untuk memperoleh faktor dominan yang berhubungan dengan pemanfaatan pelayanan kesehatan tradisional pada rumah tangga di Indonesia Hasil : Sebanyak 294.959 subjek pada penelitian ini. Proporsi subjek yang memanfaatkan pelayanan kesehatan tradisional adalah 30,4% (89.752/294.959). Faktor risiko dominan yang berhubungan dengan pemanfaatan pelayanan kesehatan tradisional adalah wilayah tempat tinggal, tingkat pendidikan, status pekerjaan, status ekonomi, dan ketersediaan pelayanan kesehatan. Dibandingkan dengan rumah tangga yang tinggal di pedesaan, rumahtangga yang berada di perkotaan berpeluang 1,09 kali memanfaatkan pelayanan kesehatan tradsional [rasio odds suaian (ORa)= 1,09; 95% CI= 1,04 to 1,14]. Rumah tangga yang tingkat pendidikannya rendah berpeluang 1,10 kali memanfaatkan pelayanan kesehatan tradisional (ORa=1,10; 95% CI=1,03 to 1,18). Rumah tangga dengan pekerjaan swasta berpeluang 1,33 kali memanfaatkan pelayanan kesehatan tradsional (ORa=1,33; 95% CI=1,25 to 1,41). Rumah tangga yang memiliki tingkat ekonomi tinggi berpeluang 1,31 kali memanfaatkan pelayanan kesehatan tradisional (ORa=1,31; 95% CI=1,23 to 1,41). Rumah tangga yang mengetahui ketersediaan pelayanan kesehatan berpeluang 1,44 kali memanfaatkan pelayanan kesehatan tradisional (ORa=1,44; 95% CI=1,29 to 1,60). Kesimpulan : Rumah tangga yang tinggal di perkotaan, memiliki tingkat pendidikan rendah, pekerja swasta, memiliki status ekonomi yang tinggi, mengetahui ketersediaan pelayanan kesehatan, lebih berpeluang memanfaatkan pelayanan kesehatan tradisional Kata kunci : pelayanan kesehatan tradisional, rumah tangga, Indonesia Background: The use of traditional medicine and complementary and alternative medicine has increased significantly over the past few years. The main reasons for the increasing use of traditional medicine is a growing trend for patients to take a more proactive approach to their own health and to seek out different forms of self-care. This study aimed to investigate the dominant risk factors that related to use of traditional health care among Indonesian family Methods : The study used households data from 2013 National Health Survey Indonesia. Using logistic regression, we then could present dominant risk factors that related to use of traditional health care among Indonesian family Results : Total","PeriodicalId":30666,"journal":{"name":"Health Science Journal of Indonesia","volume":"8 1","pages":"70300"},"PeriodicalIF":0.0,"publicationDate":"2017-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45326742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-07-26DOI: 10.22435/HSJI.V8I1.5341.36-42
C. Ariani
Abstrak Latar belakang : Kebiasaan melakukan latihan fisik antara lain berguna bagi kebugaran fisik serta kognitif, serta mencegah inkapasitasi akibat nyeri punggung bawah, kelelahan, dan penyakit kardiovaskular. Penelitian ini bertujuan untuk mengetahui gambaran kebiasaan latihan fisik pada pilot sipil di Indonesia serta faktor-faktor yang mempengaruhinya. Metode : Penelitian ini dilakukan dengan desain potong lintang menggunakan data sekunder dari Survey Terhadap Kebiasaan Hidup Sehat Pilot Sipil di Indonesia Tahun 2016. Pada penelitian ini, yang dianalisis adalah data pilot dengan lisensi CPL dan ATPL. Kebiasaan latihan fisik sesuai dengan rekomendasi latihan fisik American College of Sports Medicine (ACSM) . Data yang diambil meliputi data demografi, pekerjaan, kepuasan hidup, indeks massa tubuh, dan lingkar pinggang. Analisis regresi Cox digunakan untuk menganalisis faktor-faktor dominan yang mempengaruhi kebiasaan latihan fisik. Hasil : Di antara 644 data pilot, terdapat 332 data yang memenuhi kriteria. Proporsi pilot sipil yang memiliki kebiasaan latihan fisik yang sesuai rekomendasi adalah 44%. Faktor dominan yang mempengaruhi latihan fisik adalah usia. Pilot sipil berusia 50 sampai 65 tahun memiliki kebiasaan latihan fisik 40% lebih rendah dibandingkan dengan pilot sipil berusia 20 – 29 tahun (risiko relatif suaian (RRa) 0,60; Interval Kepercayaan (IK) 95% 0,38 – 0,94; P 0,026). Sedangkan jam terbang di luar jadwal dan indeks massa tubuh yang berlebih nampaknya berpengaruh terhadap kebiasaan latihan fisik (secara berurutan: RRa 0,58; IK95% 0,30 – 1,13; P 0,112 dan RRa 0,79; IK95% 0,62 – 1,02; P 0,072). Kesimpulan : Usia, jam terbang di luar jadwal, dan IMT berlebih nampaknya berpengaruh terhadap kebiasan latihan fisik pada pilot sipil di Indonesia. Kata kunci : latihan fisik, jam terbang, pilot sipil, Indonesia Abstract Background: Physical exercise habit has some benefits, among others, for physical fitness and cognitive function, as well as preventing incapacitation events caused by low back pain, fatigue, dan cardiovascular diseases. The aim of this study was to find out the description of physical exercise habit among commercial pilot in Indonesia and its affecting factors. Method: This study was conducted with cross sectional design using secondary data from Healthy Lifestyle Survey of Commercial Pilot in Indonesia Year 2016. In this study, data of pilots with CPL and ATPL was analysed. Physical exercise habit was defined appropriately with American College of Sports Medicine (ACSM) recommendation . Data taken were demographic data, job factors, satisfaction of life, body mass index, and waist circumference. Cox regression analysis was used to analyse dominant factors which affect physical exercise habit. Result s : Among 644 pilots’ data, there were 332 data met the criteria in this study. Proportion of commercial pilots who had appropriate exercise habit was 44%. Dominant factor which affecting exercise habit was age. Compared to
{"title":"Flight hours of unplanned flight and other risk factors affecting exercise habit among commercial pilots in Indonesia","authors":"C. Ariani","doi":"10.22435/HSJI.V8I1.5341.36-42","DOIUrl":"https://doi.org/10.22435/HSJI.V8I1.5341.36-42","url":null,"abstract":"Abstrak Latar belakang : Kebiasaan melakukan latihan fisik antara lain berguna bagi kebugaran fisik serta kognitif, serta mencegah inkapasitasi akibat nyeri punggung bawah, kelelahan, dan penyakit kardiovaskular. Penelitian ini bertujuan untuk mengetahui gambaran kebiasaan latihan fisik pada pilot sipil di Indonesia serta faktor-faktor yang mempengaruhinya. Metode : Penelitian ini dilakukan dengan desain potong lintang menggunakan data sekunder dari Survey Terhadap Kebiasaan Hidup Sehat Pilot Sipil di Indonesia Tahun 2016. Pada penelitian ini, yang dianalisis adalah data pilot dengan lisensi CPL dan ATPL. Kebiasaan latihan fisik sesuai dengan rekomendasi latihan fisik American College of Sports Medicine (ACSM) . Data yang diambil meliputi data demografi, pekerjaan, kepuasan hidup, indeks massa tubuh, dan lingkar pinggang. Analisis regresi Cox digunakan untuk menganalisis faktor-faktor dominan yang mempengaruhi kebiasaan latihan fisik. Hasil : Di antara 644 data pilot, terdapat 332 data yang memenuhi kriteria. Proporsi pilot sipil yang memiliki kebiasaan latihan fisik yang sesuai rekomendasi adalah 44%. Faktor dominan yang mempengaruhi latihan fisik adalah usia. Pilot sipil berusia 50 sampai 65 tahun memiliki kebiasaan latihan fisik 40% lebih rendah dibandingkan dengan pilot sipil berusia 20 – 29 tahun (risiko relatif suaian (RRa) 0,60; Interval Kepercayaan (IK) 95% 0,38 – 0,94; P 0,026). Sedangkan jam terbang di luar jadwal dan indeks massa tubuh yang berlebih nampaknya berpengaruh terhadap kebiasaan latihan fisik (secara berurutan: RRa 0,58; IK95% 0,30 – 1,13; P 0,112 dan RRa 0,79; IK95% 0,62 – 1,02; P 0,072). Kesimpulan : Usia, jam terbang di luar jadwal, dan IMT berlebih nampaknya berpengaruh terhadap kebiasan latihan fisik pada pilot sipil di Indonesia. Kata kunci : latihan fisik, jam terbang, pilot sipil, Indonesia Abstract Background: Physical exercise habit has some benefits, among others, for physical fitness and cognitive function, as well as preventing incapacitation events caused by low back pain, fatigue, dan cardiovascular diseases. The aim of this study was to find out the description of physical exercise habit among commercial pilot in Indonesia and its affecting factors. Method: This study was conducted with cross sectional design using secondary data from Healthy Lifestyle Survey of Commercial Pilot in Indonesia Year 2016. In this study, data of pilots with CPL and ATPL was analysed. Physical exercise habit was defined appropriately with American College of Sports Medicine (ACSM) recommendation . Data taken were demographic data, job factors, satisfaction of life, body mass index, and waist circumference. Cox regression analysis was used to analyse dominant factors which affect physical exercise habit. Result s : Among 644 pilots’ data, there were 332 data met the criteria in this study. Proportion of commercial pilots who had appropriate exercise habit was 44%. Dominant factor which affecting exercise habit was age. Compared to ","PeriodicalId":30666,"journal":{"name":"Health Science Journal of Indonesia","volume":"8 1","pages":"36-42"},"PeriodicalIF":0.0,"publicationDate":"2017-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42231065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstrak Latar belakang Tidak tersedianya waktu merupakan salah satu hambatan melakukan latihan fisik yang sering dilaporkan di negara berkembang. Berdasarkan Peraturan Keselamatan Penerbangan Sipil bagian 121, jam terbang maksimal pilot sipil komersial dalam 7 hari terakhir adalah 30 jam. Oleh karena itu perlu dilakukan penelitian mengenai hubungan jam terbang 7 hari terakhir terhadap kebiasaan latihan fisik pada pilot sipil di Indonesia. Metode Penelitian potong lintang terhadap 600 orang pilot sipil yang melakukan pengujian kesehatan personil penerbangan di Balai Kesehatan Penerbangan pada bulan April 2016 dan memenuhi kriteria inklusi/ekslusi. Data yang dikumpulkan yaitu karakteristik demografi, pekerjaan, kebiasaan olahraga, tinggi dan berat badan. Pengambilan data dilakukan dengan wawancara dan pemeriksaan fisik. Pilot dikategorikan memiliki kebiasaan latihan fisik sesuai rekomendasi ACSM apabila melakukan latihan fisik dengan intensitas sedang selama 150 menit per minggu atau latihan fisik dengan intensitas berat selama 75 menit per minggu. Hasil Jam terbang 7 hari terakhir merupakan faktor dominan terhadap kebiasaan latihan fisik. Jika dibandingkan dengan pilot dengan jam terbang 7 hari terakhir < 3,5 jam, maka pilot dengan jam terbang 7 hari terakhir 3,5-14 jam berisiko 24% lebih rendah memiliki kebiasaan latihan fisik sesuai [RRa= 0,76; p=0,032]. Simpulan Faktor risiko yang berpengaruh terhadap kebiasaan latihan fisik adalah jam terbang 7 hari terakhir. Kata kunci: Jam terbang 7 hari terakhir, kebiasaan latihan fisik, pilot sipil Indonesia Background The most frequently reported barrier of doing physical exercise in developed countries is lack of time. Based on the Civil Aviation Safety Regulation part 121, the maximum working hour for commercial pilot in 7 consecutive days is 30 hours. The study objective is to identify the relation between flight hours in 7 consecutive days and the physical exercise habit among the civil pilots in Indonesia. Method A cross sectional study towards 600 civil pilots, who did their medical examination at the Aviation Medical Center, Jakarta and met the inclusion/exclusion criteria. The collected data were demographic and job characteristics, physical exercise habits, body weight, heights. The data was obtained through interview and physical examinations. Pilot was categorized as having an appropriate physical exercise habit if he/she spent 150 minutes of moderate intensity exercise per week or 75 minutes of vigorous intensity exercise per week. Results Flight hour in 7 consecutive days was a dominant factor to the physical exercise habit. Compared to the pilot who had flight hours in 7 consecutive days less than 3.5 hours, pilot who had flight hours in 7 consecutive days 3.5-14 were having less 24% risk of appropriate physical exercise [RRa= 0,76; p=0,032]. Conclusion Risk factor to the physical exercise habit among the civil pilot is flight hour in 7 consecutive days. Key words: Flight hours in 7 consecu
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Yuli Arisanti Sitanggang, H. Hutapea, S. Suhardi, Y. Maladan, T. Wahyuni, Muhammad Fajri Rokmad
Latar belakang : Penyakit frambusia masih menjadi masalah yang belum terselesaikan di Kota Jayapura sebab terdapat daerah kantong frambusia di Kota Jayapura. Penelitian ini bertujuan untuk mendapatkan data status frambusia secara serologi dan faktor risikonya. Metode : Penelitian deskriptif yang dilakukan secara potong lintang. Pemeriksaan laboratorium standar yang digunakan berupa uji Treponema pallidum haemagglutination (TPHA) pada 322 kasus yang didiagnosis Yaws di Puskesmas Hamadi dari Januari – Oktober 2016. Hasil: Faktor risiko frambusia berupa riwayat pernah mengalami frambusia memiliki potensi dua kali lebih besar untuk mengalami frambusia kembali. Faktor risiko frambusia meningkat dua kali lebih besar pada kasus yang kurang berperilaku hidup bersih dan sehat yaitu: jarang mandi dan tanpa menggunakan sabun dan mempunyai kebiasaan jarang/tidak pakai sandal. Potensi risiko meningkat tiga kali pada kebiasaan pakai handuk bersama. Kesimpulan: Secara serologi dengan TPHA yang positif didapat pada 111 sampel dari kasus dan kontak. Periaku hidup yang kurang bersih dan sehat merupakan faktor risiko yang mempengaruhi kejadian frambusia. Kata kunci: TPHA, frambusia, faktor risiko, perilaku hidup bersih dan sehat Background: Yaws remained an unfinished health problem in Jayapura City, as there still yaws case found. This research aimed to obtain serological prevalence data and determine the risk factors for yaws transmission in Jayapura. Methods : These was descriptive and cross-sectional research, and TPHA was the standard laboratory test used during the research. Results : The risk factor of a frambusia in the history of having experienced frambusia has twice as much potential for experiencing frambusia again. The risk factor of frambusia has increased twice as much in less healthy behaviors: rarely bathing and without using soap and having a rare / non-slip habit. Potential risk increased three times in the habit of sharing a towel together. Conclusion : Serology with positive TPHA was obtained in 111 samples of cases and contacts. Not clean and healthy behavior is a risk factor that affects the incidence of frambusia. Keywords : TPHA, yaws, risk factor, personal hygiene
{"title":"Serologic observation and risk factor of yaws in Hamadi Public Health Center, Jayapura","authors":"Yuli Arisanti Sitanggang, H. Hutapea, S. Suhardi, Y. Maladan, T. Wahyuni, Muhammad Fajri Rokmad","doi":"10.22435/hsji.v8i1.6387","DOIUrl":"https://doi.org/10.22435/hsji.v8i1.6387","url":null,"abstract":"Latar belakang : Penyakit frambusia masih menjadi masalah yang belum terselesaikan di Kota Jayapura sebab terdapat daerah kantong frambusia di Kota Jayapura. Penelitian ini bertujuan untuk mendapatkan data status frambusia secara serologi dan faktor risikonya. Metode : Penelitian deskriptif yang dilakukan secara potong lintang. Pemeriksaan laboratorium standar yang digunakan berupa uji Treponema pallidum haemagglutination (TPHA) pada 322 kasus yang didiagnosis Yaws di Puskesmas Hamadi dari Januari – Oktober 2016. Hasil: Faktor risiko frambusia berupa riwayat pernah mengalami frambusia memiliki potensi dua kali lebih besar untuk mengalami frambusia kembali. Faktor risiko frambusia meningkat dua kali lebih besar pada kasus yang kurang berperilaku hidup bersih dan sehat yaitu: jarang mandi dan tanpa menggunakan sabun dan mempunyai kebiasaan jarang/tidak pakai sandal. Potensi risiko meningkat tiga kali pada kebiasaan pakai handuk bersama. Kesimpulan: Secara serologi dengan TPHA yang positif didapat pada 111 sampel dari kasus dan kontak. Periaku hidup yang kurang bersih dan sehat merupakan faktor risiko yang mempengaruhi kejadian frambusia. Kata kunci: TPHA, frambusia, faktor risiko, perilaku hidup bersih dan sehat Background: Yaws remained an unfinished health problem in Jayapura City, as there still yaws case found. This research aimed to obtain serological prevalence data and determine the risk factors for yaws transmission in Jayapura. Methods : These was descriptive and cross-sectional research, and TPHA was the standard laboratory test used during the research. Results : The risk factor of a frambusia in the history of having experienced frambusia has twice as much potential for experiencing frambusia again. The risk factor of frambusia has increased twice as much in less healthy behaviors: rarely bathing and without using soap and having a rare / non-slip habit. Potential risk increased three times in the habit of sharing a towel together. Conclusion : Serology with positive TPHA was obtained in 111 samples of cases and contacts. Not clean and healthy behavior is a risk factor that affects the incidence of frambusia. Keywords : TPHA, yaws, risk factor, personal hygiene","PeriodicalId":30666,"journal":{"name":"Health Science Journal of Indonesia","volume":"8 1","pages":"25-29"},"PeriodicalIF":0.0,"publicationDate":"2017-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48440623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Anggraini, E. Koendhori, H. Pramono, Daniel Djoko Wahyono
Latar belakang: Analisis polimorfisme suatu gen penting dilakukan untuk memperoleh informasi lebih awal dalam identifikasi penanda genetik yang berhubungan dengan sifat yang ingin dilihat. Metode RFLP menjadi salah satu metode yang dipilih karena dapat melihat polimorfisme urutan DNA yang dapat dideteksi melalui adanya perbedaan fragmen DNA setelah dipotong dengan menggunakan enzim endonuclease tertentu sehingga mampu menggambarkan polimorfisme dari suatu gen. Tujuan penelitian adalah untuk mengetahui adanya polimorfisme gen coagulase S. aureus resisten methicillin. Metode: Penelitian yang dilakukan secara deskriptif pada 25 sampel. Isolatbakteri MRSA diidentifikasi menggunakan pemeriksaan bakteriologis dan PCR gen mecA dan coagulase dengan menggunakan primer spesifik. Analisis polimorfisme gen coagulase dengan situs restriksi AluI isolat S. aureus resisten methicillin dilakukan dengan menggunakan metoda PCR-RFLP. Hasil: Amplifikasi menunjukkan produk PCR (amplicon) gen mecA dan coagulase dengan primer spesifik ke 25 isolate bakteri MRSA mempunyai positivitas sebesar 100% . Hasil dari PCR-RFLP menunjukkan empat pola RFLP dengan situs restriksi AluI pada 25 isolat. Proposi terbesar (64%) pada pola RFLP I (pola yang tidak terdigesti enzim restriksi AluI). Kesimpulan: Terdapat polimorfisme isolate S. aureus resisten methicillin berdasarkan analisis gen coagulase . Kata kunci: Staphylococcus aureus,gen coagulase, PCR-RFLP, AluI Background: Analysis of the polymorphism of a gene is important to obtain early information in identifying genetic markers related to the characteristics to be seen.The RFLP method becomes one of the chosen methods because it can see polymorphism that can be detected by using the different fragments of DNA that have been cut by using certain endonuclease enzyme so that it is possible to describe the polymorphism of a gene.The aim of the study is to discover the gene polymorphism of methicillin-resistant Staphylococcus aureus. Methods: This was a descriptive study using 25 isolates. Isolates of MRSA tested bybacteriological examination and PCR of mecA and coagulase gene using specific primers.Polymorphism analysis of the coagulase gene in isolates of methicillin-resistant Staphylococcusaureus with AluI Restriction Sites tested by PCR-RFLP. Results: The Amplification showed that PCR product (amplicon) of mecA and coagulase gene from specific primers of all 25 isolate samples, had a positivity of 100%.The PCR-RFLP of coagulase gene showed that all 25 samples underwent polymorphism into four RFLP patterns with AluI restriction sites. The largest proportion (64%) was found polymorphism in clinical samples MRSA with RFLP I pattern (un-digested pattern of AluI restriction enzyme). Conclusion: There is polymorphism in the samples MRSA from the analysis of the coagulase gene. Keywords: Staphylococcus aureus, coagulase gene, PCR-RFLP, AluI
{"title":"Polymorphism Analysis of the Coagulase Gene in Isolates of Methicillin-Resistant Staphylococcus aureus with AluI Restriction Sites","authors":"A. Anggraini, E. Koendhori, H. Pramono, Daniel Djoko Wahyono","doi":"10.22435/HSJI.V8I1.6865","DOIUrl":"https://doi.org/10.22435/HSJI.V8I1.6865","url":null,"abstract":"Latar belakang: Analisis polimorfisme suatu gen penting dilakukan untuk memperoleh informasi lebih awal dalam identifikasi penanda genetik yang berhubungan dengan sifat yang ingin dilihat. Metode RFLP menjadi salah satu metode yang dipilih karena dapat melihat polimorfisme urutan DNA yang dapat dideteksi melalui adanya perbedaan fragmen DNA setelah dipotong dengan menggunakan enzim endonuclease tertentu sehingga mampu menggambarkan polimorfisme dari suatu gen. Tujuan penelitian adalah untuk mengetahui adanya polimorfisme gen coagulase S. aureus resisten methicillin. Metode: Penelitian yang dilakukan secara deskriptif pada 25 sampel. Isolatbakteri MRSA diidentifikasi menggunakan pemeriksaan bakteriologis dan PCR gen mecA dan coagulase dengan menggunakan primer spesifik. Analisis polimorfisme gen coagulase dengan situs restriksi AluI isolat S. aureus resisten methicillin dilakukan dengan menggunakan metoda PCR-RFLP. Hasil: Amplifikasi menunjukkan produk PCR (amplicon) gen mecA dan coagulase dengan primer spesifik ke 25 isolate bakteri MRSA mempunyai positivitas sebesar 100% . Hasil dari PCR-RFLP menunjukkan empat pola RFLP dengan situs restriksi AluI pada 25 isolat. Proposi terbesar (64%) pada pola RFLP I (pola yang tidak terdigesti enzim restriksi AluI). Kesimpulan: Terdapat polimorfisme isolate S. aureus resisten methicillin berdasarkan analisis gen coagulase . Kata kunci: Staphylococcus aureus,gen coagulase, PCR-RFLP, AluI Background: Analysis of the polymorphism of a gene is important to obtain early information in identifying genetic markers related to the characteristics to be seen.The RFLP method becomes one of the chosen methods because it can see polymorphism that can be detected by using the different fragments of DNA that have been cut by using certain endonuclease enzyme so that it is possible to describe the polymorphism of a gene.The aim of the study is to discover the gene polymorphism of methicillin-resistant Staphylococcus aureus. Methods: This was a descriptive study using 25 isolates. Isolates of MRSA tested bybacteriological examination and PCR of mecA and coagulase gene using specific primers.Polymorphism analysis of the coagulase gene in isolates of methicillin-resistant Staphylococcusaureus with AluI Restriction Sites tested by PCR-RFLP. Results: The Amplification showed that PCR product (amplicon) of mecA and coagulase gene from specific primers of all 25 isolate samples, had a positivity of 100%.The PCR-RFLP of coagulase gene showed that all 25 samples underwent polymorphism into four RFLP patterns with AluI restriction sites. The largest proportion (64%) was found polymorphism in clinical samples MRSA with RFLP I pattern (un-digested pattern of AluI restriction enzyme). Conclusion: There is polymorphism in the samples MRSA from the analysis of the coagulase gene. Keywords: Staphylococcus aureus, coagulase gene, PCR-RFLP, AluI","PeriodicalId":30666,"journal":{"name":"Health Science Journal of Indonesia","volume":"8 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2017-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46777401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Subangkit Subangkit, Mursinah Mursinah, Rudi Putranto, V. Setiawaty
Latar Belakang: Campak adalah salah satu penyakit menular dan dapat menyebabkan penyakit serius sampai kematian. Campak masih merupakan masalah kesehatan masyarakat karena wabah campak masih terjadi di seluruh Indonesia. Surveilans berbasis laboratorium berikut penyelidikan epidemiologi molekuler memiliki kontribusi besar untuk mencegah wabah campak. Studi sebelumnya telah mendokumentasikan kehadiran genotipe virus campak G2, G3 dan D9 di Indonesia, dan genotipe lainnya seperti B3, D4, D5, D8 dan H1 telah terdeteksi di negara-negara tetangga. Penelitian ini bertujuan untuk mengkarakterisasi virus campak yang menyebabkan wabah di Indonesia pada tahun 2014. Metode: Tujuh puluh empat spesimen urin yang dikumpulkan dari delapan provinsi dan diperiksa oleh satu langkah RT-PCR dan metode sequencing Sanger. A nalisis sekuensing dilakukan menggunakan Bioedit 7.1; DNAstar 7.0 dan software MEGA5.0. Hasil: Hasil PCR menunjukkan 34 dari 74 spesimen klinis positif dari virus campak. Kami menemukan genotipe dari 34 virus campak milik genotipe D8, D9 dan G3. Kesimpulan: Campak pertama genotipe D8 telah terdeteksi dari Indonesia pada tahun 2014 meskipun campak lainnya genotipe masih dapat ditemukan di Indonesia. Kata kunci: virus campak, D8 genotipe, wabah, Indonesia Background : Measles is a highly contagious viral disease. It remains an important cause of death among young children globally, despite the availability of a safe and effective vaccine. Measles is still a public health problem in Indonesia and measles outbreak still reported from many areas throughout Indonesia.. Molecular epidemiology of measles viruses is an important component in outbreak investigations to to monitor the presence of circulating wild–type measles strains.Previous studies in Indonesia have documented the presence of measles virus genotypes G2, G3 and D9 in Indonesia, and the other genotypes such as B3, D4, D5, D8 and H1 have been detected in neighboring countries. This study aims to characterize the measles virus that causing outbreak in Indonesia in 2014. Methods : Seventy four urine specimens were collected from eight provinces and examined by one step RT-PCR and Sanger sequencing method. Sequencing analysis were conducted using Bioedit 7.1; DNA Star 7.0 and MEGA 5.0 software. Results : The PCR results showed 34 out of 74 clinical specimens positive of measles virus. We found the genotype of 34 measles viruses belongs to genotype D8, D9 and G3. Conclusion : The first measles genotype D8 has been detected from Indonesia in 2014 although other measles genotype still can be found in Indonesia. Keywords: Measles virus, D8 genotype, outbreak, Indonesia
背景：咽喉炎是一种可导致严重疾病致死的传染病。坎帕克仍然是一个公共卫生问题，因为它仍然发生在整个印度尼西亚。分子流行病学研究之后的实验室监测对预防疫情有重大贡献。先前的研究已经记录了G2、G3和D9在印度尼西亚的爆发，在邻国也检测到了B3、D4、D5、D8和H1等其他基因型。这项研究旨在装饰2014年在印度尼西亚爆发的疫情病毒。方法：从8个省抽取74份尿液样本，采用RT-PCR和Sanger测序方法进行检测。使用Bioedit 7.1进行的序列分析；DNAstar 7.0和MEGA5.0软件。结果：PCR结果显示，74份临床标本中有34份呈阳性。我们发现了34种废弃病毒的基因型，属于基因型D8、D9和G3。结论：2014年在印度尼西亚首次检测到D8基因型的释放，尽管在印度尼西亚仍能检测到其他基因型。关键词：坎帕克病毒，D8基因型，爆发，印度尼西亚背景：麻疹是一种高度传染性的病毒性疾病。尽管有安全有效的疫苗，但它仍然是全球幼儿死亡的重要原因。麻疹仍然是印度尼西亚的一个公共卫生问题，在整个印度尼西亚的许多地区仍有麻疹爆发的报告。。麻疹病毒的分子流行病学是监测流行的野生型麻疹毒株的爆发调查的重要组成部分。先前在印度尼西亚进行的研究表明，在印度尼西亚存在麻疹病毒基因型G2、G3和D9，在邻国也检测到其他基因型，如B3、D4、D5、D8和H1。本研究旨在描述2014年在印度尼西亚爆发的麻疹病毒。方法：采用一步RT-PCR和Sanger测序方法对8个省市的74份尿液标本进行检测。使用Bioedit 7.1进行测序分析；DNA Star 7.0和MEGA 5.0软件。结果：74例临床标本中，34例麻疹病毒阳性。我们发现34种麻疹病毒的基因型分别属于D8、D9和G3基因型。结论：2014年在印度尼西亚发现了第一个麻疹基因型D8，但在印度尼西亚仍能发现其他麻疹基因型。关键词：麻疹病毒，D8基因型，爆发，印度尼西亚
{"title":"Detection of Genotype D8 Measles Virus in Indonesia in 2014","authors":"Subangkit Subangkit, Mursinah Mursinah, Rudi Putranto, V. Setiawaty","doi":"10.22435/hsji.v8i1.6445","DOIUrl":"https://doi.org/10.22435/hsji.v8i1.6445","url":null,"abstract":"Latar Belakang: Campak adalah salah satu penyakit menular dan dapat menyebabkan penyakit serius sampai kematian. Campak masih merupakan masalah kesehatan masyarakat karena wabah campak masih terjadi di seluruh Indonesia. Surveilans berbasis laboratorium berikut penyelidikan epidemiologi molekuler memiliki kontribusi besar untuk mencegah wabah campak. Studi sebelumnya telah mendokumentasikan kehadiran genotipe virus campak G2, G3 dan D9 di Indonesia, dan genotipe lainnya seperti B3, D4, D5, D8 dan H1 telah terdeteksi di negara-negara tetangga. Penelitian ini bertujuan untuk mengkarakterisasi virus campak yang menyebabkan wabah di Indonesia pada tahun 2014. Metode: Tujuh puluh empat spesimen urin yang dikumpulkan dari delapan provinsi dan diperiksa oleh satu langkah RT-PCR dan metode sequencing Sanger. A nalisis sekuensing dilakukan menggunakan Bioedit 7.1; DNAstar 7.0 dan software MEGA5.0. Hasil: Hasil PCR menunjukkan 34 dari 74 spesimen klinis positif dari virus campak. Kami menemukan genotipe dari 34 virus campak milik genotipe D8, D9 dan G3. Kesimpulan: Campak pertama genotipe D8 telah terdeteksi dari Indonesia pada tahun 2014 meskipun campak lainnya genotipe masih dapat ditemukan di Indonesia. Kata kunci: virus campak, D8 genotipe, wabah, Indonesia Background : Measles is a highly contagious viral disease. It remains an important cause of death among young children globally, despite the availability of a safe and effective vaccine. Measles is still a public health problem in Indonesia and measles outbreak still reported from many areas throughout Indonesia.. Molecular epidemiology of measles viruses is an important component in outbreak investigations to to monitor the presence of circulating wild–type measles strains.Previous studies in Indonesia have documented the presence of measles virus genotypes G2, G3 and D9 in Indonesia, and the other genotypes such as B3, D4, D5, D8 and H1 have been detected in neighboring countries. This study aims to characterize the measles virus that causing outbreak in Indonesia in 2014. Methods : Seventy four urine specimens were collected from eight provinces and examined by one step RT-PCR and Sanger sequencing method. Sequencing analysis were conducted using Bioedit 7.1; DNA Star 7.0 and MEGA 5.0 software. Results : The PCR results showed 34 out of 74 clinical specimens positive of measles virus. We found the genotype of 34 measles viruses belongs to genotype D8, D9 and G3. Conclusion : The first measles genotype D8 has been detected from Indonesia in 2014 although other measles genotype still can be found in Indonesia. Keywords: Measles virus, D8 genotype, outbreak, Indonesia","PeriodicalId":30666,"journal":{"name":"Health Science Journal of Indonesia","volume":"8 1","pages":"7-11"},"PeriodicalIF":0.0,"publicationDate":"2017-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45502917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L. Saptawati, R. Febrinasari, R. D. Yudhani, Hudi Yono, A. Faza, Sarah Luthfiani, Hutami Sri Ummiyati, T. M. Sudiro, B. Dewi
Background: Dengue hemorrhagic fever (DHF) caused by a dengue viruses is still a major problem in tropical countries, including Indonesia. World Health Organization data showed that over 40% of world population are at risk of DHF.1In 2014 there were 71.668 of DHF cases in 34 provinces with 641 death.2 In Central Java in 2013, the incidence rate and fatality rate of DHF was 45.52 in 100.000 populations and 1.21% respectively.3 Until nowadays, there is no vaccine or effective therapy is available as yet.4 Thus research on discovering specific antiviral against dengue is needed. Indonesia is rich in indigenous herbal plants, which may has potential antiviral activity, such as Psidium guajava (Jambu biji), Euphorbia hirta (Patikn kerbau), Piper bettle L (Sirih), Carica papaya (Pepaya), Curcuma longa L(Kunyit/turmeric), Phyllanthus niruri L (meniran), Andrographis paniculata (Sambiloto), Cymbopogon citrates (Serai). Previous studies show that these plants have antiviral and antibacterial properties.5However, there is only limited study of these plants against dengue virus . Objective: This study aimed to know whether these plants have potential activity against dengue virus in vitro. Method: Leave extracts of eight indigenous herbal plants as mention before were originated from Solo, Central Java, the crude extracts were tested in vitro against dengue virus serotype 2 (DENV-2) strain NGC using Huh7it-1 cell line. Those crude extracts were screened for antiviral activity using doses of 20mg/ml. Candidates that showed inhibition activity were further tested in various doses to determine IC50 and CC50. Result: From eight leave extracts tested, one of them i.e Carica papaya (pepaya) inhibited virus replication up to 89,5%. Dose dependent assay with C.papaya resulted in IC50, CC50 and selectivity index 6,57 μg/mL, 244,76 μg/mL and 37, 25 μg/mL respectively. Conclusion: C.papaya has potential antiviral activity against dengue virus in vitro. Further study is needed to confirm antiviral activity in vivo.
{"title":"In Vitro Study of Eight Indonesian Natural Extracts as Antiviral Against Dengue Virus","authors":"L. Saptawati, R. Febrinasari, R. D. Yudhani, Hudi Yono, A. Faza, Sarah Luthfiani, Hutami Sri Ummiyati, T. M. Sudiro, B. Dewi","doi":"10.22435/hsji.v8i1.6601","DOIUrl":"https://doi.org/10.22435/hsji.v8i1.6601","url":null,"abstract":"Background: Dengue hemorrhagic fever (DHF) caused by a dengue viruses is still a major problem in tropical countries, including Indonesia. World Health Organization data showed that over 40% of world population are at risk of DHF.1In 2014 there were 71.668 of DHF cases in 34 provinces with 641 death.2 In Central Java in 2013, the incidence rate and fatality rate of DHF was 45.52 in 100.000 populations and 1.21% respectively.3 Until nowadays, there is no vaccine or effective therapy is available as yet.4 Thus research on discovering specific antiviral against dengue is needed. Indonesia is rich in indigenous herbal plants, which may has potential antiviral activity, such as Psidium guajava (Jambu biji), Euphorbia hirta (Patikn kerbau), Piper bettle L (Sirih), Carica papaya (Pepaya), Curcuma longa L(Kunyit/turmeric), Phyllanthus niruri L (meniran), Andrographis paniculata (Sambiloto), Cymbopogon citrates (Serai). Previous studies show that these plants have antiviral and antibacterial properties.5However, there is only limited study of these plants against dengue virus . Objective: This study aimed to know whether these plants have potential activity against dengue virus in vitro. Method: Leave extracts of eight indigenous herbal plants as mention before were originated from Solo, Central Java, the crude extracts were tested in vitro against dengue virus serotype 2 (DENV-2) strain NGC using Huh7it-1 cell line. Those crude extracts were screened for antiviral activity using doses of 20mg/ml. Candidates that showed inhibition activity were further tested in various doses to determine IC50 and CC50. Result: From eight leave extracts tested, one of them i.e Carica papaya (pepaya) inhibited virus replication up to 89,5%. Dose dependent assay with C.papaya resulted in IC50, CC50 and selectivity index 6,57 μg/mL, 244,76 μg/mL and 37, 25 μg/mL respectively. Conclusion: C.papaya has potential antiviral activity against dengue virus in vitro. Further study is needed to confirm antiviral activity in vivo.","PeriodicalId":30666,"journal":{"name":"Health Science Journal of Indonesia","volume":"8 1","pages":"63814"},"PeriodicalIF":0.0,"publicationDate":"2017-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48516177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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